Shakeel Ahmed* and Sarwat Jahan

...Enzyme Immunoassay (EIA) kits. 48 h fasting significantly increased plasma adiponectin (P<0.001), while decreased leptin (P<0.001) and resistin (P<0.01) concentrations compared to normal fed monkeys. No significant (P>0.05) change in adiponectin levels was observed after NMS injection in both normal and metabolically stressed conditions. NMS administration induced a significant (P<0.01) increase in resistin levels, while suppressed leptin (P<...

Rongrong Li1, Kun Li2, Xiaoqiang Wang2, Houqiang Luo2, Gang Qiu2, Hui zhang2, Yanfang Lan2 and Jiakui Li2,3*

...nked immunosorbent assay kits and an indirect hemagglutination test. The results showed that seroprevalence in Tibetan pigs were 25.6% by ELISA. On gender basis, the prevalence was found 25.1% in males and 27.8% in females. By the method of IHA, the seroprevalence in Tibetan pigs were 21.6%, and the prevalence was found 23.1% and 21.4% in males and females, respectively. The results indicates that the Tibetan gigs were exposed to T. gondii in Tibet area, which...
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...ts also indicated that c-kit expression was higher in the white cashmere goat than in the black goat, and this significant difference was detected by q-PCR and western blotting. All cashmere goats of different colors examined by immunohistochemical analysis showed either weak (the black cashmere goat) or strong (the white cashmere goat) expression of the KIT protein. These findings ...
Tariq Mahmood*1, Syed Wasif Ahmed Shah1, Muhammad Rais1, Hira Fatima1, Faraz Akrim1 and Muhammad Sajid Nadeem2
...ting purpose while black kite Milvus migrans utilized semal tree Bombax ceiba. The weaver bird Ploceus philippinus also utilized only Acacia nilotica for nesting. The common crow, common myna, black kite, black drongo, red turtle dove Streptopelia tranquebarica and Indian robin Saxicoloides fulicatus successfully bred in particular nests whereas red wattled lapwing Vanellus vanell...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
...ence of EIV. Using ELISA kit and HI as serological methods for diagnosis, we found a 24.13% (168/696) overall seroprevalence, where the seroprevalence for H3N8 was 14.51% (101/696), for H7N7 6.03% (42/696), and 3.59% (25/696) as mixed infections, suggesting EIV current and active circulation in equine population of Pakistan. Statistical analysis suggested predicting variables including “local equine density per 2 KM”, “equines workings in pon...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...racted using R Neasy Minikit (Qiagen) according to instruction manual. After extraction RNAs were eluted in 60 ul elution buffer and stored at -70C. Each strain of FMD virus was serially diluted to -5dilution in order to check the efficacy of test. Reaction was completed in 1 hr and 10 min at temperatures 600C. It was also tested in hot water bath on same temperature. Results were same in both techniques. Results showed that LAMP was more sensitive ...

Ayesha Bibi*, Musharaf Ahmad and Shaukat Hussain 

...g commercially available kit i.e. protinase-K (sigma). 27 out of 34 isolates were confirmed as Cmm having 614bp band. Black nightshade (Solanum nigrum L) was the only weed from which pathogen was consistently isolated. From Northern KP, a total of nine weeds including; Solanum nigrum L., Solanum americanum Mill., Solanum Sarrachoides Sedntner, Amaranthus blitoides S. Wats, Amaranthus albus L., Lactuca serriola L., Amaranthus retroflexus L., Malva parviflora L....

Sana Qanber Abbasi1*, Ejaz Ahmed2 and Rabia Sattar1 

...LISA using Human pro BNP kit. Patients’ history and recorded blood pressure was documented on a questionnaire. The results were analyzed using SPSS 21. One way ANOVA and Independent sample T test were applied to compare the proBNP levels. A P-value of < 0.05 was taken as significant. Serum pro BNP levels were raised in migraine patients and also in hypertensive patients with migraine (p-value 0.00). Serum pro BNP levels were found to be raised in both...
Hamayun Khan1,*, Abdul Wahab1, Navaid Kazi2, Younas Muhammad1,Summaya Kazi3, Salman Kazi4, Azmatullah Khan1, Ikramullah Khan5 and Shakoor Ahmad1
... commercially accessible kits for biochemical markers that includes glucose, total protein, albumin, creatinine, triglycerides, blood urea nitrogen (BUN), alanine transaminase (ALT) and aspartate transaminase (AST). The current study demonstrated the ascertained value in amniotic fluid for glucose, total protein and albumin at day 18, 25 and 28 was 42.9±0.80, 37.4±0.90, 36.5±0.88mg/dl; 17.9±0.30, 16.1±0.22, 13.9±0.34g/...
Jia-kui Li1,2,*, Kun Li2, Zhao-qing Han2, Hui Zhang2, Xiao-qiang Wang2, Hou-qiang Luo2, Yan-fang Lan2 and Gang Qiu1,2
...unosorbent assay (ELISA) kit to test the prevalence of BVDV(Bovine Viral Diarrhea Virus) in yaks in Hongyuan, Sichuan province, a cold climate and high altitude area of China. Totally, 516 yak serum samples were randomly got during 2012 to 2014. BVDV was found highly prevalent among the adult yaks (51.1%; 95% CI: 46.5-55.6) and calves (60.5%; 95% CI: 43.4-76.0), respectively. The prevalence of BVDV infection in 2012 to 2014 was 45.3% (95% CI: 38.6%-52.1%); 56....
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...growth curve using CCK-8 kit on ELIASA. CAFSCs are induced to neuron and epithelium (epiblast), adipocyte (mesoblast), hepatocyte (entoblast).
...
Sana Rafique1, Hina Saqib1, Khushi Muhammad2, Nazia Akbar2, Abdul Rauf3, Muzafar Shah4,*
...n using Rapid Diagnostic kit for Dengue virus (DENV) i.e. SD BIOLINE Dengue IgG/IgM. Data was obtained from patient’s record, filled forms and through questionnaire. Total 1332 blood samples were collected from 3 tehsils of District Mansehra. Out of which, 725 were found positive for dengue fever infection. Out of 725 positive cases, 410 (56.5%) were males and 315 (43%) were female’s patients. The high rate of DENV infection was found in the...
Qudsia Yousafi1,*, Muhammad Afzal2, Muhammad Aslam3 and Allah Ditta Abid4
...uring 2013 in Sahiwal, Pakitan using brinjal variety, Nirala. The experiment was laid out in Randomized Complete Block Design with four treatments each repeated three times. The treatments were T1= removal of infested shoots, T2= Use of light trap, T3= T1+T2 and T4=control. The seedlings were transplanted on April 21, 2013 keeping plant to plant distance of 30cm. Kerosene oil lantern, hung over metallic tray filled with water, was used as light trap. Data for ...

Pakistan Journal of Zoology

October

Vol. 50, Iss. 5, Pages 1601-1998

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