Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
...phatase (ALP), lactate dehydrogenase (LDH), total protein, total bilirubin, hepatic glutathione (GSH), nitric oxide (NO), superoxide dismutase (SOD) and lipid peroxides (LP) levels were estimated. Moreover these biochemical parameters were confirmed by histopathological examination using hemotoxylin and eosin (H&E) and Mason trichrome stains (MTC). Immunohistochemical investigations for the expression of the proapoptotic protein (Bax) and the expression of...
Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*
...sperm-specific lactate dehydrogenase (LDH-C4) gene Ldh-c is expressed in plateau pika skeletal muscles. In order to shed light on the effect of LDH-C4 on the anaerobic glycolysis in plateau pika skeletal muscle, 20 pikas were randomly divided into two groups the inhibitor group (experimental) and the control group, reach of 10 pikas. The pikas of experimental group were injected with 1 mL of 1 mol/L N-isopropyl oxamate, a sp...
Maoying Zhu, Yuntao Ji, Xiaoyu Wang, Junying Pu and Changqing Qu*
...ectants with and without hydrogen gas (H) (purified H2 was dissolved into normal cryopreservation solution for 2 hrs under 0.6 MPa were tested including the following groups: control (CK), 100µm GSH, 10µm fasudil hydrochloride (FH) and a combination of the two (GSH + FH). A solution of 10% (v/v) Me2SO + 20% FBS was used as the standard cryopreservation solution. After 2 months, MTT assay showed significant differences in the p...
Asma Akhtar, Abdul Rehman
...n. Glucose 6 phosphate dehydrogenase (G6PDH) activity was increased to 279% (1f) and 150% (2b) when ompared to the respective control indicating the ability to tolerate tellurite tress. Differential pattern of bacterial proteins in tellurite stress was obtained by SDS-PAGE. Both bacterial strains can be utilized to convert toxic tellurite into its elemental form from the contaminated sites....
Amtul Jamil Sami1,*, Sehrish Bilal1, Madeeha Khalid1, Muhammad Tahir Nazir1 and A.R. Shakoori2
...in molecule to form four hydrogen bonds. Whereas in A. mellifera hydrogen bonds are formed at two positions by SER 171 and TYR104 with the saponin molecule indicating weak interaction as compared to T. castaneum Saponins derived from A. indica work as biosafe pesticides as it has no considerable effect on CNS enzymes of A. mellifera (a major plant pollinator and friendly insect) as compared to ...
Mahmoud M. Al-Azzazy1,2 and Saleh S. Alhewairini1,*
...TR50 is a formulation of hydrogen peroxide, stabilized by the addition of a small quantity of silver and is extensively used as a disinfectant. It was found to be very effective in killing A. lycopersici with no significant effect on N. cucumeris. Under greenhouse conditions, the mortality percentages were 81.17, 84.11, 92.74 and 95.10% for A. lycopersici and 15.86, 23.45, 33.33 and 58.19% for N. cucumeris after one week of exposure...
Rida Zainab1, Sana Elahi1, Afshan Kaleem2,*, Daniel C. Hoessli3, Mehwish Iqtedar2, Roheena Abdullah2, Faiza Saleem2, Shanza Khan1, Anusha Ijaz1, Shagufta Naz2 and Abdul R. Shakoori4,5,*
Fan Shuli1*, Ameer Hussain Jarwar1,3, Xiaoyan Wang2, Long Wang1 and Qifeng Ma1
...ing oil alternatively of hydrogenating it is also known as ghee in (solid form), devising cotton production more vulnerable to abiotic and biotic menace. ...
Jian Wang1,*, Zhi Liu2, Sheng Xu2, Mei-Fen Hu2, Xiao-Rui Liu1 and Wen-Jie Cai1
...eraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference, the ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and expression of CXCL8 mRNA in neutrophils of the chronic renal failure were (128.892±17.101) pg/mL and (1.332±0.138) lgcDNA/lgGAPDH. There was no significant difference compared with the normal controls (t=2.8468, P=0.062; t=1.953, P=0.0559). Fur...
Tafail Akbar Mughal1, Muhammad Zubair Saleem2, Shaukat Ali3,*, Khawaja Khurshid Anwar1, Muhammad Majid Bashir1, Muhammad Babar4 and Muhammad Adeeb Khan1
...rase) and LDH (lactate dehydrogenase) and biochemical components like glucose, urea, lipids, cholesterol and protein contents both in the liver and blood while DNA and RNA contents only in liver. The administration of CCl4 resulted in increase in plasma ALAT and decrease in LDH. Vitamin E pre-treatment abolished CCl4-induced changes in the activities of these enzymes. Blood glucose content was increased while cholesterol content was decre...
Muhammad Usman Ahmadand Ikram-ul-Haq*
...trains (Anaerobaculum hydrogeniformans OS1, Akkermansia muciniphila, Bilophila wadsworthia, and Roseburia intestinalis) were selected as experimental models for this purpose. Significant improvement in cell density of Anaerobaculum hydrogeniformans culture with maximum OD600nm of 0.80±0.0017 reached after 4 days of incubation when oxyrase used as reducing agent, and maximu...
Saleh S. Alhewairini1,* and Mahmoud M. Al-Azzazy1,2
...TR50 is a formulation of hydrogen peroxide, stabilized by the addition of a small quantity of silver and is extensively used as a disinfectant. Both tick species were susceptible to certain concentrations of Huwa-San TR50: 4,000, 8,000, 10,000, 12,000 and 14,000 ppm. A marked reduction in the movement of both tick species was observed 24h of applying Huwa-San TR50, at 10,000 ppm and above. This study recorded an increase in percentage mortality with increasing...
Uzma Jabeen1, Irfan Khan2, Nadia Naeem2, Asmat Salim2,* and Waseem Ahmed1
...els of NAD(P)H quinone dehydrogenase 1 (Nqo1), glutathione peroxidase (Gpx1) and isocitrate dehydrogenase-1 (Idh1) were analyzed by RT-PCR. We observedvariable pattern of gene expression was observed. Nqo1 and Idh1 genes were upregulated significantly in heart but not in liver tissue. Gpx1 seems to be unaffected both in heart and liver tissues. It is concluded from this study that toxicity due to doxorubicin is variable in t...
Xiaopeng Tang1,2, Rejun Fang3,4,*, Guochang Pan1,2 and Kangning Xiong1,2,*
... to detect the lactate dehydrogenase (LDH) activity of isolated small intestine at time on 0 min, 30 min, 60 min, 90 min and 120 min, to provide a appropriate operation time for Ussing chamber test. Then, a total of 20 Loman laying hens with similar weight (1.25±0.13 kg) were divided into 1 of 5 treatments: i) control group (0 ng/mL EGF), ii) 50 ng/mL EGF group, iii) 100 ng/mL EGF group, iv) 150 ng/mL EGF group, and v) 200 ng/mL EGF group for using...
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *
...e content of ammonia and hydrogen sulfide were increased at the first weekend, reaching up to 10ppm and 3.5 ppm respectively. Three weeks later, the content of the ammonia (NH3) and hydrogen sulfide (H2S) were decreased to 0.3 ppm and 0.6 ppm respectively. Total N, P and K were increased gradually with time, whereas the content of ammonium nitrogen was decreased. SAS software analysis showed that there ...
Ping Jiang1 , Zhihui Zhao1, 2, Xiaohui Li2, Mengyan Wang2, Lixin Xia2, Yang Cao3, Runjun Yang2 and Xibi Fang2*
...in 4 (FABP4), acyl-CoA dehydrogenase short/branched chain (ACADSB), ELOVL fatty acid elongase 3 (ELOVL3) and solute carrier family 27 member 1 (SLC27A1), were altered upon ABCA1 down-regulation using RNA interference (RNAi). Overall, ABCA1 expression influenced triglyceride metabolism in BMECs by regulating the expression of related genes in the lipid metabolism pathway.
...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*
...ells was investigated. Dehydrogenase activity was detected by a lactate dehydrogenase assay kit; superoxide dismutase (SOD) activity and malonaldehyde (MDA) concentration were detected by a total superoxide dismutase test kit and a malondialdehyde test kit. Genomic DNA and RNA were extracted from cells; Methylation was inhibited by treating HL7702 cells with 5-aza-2’-deoxycytidine (AZA), and the level was analyzed via ...
Sibgha Noreen*, Sumrina Faiz, Muhammad Salim Akhter, Kausar Hussain Shah
...f proline. The amount of hydrogen peroxide and Malondialdehyde were elevated by 170.3% and 68.7%, respectively while enhanced by 54% and 47.4% by spray of proline under salinity. Significant reduction in K+ uptake with concurrent increase in Na+ content was observed under NaCl salt stress, while the foliar application of proline increased the amount of K+ at vegetative growth under stress and non-stress conditions. The biological yield, physiological, and oxid...
Awais Ahmed Khan1*, Zafar Iqbal1 and Muhammad Atiq2
...0g of sample). Amount of hydrogen peroxide (H2O2), the signaling molecule was also significantly increased (30.33 mmol/ mg FW) and catalase (CAT) was higher (91 U/min g protein) in fruit treated with ascorbic acid as compared to control (22.67 U/min g protein) treatment. These results indicated that application of organic elicitors could increase the disease resistance by H2O2 elevation and by induction of antioxidant enzymes. This study was planned to evaluat...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...itrogen (BUN), lactate dehydrogenase (LDH) activity, cytokines (TNF-α, IL-1β, IL-6) concentrations, urinary volume, creatinine, and both serum and urinary electrolyte levels were measured. In addition, the apoptosis rate of white blood cells was analysed from plasma samples. Tissue samples from the brain, heart, aorta and kidneys were used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (...
Bin Huo1 and Xiaoyun Shen1,2,3*
...plementation of disodium hydrogen phosphate in affected Tibetan gazelles successfully treated the illness. This study demonstrated that the ailment of the Tibetan gazelle was mainly caused by the phosphorus deficiency in forage.
...
Yan Xu1, Jia Zhou1, Guangfu Lv2, Yuexin Liu1, Xintong Zhao1, Xin Li1, Doudan Ye2, Xiaobo Qu1,* and Xiaowei Huang1*
...DOX+PAP group. Lactate dehydrogenase (LDH) leakage and creatine kinase MB (CK-MB) level were used to detect cell viability, flow cytometry was used to detect cell cycle and apoptosis, and immunofluorescence was used to detect Bax and Bcl-2 expression. The protein levels of TGF-β/ Smad / ERK signaling pathway were detected by Western blot. We found that PAP significantly increased cell viability after DOX-induced injury, reduced LDH, CK-MB levels, up-regul...
Murtala Muhammad, Ting Zhang, Siyu Gong, Jing Bai, Jiansong Ju, Baohua Zhao* and Dong Liu*
...ood killing, exposure to hydrogen peroxide, and biofilm assays. This study represents the first Report of S. iniae infection of Chinese sturgeon (Acipenser sinensis).
...
Neelam Pery*, Nayab Batool Rizvi, Muhammad Imtiaz Shafiq
...aracteristic position of hydrogen bond donors and acceptors, it can accommodate the conformational changes of the binding cavity while maintaining interactions with the ATP binding residues. The designed inhibitors show some selectivity for JAK 1 and 3 over JAK2 offering a better safety profile against the harmful effects of JAK2 inhibition.
Muhammad Mazhar Iqbal1*, Ijaz Ahmad2, Shahzad Maqsood Ahmed Basra3, Abu Baker Ijaz4, Zahid Hassan Tarar5, Muhammad Ansar6, Umer Iqbal7, Tayyaba Naz4, Bilquees Fatima8, Muhammad Akram9, Allah Wasaya10, Asif Iqbal11, Shakeel Ahmed Anwar12, Khalid Saif Ullah Khan13, Azeem Khalid14, Asif Aziz15 and Rashid Mehmood16
...and their size. Spray of hydrogen peroxide (H2O2), salicylic acid (SA) and ascorbic acid (AsA) may induce heat stress tolerance. Spray of SA, AsA, and H2O2 increased chlorophyll, relative water and nutrient contents, membrane stability index (MSI) and antioxidants activities in heat stress. Moreover, foliar application of chemicals during normal and late planting improved the grain yield by increasing both the grain number and size. Foliar spray of SA, AsA and...
Haiyan Yang1, Hongxia Liu1, Wenlong Wu1*, Weilin Li2 and Lianfei Lyu1
... protein, soluble sugar, hydrogen peroxide (H2O2), malondialdehyde (MDA), ascorbate (AsA) and reduced glutathione (GSH), activities of superoxide dismutase (SOD) and peroxidase (POD) in leaves of ‘Hull Thornless’ were investigated. In the treatment group, water stress significantly increased EL, and the accumulation of photosynthetic pigments, protein, soluble sugar, H2O2 and MDA. After re-watering, all of those parameters showed decreases of diffe...
Taqi Raza1*, Sergio de Los Santos Villalobos2, Muhammad Shehzad3, Shakeel Imran4 and Derly José Henriques da Silva5
PGP Characterization of Rhizobacteria Associated with Apple Gourd (Praecitrullus fistulosus L.)
...d 14% strains showed the hydrogen cyanide (HCN) production abilities by slightly changing the color on media. While, in the case of enzyme production test, almost 90%, of isolated strains confirmed the amylase and pectinase production activities and 36% confirms the protease activities. Similarly, 82% and 45% of isolates confirmed the solubilization of zinc (Zn) and phosphorus (P) respectively. Almost, the entire isolated strains were given positive results fo...
Muhammad Rashid, Mahmood Ahmad Sajid, Nosheen Noor Elahi, Sibgha Noreen and Kausar Hussain Shah*
...ontents were. Similarly, hydrogen peroxide (H2O2) and malondialdehyde (MDA) were improved in all three wheat varieties but this increase was relatively lower in variety F23. The superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities were improved in all wheat varieties in a water shortage. The variety F23 exhibited higher antioxidants levels which are positively associated with its potential of drought stress tolerance...
Li Ma1 *, Xu Han1, Bo Yang2 and Chunhua Zhou3
...de) assay was applied in hydrogen peroxide-treated human umbilical vein endothelial cells (HUVECs). The hydrogen peroxide-induced apoptosis was gauged using flow cytometry whereas western blotting was applied to detect the expression of key cellular markers in oxidation pathways. The data indicate that a total of 50 µmol/l concentration of kaempferol can profoundly protect the oxidative damage in HUVECs. However, compa...
Muhammad Aftab1*, Aneela Riaz2, Ghulam Sarwar3, Muhammad Arif1, Qudsia Nazir1, Ifra Saleem1, Sarfraz Hussain1, Abid Ali4, Abid Niaz1, Fakhar Mujeeb4, Khalid Mahmood5 and Sarfraz Nawaz6
...hydroxyl radicals (OH-), hydrogen peroxide (H2O2) and superoxide (O2-). However, on their side, efficient antioxidant defense system developed in plants, which having lower molecular weight of antioxidants. It also reduces (ROS) enzymatic activities including catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Under combined stress conditions agronomic, bio-chemical, quality and over all yield reduces, which is an alarming situation for agricultur...
Luis Daniel Jiménez Martínez1, Vicente Morales Garcia2, Carlos Alfonso Frias Quintana3, Alejandra del Carmen Castillo Collado1, Gloria Gertrudys Asencio Alcudia4, Carina Shianya Alvarez Villagomez4, Emyr Saul Peña Marín4,5, Bartolo Concha Frias1 and
Carlos Alfonso Alvarez-Gonzalez4
...eraldehyde 3-phosphate dehydrogenase (gapdh) in the different tissues of the intestine, muscle, gill, stomach, brain and liver in adult males of A. tropicus from reared in captivity through three BestKeeper, geNorm and NormFinder algorithms. Based on our results we can conclude that in the three BestKeeper, NormFinder and geNorm algorithms, the most stable genes are ef1, followed by 18s rrna and actb where the gene stability ...
Lin Ye, Jie-Lan Jiang*, Jia Xu, Rui-Ting Liu, Yin-Qiu Tian, Qing-Qing Zang, Jia-Yi Cao and Ming-Guang Mao*
...nel 5 (CLCN5) transports hydrogen ions into the nucleolus and removes chloride ions, which helps the cell to maintain its pH value and osmotic pressure. To reveal the role of CLCN5 under low-salinity stress, a cDNA sequence of CLCN5 (TrCLCN5) was cloned from Takifugu rubripes using the reverse transcription PCR (RT-PCR) technique. The results showed that TrCLCN5 cDNA contained a complete open reading frame of 2505 bp continuously encoding 834 amino acid residu...
A.M. Abdul Azeem, Ashraf M. Mounir and Amr N. El-Shahat*
...e activity of xanthine dehydrogenase, superoxide dismutase and catalase) in the group of alloxan (150 mg/kg B.WT)-administrated rats as compared to control group. Treatment of diabetic rats with GPSDP (1 g/kg B.Wt/day/ 8 weeks) resulted in significant reduction of glucose level and lipid-peroxidation with amelioration of hormonal disturbance and enhancement of testicular antioxidant status with relative to alloxan-induced diabetic group. Therefore, this study ...
Tran Thi Bich Ngoc1,2, Ninh Thi Huyen1,2, Nguyen Cong Oanh2, Pham Kim Dang2*
...ase in ammonia (NH3) and hydrogen sulfide (H2S) concentrations were observed in diets with PROB or ORAC or PROR, with lower values for PROR diet. In conclusion, in grower-finisher pigs, dietary supplementation of either PROB or ORAC as a single product or their combination had improvements in performance parameters, nutrient digestibility, and fecal NH3 and H2S emissions compared with CONT diet. There were no differences in performance parameters between the u...
Mahreen Alam*, Muhammad Ashfaq, Sarfraz Hassan and Asghar Ali
A.H. Alqhtani1, A.S. Alharthi1, N.J. Siddiqi2, S. Zargar2 and A.M. Abudabos1*
... enzymes viz., lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) increased after feeding on growth promoters. However, total serum protein, gamma glutamyl transferase (GGT) and alkaline phosphatase (ALP) showed no significant differences between the groups. Thus, it can be concluded that probiotic, prebiotic and symbiotic in feed may cause some degree of liver damage as indicated by the release of AST and ALT in t...
Doaa Sh. Mohamed1, Nema S. Shaban2 , Mai M. Labib3, Olfat Shehata4
... - MB (CK-MB), Lactate Dehydrogenase (LDH), and Troponin-1 induced by doxorubicin injection were neutralized by almond oil supplementation. Almond oil ameliorated all the histological alterations caused by doxorubicin. The administration of almond oil with doxorubicin modulated the doxorubicin-induced changes in serum and cardiac tissue in mice due to its anti-inflammatory and antioxidant properties.
Keywords | Doxorubicin, Medicinal plant, Transmembra...
Uzma Jabeen1, Asmat Salim2*, Irfan Khan2, Nadia Naeem3 and Rubina Mushtaq4
...Gpx1), NAD(P)H quinone dehydrogenase 1 (Nqo1), 24-dehydrocholesterol reductase (Dhcr24), dual oxidase 2 (Duox2), and isocitrate dehydrogenase-1 (Idh1) were analyzed in the kidney tissues by RT-PCR. Extent of injury was analyzed by the change in expression of kidney injury biomarkers, kidney injury molecule-1 (KIM-1) and osteopontin (OPN), while the effect of DOX was also analyzed in terms of changes in the anti-inflammatory ...
Roheela Yasmeen1* and Samia Chaudhry2
...sed levels of lactate de-hydrogenase and, bilateral patchy shadows or ground glass opacity in the lungs (opacities may be mild damage of one lobe or all five lobes). This virus has the potential to affect pregnant women, however, its prevalence was not noticed in new-borns. Time to recovery is generally two weeks. To reduce the spread of COVID-19, observing hygienic practices like frequent hand washing, social distancing and drinking warm water and chloroquine...
Ahmed M. Darwish1*, Hassan R. Darwish1, Dalia M. Mabrouk1, Mohamed A. Abdelhafez1, Ahmed M. Abdel-Salam2, Ibrahim E. Mohamed3, Ibrahim M. Farag1
... Egypt. The potential of hydrogen (pH), fat, protein, lactose, and solid not fat (SNF) were determined by biochemical methods in all milk samples. Different genotypes of β-LG and LEP genes were detected by single-strand conformation polymorphism (SSCP-PCR), and then validated by sequence analysis. The results of SSCP-PCR showed a monomorphic pattern for the β-LG gene and a polymorphic pattern for the LEP gene. The sequence analysis showed that the &b...
Mona M. Osman1, Kamelia M. Osman2, Manal Abu Elmakarem Mohamed1, Mahmoud E. Hashad2, Jeeser Alves Almeida3, Alaa Saad4, Octavio Luiz Franco5,6, Heba N. Deif 2*
Serdar Bektas* and Ozer Ayik
...nd glucose-6-phosphate dehydrogenase activity, blood samples were taken 1,3,7,14 and 21 days after the infection. Total red blood cell values, were significantly decreased in 7 and 21th day, a significant decrease in hemoglobin concentration noted only in the 21th day, significantly lower values in hematocrit, were observed at 7, 14 and 21th days, total leucocyte and thrombocyte counts, found significantly higher only in the 3th day, comparing with the control...
Hafiz Liaqat Ali1, Muhammad Tahir Hassan1*, Muftooh Ur Rehman Siddiqi2, Mehwish Arshad1 and Muhammad Mudassar Sharif3
... lean combustion. Adding hydrogen (H2) in NG can be a solution to lean NG combustion limits. Herein, the experimental research was accomplished to analyze the effect of hydrogen blending on the combustion, emission, and performance aspects of NG EQD210N-20 engines. The engine was run at six different working conditions which are characterized by engine load, engine speed, and hydrogen
Tao Ren1, Guiqiu Cao2, Xiao Han3, Feng Tan1, Qiaoli Chen4, Shicheng Yang5 and Haiyan Zhang6*
...protein (HDL), lactate dehydrogenase (LDH), creatine, troponin-T (TRT), cholesterol, C - reactive protein (CRP), and concentration of mitochondrial enzymes viz., Ca2+, Na+ and K+ ions were estimated in blood. Heart tissue was also isolated for caspase-3 activity. Our result showed that naringenin pretreatment significantly increased cardiac dysfunction via scavenged free radicals and a reduction of inflammatory reaction. Dose-dependent treatment of naringenin ...
Duong Thanh Hai1*, Tomas J. Acosta2, Tran Thi Minh Tu3
...-β hydroxysteroid dehydrogenase type 1 inhibitor) was exanimated. PF at a dose of 1000 nM (PF1000) decreased significantly the absolute conversion of Cs to Cr (p<0.05). Thus, the PF1000 was chosen for the further studies. Tissues of endometrium were collected at the follicular stage (days 19-21), and exposed to Cs (300 nM) or Cs (300 nM) combined with oxytocin (OT, 100 nM) in the presence or absence of PF1000 in 4 h. The PGE and PGF secretion in cultur...
Muhammad Ikram Ullah
...sis detected the loss of hydrogen bonding between Thr at 904 with Arg at 916 in mutant MAN2B1. It is concluded that a novel mutation is identified in MAN2B1 associated with a complex neurological disease. The results indicate huge heterogeneity in the Pakistani population due to consanguinity. The combination of next-generation sequencing has facilitated the identification of genes in complex disorders like alpha-mannosidosis....
Shad Mahfuz1,2†, Hong-Seok Mun1,3†, Muhammad Ammar Dilawar1, Keiven Mark B Ampode1,4, Veasna Chem1, Young-Hwa Kim5, Jong-Pil Moon6, Chul-Ju Yang1,7*
...e, humidity, ammonia and hydrogen sulfide concentration were measured on weekly basis. There were no significant differences on the final body weight, average daily body weight gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR), between the incubators. However, ADG was numerically higher (P > 0.05) and FCR was lower (P > 0.05) in solar heating incubator compared with the control incubator. The consumption of electricity with sol...
Qamar Javed Iqbal1*, Zainab Masood2, Asad Abbas3 and Sidra Munir4
Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*
Caiping Duan
...The contents of lactic dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD) and malondialdehyde (MDA) were detected by enzyme-linked immunosorbent assay. Results showed that compared with the control group, the low-dose propofol group had increased NO, decreased ET-1 and cTnT contents (P<0.05), and high-dose propofol group had further increased NO and further decreased ET- 1 and cTnT contents compared with low-dose propofol group (P<0.05...
Chenchen Liu1,2, Tong Wang1, Muhammad Khan3*, Xiaolin Cui2 and Yongming Li1,2*
...-regulation of lactate dehydrogenase A (LDHA) and glucose transporter-1 (GLUT1). Finally, ALT effectively suppressed growth and induced apoptosis in cisplatin resistant A2780/CR cells. Taken together, our findings suggest that ALT could be developed into a lead for effective treatment of ovarian cancer....
Fathin Faahimaah Abdul Hamid1, Mohd Farhan Hanif Reduan1*, Jasni Sabri1 , Faez Firdaus Jesse Abdullah2,Mohammed Naji Odhah1, Nur Athirah Binti Abdul Manaf1, Mohd Jefri Norsidin2 , Siti Nor Che Yahya1, Intan Noor Aina Kamaruzaman1, Nur Zul Izzati Mohd Rajdi1
...were increased lactate dehydrogenase levels post-infection with Mannheimia haemolytica (p<0.05). In conclusion, oxytetracycline and flunixin meglumine treatments does not have a great influence on the parameters evaluated in goats experimentally induced with Mannheimia haemolytica pneumonia. ...
Chang-Man Kim1, Sam-Churl Kim2 , Young-Ho Joo2, In-Hag Choi3*
...0 cm height and ammonia, hydrogen sulfide, methyl mercaptan, and dimethyl sulfide levels were measured twice a week at 10 cm height from the pig slurry pits. Over time, the addition of 0.05% and 0.1% AlCl3 decreased overall ammonia and hydrogen sulfide losses and average ammonia and hydrogen sulfide levels at 10 cm height and overall ammonia losses at 90 cm height from the pig slurry pits....
Xiaowei Huang, Yajie Wang, Jia Zhou, Junxiu Liu, Zhe Lin and Ruili Li*
...ldehyde (MDA), lactate dehydrogenase (LDH) and reduced glutathione peroxidase (GSH-Px) were detected by ELISA. The expression level of TLR4/NF-κB, and HandE staining was used to observe the renal tissue. In the results, compared with model group, the spleen index and whole blood viscosity was significantly decreased, while the thymus index, peripheral blood WBC, RBC, Hb, HCT, PLT levels, and NO, SOD, GSH-Px levels in serum were significantly increased. T...
Sami Ullah1, Choudary Ihtasham Ali1, Umar Ijaz Ahmed1*, Shoaib Nasir1 and Amjad Masood2
...forms; vegetable oil and hydrogenated oil which is commonly known as vanaspati ghee. Over time, due to changing eating preferences, health concerns, and quality issues, vegetable oil is rapidly replacing vanaspati ghee in the country. This study has investigated the impact of taste and health preferences and other factors on the choice of cooking medium. In this study, we have utilized a well-structured and pre-tested questionnaire for data collection from 250...
Physiological Effect of Glycyrrhizic Acid on Adrenal Insufficiency Induces by Glucocorticoid in Rats
Wafaa AL. Bnyean1,2 ,Zainab A.H. AL-Mousawi1*
...β -Hydroxysteroid Dehydrogenase enzyme (11β-HSD), and malondialdehyde were measured. The results revealed treatment with glycyrrhizic acid improved significantly (P<0.05) in serum cortisol, ACTH, CRH hormones, and (11β-HSD) enzyme concentration, while was a significant reduction in serum MDA level in the therapeutic and protective group compared with the positive control group. Our results concluded that glycyrrhizic acid improves the alterat...
Muhammad Abdul Mannan1,2, Sharmin Chowdhury2, Md Abul Hashem3,4 and Md. Hazzaz Bin Kabir1,5*
...chondrial nicotinamide dehydrogenase subunit 4 gene (nad4) to identify H. contortus, followed by direct sequencing. Out of the 400 samples tested, 186 (46.5%) were positive for H. contortus. The parasite was found in 51.3% of sheep and 43.6% of goats. Through the sequencing analysis, 45 nad4 genotypes and 10 ITS-2 genotypes were discovered. The prevalence of Haemonchus contortus isolated was not influenced by demographic factors such as age, gender, breed, and...
Tehreem Iqbal1, Roheela Yasmeen1* and Faheem Hafeez2
...hosphate test, Lactate dehydrogenase, and alanine amino transferase) and kidney (Blood Urea Nitrogen, Creatinine, and Uric acid) biomarkers were assessed. The highest levels of ALT and ALP were linked to liver injury and a higher weight in the liver were observed in BPA treated rats as compared to the control group. Similarly, an increase in blood parameters were recorded in BPA treated rats followed by post -treated rats and pre- treated group. It was conclud...
SADAF IFTIKHAR1, SAMAN SHAHID2*, MUHAMMAD UMAR HASSAN1 & UBAID ULLAH3
...count and LDH (lactate dehydrogenase) as primary biomarkers in diagnosing the COVID-19-associated coagulopathy (CAC) in severe and critical cases. 141 COVID-19 patients of severe and critical categories, were enrolled from an epicenter tertiary care hospital. The mean values of platelets were found normal, whereas, the mean values of LDH were found elevated in both severe and critical cases. There was no patient with low value of LDH in both groups. 87% patien...
Erni Damayanti1, Herry Sonjaya2*, Sudirman Baco2 and Hasbi Hasbi2
...ure the concentration of hydrogen peroxide (H2O2) in Bali cattle embryos produced in vitro. 542 oocytes were matured in TCM 199 medium, were fertilized and were cultured using CR1aa medium for 48 h. After 48 h of culture, the embryo developed into 2, 4, 8 and 16 cells. Furthermore, it was grouped based on the stage of development, each of which was re-cultured for 48 h. After that, its development was re-evaluated and H2O2 measurements were taken. The 2-cell e...
Irfan Safdar Durrani*, Noreen Asim and Ammar Sohail
...ng analysis revealed the hydrogen bonding interaction between cis-acting elements and their corresponding Transcription factors....
Ying Chen1, Chao-Zheng Li2, Zhun Yu3, Jia Zhou4, Yan Xu4, Zhe Lin4, He Lin4* and Xiao-Wei Huang4*
... activities of lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) isoenzymes in serum, and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and catalase (CAT) in myocardial tissue were detected by ELISA. Hematoxylin and eosin (HandE) staining was used to detect the histological changes of rat myocardium. Western blotting (WB) was used to detect the protein expression levels of nuclear factor erythroid 2-related factor ...
Shu-Zhi Qin, Wen-Pei Ling, Mei-Fang Yin, Chun-Yu Luo and Cheng-Guo Zhao*
...rase (AST) and lactate dehydrogenase (LDH) were measured by colorimetry; Apoptosis rate and reactive oxygen species (ROS) content were measured by flow cytometry; the expression of Bcl-2, Caspase-3, p38 MAPK, ERK1/2 and JNK proteins were determined by WB technique. The results showed that, compared with control group, LDH, AST content, ROS level, apoptosis rate, Caspase-3, p38 MAPK, ERK1/2, JNK protein expression in H/R group were significantly increased, whil...
Noor A. Namaa*, Hayder A.N. Al-Zamely
...v>Keywords | Succinate dehydrogenase, Ginseng xtract, Bicinchonic Acid Assay , Sperms, Nanoparticales, Rats
...
Shamsa Jabeen and Javed Iqbal Qazi*
...inase (CK) and lactate dehydrogenase (LDH) are myosin heavy chain associated molecules which do not have the capacity to cross muscle cell membrane under normal conditions. Their increase in serum concentration have been used as indicators for muscle damage. The aim of the current study was to determine the effect of Lactobacillus rhamnosus administration on CK and LDH levels in the serum of mice following extensor digitorium longus (EDL) muscle grafting. Bloo...
Feri Eko Hermanto1, Yuli Frita Nuningtyas1, Filoza Marwi1, Fajar Shodiq Permata2, Agus Susilo1, Muhammad Halim Natsir1*
...s, establishing multiple hydrogen bonds and demonstrating favorable binding affinities. Molecular dynamics simulations provide visual insights into the stability of these compounds during their interaction with IL-6. In summary, the potential mucosal immunomodulatory effects of Teak Leaves, Turmeric, and Ginger may be harnessed through IL-6 inhibition, thus fostering a healthier gut microstructure, optimizing nutrient absorption, and restoring physiological ba...
Sedef Selviler Sizer1, Semih Kurt1*, Burcu Onuk1, Gokmen Zafer Pekmezci2 and Murat Kabak1
...time. In addition, using hydrogen peroxide solution to kill remaining beetles in the cleaned osteological material and determine the whitening and degreasing efficiency of this solution was also aimed. In the study, approximately 10,000 Dermestes maculatus, along with the heads of six roe deer, three cows, and three cats were used. To observe the meat-cleaning performance, the initial and final weights of the heads were measured before they were placed in the ...
Shabnam Javed1, Amna Shoaib2 and Zaid Mehmood1
... fat, along with carbon, hydrogen, nitrogen and sulphur were analyzed in whole
plant of S. tomentosa. The results revealed the occurrence of considerable proportion of
carbohydrates (52%) and protein (23.80%). Moisture, fat and ash contents were found in
small amount i.e. 6.25%, 2.02% and 0.20%, respectively. Elemental analysis displayed the
highest content of carbon (44.92%) followed by ...
Mohammad Anis and Farooq Ahmed Dar
...d with gibberallic acid, hydrogen peroxide and control were tested for germination in the laboratory Highly significant differences were observed among the families, treatments and their interactions. The results suggest that in hormonal control of dormancy, gibbere Uric acid plays a primary role in regulation of germination.
Keywords: Cedrus deodara, germination, gibberellic acid.
...
Ashiq Ahmad and G. M. Khattak
...20 days. Soaking seed in hydrogen
peroxide for 15 minutes, 30minutes and one hour followed
by soaking in water for 24 hours gave, in 5 days 7, 8
and 11% germination, respectively. When soaking in
hydrogen peroxide was not followed by soaking in water,
germination was delayed by 5 days. Control and cold
water soaking for 24 hours gave only about 8%
germination in 20 days.
...
Bushra Irfan1, Muhammad Shahbaz1*, Asif Mukhtiar1, Muhammad Zubair Akram2, Muhammad Atif Bashir3, Sabina Asghar4, Abdul Ghaffar5 and Samreen Nazeer6*
...eroxidase) and oxidants (hydrogen peroxide and malondialdehyde), and lessened the uptake of essential minerals (potassium and calcium) irrespective of varietal differences. However, the performance of TH-6 was better than TS-5. In addition, the seed priming of thiourea enhanced the sesame photosynthetic pigments and efficiency, secondary metabolites production, antioxidant machinery, and nutrient uptake in both varieties which increased growth and development ...
Muhammad Ambar Islahuddin1, Yuli Retnani2*, Despal2
...iv>Keywords | Binder, Biohydrogenation, Dairy cattle, In vitro, Precision, Wafer supplements
...
Rawya Sh. Mohammed*, Baraa N. Al-Okaily
...synthesized using sodium hydrogen selenite and a hydroalcoholic extract derived from Curcuma longa. The in vivo study utilized thirty-two mature female rats divided into four experimental groups over a two-week treatment period as follows: Rats in the control group (C) received distilled water orally, Group G1 received intraperitoneal injections of doxorubicin (4.40 mg/kg B.W), Group G2 was administered CurSeNPs (10.47 μg/kg B.W), and Group G3 received both...
Rawya Shakir Mohammed*, Baraa Najim Al-Okaily
...NPs) by combining sodium hydrogen selenite with a hydroalcoholic extract obtained from Curcuma longa rhizomes in alleviating the disturbance of vascular endothelial growth factor (VEGF) mRNA expression, histomorphometric and histological alterations of ovaries in healthy rats that administered doxorubicin.Thirty-two fully grown female rats were randomly divided into four equal groups and were treated for two weeks as follows: The control group (C) of rats were...
Naglaa A. El-Taib1*, Asmaa T. Talayea2, Hanan R. Ghanayem3
...e effect of chlorine and hydrogen peroxide as common chemical sanitizers on inducing E. coli O157 into a VBNC state. The results showed that after 30 minutes of treatment with 200, 100, or 50 ppm chlorine, the numbers of culturable E. coli significance P<0.05 dropped (100%) from 6.93±0.04 log10 CFU/mL to less than 1 CFU/mL (0.0%). However, after 48 hours of resuscitation using sodium pyruvate as nourished media, the VBNC cells in case of used 200, 10...
Abrar ul Haq1, Shusheng Yin2, Amara Maryam1, Muhammad Khan1*, Hafiz Abdullah Shakir1, Muhammad Akhtar Ali3, Muhammad Irfan4, Muhammad Faisal Maqbool1 and Yongming Li2*
...A binding domain through hydrogen bonding and hydrophobic interactions with various amino acid residues of STAT3. Using immunoblotting, we found that lathyrol did not inhibit STAT3 phosphorylation and dimerization. Moreover, we showed that lathyrol induces apoptosis as evident from a remarkably increased expression of cleaved caspase-3 in all four cancer cell lines. Taken together, our findings suggest that lathyrol is a potent STAT3 DNA binding domain inhibit...
Kai Wang, Weifeng Xu, Pengyuan Dai and Chunmei Li*
...m malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide dismutase (T-SOD), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Meanwhile, the mRNA expression of HO-1 and NQO1 in liver tissue was also not significantly different. These results indicate that PNP may damage liver tissue through oxidative stress. Arg may improve growth performance and attenuate PNP-induced liver inflammation in healthy animals, but not by reducing oxidative ...
