Muhammad Imran1*, Muhammad Mobashar2, Muhammad Irfan3, Shazia Hanif4, Sumaira Hanif5 and Muhammad Abubakar6

...) in lactating Nili-Ravi buffaloes. On the basis of nearly similar lactation stage and body weight, sixteen individually confined buffaloes were randomly blocked and allotted to four different dietary treatments. MOLM in proportion to CSC was supplemented along with maize silage as basal diet. Four dietary treatments having MOLM were 0.0, 0.71, 1.42 and 2.16 Kg DM/day used as replacement for CSC containing 2.16, 1.42, 0.71 a...

 Arif Khan, Abdur Rahman, Shoail Akhtar, Siraj Uddin, Zahid Ullah and Nazir Ahmad

 
...ustify;"> Pakistani buffaloes beef were analyzed for various quality characteristics in relation to breed and age. A total of one hundred and eight beef samples from Longissimus dorsi muscle were collected from three age groups (i.e. group 1= 12-18 monts, 2 =18.1-36.0 months and 3= > 36 months) of Kundi and Nili Ravi breeds of buffalo. Samples were obtained from freshly preselected slaughtered <...

Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*

... on post-thaw quality of buffalo bull semen. For this purpose, two consecutive ejaculates were collected from three Nili-Ravi buffalo bulls using artificial vagina at weekly intervals for a period of three weeks (three replicates). Qualifying semen ejaculates were diluted (50×106 motile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4&d...

KM Fakhrul-Islam, Mohammad Shah Jalal, Sonnet Poddr, Md Nurul Quader, Md Sahidur-Rahman, Avijit Dutta and Shuvo Mazumder

...isease affecting cattle, buffalo, sheep, goats, and wild animals around the globe. This highly contagious disease causes severe economic loss due to reduced productivity of the affected animals as well as increased mortality in calves and kids. In this study proportionate prevalence of FMD in cattle was estimated and the distribution of FMD according to different factors was evaluated. Both retrospective and prospective FMD clinical cases were included conside...

Neelum Andaleeb and Munir Khan

...Average milk produced by buffalo and cow was 180 and 135 liters per month, respectively. Average total costs of milk production of buffalo and cow were Rs.12835 and Rs.10070 per month respectively whereas the average net income per liter from the buffalo and cow milk was Rs. 29 and Rs. 5 thus benefit-cost ratios for buffalo and cow is1:1.43 and 1:1.07, r...
Hafiz Aftab Ahmed1, Asif Ali2, Akbar Ali2, Salman Ahmed Abid3 and Sajid Umar4*
...Three cases, including a buffalo and two cows were presented with complaint of persistent unilateral hind limb extension. The animals were unable to flex the limb during walk. Diagnosis was based on case history, clinical sign and clinical examination. Upward fixation of patella was diagnosed in all three animals. After careful palpation of the ligament, medial patellar desmotomy was performed in lateral recumbence, under local anesthesia achieved by 2% lignoc...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
... biochemical response in buffaloes to Gigantocotyle explanatum infection. Of the 558 slaughtered buffaloes from central Punjab, Pakistan, 18.99% were infected, and morphometric analyses were made of 115 mature amphistomes and gravid eggs with traditional microscopic methods on the basis of standardized measurements. Amphistomes were flattened and stained in Borax carmine, some were sectioned in the median sagittal pla...
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...bortus in cattle and buffaloes in the districts of Islamabad and Rawalpindi, Pakistan. A total of 120 serum samples were randomly collected from buffaloes and cattle (60 per species) at Civil Veterinary Hospitals, animal markets and peri-urban livestock holdings in Rawalpindi and Islamabad. Serum samples were initially screened by the Rose Bengal Plate Test (RBPT). RBPT positive samples were subjected to a B. abortus<...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...gestibility in Nili Ravi buffaloes. Four lactating Nili-Ravi buffaloes of 3rd and 4th parity and milk production of 8±2.3 kg/day were selected and offered a basal diet based on sorghum forage and concentrate ration supplemented with fibrolytic enzyme (Fibrozyme™, Alltech Inc. Company, USA) at four levels (T1 = 0, T2 = 10, T3 = 15 and T4 = 20 g/animal/day) in a 4 × 4 Latin Square chang...
 

Amir Ismail1, Muhammad Riaz1,2*, Saeed Akhtar1, Amjad Farooq3, Muhammad Arif Shahzad1 and Ahmad Mujtaba4

 

...the milk samples of cow, buffalo and goat. The level of Pb was found to exceed the maximum permissible limit (0.02 µg/g) set by Codex Alimentarius Commission in 53% milk samples. In 44% milk samples the level of Cd was found to exceed the permissible limit (0.0026 µg/g) set by International Dairy Federation (1979). The mean levels of Ni, Cu and Co were found in the normal ranges. The data for estimated daily intake of heavy metals through milk show...
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...AO staining protocol for buffalo sperm which has not been explored yet. Semen was collected from two mature buffalo bulls, diluted in tris citrate egg yolk based extender and cryopreserved using standard protocol. The semen straws were thawed at 37oC and stained using different protocols as followed: Protocol 1 (washing effect); washing either with normal saline (NS) or phosphate buffered saline (PBS), Protocol II...
Shahid Hussain Farooqi1,*, Muhammad Ijaz1, Muhammad Hassan Saleem1, Muhammad Imran Rashid2, Muhammad Oneeb2, Amjad Khan3, Amjad Islam Aqib1 and Shakeel Mahmood4
...nd one hundred and fifty buffaloes. Descriptive statistics with Pearson’s Chi-square test and regression model were applied to analyze the data. The results of study found Rhepicephalus the most prevalent genus followed by Heamaphysalis, Hyalomma, Dermacenter and Amblyomma with the prevalence of 78.50%, 10.33%, 10.08%, 0.67% and 0.42%, respectively. On species basis, R. (Boophilus) annulatus, R. (Boophilus) microplus, <...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2 
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...mic losses in cattle and buffalo. So rapid and accurate diagnosis of FMD is uttermost need of this time. Several lab.techniques are developed for rapid detection of FMD but these are expensive and time consuming. Elisa, cell culture, Reverse Transcriptase (RT-PCR) and real time PCR need specific high quality equipments in lab. Whereas Lamp PCR is simple and accurate test for the rapid diagnosis of FMD. This study has been done initially with FMD known serotype...
Kashif Prince1,*, M. Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Jawaria Ali Khan1, Nisar Ahmad3,Rais Ahmed2, Aamerzish Mushtaq4, Sajid Umar4 and Yung-Fu Chang5
...sk factors in cattle and buffaloes of district Kasur, Punjab. Selenium status was evaluated by Atomic Absorptions Spectrophotometery (AAS) with respect to sex of animals, geographical area, age of animals, herd size, stage of animals, production level and concentrate feeding. Selenium deficiency was also evaluated as a risk factor of mastitis and repeat breeding. About 48.43% cattle and 72.39% buffaloes were found positive i...

  Abdul Hassan*, Muhammad Ishaq*, Nisar Ali Shah* and Arshad Farooq*

MILK PRODUCTION POTENTIAL IN KHYBER PAKHTUNKHWA
...ith 1579 lit of milk per buffalo per year, 663 lit of milk per cow per year, 95 lit milk per goat per year and 64 lit of milk per sheep per year. The data in comparison to the National and World average, is far below from probable production levels. Except in buffaloes and goats where the farm level production of milk is higher than the World and sheep where the farm level production of milk of Khyber Pakhtunkhwa is higher t...

 Arshed Bashir*, Fatima Ahmad**, Irfan Mehmood*, Muhammad Qasim*, Mazher Abbas* and Sonila Hassan*

ECONOMICS OF RED MEAT PRODUCTION IN PUNJAB
... farms were dominated by buffaloes. The mean age, sale price and live weight of sold buffaloes was 2.8 years, Rs. 64963.8 and 157 kg, respectively whereas for cows the mean age was 3 years with average sale price of Rs. 65722.1 per cow and live weight of 210 kg. The cost of production of meat animals (buffaloes and cows) was higher in commercial farms (Rs. 67820.2 and Rs. 62773.5, respecti...

 Khalid Mahmood Aujla* and Abid Hussain*

ECONOMICS OF MILK PRODUCTION OF MAJOR DAIRY BUFFALO BREEDS BY AGRO-ECOLOGICAL ZONES IN PAKISTAN
...eceived from major dairy buffalo breeds (Nili-Ravi and Kundhi) in various agro-ecological zones of Pakistan. For this purpose, 219 buffalo farmers were randomly selected from mixed and rice-wheat cropping zones of Punjab and Sindh provinces, mixed cropping zone of Khyber Pakhtunkhwa (KPK) province, coastal zone of Sindh and mountainousAJK. Of these, 155 and 64 were Nili-Ravi and Kundhi buffalo...

 Ghazala Nawaz*, Muhammad Nawaz Malik**, Muhammad Hassan
Mushtaq***, Fraz Munir Ahmad****, Ali Abdullah Shah*****, Farooq
Iqbal******, Shinawar Waseem Ali*******, Zahida Fatima******** and
Amjad Khan*********

SURVEILLANCE OF BRUCELLOSIS IN LIVESTOCK IN RURAL COMMUNITIES OF PUNJAB, PAKISTAN
...ence per 1000 animals in buffaloes was (7.86%), significantly (P<0.01) higher than small ruminants (5.84%) and cattle (2.05%). Being a subtropical region, geographically significant (P<0.01) variation was observed in burden and pattern of brucellosis i.e., the highest prevalence was in Central (14.81%) followed by South (0.5%) and northern (0.4%) Punjab. Considering the high mean prevalence and its zoonotic importance it can be devastating if not control...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
...y weight etc. in cattle, buffalo, goat and sheep worldwide. LEP gene polymorphism and associations have not been extensively reported in Lohi sheep of Pakistan, so this study aimed to find any variations in gene sequence and possible association with growth rate to explore meat potential of this breed. A total of 18 Lohi animals were selected from the flock. After the DNA extraction from blood samples, a 1486 bp fragment of exon three was amplified and sequenc...
Abid Hussain1,*, Mansur-ud-Din Ahmad2, Muhammad Hussan Mushtaq2, Mamoona Chaudhry2, Muhammad Sarwar Khan2, Michael Reichel3, Tanveer Hussain4, Amjad Khan2, MuhammadNisar2 and Imtiaz Ahmad Khan5
...inical mastitis in dairy buffaloes. The present study was conducted in two districts Lahore and Bhimber Azad Kashmir Pakistan. Total sample size for this survey was consisted of 1,036 lactating buffaloes. The screening of lactating buffaloes was performed with the California Mastitis Test. The overall prevalence of mastitis was recorded at 49%. Among mastitis cases the prevalence of clinic...
Abdul Majid Ujjal2, Sharmin Akter1* and Suman Paul1 
...e hospital prevalence of buffalo diseases and to determine the temporal distribution of important infectious diseases of buffalo like as Foot and Mouth Disease (FMD) and Haemorrhagic Septicaemia (HS) at Upazila veterinary hospitals of Jaintapur, Gowainghat and Kanaighat upazilas of Sylhet district. From October, 2015 to September, 2017 a total of 1057 cases of buffalo diseases were recorde...

Gulsanga1, Zahin Anjum1, Sahib Alam2* and Farhat Shehzad

...d P) contents of cow and buffalo muscle meat obtained from University Campus, Peshawar. The data showed that Cu, Zn, Pb and Ni concentrations in cow meat ranged from 7.27 to 14.81, 64.00 to 112.12, 3.30 to 6.25 and 0.73 to 2.53 mg/kg whereas the concentrations of these heavy metals in buffalo meat varied from 3.15 to 7.71, 72.88 to 88.47, 1.41 to 13.28 and 0.38 to 1.93 mg/kg, respectively. The average Na, K, Ca, Mg and P con...
Hasina Baloch1, Rahmatullah Rind2,*, Muhammad Rafique Rind3, Viram Kumar4, Nazia Baloch5 and Rajesh K. Oad6
... subclinical mastitis in buffaloes. Significantly higher prevalence (49. 07%) of clinical mastitis was observed in buffaloes with average age of 6 to 9 years whereas those of > 9 years of age showed higher (33.33%) but non-significant prevalence of subclinical mastitis. However, significant impact of the number of parity on the prevalence of clinical mastitis was observed in animals. Higher prevalence (48.20%) o...
Selçuk Kaplan
...tions in Anatolian water buffaloes. Sequence analysis were revealed seven polymorphic site (G1072A, T1081C, T1131G, T1143C, T1145G, T1151G and C1221T) and one monomorphic site (C1071T) in Anatolian water buffaloes. To the best of my knowledge, this is the first study that fully describes seven polymorphic sites of bubaline leptin gene in Anatolian water buffaloes.
...
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...le (Bos indicus), buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Capra hircus). Selected marker V158M is present in exon-8 of COMT gene. Each specie was subjected to blood sampling (n=20 each). DNA was extracted, purified, quantified and amplified through PCR by using specific primer set. Then Sanger’s method of DNA sequencing was used. Multiple sequence alignment demonstrated that sheep and goat were having the wild ...
Nuzhat Huma1, Fozia Ghaffar1, Saima Rafiq2,*, Imran Pasha1, Aysha Sameen1, Imran Hayat2 and Imtiaz Hussain2
...erent dairy animals like buffalo, cow, sheep, goat and camel. The buffalo and sheep milks have comparatively higher fat, solid-not-fat and total solid contents than other milks. Maximum whey proteins were found in the sheep milk (0.78%) whereas cow milk had lowest contents (0.54%). Non-casein-nitrogen (NCN) contents in sheep milk were higher followed by camel and buffalo milks. Electrophor...
Azmat Hayat Khan Khattak1, Muhammad Nauman ul Islam1, Muhammad Shakir Khan1, Muhammad Iqbal1, Imtiaz Ali Shah1,Sheeraz Ghani1, Amjad Ali2, Zubina Hameed1, Mohammad Farooque Hassan3 and Tariq Ali1,3,*
...AJC) and eleven Azakheli buffalo calves (ABC) were reared on milk replacer to compare their growth performance and economics. The experiment was performed at Livestock Research and Development Station (LR&DS), Surezai, Peshawar for a period of 12 weeks. The calves were fed with milk replacer (10% of their live body weight) in two equal doses, one in the morning followed by an evening dose, until the end of 8th week. The average birth weight of A...

Shaista Naz1*, Noor Paio Khan1, Naveed Afsar2 and Ashfaq Ahmad Shah3

...t women were involved in buffalo, cattle, goats and sheep rearing with higher possession of small ruminants (goats and sheep), while men were found dominant in large ruminants’ possession (buffalo and cattle). Women’s higher time allocation to livestock management activities depicted their higher level of participation in livestock management was found high as compared to their male counterparts. Women’s pa...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2

 

... total of 600 cattle and buffalo were selected from three different topographical locations of Punjab on the basis of clinical biomarkers. Blood and mosquito samples were collected. Blood samples were processed through complement fixation test and RT-PCR, while mosquito samples were processed through RT-PCR. Overall prevalence of bovine ephemeral fever (BEF) in indigenous breeds of bovine was 63.33%, lower, than exotic breeds 64.66% which were found to be more...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro
...thawed quality of Kundhi buffalo bull semen. After collection ejaculate was brought in Semen Production Unit. Semen was evaluated as fresh for volume, colour, motility, morphology, live dead ratio, membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integrity. Semen volume and colour were determined by visual examination,...

M. Tariq Javed1, Nazir Ahmad2, Gulbeen Saleem3, Haleema Sadia4, Farkhanda Manzoor5 and Ghulam Hussain Dilbar

EFFECT OF AGE AND SEASON ON SEMEN CHARACTERISTICS OF BUFFALO BULL - A REVIEW
...s of semen parameters in buffalo bull at different age and in different seasons has been published with conflicting reports. An effort is made to review the literature gathered from different sources on semen characteristics in buffalo bulls which will be helpful to researchers and allied professionals in future research or policy planning. The review is bifurcated in different sections for easy understanding of the facts. T...
Muhammad Kashif Maan1, Ghulam Mustafa1, Munibullah2 and Sajid Umar2,*
...014 to 2015, twenty five buffalos ranging in age between 7 to 9 years, were referred to surgery clinic at University of Veterinary and Animal Sciences, Lahore, Pakistan. The patients showed the signs of ocular lesions associated with plaque formation on the cornea and sclera and papilloma formation on the lateral limbus of eye. The tumors were large and usually protruded through the palpebral fissure and infiltrate the entire eye ball. Diagnosis was made based...

 Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2

...ous cattle, sheep, deer, buffalo and goat, etc. Various synthetic anthelmintics have been used, on wide scale, to control the helminths infections.But the parasite worms have developed multiple resistances against the synthetic anthelmintics. Hence, various biological controls, vaccines and local medicinal plants are being explored to control the parasitic worms. The aim of this study was to evaluate the anthelmintic potential of two local plants, Albi...

Rafiullah1*, Abdur Rahman2, Khalid Khan1, Anwar Ali1, Arifullah Khan2, Abdul Sajid3 and Naimatullah Khan3 

...imal species (cattle and buffalo) were selected. A total of 800 samples were collected from different areas of District Lakki Marwat and District Peshawar. All the samples were processed through different diagnostic techniques, including Microscopy, ELISA and Real-time PCR for diagnosis of Theileria parva at Veterinary Research Institute Peshawar. The overall prevalence for Theileriosis was 27.75% through Real-time PCR, followed by Indirect ELISA (24.62%) and ...
Abdul Wahaab1, Muhammad Ijaz1,*, Syed Saleem Ahmad1, Umair Iqbal2, Abdul Tawaab1 and Iahtasham Khan3
...ated bovines (cattle and buffaloes) and their effect on various blood parameters (WBC, RBC, Hb, HCT and MCHC). For this purpose, 40 infected animals were selected. After dividing them randomly in four groups of 10 animals each, drug was administered to first three groups whereas last one was kept under positive control for comparison. Study revealed that Triclabendazole was most effective drug of all three in both cattle and buffalo

 Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1

... collected from cows and buffaloes kept at different localities in district Kasur, Punjab, Pakistan. The possible risk factors inquired from farmers were animal type, breeds, urbanicity, age (years), teat washing, bedding area, lactating stage and previous exposure of mastitis. Initially surf field mastitis test (SFMT) was applied for screening subclinical mastitis followed by bacteriological techniques on positive milk samples for confirmatory isolation of
Fazal Abbas1, Muhammad Ijaz1,*, Zunaira Akhtar1, Khalid Mehmood2, Muhammad Zeeshan Hyder3 and Umair Iqbal4
...n=60 cattle calves; n=60 buffalo calves) under one month of age irrespective of cause of diarrhea was selected from different livestock farms located in the outskirts of Lahore. The result showed that there was increased in packed cell volume (%) in their blood profile. The sodium and potassium concentration in ppm significantly increased (P< 0.001) while the calcium, copper and iron concentration in ppm significantly decreased (P< 0.001) in the calves h...

Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3 

... mastitis in a cohort of buffaloes at Khyber Pakhtunkhwa. 120 quarter samples were collected from suspected buffaloes in selected areas of Peshawar, Charsadda, Mohmand Agency, and Dara Adam Khel. Initially, California Mastitis Test was performed for screening of positive samples. Afterward, the bacterial profile was confirmed through biochemical testing. The quarter wise prevalence of subclinical mastitis was 25%. Within thi...
Asghar Khan1,*, Aneela Zameer Durrani1, Arfan Yousaf2, Jawaria Ali Khan1, Mamoona Chaudhry1, Mumtaz Ali Khan3, Habibunnabi4 and Amjad Khan5
...al mastitis in lactating buffaloes in Pothohar region of Pakistan. A questionnaire based cross-sectional based survey was conducted in 30 commercial and subsistent farms in Pothohar region of Rawalpindi district, Punjab. Where randomly 196 lactating buffaloes were sampled. Data on several health, management and bio-security variables was collected in face to face interviews from farmers, managers and owners. Milk samples col...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
... and compare the role of buffalo oviductal epithelial cell (BOEC) co-culture and conditioned culture media, on in vitro development of Nili Ravi buffalo embryos. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertiliza...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
...dy, BMP15 gene of buffalo was cloned, analyzed, and its’ expression pattern was further determined. It consists of 1185 nucleotides that encodes 394 peptides bond. The BMP15 gene was observed to be expressed in cumulus tissue, granular tissue, hypophysis, genital ridge and tissues of ovary and testis. Moreover, QRT-PCR results showed that BMP15 was expressed in the whole process of embryogenesis and folliculogenesis, early high level...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...ic disease of cattle and buffaloes, causing progressive weight loss, persistent diarrhea and finally death. Due to zoonotic nature of the disease, workers in slaughterhouses are at a high risk of infection. In this study, the status of paratuberculosis was assessed in a slaughterhouse located in district Faisalabad, Pakistan. A total of 455 blood samples were collected from slaughtered cattle and buffaloes at random and then...

Muhammad Abdul Rahman1*, Abdul Saboor1, Gulnaz Hameed1, Ghulam Bilal2 and Farooq Tanwir3 

...seases, sensitiveness of buffaloes to temperature, slow and reduced weight gain and effect on birth and death rate are weighted high as threat to farm animal health while India-Pakistan ties on water, water scarcity, high water requirement for buffaloes and lack of innovation in processing of milk and milk products are other major threats to production from farm animals. The findings of this study can serve as basis of provi...
Doulat Khan1, Hamayun Khan1, Nazir Ahmad2, Muhammad Tarique Tunio3, Muhammad Tahir2, Muhammad Saleem Khan2 and Rifat Ullah Khan1*
...dentification in cattle, buffalo, goats and sheep. A total of 120 blood were taken from jugular vein of different breeds of cattle (Achai, Achai x Jersey, Holstein Friesian and Jersey), buffalo (Nilli Ravi, Aza Kheli and non-descript), goats (Beetal, Teddy and non-descript) and sheep (Bulkhi, Karri and non-descript). In cattle, the average sensitivity, specificity, false pregnancy prognostic value, false non-pregnancy progno...
Savas Atasever*, Huseyin Erdem and Ibrahim Cihangir Okuyucu
...men fill scores (RFS) in buffaloes. In total, 66 Anatolian buffaloes reared in four farms of the Middle Black Sea region of Turkey were scored subjectively by two parameters. BCS and RFS data were obtained using a 1 to 5 scale and a 1 to 4 scale, respectively, where lower points indicate thin body condition or relatively empty rumen. Animals were examined in two subgroups by parity (1= lactation number 1 and 2; and 2= l...
Veli Sel1, Isa Yilmaz2,* and Mete Yanar3
...farms of Anatolian water buffalos in Igdir. For this purpose, a total of 637 milk samples gathered from 91 animals raised in 54 farms were analyzed and statistically evaluated. The age and education level of the farmers and the effect of the milker on the SCC were found to be significant (P <0.001). SCC value (65.930 ± 2.484 cells/ml) was found to be the lowest when the milker is the person from the household. The udder cleaning before milking was a ...
Saeed Murtaza1, Abdul Sattar1*, Nasim Ahmad1, Muhammad Ijaz2Maqsood Akhtar3 and Muhammad Shahzad4
...oxytocin in newly calved buffalo has made buffalo breeder anxious about its reproductive and productive performances. Moreover, misconceptions about its harmful effect on public health are increasing day by day during postpartum period when animal has been already in stress. To know effect of oxytocin injection on cervix, uterus and milk composition during postpartum interval in Nili-Ravi buffalo
Ertuğrul Kul*
...) records from Anatolian buffaloes calved during 2014-2017 raised under different farm conditions in Amasya Province, Turkey. Five different lactation curve models such as Wood (WOD), Cobby and Le Du (CLD), Exponential (EXP), Parabolic Exponential (PEXP) and Quadratic (QUA) models were used. The adjusted multiple coefficient of determination (R2adj) and the residual standard deviation (RSD) were evaluated as parameters to detect th...
Hifzulrahman1, Muhammad Abdullah1*, Muhammad Uzair Akhtar2, Jalees Ahmed Bhatti3, Muhammad Saadullah1 and Muhammad Naveed-ul-Haque2
...arly-lactating Nili Ravi buffaloes. Sixteen early-lactating Nili Ravi buffaloes received 2 treatments in a completely randomized design with a period length of 120-d. The treatments were 1) Ctrl = basal diet without supplementation of fat, 2) Ca-FA = basal diet with calcium salts of palm fatty acids supplementation. The Ca-FA treatment increased the 3.5% fat-corrected milk yield by 11.0% compared with the Ctrl, whereas, the ...

Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan

...tating indigenous cattle/buffaloes raised on different commercial dairy farms and exposing the hazards linked with milk-born tuberculosis infection in the area where unpasteurized milk is normally used in the central zone of Khyber Pakhtunkhwa (KP), Pakistan. A total of 1225 cattle and 1175 buffaloes of more than one year were studied for the prevalence of bovine tuberculosis. A total of 1608 milk samples (793 from cattle an...

Tingting Li, Shuangshuang Geng, Long Xie, Huiyan Xu, Aolin Luo, Pengwei Zhao, Huan Yang, XingWei Liang, YangQing Lu, XiaoGan Yang* and KeHuan Lu*

...nswer this question, the buffalo GDNF gene was cloned and integrated into the PiggyBac eukaryotic expression vector, which was stably expressed in Sertoli cells, thus establishing a PB-GDNF-Sertoli cell line. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that the GDNF gene expression in the PB-GDNF-Sertoli cell line was significantly increased compared with that in the control Sertoli cell line (p<0.01). Enzyme-linked immunosorbe...

Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2

A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
...the households possessed buffaloes. Average number of buffaloes was estimated at 4.28 while 376 (63%) of the household reported cattle with average number of 4.38 heads per household. Small numbers of buffalo and cattle bulls were recorded. Average number of 5.55 goats per households was reported by 35% of all the households. On an overall basis, 2,365 liters of bu...
Shahid Ali*, Aijaz Ali Channa, Waqar Ahmad, Rafia Akram and Imbasat Yousaf
...rm pluriparous Nili Ravi buffalo (Bubalus bubalis) that was recumbent and under extreme stress because of intemperate bilateral abdominal distension. Trans-rectal palpation revealed a taut uterus (bulged, tense and inelastic) and alive fetus. Subsequently upon induced cervical dilatation, 60-70 L of amniotic liquid was expelled out and dam delivered completely developed alive male calf with a gross anomaly (presence of a hole in the skull) which could n...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
...reservation of Nili-Ravi buffalo bull semen. The semen samples were collected from Nili-Ravi buffalo (Bubalus bubalis) bull kept at SPU Qadirabad, District Sahiwal, Pakistan. Qualifying semen ejaculates having motility >60%, volume >5-6ml and concentration >0.5 billion/ ml were diluted 50 × 106 motile sperm ml approximately at 37°C in Tris-citric acid extender supplemented with different ...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...rtility of cryopreserved buffalo spermatozoa by addition of arachidic acid in semen extender. For this purpose, semen was collected from 3 adult buffalo bulls (Bubalus bubalis) of same age by artificial vagina keeping temperature at 42°C for a period of 5 weeks (replicates; n=30). Ejaculates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temper...
Muhammad Aamir Naseer1, Amjad Islam Aqib2*, Ambreen Ashar3Muhammad Ijaz Saleem1, Muhammad Shoaib4Muhammad Fakhar-e-Alam Kulyar1, Khurram Ashfaq1, Zeeshan Ahmad Bhutta1 and Shagufta Nighat5
...) from cattle (n=90) and buffalo (n=160) were aseptically collected from various dairy farms and put to biofilm detection and antibiogram. Based on collected data with statistical inferences, the study found 61.60% of S. aureus from subclinical samples, while 72.73% of S. aureus were positive for biofilm with uniform hike in samples from cattle (77.55% bpSA) and buffalo (70.48% bpSA). Udder condition/consistenc...
Mudussar Nawaz1, Iahtasham Khan2, Muhammad Shakeell*, Arfan Yousaf1, Zahid Naseer1, Munibullah1, Ali Zohaib3, Riaz Hussain4 and Qudrat Ullah5
...alence of brucellosis in buffaloes and goats of Islamabad Capital Territory, Pakistan. A total of 341 milk samples from buffaloes (n=180) and goats (n=161) were screened by (Milk Ring Test) MRT and milk i-ELISA for anti-Brucella antibodies. Higher prevalence was found in buffaloes (5.6% and 16.1%) than in goat (4.97% and 1.9%) both through MRT and i-ELISA respectively. Commercial pr...

Masoud Hassani1* and Omid Madadgar2,3

Serological Evidence of Bluetongue in Iran: A Meta-Analysis Study
...ne, cow, cattle, bovine, buffalo, camel, Iran and prevalence alone or in combination in both English and Farsi language. After reviewing 82 published articles, a total of 48 studies from 29 articles were eligible to be included in this meta-analysis study. The total seroprevalence of bluetongue in apparently healthy sheep, goat, cow and camel at animal level based on ELISA test was 50.4% (95% CI= 43.5–57.2), 79.2% (95% CI= 70.7–85.8), 3.3% (95% CI=...
Adnan Khan1, Rifat Ullah Khan1, Muhammad Subhan Qureshi2Muhammad Mobashar3, Ali Gohar4, Shakoor Ahmad1, Kamran Khan5Hamayun Khan1 and Shabana Naz6*

 

Said Sajjad Ali Shah1*, Muhammad Ilyas Khan1, Aziz Ullah1, Hayat Ullah2 and Faisal Ahmad2

Coprological Examination of Small and Large Ruminants in Central Zone of Khyber Pakhtunkhwa
... 51.5% in cows, 58.3% in buffalo, 73.9 % in sheep and 68.5% in goats. Trichostrongylus (32.8%) was recorded as highly prevalent in cattle followed by amoeba (10.2%) and Fasciola (6.1%), while in buffaloes fasciola was recorded higher followed by amoeba (19%) and Trichostongylus (5.35%). Haemonchus contortus was the most prevalent intestinal parasite in the study area followed by Trichostrongylus in small ruminants, whereas m...

Sarhad Journal of Agriculture

December

Vol. 36, Iss. 4, Pages 1010-1324

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