Xingdong Yang and Lijuan Yuan
...esent through deliberate inoculation with a particular virus strain or bacterial species and being maintained free of unwanted microbiota in sterile isolators. The sterile surgical derivation and gnotobiotic status of the pigs allow studies of the infection, disease and immune responses caused by a specific pathogen in the absence of interfering maternal antibodies, other maternal immune regulators, and intestinal and environmental microbes. Due to the similar...
Rashid Saleem1*, Zammurad Iqbal Ahmad2, Muhammad Abbas Anees2, Abdul Razzaq3 and Ashiq Saleem1
...h (PK) (80-60 kg ha-1) + inoculation, T3, (120-80-60 NPK kg ha-1); T4, poultry manure (PM) @ 15 t ha-1 and half PM+ half PK+ inoculation, half poultry manure (7.5 t ha-1) + half PK (40-30 kg ha-1) + inoculation. According to statistical analysis, data revealed that plots treated with PK + inoculation with Rhizobium gave 33% higher mungbean seed yield ove...
Aqeel Ahmad, Zammurad Iqbal Ahmed, Irfan Aziz*
...tons ha-1 and inoculation by phosphorus mobilizing microorganisms 2.5 packets ha-1, in addition to control were evaluated under lentil-sorghum and lentil-mungbean cropping patterns. Fertilizer treatments were only applied to lentil while their residual effect was studied on sorghum and mungbean. The results revealed that net benefits and marginal rate of return from NPK were the highest mainly due to high lentil yield and poultry manure w...
Muhammad Arif Zafar1, Murtaz-ul-Hasan1*, Aayesha Riaz1, Asim Shamim2, Muhammad Farooq Iqbal1 and Arfan Yousaf
E-mail | murtazhassan@yahoo.com
...roup C were treated with inoculation of autogenous vaccine supplemented with immune-modulators (Levamisole and Vitamin E+Selenium). The animals treated with autogenous vaccine along with immune-modulators showed complete regression of the papillomas compared animals, which were treated with autogenous vaccine alone. The partial excision of the papillomas showed no obvious differences. In conclusion, autogenous vaccine synergistically affected the imm...
Sayeda Sarah and Muhammad Ibrar
...mycorrhizal fungal (AMF) inoculation (with and without Mycorrhiza) along with application of various levels (0%, 25%, 50% and 100%) of rock phosphate (RP) fertilizer on spores density and percent root colonization of selected sunflower (Helianthus annuus L.) hybrids in P-deficient soil. It was observed that spore density and AMF root colonization was higher in the soil of control (RP0) plants, which decreases progressively with increasing fertility level. Less...
Sadiq Hussain, Muhammad Sharif, Sarmad Khan, Fazli Wahid, Hina Nihar, Wiqar Ahmad, Imran Khan, Nadeem Haider and Tabassum Yaseen
...the effect of mycorrhiza inoculation with vermicompost on yield and P uptake of wheat. The experiment was carried out in Completely Randomized Design replicated four times during season 2014-15. Phosphorus (P) was applied at recommended level of 90 kg ha-1. Vermicompost was used as a source of P on the basis of its P concentration. The recommended dose of N @ 120 kg ha-1 was applied in the form of vermicompost and urea, while K was applied in the form of sulph...
Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5
...The relationship between inoculation dose and emergence of IJ by using both methods was non-significant. Similarly, with an increase in inoculum dose and time, invasion of host was significantly increased. There was little invasion at the 800 IJ dose even after 4 days whereas with the commercial dose (8,000), nematodes did migrate to the larvae and caused some infection after 2, 3 and 4 days. Greatest invasion took place with the highest dose (80,000) with 8 I...
Muhammad Raheel1,*, Nazir Javed2, Sajid Aleem Khan2, Hafiz Muhammad Aatif3 and Sohail Ahmed4
...s of each species. After inoculation at different temperatures, the reproductive potential of EPNs increased with increasing temperature and was found to be the best at 25oC. No EPNs species could reproduce at 5oC. S. feltiae started reproduction at 10oC, while all remaining species reproduced at 15oC or higher temperatures. Maximum numbers of IJs were recovered from H. bacteriophora. Time taken for first emergence of IJs from the host was shortest at 25oC i.e...
Matiyar Rahaman Khan1,*, Sabyasachi Pal2, Ghule Tushar Manohar2, Somnath Bhattacharyya3, Amit Singh4, Prahlad Sarkar5 and Samuel Lalliansanga6
...fruit was proved through inoculation studies and inoculated plants also produced almost similar above-ground symptoms and profuse root galling. The pathogenic potential of M. incognita on passion fruit was determined; the minimum 10 second stage juvenile (J2)/200 cc soil can cause reduction of plant growth parameters and highest reproduction (R=87.27).
... 
Response of Spring and Autumn Sunflower to Chemical and Bio-Fertilizers for Yield and Quality Traits
Muhammad Tahirand Memoona Shehzadi*
...were recorded where seed inoculation with PGPR-1 + PGPR-2 and full dose of recommended chemical was applied during both the growing seasons. Overall the performance of sunflower was better in spring as compared to autumn season. However, it was also noted that the combination of PGPR-1 + PGPR-2 and half recommended doses of chemical fertilizer gave results as full recommended dose of chemical fertilizer alone. Therefore, PGPR enhanced the yield by reducing the...
Zia Ur Rahman1*, Syed Mehar Ali Shah1, Hidayat Ur Rahman1, Fida Mohammad1, Mian Ahmad Raza1 and Ijaz Ahmad Khan2
...ng season. On artificial inoculation with Xanthomonas oryzae pv. Oryzae, the fundamental agent of bacterial leaf blight, most of the studied rice accessions and the two commercial rice cultivars displayed susceptible reactions. Seven rice accessions viz. UoA-2, UoA-5, UoA-11, UoA-48, UoA-53, UoA-102, and UoA-123 showed moderate levels of resistance while eleven rice accessions viz. UoA-4, UoA-6, UoA-22, UoA-41, UoA-60, UoA-67, UoA-68, UoA-84, UoA-126, UoA-129 ...
Bushra Afreen, Geetesh Baghel, Akil A. Khan and Q.A. Naqvi
Virus- vector relationship of yellow vein disease of calendula and the whitefly
...iteflies not through sap inoculation. A single whitefly could transmit the virus and showed 20.55% infection and fifteen whiteflies were required for 100 percent transmission. The minimum acquisition access feeding period and minimum inoculation access feeding period for the present virus were 10min and 30min, respectively. Acquisition access feeding period and inoculation access feeding p...
A. Sajeena and T. Marimuthu
Efficacy, stability and persistence of Ganosol, a Ganoderma based fungicide against plant pathogens
...st the pathogen. The pre inoculation spray of the formulation was observed to produce 63.62% reduction in lesion height caused by the sheath blight pathogen. Two isoforms of poly phenol oxidase, PPO 1 and PPO 3 were observed to be induced only in Ganosol 10EC sprayed plants and were absent in control. The formulation was tested to have good emulsion stability. The antifungal activity of the formulation persisted up to seven days after spraying the formulation ...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
Gadeeyya G and Ratna Kumar P.K.
...mae were isolated by inoculation of parasitized weed propagules like infected leaves, stems, root and floral parts on different fungal growth media. The diagnostic characteristic features of each isolate were critically studied using relevant literature. The pathogenicity of the isolates was examined to select biocontrol agent against host weed.
...
Muhammad Mansoor1, Amanullah1, Zafar Islam2*,Sher Muhammad3, Muhammad Umair4, Mehmood Ayaz5, Asghar Ali Khan6, Muhammad Asif2 and Ya Sakina3
...2O5 and inoculation with rhizobium strain Vm M1 were optimal for its maximum yield. Inqalab Mung outclassed among all candidate lines included in National Uniform Yield Trial (NUYT)- 2007 with an average yield of 1092 kg ha-1 at 12 locations across country, with highest grain yield of 3620 kg ha-1 at Agricultual Research Station ARS, Mingora, Swat, Khyber Pakhtoon Khawa. Again in NUYT-2008, Inqalab Mung outcla...
Muhammad Tariq Saeed1*, Muhammad Ashfaq Wahid1, Muhammad Farrukh Saleem1, Mumtaz Akhtar Cheema2, Muhammad Shahid1, Abdul-Shakoor1, Abdul-Sattar3
...hydro priming, hydration inoculation and osmo priming with 0.5% urea solution. Results revealed that hydro priming, on-farm priming and hydration inoculation significantly reduced the time to 50% emergence (E50), mean emergence time (MET) and emergence index (EI) of both soybean varieties. Moreover, osmo priming had no significant effect on E50, MET and EI that was at par with control. On an average, da...
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4
...ays after single or dual inoculations and were inspected for gross lesions. Samples of the respiratory organs (trachea, lungs, and air sacs) were taken for histological analyses. All the infected subjects showed clinical signs of varying severity. Co-infected groups showed the most obvious clinical signs, associated with significant higher mortality and respiratory organ abnormalities, in comparison with the mono-infected groups (P<0.05). There was a non-si...
Muhammad Aqeel Sarwar1*, Abid Ali2, Manzoor Hussain2, Saadia3, Muhammad Khubaib Abuzar4, Ijaz Ahmad5 and Sohail Latif6
...Azospirillum seed inoculation could be a better management approach for sustainable wheat production.
...
A. R. Rattu, M. Fayyaz, I. Ahmad, M. A. Akhtar* A. A. Hakro and K. A. Khanzada**
...st along with artificial inoculation with prevalent pathotypes. These candidate lines were undergoing mandatory testing to develop varieties for irrigated and rainfed areas of Pakistan. Out of these 29 candidate lines, 9 were common to NUWYT 2003-04 and 2004-05. Three candidate lines namely, V-00125, 99B2278, 99B4012 from seeding date were found resistant to both leaf and yellow rusts and showed desirable Relative Resistance Index (RRI) during 2003-04 and 2004...
Muhammad Asim, Muhammad Asif, Muhammad Munir* and Muhammad Aslam**
... Merr.] from the time of inoculation to root-nodule initiation (192 hours after inoculation). 3H-ABA was applied to the leaves of two soybean cultivars: cv. Williams-82 and its hypernodulating mutant, NODI-3. There was a significant difference in the percent uptake of 3H-ABA between the two varieties both in inoculated and uninoculated plants. A marked difference in the percent distribution of 3H-ABA between different parts ...
Zammurad Iqbal Ahmed, Muhammad Ansar*, Ashiq Saleem**, Zafar ullah Arif*, H.I. Javed and Rashid Saleem**
IMPROVEMENT OF MASH BEAN PRODUCTION UNDER RAINFED CONDITIONS BY RHIZOBIUM INOCULATION AND LOW RATES OF STARTER NITROGEN
...s earlier in control and inoculation + 20 kg N ha as compared to all other treatments. Inoculation in combination with 50 kg N -1 ha significantly delayed crop maturity. The maximum mean average grain -1 yield (681.5 kg ha ) was augmented with the application of inoculation alone and non significant differences were noted among the varieties. Biological yield were also significantly increa...
Muhammad Arshad Ullah*, Nazir Hussain**, Helge Schmeisky*** and Muhammad Rasheed****
INOCULATION AND INTER-CROPPING OF LEGUMES IN ESTABLISHED GRASS FOR INCREASING BIOMASS OF FODDER
...nd cowpeas) coupled with inoculation was studied under rainfed conditions at National Agricultural Research Centre (NARC) Islamabad, Pakistan. Intercropping significantly increased tillering of grass. Seed inoculation of legumes also gave maximum tillers. The grass and legumes biomass without any treatment were recorded as 7.09 and -1 8.17 t ha , respectively, during two years of study. Mixed fodder -1 production increased t...
Muhammad Arshad Ullah*, Nazir Hussain**, Helge Schmeisky*** and Muhammad Rasheed****
IMPROVING FODDER QUALITY OF PANICUM GRASS THROUGH INTERCROPPING OF LEGUMES AND THEIR INOCULATION
...and 67%) with or without inoculation to investigate its fodder quality. The study clearly indicated that quality of fodder increased significantly both with the legumes and inoculation. The intercropping of Panicum grass with 67% inoculation proved the best combination. The 6-7% higher crude protein (CP) of mixed fodder was recorded from 67% intercropping in comparison to grass alone while...
Muhammad Zahid Kiani*, Tariq Sultan**, Arshad Ali**, Ghulam Qadir*** Imdad Ali Mahmood**, Tauseef Tabassam**, Muhammad Arshad Ullah** and Nasir Abbas*
EFFECT OF PGPR STRAINS ON SUNFLOWER GROWTH AND NUTRIENT CONTENTS UNDER SALINITY STRESS
...sed because of bacterial inoculation. The strain KS 7 -1 indicated the maximum phosphorus contents at 5dS m which was 313% higher as compared to control. Similarly K+ content significantly increased at all salinity levels. This study concluded that PGPR isolate KS 44 is the most effective strain followed by KS 7 among all tested strains in improving sunflower growth and P, K content under salinity stress....
Muhammad Zameer Kayani1,*, Tariq Mukhtar2 and Muhammad Arshad Hussain3
... hand, ages of plants at inoculation had negative correlations with reductions in these parameters at each inoculum density. The production of galls was found to be positively correlated with the inoculum densities and plant ages. However, rate of nematode build up decreased with an increase in inoculum density and appeared to be negatively correlated with inoculum densities and, on the contrary, was found to be positively correlated with plant ages. The resul...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...virus by embryonated egg inoculation technique. Moreover, out of 507 samples, 479 serum samples were scrutinized by enzyme linked immunosorbent essay (ELISA). Sero-prevalence of AIV among different species of wild birds was as follows; peacock (n=35; 14%), duck (n=5; 2%), migratory water fowl (n=3; 1%), pheasant (n=2; 0.8%), grey leg goose (n=1; 0.4%), turkey (n=1; 0.4%), eagle (n=1; 0.4%) and crane (n=1; 0.4%), for domesticated bird species sero-prevalence wa...
Niamatullah Kakar, Farhat Abbas, Muhammad Shafee and Tauseef Asmat*
...r microscopy and culture inoculation for the detection of Mycobacterium tuberculosis infection. Moreover, the suspected samples were further investigated by polymerase chain reaction (PCR). Three samples were found positive by PCR test while other detection methods were not that sensitive to detect the tuberculosis at that stage. Two patients were reported with history of tuberculosis but were effectively treated before imprisonment. On age basis...
Ghufran ul Haq1*, Muhammad Arif2, Asad Ali2 and Mian Inayatullah3
...virus were determined by inoculation of tomato plants of susceptible cultivar, Roma-VF, by whiteflies, determining the minimum time required for acquisition and inoculation of the virus, latency period and the persistence capacity. The highest incidence of 9.47 percent of TYLCV was recorded in Mohmand Agency, followed by Malakand Agency with 8.2 percent, Shabqadar with 7.7 percent, Charsadda with 4.9 and Peshawar with 5.8 pe...
Zain ul Abidin1, Aisha Khatoon2, Abdul Whab Manzoor1,*, Nida Arooj1, Sajjad Ali1 and Muhammad Numan1
... between 11-15 days post inoculation while all the mice were found dead between 15-18 days post inoculation in mouse inoculation test (MIT). Reverse transcriptase-polymerase chain reaction (RT-PCR) performed for both samples gave a product of 443-bp amplifying the highly conserved “N”-region gene of virus confirming the rabies infection in both cases.
... 
Ihsan Ali1#, Asim Aslam1*, Habib-ur-Rehman2, Beenish Zahid3, Ishtiaq Ahmed1
Effect of fish oil on growth performance and immune response in experimentally infected broiler chicken with avian influenza virus H9N2
...h
CO before virus inoculation, furthermore a significant difference was observed in
FCR after challenged, with highest value of group A, followed by group D, B and C.
The Geometric mean titer (GMT) for H9N2 and Newcastle Disease Virus (NDV)
were recorded weekly up to 42 day of age by haemagglutination inhibition test. The
mean GMT for H9N2 of group A, B, C, and D after challenge were 76.0, 273.7, 0.5
and 0.35, respecti...
CO before virus inoculation, furthermore a significant difference was observed in
FCR after challenged, with highest value of group A, followed by group D, B and C.
The Geometric mean titer (GMT) for H9N2 and Newcastle Disease Virus (NDV)
were recorded weekly up to 42 day of age by haemagglutination inhibition test. The
mean GMT for H9N2 of group A, B, C, and D after challenge were 76.0, 273.7, 0.5
and 0.35, respecti...
Ali Zohaib1*, Saima Yousaf1,2, Shakeel Ahmad Anjum1, Tahira Tabassum1, Tasawer Abbas3, Wardah Muzaffar1 and Wasiq Ikram2
...d can be applied as seed inoculation to enhance nutrient use efficiency along with reduced nutrient losses. A field experiment was conducted to evaluate the effect of seed inoculation viz. control (no treatment), Rhizobium japonicum (nitrogen fixing bacteria) and Pseudomonas fluorescens (phosphorus solubilizing bacteria) on growth, allometric traits and dry matter yield of different soybean genotypes viz. EBR4V4, Freedom and...
Nighat Seema1*, Muhammad Hamayun1, Husan Ara1 and Raham Sheer Khan2
... study, endophytic fungi inoculation significantly affected the soil properties such as soil texture, organic matters, EC and pH with or without 8% PEG induced drought stress. Upon inoculation of different endophytic fungi with or without drought stress, the trend in pH values of soil remain variable (7.61~7.96) compared with the pH value before experiment. The 8% PEG induced drought stress elevated the EC value, however, th...
Effect of Seed Inoculation with Plant Growth Promoting Rhizobacteria on Yield and Quality of Soybean
Saima Yousaf1,2, Ali Zohaib2*, Shakeel Ahmad Anjum2, Tahira Tabassum2, Tasawer Abbas3, Sohail Irshad4, Usman Javed5 and Naila Farooq6
...rmine the effect of seed inoculation with PGPRs on yield and quality of different genotypes of soybean. Seed inoculation treatments [control (no treatment), nitrogen fixing bacteria (Rhizobium japonicum) and phosphorus solubilizer (Pseudomonas fluorescens)] were applied to three different cultivars of soybean (EBR4V4, Freedom and Swat-84). The results showed that seed inoculation with R. j...
Ummad-ud-Din Umar1, Syed Burhan-ud-Din2, Muhammad Fahad Khan1, Ateeq ur Rehman1, Syed Atif Hasan Naqvi1*, Muhammad Asif Zulfiqar3, Azhar Ali Khan3 and Naila Ilyas1
...eated plants followed by inoculation with MYMV. Increase in resistance by the application of SA and BTH, antioxidant enzymatic activities of SOD, POD, CAT were also increased for second week. This revealed that SA and BTH can be used as a potential source for the activation of resistance for better management of MYMV by improving protection level by the induction of systemic acquired resistance. ...
Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3*
...ols as well as optimized inoculation techniques are required. The pathogen inoculation method should be less cumbersome, simple, and rapid. Here, we compared the effectiveness of six different inoculation methods, i.e., dipping, paste, wound+paste, soil infestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was significantly in...
Attaur Rahman1, Sadeeq ur Rahman1,*, Ashfaq Ahmad2, Abdul Sajid1, Muhammad Asif Hussain1, Mian Muhammad Salman1, Niamat Ullah Khan1, Kamran Khan3, Fazli Rabbani1, Muhammad Akbar Shahid4, Irshad Ahmad5 and Shakoor Ahmad6
...triches on days 0-7 post-inoculation. Interestingly, morbidity was observed 100%, while maximum mortality of 100% in broiler and 33% in ostriches was observed on day 7 and 6 post-inoculation, respectively. Interestingly, gross lesions were found similar comprising hydropericardium, hepatomegaly, hepatitis, nephritis and spleenomegaly. Altogether, albeit, FAdV-4 caused similar symptoms and lesions in ostriche, however, mortal...
Fraza Ijaz1*, Umair Riaz2, Shazia Iqbal3, Qamar uz Zaman4, Muhammad Furqan Ijaz5, Hina Javed1, Muhammad Amjad Qureshi1, Zuhra Mazhar3, Ahmad Hassan Khan6, Hassan Mehmood7 and Ijaz Ahmad8
...ht were recorded with Co-inoculation + Tryptamine @ 10-5 M (T8). Nutritious Parameters crude protein (30.23%), neutral detergent fiber (33.45%) and acid detergent fiber (26.56%) gave significant results as compared to control (T1). Results indicated that the combined application of Rhizobium species and Tryptamine performed better by improving growth and yield and quality parameters. It is concluded that precursor-inoculum combination is an effective approach ...
Imdad Ali Mahmood1, Muhammad Imran2, Muhammad Sarwar1, Matiullah Khan1, Muhammad Aqeel Sarwar2, Shoaib Ahmed1 and Shahid Riaz Malik2
...n plots with and without inoculation and Zn application in sub-plots) with three replications was followed. Agronomic data i.e., plant height, pods per peduncle, seeds per pod, 100-seed weight, total biomass and grain yield were recorded. Plant samples were collected at maturity to determine Zn concentration in plant tissues and grains. A significant increase in pods per peduncle, seeds per pod, grain yield and total biomass of lentil was observed with Zn appl...
Syeda Shazia Bokhari*, Ghashia Qayyum, Uzma Rafi and Aisha Waheed Qurashi
...howed positive impact of inoculation on vermicomposting in the presence of L. terrestris. This is beneficial for improving soil fertility and aggregation.
...
Muhammad Shafique, Nosheen Noor Elahi*, Muhammad Rashid, Amjad Farooq and Kausar Hussain Shah
Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3
...0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (30 °C), incubation period (at 7 days) and initial pH 5.0. Under these culture conditions, the maximum level of xylanase activity (9.29 Uml-1), CMCase (7.37 Uml-1), laccase (13.57 Uml-1) and effluent decolorization (69.68 %) were observed on 7th day during shake flask studies....
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *
...sults suggested that the inoculation with Bacillus genus bacteria improved the microbial proliferation and shortened the time that the substrate reached to the fermentation temperature, resulting in the retention of N, P, K and the reduction of the NH3, H2S from the fermentation litter.
... 
