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Dipak Mandal, Paramita Bhowmik, Pronobesh Halder and M. L. Chatterjee

Determination of relative toxicity and base line data of dif-ferent insecticides against cotton mealybug (Phenacoccus solenopsis Tinsley)
...ophos 40 EC (369) > spinosad 45 SC (630) > endosulfan 75 EC (950) through treated food untreated insect method and chlorpyriphos 20 EC (113) > triazophos 40 EC (217) > dichlorvos 75 EC (237) > endosulfan 75 EC (253) > spinosad 45 SC (937) through treated food and treated insect method.

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1Sitesh Chatterjee, 1Chirasree Gangopadhyay and 2Santosh Kumar Roy
...xacarb, flubendiamide, spinosad and chlorantraniliprole to test their efficacy against different rice insect-pests. The study found that whorl maggot can be managed properly in seedbed application with chlorantraniliprole granule 0.4% WW/SR in seven days prior to seedling uprooting along with spraying of indoxacarb 14.5 SC /chlorantraniliprole 18.5% SC /flubendiamide 48% SC in the main-field. The leaf folder was also managed properly by application of chlorant...
Ali Karabacak1, Senol Celik2, Adile Tatliyer3, Ismail Keskin1, Yakup Erdal Erturk4, Ecevit Eyduran5, Yasir Javed6 and Mohammad Masood Tariq7,*
... (10), Kivircik (10), Merinos (10) and Karacabey Merino (8)) reared in Konya province conditions located in the Central Anatolia Region of Turkey. For measuring the predictive performance of three algorithms in Models I and II, goodness of fit criteria (coefficient of determination (R2%), adjusted coefficient of determination (Adj.R2%), coefficient of variation (CV%), SD ratio, Root Mean Square Error (RMSE), Relative Approximation Error (...
Muhammad Aslam Farooqi1,*, Mansoor-ul-Hasan2, Sohail Akhtar1Muhammad Arshad2, Muhammad Naveed Aslam3 and Muhammad Rafay4
...rofenofos, endosulfan, spinosad and deltamethrin) in multi-floral raw honey of Apis dorsata F. collected from the cotton belt area of Punjab, Pakistan. The residues of these insecticides were extracted using ethyl acetate. Honey samples were spiked at 0.1 and 0.01 mg/kg. The mean recoveries of these insecticides in the spiked samples were 74-92% with relative standard deviation less than 20%. The residues of imidacloprid, endosulfan and deltamethrin wer...

Jawad Anwar* and Zafar Iqbal 

...a coli, Pseudomonas aeruginosa and Staphylococcus aureus) using 96 well microtitre plate method. The Ethyl acetate fraction showed maximum zone of inhibition (24.25±1.06 mm) against X. campestris and (23.15±2.12 mm) against C. michiganensis on growth nutrient broth (GNB) medium as compared to other culture media (Potato dextrose broth and yeast extract broth). Similarly maximum zone of inhibition was observed in optimized parameters like shaking ...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
...annii and P. aeruginosa, were isolated and identified using VITEK 2 compact system. Carbapenamase and metallo-β-lactamase (MBL) production by these isolates was confirmed by modified Hodge’s test and disc potentiation method, respectively. The blaNDM-1genewas amplified by PCR and sequenced. MIC of different antibiotics were determined by VITEK 2 system and antibacterial activity, MIC and MBC of manuka honey was performed...

Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali* 

...ugmented with 2 gL-1 myo-inositol and 20 gL-1 sucrose under 16/8 hours light/dark photoperiod. The study confirmed that embryogenic type II calli obtained from half of coleoptilar node of maize seedling is fully capable to regenerate into whole plant under specific chemical and environmental conditions.

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Rana Fartab Shoukat1, Shoaib Freed1,*, Kanwar Waqas Ahmad1 and Ateeq-ur-Rehman2
...dacloprid, triazophos, spinosad, pyriproxyfen and nitrinpyrum, as larvicides against C. pipiens. Highest larval percent mortality (73.3 ± 4.7) was observed after application of Ma-11.1(LC40) + nitenpyram (LC40) mixtures under laboratory and field conditions (71.5 ± 7.4). The results showed a significant effect of binary treatments of fungi and chemical insecticides on biological parameters of C. pipiens and its...
Hafiz Azhar Ali Khan1,*, Waseem Akram2, Sumi Lee3, Shakir Manzoor1, Syed Rajab Ayub1, Khalil Ur Rehman1, Shinawar Waseem Ali1, Muhammad Bilal Chattha1 and Sumaira Maqsood1
... the future.
...
Yijiang Liu1, Kun Li2, Hui Zhang2, Khalid Mehmood2,3, Muhammad Shahzad3, Houqiang Luo1,*, Muhammad Asif Yaseen4 and Xiong Jiang2,5,*
...amboo rat Rhizomys pruinosus, which had a problem in standing and walking in a Bamboo farm in Wenzhou, China was carried out, using its tibia. The mt genome (16,579 bp) is composed of 13 protein coding genes, 22 tRNA genes and 2 rRNA genes. The ratio of the bases of the mt genome of R. pruinosus from Wenzhou are A (32.31%), T (31.04%), C (24.77%), G (11.88%), A+T (63.35%), respectively. The multivariable...
Gulnaz Malik1, Abdul Qadir1 and Hafiz Azhar Ali Khan2,*
...ecticides viz., spinosad, emamectin benzoate, lufenuron and thiamethoxam. For this purpose, toxicities of insecticides were tested at three different temperatures 20°C, 25°C and 30°C, on whole wheat grains which was artificially infested with S. oryzae under laboratory conditions. The toxicities of spinosad, emamectin benzoate, lufenuron and thiamethoxam increased 2.65, 1.59, 1.64 and 3.00 fo...

Abdul Basit1, Muhammad Zeeshan Majeed1,4*, Sohail Ahmed2, Gohar Ali2 and Muhammad Javaid

...ticide formulations of spinosad. Refractive sheets of red, orange, yellow, green, blue, black and white were installed in M. charantia beds at three different angles (30º, 60º and 90º). Results revealed that control treatment exhibited significantly higher fruit fly infestation (60%) with minimum marketable fruit yield (374g/bed) than all other treatments. Among treatments, yellow colored refractive sheet treatments exhibited maximum fruit fly i...
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...e in Pseudomonas aeruginosa is a major barrier to successful treatment of infection. Additional and novel measures to control this pathogen are needed, along with contemporary information about antibiotic resistances that are present in isolates from different environments. In the present study 72 samples from blood, 43 from sputum, and 19 were obtained from tracheal aspirates patients suffering from chronic and acute lung infections admitted to a local...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...ii (n=32), P.aeruginosa (n=26), K. pneumoniae (n=19), E. coli (n=16), C. ferundi (n=04), P. vulgaris (n=02) and E. cloacae (n=01), were isolated from clinical samples of hospitalized patients. Out of these 100 carbapenem resistant isolates, 93 and 89 isolates were positive for cabapenemase and β-lactamase production, respectively. Notably, 3 (3.3%) of MBL producing strains harbor blaIMP gene ...

Muhammad Asam Riaz1, Javeria Choudhary1, Muhammad Zeeshan Majeed1*, Muhammad Afzal1, Sohail Ahmed2 and Muhammad Sajjad Khalil

...iological insecticide, spinosad, were performed on these strains of T. castaneum. Toxicological studies demonstrated that Fsd-, Jh- and Swl- strains exhibited higher tolerance ratio of 26.5-fold, 21-fold and 18.6-fold respectively relative to Sgd-strain against deltamethrin, whereas, these strains did not have significantly higher tolerance ratio against permethrin and spinosad except Fsd-strain with 9.5-fold tolerance ratio...

Muhammad Latif2, Muneer Abbas1*, Faisal Hafeez2 and Dilbar Hussain2 

...iz. spinetoram 120 SC, spinosad 240 SC, bifenthrin 10 EC, cypermethrin 10 EC, chlorantraniliprol 20 SC and flubendiamide 480 SC were applied on brinjal and okra against these pests with five replications. Data was recorded after 24 hrs. 72 hrs. 7 and 10 days after spray application. Results indicated that all the insecticides gave significant control of both pests up to 72 hrs. of spray application. After this pest population was started to increase. In L. orb...

Nasir Raza Zaidi1, Mian Waheed Ahmad1, Mahesh Gautam1, Riffat Mehboob2*

Risk factors for multiple sclerosis in Pakistani population- A crosssectional study
...ing,
and hypovitaminosis D as frequently associated with MS.

...

 Muhammad Moosa Abro1, Ali Murtaza Dharejo2, Muhammad Munif Khan2, Nadir Ali Birmani2, Saima Naz2*

Description of first record of Petasiger exaeretus Dietz, 1909 (Trematoda: Echinostomatidae) in avian host from Pakistan.

Muhammad Shahzad Akbar, Muhammad Zeeshan Majeed* and Muhammad Afzal 

...ramat, pymetrozine and spinosad were least effective against O. obesus termites. Conclusively, based on the results of this study, chlorantraniliprole, chlorfenapyr, pyriproxyfen, triflumuron and indoxacarb are recommended to be incorporated in future integrated pest management programs against subterranean termites. 

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Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...8%), Pseudomonas aeruginosa (46; 30.3%), Escherichia coli (36; 23.7%), Klebsiella oxytoca (4; 2.6%), Serratia marcescens (3; 2%), Enterobacter agglomerans (2; 1.3%) and Enterobacter cloacae (2; 1.3%). Out of 103 carbapenem-resistant isolates, 58 (55.1%) were positive by DDDT and 49 (46.7%) by MHT. Most of the carbapenems MBL resistant isolates were resistant towards drugs such as cephalosporin, ceftazidime but majority...
Faiza Jabeen1,*, Bushra Muneer2 and Javed Iqbal Qazi3
... sorbitol, mannitol, Arabinose and polysaccharides like gelatin and starch. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both isolates belong to the same specie Anoxybacillus rupiensis with 99% similarity under Accession number KF254911 (JF82) and KC849452 (JF83). The DNA G + C contents were 56.854% and 56.754% for A. rupiensis JF82 and A. rupiensis JF83, respectively. The isolates grew over a wide range of temperat...
Tanzeela Riaz1, Farah Rauf Shakoori2,*, Hareem Mansoor1, Sana Khan1 and Mushtaq A. Saleem1
...amectin, abamectin and spinosad alone and in various conbinations such as abamectin:emamectin (1:1, 2:1, 3:1, 1:2 and 1:3) and abamectin:spinosad (1:1, 2:1, 3:1, 1:2 and 1:3) against two larval instars (4th and 6th) of MBDIN and Lahore populations of T. granarium. The LC50 of abamectin, emamectin and spinosad for 4th larval instars of Laho...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...ts, Enterobacter aeruginosa, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus intermedius, Xylose staphylococcus all showed different degree of inhibitory effect. The inhibitory effect of the protein monomer on gram-positive bacteria was stronger than that on gram-negative bacteria, but not on candida albicans. This novel and monomeric APP-1 has antibacteri...

Muhammad Naeem Khan* and Asad Jan 

...ris and Pseudomonas aeruginosa causing 12.6±0.54 mm and 20.6±0.53 mm zone of inhibition, respectively which was analogous to that of cefixime, used as standard drug. In case of antifungal activity MCE hindered the growth of Fusarium oxysporum effectively, causing 19.3±0.41 mm zone of inhibition, while the activity of other solvent was moderate. 

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Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...is, pyknosis, increased sinosuidal spaces, portal hypertension, glomerulosclerosis, abnormal vascular pole in liver and kidney respectively. But when turmeric powder was coadministered with BP, these damages were reversed to a great extent. So, this study has proved the protective role of turmeric against toxicity induced by BP in female albino mice.
 
Novelty Statement | Curcumin, an active component of turmeric along with ...
Barkat Ali1,2, Wasim Sajjad1,2,3, Imran Khan1, Muhammad Rafiq1, Sahib Zada1, Aamer Ali Shah1 and Fariha Hasan1,*
... and Pseudomonas aeruginosa. Seven different types of antibiotic discs were used to check the intrinsic resistance of all the isolates to various antibiotics. Interestingly, most of the isolates were found resistant while only a few were susceptible, however, the isolates Lysinibacillus spp. N40 and Brevundomonas spp. P20 showed no activity at all. Increased levels of resistance to heavy metals such as; iron, arsenic, cadmium, chromium, an...
Bibi Nazia Murtaza1, Mazhar Saeed Chaudry2, Shamaila Inayat Nadeem1, Muhammad Shahid Nadeem3 and Abdul Rauf Shakoori4,*
...p;
...

Muhammad Rehan3, Asim Aslam3, Javeria Umber4, Muti-ur-Rehman Khan3, Waqar Azeem3, Hassan Aftab5, Ahsan Anjum3, Muhammad Abid6, Abdul Hameed Khan3, Hafiz Hasnain Ayoub5, Altaf Hussain2 and Muhammad Farooq Khalid1* 

...mmune response. Hypovitaminosis D is a significant risk factor for infectious and non-infectious diseases however its exact mechanism is still unknown, but low levels of vitamin D has been detected in different diseased cases. Recent investigations have reported various cells of body exhibiting vitamin D receptors and CYP27B1 which is capable of converting inactive form vitamin D into biologically active form 1,25(OH)2D3, that important for maintaining immune ...
Yanyan Yu1,2,3, Yizhong Hu1, Qipeng Zhang1, Rongquan Zheng1,4,*, Bing Shen1, Shenshen Kong1 and Ke Li1
... the genus Quasipaa spinosa detected the phonotaxis of the female frog for the calls from different lineages. In this work, we used a single-stimulus design to analyse phonotactic response to stimulus and subsequently estimated the values of female preference functions for call duration and call rate, and recorded and analysed the properties of 756 advertisement calls from 40 males at five reproductive stages and conducted single-stimulus phonotaxis exp...
Jam Nazeer Ahmad1,2*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2*Mubasher Ahmad Malik1, Mujahid Manzoor1, Muhammad Tahir2,  Zubair Aslam4, Sumaira Maqsood5, Ishita Ahuja3 and Atle M. Bones3
...doptera litura and Spinosad (0.01 ppm) against 3rd and 4th instar larvae collected from three different geographical areas of Punjab (Pakistan). A difference in larval mortality, pupation, adult emergence and egg ecluson was observed. The higher but sub-lethal dose rate of NPV with Spinosad exhibited synergistic interaction, while the rest of the combinations were found additive in all the tested po...
Muhammad Faheem Akbar1,*, Muhammad Abdul Haq2, Imtiaz Ahmad1, Vasileva Viliana3 and Amjad Sultan1
...as convectional, while spinosad and biosal (neem compound) as bio pesticides, and were sprayed at the rates of 49.4, 642.2, 988, 35.5 and 158 g a.i. ha-1 respectively. The pesticide residues were analyzed after 0, 1, 3 and 7 days of application in the leaves and cauliflower curd using high performance liquid chromatography. First order degradation kinetics was fitted on this data and degradation rate constants and half-life were calculated. Conventi...
Farzana Rashid1, Rabia Masood1 and Mariam Faiz2*
...n K. pneumoniae, P. aeruginosa, E. coli and Acinetobacter Species was 26%, 52%, 27% and 5% respectively. Prevalence of blaNDM-1 gene was 51% identified in 14 strains of P. aeruginosa, 2 strains of A. Baumanni, 29 strains of E. coli and 18 strains of K. pneumoniae. In conclusion, high prevalence of blaNDM-1 in our strains is a serious concern. A careful use of carbapenems is important to prevent the spread of these organisms....
Meriem Msaad Guerfali1,*, Heitham Hamden1, Salma Fadhl1, Wafa Djobbi1, Lotfi Sillini1, Wafa Marzouki1 and Mohammed Ammar2
...uce the application of spinosad that was also reported to cause resistance under extensive use. The aim of this work was to monitor the susceptibility to malathion after prolonged use in wild caught tunisian Ceratitis as well as to spinosad after a survey carried out with farmers and to give precise informations about treatment mana...
Asim Faraz*
... was kari (Capparis spinosa), dhaman (Cenchrus ciliaris) and kikar (Acacia nilotica) among bushes, grasses and trees, respectively for male and female calves in EMS.
...
Ambrin1*, Ghulam Dastagir1, Jehan Bakht2 and Muhammad Adil1
...nia and Pseudomonas aeruginosa was shown by ethyl acetate and ethanolic extracts of O. biennis. Lowest MIC value was noted for ethyl acetate and ethanolic extracts of O. biennis against Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhi and Shigella flexneri. The ethyl acetate and ethanolic extracts of Z. mauritiana var. spontanea showed good antifungal activity against Aspergillus niger, Penici...

Asmaa Abdallah Darwish 

...on: antioxidant hypovitaminosis partially participate in different ovine diseases pathogenesis and complications and their supplement for healthy and diseased animals is strongly recommended.  

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Sohail Ahmed1,*, Muhammad Waseem Saleem1, Abid Ali1, Muhammad Shahid Nisar2, Rashad Rasool Khan1 and Abdul Rashid3
... Extract (NSKE) 5% and spinosad 240 SL (Tracer) were integrated in all possible combinations, thus, making a total of 15 treatments including separate application of each above and a control where none was applied except water only. Results showed significant difference amongst treatments (p <0.05). Application of spinosad alone and in combination with other control methods proved to be the most effective treatment...
Roni Koneri1*, Meis Nangoy2 and Pience V. Maabuat1
...ance was Orthetrum pruinosum, followed by Libellago xanthocyana. The highest dragonfly species abundance was found in the plantation land, while the lowest was found in the primary forest or around the waterfall. The highest dragonfly richness index, species diversity index (H’), and species evenness index were found in the secondary forest, followed by the primary forest. The diversity of dragonflies at the observation site was influenced ...

Abdiaziz Idiris Mohamud1, Yonis Abukar Mohamed2, Osman Sheikh Ali Jama3, Pravin Mishra4* and Mohamed Idiris Mohamed5 

Prevalence and Major Pathogens Associated with Clinical and Subclinical Mastitis in Dairy Camel (Camelus dromedarius) in Benadir Region of Somalia
...5%) and Pseudomonas aeruginosa (1.85%). The prevalence of camel mastitis in the study area was significantly low this might be because of the environmental factors as well as the hygienic managements of the farms. Therefore, implementation of integrated approaches has great importance in the study sites for the avoidance and management of mastitis hence minimizing economic loss and prevents significant public health risks.

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Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo

The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
... fluorescens and P. aeruginosa to manage M. incognita was utilized. Many genes expressed in nematode feeding cells or the regulatory regions that control these genes have been isolated. Transproteins toxic to different plant parasitic nematodes can be expressed into tissues and cells feed upon by nematodes. Transgenic products with a potential to interefere with nematode physiology such as digestive enzymes or structural proteins of the intestine are considere...

Muhammad Yasir*, Mansoor ul Hasan, Muhammad Sagheer, Amer Rasul, Rameesha Amjad Ali and Habib ur Rehman

Evaluation of Spinosad Applied to Grain Commodities for the Control of Stored Product Insect Pests
...e residual efficacy of spinosad was assessed by exposing the Oryzeaphilus surinamensis, Tribolium castaneum and Trogoderma granarium to the treated commodities (wheat, maize, rice and oats) at concentrations of 0.25, 0.50 and 1 mg Kg-1 under laboratory conditions maintained at 28 ± 2oC, 65 ± 5% RH and continuous darkness. Seven bioassays were conducted by releasing the insects on treated commodities after different post treatment periods (0,1, 2,...
Amjad Ali, Kafeel Ahmad*and Shaista Rahat
...ll;">Pseudomonas aeruginosa is an opportunistic pathogen and the leading cause of nosocomial infection worldwide. This bacterium produces enzymes known as extended spectrum β-lactamases which render broad spectrum cephalosporins and penicillins inactive. This study reports antibiotic susceptibility pattern, multiple antibiotic resistance (MAR) index and prevalence of extended spectrum β-lactamases among clinical isolates of P. aerug

Muhammad Ibrahim Kubar1, Fahad Nazir Khoso1*, Imran Khatri1, Niaz Hussain Khuhro2 and Arfan Ahmed Gilal1

...ntreated control, T2 = Spinosad (Tracer), T3= Cue-lure, T4= Protein hydrolysate, T5 = Trybliographa daci. Furthermore, combinations of these treatments were designed to find out the most effective combination. The results revealed that among the solo treatments, the plots sprayed with chemical (T2 = Tracer) had least number of infested fruits (29.75±2.69) while in combine treatments, the most effective treatment was T16 (Tracer + Protein hydrolysate + c...
Waqar Ali1,3*, Muhammad Yaqoob2, Abida Arshad1, Guifu Dai3,Safir Ullah Khan1, Muhammad Arshad Malik4, Imran Ullah1 and Ihsan Ullah1
...ies, Pseudomonas aeruginosa (15.2%), Salmonella (13.5%) and Klebsiella pneumonia (8.4%). Staphylococcus aureus was found most prevalent pathogen that causes mastitis in goats. The overall prevalence of goat mastitis was recorded (11.67%). Risk factor like age, lactation stage, parity number and unhygienic condition showed statistically significant association with the prevalence of mastitis in beetal goats. Antibiotics sensitiv...
Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1
Qazi Muhammad Noman1, Farhan Mahmood Shah1, Khalid Mahmood2 and Muhammad Razaq1*
...vyol group) and static Spinosad® (spinosad 2% and methyl eugenol 51%, target group) in mango and citrus orchards at different locations in Multan, Southern Punjab, Pakistan. Bactrocera zonata (Saunders) was the most abundant fruit fly species in this study. Our results indicated a positive and significant correlation between the species of fruit flies and mean temperature in mango and citrus orchards at...

Muhammad Usman Hanif, Abu Bakar Muhammad Raza, Muhammad Zeeshan Majeed*, Muhammad Arshad, Muhammad Irfan Ullah

...ectin benzoate 1.9 EC, spinosad 240 EC and lufenuron 50 EC) and the aqueous extracts of three local plant species (i.e. Eucalyptus camaldulensis Dehn., Citrus limon L. and Azadirachta indica A. Juss) against the grubs and adults of E. vigintioctopunctata. Three concentrations of each synthetic insecticide (i.e. 2.5, 2 and 1.5%) and botanical extract (i.e. 10, 7.5 and 5%) were bioassayed using standard leaf-dip method. Results revealed that sp

Adel M. Abdelrahman1, Sahar R. Mohamed1, Soliman M. Soliman2, Sherif Marouf3* 

...egative Pseudomonas aeruginosa is harbor multidrug antimicrobial resistance of camel has serious consequences for human health, so, this study aimed to characterized of P. aeruginosa especially extended spectrum β-lactamases (ESBL) producing one; phenotypic and genotypic. The isolates of P. aeruginosa were confirmed biochemically by a Vitek 2 compact system (bioMe´rieux). Suspec...

Stephen Chijioke Emencheta1, Chinelo Charity Eze1, Anthony Amaechi Attama2, Damian Ejike Agbo1 and Ebele Benedette Onuigbo1*

...med the Pseudomonas aeruginosa isolates. The bacterial isolates were sensitive to only ciprofloxacin. The Multi Antibiotic Resistance Indexes (MARI) of the isolated strains were greater than 0.8. The formation of plaques (clear zone) on the lawn of Pseudomonas spp confirmed lytic action. The bacteriophage showed plaques on Pseudomonas sp. isolated from other sites also. The isolation of Pseudomonas aeruginosaphages from resi...
Yang Yang1,2, Ji Shao1,2, Mingxu Zhou3,4, Qiangde Duan1,2, Xinyi Zhang1,2 and Guoqiang Zhu1,2*

...> of Pseudomonas aeruginosa which is responsible for the synthesis of long side-chain AHL (3OC12-HSL), and gene yenI of Yersinia enterocolitica which is responsible for the synthesis of short side-chain AHL (C6-HSL), were selected and transformed into ETEC strain C83902, respectively, and endowed with the ability of endogenous synthesis of 3OC12-HSL and C6-HSL. At the same time, 200 μmol/L 3OC12-HSL and C6-HSL were added into the cultur...

Neda Amani1, Mehrdad Shariati1, Rahim Ahmadi2,3*, Mokhtar Mokhtari1 and Saeed Khatamsaz1

Kanakuntla Sandhyarani*, Dhoppalapudi Madhuri, Yadala Ravikumar 

...ein (> 30%), Hypovitaminosis A, Hypervitaminosis D3, dehydration, high sodium carbonate, Copper sulfate, mycotoxins in feed causes renal failure leads to gout. The managemental practices involves high brooding temperature thereby reducing the water intake and hence increasing chances of development of gout. In addition, products used on a routine basis and result into toxicity includes antibiotics, anticoccidials, manufac...

Mariam Arain1, Asghar Ali Kamboh1* and Muhammad Javed Arshed2

...owever, Pseudomonas aeruginosa and Staphylococcus aureus were significantly higher (p<0.05) in control group goats compared to goats in supplemented group. Significantly higher (p<0.05) IgG concentration was found in Se supplemented goats as compared to control group. The bacterial species produced weak positive biofilm formation (p<0.05) in Se supplemented goats as compared to control group. The hematological indicators revealed that the values of he...

Tiyyabah Khan1, Hafiz Azhar Ali Khan1*, Muhammad Rizwan Khan1, Muhammad Umer1, Adnan Akhter1 and Waseem Akram2

...rational insecticides (spinosad, thiamethoxam, indoxacarb and imidacloprid) against Pakistani strains of Cryptolestes ferrugineus (rusty grain beetle) and Tribolium castaneum (red flour beetle), and tested whether insects’ mortality could control fungal contamination in stored grains or not? Bioassays were performed on wheat grains artificially infested with C. ferrugineus or T. castaneum along with spores of Aspergillus flavus or A. parasiticus. The res...

Wei Cui1, Yanwei Du1, Lijuan Jiang1, Yan Wei1, Yuguo Li1, Wenfeng Zhang1* and Ling Zhang2*

...sphorylated phosphatidyl inositol 3- kinase), p-AKT (phosphorylated protein kinase B) and Bcl-2 decreased in model and HG group, while the expression of Bax, Caspase-3, and Cleaved Caspase-3 increased. After BHD intervention, p-PI3K, p-AKT, Bcl-2 expression increased, while Bax, Caspase-3 and Cleaved Caspase-3 expression decreased. In conclusion, BHD may play a protective role on diabetic hippocampal neurons by regulating mitochondria related apoptosis protein...

Ghulam Murtaza1,6*, Muhammad Irfan Fareed2, Noshaba Munawar3, Muhammad Nadeem Khan4, Gul-E- Zahra5, Taskeen Ahmad6, Muhammad Daud6 and Taqi Raza6

...ifferent insecticides (Spinosad 100%EC, chlorfenapyr 36%SC, pyriproxyfen 10% EC, matrine 0.5 AS and imidacloprid 70% WDG) against Aedes aegypti larval mortality under laboratory conditions. Result indicated that Spinosad (77.32% mortality) and pyriproxyfen (66.28% mortality) are more toxic to A. aegypti larvae as compared to matrine, chlorfenapyr and imidacloprid after 72-h exposure. While minimum mortality was recorded afte...

Mehwish Saleem Khan* and Sumama Farooq

...owed by Pseudomonas aeruginosa (13/60), then Klebsiella (12/60) and minimum number was of Proteus spp. (5/60). Family Enterobacteriaceae and Pseudomonas aeruginosa are responsible for infections of brain, eyes, ears, digestive tract, respiratory tract and urinary tract. It has become difficult to treat these infections as these microbes have become resistant to variety of antibiotics. Due to multidrug resistance only few cho...

Muhammad Ajmal Khan1, Muhammad Yahya2*, Ali Hazrat2, Javed Khan3, Saeed Jan4, Tabinda Nowsheen2 and Inam Ullah5

...phi, E. coli and P. aeruginosa, with ZOI observed was 22, 18 and 20 mm, respectively. Antioxidant activity evaluated by using different concentrations of methanolic extracts showed good activity at 250 μL through DPPH, which depicted the vibrancy in phenolic, flavonoids and terpenoids contents. This insightful study highlighted the potential usage of methanolic leaves extract of T. camphoratum as a broad-spectrum antibacterial agent and an antioxidant sourc...

Ochieng Onunga George*, Owuor Ndonga Millicent Florence, Mario Kollenberg  

...ed were E. coli, P. aeruginosa, K. pneumoniae, E. faecalis, and S. dysenteriae. Bacterial concentrations cfu/100ml of abattoir effluents ranged from 3.17 × 106 of TC. 3.94x104 of FC, 2.84x104 of E. faecalis, 8.65x104 of E. coli, and BOD of 828.04mg/l.Fungi isolated were., Aspergillus niger, Fusarium oxysporum, Penicillium spp, Aspergillus fumigatus, Saccharomyces cerevisiae, Aspergillus flavus. Parasites isolated from samples of effluent and water were B...

Hanan Saad El-Samahy, Amani Abd El-Naby Hafez, Mohamed Talat Ragab, Disouky Mohamed Mourad*  

...a), and Pseudomonas aeruginosa (P. aeruginosa) . The most prevalent detected bacteria were E coli (13) followed by M. gallisepticum (12), ORT (12) and M. meleagridis (7). Two farms reported single infection, one with M. gallisepticum and one with E. coli. Only one farm had no infection while mixed infection recorded in 14 farms. P. multocida and P. aeruginosa were not detected. It was conc...

Bilal Dik1, Saima Naz2*, Muhammad Sohail Sajid3,4 

.... nisus, and Circus aeruginosus; 2 each on Pernis apivorus, Ci. pygargus and Milvus migrans; and one each on Circaetus gallicus, Hieraaetus pennatus, Aq. chrysaetos and Aq. gentilis. Circus cyaneus, Ci. macrourus, Clanga clanga and Accipiter brevipes were not found infested with the louse. There are some lice species reported for the first time in Turkey, put new records for the hosts and the country on record. Many Turkish birds are, however, yet to be screen...

Syed Ishfaq Ali Shah1,3, Azaz Ahmad2 and Wanzhi Cai1,*

...ae. Acanthaspis quinquespinosa (Fabricius, 1781), is a common species in Pakistan. Due to its variable color patterns, its taxonomic distinction from Acanthaspis flavipes Stål, 1855 has been confounded. The genitalia in Reduviidae are commonly used for species identification and in present studies, because of identical genitalia, A. flavipes Stål, 1855 stat. restit. is restored as a junior synonym of A. quinquespinos...

Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2

...lar L. and Nicotiana glutinosa for virus propagation. Fresh wood cuttings (4-6 nodes) were collected from naturally infected fig plants. Healthy fig plants were grafting-inoculated with chip budding of infected fig plants and kept in a greenhouse. Symptoms were confirmed by DAS-ELISA. Strips of infected and healthy N. glutinosa leaves were viewed under light microscope. Extracted infectious sap of N. glut

Reda Hassan*, Bahaa Abou-shehema, Sherif Zayed, Micheal Gorgy, Shama Morsy, El-Sayed Abu El-Hassan, Mahmoud El-Gbaly, Hanaa Basuony, Ebtehal Hassan 

...rated sodium calcium aluminosilicate (HSCAS) (0.5%) and Saccharomyces cerevisiae (SC) (0.1%) on the preclusion of aflatoxin-B1 (AFB1) toxicity in broiler chicken during 1 – 42 days of age. Eight hundred broiler chicks were arbitrarily assigned for 8 treatments of 5 replicates (each contained 20 chicks). On the other hand, chickens were offered the basal dietas Negative Control (NC, group 1); the other three groups 2, 3, and 4 were fed NC supplemented wit...

Uzma Hameed1*, Aneela Liaqat1, Muhammad Asjad Khan2, Ikram-Ul-Haq1 and Maham Aslam1

..., starch, galactose, arabinose and sucrose) best results were obtained with soluble starch. Alpha-amylase production is sensitive to the supply of nitrogen sources. In this context, both inorganic and organic nitrogen sources, were evaluated. Optimal enzyme production was obtained with diluent supplemented with NaNO3 (0.25%) and peptone (0.25%) as inorganic and organic sources, respectively. Among the different metal ions evaluated, Ca2+ gave the maximum activ...

Öznur Özil*, Öznur Diler, Mevlüt Nazıroğlu, Aşkın Atabay 

... histopathology of the Clinostomum complanatum infection in Garra rufa used in the health and beauty industries in foot spas for ichthyotherapy. In total of 25 examined fish specimens 56% were infected with the metacercariae of C. complanatum. The mean intensity of the infection was 27.14 cysts per host, varying between 1-75 cysts. The parasites were determined encysted in the base of the fins, muscle, inner wall of the operculum, gill arches, lips, upper jaw...

Nadia A Eltablawy1, Ibrahim El Tantawy El Sayed2, Hamed Mohamed Abdel Barry2, Marwa A. Ibrahim3*, Maha Nageib Ahmed Serag ElDein2 

A.W. Amin†, A.A. Anter, A.H. Ashoub* and A.S. El-Nuby*

...eus and Pseudomonas aeruginosa recovered
from tomato rhizosphere and tested for their ability to induce systemic resistance or bio-control agent against
Meloidogyne incognita in tomato under greenhouse condition. Results showed that all tested bacterial strains
showed significant reduction in nematode development and reproduction. The most effective strains were M.
methanica, B. cereus, B. brevis and O. proteus. ...

Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4

...ion zone against P. aeruginosa, with acetone extract. The same extract seems inactive against K. pneumoniae as compared to control. When tested on S. aureus, chloroform showed a 10 mm inhibition zone while it was inactive against all other experienced bacteria. Ethyl acetate extract gave an inhibition zone of 10 mm against S. aureus, B. subtilis, and P. aeruginosa while it was inactive against the rest of the bacteria. Using...

B. Archana and R. Saxena

...lants viz., Amaranthus spinosus,Chenopodium album, Catharanthus roseus, Solanum nigrum and Ocimum sanctum were investigated for their nematicidal activity against second stage juvenile of Meloidogyne incognita by in vitro technique. Aqueous extract of all plant roots exhibited nematicidal activity. LC50 values calculated as 1024 ppm for C. roseus, 1867 ppm for S. nigrum, 1968 ppm for A. spinosus, 3428 ppm for C. album and 96...

Hidayat Ullah1, Sadia Tabassum1, Sultan Ayaz2, Shumaila Noreen1, Atta Ur Rehman1, Naveed Akhtar3, Muhsin Ali3, Zaib Ullah3* and Sajid Mahmood1,4

...oides, Haemaphysalis bispinosa were identified from goats and three species Hyalomma marginatum, Hyalomma excavatum, Rhipicephalus microplus from sheep. Goat breed wise infestation rate was recorded in Kaghani, Kamori, Sindhi, Lehri, Barbari, Beetal and Dera Din Panah. In sheep breeds infestation rate was recorded in Gauder, Balkhi Afghani and Rambouillet. On basis of ITS2 gene and 16SrRNA phylogenetic analysis of Rhipicephalus haemaphysaloides and Rhipicephal...

Noor Rahmawati, Alfi Rumidatul* and Sopandi Sunarya

...oli and Pseudomonas aeruginosa were used. The experiment showed that the addition of yeast and sengon wood to PDB medium increased the growth and production of bioactive compounds extracted from fungal cultures. A fungal endophyte extract in ethyl acetate from all media used produced antioxidant compounds with high activity. Antibacterial activity was obtained from the extract of fungal cultures grown on PDB plus Sengon wood floor on day 6 for P. aerug

Hanaa M. Abdelkhalek1, Hanan E. Nagib1, Randa S. Elias1, Saad S. Mansour1, Walid S. Mousa2*

... herds. Pseudomonas aeruginosa (P. aeruginosa) is opportunistic pathogens involved in veterinary disorders including clinical mastitis in buffaloes. This study aimed to investigate the antibiogram pattern and synergistic effect of amikacin and norfloxacin against resistant P. aeruginosa isolates from mastitis origin. In addition, detection of some virulence and antibiotics resistance genes...

A. S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik2; Nashwa M. A. Abd El Mohsen1; and H. M. Abdelmaksoud1

... cv. Cara. Nicotiana glutinosa L. Nicotiana benthamiana gave systemic infection only. On the other hand. systemic symptomless infection had occurred with N. tabacum L. cvs. White Burley and Xanthine. Whereas PAMV didn't infect Ch. umaranticolor Cost. Reyn and D. metel L. The virus was transmitted by aphids (M persicae) in a non-persistent manner only when the aphids fed firstly on PVY N source for 10 min. This indicated a kind of synergistic effect between the...
Saiwa N. Zein
...itaceae. Ebenaceae. Leguminosae and Solanaceae. The identity of the virus to TRV was confirmed by mode of transmission. particle morphology and serological typing. Examination or the purified virus preparation 1» electron microscopy revealed rod-purify the Egyptian isolate of TRV. The yield of the virus was 2.6-5.7 mg/ 100 g tissue of Nicotiona rustica plants. The polyclonal antibodies raised against the Egyptian isolate of TRV had a specific titer of I:...

Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3 

...0%) and Pseudomonas aeruginosa (1.5% and 3.3%). Among the recovered E. coli serotypes, the serotype O111:K58 (B4) was the most prevalent (38.2%). An inactivated polyvalent autogenous mastitis vaccine was prepared from the most encountered bacterial pathogens, namely, E. coli, S. aureus, Str. agalactiae and Str. dysgalactiae. The selected strains for vaccine preparation were inactivated with a predetermined minimal lethal dose of gamma radiation (4krad kGy/min...

Altaf Ur Rahman1, Muhammad Ajmal Khan2*, Ali Hazrat3, Awais Farid4, Muhammad Yahya3* and Inam Ullah5

...rain in order of P. aeruginosa > E. coli > S. typhi > MRSA comparable to vancomycin. The highest percent mean growth inhibition (MGI) was observed against P. aeruginosa of 90.5±4.3 compared to vancomycin (61.26±6.31). The Zone of Inhibition (ZOI) noted against P. aeruginosa was 20 mm compared to vancomycin (17 mm). The quantitative test antioxidant activity of the me...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
...formis, Pseudomonas aeruginosa and Bacillus subtilis). Secondary screening using ethyl acetate extracts also showed significant ZI in the range of 06 – 14.0 mm (P ≤ 0.05) against these pathogens thus confirming their bioactive potential. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of screened Actinomycetes was in the range of 1.3 - 3.5 mgmL-1 and 1.9 – 4.0 mgmL-1 of bacterial biomass, respectively. Thr...

Mohammad Bani Ismail

...y. Zizyphus jujuba var spinosa seeds contain SPN, a C-glycoside flavonoid used in traditional medicine. A growing body of evidence indicates they have pharmacological potential against a wide range of diseases. Spinosin (SPN), however, has not been examined in relation to doxorubicin (Dox) induced cardiac injuries. To find out if SPN can reverse Dox-induced cardiomyopathies, we conducted this study. Results showed that Dox t...

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1 

...53.3%), Pseudomonas aeruginosa (40% & 35%), Staphylococcus aureus (33.3% & 25%), Streptococcus agalactiae (21.6% & 18.3%), Klebsiella pneumoniae (15% & 13.3%), Staphylococcus epidermidis (13.3% & 10%), Klebsiella oxytoca (10% & 8.3%), and Streptococcus pyogenes (6.6% & 1.6%) in market and pond fish samples respectively. According to antibiotic susceptibility tests, among 11 tested antimicrobials, S. agalactiae and S. pyogenes were...

Iftikhar Ahmad1, Tahir Saeed1, Umair Faheem2*, Qaisar Abbas2, Muhammad Saleem Akhtar Khan3, Mussurrat Hussain2, Tanveer Ahmad4, Gulzar Akhtar4, Asifa Hameed5, Muhammad Hasnain6 and Muhammad Jamil7

...z., imidacloprid 20Sl, Spinosad 240SC, Spintoram 120SC, Chlophenapyre, Imidacloprid+fipronil and abamectin were applied. Thrips population was observed on jasmine flower before application of insecticides and thrips mortality data was noted after 24, 72 and 168 hours of insecticides spray. The maximum mortality of thrips was recorded in Imidacloprid+fipronil (68.45%) and Spinosad (65.45%) after 24 hours of insecticides spray...
Xiao-Wei Huang1,2, Mei-Li Liu1, Jin-Ji Wang1, Yue-Xin Liu3, Zhe Lin1, Chun-Shu Rong4* and Ji-Xiang Ren4*
...ression levels of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt) and mammalian target of rapamycin (mTOR) in the hippocampus were detected through western blotting. In the results, VAP contained 17 kinds of AA, including 7 essential AA, with a content of 394.08 g/kg, the total AA content was 831.55 g/kg. In the OFT, the PIR, VAPH and VAPL groups showed significant decreases in slient period, significant increases in exercise time and behavior path, a...
Nassarl , Entsar A.; El-Dougdoug , Kh. A.; Osmanl , M.E; Dawoud3, Rehab
A. and Kinawy l , Aliaa H.*
...chnique on Nicotiana gultinosa. The induced antiserum for the isolated virus had a titer 1\1024. 600 bp DNA fragments from the coat protein gene (CP) of TMV—Ch—EG was amplified with Rt-PCR technique. Phylogenetic analysis of the TMV-Ch-EG/CP- gene showed 89% nucleotide sequence homology with other published strains of TMV in GenBank and 81% amino acid sequence homology. Tissue culture approach was used to pemit the recovery of TMV-Ch-free microprop...
Mazharul A. M. 1, Md. S. Sarwar ,M. Nurullah 2, Keshab K. Adhikary I and M. Rahmatullah 1 
 
...erodendrum viscosum and Tinospora cordifolia were used for treatment of viral fevers. Stemona tuberosa was used in case of measles. Moringa oleifera and Azadirachta indica were used to treat chicken pox. Three plants, namely Cyathula prostrata, Amaranthus spinosus. and Calotropis procera were used to treat patients with rabies.
...

Amina A. II. and M.K. Amin

...fecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of plaques (turbid and clear) have been observed. The average number of turbid plaques was 2.52 x 109 pfu/ml whereas of clear plaques was 9.96 x 107 pfu/n) l. The phage was able to lysis 13 out of 20 strains of Pseudomonas aerug<...

Divanildo Outor-Monteiro1,2,3*, José António Silva2,3,4, José Luís Mourão1,2,3, Victor Pinheiro1,2,3

... along with increased luminosity and reduced redness. In conclusion, production systems exert influence on specific carcass and meat characteristics.
 
Keywords | Rabbit, Housing system, Slaughter-age, Carcass, Meat
...

Tabinda Nowsheen1, Sayed Wadood Ali Shah2, Ali Hazrat1*, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar4 and Muhammad Ajmal Khan3

...5µg/ml for P. aeruginosa, (gram negative), and B. subtilis (gram positive) that is nearly similar to the activity of ciprofloxacin (standard). The methanolic leaves extract of A. maritime displayed the highest scavenging activity (78.09µg/ml) in DPPH, while stem methanolic extract showed 61.81 µg/ml activities. In ABTS the highest activity (84.13µg/ml) was observed on leaves extract and lowest (69.18µg/ml) on stem extract. Our cur...
Rehan Ahmed Siddiqui1,2*, Shabana Usman Simjee2,3, Nurul Kabir4, Muhammad Ateeq3,5, Kevin Joseph Jerome Borges6, Muhammad Raza Shah3 and Rahman M. Hafizur2
Pratap A. Divekar1,2*, Sampat K Patel2, Guru Pirasanna Pandi G3, Manimurugan C2,4, Vikas Singh2 and Jagdish Singh1
...nsect pest population. Spinosad, emamectin benzoate, indoxacarb and chlorantraniliprole were also the found best treatments in controlling DBM and CB. No phytotoxic symptoms were observed in any treatment after spray application. Chlorpyriphos, deltamethrin, lambda-cyhalothrin and flubendiamide were found adverse to natural enemies. Thus, spinetoram, spinosad, emamectin benzoate, indoxacarb and chlorantraniliprole are recomm...

Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee

...div>Keywords | Hypervitaminosis D, Chromosome gap, Histopathological, Cytogenetic effects, Vitamin D, Median lethal dose, Mitotic index, Rats, Female rats.
...

IRFANA IQBAL1*, PAKEEZA TANWEER1, FARKHANDA MANZOOR1, MUHAMMAD NAUMAN AFTAB2, AFSHAN KALEEM3, ROHEENA ABDULLAH3, ASMA ZAFAR4 & MEHWISH IQTEDAR3

...re identified as P. aeruginosa, E.coli, K. pneumoniae and S. aureus. The original bacterial inoculum was serially diluted (adjusted to 1.5 X 106 CFU) and spread on the nutrient agar plates. Whattmann filter paper discs were soaked in three different concentrations (50%, 70% & 90%) of each of the three unifloral honey samples for 48 hrs. The filter discs were placed on the agar plates seeded with the individual bacteria. Solitary effect of antibiotic discs ...

HANNANA MARYAM, SANA MAQSOOD & SIKANDER ALI*

...aureus, Pseudomonas aeruginosa and Azotobacter sp. It was concluded that Brevibacillus sp. possesses strong potential for laterosporulin biosynthesis. Further manipulation of physical and nutritional parameters improved its biosynthesis in liquid culture.

...

Biologia (Lahore)

December

Vol.65, Iss. 2

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