Reviewed by Elly Vintiadis (www.ellyvintiadis.com)
Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
...ile spermatozoa ml-1) in tris citric acid extender with melatonin at 0 (control), 0.1, 0.5, 1, and 1.5 mM. Diluted semen was cooled to 4°C for 2 h and equilibrated for 4 h at 4°C. Straws were then kept over liquid nitrogen vapours for 10 min and plunged in liquid nitrogen for storage. Thawing was performed after 24 h of storage, at 37°C for 30 seconds and post-thaw sperm quality parameters were assessed. Sperm progressive motility, plasma membrane ...
Muhammad Imran*, Munir Ahmad, Muhammad Naeem, Muhammad Farooq Nasir, Imran Bodlah, Muhammad Nasir and Umer Ayyaz Aslam Sheikh
...f bumblebee Bombus terrestris (L.) (Hymenoptera: Apidae) were investigated by using rearing boxes of wood, cardboard and polycarbonate materials on three developmental stages i.e., colony initiation, development and maturation stages under controlled laboratory conditions. Queen oviposition rate was 1.4-fold higher in polycarbonate box compared to wooden and cardboard materials. Early start of egg-laying (first oviposition period) and earlier emergence of firs...
Ali Raza Awan*, Sehrish Firyal, Muhammad Tayyab, Lala Rukh, M. Zia ul Haq, Shagufta Saeed and Muhammad Wasim
...meri, P. krameri parvirostris, P. krameri manillensis and P. krameri borealis). Identification of the sub-species is an intricate chore. This study aimed to genetically identify and classify the indigenous wild Rose-ringed Parakeet of Pakistan using Cytochrome b (Cytb) gene polymorphism. Mitochondrial DNA of 24 unrelated Pakistani wild Ring-rose Parakeets was isolated and utilized for amplification and DNA sequencing of Cytb gene. The Phylogenetic analysis of ...
Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3
...tion), Xanthomonas campestris (67.00 ± 0.46% inhibition) and Clavibacter michiganensis (72.00 ± 1.02% inhibition). The A. flavus was grown on two different media, i.e. Potato Dextrose Broth (PDB) and Glucose Nutrient Broth (GNB) media for active secondary metabolites. Ethyl acetate (EtOAc) extract of PDB medium gave significant results against selected phytopathogens compared to GNB medium and selected for further study. Crude (1 µg.mL-1 ) ...
Rashid Mahmood1*, Saima Asad2, Ghulam Sarwar1, Waqar Ahmad1, Ziyad Abdul Qadir1, Ammara Balouch1 and Muhammad Khalid Rafique1
...) from Brassica campestris was examined during February - March 2015 at National Agricultural Research Centre, Islamabad. Twenty five honeybee colonies were used which had similar number of worker bees, brood and stored food. In order to study the foraging activity of honeybees in the field condition, the number of pollen foragers returning to the hive entrance was recorded for 10 min duration on hourly basis between 0800 h to 1600 h for 3 days per week...
Sitansu Pan, Surojit Khalko and Amrita Das
Effect of some fungicides on seed mycoflora, germination, viability and their persistence in treated seeds
... mustard (Brassica campestris L.) and Drechslera oryzae in rice (Oryza sativa L.). Propineb 70WP and prochloraz 45EC considerably reduced the appearance of A. alternata and flusilazole 40EC reduced Fusarium sp.in mungbean seed than untreated control.Amarked reduction in population of A. brassicae by difenoconazole 25EC and flusilazole 40EC was observed in mustard seeds. Inhibitory effect of fungicides on seed germination of all tested seeds was observed with p...
M. D. Maji, N.K.Das, S. Chatterjee, A. Ghosh and A.K. Bajpai
Forecasting models of bacterial leaf spot disease of mulberry for Birbhum district of West Bengal
...sed by Xanthomonas campestris pv.mor was found to be one of the major foliar diseases in mulberry at farmers field in Birbhum district.BLS incidence appeared in May and continued up to November. The correlation coefficient between disease severity and meteorological parameters revealed that BLS disease severity showed significant positive correlation with max & min temperatures, min relative humidity, rainfall and number of rainy days. Step down multiple r...
Manidipa Chowdhury
Incidence of saw fly, Athalia lugens proxima Klug. as influenced by level of irrigation and fertilizers on mustard
...humka) of Brassica campestris var. yellow sarson. Highest saw fly population (0.26 larvae/plant) was recorded on the crop grown without irrigation and medium level of fertilizers (60:30:30 Kg NPK ha-1) while lowest population level (0.10 larvae/plant) was observed at highest level of irrigation (three) coupled with medium level of fertilizers (60:30:30 kg NPK ha-1) and two irrigations coupled with lowest level of fertilizers (40:20:20 Kg NPK ha-1).
Muhammad Ashfaq,1 Shamim Akhtar,2 Saleem Akhtar2,* and Mariyam Masood2
...Promuscidea unfasciativentris Girault (Hymenoptera: Aphelinidae: Eriaporinae). Traditionally the detection of hyperparasitoid is done by host-rearing, but that is time-consuming when working with field populations at larger scale. The current study used morphology and DNA sequences from mitochondrial (cytochrome oxidase I) and nuclear (internal transcribed spacer I) genes to identify the hyperparasitoid Promuscidea unfasciativen...
Jawad Anwar* and Zafar Iqbal
...teria (Xanthomonas campestris and Clavibacter michiganensis). Extracts that resulted in strong activity were fractionated into acetonitrile and ethyl acetate followed by testing against human pathogenic bacterial strains (Eschericia coli, Pseudomonas aeruginosa and Staphylococcus aureus) using 96 well microtitre plate method. The Ethyl acetate fraction showed maximum zone of inhibition (24.25±1.06 mm) against X. campestris
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...uffalo bulls, diluted in tris citrate egg yolk based extender and cryopreserved using standard protocol. The semen straws were thawed at 37oC and stained using different protocols as followed: Protocol 1 (washing effect); washing either with normal saline (NS) or phosphate buffered saline (PBS), Protocol II (heating effect during incubation in tampon solution); smears were immersed in tampon either at room temperature or at 60oC, Protocol...
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
Tariq Mahmood*1, Syed Wasif Ahmed Shah1, Muhammad Rais1, Hira Fatima1, Faraz Akrim1 and Muhammad Sajid Nadeem2
...mon myna Acredotheris tristis, house crow Corvus splendens, Indian robin Saxicoloides fulicatus and black drongo Dicrurus macrocerus preferred Kikar Acacia nilotica and Eucalyptus camaldulensis for nesting purpose while black kite Milvus migrans utilized semal tree Bombax ceiba. The weaver bird Ploceus philippinus also utilized only Acacia nilotica for nesting. The common crow, common myna, ...
Shahid Mehmood1,*, Bushra Nisar Khan1, Hassan Raza1, Rida Ahmad1, Azhar Muhammad3, Zulfiqar Ali2, Farhan Abid1, Fehmeeda Bibi4 and Syed Maviz Ahmed5
...on myna, Acridotheres tristis (n=327), black-crowned night heron, Nycticorax nycticorax (n=190) and jungle babbler, Turdoides striatus (n=186). Among 71 species, Alexandrine parakeet, Psittacula eupatria was the only near threatened species: all other species were least concern. Human population pressure, habitat degradation and lack of awareness are the main threats that ultimately will cause reduction in species numbers around the...
Archana Naithani, Pongthep Suwanwaree* and Bartosz Nadolski
...on Myna (Acridotheres tristis)and Red collared dove(Streptopelia tranquebarica).The threatened status of birds given in the checklist is as per IUCN Red List of ThreatenedSpecies2017.The presence of Alexandrine parakeet (Psittacula eupatria) in the study, listed under the category ‘Near Threatened’ in the IUCN Red List, is a matter of concern.The findings of the present study would provide the baseline information about the com...
Lamiaa Elsayed Mokhtar Deef
...>...
Ghazunfar Rashid1, Muhammad Avais1, Syed Saleem Ahmad1, Muhammad Hassan Mushtaq2, Rais Ahmed3,*, Mahboob Ali1, Muhammad Naveed-ul-Haque4, Mehtab Ahmad5, Mumtaz Ali Khan1 and Naimat Ullah Khan6
...m) and Brassica Campestris (Sarson) were estimated twice a day i.e. early morning and afternoon. The fodder samples were collected from different villages of Okara, Pattoki and Ravi areas of the Province Punjab. Nitrate contents of different parts of the fodder plants were estimated qualitatively through the Diphenylamine Filed Test (DFT) and quantitatively by spectrophotometry. The nitrate levels were highest in Jowar, followed by Jai, Shaljam, ...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
Muhammad Zafar*1, Muhammad Khalil Ahmad Khan2, Asmatullah3
Efficacy of methamidophos, fenpropathrin and metasystox against aphid population within the response of Brassica campestris at Multan
...lation on Brassica campestris. The experiment was conducted during October 2013 to April 2014. Crop season following a randomized complete block design (RCBD) with four replications and six treatments in the research farm of Babar Agricultural Farm at Old Shuja Abad Road, Multan. Different insecticides showed different mortality of aphid population. Of the three insecticides tested, methamidophos resulted in more than 80% mortality in the aphid population and ...
Temel Göktürk1,* and Göksel Tozlu2
...ots pine (Pinus sylvestris L.) forest in Artvin - Borçka, Turkey. In order to propose effective control measures, this study was conducted to estimate the effectiveness of two biopesticides against D. pini ile N. sertifer larvae. Different doses of Pyrethrum and Bacillus thuringiensis biopesticides (Spruzit® Neu from 150ml to 600ml/100 l, and DiPel® DF BT 100 to 500 g/100 l) were applied against ...
Muhammad Nasir1,*, Ata-ul-Mohsan2, Shafqat Saeed3, Munir Ahmad2, Muhammad Asif Aziz2 andMunir Ahmad4
Muhammad Nasir1,*, Ata-ul-Mohsan2, Munir Ahmad2, Shafqat Saeed3, Muhammad Asif Aziz2, Muhammad Imran4 and Umer Ayyaz Aslam Sheikh4
...ueens of Bombus terrestris during flight activity period and solitary phase of development. Results showed that survival rate and colony initiation rate of the queen was highest at 28 ºC and 30 ºC. Different parameters in the initial stage of colony growth like pre-oviposition period and emergence timing of the first worker were observed best at 28 and 30 ºC. Number of workers produced in the first brood and in the total lifespan of the c...
Hayat Zada and Ahmad-ur-Rahman Saljoqi*
...pping (Brassica campestris, Brassacicacae), Glycine max, leguminacae), Trifolium alexandrinum, Fabaceae) and Triticum aestivum, Poaceae) in the apple orchard were a substantial effect on the management of C. pomonella (L). Minimum mean fruit drop were recorded for the intercrop Apple + Trifolium (2.87) which were significantly lower than all other intercrops including control (Apple sole). Likewise, minimum percent infestatio...
Syeda Shazia Bokhari*, Ghashia Qayyum, Uzma Rafi and Aisha Waheed Qurashi
...ts of Lumbricus terrestris Isolates were identified through different biochemical tests and morphological characterization. Bacterial strains were screened and those showing positive results for their adherence ability, autoaggregation and coaggregation response were used for the vermicomposting process. Both of these strains were inoculated for vermicomposting and whole of the setup was maintained for 60 days.The results showed that microbes were chara...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
...our aliquots in which tris-citric acid extender (TCA) was supplemented with various concentrations of L-cysteine 0 mM, 0.5 mM, 1.0 mM and 1.5 mM. Cooling and equilibration of the diluted semen was done at 4°C for 4 h subsequently filled in straws at the same temperature and were cooled in liquid nitrogen vapors for 10 min. followed by storage at -196°C for assessment. Progressive motility and viability of spermatozoa were significantly higher in...
Ahmad Ali1,2,* and Aleem Ahmed Khan1
... (Acridotheres tristis), dove (Streptopelia senegalensis) and mice (Mus musculus). While the percentage use of quill, mice, dove, sparrow and myna has been calculated as 66.67%, 46.24%, 46.24%, 34.41% and 21.51%, respectively. Further it was observed that falcon trapping was a teamwork and each team used multiple techniques at the same time during capturing in the field.
...
Muhammad Naeem Khan* and Asad Jan
...gainst Xanthomonas campestris and Pseudomonas aeruginosa causing 12.6±0.54 mm and 20.6±0.53 mm zone of inhibition, respectively which was analogous to that of cefixime, used as standard drug. In case of antifungal activity MCE hindered the growth of Fusarium oxysporum effectively, causing 19.3±0.41 mm zone of inhibition, while the activity of other solvent was moderate. ...
Abid Khan*, Mukhtar Alam and Yousaf Jamal
...a (Rhodopseudomonas palustris, Rhodobacter spaeroides), lactic acid bacteria (Lactobacillus plantarum, Lactobacillus casei, Streptococcus lactis), yeasts (Saccharomyces cerevisae, Candida utilis), actinomycetes (Streptomyces albus, Streptomyces griesus) and fermenting fungi (Aspergillus oryzae, Mucor heimalis). Farm yard manure (FYM) and poultry manure (PM) were used as organic sources of nitrogen. Bio-primed (seeds soaked in 10% BM aqueous solution for 30 min...
Mah Jabeen and Amjad Farooq*
...pus, B. juncea. B. compestris and B. rapa were studied for their relative resistance/susceptibility against aphids, under field conditions for three consecutive crop seasons i.e., 2009-2010, 2010-2011 and 2011-2012. The data on the incidence of aphids were recorded at weekly interval. Host plant susceptibility indices were also calculated. B. napus was found to be susceptible showing maximum population of aphids i.e., 6.80 per 1...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...pecies (S. g. flaviventris) but also other species reported from various countries. Migratory pattern of S. g. gregaria from Africa to Asia and neighboring countries with their potential routes also suggested developing sustainable policy to counter internal as well as external threat. This is the first reportof molecular identification of S. g. grgaria and its association with most prevailed subspecies of Sahara desert in Northern Africa....
Pankaj Kumar Jha1,2*, M. Golam Shahi Alam1 and Farida Yeasmin Bari1
Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes
...ladyl® and home-made tris based) and number of frozen-thawed ram sperm (100 × 106 and 50 × 106 sperm dose) on pregnancy and lambing rate following laparoscopic artificial insemination (LAP-AI) in indigenous ewes in Bangladesh were studied. A total of 78 non- pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; G1 (n = 18; commercia...
Bushra Ismail Khan1, Shamim Akhter1, Sanwal Aslam2* and Rabea Ejaz1
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...culates were mixed in tris citric acid extender having 0.0 (control), 20.0, 25.0 and 30.0 ng/mL of arachidic acid at a temperature of 37°C (>1 mL volume, >0.5 billion per mL conc., >60% motility) and cryopreserved using standard procedures. Percent sperm motility, liveability, plasmalemma integrity and viability were increased in extender (P<0.05) having 20.0 ng/mL of arachidic acid compared to 25.0 ng/mL, 30.0 ng/mL and control. However...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
...(SFE) supplementation in tris Egg-yolk extender on mortality and motility of spermatozoa of Limousin bull (Bos primigenius f. taurus [Linnaeus, 1758]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected and processed according to the protocol at Singosari AI Centre. Semen extender were supplemented with SFE, G0 = 0 % SFE, G1 = 2 % SFE, G2 = 4 % SFE, G3 = 6 % SFE and G4 = 8 % SFE....
Md. Ariful Islam, Md. Badol Ashraf and Md. Mahmudul Alam*
Ramadan, F. Abbas1;H.M.Salem1; M.H. Belal2, and A. M. M. Abd-Alla3*
Eman A. Ahmed1, Osama Y. Shalaby2, Emad F. Dwidar2, Samah A. Mokbel1, Ahmed K. El-Attar1
...uscuta
campestris), insects in the family Cicadellidae (leafhopper, Empoasca decipiens) and in
germinated seeds within the fruit, suggesting mechanical inoculation and seedtransmissible
were not feasible while the positive results were obtained by the other three
techniques for host plants with numerous symptoms obtained later. Transmission electron
microscopy (TEM) of experimentally inoculated sample...
Shuli Zhu, Yaqiu Liu, Weitao Chen, Xinhui Li and Yuefei Li*
..., Distoechodon tumirostris (Peters, 1881), Bangana decora (Peters, 1881), Ptychidio longibarbus (Fang, 1981)] from Liujiang River, China. Fishes were collected from local fish markets that using various fishing gears such as gillnets, castnets and shrimp cages in July and December 2018. The LWRs parameters a and b values for the four species varied 0.008861-0.02018 and 3.041-3.367. New maximum standard lengths for one species...
Tajnees Pirzada1*, Weenghar Ali Chandio1, Mansoor Ali Kalhoro2, Mir Munsif Ali Talpur1, Waheed Ali Mirbahar1, Abdul Ghafar Solangi1, Zulfiquar Ali Jumani1 and Rehana Kerio1
...uated for Brassica campestris seed germination. A simple one-pot method was used to synthesize HA/ZnO NPs involving zinc oxide nanoparticles (ZnO) core (20-35 nm in diameter) and humic acid shell. HA/ZnO NPs were used to investigate the effect on the germination profile of Brassica campestris. Germination profile parameters were measured as root-shoot length, germination index, fresh and dry weight for 15 days. HA/ZnO NPs we...
Roheela Yasmeen1*, Laiba Asif1 and Samia Djeffal2
...vulture (Gyps tenuirostris). The use of the non-steroidal anti-inflammatory drug (NSAID) diclofenac has been linked to this rapid decline in vulture populations. Diclofenac has been in use since 1974 to treat several problems in cattle such as pain during calving, lameness, mastitis, and swelling. The drug was also used to treat diverse problems such as dysmenorrhea, ocular inflammation, rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, and ...
Syeda Shazia Bokhari, Aisha Waheed Qurashi*, Roheela Yasmeen*, Fouzia Yasmeen, Nabeela Nayab, Uzma Rafi
...thworms (Lumbricus terrestris) towards adherence and exopolysaccharides productions. Both productions are important parameters of plant growth promoting bacteria. Earthworms, along with soil sample, were collected from different areas of Lahore. Bacteria were isolated, purified and further characterized morphologically, biochemically and at molecular level. Bacteria after isolation were labelled as EPF1 while by molecular confirmation this bacterial strain was...
Hongbin Li1,2, Lei Fang1, 3 and Lei Cao1,2*
... geese (Anser serrirostris)fitted with telemetry devices from two flyways between Yangtze River Floodplain winter areas and Anadyr (Russia)/Central Arctic breeding grounds. We combined these with high spatial resolution MODIS satellite-derived plant production data to relate the timing of spring migration to that of the green wave index (GWI) at different staging regions and on breeding areas. Results showed that, at stopovers south of 52°N, eastern...
Ji Xu, Zhonghua Liu, Zhenhai Cui and Wenhai Zhao*
...ponins of Tribulus terrestris (GSTT) on chondrocyte injury induced by IL-1β and its possible mechanism. Chondrocytes were isolated from rat knee joints and cultured. Chondrocytes were treated with IL-1β, and chondrocytes were treated with different concentrations of GSTT. ELISA was used to detect the levels of IL-6, TNF-α and IFN-γ. Flow cytometry was used to detect the apoptotic rate. The expressions of Bax, cleaved-caspase3 and Bcl-2 we...
Roshana Mukhtar1, Shaheen Shahzad1*, Sajid Rashid2, Maryam Rozi2, Madiha Rasheed3, Imran Afzal4 and Pakeeza Arzoo Shaiq5
... of Urogen (3-[7, 12, 18-tris (2-carboxyethyl)-3, 8, 13, 17-tetrakis (carboxymethyl) 5, 10, 15, 20, 21, 22, 23, 24-octahydroporphyrin-2-yl] propanoic acid) due to narrowing of domain-I and domain-II (18.46-12.17Å) of UROSL237P as compared to UROSWT. This suggests that UROS L237P mutation may influence heme biosynthesis mechanism through altered Urogen binding mechanism. Therefore, we propose that the newly identified pathogenic missense variant (c.935T&g...
Aamir Ibrahim1, Bingyao Chen1, Hassan Ali2, Imran Ali3, Yang Cao1 and Guang Yang1*
...olphin (Orcaella brevirostris) in South Asia, Baiji (Lipotes vexillifer) in the Yangtze River of China, and the Amazon River dolphin (Inia geoffrensis) in South America, it is very difficult to take photographs of Indus river dolphin. From March 1 to 9 in 2019, a survey was conducted in the area covered in a branch of approximately 70 km of the Indus River from Taunsa barrage (District Muzaffargarh, Tehsil Kott Addu) to just downstream of Ghazi Ghat near Samin...
Atta Ullah*, Nasrullah Khan, Ataur Rahman and Rafi Ullah
...speghol), Tribulus terrestris (Maklindey), and Fagonia indica (Spelaghzaie). Leaves and shoots were frequently utilized as remedies. We conclude from the study that angiosperms flora of the park can be used for different folk purposes and treating gastrointestinal, skin, and blood disorders ailments. In addition, we recommend the sustainable use of the flora to maintain the proper floristic composition in the studied site....
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
Asim Ali Khan1, Muhammad Ahmad Zeshan1, Yasir Iftikhar1*, Mustansar Mubeen1, Maham Sohail1, Sonum Bashir1, Malik Abdul Rehman2 and Muhammad Usman Ghani3
...ease is caused by citrus tristeza virus (CTV) expresses salient symptoms like stem pitting, yellowing of leaves, stunted growth of large, small lateral branches and twigs. The study was carried out to quantify the spatial and temporal distribution of CTV disease in major citrus growing areas of Sargodha district (Sargodha, Kot Momin, and Bhalwal). The samples from the declined orchards were collected based on symptomology and confirmed through ELISA. CTV was d...
Nuzhat Kamal1, Muhammed Sajjad Ansari2*, Bushra Allah Rakha1, Shamim Akhter1, Saima Qadeer1 and Ali Akhter1
... bulls were diluted with tris-citric acid extender supplemented with different concentration of alanine (5, 10, 15 and 20mM), extender without alanine was kept as control. Extended semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. After equilibration, cooled semen was filled in 0.5 ml French straw at 4°C and kept over liquid nitrogen vapors for 10 min. Then straws were plunged into liquid nitrogen (-196°C) for storage. After 24 h ...
I Wayan Lanus Sumadiasa*, Enny Yuliani, Lukman Lukman
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
Amber Khalid1*, Amjad Rashid Kayani1, Muhammad Sajid Nadeem1, Muhammad Mushtaq1, Mirza Azhar Beg1 and Surrya Khanam2
...llowed by Brassica campestris (mustard) and Arachis hypogaea (peanut). Sorghum bicolor (millet) and Vigna radiata (mong bean) was also consumed in small proportions. Pearson chi-square was used to calculate the significance difference among every food item and among seasons. Significant difference (p<0.05) was observed among consumption of different food items and among different seasons. Non- significant difference (p>0.05) was calculated between consum...
Hasanain Jihad Neamah
Hala A. Amin1; A. Barakat2; A.A. Abou Zeid1
...tein (CP) gene or Citrus tristeza virus (CTV) encoded by the expression vector plasmid (Pinpoint™ Xa3, Promega) with a fragment of biotin binding protein (BBP-rCTV-CP) was highly expressed and purified from E. coli cell culture. Antiserum obtained from rabbits after injection with the BBP-rCTV-CP fusion protein showed comparable reactivity in the serological detection of CTV. This antiserum could be used at dilution or 1: 10.000 at the detection stage in...
Fakhra Nazir1*, Sahar Fazal1 and Fakhar-i-Abbas2
...pecies (Urocissa flavirostris, Dendrocitta vagabunda, Corvus splendens, Corvus corax and Corvus macrorhynchos) from three genera of family Corvidae. The nucleotide sequences were submitted to the Barcode of Life Data System (BOLD) and the eligible sequences were assigned the Barcode Index Numbers (BINs). The COI gene sequence data of 8 species of family Corvidae were retrieved from GenBank (NCBI) database and phylogenetic relationship was established in these ...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
... in the basic diluent of tris-aminomethane on the quality of spermatozoa. Bulls aged 3–4 years used in this research. This research used an experimental method to examine the effect of genistein levels in the diluent on the characteristics of spermatozoa during pre-freezing at 4–5 0C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 1...
Komal Javed1, Sohaib Muhammad1*, Zarghaam Khan1, Sehar Fatima1, Hassan Nawaz1, Mahrukh1, Tahira Khalid1 and Shariat Ullah2
...ies each. Brassica campestris, Lathyrus aphaca, Melilotus indicus, Medicago polymorpha, Dicanthium annulatum, Setaria glauca and Solanum nigrum were the only 7 species found in all three crops of wheat, rice and sugarcane having IVI values of 5.86, 6.35, 4.32, 4.93, 4.36, 5.64 and 5.40 respectively. Therophytes, Hemicryptophytes and Chamaephytes were the prominent life form classes of the research area....
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
Rida Ahmad1,2*, Zulfiqar Ali2*, Farkhanda Manzoor1, Usman Ahmad3, Safdar Sidra4, Irfan Zainab2, Muhammad Furqan2, Aliza Batool1,2 and Zaidi Zona2
...Common Myna Acridotheres tristis (RA=8.599), Carrion Crow Corvus corone (7.486), Large-billed Crow Corvus macrorhynchos (6.240). Two threatened bird species Steppe Eagle Aquila nipalensis and Western Tragopan Tragopan melanocephalus were observed. Habitat suitability index (HSI) of former species was maximum in rangelands (0.82) even though it was also observed in six habitats. Furthermore, Western Tragopan was found only in moist temperate habitat with HSI 0....
Misbah Ammanat1, Abdul Qadir1, Zulfiqar Ali2*, Rida Ahmad2,3, Usman Ahmad1, Irfan Zainab2 and Aliza Batool2,3
...ommon Myna (Acridotheres tristis) and Carrion Crow (Corvis corone) with relative abundance 9.36, 6.58 and 5.73 respectively. Out of 215 species, 27 are collision-prone based on published reports or morphology. Natural birds and migratory sub-routes in the study area highlight the study’s significance. Researchers might benefit from this research for similar studies in future developmental projects....
Bakhtawar Soomro* and Shakeel Ahmed Memon
...common myna Acridotheres tristis of District Larkana, Sindh, Pakistan. In all, 20 nematodes (♂♂) were recorded. Present nematode reflects diversification from their congeners in the following charaters viz: body measurement; shape; size and shape of trident; shape of spicules and presence of 13-14 pairs of caudal papillae. On the basis of such morpho-metrical changes this species; Diplotriaena murtazi treated as a new species. This species is dedicated to ...
Aeni Nurlatifah1, Anita Hafid1, Pradita Iustia Sitaresmi1, Santoso Santoso1, Rahma Isatirna Anwar1, Mohammad Firdaus Hudaya1, Diana Andrianita Kusumaningrum1, Nurul Azizah1, Dimar Sari Wahyuni1, Herdis Herdis1, Florentina Bety Indah Lupitasari1, Desiana Ade Mahari1, Muhammad Rizal2
...100 ml into the egg yolk tris extender resulted in the greatest preservation of ram sperm quality (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tris extender resulted in the highest (P<0.05) percentage of motility, percentage of live spermatozoa, and intact plasma membrane. The NEZ group showed better results (P<0.05) than the NEZ group in m...
Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2
...ice addition to egg yolk tris-buffer diluent on the semen quality of Kacang goats at 5°C storage. Semen samples were collected twice a week from a 2-year-old Kacang goat using an artificial vagina. The study design consisted of three treatments; T0, T1, T2 and T3, consisting of 0%, 10%, 15%, and 20 % of sugarcane juice, respectively. The measured parameters included progressive motility, viability, and abnormalities of spermatozoa. The data obtained were a...
Hyun-Su Hwang1, Jae-Kang Lee2, Tae-Kyung Eom2, Seung-Hun Son3, Dong-Ho Lee2, Hyeongyu Ko2 and Shin-Jae Rhim2*
...marsh tits (Poecile palustris), great tits (Parus major), and Eurasian nuthatches (Sitta europaea). In addition, the type of food, temperature, and metabolic energy requirement affected food utilization in marsh tits and great tits. In our study, some evidence was found that wintering birds in temperate zones prefer high-energy food items. Limited food availability during winter affects social hierarchies....
Mazharul A. M. 1, Md. S. Sarwar ,M. Nurullah 2, Keshab K. Adhikary I and M. Rahmatullah 1
...x plants Nyctanthes arbortristis, Swertia chirata, Vitex negundo, Andrographis paniculata, Clerodendrum viscosum and Tinospora cordifolia were used for treatment of viral fevers. Stemona tuberosa was used in case of measles. Moringa oleifera and Azadirachta indica were used to treat chicken pox. Three plants, namely Cyathula prostrata, Amaranthus spinosus. and Calotropis procera were used to treat patients with rabies.
...
E.F. Mohamed1 and I.M. Sabra2
... ToMV on Nicotiana sylvestris. This reduction increased gradually by increasing GA and IAA concentrations. ToMV inhibition percentages induced by GA and IAA varied according to the time of treatment (1, 2, 3, and 4 hours). High percentages of inhibition were recorded for in Vitro treatment. ToMV inhibition of pre-inoculation treatment was higher than that of post-inoculation treatment. IAA was more effective than GA....
ABRAR HUSSAIN1, AQSA KHALIL1, SAIRA ZAHEER1, MUHAMMAD LATEEF2 & MUHAMMAD MANSHA1
...tivity of Tribulus terrestris L. and Ziziphus mauritiana Lam. Whole plant of Tribulus terrestris L. and leaves of Ziziphus mauritiana Lam. were used. The plant materials were dried, ground firmly, macerated in ethanol and then the crude ethanolic extract (CEE) was prepared. Adult motility assay (AMA) was carried out on adult Haemonchus contortus to analyze the anthelmintic efficacy of CEE of the whole plant and the leaves of...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...reg; as a comparison, P0 tris egg yolk (TYE), P1 TYE+MYE 1 mL, P2 TYE+MYE 2 mL. The parameters observed include fresh semen quality, sperm motility, sperm viability, sperm membrane integrity, and recovery rate. The results showed that MYE 2 mL supplementation (P<0.05) affected the quality of sperm motility during equilibration and post-thawing compared to P0 and P1. MYE 2mL supplementation in tris diluent has almost the s...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
Ali Bouzekri1,2*, Souheila Slimani1,2, Meryem Nassar1,2, Souad Zaaboub3 and Nora Sakhraoui1
...justify;">Tribulus terrestris is traditionally used to treat various diseases. The current research aimed to study the protective effects of methanolic extract of Tribulus terrestris (TT) against Spirotetramat (SPT) induced reproductive toxicity in adult male domestic pigeons (Columba livia domestica). For ten consecutive weeks and under an artificial photoperiod (19L: 5D), thirty male pigeons weighing approximately 309,20 &...
Irshad Ur Rehman,SAEED ULLAH KHATTAK,Muhammad Kamran,Jamshaid Ahmad,Abid Ali Khan,Ghosia Lutfullah
Irum Mukhtar,Sobia Mushtaq,Ibatsam Khokhar
Mehreen Samad , Lal Badshah and Shujaul Mulk Khan
Mian Muhammad Shafiq and Hussain Bux Bhaagat
...>Marmaronetta angustisvostris) breed in these lakes in May-July. According to the data Deh Akro II wildlife sanctuary provides good wintering ground to the migratory waterfowls, as there was least disturbance. Moreover, this lake is a good habitat for the crocodiles as 200 -250 Crocodiles of different sizes were recorded in these lakes.
Mohammad Zubair Sulemani* Mohammad Safdar Baloch** and Khalid Abdullah**
...ssica (Brassica compestris) and taramira (Eruca sativa). Seed germination of all sevel crops was significantly and negatively affected due to the application of extracts as compared to tap water treatment. Highest adjusted reduction in germination (89.67%) was recorded in Sorghum followed by Taramira (85.36%), while maize was least affected (60%).
Keywords: Allelochemicals, kiker, Acacia nilotica, field crops, germination.
...
Aashaer Farooq and Zakaullah
...ds. Xanthomanas campestris was the only bacterium isolated from the non-treated seeds....
S. M. Raidh, M. K. Hossain and S. Akhtar
...and Chickrassia tabulatris. Five different sizes of polybags were tried for three months time in the nursery. The parameters of seedlings like total height, collar diameter, root length, root diameter, dry weight of root and shoot etc. were
measured at the end of the experiment. The finding showed that the polybags of T4 treatment (30.5 x 15 cm size) produced quality seedlings of both the species within three months time and the size may be used in qua...
Fazli Wahid Khan, Pazir Gul and Abdul Ghaffar Marwat
...ruba, and Q. palustris. It is concluded that the oil is non-drying in nature and is fit for edible purposes as it has non-toxic compounds and can be purified cheaply. The seeds have been analysed for protein, arbohydrates and ash and the results indicate their utility as poultry and livestock feed.
...
Mohammad Shahid and Abdul Qayyum
..., apple (Malus
sylvestris ), loquat (Eriobotrya japonica), clovers
(Trifolium spp.),serson (Brassica campestris), maize
(Zea mays),dates (Phoenix spp.), citrus fruit (all
spp.), ber (Zizphus jujube), guava (Psidium guyava) and
shain (Plectranthus rugosus)were recorded as major
sources for the production of surplus honey with five
major honey-flows in a yea...
Mamoona Arooj1, Bilal Ahmad Khan*1, Muhammad Ather Nadeem1, Muhammad Mansoor
Javaid1, Erum Rashid2, Muhammad Saleem Jilani3, Jamshahid Qamar1, Faryal Ali4, Sadaf
Javeria5, Muhammad Faisal4
...growth of Tribulus terrestris. Pot
experiments were conducted at Agronomic Research Area, collage of Agriculture, University
of Sargodha, during 2019. Six different concentrations of atrazine [0, 5, 10, 20, 40 and 80 g
a.i. ha-1] were applied as post emergence herbicides at 3- 4 leaves stage of the weeds
under two different water regimes (60 % and 100 % water concentrations). Results
revealed that inc...
Qurat-ul-Ain Ijaz1, Amna Sulaman1, Dost Muhammad Baloch2, Muhammad Shafi2, Faiz Muhammad1* and Shahnaz Rashid1
...Nerita albicilla, Nerita tristis, Ischnochiton australis, Astralium tentorriformis). T our knowledge among them three are news records (A. tentoriiformes, Ischnochiton australis and, Nerita tristis). The highest evolutionary divergence within the family of Nacellidae (0.917) followed by Turbinidae (0.169) and Neritidae (0.100) was noted. The results of this study will be helpful to taxonomists because meager attention was gi...
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2,
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2,
Florentina Bety Indah Lupitasari2, Maman Surachman2,
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
Abdullah Channo1,2*, Asmatullah Kaka1, Akeel Ahmed Memon1, Mool Chand Malhi3, Muhammad Bakhsh4, Qudratullah Kalwar5, Shakeel Ahmed Tunio6 and Muhammad Ibrahim Panhwar7
Safaa Sabbar Atiyah1*, Ali Abdullah Zuairi Al-Sadoon2, Shifaa Kadhum Jieish2
Aulia Puspita Anugra Yekti, Ani Atul Arif, Putri Utami, Habib Asshidiq Syah, Anggita Dian Pramudhita, Rr. Ani Rizqianti, Trinil Susilawati*
... PDGC 10 fractions using tris aminomethane diluent + 20% egg yolk. T2: sexing PDGC 5 fractions using Andromed® diluent. T43: sexing PDGC 10 fractions using tris aminomethane diluent + 20% egg yolk. Parameters measured include motility, viability, abnormalities, concentration, Total motile sperm, and proportion of sperm. The results of the analysis of variance showed significant differences in the upper layer for motility...
Siew Ing Nguang, Nur Syafiqah Binti Zainal, Ahmad Mukhlis Bin Hafas, Hou Chew Ha, Connie Fay Komilus, and Asmad Kari*
Dietary Habits of Indian Gerbil Tatera indica Inhabiting Agro-Ecosystem of Pothwar Plateau, Pakistan
Anum Razzaq*, Tariq Mahmood, Ammara Saman, Ammara Baig, Nadeem Munawar and Muhammad Farooq
... (Maize), Brassica campestris (Mustard) etc. The summer diet (non-crop season) was comparatively less diversified than the diet of the cropping season (spring) and there was a significant difference in the diets of this rat species during cropping and non-cropping period. The gerbil also supplemented its diet with insects (animal matters) in higher quantities highlighting its positive role as biological control of insect pests of croplands. Analysis of Varianc...
Talha Shafique1, Mustansar Mubeen2, Yasir Iftikhar2, Qaiser Shakeel3*, Muhammad Ahmad Zeshan2, Haseeb Ahmed Lalika4, Malik Abd-ur-Rehman5, Atta ur Rehman2, Muhammad Irfan Zafar6 and Farwa Seemab2
...current status of citrus tristeza virus (CTV) in three tehsils of the Sargodha district: Kot Momin, Sargodha, and Bhalwal. The incidence and severity of CTV were evaluated using three citrus varieties: Musambi, Feutrel’s Early, and Kinnow. Disease incidence was calculated using the ELISA test, while disease severity was determined by counting the number of pits per square inch. The results revealed significant variation in CTV incidence and severity acro...
Arshad Javaid1*, Freeha Anjum1, Aneela Anwar2, Mahrukh Asif1, Sadia Ahmad1 and Malik F.H. Ferdosi3
...inal herb Tribulus terrestris was assessed for its antifungal activity against this fungal pathogen. Roots of T. terretris were collected from Lahore, Pakistan and shade dried. The dried and crushed roots were extracted in methanol for two weeks and the effect of 1, 2, 3, 4 and 5% concentrations of the extract was checked in vitro against P. oryzae using malt extract broth as a growth medium. All the applied concentrations o...
Lalu Ahmad Zaenuri*, Rodiah Rodiah
...ddition of 8 ml (v/v) of tris-albumin-based extender has a significant preservative effect on all three indicators: progressive motility, viability, and morphology of spermatozoa, making it suitable for artificial insemination (AI) for up to 10 hours after dilution and storage at 27°C. It would be advantageous to conduct more comprehensive research to investigate the effects of varying FF concentrations in different storage conditions, such as room tempera...
