Muhammad Imran1*, Muhammad Mobashar2, Muhammad Irfan3, Shazia Hanif4, Sumaira Hanif5 and Muhammad Abubakar6
...) in lactating Nili-Ravi buffaloes. On the basis of nearly similar lactation stage and body weight, sixteen individually confined buffaloes were randomly blocked and allotted to four different dietary treatments. MOLM in proportion to CSC was supplemented along with maize silage as basal diet. Four dietary treatments having MOLM were 0.0, 0.71, 1.42 and 2.16 Kg DM/day used as replacement for CSC containing 2.16, 1.42, 0.71 a...
Arif Khan, Abdur Rahman, Shoail Akhtar, Siraj Uddin, Zahid Ullah and Nazir Ahmad
...ustify;"> Pakistani buffaloes beef were analyzed for various quality characteristics in relation to breed and age. A total of one hundred and eight beef samples from Longissimus dorsi muscle were collected from three age groups (i.e. group 1= 12-18 monts, 2 =18.1-36.0 months and 3= > 36 months) of Kundi and Nili Ravi breeds of buffalo. Samples were obtained from freshly preselected slaughtered <...
Mian Shamas Murtaza1, Aysha Sameen1, Nuzhat Huma1 and Fatma Hussain2
Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
... on post-thaw quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4&d...
KM Fakhrul-Islam, Mohammad Shah Jalal, Sonnet Poddr, Md Nurul Quader, Md Sahidur-Rahman, Avijit Dutta and Shuvo Mazumder
...isease affecting cattle, buffalo, sheep, goats, and wild animals around the globe. This highly contagious disease causes severe economic loss due to reduced productivity of the affected animals as well as increased mortality in calves and kids. In this study proportionate prevalence of FMD in cattle was estimated and the distribution of FMD according to different factors was evaluated. Both retrospective and prospective FMD clinical cases were included conside...
Neelum Andaleeb and Munir Khan
...Average milk produced by buffalo and cow was 180 and 135 liters per month, respectively. Average total costs of milk production of buffalo and cow were Rs.12835 and Rs.10070 per month respectively whereas the average net income per liter from the buffalo and cow milk was Rs. 29 and Rs. 5 thus benefit-cost ratios for buffalo and cow is1:1.43 and 1:1.07, r...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
...Three cases, including a buffalo and two cows were presented with complaint of persistent unilateral hind limb extension. The animals were unable to flex the limb during walk. Diagnosis was based on case history, clinical sign and clinical examination. Upward fixation of patella was diagnosed in all three animals. After careful palpation of the ligament, medial patellar desmotomy was performed in lateral recumbence, under local anesthesia achieved by 2% lignoc...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
... biochemical response in buffaloes to Gigantocotyle explanatum infection. Of the 558 slaughtered buffaloes from central Punjab, Pakistan, 18.99% were infected, and morphometric analyses were made of 115 mature amphistomes and gravid eggs with traditional microscopic methods on the basis of standardized measurements. Amphistomes were flattened and stained in Borax carmine, some were sectioned in the median sagittal pla...
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...bortus in cattle and buffaloes in the districts of Islamabad and Rawalpindi, Pakistan. A total of 120 serum samples were randomly collected from buffaloes and cattle (60 per species) at Civil Veterinary Hospitals, animal markets and peri-urban livestock holdings in Rawalpindi and Islamabad. Serum samples were initially screened by the Rose Bengal Plate Test (RBPT). RBPT positive samples were subjected to a B. abortus<...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...gestibility in Nili Ravi buffaloes. Four lactating Nili-Ravi buffaloes of 3rd and 4th parity and milk production of 8±2.3 kg/day were selected and offered a basal diet based on sorghum forage and concentrate ration supplemented with fibrolytic enzyme (Fibrozyme™, Alltech Inc. Company, USA) at four levels (T1 = 0, T2 = 10, T3 = 15 and T4 = 20 g/animal/day) in a 4 × 4 Latin Square chang...
Amir Ismail1, Muhammad Riaz1,2*, Saeed Akhtar1, Amjad Farooq3, Muhammad Arif Shahzad1 and Ahmad Mujtaba4
...the milk samples of cow, buffalo and goat. The level of Pb was found to exceed the maximum permissible limit (0.02 µg/g) set by Codex Alimentarius Commission in 53% milk samples. In 44% milk samples the level of Cd was found to exceed the permissible limit (0.0026 µg/g) set by International Dairy Federation (1979). The mean levels of Ni, Cu and Co were found in the normal ranges. The data for estimated daily intake of heavy metals through milk show...
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...AO staining protocol for buffalo sperm which has not been explored yet. Semen was collected from two mature buffalo bulls, diluted in tris citrate egg yolk based extender and cryopreserved using standard protocol. The semen straws were thawed at 37oC and stained using different protocols as followed: Protocol 1 (washing effect); washing either with normal saline (NS) or phosphate buffered saline (PBS), Protocol II...
Shahid Hussain Farooqi1,*, Muhammad Ijaz1, Muhammad Hassan Saleem1, Muhammad Imran Rashid2, Muhammad Oneeb2, Amjad Khan3, Amjad Islam Aqib1 and Shakeel Mahmood4
...nd one hundred and fifty buffaloes. Descriptive statistics with Pearson’s Chi-square test and regression model were applied to analyze the data. The results of study found Rhepicephalus the most prevalent genus followed by Heamaphysalis, Hyalomma, Dermacenter and Amblyomma with the prevalence of 78.50%, 10.33%, 10.08%, 0.67% and 0.42%, respectively. On species basis, R. (Boophilus) annulatus, R. (Boophilus) microplus, <...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...mic losses in cattle and buffalo. So rapid and accurate diagnosis of FMD is uttermost need of this time. Several lab.techniques are developed for rapid detection of FMD but these are expensive and time consuming. Elisa, cell culture, Reverse Transcriptase (RT-PCR) and real time PCR need specific high quality equipments in lab. Whereas Lamp PCR is simple and accurate test for the rapid diagnosis of FMD. This study has been done initially with FMD known serotype...
Kashif Prince1,*, M. Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Jawaria Ali Khan1, Nisar Ahmad3,Rais Ahmed2, Aamerzish Mushtaq4, Sajid Umar4 and Yung-Fu Chang5
...sk factors in cattle and buffaloes of district Kasur, Punjab. Selenium status was evaluated by Atomic Absorptions Spectrophotometery (AAS) with respect to sex of animals, geographical area, age of animals, herd size, stage of animals, production level and concentrate feeding. Selenium deficiency was also evaluated as a risk factor of mastitis and repeat breeding. About 48.43% cattle and 72.39% buffaloes were found positive i...
Abdul Hassan*, Muhammad Ishaq*, Nisar Ali Shah* and Arshad Farooq*
MILK PRODUCTION POTENTIAL IN KHYBER PAKHTUNKHWA
...ith 1579 lit of milk per buffalo per year, 663 lit of milk per cow per year, 95 lit milk per goat per year and 64 lit of milk per sheep per year. The data in comparison to the National and World average, is far below from probable production levels. Except in buffaloes and goats where the farm level production of milk is higher than the World and sheep where the farm level production of milk of Khyber Pakhtunkhwa is higher t...
Arshed Bashir*, Fatima Ahmad**, Irfan Mehmood*, Muhammad Qasim*, Mazher Abbas* and Sonila Hassan*
ECONOMICS OF RED MEAT PRODUCTION IN PUNJAB
... farms were dominated by buffaloes. The mean age, sale price and live weight of sold buffaloes was 2.8 years, Rs. 64963.8 and 157 kg, respectively whereas for cows the mean age was 3 years with average sale price of Rs. 65722.1 per cow and live weight of 210 kg. The cost of production of meat animals (buffaloes and cows) was higher in commercial farms (Rs. 67820.2 and Rs. 62773.5, respecti...
Khalid Mahmood Aujla* and Abid Hussain*
ECONOMICS OF MILK PRODUCTION OF MAJOR DAIRY BUFFALO BREEDS BY AGRO-ECOLOGICAL ZONES IN PAKISTAN
...eceived from major dairy buffalo breeds (Nili-Ravi and Kundhi) in various agro-ecological zones of Pakistan. For this purpose, 219 buffalo farmers were randomly selected from mixed and rice-wheat cropping zones of Punjab and Sindh provinces, mixed cropping zone of Khyber Pakhtunkhwa (KPK) province, coastal zone of Sindh and mountainousAJK. Of these, 155 and 64 were Nili-Ravi and Kundhi buffalo...
Ghazala Nawaz*, Muhammad Nawaz Malik**, Muhammad Hassan
Mushtaq***, Fraz Munir Ahmad****, Ali Abdullah Shah*****, Farooq
Iqbal******, Shinawar Waseem Ali*******, Zahida Fatima******** and
Amjad Khan*********
SURVEILLANCE OF BRUCELLOSIS IN LIVESTOCK IN RURAL COMMUNITIES OF PUNJAB, PAKISTAN
...ence per 1000 animals in buffaloes was (7.86%), significantly (P<0.01) higher than small ruminants (5.84%) and cattle (2.05%). Being a subtropical region, geographically significant (P<0.01) variation was observed in burden and pattern of brucellosis i.e., the highest prevalence was in Central (14.81%) followed by South (0.5%) and northern (0.4%) Punjab. Considering the high mean prevalence and its zoonotic importance it can be devastating if not control...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
...y weight etc. in cattle, buffalo, goat and sheep worldwide. LEP gene polymorphism and associations have not been extensively reported in Lohi sheep of Pakistan, so this study aimed to find any variations in gene sequence and possible association with growth rate to explore meat potential of this breed. A total of 18 Lohi animals were selected from the flock. After the DNA extraction from blood samples, a 1486 bp fragment of exon three was amplified and sequenc...
Muhammad Umair Sattar1, Aysha Sameen1,*, Nuzhat Huma1 and Muhammad Shahid2
Abid Hussain1,*, Mansur-ud-Din Ahmad2, Muhammad Hussan Mushtaq2, Mamoona Chaudhry2, Muhammad Sarwar Khan2, Michael Reichel3, Tanveer Hussain4, Amjad Khan2, MuhammadNisar2 and Imtiaz Ahmad Khan5
...inical mastitis in dairy buffaloes. The present study was conducted in two districts Lahore and Bhimber Azad Kashmir Pakistan. Total sample size for this survey was consisted of 1,036 lactating buffaloes. The screening of lactating buffaloes was performed with the California Mastitis Test. The overall prevalence of mastitis was recorded at 49%. Among mastitis cases the prevalence of clinic...
Abdul Majid Ujjal2, Sharmin Akter1* and Suman Paul1
...e hospital prevalence of buffalo diseases and to determine the temporal distribution of important infectious diseases of buffalo like as Foot and Mouth Disease (FMD) and Haemorrhagic Septicaemia (HS) at Upazila veterinary hospitals of Jaintapur, Gowainghat and Kanaighat upazilas of Sylhet district. From October, 2015 to September, 2017 a total of 1057 cases of buffalo diseases were recorde...
Gulsanga1, Zahin Anjum1, Sahib Alam2* and Farhat Shehzad1
...d P) contents of cow and buffalo muscle meat obtained from University Campus, Peshawar. The data showed that Cu, Zn, Pb and Ni concentrations in cow meat ranged from 7.27 to 14.81, 64.00 to 112.12, 3.30 to 6.25 and 0.73 to 2.53 mg/kg whereas the concentrations of these heavy metals in buffalo meat varied from 3.15 to 7.71, 72.88 to 88.47, 1.41 to 13.28 and 0.38 to 1.93 mg/kg, respectively. The average Na, K, Ca, Mg and P con...
Hasina Baloch1, Rahmatullah Rind2,*, Muhammad Rafique Rind3, Viram Kumar4, Nazia Baloch5 and Rajesh K. Oad6
... subclinical mastitis in buffaloes. Significantly higher prevalence (49. 07%) of clinical mastitis was observed in buffaloes with average age of 6 to 9 years whereas those of > 9 years of age showed higher (33.33%) but non-significant prevalence of subclinical mastitis. However, significant impact of the number of parity on the prevalence of clinical mastitis was observed in animals. Higher prevalence (48.20%) o...
Selçuk Kaplan
...tions in Anatolian water buffaloes. Sequence analysis were revealed seven polymorphic site (G1072A, T1081C, T1131G, T1143C, T1145G, T1151G and C1221T) and one monomorphic site (C1071T) in Anatolian water buffaloes. To the best of my knowledge, this is the first study that fully describes seven polymorphic sites of bubaline leptin gene in Anatolian water buffaloes.
...
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...le (Bos indicus), buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Capra hircus). Selected marker V158M is present in exon-8 of COMT gene. Each specie was subjected to blood sampling (n=20 each). DNA was extracted, purified, quantified and amplified through PCR by using specific primer set. Then Sanger’s method of DNA sequencing was used. Multiple sequence alignment demonstrated that sheep and goat were having the wild ...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
...erent dairy animals like buffalo, cow, sheep, goat and camel. The buffalo and sheep milks have comparatively higher fat, solid-not-fat and total solid contents than other milks. Maximum whey proteins were found in the sheep milk (0.78%) whereas cow milk had lowest contents (0.54%). Non-casein-nitrogen (NCN) contents in sheep milk were higher followed by camel and buffalo milks. Electrophor...
Azmat Hayat Khan Khattak1, Muhammad Nauman ul Islam1, Muhammad Shakir Khan1, Muhammad Iqbal1, Imtiaz Ali Shah1,Sheeraz Ghani1, Amjad Ali2, Zubina Hameed1, Mohammad Farooque Hassan3 and Tariq Ali1,3,*
...AJC) and eleven Azakheli buffalo calves (ABC) were reared on milk replacer to compare their growth performance and economics. The experiment was performed at Livestock Research and Development Station (LR&DS), Surezai, Peshawar for a period of 12 weeks. The calves were fed with milk replacer (10% of their live body weight) in two equal doses, one in the morning followed by an evening dose, until the end of 8th week. The average birth weight of A...
Shaista Naz1*, Noor Paio Khan1, Naveed Afsar2 and Ashfaq Ahmad Shah3
...t women were involved in buffalo, cattle, goats and sheep rearing with higher possession of small ruminants (goats and sheep), while men were found dominant in large ruminants’ possession (buffalo and cattle). Women’s higher time allocation to livestock management activities depicted their higher level of participation in livestock management was found high as compared to their male counterparts. Women’s pa...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2
... total of 600 cattle and buffalo were selected from three different topographical locations of Punjab on the basis of clinical biomarkers. Blood and mosquito samples were collected. Blood samples were processed through complement fixation test and RT-PCR, while mosquito samples were processed through RT-PCR. Overall prevalence of bovine ephemeral fever (BEF) in indigenous breeds of bovine was 63.33%, lower, than exotic breeds 64.66% which were found to be more...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
...thawed quality of Kundhi buffalo bull semen. After collection ejaculate was brought in Semen Production Unit. Semen was evaluated as fresh for volume, colour, motility, morphology, live dead ratio, membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen volume and colour were determined by visual examination,...
M. Tariq Javed1, Nazir Ahmad2, Gulbeen Saleem3, Haleema Sadia4, Farkhanda Manzoor5 and Ghulam Hussain Dilbar
EFFECT OF AGE AND SEASON ON SEMEN CHARACTERISTICS OF BUFFALO BULL - A REVIEW
...s of semen parameters in buffalo bull at different age and in different seasons has been published with conflicting reports. An effort is made to review the literature gathered from different sources on semen characteristics in buffalo bulls which will be helpful to researchers and allied professionals in future research or policy planning. The review is bifurcated in different sections for easy understanding of the facts. T...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
...014 to 2015, twenty five buffalos ranging in age between 7 to 9 years, were referred to surgery clinic at University of Veterinary and Animal Sciences, Lahore, Pakistan. The patients showed the signs of ocular lesions associated with plaque formation on the cornea and sclera and papilloma formation on the lateral limbus of eye. The tumors were large and usually protruded through the palpebral fissure and infiltrate the entire eye ball. Diagnosis was made based...
Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2
...ous cattle, sheep, deer, buffalo and goat, etc. Various synthetic anthelmintics have been used, on wide scale, to control the helminths infections.But the parasite worms have developed multiple resistances against the synthetic anthelmintics. Hence, various biological controls, vaccines and local medicinal plants are being explored to control the parasitic worms. The aim of this study was to evaluate the anthelmintic potential of two local plants, Albi...
Rafiullah1*, Abdur Rahman2, Khalid Khan1, Anwar Ali1, Arifullah Khan2, Abdul Sajid3 and Naimatullah Khan3
...imal species (cattle and buffalo) were selected. A total of 800 samples were collected from different areas of District Lakki Marwat and District Peshawar. All the samples were processed through different diagnostic techniques, including Microscopy, ELISA and Real-time PCR for diagnosis of Theileria parva at Veterinary Research Institute Peshawar. The overall prevalence for Theileriosis was 27.75% through Real-time PCR, followed by Indirect ELISA (24.62%) and ...
Abdul Wahaab1, Muhammad Ijaz1,*, Syed Saleem Ahmad1, Umair Iqbal2, Abdul Tawaab1 and Iahtasham Khan3
...ated bovines (cattle and buffaloes) and their effect on various blood parameters (WBC, RBC, Hb, HCT and MCHC). For this purpose, 40 infected animals were selected. After dividing them randomly in four groups of 10 animals each, drug was administered to first three groups whereas last one was kept under positive control for comparison. Study revealed that Triclabendazole was most effective drug of all three in both cattle and buffalo
Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1
... collected from cows and buffaloes kept at different localities in district Kasur, Punjab, Pakistan. The possible risk factors inquired from farmers were animal type, breeds, urbanicity, age (years), teat washing, bedding area, lactating stage and previous exposure of mastitis. Initially surf field mastitis test (SFMT) was applied for screening subclinical mastitis followed by bacteriological techniques on positive milk samples for confirmatory isolation of
Fazal Abbas1, Muhammad Ijaz1,*, Zunaira Akhtar1, Khalid Mehmood2, Muhammad Zeeshan Hyder3 and Umair Iqbal4
...n=60 cattle calves; n=60 buffalo calves) under one month of age irrespective of cause of diarrhea was selected from different livestock farms located in the outskirts of Lahore. The result showed that there was increased in packed cell volume (%) in their blood profile. The sodium and potassium concentration in ppm significantly increased (P< 0.001) while the calcium, copper and iron concentration in ppm significantly decreased (P< 0.001) in the calves h...
Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3
... mastitis in a cohort of buffaloes at Khyber Pakhtunkhwa. 120 quarter samples were collected from suspected buffaloes in selected areas of Peshawar, Charsadda, Mohmand Agency, and Dara Adam Khel. Initially, California Mastitis Test was performed for screening of positive samples. Afterward, the bacterial profile was confirmed through biochemical testing. The quarter wise prevalence of subclinical mastitis was 25%. Within thi...
Asghar Khan1,*, Aneela Zameer Durrani1, Arfan Yousaf2, Jawaria Ali Khan1, Mamoona Chaudhry1, Mumtaz Ali Khan3, Habibunnabi4 and Amjad Khan5
...al mastitis in lactating buffaloes in Pothohar region of Pakistan. A questionnaire based cross-sectional based survey was conducted in 30 commercial and subsistent farms in Pothohar region of Rawalpindi district, Punjab. Where randomly 196 lactating buffaloes were sampled. Data on several health, management and bio-security variables was collected in face to face interviews from farmers, managers and owners. Milk samples col...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
... and compare the role of buffalo oviductal epithelial cell (BOEC) co-culture and conditioned culture media, on in vitro development of Nili Ravi buffalo embryos. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertiliza...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
...dy, BMP15 gene of buffalo was cloned, analyzed, and its’ expression pattern was further determined. It consists of 1185 nucleotides that encodes 394 peptides bond. The BMP15 gene was observed to be expressed in cumulus tissue, granular tissue, hypophysis, genital ridge and tissues of ovary and testis. Moreover, QRT-PCR results showed that BMP15 was expressed in the whole process of embryogenesis and folliculogenesis, early high level...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...ic disease of cattle and buffaloes, causing progressive weight loss, persistent diarrhea and finally death. Due to zoonotic nature of the disease, workers in slaughterhouses are at a high risk of infection. In this study, the status of paratuberculosis was assessed in a slaughterhouse located in district Faisalabad, Pakistan. A total of 455 blood samples were collected from slaughtered cattle and buffaloes at random and then...
Muhammad Abdul Rahman1*, Abdul Saboor1, Gulnaz Hameed1, Ghulam Bilal2 and Farooq Tanwir3
...seases, sensitiveness of buffaloes to temperature, slow and reduced weight gain and effect on birth and death rate are weighted high as threat to farm animal health while India-Pakistan ties on water, water scarcity, high water requirement for buffaloes and lack of innovation in processing of milk and milk products are other major threats to production from farm animals. The findings of this study can serve as basis of provi...
Doulat Khan1, Hamayun Khan1, Nazir Ahmad2, Muhammad Tarique Tunio3, Muhammad Tahir2, Muhammad Saleem Khan2 and Rifat Ullah Khan1*
...dentification in cattle, buffalo, goats and sheep. A total of 120 blood were taken from jugular vein of different breeds of cattle (Achai, Achai x Jersey, Holstein Friesian and Jersey), buffalo (Nilli Ravi, Aza Kheli and non-descript), goats (Beetal, Teddy and non-descript) and sheep (Bulkhi, Karri and non-descript). In cattle, the average sensitivity, specificity, false pregnancy prognostic value, false non-pregnancy progno...
Savas Atasever*, Huseyin Erdem and Ibrahim Cihangir Okuyucu
...men fill scores (RFS) in buffaloes. In total, 66 Anatolian buffaloes reared in four farms of the Middle Black Sea region of Turkey were scored subjectively by two parameters. BCS and RFS data were obtained using a 1 to 5 scale and a 1 to 4 scale, respectively, where lower points indicate thin body condition or relatively empty rumen. Animals were examined in two subgroups by parity (1= lactation number 1 and 2; and 2= l...
Veli Sel1, Isa Yilmaz2,* and Mete Yanar3
...farms of Anatolian water buffalos in Igdir. For this purpose, a total of 637 milk samples gathered from 91 animals raised in 54 farms were analyzed and statistically evaluated. The age and education level of the farmers and the effect of the milker on the SCC were found to be significant (P <0.001). SCC value (65.930 ± 2.484 cells/ml) was found to be the lowest when the milker is the person from the household. The udder cleaning before milking was a ...
Saeed Murtaza1, Abdul Sattar1*, Nasim Ahmad1, Muhammad Ijaz2, Maqsood Akhtar3 and Muhammad Shahzad4
...oxytocin in newly calved buffalo has made buffalo breeder anxious about its reproductive and productive performances. Moreover, misconceptions about its harmful effect on public health are increasing day by day during postpartum period when animal has been already in stress. To know effect of oxytocin injection on cervix, uterus and milk composition during postpartum interval in Nili-Ravi buffalo
Ertuğrul Kul*
...) records from Anatolian buffaloes calved during 2014-2017 raised under different farm conditions in Amasya Province, Turkey. Five different lactation curve models such as Wood (WOD), Cobby and Le Du (CLD), Exponential (EXP), Parabolic Exponential (PEXP) and Quadratic (QUA) models were used. The adjusted multiple coefficient of determination (R2adj) and the residual standard deviation (RSD) were evaluated as parameters to detect th...
Hifzulrahman1, Muhammad Abdullah1*, Muhammad Uzair Akhtar2, Jalees Ahmed Bhatti3, Muhammad Saadullah1 and Muhammad Naveed-ul-Haque2
...arly-lactating Nili Ravi buffaloes. Sixteen early-lactating Nili Ravi buffaloes received 2 treatments in a completely randomized design with a period length of 120-d. The treatments were 1) Ctrl = basal diet without supplementation of fat, 2) Ca-FA = basal diet with calcium salts of palm fatty acids supplementation. The Ca-FA treatment increased the 3.5% fat-corrected milk yield by 11.0% compared with the Ctrl, whereas, the ...
Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan2
...tating indigenous cattle/buffaloes raised on different commercial dairy farms and exposing the hazards linked with milk-born tuberculosis infection in the area where unpasteurized milk is normally used in the central zone of Khyber Pakhtunkhwa (KP), Pakistan. A total of 1225 cattle and 1175 buffaloes of more than one year were studied for the prevalence of bovine tuberculosis. A total of 1608 milk samples (793 from cattle an...
Tingting Li, Shuangshuang Geng, Long Xie, Huiyan Xu, Aolin Luo, Pengwei Zhao, Huan Yang, XingWei Liang, YangQing Lu, XiaoGan Yang* and KeHuan Lu*
...nswer this question, the buffalo GDNF gene was cloned and integrated into the PiggyBac eukaryotic expression vector, which was stably expressed in Sertoli cells, thus establishing a PB-GDNF-Sertoli cell line. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that the GDNF gene expression in the PB-GDNF-Sertoli cell line was significantly increased compared with that in the control Sertoli cell line (p<0.01). Enzyme-linked immunosorbe...
Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2
A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
...the households possessed buffaloes. Average number of buffaloes was estimated at 4.28 while 376 (63%) of the household reported cattle with average number of 4.38 heads per household. Small numbers of buffalo and cattle bulls were recorded. Average number of 5.55 goats per households was reported by 35% of all the households. On an overall basis, 2,365 liters of bu...
Shahid Ali*, Aijaz Ali Channa, Waqar Ahmad, Rafia Akram and Imbasat Yousaf
...rm pluriparous Nili Ravi buffalo (Bubalus bubalis) that was recumbent and under extreme stress because of intemperate bilateral abdominal distension. Trans-rectal palpation revealed a taut uterus (bulged, tense and inelastic) and alive fetus. Subsequently upon induced cervical dilatation, 60-70 L of amniotic liquid was expelled out and dam delivered completely developed alive male calf with a gross anomaly (presence of a hole in the skull) which could n...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...reservation of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different ...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...rtility of cryopreserved buffalo spermatozoa by addition of arachidic acid in semen extender. For this purpose, semen was collected from 3 adult buffalo bulls (Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temper...
Muhammad Aamir Naseer1, Amjad Islam Aqib2*, Ambreen Ashar3, Muhammad Ijaz Saleem1, Muhammad Shoaib4, Muhammad Fakhar-e-Alam Kulyar1, Khurram Ashfaq1, Zeeshan Ahmad Bhutta1 and Shagufta Nighat5
...) from cattle (n=90) and buffalo (n=160) were aseptically collected from various dairy farms and put to biofilm detection and antibiogram. Based on collected data with statistical inferences, the study found 61.60% of S. aureus from subclinical samples, while 72.73% of S. aureus were positive for biofilm with uniform hike in samples from cattle (77.55% bpSA) and buffalo (70.48% bpSA). Udder condition/consistenc...
Mudussar Nawaz1, Iahtasham Khan2, Muhammad Shakeell*, Arfan Yousaf1, Zahid Naseer1, Munibullah1, Ali Zohaib3, Riaz Hussain4 and Qudrat Ullah5
...alence of brucellosis in buffaloes and goats of Islamabad Capital Territory, Pakistan. A total of 341 milk samples from buffaloes (n=180) and goats (n=161) were screened by (Milk Ring Test) MRT and milk i-ELISA for anti-Brucella antibodies. Higher prevalence was found in buffaloes (5.6% and 16.1%) than in goat (4.97% and 1.9%) both through MRT and i-ELISA respectively. Commercial pr...
Masoud Hassani1* and Omid Madadgar2,3
Serological Evidence of Bluetongue in Iran: A Meta-Analysis Study
...ne, cow, cattle, bovine, buffalo, camel, Iran and prevalence alone or in combination in both English and Farsi language. After reviewing 82 published articles, a total of 48 studies from 29 articles were eligible to be included in this meta-analysis study. The total seroprevalence of bluetongue in apparently healthy sheep, goat, cow and camel at animal level based on ELISA test was 50.4% (95% CI= 43.5–57.2), 79.2% (95% CI= 70.7–85.8), 3.3% (95% CI=...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2, Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5, Hamayun Khan1 and Shabana Naz6*
Said Sajjad Ali Shah1*, Muhammad Ilyas Khan1, Aziz Ullah1, Hayat Ullah2 and Faisal Ahmad2
Coprological Examination of Small and Large Ruminants in Central Zone of Khyber Pakhtunkhwa
... 51.5% in cows, 58.3% in buffalo, 73.9 % in sheep and 68.5% in goats. Trichostrongylus (32.8%) was recorded as highly prevalent in cattle followed by amoeba (10.2%) and Fasciola (6.1%), while in buffaloes fasciola was recorded higher followed by amoeba (19%) and Trichostongylus (5.35%). Haemonchus contortus was the most prevalent intestinal parasite in the study area followed by Trichostrongylus in small ruminants, whereas m...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...rowth characteristics of buffalo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then enriched with a differential plating method. Isolated SSCs were cultured on STO feeder cells with SSCs culture medium (SSCM), which is serum-free. ...
Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3
...horses, donkeys, cattle, buffalo, sheep, goats, pigs, chickens, geese, turkeys, ducks, guinea fowls, dogs and bees were analyzed. The number of populations these indigenous breeds in Serbia has been shown in this paper. It is proposed that 15 breeds (13 breeds and 2 strains), which are not protected by the Decree of the Ministry of Agriculture of Serbia on animal genetic resources, be included in the Decree in the future, in order to preserve and improve their...
Tanveer Hussain, Masroor Ellahi Babar*, Akhtar Ali and Fiaz Hussain
...estic goat, sheep, water buffalo and cattle for biological positioning of dromedary camel. The results reconfirmed the classical biological classification.
...
Eman Khalifa1*, Riad Khalil2 and Haitham Elaadli3
...bacterium bovis from buffaloes’ mastitic milk by isolation on Lowenstein-Jensen medium (LJ medium), Ziehl-Neelsen staining; identification by different biochemical tests and polymerase chain reaction (PCR) at 500bp diagnostic for M. bovis, as well as (2) to determine the therapeutic efficacy of the various antimicrobials (9 types) on the isolated M. bovis by using different antimicrobial sensitivity plate method. A total number of 100 s...
Yongyun Zhang1, 2, Xinyang Fan1, Fangting Zhou1, Weizhen Li3, Yina Ouyang1,4 and Yongwang Miao1*
...been fully understood in buffalo. In this study, the polymorphisms in coding sequence (CDS) of the CSN1S1 gene for river and swamp buffalo were detected using PCR product direct sequencing. The CDS for both types of buffalo was the same in length, which contained 645 nucleotides and encoded a peptide composing of 214 residues. A total of 5 single nucleotide polymorphisms (SNPs) was ...
Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*
...iochemistry in Nili Ravi buffaloes under subtropics is lacking. This study was designed to fulfill this gap by analyzing the effects of exposure to the bacterial culture of Pasturella multocida (P. multocida) type B:2 and its immunogens, i.e., lipopolysaccharide (LPS) and outer membrane protein (OMP) on Nili Ravi buffaloes. Prepubertal Nili Ravi female buffaloes (N=18) of 10-12 months age ...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...s were due to cattle and buffalo differences. Phylogenetic analysis and evolutionary divergence were also estimated. The sequence was submitted to GenBank (NCBI) with accession number MN712202. Our results represent a preliminary step towards the identification of polymorphisms in STAT5A gene of Nili Ravi Buffalo. Further studies are required for the association of genetic polymorphisms of STAT5A gene with fert...
Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2
...alves of cattle (32) and buffaloes (8) below three months in some farms in Egypt. The detection was done by ELISA and indirect fluorescent antibody technique (FAT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal diarrhea. Six out of forty tested samples were found positive for rotavirus (15%) infection using ELISA test. Successful isolation of the rotavirus on MDBK cell line from ...
El-Bagoury, G.F., El-Nahas, E.M., El-Habbaa, A.S.
...ally infected cattle and buffaloes. Twenty Buffy coat samples were collected from each clinically infected cattle and buffaloes in Qaluobia province during the year 2014. The virus was isolated on BHK-21 cell line. Infected cells with BEFV showed 80% cytopathic effect (CPE) after 48 h by the 3rd virus passage. The isolates were serologically identified by neutralization and immunoflourescence techniques, and then molecularly...
Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3
...om vaccinated cattle and buffalo from five Egyptian
governorates (Two governorates represent Delta region: El-Gharbia – Kafr El Sheikh , Two
governorates represent upper Egypt: El-Fayoum – El-Menya , and one governorate represents
central : El-Giza). The sera collected from diseased and apparent healthy animals. Results of
Serum Neutralization Test were 260 of cattle sera out of 280 and 167 of
Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3
...fecting cattle and water buffaloes. The present work aimed to isolate the causative agent and identify it by serological characterize of the viral antigens, then molecular characterization of its genome. Infection by the bovine ephemeral fever virus (BEFV) was noticed in Dakahlyia governorates during the summer season of 2015. Twelve samples of buffy coats were collected from dairy farms suspected from clinical investigations to be infected by bovine ephemeral...
Muhammad G. Khodary1, Ayman H. El-Deeb2, Mohamed M. Emara2, Othman E. Othman1, Hussein A. Hussein2
...tbreaks among cattle and buffalo herds in Egypt. This study aimed to investigate the
contribution of Foot and Mouth Disease Virus (FMDV) and characterization of the circulating strains
associated with the outbreaks.
Methods: The collected samples of Oropharyngeal, oral epithelial tissue and/or vesicular fluids were
tested by real time Reverse transcriptase polymerase chain reaction (rRT-PCR) for detection of
Sami Ullah1, Bernhard Brümmer2 and Umar Ijaz Ahmed1*
...ved crossbred cattle and buffalos to ensure good quality breeds....
Usman Asghar1*, Muhammad Faheem Malik1, Umer Rashid2, Naeem Mahmood Ashraf2, Sumera Afsheen1 and Muhammad Hashim1
...m muscle tissues of cow, buffalo, goat, donkey and a dog. A set of degenerative primers was designed for Polymerase Chain Reaction (PCR) of the cytochrome b gene fragment. All PCR was accomplished at the same reaction conditions. Amplified products had a length of 400 bp, which was our estimated length. After the sequencing of these amplicons, In silico restriction analysis was performed for each specie. Restriction endonuclease TfiI was selected for further a...
Nasir Ali Tauqir1, Asim Faraz2*, Muhammad Arif1, Abdul Rehman1, Imtiaz Hussain1, Abdul Waheed2, Gabriele Marino3 and Michela Pugliese3
...y to lactating Nili-Ravi buffaloes on their nutrient’s intake and utilization, milk yield and composition, nitrogen balance and hematological parameters. Fifteen lactating Nili-Ravi buffaloes in mid lactation with almost similar milk production were randomly divided into three different groups of five females within each according to completely randomized design. Three iso-caloric and iso-nitrogenous diets with high, m...
Muhammad Anwarullah1, Aneela Zameer Durrani1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Usman1*,Muhammad Zahid Iqbal1, Muhammad Husnain1,Ahsan Anjum3, Qurratulain Ashraf4 and Ghulam Mustafa3
... (MAP) in cattle and buffalo. Diarrhea is a major risk factor for MAP, so 1100 blood samples were collected from diarrheic animals or having history of some diarrheic episodes from four districts of Punjab i.e. Kasur (n=300), Rawalpindi (n=230), Lahore (n=230) and Bahawalpur (n=340). These samples were tested using ELISA and overall seroprevalence found was 39.64%. The major host-associated and non-associated risk factors of MAP were also considered. It wa...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...ity indicators of frozen buffalo bull’s spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen buffalo ...
Amtul Jamil Sami*, Sehrish Bilal and Syeda Anum Zahra
...riations in the genes of buffalo GH and its possible effects in milk production. The present research was carried out to explore DNA polymorphism in buffalo growth hormone gene. In this study we amplified a 5’ flanking region covering exon 1 from local specie of Bubalus bubalis. As the 5’ region of the GH is very important in controlling the expression of the gene and minor changes in this sequence can affect its...
Bikash Puri1, Anil K. Tiwary2, Bharata Regmi3,4, Dinesh K. Singh1, Doj R. Khanal5 and Manoj K. Shah3*
...althy ruminants (cattle, buffalo, sheep and goat) and screened for Bluetongue virus (BTV) antibodies in sera using a cELISA kit. Out of 220 sera samples, 92 were positive for BTV, accounting for 41.8% prevalence in ruminants. Seroprevalence rate was the highest in Buffaloes (58.3%) followed by sheep and goats (each 40%), and cattle (37.5%). The BTV seropositivity varied significantly (p<0.001) among ruminants in different...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
... (n=4) healthy Nili Ravi buffalo bulls was collected through artificial vagina and Gallic acid was added to the semen at 1 µM, 15 µM, 30 µM, 45 µM, 60 µM, and 100 µM. A total of six groups were prepared. One group was kept as control where no Gallic acid was added. After the addition of extender, semen was cooled to 4°C, filled in 0.5mL straws for 4 h and frozen in liquid nitrogen at -196 °C. The parameters evaluated...
Sharjeel Saif1, Asghar Ali Kamboh1*, Ghulam Mustafa Solangi2, Rehana Burriro3, Hasina Baloch1, Atta Muhammad Memon2
... from mastitis suspected buffaloes. The samples were collected from those animals which had abnormal milk or physical signs (like swelling, abscess, wound etc.) on udder or teats or have suddenly dropped milk production. All samples were cultured on PPLO (pleuropneumonia like organism) media and were found negative for Mycoplasma spp. The prevalence of mycoplasma mastitis in this population of dairy buffaloes was recorded &l...
Sonali Menamvar1,2,3, Kirubakaran Vinod Kumar1, Veeregowda Muniveerappa Belamaranahally2, Yella Narasimha Reddy3, Rathnamma Doddamane2, Shrikrishna Isloor2, Ramesh Poojari Thimmaiah2, Gurrappanaidu Govindaraj1, Bibek Ranjan Shome1, Vinayagamurthy Balamurugan1*
...seases of the cattle and buffaloes population worldwide and is caused by serovars of pathogenic Leptospira. A pilot study was conducted in some of the dairy farms located in Telangana and Andhra Pradesh, endemic states of India, during 2017 to investigate the seropositivity of bovine leptospirosis in dairy animals and associated risk factors at the farm level. The semi-structured questionnaire was designed as per the literature and used to collect leptospirosi...
Mohamed Abd El-Fattah Abo-Farw1, Maged Ahmed Aboul-Omran1, Abdel-Khalek El-Sayed Abdel-Khalek2*
...ace elements in Egyptian buffaloes. A total of 90 multiparous lactating Egyptian buffaloes (500-600 kg live body weight, 5-7 years old, 3-5 parities, and 10.75 ± 2.66 kg/day milk yield were used in this study. The buffaloes were managed and feed at similar traditional conditions. Mastitis diagnosis was performed 21 days after calving, then animals (n=90) were grouped to normal (n= 7...
Hamdy Abdala Elnagar*, Wael Mohamed Wafa, Moataz Ibrahim Badwy, Abdelaziz Mustafa Sakr
... in low and high fertile buffalo-bulls. Also, the relationship between protein profile in BP of adult bulls and bull calves were studied. Blood samples were taken from 3 calves (128.33±7.58 kg and ageing 6 mo.) and 10 bulls (400±37.5 kg and 24-25 months of age) and semen samples from bulls. Semen was collected once a week from all bulls which were divided according to semen quality to high and low fertile bulls (5 for each). Reaction time and sem...
Muhammad Zahid Iqbal1, Aneela Zameer Durrani1, Jawaria Ali Khan1, Nisar Ahmad2, Muhammad Usman1,*, Abdul Jabbar1, Saba Usman3, Ahsan Anjum3, Muhammad Husnain1, Nadeem Raza1 and Anwar-ul-Haq4
...2% and 12.5% in cows and buffaloes, respectively. The association of selected clinical biomarkers was also ascertained and all of these were found significantly (p<0.05) associated with the incidence of coxiellosis. The results of animal-related risk factors indicated that with increasing age and parity number, poor BCS, or a history of disease, the incidence of coxiellosis became significantly (p<0.05) higher. Various farm-related risk factors were also...
Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3*
...uality of blastocysts in buffalo through inhibition of glycogen synthase kinase 3. The purpose of this study was to increase the blastocyst rate and quality of parthenogenetic embryos as a source of pESC primary colonies. Fertilized embryos as positive control group. Diploid parthenogenetic embryos were divided into three treatment groups: CHIR99021 0 mmol/l, CHIR99021 0.003 mmol/l, and CHIR99021 0.01 mmol/l. The embryonic stem cell (ESC) primary colonies were...
Muhammad Ali Raza1*, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Kamran Ashraf2, Muhammad Muddassir Ali3, Kumayl Hassan Akhtar4 and Nazia Rubab5
...ck products like milk of buffalo and cow. It leads to antibiotic resistance in human beings as well as animals and thus lead to the problem for one health (OH). Following were the objectives of the current study. Detection of commonly used antibiotics (penicillin and oxytetracycline) in (i) raw milk of buffalo and cow collected open market, (ii) UHT milk, (iii) pasteurized milk, (iv) bulk tank milk and (v) gawala’s (mi...
Manish Gautam1*, Pravakar Kattel2 and Krishna Kaphle3
...ainly affects cattle and buffalo manifesting nodules in skin and mucous membrane with a high fever. Since the first report of this disease from the Morang district of Nepal in 2020, the disease has been spreading in various parts of the country. Cattle are comparatively more affected in Nepal than buffalo. Though this disease has less mortality rate it causes great economic losses by decreasing milk production, abortion, inf...
Wafaa M.A. Ghoneem*, Muhammad M. Shaklouf, Ali M. Ali and Mohammed H. Bakr
...stify;">Thirty lactating buffaloes (650 ±50 kg average body weight, in the first to the fifth seasons of lactation, 127±4 Days in milking and average of 12.32±1.13 kg daily milk yield) were divided into three similar groups to evaluate the effect Lactobacillus acidophilus fermentation product (LAFP) commercially named (Culbac®) on nutrients digestibility, milk yield and composition, blood biochemistry and immune system response of the ...
Sher Bahadar Khan1,*, Mumtaz Ali Khan2, Irshad Ahmad3, Faheem Ahmad Khan4, Hamayun Khan1 and Sher Ali Khan5
...f 1250 milk samples from buffaloes were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes through PCR. Among them, the highest prevalence of Staph. aureus was found in Peshawar-Mardan division (30%), followed by Malakand (28.5%), Bannu-Dera Ismail khan division (25%) and Hazara division (16%). Over all the high resistance was found against Lin (96.25%) followed by AMX (82.5%), TET (63....
Hamdy Abdala Elnagar, Wael Mohamed Wafa*, Abdelaziz Mustafa Sakr, Mohammed Mahmoud Hegazy
...ge advancing of Egyptian buffalo males from one up to 24 month of age. Blood samples were taken from 66 male buffalo calves at 1, 3, 6, 12, 18 and 24 month of age (11 animals at every age). TES, leptin and PRL levels were determined in blood plasma. Results showed insignificant increase in TES and PRL by age advancing at 1-3 month, and in leptin at 1-6 month of age. All hormones markedly increased up to 24 month of age. TES ...
Muhammad Zaeem Abbas1, Khurram Ashfaq1, Amjad Islam Aqib2, Mughees Aizaz Alvi1*, Muhammad Haleem Tayyab1, Muzafar Ghafoor1, Muhammad Usman Naseer3, Ali Abbas4 and Ali Hassan1
...ed risk factors in dairy buffaloes. In this regard, milk and urine samples were individually collected from 96 buffaloes and analysed by Ross Modification of Rothera’s test and Abbott Precision Xtra™ meter to assess the ketone bodies levels. We observed a total of eight out of 96 (8.33%) buffaloes positive for ketosis. The peak prevalence (13.3%) of ketosis was detected during ...
Deepesh Kumar Bhuptani1, Atta Hussain Shah1*, Gul Bahar Khaskheli1, Zubair Ahmed Laghari2, Ghulam Shabir Bahram1, Muneer Ahmed Jamali1, Tarique Ahmed Khokhar1
...ification of AFM1 in raw buffalo milk in Sindh province. A total of three hundred and fifteen raw milk samples (n=315) from dairy farms of three regions in Sindh province i.e., southern (Karachi, Thatta, Hyderabad), central (Mirpurkhas, Umerkot, Shaheed Benazirabad) and northern (Naushahro Feroze, Sukkur and Larkana) were collected and analyzed using commercial ELISA kit. The results revealed that AFM1 was statistically higher (P˂0.05) in district Karachi (0....
Ngoc Tan Nguyen1*, Pham Kim Ngan Nguyen1, Thao Huong Phan1, Tan Loi Le1, Thi Khanh Ly Nguyen1, Tuan Thanh Hoang2, Cong Thieu Pham3, Cong Dinh Nguyen3, Nguyen Khang Duong4
...etic diversity of native buffalo populations in different regions of Vietnam based on nucleotide sequence in a displacement loop (D-loop) of mitochondrial DNA (mtDNA). Thirty-one blood samples that were collected from three native buffalo populations such as Bao Yen (BY: 7 samples), LangBiang (LB: 7 samples) and Thanh Chuong (TC: 7 samples) and two imported buffaloes from Thailand (T: 5 sa...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
...or sperm preservation in buffalo bulls at animal production research institute, Sakha, Kafr El-Sheikh Governorate, Egypt. The experimental protocol was designed as four groups (Control, G1, G2 and G3). Buffalo-bulls’ semen were exposed to different concentrations of garlic extract (0, 3, 6 and 9%, respectively) to evaluate the semen quality parameters after cooling and freezing process. Data obtained showed that, progr...
Tariq Ali1,2*, Kamran1, Abdur Raziq1, Inam Ullah Wazir1, Anwar Ali1, Muhammad Ijaz Ali1, Muhammad Shuaib Khan2, Shakeeb Ullah2 and Sher Hayat Khan3
...ases in dairy cattle and buffaloes. This study describes the current prevalence of mastitis in lactating cattle, and buffaloes in Khyber Pakhtunkhwa during 2019-2020, to identify bacterial pathogens of mastitis, and to determine the antimicrobial resistance. A total of 1013 milk samples belonging to 726 cattle and 287 buffaloes were examined to detect clinical mastitis (CM) and subclinical...
Hurriya Alzahra1, Susmiati Susmiati2*, Sri Melia3
Juweria Abid1, Shakeel Ahmad2, Humaira Wasila3, Tooba Asif4, Muhammad Farooq5* and Attaullah Jan6
... activity of fresh (cow, buffalo, goat, sheep and camel) and processed milk types (S1, S2, S3, S4 and S5) commonly consumed in Khyber Pakhtunkhwa (KP), Pakistan. Samples were collected from three different districts of KP and after composite sampling dried in oven and stored at room temperature for further biochemical analysis. Samples were then analyzed for minerals content, total phenolic compounds, total flavonoids and antioxidant activity. Physicochemical ...
Lobna M.A. Salem, Nashwa O. Khalifa, Marwa O. Abd El-Halim*
...infecting sheep, cattle, buffaloes, goats, and camels in Qualyobia, Egypt. The genetic identity of Fasciola species was examined by the analysis of forward and reverse sequences of the ITS2 of the rDNA gene that amplified in 300bp. Out of 286 slaughtered animals {88 sheep, 26 goats, 68 cattle, 25 buffaloes and 79 camels} in the regions of Qualyobia, 51 (17.8%) had Fasciola spp. morphologically {27/51(52.9%) were identical to...
Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1*
...estrus cycle in Egyptian buffaloes. Hence, the study of molecular control of the CL in Egyptian buffaloes let us to know the mechanisms of this regulation. We aimed to (1) figure out the expression profile of apoptotic genes (TNFα- BAX - CASP3 - FASLG - AGTR2 - and NOS2) mRNAs during early, mid, and late stages of CL and (2) clarify the accompanied changes in progesterone (P4), nitric oxide (NO) concentrations and hist...
Sherif Abdelghany1, Hossam Mahrous Ebeid2*, Ahmed Abdelkader Aboamer2, Mohamed Ali Radwan1, Rania Agamy1
...crossbred cows and local buffaloes in mid-lactation, with an average of 4.4 parties and an average herd size of 1.28 and 1.19 head/farm for Nile Valley and Newly Reclaimed districts, respectively. A structured questionnaire survey was designed, and field interviews were conducted to collect data and to characterize the current situation. Representative feed staff and milk samples were collected and analyzed. Feeding practices efficiency indicators were calcula...
Iqra Qazi1, Sohail Raza1*, Masood Rabbani1 and Muhammad Avais2
...sitivity was observed in buffalo (73.12%) as compared to cattle (60%) moreover, the sero-prevalence is higher in animals of greater size herds. Parity status is in agreement of strong correlation with BoHV-1 infection. Evidently, positivity rate was higher in animals bred with artificial insemination as compare to natural breeding method....
Aqsa Batool1, Masood Rabbani1*, Farhat Nazir Awan2, Sohail Raza1, Sehrish Firyal3 and Shahan Azeem1
...ollected from cattle and buffalo of wheat rice Punjab, mixed Punjab, cotton/wheat Punjab, Low intensity Punjab and Rain-fed Punjab zones. The study revealed individual seroprevalence of BoHV-1 49.5% in cattle and 51.5% in buffalo. Over all prevalence in five zones of Punjab was observed as 25% in mixed Punjab to 71.25% in Rain-fed Punjab. The BoHV-1 seropositivity ranged from 30 - 60% in cattle and 20-75% in
Neni Widaningsih1,2*, Budi Hartono2, Hari Dwi Utami2, Eni Siti Rohaeni3
... transformation of swamp buffalo (Bubalus bubalis carabauesis) with digital-based marketing. This research has been carried out from January to June 2022 in Paminggir District, Hulu Sungai Regency, North of South Kalimantan Province. The methods used in this study are survey and observation methods. Data collection carried out includes; purposive sampling and accidental sampling techniques with the main respondents, namely breeders, collecting merchants, whole...
Kamel Etman Ibrahim Etman*, Salah Kamal Sayed, Farouk Amin El-Sayed, Abd-Alghany Hasaneen Ghoniem, Mostafa Mohamed El-Nahrawy, Mona Ahmed Alsayed Farag
...s with using thirty male buffalo calves in fattening trial. Rumen and blood parameters were also studied. First group (10 animals) were received control ration which contains 60 % concentrate feed mixture (CFM), 30 % corn silage (CS) and 10 % alfalfa hay (AH). The 2nd and 3rd animal groups were fed control ration supplemented with 5 gm probiotics bacteria and 10 gm fibrolytic enzyme per head , respectively. The experiment lasted about 180 days. Animals fed r...
Ahmed Fathy1, Rania A. Hassan2, Essam S. Soliman3*
...ification between Baladi buffaloes and crossbred using clinical epidemiological measures of some productive and reproductive traits such as milk yield (MY/kg), days in milk (DIM/days), age at first calving (AFC/months), calving interval (CI/months), days open (DO/days), dry period (DP/weeks), number of services per conception (SPC rate), and days to first insemination (DFI/days). The raw data on 927 buffaloes (763 crossbred ...
Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza
Mian Shamas Murtaza1,*, Aysha Sameen2, Saima Rafique3, Muhammad Shahbaz1,*, Nabeela Gulzar4, Mian Anjum Murtaza5, Umar Farooq1 and Iram Hafiz6
Muhammad Iqbal Anjum*, Shahbaz Javaid, Agha Waqar Yunus, Faisal Ashfaq and Javed Iqbal
...l Mixed Ration (TMR) for buffalo calves, 18 calves (aged 14±2 months weighing 189±15 kg) were randomly divided into six equal groups. Six TMRs [iso-nutritious with 12% CP and 68% TDN] having crop residues MS, MC and WS each supplemented with 75% and 65% concentrate (C) were formulated as: T1) MS to C ratio of 25:75%, T2) MS to C ratio of 35:65%, T3) MC to C ratio of 25:75%, T4) MC to C ratio of 35:65%, T5) WS to C ratio of 25:75% and T6) WS to C ...
Sutawi*, Ahmad Wahyudi, Abdul Malik, Suyatno, Asmah Hidayati, Imbang D. Rahayu, Endang S. Hartatie
... of boneless frozen beef/buffalo meat originating from India continued to increase from 33.8% in 2016 to 51.9% in 2020. Third, economic bioterrorism, an act of terror using biological agents that infects Indonesia’s cattle farming agribusiness. FMD outbreaks have a negative impact on cattle agribusiness from upstream to downstream: (1) the cattle population will shrink, (2) imports of cattle and beef will increase, (3) beef consumption will decrease, (4)...
Hussein Jabar Jasim1*, Amer Murhum Al-Amery2
... changes in the infected buffaloes with fasciolosis. Also, the current study recognized the gross and histopathological changes resulting from the invasive Fasciola spp. in the liver and gallbladder. The biochemical results showed statistically significant increases in the serum total bilirubin, creatinine, and active serum enzymes ALT, AST, ALP, and GGT. In contrast, the serum total albumin showed a significant decrease in infected bu...
Hanaa M. Abdelkhalek1, Hanan E. Nagib1, Randa S. Elias1, Saad S. Mansour1, Walid S. Mousa2*
...in most dairy cattle and buffaloes herds. Pseudomonas aeruginosa (P. aeruginosa) is opportunistic pathogens involved in veterinary disorders including clinical mastitis in buffaloes. This study aimed to investigate the antibiogram pattern and synergistic effect of amikacin and norfloxacin against resistant P. aeruginosa isolates from mastitis origin. In addition, detection of some virulence and antibiotics resistance genes. ...
Muhammad Mohsin Abbas1*, Sajjad Ur Rahman1, Muhammad Abubakar2, Muhammad Imran Arshad1 and Khurram Ashfaq3
...ith (60.55%) compared to buffalo with (36.58%). It was concluded that serotype O was more prevalent in district Bahawalpur followed by serotype Asia 1 and serotype A and cow population was found more susceptible than buffalo....
Nasir Ali Tauqir1*, Asim Faraz2, Abdul Waheed2, Ayman Balla Mustafa3, Irfan Shahzad Sheikh4, Michela Pugliese5 and Shahid Nazir6
...n in lactating Nili-Ravi buffaloes. Sixteen early lactating nili-ravi buffaloes were divided into four groups according to Randomized Complete Block Design for this study. Four experimental diets were formulated supplementing 0, 15, 25 and 35g of methionine/ animal/ day. The experimental period was of 56 days out of which 10 days were as adaptation period. Daily feed intake and morning-evening milk yield of each animal was r...
Navjot Hothi
...ainly affects cattle and buffaloes. India has the highest livestock population in the world, and this provides a significant economic contribution to small-scale farmers. The summer and monsoon months of 2022 witnessed a sudden spurge across 14 states and 1 union territory in India, with a spread spanning 251 districts and 43,759 epicenters. Around 2.4 million cattle were affected in India, and 0.11 million cattle succumbed to the disease. The 10 most affected...
Fadzlin Afiqah A. Samad2, Amirul Faiz Mohd Azmi1, Muhamad Affan Ab Azid1, Hafizin Mu’izz Zalazilah1, Syakirah Zulkifli1, Izreen Edriana Mohd Jasmi1, Muhammad Baqir Irfani Rahimin Affandi1, Mohd Zamri Saad1, Md Zuki Abu Bakar1, Agung Irawan3,7, Adib Norma Respati4, Anuraga Jayanegara5,7, Sadarman Sadarman6,7, Hasliza Abu Hassim1,2,7*.
... milk component in dairy buffalo. A dataset comprised of 89 comparisons from multi-species of buffaloes were analyzed according to a linear mixed model methodology with explanatory variables declared as fixed effects and individual study as random effects. The results showed a negative curvilinear pattern of milk yield across buffaloes’ breeds in response to the increasing FC ratio (...
Luigi Liotta1, Vincenzo Lopreiato1, Fariborz Asroosh2 and Alireza Seidavi2*
...s of milk between Talesh buffalo, Talesh sheep, Talesh cow, Talesh-Mediterranean buffalo, Talesh goat and Saanen goat. Milk samples were collected during 2021. The main characteristics and constituents of milk including colour, odour, taste, freezing point, dry matter free of fat, acidity (lactic acid), density (in 15 °C), pH, fat and protein were determined according to the standard methods. Macro and micro elements of ...
Beenish Shahid1* and Muhammad Ijaz Khan2
... of competent oocytes in buffalo species limits the commercialization of in vitro embryo production technology in field conditions. The present study aimed to improve the cytoplasmic and nuclear maturation of in vitro matured oocytes of Nili Ravi buffalo in the presence of hormones. The denuded oocytes (DOs) obtained by repeated pipetting were collected from 2-8mm follicles. A series of experiments were conducted to evaluate...
Sarfraz Ahmad1, Mian Anjum Murtaza1,* and Kashif Akram1,2
...t Mozzarella cheese from buffalo milk. Two hydrocolloids i.e. inulin (0.2, 0.4 and 0.6%) and resistant starch (0.5, 1.0 and 1.5) were added in Mozzarella cheese making from low fat milk (2% fat). The positive (4% milk fat) and negative (2% milk fat) control samples were also prepared for comparison. The Mozzarella cheese samples were analyzed for physico-chemical composition, functional properties, cheese yield and textural profile. The findings showed that th...
Walaa I. Mohameden1, Haidy G. Abdel-Rahman2, Ibrahim M. Hegab3*
...plementation on diseased buffalo calves on health, blood parameters, skin, and behavioral repertoire, and to record the association influencing animals before, during and after minerals supplementation. Twenty buffalo calves suffering from skin lesions were enrolled in this study and the diet was supplemented with mineral mixture for 60 days. Skin conditions and behavioral modifications were monitored alongside blood and pla...
Kinza Javed1, Muhammad Ali1, Waqas Farooq2, Majeeda Rasheed1*, Farkhanda Yasmin3, Samiya Rehman3, Sadaf ul Hira1 and Jawad Ali3
...stemic viral disorder of buffalo and cattle. It is recently discovered disease of cattle which is putting a negative impact on economy of dairy industry in Asia. The rounded Nodules on skin is the early symptom of LSD in clinical investigation. The nodules in the mucous membranes of nose and mouth, subcutaneous edema, emaciation, and exaggerated lymphadenopathy are additional clinical symptoms of this disease. Mortality rates is about 3 % in endemic regions of...
Amirul Faiz Mohd Azmi1,4, Hafandi Ahmad1, Norhariani Mohd Nor1, Goh Yong Meng1, Mohd Zamri Saad2, Md Zuki Abu Bakar1, Norafizah Abdul Rahman5,6, Agung Irawan7,9, Anuraga Jayanegara8,9, Hasliza Abu Hassim1,3,9*
...ypass fat to improve the buffalo performance warrants further investigations, especially in an in-vitro study. Even though several studies have reported the potential of supplementation in enhancing the growth of buffaloes, the effect on a different breeds of buffaloes, the potential to reduce methane production, and the changes in microbial populations remained unclear following different...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...men extender of Egyptian buffalo semen to improve sperm characteristics and antioxidant capacity of sperm medium after cryopreservation. Tris-egg yolk extender with different levels (0, 4, 8, 12, and 14 mg/ml) of propolis ethanolic extract (PEE) were used. Semen was collected once/week for 20 weeks from five buffalo bulls aged 3.5–5 years. The collected semen was pooled and distributed into five equal fractions, each f...
Ishtiaq Ahmed1*, Hamid Ullah2, Zubair Ali2, Muhammad Sohail2, Yasir Amin2 and Afrasyab1
...n goat (80%) followed by buffalo (74%), cattle (71.27%) and sheep (68.08%). The Eimeria spp. was highest (35.27%) followed Buffallo (28.35%) and trichuris spp. 16.41%. Similarly, Ascaris spp. was higher in buffallo (56.71%) followed by cattle (12.1%). Eimeria spp. was highest in Goats (26.41%) followed by sheep (18.75%). The ascaris spp. was higher in goats (13.2 %) followed by sheep (5%), While, Fasciola spp. was higher (15.60%) in sheep than goats (4.70%). T...
Salam N. Aritonang*, Elly Roza, Rizqan, Winda Habsani
...between Swamp and Murrah buffaloes in Humbang Hasundutan Regency, North Sumatra, Indonesia by measurement of body size and body weight. We used 80 buffalo crosses at ≤ 6, 7−12, 13−18, and 19−24 months of age. The research methods used census and survey methods with purposive sampling techniques for determining the research location. We observed variables such as body weight, chest circumference, body len...
Jyotsana Shakkarpude1*, Aditya Mishra2, Deepika D. Caesar2, R.K. Sharma3, Anand Kumar Jain2, Sanju Mandal2, Rajesh Kumar Vandre4, Danveer S. Yadav5, Bhavna Ahirwar6 and C.P. Solanki6
...n appetite of postpartum buffaloes which directly leads negative energy balance (NEBAL) and increases oxidative stress in animals. Betaine is a growth promoting nutritional additive used in livestock. Betaine contains three methyl groups and thus acts as a methyl donor for metabolic reactions. Betaine acts as molecular chaperone, as it repairs heat shock proteins. The present study was aimed to investigate the effect of dietary betaine on heat and metabolic st...
Ferry Lismanto Syaiful1*, Jaswandi Jaswandi2, Mangku Mundana2, Ilham Ilham2, Novirman Jamarun1, Efrizal Efrizal3
...roductive efficiency and buffalo birth. This study is aimed is evaluating the use of mung bean seeds on multiple dosages in the growing seeds tests for the accuracy, sensitivity, and phases of local buffalo pregnancy, and detecting buffalo pregnancy quickly, massively, and cheaper. The object of this study was 40 local buffalo post-AI with the criteria o...
Ghani Khan1, Saeed Ahmed Soomro1, Abdul Kabir2, Abdullah Iqbal3*,Naik Muhammad Marri 4, Syed Ahmad khan5, Muhammad Roidar Khan6 , Anees ur Rehman1, Muhammad Zakir Khan7
...cochemical properties of buffalo milk. Milk samples were collected from thirty lactating buffaloes, divided into a control group and an oxytocin-treated group, and further subdivided by lactation. The samples were analyzed for pH, specific gravity, ash, protein, total solids, fats, lactose, and moisture content. The results showed that oxytocin treatment significantly decreased pH levels in milk from the first lactation, whi...
Abdul Kabir1*, Anees Ur Rahman2, Inzamam Ali Shah2, Ibad ur Rahman2
...al zoonosis that affects buffaloes, cows, and humans. It is caused by buffalopox virus (BPXV), a member of the genus Orthopoxvirus and a close variant of vaccinia virus (VACV). Buffalopox was first reported in India in 1934 and has since become an emerging and re-emerging disease in the Indian subcontinent and other countries. The disease is characterized by pock lesions on the skin and mu...
Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3
...e response of cattle and buffaloes against P. multocida B:2 in developing countries like Pakistan....
Hassanein, Suzan A.; Abd El-Wahab, Wafaa; Eweis, Moustafa and Mahmoud, Mervat M.
Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.
...ing 2009 from cattle and buffaloes. The higher percentage of positive was detected in Sharkia (31.7%) while Kafr Elsheikh was (27.3%). The positive result of detection of antibodies against non structured proteins of FMDV indicates that these samples come from natural infected animals. The further work is required to evaluate the performance of this method in different animal species and different field situations. The Prio check ELISA was also used for compar...
Zaghloull , A.H.; Mahmoud2, Amal; Hassanl , H.Y.; Hemeida2, A.A.; Nayell , M. A. and Zaghawal , A.A.
...tion of cattle and water buffalo. A sever outbreak was recorded in Egypt during 2000 and 2001. Using RT PCR, a primer designed to amplify 438 nucleotide fragments from the glycoprotein G gene allowed specific amplification of BEFV-cell culture isolates from Egypt (Menoufia and Alexandria Governorates) and Japan. The PCR product was sequenced and analyzed. PCR product of Alexandria isolate was cloned and labeled with digoxigenin and used as diagnostic probe for...
Abeer, E.Mansour and Hegazi, A.Z.
...ed in vaccinated cattle, buffaloes, sheep, goats and camels. Five animals of each species were vaccinated using a dose of I ml for sheep and goats and 2 ml for cattle, buffaloes and camels inoculated subcutaneously. It was found that both of the immune parameters were generally increased gradually to reach the highest value of cellular immunity by the 2e day, while that of neutralizing antibodies was reached by the wee...
Nahed A. Mohamed l, H. A. Hussein2, Fathia M. Mohamed1 and M. A. Shalaby2
...s from apparently health buffaloes. One hundred and thirty-two samples including buffy coat and organs were collected from apparently health Screening buffaloes in Cairo, Mansoura and Suez governorates in Egypt during the year 1999. Screening of samples by GAPT using standard known anti-BVDV antisera revealed that three positive samples for the presence of BVDV antigen. In a trial for the isolation of the BVDV, 69 buffy coat...
H. A. Hussein1, N.A. Mohamed2, F.M. Mohamed2 and M.A. Shalaby1
...d from apparently health buffaloes in cell culture was studied. The isolated viruses were classified into 3 groups based on the onset and severity of cytopathic effect (CPE). The cytopathogenicity of the isolates were different when these viruses were inoculated on BVDV-free and-infected cell lines. To study the discrepancies on the growth behavior of the local isolates, the viruses subjected to further passages on MDBK cells infected with non-cytopathic (NCP)...
S. A. Khalil1; M. El-Sayed2; M. M. El-Fayomy2; H. Y. Hassan3 and A. Zaghawa3
...n among cattle and water buffalo. The disease is characterized by inflammation of mesodermal tissue as achieved by muscular shivering, stiffness, lameness, and enlargement of the peripheral lymph nodes. Recently, the disease caused several outbreaks during 2000, 2001 and lastly 2004. The persistence of immunity after natural infection is varied from 6 months to several years. Assessment or maternal antibodies in calves born to naturally infected dams is a matt...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...ial insemination (AI) in buffalo was the quality of spermatozoa post-thawed. Therefore, research was conducted on the use of lycopene as an effort to improve the quality of spermatozoa post-thawing. This study aimed to assess the impact of incorporating lycopene into both the skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo
Elly Roza*, Salam N. Aritonang, Yulia Yellita, Hilda Susanty, Rizqan
...h economic value. Murrah buffalo is one of the milk-producing livestock with a total production that the average milk production for 294 days of lactation is 1.76 kg per lactation. Buffalo milk has an advantage 6–8% fat and 4–8% protein compared to 3–4% fat and protein content in cow’s milk. Generally, the maintenance of dairy buffalo is still traditional, so the pr...
Mahmoud Hamouda1*, Ahmed Al-Jazzar1, Fahad Al-Hizab1, Ahmed Azab2, Mohamed Al-Hammadi3
...gnificant role in female buffalo infertility. A total of 340 reproductive tracts of female buffaloes have a previous history of infertility was collected from different farms in Egypt through the period of two years. Uteri have been visually and manually inspected as well as examined bacteriologically and histologically. Several uterine lesions either external or internal were noted in 78/340 animals (22.94%) as follows: int...
Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2
...um) of two riverine type buffaloes using PCR-denaturing gradient gel electrophoresis (DGGE) method to profile the bacterial community and PCR amplification using species-specific primer sets for 16S rDNA fragments to detect major fibrolytic and non-fibrolytic bacteria. Results from the PCR-DGGE analysis showed differences in bacterial diversity, density, and banding patterns along the gut of buffaloes. Higher bacterial diver...
Ruth Dameria Haloho1*, Jisril Palayukan1, Agus Setiadi2, Edy Rianto2, Nadlirotun Luthfi3
...ors on the profit of the buffalo farming business used in funeral ceremony in West Sulawesi Province. This study used a survey method. The locations were selected by purposive random sampling for the highest population of buffalo. One hundred buffalo farmers were taken as respondents by a multistage random sampling method. The data were obtained by observation and direct interviews the far...
Ediset1*, Jaswandi2, Fuad Madarisa1, Amrizal Anas1, Rizqan2
...y of animal protein from buffalo can only be maintained by relying on an innovation-based maintenance system. This study aims to determine the level of adoption of Artificial Insemination (A.I.) innovations and the success rate of A.I. innovations in the buffalo farming business. This study uses a survey method and a secondary data analysis approach. The population is 100 buffalo breeders ...
KAFEEL AHMAD1, NIMRA ARSHAD1, ZAFAR IQBAL KHAN1, KINZA WAJID1, HUMAYUN BASHIR1, IJAZ RASOOL NOORKA2, MAHPARA SHEHZADI3, HAFSA MEMONA4, MADIHA SANA4, KHALID NAWAZ5, MUDASRA MUNIR1, IFRA SALEEM MALIK1, TASAWAR ABBAS6, ABRAR HUSSAIN7, MUHAMMAD SHER8, MUHAMMAD FAHAD ULLAH6, MUHAMMAD AKRAM QAZI9, MUHAMMAD NADEEM10, MUHAMMAD RIZWAN QURESHI10
...tion of forage, soil and buffalo blood samples to analyze heavy metals in them. Forages, soil and buffalo blood were collected from six sites of sampling. Out of which 5 sites were on the roadside and one site was away from the road. Grinding of the collected samples of soil and forage was followed by their acid digestion. Buffalo blood samples were centrifuged and the blood serum was sepa...
Wesam Jasim Hansh
...Fascioliosis in cows and buffaloes in Thi-Qar province, Iraq based on morphological measurements and sequences analysis of 28S rRNA gene. Fasciola flukes were isolated from the livers of cows and buffaloes slaughtered in the municipality of AL-Nassiriyah abattoir during July to November 2022. Sixty Fasciola flukes including (30 from cows and 30 from buffaloes) were used in morphological st...
MUSHTAQ HUSSAIN LASHARI1*, UMER FAROOQ2, AMMARA ARSHAD1, MUHAMMAD NAEEM TAHIR2, MUSADIQ IDRIS2, ANAM KHURSHID3 & ZIA UR REHMAN2
...ermination in cattle and buffalo. Blood samples of adult cattle (n=26) and buffaloes (n=26) grouped as males and females (n=13 each), were collected from the jugular vein through appropriate measures. Thin blood smears were stained using Field stain „A‟ and „B‟. The PMNs were counted and observed for Barr Bodies attached to them. Only the neutrophils with terminable lobes were examined and counted. The mean v...
Gokarna Gautam1*, Sajan Rokaya1, Bhuminand Devkota1, Saroj Sapkota2
...>Reproductive pattern in buffaloes (Bubalus bubalis) is affected by photoperiodicity, heat stress and availability of feed supply. The objectives of this study were to understand the seasonality pattern of breeding and calving in buffaloes in subtropical regions of southern Nepal and to characterize the ovarian cyclicity status during the months of long day length. Data regarding seasonality pattern of breeding and calving o...
Anisa Mushtaq1, Murtaz ul Hasan1*, Asim Shamim2, Muhammad Ali Abdullah Shah1, Muhmamad Arif Zafar3, Abdul Asim Farooq4, Aayesha Riaz1, Muhammad Kamran1 and Saif ur Rehman1
... from 384 cattle and 384 buffaloes screened revealed three tick genera and six tick species: Rhipicephalus microplus, Rhipicephalus decoloratus, Rhipicephalus annulatus, Hyalomma anatolicum anatolicum, Hyalomma anatolicum excavatum and Haemaphysalis punctata. Of those four species were confirmed on morphological basis, two ticks species Rhipicephalus microplus and Hyalomma anatolicum anatolicum whose morphological feature were overlapping with other identified...
Gokarna Gautam1*, Hari Adhikari1, Bhuminand Devkota1, Subir Singh2
...treatment of anestrus in buffaloes during low breeding season (April to June). Buffaloes in Group T1 (Ovsynch, n=25) received GnRH on a random day (d0), PGF2α on d7, GnRH on d9, followed by fixed timed artificial insemination (FTAI) at 16-20 hours after second GnRH. Buffaloes in Group T2 (CIDR-synch, n=30) were treated as in group T1 except that a CIDR containing 1.9 gm progest...
Fika Yuliza Purba1,4*, Andi Magfira Satya Apada1, Andi Ariyandy2, Irwan Ismail1,4, Subaedy Yusuf3,4
...ng cattle, sheep, goats, buffalo, pigs, etc. Therefore, this study aimed to examine hematological and biochemical profile of cattle, based on different stages of FMD infection. A total of 24 blood samples were collected from Bali cattle, then grouped into 4, based on the estimated age of lesion inflicted on the animals: Group A (no lesions), Group B (early stage), Group C (advanced stage), and Group D (recovery stage). The results showed that the white blood c...
Muhammad Asif Iftikhar1, Talat Naseer Pasha2, Saima Inayat1, Khalid Javed3 and Rahman Ullah4*
...s, Pattoki and Nili Ravi buffalo milk procured from Nili Ravi buffalo Research Institute (BRI), Pattoki. All other ingredients purchased from Sigma Aldrich Germany through local suppliers. Milk was standardized to 15% total solids with 3.5% fat. Skim Milk Powder (SMP) was used for the standardization of the yogurt. Milk was pasteurized at 82oC for 5 minutes and cool down to 43oC and at this temperature starter culture was ad...
Hasina Baloch1, Rahmatullah Rind2, Dildar Hussain Kalhoro1*, Shahid Hussain Abro1, Hubdar Ali Kolachi3, Saqib Kakar1, Muhammad Ibrahim3, Fayaz Ahned1, Hasnain Ali1 and Muhammad Mahesar1
...collected from lactating buffaloes with signs of clinical and subclinical mastitis respectively. The California Mastitis Test was performed to confirm mastitis. The sub-clinical cases were categorized by severity of infection with moderate severe, severe, more severe and most severe. All the 646 positive samples analysed, 41.95% were found with flacks, 7.73% with watery consistency, 15.01% with pus, 10.06% with blood tinge, 21.67% had odour while 3.56% were no...
Fitrimawati*, James Hellyward
...f cattle, goats and also buffalo, laying hens, broilers, local chickens and ducks. The data obtained were analyzed using the Analytical Hierarchy Process (AHP) method. This research produces the leading commodities competitively and becomes the priority to be developed in West Sumatera, such as the beef cattle, goats and also buffalos, broilers, local chickens and ducks. Based on competitiveness concept, the first important ...
Ashraf Khan1, Muhammad Waseem Khan2,3, Imrana Niaz Sultan2, Abdul Manan Kakar4, Saad Ullah5 and Afrasiab Khan Tareen2*
...te metals in raw milk of buffalos and cows from different farms of district Quetta. Fifty-six whole raw milk samples from cows and buffalos were collected and analyzed using atomic absorption spectroscopy for metal contents. Levels of metals (mean±SD) such as mercury (1.97±0.49 ppm), antimony (0.37±0.08 ppm) and aluminum (0.49±0.33 ppm) exceeded the maximum permissible concentration whereas, all o...
Abdul Aziz Khan and M. Jamil Khan
...heavy leather, depickled buffalo hide pieces were tanned. Comparative studies of shrinkage temperature, tensile strength and elongation of resultant leather indicate that chir pine solids produce
somewhat better leather as compared to that obtained by tanning with mimosa solids. It is concluded that chir pine bark solids can be successfully utilized for commercial production of light as well as heavy leather of desired qualities....
Anup Sarker1, Dibyendu Biswas1, Md. Fakruzzaman2, Gautam Kumar Deb3, S.M. Jahangir Hossain4, Md. Ashadul Alam2, M.A.M. Yahia Khandoker5, Ashit Kumar Paul1*
...te of pregnancy of water buffalo by intra-vaginal bio-stimulation after AI. The study was conducted from August 2022 to July 2023 in the selected coastal area of Bangladesh. Sums of 75 adult female buffaloes were selected. Age, breed, parity, and others were recorded during selection. The animals were grouped into three experimental groups (group A: natural service, group B: conduct AI only, group C: using penis-like device-...
Asad Alam1, Asad Ullah2*, Imad Khan2, Yaseen1, Rafiq Ullah1, Tahira Tayyeb1, Muhammad Hanif1, Maiz ur Rahman1, Muhammad Owais Khan1, Fatima Syed3, Raheela Taj3, Shumaila Gul4, Muhammad Sadeeq5 and Muneeb Islam6
...% crude protein (CP) and buffalo meat. Water temperature was maintained at 31oC using heater (500Watts) with thermostat. The results were recorded with significant value in the growth of fish during 4th to 9th weeks. The values of fish weight were recorded higher (P<0.05) in group 3 than the group 1 and group 2. The floc concentration, feed and physico-chemical parameters have positive effects on biofloc system at winter session for quality production of Ni...
Nasir Ali Tauqir1*, Asim Faraz2, Muhammad Imran Babar3 and Irfan Shahzad Sheikh3
... oat fodder in Nili Ravi buffalo bulls. Four cannulated buffalo bulls were used for in situ screening of oat fodder harvested at 100 days of age sown using different levels of phosphorous and phosphorous plus nitrogen fertilizers. Dry matter (DM) and crude protein (CP) content were higher (p<0.05) in oat fodder supplemented with P and NP fertilizer as compared to control. The supplementation of P fertilizer resulted in de...
Salam N. Aritonang1*, Yulia Yellita1, Dwita Saningtias2, Eka Putri2, Rizqan1
...c compounds that support buffalo health. It is predicted that turmeric flour supplements will enhance erythrocytes, hematocrit, hemoglobin, and leukocytes, as observed in the health of female Murrah buffaloes. Parameters measured in this study included hematology (erythrocytes, hemoglobin, hematocrit, and leukocytes) in Murrah buffaloes. The study involved four lactating Murrah
Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*
... performance of Egyptian buffalo might be determined by genetic variants, but it is also affected by environmental factors. The possible action of L-carnitine (LC) on the maturation of Egyptian buffalo oocytes has not been determined yet. This study aims to estimate the effects of supplementing LC on the in-vitro fertilization (IVF) of Egyptian buffaloes. We evaluated blastocyst and apopto...
Saba Mehnaz1*, Farhan Ahmad Atif2, Rao Zahid Abbas1, Muhammad Kasib Khan1 and Muhammad Saqib3
... Theileria annulata from buffaloes of Sheikhupura, Jhang, and Rawalpindi districts by using duplex PCR. A total of 1,152 blood samples of buffaloes were screened for epidemiological investigations. DNA extracted from blood samples was used for the amplification of the msp1β gene and cytochrome b gene by using single and duplex PCR. The prevalence of these pathogens and associated risk factors were observed through the m...
Elly Roza, Yulia Yellita, Hilda Susanty, Rizqan
...on and quality of Murrah buffalo milk in West Sumatra are still relatively low, and this is due to the feed provided not meeting the production needs of livestock. Hence, milk production and quality still need to be expected. This research aims to get the best forage feed by utilizing local forages (cassava leaves, sweet potato leaves, and Moringa leaves), which are high in nutritional content, to increase the production and quality of Murrah
Barkat Ali Jatoi1*, Amjad Hussain Mirani1, Abdul Latif Bhutto1, Ambreen Laghari2, Abdul Samad Magsi3, Ahmed Sultan Jatoi4, Aneela Sultan Jatoi5, Muhammad Mohsen Rahimoon6, Aarab Khan Lund7, Om Parkash1, Atif Ali Malak8
...hagic septicemia (HS) in buffalo at various talukas of district Shaheed Benazirabad (SBA) and to observe the efficacy of drumstick tree (Moringa oleifera) and river red gum (Eucalyptus camaldulensis) plant extracts against Pasteurella multocida. A total of 10 farms of each taluka of Shaheed Benazirabad district (i.e. Nawabshah, Qazi Ahmed, Daur and Sakrand) were visited to examine animals suspected of being infected with HS. A total of 2244
Danyal Khan1, Abdur Rahman1, Muhammad Shuaib2,3*, Sohaib Ul Hassan4, Abubakar Sufyan5, Wajid Ullah1, Seema Jani1, Obaid Ullah6 and Shahrood Ahmed Siddiqui7
...erformance of pre-weaned buffalo calves fed on different levels of milk replacers and their effects on feed intake, weight gain, body condition score, disease incidence, and overall profitability. Twelve calves were randomly selected and divided into four groups. Group A (control group) calves were fed with raw milk, while calves in groups B, C, and D were fed with milk replacer (MR) having levels of 13%, 16%, and 19% respectively. A Complete Randomized Design...
Mustafa Abd El Raouf*, Mostafa Abdelazim, Abdel Basit M. Abd El-Aal, Shebl E. Salem
...der affecting young male buffalo calves. The aim of the present study was to evaluate the efficacy of Walpole’s solution in dissolution of urethral calculi in buffalo calves with ruptured bladder in comparison to ammonium chloride. Therefore, twenty male buffalo calves aged 4-7 months admitted to the Clinic of Department of Surgery, Faculty of Veterinary Medicine, Zagazig University ...
Qamar Ullah1,2, Muti-ur-Rehman Khan1*, Raheela Akhtar1 and Aftab Ahmad Anjum3
...nducted on riverine-type buffaloes suffering from sub-clinical mastitis (SCM). The domestic water buffalo (Bubalus bubalis) provides milk, meat, hides, and draught power and gives a significant share to the agricultural economy of the globe. The river buffalo and the swamp buffalo are two distinct types of water buffalo
Thaer W. Enad*, Khalefa A. Mansour
...MD serotype SAT2 in Iraq buffalo. The study involved 102 buffaloes from two provinces, Wassit and Dhi-Qar. Clinical examination within the herd revealed classic FMD signs, including salivation, oral and nasal mucosal erosions/ulcerations lesions and laminas of affected animals. The proportions of older animals were more likely to show infection (p > 0.05), but there was no significant difference. The female was the predom...
Van Dai Nguyen1, ¥, Van Ba Nguyen2, ¥, Thi Thanh Nhan Giang2, Cong Dinh Nguyen2, Thi Lan Nguyen1, Dinh Ngoan Vu1, Duc Chuyen Nguyen1, Van Can Ta1, Khac Khanh Nguyen2, Thi Lien Cao2, Doan Lan Pham2, Khanh Van Nguyen2, Ngoc Tan Nguyen3*
... of two Vietnamese swamp buffalo populations, LangBiang (LB) in Lam Dong and Thanh Chuong (TC) in Nghe An Provinces of Vietnam. Ear tissue samples were collected from 96 buffaloes including 64 LB (32 LB-LD and 32 LB-LC) and 32 TC and a set of 18 microsatellite markers was used to analyze their genetic diversity. Results showed that all the microsatellite markers were polymorphic in the two local swamp <...
Nuttanun Leamkrajang, Somkiert Prasanpanich, K. Teepalak Rangubhet, Phongthorn Kongmun*
...ee animal species (swamp buffalo, beef cattle, and goat), while the dietary treatments consisted of two roughages to concentrate ratios: 100:0 and 70:30, using dry leucaena leaves as the source of roughage. The kinetic values of fermentation, gas production, and IVDMD showed significant differences across treatments (p<0.05). The swamp buffalo and the 70:30 ratio exhibited the greatest gas production and IVDMD, while beef...
Ray Adil Quddus1, Nisar Ahmad1*, Jalees Ahmad Bhatti1, Anjum Khaleeq2 and Asfand Yar Khan3
...us events episodes of 60 buffaloes having 2nd to 5th parity were studied either automated monitoring system or visual observation. The rate of true estruses detection was higher when estruses were detected by an automated monitoring system than visual observation (30 vs 17, respectively; P<0.05). However, false estruses prediction was lowered by an automated monitoring system than visual observation (11 vs 19, respectively; P<0.05). Therefore, efficiency...
Hind Abdulzahra Abdulkadhim Alshaibani1*, Abdul Jaleel Aziz Karim Alqaraghli2, Mohammed Azeez Yasir Alzaidi3
...htered animals (cows and buffaloes) in Iraq. In the central slaughterhouse of Nasiriyah, 58 F. gigantica adults were collected from the livers of cows and buffaloes. We observed that buffaloes were infected with fasciolosis at a rate of 15.29%, higher than the incidence of fasciolosis in cows (10.66%). We recorded a greater prevalence of fasciolosis in females than in males, as the prevale...
Batool S. Hamza1*, T.S. Al-Amery2
...productive systems of 50 buffalo cows slaughtered at the Basrah slaughterhouse. Fifty genitalia of female cows were confiscated from a nearby slaughterhouse. The results showed gross inflammatory changes of endometritis in 50 animals and classified according to histological examination as acute endometritis (24 %; 12/50), subacute endometritis (18 %; 9/50) chronic endometritis (30 %; 15/50), and while metritis cases (16 %; 8/50) were of the chronic type of inf...
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