Aradhana Chopra1, Ravi Shukla2 and Tarun Kumar Sharma2,3*
...specificity. The ease in synthesis and modification makes them an attractive candidate for a variety of applications. Since the last decade, aptamers have been regarded as an emerging player in biology. They have found applications not only in biosensors, but also in other areas such as diagnostics, biomarker discovery, drug delivery, bio-imaging and molecular therapy. In this review we have summarized recent advancements and applications of aptamers in biolog...
Amtul Jamil Sami1*, Madeeha Khalid1, Sara Iqbal1, Maira Afzal1 and A.R. Shakoori2
Faiqah Ramzan*1 and Muhammad Haris Ramzan2
... the prostate gland. Its synthesis is regulated by androgens. As, dose dependent degeneration of prostate gland has been described following intraperitoneal kisspeptin treatment. However, effects of kisspeptin administration on the levels of prostatic citric acid remain elusive till date. The present study, therefore, addresses the effects of 12 day administration of kisspeptin on prostatic citric acid levels in male mice. Kisspeptin-10 was administered intrap...
Shazia Ali, Hizb Ullah and Sarwat Jahan*
...absent beta globin chain synthesis, results in reduced Hemoglobin, decreased RBC production and anemia. Total 300 individuals were divided into 4 groups according to age and gender i.e ≤13 years females, >13 years females, ≤13 years and >13 years males. Height in centimeter, weight in kilogram was measured to calculate BMIKg/m2. Serum ferritin (ng/mL) and hemoglobin (gm/dl) alongwith hormonal assay of GH (ng/mL), T3 (ng...
Muhammad Qadir Ahmad¹*, Farheen Ashraf Raza¹, Abdul Qayyum¹, Waqas Malik¹, Rao Wali Muhammad2, Muhammad Asif Saleem1, Amir Hamza2, Ahmad Baksh Mahar3
...energy exchange and photosynthesis. The experiment was undertaken to study the association of leaf related traits i.e. leaf area (LA), leaf angle position (LP), number of lobes per leaf (NL), leaf chlorophyll contents (SV), relative water content (RWC), leaf hairiness (HL), leaf color (LC) and yield related traits i.e. boll weight (BW), number of seeds per boll (NS), number of locules per boll (NLB), seed index (SI) and seed volume (SEV). Twenty cotton genotyp...
Obaidullah1, Zafar Ali Shah1* and Sami Ullah2
...er-Lapachol nanoparticle synthesis was performed by treating 1 mM copper acetate tetrahydrate (Cu (CH3COO)2. 4H2O) with 1 mM solution of Lapachol at pH of 7.8 having temperature 40°C for 5 hours. Both newly formulated silver and copper nanoparticles were characterized using UV-visible, FT-IR spectroscopic techniques followed by SEM evaluation. The UV spectrum confirmed peaks at 415 nm and 560 nm for silver and copper nanoparticles, respectively. FTIR spect...
Mohammad Umar Farooq1*, Sarfaraz Ahmad2, Ghulam Nabi1, Ijaz Ali1 and Imtiaz Ahmad1
...stration during the photosynthesis process via plant biomass is the extent of this atmospheric gas. An experiment the data for above and below phytomass for grazed and un- grazed range land was collected and carbon pool was estimated by Wet Combustion and Dry Combustion method.Four transect lines were drawn in each experimental plot. Twenty four samples of each experimental plot were collected with the help of ADC one m 2 quadrate methods, weighed a...
Promy Virk
...y investigated the green synthesisofgold and silver (Au/Ag) nanocomposite using aqueous Trigonella foenum-graecum (fenugreek)seed extract and to evaluate the anti-diabetic activity of the prepared nanopcomposite. Antidiabetic potential of orally administered bulk aqueous extract of Trigonella (TBE) and Au/Ag nanocomposite with Trigonella seed extract (TN) on streptozotocin (70 mg/kg) induced diabetic rats was assessed for a period o...
Mohammad Umar Farooq* , Sarfraz Ahmad ** , Mohammad Islam and Imtiaz Ahmad Qamar ***
ASSESSING THE STATUS OF CARBON POOL IN TWO RANGELAND GRASSES ( ) IN POTHWAR, REGION, PAKISTAN
...stration during the photosynthesis process via plant biomass is the extent of this atmospheric gas. Data for above and below phytomass for two rangeland grasses was collected and carbon pool was estimated by Wet Combustion and Dry Combustion method. Four transect lines were drawn in each experimental plot. Twenty four samples of each experimental plot were collected with the help of ADC one m quadrat method, weighed and then oven dried at 60 C to find out the ...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
... of key genes related to synthesis, metabolism, and growth of adipose tissue during development. This will allow to determine the functional relevance of the genes and pathways uncovered.
...
Ayesha Noreen1, Dilara A. Bukhari2 and Abdul Rehman1,*
Muhammad Zahid1, Naeem Iqbal1, Sohaib Muhammad2*, Summiya Faisal3, Wajid Mahboob3, Makhdoom Hussain4 and Zaheer ud din Khan2
..., transpiration and photosynthesis rates were raised by glucose treatments under irrigated and non-irrigated conditions. Hence, drought tolerance was slightly induced by exogenous glucose doses in terms of improved growth, yield and physiological traits. ...
Shafaq Fatima*, Sumrin Sahar and Khalid Lone
... steroids suppressed the synthesis of cortisol presumably interfering with pituitary-inter-renal axis.
...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...ch can regulate collagen synthesis and deposition. To investigate collagen metabolism in keratinocytes and fibroblasts co-culture under pressure, and study the role of IL-1α and MMP-3 in terms of regulating fibroblasts-keratinocytes collagen metabolism with pressure.Chitosan-gelatin scaffolds were fabricated by vacuum freeze drying. Keratinocytes and fibroblasts were seeding on scaffolds to form systems as: keratinocytes monoculture (KM), fibroblasts ...
Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
... decrease TLR4/9 protein synthesis and expression. So we concluded that Wumei Pill probably reduce the release of pro-inflammatory cytokines and the bacterial endotoxins by blocking the transmission of TLR4/9-NF-kB signaling pathway so that Wumei Pill has a significant therapeutic effect on IBS-D.
...
Umair Riaz1*, Muhammad Ali Kharal2, Ghulam Murtaza3, Qamar uz Zaman4, Sana Javaid4, Hina Ahmed Malik3, Humera Aziz3 and Zafar Abbas1
...tive mechanism is the biosynthesis of secondary metabolites at cellular level which included organic compounds that help plants to cope with stress conditions by reducing the intensity of stress through enhancing antioxidants activities, detoxifying toxic ions, regulating the uptake of nutrients and by mediating the transport and distribution of different hormones. Caffeic acid is actively involved in plant physiology and mechanisms of stress tolerance primari...
Imran Khan1*, Muhammad Iqbal1 and Malik Muhammad Hashim2
...hlorophyll content, photosynthesis, plant height (cm), number of leaves plant -1, leaf length (cm), leaf area (cm2), leaf weight plant-1(g), root weight plant-1 (g), root/top ratio, root length (cm), root diameter (cm), sucrose%, tss %, purity %, leaf yield (t ha-1), root yield (t ha-1), sugar yield (t ha-1) and harvesting index. The results indicated significant variation among the treatments for all parameters during both years of study. Among the treatments...
Hua-Lun Luo, Yi-Yu Zhang*, Yuan-Yu Qin and Lei Wu
...ntral regulator of lipid synthesis and glycolysis in animal liver. In this study, the relative quantitative real-time PCR analysis indicated that the duck ChREBP mRNA is widely expressed in all examined tissues. ChREBP mRNA level was the highest in abdominal fat and the lowest in gizzard. The g.247075G>A silent mutation in exon 10 was first identified by direct sequencing approach, and resulted in three genotypes of AA, GA and GG. Association analysis demon...
Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2
Tahira Moeen Khan* and Amat ul Mateen
...sfully for nanoparticles synthesis and CuO as significantly active antibacterial material. Antibacterial activity of these nanoparticles was observed by agar well diffusion method.
...
Songhao Zhang1, Yiwen Gong1, Jian Xu1, Mou Hu2, Peng Xu3 and Yanliang Jiang1,2,4,*
...ays including melanin biosynthesis, TGF-beta signaling, Wnt signaling and MAPK signaling pathways. We obtained candidate genes that may affect the morphological characteristics of purse red carp resulted from the long history of domestication, including TYRP1b, PMELa, KITa, BMP8a, ACVR2aa, TGFB1a, TGFB3, TGFBR2. Understanding the molecular mechanisms underlying the biological characteristics of purse red ...
Constance Obiageli Ejilibe1, Helen O. Nwamba2, Ifeanyi Chinedu Atama3,*, Chiamaka Lynda Ani2, Ifeanyi Oscar Aguzie3, Josephine C. Madu3 and Christopher Didigwu Nwani3
...and depletion of protein synthesis. Both pesticides should be applied with extreme caution to avoid toxicity to non-target organisms.
...
Rashid Iqbal1,2, Mathias Neumann Andersen1*, Muhammad Aown Sammar Raza2, Muhammad Adil Rashid1 and Salman Ahmad2
...al conductance, net photosynthesis and transpiration rate. Magnitude of osmotic adjustment (i.e. leaf water, osmotic and tugor potential) was lowest and highest under FI, DI and PRD, respectively. However, PRD had lower shoot biomass and grain yield per plant as compared to FI but higher than the DI. Despite improving the WUE, PRD tended to compromise the yield of wheat crop but PRD is recommended instead of DI. Results imply that PRD treatment can be an optio...
Faleye Temitope Oluwasegun Cephas1,2*, Adewumi Moses Olubusuyi1, Olayinka Olaitan Titilola3 and Adeniji Johnson Adekunle1,3
... to RNA extraction, cDNA synthesis, three (5l-UTR, VP1 and EV Species C [EV-C]) different PCR assays and sequencing of amplicons. EVs were further subjected to [‘]Illumina sequencing. All CCS were negative for EAVs and GARVs. Four CCS produced R-CPE in HEp-2 cell line, three of which were positive for the 5l-UTR assay. Of the three isolates, two and none were positive for the VP1 and EV-C assays, respectively. One of the two VP1 amplicons was successfull...
Abdul Majeed1*, Muhammad Anwar-ul-Haq2, Abid Niaz3, Abid Mahmood4, Naeem Ahmad1 and Hafiz Muhammad Walyat Ali Khan1
...owed higher rate of photosynthesis and potassium contents than V05003 and V07194. The salt-tolerant and salt-sensitive genotypes, V07096 and V05003, respectively were then grown in soil medium. Lower plant sodium contents in soil medium were also observed in V07096 variety under salt stress treatments than V05003. Salt tolerant variety also showed higher potassium contents and photosynthetic rate in salt stress soil medium. Thus plant Na+, K+
Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2
...miting enzyme for Cys biosynthesis from tobacco i.e. NtSAT4 was successfully cloned into three types of overexpression constructs i.e. pBinAR_NtSAT4 (targeted to cytosol), pBinAR-TKTP_NtSAT4 (targeted to plastids) and pBinAR-SHMT_NtSAT4 (targeted to mitochondria). For stable transformation of B. napus floral-dip and tissue culture based approaches were tested using different formulations of phytohormones for calli, shoots and roots induction in a variety of ge...
Using Smartphone Application to Estimate the Defoliation Caused by Insect Herbivory in Various Crops
Muhammad Irfan Ullah1*, Muhammad Arshad1, Sajjad Ali2, Asad Abdullah1, Samina Khalid3, Hafiz Muhammad Aatif4, Syed Muhammad Ali Zahid1, Muhammad Afzal1 and Jaima Molina-Ochoa5
...is critical for the photosynthesis process. Thus, quantification of foliar area needed for the photosynthetic process is impartment. The recent methods used to quantify the foliar damage are relatively expensive and are mainly dependent on complex and state of art devices and instruments. Instead, smartphone application may be a good source to measure the foliar damage. In this study, foliar damage of Spodoptera litura Fab. (Lepidoptera: Noctuidae) on sunflowe...
Hui-Ying Chen1,2*, Ping-Chuan Yin1,2, Ya-Nan Lu1,2, Hai-Yun Li1,2*and Yang Shan3
... secondary metabolite biosynthesis, carbon metabolism and antibiotic effects. The results revealed that the substance exhibited abundant biosynthesis and glycolysis/gluconeogenesis. Subsequently, the following top 10 hub genes were identified to the PPI network: Guanine nucleotide-binding protein subunit β, ribosomal 60S subunit protein (RPL) 8A, RPL9A, RPL6B, ribosomal 40S subunit protein (RPS) 9B, RPL31B, RPL27B, RPS1...
Anila Latif, Zaheer Abbas, Farhatullah and Ghulam Muhammad Ali*
...BE) gene involved in the synthesis of berberine was isolated from Berberis lyceum and over expressed in Arabidopsis thaliana. The integration of the BBE gene in transgenic lines was confirmed through PCR while the expression was confirmed by Reverse Transcriptase PCR. Whole plant bioassay confirmed 100% mortality in DBM within 72 hours. The present investigation concludes that the metabolic engineering of berberine in plants is an effective strategy to protect...
Wajiha Anum1*, Liaquat Ali1, Umair Riaz2*, Abid Ali1, Nadia Manzoor3, Laal Hussain Akhter4, Asad Ur Rahman5, Naeem Maan6 and Ijaz Ahmad7
..., energy provision, photosynthesis, and continuation of metabolic function in plants. However, it is not readily available to the crop because of its immobile nature. In order to ensure its efficient uptake by crop, an experiment was conducted to appraise the effect of various P placement methods and fertilizer rates on wheat. The fertilizer treatments were F1=150-00-60 NPK kilogram/hectare, F2=150-30-60 NPK kilogram/hectare, F3=150-60-60 NPK kilogram/hectare,...
Arshad Habib Malik*, Aftab Ahmed Memon** and Muhammad Rafiq Khan***
DYNAMIC MODELING AND CONTROLLER DESIGN FOR NUCLEAR POWER PLANT
... output sampling control synthesis for PHWR (Pressurized Heavy Water Reactor)-type nuclear power plant is presented in this paper. The nonlinear dynamic model of PHWR power reactor is developed based on reactor, logarithmic amplifier, moderator level control valve and reactivity system. The nonlinear model is characterized by 15 state variables and one input. This higher order nonlinear model is linearized at full power operating point. A s...
Guang-Hui Tan, Yi-Yu Zhang*, Yuan-Yu Qin, Lei Wu and Jie-Zhang Li
...ys a crucial role in the synthesis of bile acid, fatty acid and cholesterol metabolism in human and rodent. However, a little was known in the literature on the question of the effect of CYP7A1 gene on poultry lipid homeostasis. This study was performed to investigate the effect of the polymorphisms of CYP7A1 gene on lipid traits in Tianzhu Black Muscovy (Cairina moschata). We detected two novel silent mutations, CDS 216 A>G and CDS 681 T>A in exo...
Anum Feroz1, Abdul Rauf Shakoori2 and Farah Rauf Shakoori1*
...t insects upregulate the synthesis of esterases in general cholinesterase and carboxylesterase in response to exposure to insecticide to combat with the stressful conditions of exposure to toxic chemicals. The activity of the arylesterase, on the contrary is inhibited to variable extent after exposure to the insecticides.
...
Tehmina Aslam, Shagufta Saeed*, Muhammed Tayyab, Huma Mujahid, Ali Raza Awan, Sehrish Firyal and AbuSaeed Hashmi
...ing and molasses for the synthesis of polyhydroxybutyrate (PHB) by Azotobacter vinelandii. The optimization of different carbon sources and various physical parameters was also performed. Highest yield of PHB was obtained by fermentation of 4% (w/v) wheat bran after 48 h of incubation with 4% (v/v) inoculum volume at pH 7.0 and 37oC. Among different nitrogen sources tested, 0.2% peptone gave the better yield (285 mg/100 mL), while yeast extra...
Yan Zhou1,2, Hai Xia Han1,2, Qiu Xia Lei1,2, Jin Bo Gao1,2, Wei Liu1,2, Fu Wei Li1,2, Jie Liu1,2 and Ding Guo Cao1,2*
...duction in mediating the synthesis of yolk protein precursors. To better understand the effects of VLDLR on reproduction in chickens, the haplotypes and diplotypes based on three genetic mutations (NC_006127.2:g.8467G>A, NC_006127.2:g.12321G>A and NC_006127.2:g.13876A>G) were constructed, and the associations of diplotypes with reproduction traits were assessed, their effects on gene expression were evaluated also. As a result, three haplotypes H1 (G-...
Hong Hu1, 2, Li Qian1, Yuanlang Wang1, Chaodong Wu1, Xiaodong Zhang1, Yueyun Ding 1*, Li Wang1, Xudong Wu1, Wei Zhang1, Dengtao Li1, Jian Ding1, Min Yang1 and Zongjun Yin1*
...receptor interaction, biosynthesis of unsaturated fatty acids, fatty acid metabolism, regulation of lipolysis in adipocytes, glycerolipid metabolism, etc. The results of differentially expressed genes from sequence were highly reliable by qRT-PCR confirmation. The present work will help understanding of the different mechanisms involved in fat deposition between lean and obese pigs.
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