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Halide Nihal Açikgöz1*, Serdal Kenan Köse2 and Ali Açikgöz3

 

 

...ract blowflies. Eggs and larvae of the flies that oviposited on the liver were raised and identified. Six different indices were used to determine diversity (Shannon-Wiener [H’], Simpson Dominance [Sd], Simpson Diversity [S], Margalef Species Richness Indices, Pielou’s Evenness [EH], and Simpson Resiprocal Indices). Calliphora vicina (Robineau-Desvoidy), Calliphora vomitoria (Linnaeus), Lucilia sericata (Meigen) and Lucilia richardsi (Collin) (Dipt...

Ambreena Hafiz1, Tanzeela Riaz2 and Farah Rauf Shakoori1*

...ofile of 4th, 6th instar larvae and adult beetles of deltamethrin-resistant populations of T. granairum collected from wheat godowns of Gujranwala, Okara and D.G khan has been studied in current investigation. The toxicological data generated from deltamethrin-resistant populations was compared with deltamethrin-susceptible population that has not been exposed to any insecticide since thirteen years. From analysis of metabolic profile, it was found that solubl...

 Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5

...todes did migrate to the larvae and caused some infection after 2, 3 and 4 days. Greatest invasion took place with the highest dose (80,000) with 8 IJ successfully finding and penetrating the larvae. A positive correlation was observed between dose and time and invasion of the host. It is concluded that application of EPN in cadavers may be appropriate in Pakistan because of the non-availability of industrially produced isol...

Marryam Bakhtawar1, Qamar Saeed1* and Naeem Iqbal2

...ival of lady bird beetle larvae on mustard aphid (76.6%) followed by on grain moth’s eggs (20%), immature mealybugs (6%) and the least survival on honey. These findings can lead to the discovery of new artificial diets for the C. undecimpunctata for the mass release in fields in order to biologically control a number of pests for an eco-friendly pest control system.

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Muhammad Raheel1,*, Nazir Javed2, Sajid Aleem Khan2, Hafiz Muhammad Aatif3 and Sohail Ahmed4

...d on Galleria mellonella larvae. The native species included Steinernema asiaticum and Heterorhabditis indica whereas exotic species were S. feltiae and H. bacteriophora. G. mellonella larvae were exposed to 300 IJs of each species. After inoculation at different temperatures, the reproductive potential of EPNs increased with increasing temperature and was found to be the best at 25oC. No EPNs species could reproduce at 5oC....
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...l COI sequences from the larvae show greater than 99% sequence identity to those from adults, but some larvae share the same haplotypes as adults. These findings clearly indicate that the larvae collected from sea waters around an oyster farm belong to P. websteri, the same species as the adult worms collected from the oyster Crassostrea hongkongensis in that locality. Two po...
Farkhanda Manzoor1, Iram Amin2,*, Muhammad Shahid2,Samia Afzal2,Hania Ramzan1, Tahir Rehman Samiullah2 and Muhammad Idrees3
...ion of dengue virus from larvae of Aedes aegypti (L) and Aedes albopictus (Skuse) by using PCR. During this study, larvae of A. aegypti and A. albopictus were collected from different localities of Lahore during May to September of 2012 to 2014. RNA extracted from each mosquito larvae pool was tested by nested PCR for detection of dengue virus. Out of total 50 p...
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
...43%) adults (n=1050) and larvae (n=550). The samples were collected from four (Shuba Bazaar, Tarnab Farm, Wazir Bagh and Hayat Abad) dengue suspected locations of district Peshawar, Khyber Pakhtunkhwa (KPK), Pakistan. A semi-nested multiplex PCR was used to amplify the cDNA of the four dengue serotypes. Six out of 35 (17.14%) adult mosquito pools were found positive for dengue virus with type-2 (77.77 %) and type-3 (11.11 %) while a single (11.11%) pool of con...
Tanzeela Riaz1, Farah Rauf Shakoori2* and Syed Shahid Ali3
...p; 6th instar larvae and adult beetles of phosphine-tolerant populations (previously exposed to phosphine for 15 years) of wheat grain pest, Trogoderma granarium collected from various storage facilities of Punjab, Pakistan viz., Mandi Bahauddin-I, Mandi Bahauddin-II, Gujrat, Gujranwala and Sargodha. The activities of all esterases tested were significantly increased in all field collected phosphine-tolerant populations when compared w...
Sehrish Rasool1, Zain ul Abdin1,*, Saqi Kosar Abbas2, Sumra Ashraf1, Maryam Anwer1, Atif Manzoor1, Muhammad Tahir1 and Hoor Shaina1
...of wasp eggs (4 eggs per larvae) on the hosts larvae and minimum number of adult wasps was recorded from the hosts G. mellonella 44 ±1.15 (se), and S. litura (39 ±0.57 (se) by placing the highest number of wasp eggs (20 eggs per larvae) on the hosts larvae. Likewise mean width of the head of adult wasp emerged from G. mello...

Neerja Agrawal, Mukesh Srivastava, Akhilesh Tripathi and Amrendra Singh

Survey and monitoring of pests, parasites and predators of pulse crops in central and eastern Uttar Pradesh
...y feeding on H. armigera larvae. In pigeonpea crop, mainly H. armigera, leaf webber Grapholita (Cydia) critica, Myllocerus spp, spotted pod borer Maruca testulalis, plume moth Exelastis atomosa, tur pod bug Clavigralla gibbosa, jassid Amrsca bigutulta, termite Odontotermes spp, green bug Nezara viridula, tur podfly Melanogromyza obtusa, blue butterfly Lampides boeticus, cow bug Tricentrus bicolor and Pentatomorpha bug were recorded while on lentil crop, black ...

Nilesh Suresh Gole and Bijan Kumar Das

Biology of Dichromia sagitta (Fabricius) (Noctuidae: Lepidoptera), a serious pest of Indian ipecac, Tylophora indica
...agitta on T. indica. The larvae were active almost throughout the year, fed voraciously on leaves and flowers causing extensive damage to T. indica during June and December. Eggs were laid singly or in batch of 2-3 on younger leaves (mostly on under surface) or on young shoot tips. Life cycle was completed in 41-46 days during January- February and 26-32 days during June-July. There were several overlapping generations in a year. Descriptions of different life...

S. Patra, V.W. Dhote, SK F. Alam, B.C. Das, M.L. Chatterjee and A. Samanta

Population dynamics of major insect pests and their natural enemies on cabbage under new alluvial zone of West Bengal
...uary with13.60 and 14.33 larvae /plant during 2011-12 and 2012-13 respectively. Cabbage aphid reached its peak on 9th February (14.17 aphids/inch2 leaf) and 16th February (11.03 aphids/inch2 leaf) of 2011-12 and 2012-13, respectively. Highest parasitized larvae of diamond back moth by Cotesia plutellae were found on 15th and 8th March with 10.42 and 10.50% larval parasitisation during both the seasons, respectively, whereas ...

Manidipa Chowdhury 

Incidence of saw fly, Athalia lugens proxima Klug. as influenced by level of irrigation and fertilizers on mustard
...saw fly population (0.26 larvae/plant) was recorded on the crop grown without irrigation and medium level of fertilizers (60:30:30 Kg NPK ha-1) while lowest population level (0.10 larvae/plant) was observed at highest level of irrigation (three) coupled with medium level of fertilizers (60:30:30 kg NPK ha-1) and two irrigations coupled with lowest level of fertilizers (40:20:20 Kg NPK ha-1).
 

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Muzammil Sattar
...he F1 progeny larvae that developed from adults consuming (Nu lure 5ml) diet had the shortest larval and pupal periods and complete all developmental stages in shortest period of time. Insects reared on different concentrations of proteins. Insects rear on different diets compared on the basis of egg to adult survival, female (%) and pupal weight (g) with low and high protein concentrations.
...
Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3

 

...a wide range of bollworm larvae in both agricultural and horticultural crpos. Flubendiamide is a new chemistry insecticide which also controls bollworm effectively and relatively safer for natural enemies and beneficial arthropods. Present investigations were carried out to determine their impact on larval mortality, pupal and adult emergence of 2nd and 4th instar larvae of Spodoptera litura F. u...
Ayşe Gül Harlıoğlu*
...s, eyed embryo, yolk-sac larvae and absorbed yolk-sac larvae were investigated in rainbow trout(O. mykiss). The polyunsaturated fatty acids (PUFA) were found the highest followed by monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA) in the unfertilized eggs. On the other hand, PUFA was the highest followed by SFA and MUFA in the semen. The proportions of the some fatty acids, such as C14:0, C16:1n-7, C...
Asim Gulzar1,*, Ather Maqsood1, Munir Ahmed1, Muhammad Tariq1, Muhammad Ali2 and Rahmatullah Qureshi3
...aluated on second instar larvae of H. armigera under laboratory studies for toxicity, sublethal and antifeedant effect. The result showed that ethanol based extract was the most effective to control the H. armigera followed by the ethyl acetate. The sublethal concentration of ethanol based extract of C. colocynthis increased larval and pupal duration as compared with control. The percent pupation, adult emergence and pupal weight de...

Imran Ali Rajput1*, Tajwer Sultana Syed1, Ghulam Hussain Abro1, Imran Khatri1 and Abdul Mubeen Lodhi2 

Saleh S. Alhewairini
... 62, 82 and 100% whereas larvae mortality was 72, 77 and 100% after 1, 24 and 48 h, respectively. Alive adult and larvae were completely paralyzed after 24 hours. There was significant difference between 2 treatments, 1 and 24 h, in both adult and larvae. The mortality of both adult and larvae was 100% after 48 hours of exposure to oxamyl. In the field, ...
Tanzeela Riaz1, Farah Rauf Shakoori2,* and Syed Shahid Ali3
...nd 6th instar larvae and adult beetles of phosphine-tolerant populations were evaluated with reference to phosphine-susceptible population as a control. The activities of NADPH-CPR, ethylmorphine-N demethylase, aniline 4-hydroxylase and soluble protein contents were significantly increased in all field collected phosphine-tolerant populations with reference to phosphine-susceptible population. Among developmental stages, the 4th instar

Bashir Ahmad1*, Ahmad-Ur-Rahman Saljoqi1, Hayad Zada2, Shahid Sattar1, Toheed Iqbal3, Saddam Hussain1 and Muhammad Saeed4 

...pulation was 2.1 and 2.0 larvae and pupae per plant in the year 2012 and 2013 respectively. The population increased in September to highest number of larvae and pupae per plant (8.25 and 8.4) were recorded in the years 2012 and 2013 respectively. Then population declined from September to November 3.5 and 3.1 larvae and pupae was recorded per plant in both years as the lowest number. In t...
Ali Murad Rahoo,1,2,* Tariq Mukhtar,1,3 Shoukat Ibrahim Abro,4 Barkat Ali Bughio5 and Rehana Kanwal Rahoo6
...>Galleria mellonella larvae. The production of infective juveniles (IJ) in G. mellonella was significantly affected by nematode species. Significantly higher numbers of IJ were produced by Heterorhabditid species than Steinernematid species in the cadaver. The production of IJ was the maximum in the case of H. bacteriophora which was not statistically different form H. indica. Minimum IJ were produced by S. feltiae. The IJ produ...

 Mohammad Siddique Munawar, Shazia Raja, Elizabeth Stephen Waghchoure* and Muhammad Barkat**

... bacterium Paenibacillus larvae is a serious disease of honey bees worldwide that inflicts considerable economic losses to beekeepers. All work was undertaken in a commercial apiary that showed visual signs of AFB infection. Twenty four hives positive for AFB were identified from the apiary (4 colonies in each replicate). Half of the colonies were treated with shook swarm method while other half were left without any treatment. The treated colonies showed sign...
Jaime Molina-Ochoa1, 2, 6,*, Edelmira Galindo-Velasco2, Ana María Rosales-Gutiérrez2, Martín González-Ramírez3, Roberto Lezama-Gutiérrez3, Wilberth Chan-Cupul3, Steven R. Skoda4, Muhammad Irfan Ullah5, Luis Jorge García-Márquez2 and  John E. Foster6
...s were cultivated in GWM larvae, and the emerging infective juveniles (IJs) were collected from White traps, and pooled in canted neck vented plastic flasks. The susceptibility of an important livestock arthropod, the cattle tick, Rhipicephalus microplus Canestrinito JMO71 was determined. Ticks were manually collected from cross-bred beef cattle, and then the heterorhabditid nematodes assayed against these ticks using the Petri dish assay. Concentration...
Hina Safdar1,*, Nazir Javed1, Sajid Aleem Khan1 and Muhammad Arshad2
...plied at 1000 IJs/ml and larvae maintained at 25°C. Mortality was recorded upto four days. Both species of entomopathogenic nematodes proved effective against all larval instars. Maximum mortality was observed in 2nd and 5th larval instar. After the fourth day 100% mortality was observed in all larval instar. Mortality was increased with an increase in exposure time. Nematodes were harvested using White Traps. Multiplication of entomo...
Xia-Lin Zheng1, Pan Wang2, Jun-Yan Liu1, Yu-Jing Zhang1, Jun Li1 and Wen Lu1,*
...he 4th instar larvae, pupae and adults survived in January, in which their supercooling points were -12.79 ± 0.67 °C, -19.92 ± 0.66 °C and -21.23 ± 0.99 °C, respectively. Body water contents of these stages in B. macroscopa have no negative or positive correlation with the supercooling points. Supercooling points of these stages are lower than the air temperature during the winter, which suggested that these s...

 Habib Iqbal Javed, Ashiq Saleem, Javed Fateh, Mozammil Hussain, Naheed Akhtar and Shamim-ul-Sibtain Shah*

DEVELOPMENT OF RESISTANT MAIZE GERMPLASM
...on with 15 newly hatched larvae per plant every time. Despite of double artificial infestation, 40.0-66.7% plants survived in the resistant germplasm but none survived in susceptible genotypes. Under natural infestation, 5 progenies had no damage while other showed 10.0-22.9% damage with intensity of 2-3 in resistant germplasm. The susceptible germplasm showed 30.0-66.6% infestation with intensity of 5-8. The dead hearts in resistant and susceptible germplasm ...

 Maria Rauf*, Ehsan-ul-Haq**, Javed Khan**, Abdul Rehman***, Waseem Ahmad Gillani* and Asrar Ali**

BIOLOGY AND PREDATORY POTENTIAL OF COCCINELLA SEPTEMPUNCTATA LINN. ON SCHIZAPHIS GRAMINUM APHID UNDER CONTROLLED CONDITIONS
...5.0 and 667.8 aphids per larvae. The results indicated that with increasing temperature, developmental duration decreases significantly. The pupal developmental duration was 14.0, 9.2 and 5.2 days, respectively which are significantly different from each other. The adult male and female longevity were 44.7, 37.7, 30.0 and 60.3, 58.9 and 43.7 days. Fecundity rate of females were 123.5, 251.5 and 293.2 eggs per female, respectively. This indicates that adult mal...

 Muhammad Tayyab Mudassar*, Ehsan-ul-Haq*, Muhammad Naeemullah**, Muhammad Shakeel*, Muhammad Ashfaque*, Asmat un Nisa* and Javed Khan*

ECONOMIC REARING OF CHRYSOPERLA CARNEA (STEPHENS) (NEUROPTERA: CHRYSOPIDAE) IN INSECTARY
...umption of 100 C. carnea larvae was 3.617g of Sitotroga cerealella eggs and 1.793g of Phenacoccus solenopsis crawlers. Adult emergence rate was significantly higher when provided with S. cerealella eggs, which was more economical than that of P. solenopsis crawlers. For efficient and quality production of C. carnea in laboratory using available and inexpensive equipment to produce the healthy surplus of this predator for experiment and small holding field rele...

 Javed Khan*, Ehsan-ul-Haq*, Habib Iqbal Javed*, Tariq Mahmood*,
Awais Rasool*, Naheed Akhtar and Saleem Abid**

BIOLOGICAL PARAMETERS AND PREDATORY POTENTIAL OF CHRYSOPERLA CARNEA (NEUROPTERA: CHRYSOPIDAE) FEEDING ON WHEAT APHID SCHIZAPHIS GRAMINUM (HEMIPTERA: APHIDIDAE) UNDER LABORATORY CONDITIONS
... second and third instar larvae were 3.2 ± 0.13, 4.0±0.21 and 4.8±0.25 days, respectively. The total larval developmental duration was 12.0 ±0.67 days with 85.05% survival rate of larvae. The larval predatory potential was 414.6±0.05 aphid per larvae. The total duration and survival rate from egg to adult emergence was 24.6± 0.06 days with 73% surv...

 Gul Zamin Khan*, Imtiaz Ali Khan**, Inamullah Khan* and Mian Inayatullah**

OUTDOOR BREEDING OF MOSQUITO SPECIES AND ITS POTENTIAL EPIDEMIOLOGICAL IMPLICATIONS IN KHYBER PAKHTUNKHWA

 Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**

BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...leoptera: Tenebrionidae) larvae in the laboratory. The objective was to examine changes in production or activities of carboxylesterase (CE), total esterases (TE), alpha-amylase, glucoamylase, alkaline phosphatase (AkP), acidic phosphatase (AcP), total protein, soluble protein and free amino acids (FAA). The sixth instar larvae of T. castaneum were released and exposed for 48h without food on abamectin treated glass petri di...
Dachuan Cheng1,2, Md Mahbubul Hassan3, Zhenhua Ma1, 2, 3,*, Qibin Yang1 and Jian G. Qin3
...tjanus erythropterus larvae and juveniles from hatching to 36 day-post hatching (DPH). Mandible, ceratobranchial, cleithrum and gill arches were the initial skeletal structures appeared at 3 DPH that supported the vital life functions such as feeding and respiration. Ossification of premaxilla and maxilla and dentary started at 3.21 ± 0.25 mm (9 DPH), and completed at 5.91 ± 0.34 mm (18 DPH). The head skeleton formation completed at 22.35 &pl...
Rabia Faiz and Dilara A. Bukhari*
...st 3rd instar larvae of mosquito. Larval mortality was noted after 24 h and GCU B.t. 4 (400± 1.15) was found to be the most toxic against 3rd instar larvae of mosquitoes. Spores LC50 values of other isolates were 451± 0.90 (GCU B.t. 1), 511± 0.85 (GCU B.t. 2), 525± .013 (GCU B.t. 5), 582± 0.66 (GCU B.t. 3) and 850± 0...
Mahmoud M. Al-Azzazy1,2 and Saleh S. Alhewairini1,*
...hing. The percentages of larvae hatching from eggs of A. lycopersici were 73.42, 56.92, 25.41 and 17.97 under greenhouse conditions and 68, 53.14, 23.51 and 15.18 under laboratory conditions at 1000, 2000, 3000 and 4000ppm of Huwa-San TR50 after one week of treatment compared with the control (well water), respectively. The application of Huwa-San TR50 showed a promising safety profile when compared to other acaricides, as it spared predatory mites at c...
Ali Murad Rahoo1,*, Tariq Mukhtar2, Ali Murad Jakhar3, Rehana Kanwal Rahoo3
...ecovery from cadavers of larvae that had been exposed to nematodes for longer periods.
...
Felicia NkechiEkeh*, Ifeanyi Oscar Ndimkaoha Aguzie, Joy Ihuoma Nzei, Chinenye Maria-Goretti Ohanu, Godwin Ikechukwu Ngwu and ChukwudiebubeUgolo
...ed fish infested with 20 larvae, 20 adults and eggs of D. maculatus. Groups A – C were exposed to 8, 12 and 15% ( w/w) of extract per kilogram of fish respectively. Observation for mortality and larval emergence was made every 3 days and cumulative taking at the end of 28 days. Mortality of adult D. maculatus was higher in 8, 12 and 15% (w/w) of P. guineense and 15% (w/w) of C. annum than control. Both extracts significantly e...
Muhammad Khalid Mukhtar1,*, Hummaira Iqbal1, Hafiz Muhammad Tahir2, Haseena Ghulam Muhammad1 and Muhammad Irfan1
...ted spiders were offered larvae of Drosophila melanogaster in different densities. C. citricola increased prey consumption as the prey density was increased. Insecticide treated C. citricola consumed less prey as compared to control. Effects of chlorpyrifos on the prey consumption of C. citricola were more drastic than bifenthrin. It was also recorded that at low prey density, predation by C. citricola was delayed as compared...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
...nd 6th instar larvae of a stored product pest, Trogoderma granarium. The LC50 values of λ-cyhalothrin for 4th and 6th instar larvae of Lahore population was 15.93 and 13.76ppm respectively, while 19.07 and 16.21ppm were for the 4th and 6th instar larvae of Gujranwala population, respectively. The resistance rat...
Ambrina Hafiz1, Tanzeela Riaz2 and Farah Rauf Shakoori1,*

 

...>, 6th instar larvae and adult beetles of deltamethrin-resistant populations’ viz., Gujranwala, Okara and D.G. Khan was significantly increased as compared to susceptible population of T. granarium (population never exposed to any kind of insecticide/fumigant since 2001). Different developmental stages possessed different levels of esterase activities. Based on the level of activities the adult beetles were more susceptible to de...
Wael S. El-Tohamy1,*, Russell R. Hopcroft2 and Nagwa E.M. Abdel Aziz3
...le River. Meroplanktonic larvae were the major component representing 39.4% of the total zooplankton abundance. Copepods and their larval stages contributed 36.2%. Rotifers ranked the third important group (12.6%). Protozoa contributed 10.6% of the total community. According to the Canonical Correspondence Analysis (CCA), the variations in the species data were significantly (P< 0.05) related to salinity, temperature, phosphate concentration and phytoplankt...
Sukirno Sukirno1,2, Muhammad Tufail1,3,*, Khawaja Ghulam Rasool1Said El Salamouny1, 4, Koko Dwi Sutanto1 and Abdulrahman Saad Aldawood1
...sub>for the first instar larvae were 1.59 x 103 PIB and 4.91 x 107 PIB, respectively. Whereas, these values for the second instar larvae were 4.99 x 106 PIB and 1.06 x 108 PIB, correspondingly. The field experiment data indicated that the mortality of the first instar larvae was highest at day 0 of the sunlight exposure. In virus alone treatments...
Mudassar Javed1,Muhammad Zeeshan Majeed1,*, Muhammad Sufyan1, Sajjad Ali2 and Muhammad Afzal1
... number of lepidopterous larvae in plots treated with the synthetic insecticides was 1.87 and 2.12 larvae/plant in 2015 and 2016, respectively, compared with 6.5 larvae/plant in untreated control plots. For the botanicals, an average of 2.57 and 2.92 larvae/plant was recorded in 2015 and 2016 respectively, compared with 4.12 in untreated control plots. A...
Farah Rauf Shakoori1,*, Tanzeela Riaz2, Uzma Ramzan1, Anum Feroz1 and Abdul Rauf Shakoori2,3,*
...nd 6th instar larvae of a stored grain pest, Trogoderma granarium. The LC50 values of esfenvalerate for 4th and 6th instar larvae of Phosphine and deltamethrin-susceptible population was 34.29 and 28.05ppm, respectively, while 39.30 and 32.67ppm were for the 4th and 6th instar larvae of Phosphine and deltamethrin-tole...
Nevran Eylem Akman Gündüz1,* and Özgür Özcan2
...ed. G. mellonella larvae were fed with a synthetic diet. The effects of GA3 on the hemolymph content of these larvae were tested through a diet incorporation assay. Different concentrations (2, 5, 10, 50, 100, 200, 500 and 1000 mg/L) of GA3 were added to the synthetic diet and protein, lipid, carbohydrate and glycogen levels in the hemolymph were evaluated for the GA3 concentrations. T...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
... collected from infected larvae fed on natural cotton crops. The NPV was isolated from the larvae and viral occlusion bodies (VOBs) were detected using a light microscope. The toxicity of native isolates against S. litura also studied by testing different concentrations (1 x 102 POB (Polyhedral occlusion bodies) mL-1- 1 x 1010 POB mL-1) from the occlusion bodies produced fro...
Dilara A. Bukhari1,*, Naureen Fatima1 and Abdul Rehman2

 

...to 3rd instar larvae of mosquito, Aedes aegypti. Among seven B.t. isolates, three isolates NF5 B.t. 7.2, NF1 B.t. 1.1 and NF2 B.t. 4.2 were found most toxic which were isolated from moist soil containing decaying cattle waste, dry waste animal dung and leaf litter soil, respectively. So, these isolates have a great potential to grow into a biopesticidal formulation for control of mosquitoes.
...
Ali Murad Rahoo1, Tariq Mukhtar2,*, Barkat Ali Bughio3 and Rehana Kanwal Rahoo4
... in G. mellonella larvae of different sizes. The size of G. mellonella had significant effect on the emergence of IJ. Significantly greater numbers of IJ both of H. bacteriophora and S. feltiae emerged from large sized G. mellonella than medium and small sized and the emergence was found to be positively correlated with the size of the host. It was also observed that emergence of IJ of H. bacteriophora was significantl...
Saleh S. Alhewairini
...vely. The percentages of larvae hatching from eggs of O. afrasiaticus were 97.20, 94.62 and 85.53%; 94.70, 93.32 and 84.86%; 54.01, 57.93 and 46.21% after one week of exposure to QRD, HRD and RD of oxamyl only and with Injo200 or 1000ppm of Huwa-SanTR50, respectively. Adding 1000ppm of Huwa-SanTR50 to QRD, HRD and RD of oxamyl was found to significantly increase the toxicity of oxamyl to all developmental stages of O. afrasiaticus, whereas, the R...

 Tanzeela Riaz*†, Farah Rauf Shakoori, Syed Shahid Ali

Toxicity of phosphine against tolerant and susceptible populations of Trogoderma granarium collected from Punjab, Pakistan
...align: justify;"> instar larvae and adult beetle in phosphine resistant populations viz., Gujranwala, Mandi Bahauddin-I (M.B.Din-I), Mandi Bahauddin-II (M.B.Din-II),Gujrat and Sargodha and a susceptible population of T. granarium. The 4<...
Chao Zhao1,2, Juan Feng1, Haidong Xu1, Lihua Qiu1,2,*, Qibin Yang1, Zhenhua Ma1 and Jian G. Qin3
...f Penaeus monodon larvae and bacteria communities in the rearing tanks. The survival of P. monodon in the 36 h experimental period was not significantly affected by the water pretreatment (P > 0.05). However, the bacteria communities were significantly affected by water pretreatment (P < 0.05). Heterotrophic bacteria and vibro counts in the formalin treatment were significantly lower than UV treatment and untreated group (P...
Ghulam Ali1,3, Wopke van der Werf2 and Just M. Vlak1,
...ltNPV-Pak-BNG). Infected larvae with final polyhedrosis (disease resulting in dissolution of larval tissues and accumulation of viral occlusion bodies) exhibited reduced food intake and weight gain relative to uninfected larvae. The unexposed larvae and the larvae that were exposed but survived exhibited the same food consumption and weight gain. This st...
Muhammad Asad Saleem1,*, Mirza Abdul Qayyum1, Mudssar Ali1, Muhammad Amin2, Muhammad Tayyab1,3 and Sumaira Maqsood4
...n in size was found when larvae were treated with integrated effect of Bb and Nit. Depending on the lethal of treatment, development duration of RPW was disturbed. Integration of Bb and Nit delays the development and diet uptake in RPW which can be used for agent of control for this cryptic insect.
...
Rahat Afza, Muhammad Afzal, Muhammad Zeeshan Majeed and Muhammad Asam Riaz*
...t in the absence of prey larvae of C. septempunctata exhibited more predation on C. transversalis while the predation of C. septempunctata on C. transversalis was relatively less in the presence of prey. The larvae of C. septempunctata were found stronger and competitive than larvae and adults of C. transversalis during IGP. Higher IGP of C. sep...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Saleem Haider1, Hafiz Azhar Ali Khan1, Muhammad Ali1, Muhammad Ashfaq1, Muhammad A. Aqueel2 and Muhammad Irfan Ullah2
...nsive range of bollworms larvae in both Agricultural and Horticultural crops. Spodoptera litura is a commercial pest of diverse field crops comprising cauliflower (Brassica oleracea var. botrytis). Current inspections were carried out to determine their impact on larval mortality, pupal and adult emergence by exposing three field strains of S. litura (Faisalabad, Chiniot and Sargodha), under laboratory conditions. Both NPV and Bt...
Ali Murad Rahoo1,*, Tariq Mukhtar2, Barkat Ali Bughio3 and Rehana Kanwal Rahoo4
...rom G. mellonella larvae were 272,600 from T. molitor medium were 194,600 and in T. molitor small were 21,900. Whereas, emergence of the mean numbers of S. feltiae IJ emerging from G. mellonella were 136,000 from T. molitor medium were 51,200 and in T. molitor small were 12,940, respectively. G. mellonella was found to be more susceptible host than T. molitor. Likewise, S. feltiae proved to ...
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
...nificantly more eggs and larvae on comparatively susceptible cultivars as compared to comparatively resistant ones. As comparatively resistant okra varieties viz. Bhindi Punjab Selection, Arka Anamika and Bhindi Sabazpari suffered less damage and yielded more and are therefore, recommended for cultivation to avoid damage by Helicoverpa armigera
...
Song Jiang, Fa-Lin Zhou, Jian-Hua Huang, Qi-Bin Yang* and Li-Shi Yang
...Litopenaeus vannamei larvae, different amounts of Bacillus and Lactobacillus bacteria were added to the L. vannamei aquaculture water during seeding. Seven groups were established and the microecological preparations were added every 5 d. Bacillus concentrations in the Y1, Y2 and Y3 groups were 10 ppm, 15 ppm and 20 ppm, respectively, and the Lactobacillus concentrations in the R1, R2 and R3 groups were 20 ppm, 25 ppm and...

Aqsa Khan1, Sabyan Faris Honey2*, Babar Bajwa2, Nelofer Jamil1 and Muhammad Sohail Mazhar2

...aluated for codling moth larvae. Two diets containing wheat germ in their composition as major protein source and one diet without wheat germ were used. Addition of wheat germ in the diet composition for larvae resulted in significantly high percent survival (maximum value) (60± 4.56 and 56± 2.56) and significantly increased larval body weight of codling moth (0.58± 0.04 and 0.42± 0.03 gm). While ...
Muhammad Arshad1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Yasir Iftikhar2, Samina Khalid3, Zahoor Hussain4, Jaime Molina-Ochoa5,6 and John E. Foster6
...against citrus leafminer larvae. Two laboratory bioassays, leaf dip bioassay (LDB) and topical bioassay (TB) were developed to check the efficacy of tested chemicals and essential oils. As seen from results, abamectin showed significant mortality (63.5%) of CLM larvae when topical bioassay was performed. Similarly, the percent mortality of CLM larvae was 53.8% after application of abamecti...
Fakhra Soomro1,*, Riffat Sultana2 and Muhammad Saeed Wagan2
...ssed through six instars larvae. Emergence of larval instars in both species was started from the month of July just after monsoon rainfall, whereas, S. savignyi immature emerges earlier than S. rubescens rubescens and increases their density rapidly in open grounds and rocky areas. Immature stages were found exclusively geophiles in nature and usually giving two generation in a year but occasionally third generation of diapauses eggs of S. sa...
Ailing Huang, Lingyu Meng, Wei Zhang, Junyan Liu, Guangyao Li, Huihua Tan, Wen Lu and Xialin Zheng*
...p>-6th instar larvae in Phauda flammans Walker (Lepidoptera: Zygaenidae) were tested using the leaf-dipping method. Meanwhile, effects of these pesticides on detoxifying and protective enzymes in P. flammans larvae were also measured. Results showed that the LC50 value of beta-cypermethrin, abamectin, chlorpyrifos, thiamethoxam and bisultap against P. flammans ...
Shengjie Zhou1,2, Pengfei Wang1,2, Chao Zhao1,2, Mingjun Fu3, Jian G. Qin4, Lihua Qiu1, Zhenhua Ma1, 4,* and Maoshang Lin1
...i>Trachinotus ovatus larvae was cloned in the present study. The full length of L-FABP cDNA from golden pompano was 604 bp, including a 5’-untranslated region (UTR) of 154 bp, a 3’-UTR of 69 bp and an open reading frame (ORF) of 281 bp. L-FABP encoding a polypeptide of 126 amino acids with a predicted molecular weight of 14.06 kDa and a theoretical isoelectric point of 8.73. The results of qRT-PCR showed that the highest tissue expressio...

Bina Khanzada1*, Ghulam Hussain Abro1, Tajwar Sultana Syed1 and Nazir Ahmed2 

...ellus pupae, C. flavipes larvae, C. Infuscatellus larvae, C. flavipes moths, C. flavipes adults was higher when parasitoid was fed on marigold, Holly hock and sunflower; while in 2014, the coccoon development and exist holes were higher when parasitoid was fed on marigold flower than sunflower and Holly hock; while C. Infuscatellus pupae and moths were higher in sunflower and Holly hock diets. However, control diet resulted ...
Hayat Zada1, Ahmad-Ur-Rahman Saljoqi1,*, Ijaz Ali2, Bashir Ahmad1, Abdul Waheed Khan2 and Shabeer Ahmad2
...ed from 30 overwintering larvae of each population of Matta, Madyan and Kalam regions. Out of 30 tested primers, 21 amplified 157 polymorphic bands in three populations. The mean gene frequency (f), genetic distance (I) and Shannon’s information index (h) for three populations were 1.336, 0.304 and 0.437, respectively. Nei’s unbiased measures of genetic identity and genetic distance revealed that higher genetic distance was observed among the isola...
Wang Zaigui1,*, Guo Panpan1, Ye Miao1, Sun Linghong1, Zhang Hongfu2 and Liu Chaoliang1,*
...th 1500 healthy silkworm larvae. There were two treatment groups: control group, without added Bacillus subtilis, and B.subtilis group, with B. subtilis at 2.0×1010 CFU/mL added to the feed. Experimental diets were fed in three phases: 3rd instar, 4thinstar and 5th instar. Furthermore, 3 replications were maintained and 250 silkworm larvae were reared in ea...
Lichao Feng1,2, Shaoqing Zhang4, Dianyuan Chen2, Sina Adl3 and Donghui Wu1,4*
...stribution of adults and larvae alternated between flower and corn growth stages in the field because food resources and foraging behaviors stimulated and restrained the acquisition of supplemental nutrients as a strategy for reproduction. To evaluate their food choices, wireworm were exposed to different types of plants associated with their farmland habitat, as follows: mixed pollen and nectar of flowers (Erigeron annuus, Trifolium repens, H...
Ghaleb Adwan*, Sami Bdir and Kamel Adwan
...tode cercariae. Digenean larvae were obtained from M. praemorsa snails that were collected from Palestine. Cercaria melanopsi palestinia I and III were identified as a xiphidiocercaria belonging to the microcotylae sub-group and as a microcercous cercaria, respectively. Phylogenetic analyses based on sequences of the ITS2 region of the nuclear rDNA revealed that C. melanopsi palestinia I belongs to the family Lecithodendriidae Luhe, 1901, ...
Asad Abdullah, Muhammad Irfan Ullah*, Abu Bakar Muhammad Raza, Muhammad Arshad and Muhammad Afzal
...of S. litura. The larvae of S. litura were offered leaves of cabbage, alfalfa, sesbania and, maize in comparison to artificial diet under laboratory conditions (32±05 oC; 65±05 RH). Thelarval suitability was found maximum on cabbage followed by sesbania, alfalfa and minimum on maize. The larval length and weight were found higher on cabbage followed by alfalfa and sesbania. Similarly, the pupal development was minimum in ...

Muhammad Arshad1, Muhammad Irfan Ullah1*, Muhammad Afzal1, Mian Anjum Murtaza2, Ejaz Ahraf3, Zahoor Hussain4, Syed Muhammad Ali Zahid1 and Maryam Riaz1 

...ue to the feeding of CLM larvae was observed higher 44.2% on Fairchild, 36.5% on seedless Kinnow, 36.3% on Feutrell’s early and 35.8% on Kinnow that showed great susceptibility to CLM. Smallest mines generated by CLM larvae were found on China Lemon and Succari showing the lowest damage percentage compared to other cultivars. Conclusively, the C. tangerines and C. mandarins were the most preferred cultivars by CLM
Muhammad Khalid Rafique1, Rashid Mahmood1,*, Ziyad Abdul Qadir1,Imran Bodlah2 andFarid AsifShaheen2
... queen cells vs. grafted larvae over the study interlude. The highest larval acceptance 80.85 ± 0.64 and 81.25 ± 2.05 was observed by addition of royal jelly in March and April and lowest 42.05 ± 0.69 and 48.21 ± 2.02. The shortest pre oviposition period by addition of royal jelly in March and highest was by addition of distilled water droplet during March and April. The length of queen cell was highest in royal jelly added treatmen...
S. Jalbani1, N.T. Narejo2,* and P. Khan2 
 

 

... fish (tilapia), tubifex larvae, chopped chicken viscera and dead small fish (tilapia) were used as live and prepared feed with control. It was observed that the experimental fish fed with chopped chicken viscera exhibited significantly better growth (129.10 ± 2.90 g weight gain) followed by chopped small fish (103.90 ± 1.10g) as prepared feed while live small fish resulted 3rd preference (81.89 ± 1.11 g). Significantly lower gr...
Saleh S. Alhewairini
...oratory conditions. RPW (larvae and adult) showed high susceptibility to both acetamiprid and sulfoxaflor. In addition, both insecticides had significant effect on the mortality of RPW (larvae and adult) except the pupal stage under laboratory conditions. After direct spray application of the recommended dose, the adult mortality percentages were 66.67, 73.33 and 100% for acetamiprid and 23.33, 60 and 96.67% for sulfoxaflor ...
Mubasshir Sohail1,2,*, Raza Muhammad1 and Qadeer Ahmed Soomro1
...43±2.13%) was for larvae, which fed upon Oleander aphid, Aphis nerii. Less larval mortality (7.86±2.37%), larval period (164.57±8.16 h), maximum pupal weight (9.86±0.41 mg) and emergence (95.13±2.03%) was observed when C. carnea was provided with eggs of Sitotroga cerealella. Bervicornye brassicae was performed well than A. nerii but was found least successful as compared to eggs of S. ce...
Zelle Huma1*, Ahmad-Ur-Rahman Saljoqi1 and Farman Ali2
...>Galleria mellonella larvae were reared in the laboratory. When  samples soil were collected from different regions of Khyber Pakhtunkhwa for nematodes collection G. mellonella larvae were left in sampling jar and were collected back after 2-3 days’ post application. Dead larvae were then put on the white trap and EPNs has then collected afterward. It is concluded on ...
Mustafa Koyun1*and Şenol Çelik2
...pictorum (glochidial larvae) and D. spathaceum (digenetic trematode) on the weight and sex of the host fish (Mastacembelus mastacembelus) by using some data mining algorithms. During this study, the fish weight (g) and length (mm) of 122 fish were measured. In addition, the distribution of M. heteranchorus and U. pictorum in each lamella of the gills and the distribution of D. spathaceum in the right and left eye lenses w...

Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*

... effects on brine shrimp larvae with LD50 values of 116.72 µg/ml and 168.16 µg/ml respectively. The antimicrobial and cytotoxic parameters reported can be considered as quality standards of H. gilesii in herbal industry. 

...
Jam Nazeer Ahmad1,2*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2*Mubasher Ahmad Malik1, Mujahid Manzoor1, Muhammad Tahir2,  Zubair Aslam4, Sumaira Maqsood5, Ishita Ahuja3 and Atle M. Bones3
...nd 4th instar larvae collected from three different geographical areas of Punjab (Pakistan). A difference in larval mortality, pupation, adult emergence and egg ecluson was observed. The higher but sub-lethal dose rate of NPV with Spinosad exhibited synergistic interaction, while the rest of the combinations were found additive in all the tested populations. The results confirmed the population of S. litura from Rahim Yar Khan Region least su...
Abdurakhim Kuchboev1, Mehmonjon Egamberdiev2, Rokhatoy Karimova1, Oybek Amirov1 and Mitsuhiko Asakawa3,*
.... Brachylaima sp. larvae were found in 3 (1.6%) of 95 Pseudonapaeus sogdiana. The total number of larvae per snail varied from 8 to 110 individuals. Alignment of the first four sequences of 28S rDNA was revealed a 99-100% similarity to D. dendriticum. Larvae from P. sogdiana snails were 98% similar to Brachylaima sp. In this study, it was confirmed that 2...
Babar Khan1,2 *, Nazir Javed1, Sajid Aleem Khan1, Nasir Ahmed Rajput1, Muhammad Atiq1, Abdul Jabbar1, Abdul Rehman3, Anam Moosa1 and Muhammad Amjad Ali1*
... against last instar larvae of four lepidopteran insects, wax moth (Galleria mellonella), pink bollworm(Pectinophora gossypiella), eggplant fruit borer (Leucinodes orbonalis) and armyworm (Spodoptera litura)at 27±2°C under laboratoryconditions. The results indicated that the larvae of G. mellonella and S. litura were better host as compared to P. gossypiella and...
Godwin Ikechukwu Ngwu1*, Maria Ifeyinwa Ngwu2, Fabian Chukwuemenam Okafor1, Aruh Ottah Anaga3 and Christopher Didigwu Nwani1
...n 4th instars larvae of both laboratory-reared and field-collected Aedes aegypti were carried out. The 4th instars larvae of laboratory-reared and the field-collected A. aegypti mosquitoes were exposed to 5000mg/L, 2000 mg/L, 1000 mg/L, 500 mg/L, 100 mg/L, 50 mg/L of the extract. The solvent for the extract, dimethyl sulphoxide (DMSO) was used as control. The results indicate concentration...

Hamza Rehman*, Nida Ahmad Khan, Asad Ali, Warda Batool and Kanwal Razzaq 

...mean mortality of khapra larvae on treated filter paper after 72 hours respectively. On treated wheat, thiamethoxam provided 82.61% while imidacloprid gave 78.18% mean mortality of khapra larvae at 2ppm after 72 hours. Mortality was dose and exposure dependent. Results demonstrated that thiamethoxam was more lethal as compared to imidacloprid for concern insects. 

...

Muhammad Ramzan, Unsar Naeem-Ullah*, Mahreen Hanif, Muhammad Nadeem, Mirza Abdul Qayyum, Muhammad Javaid 

... all over the world. The larvae feed on foliage and cause severe damage. Present study was carried out under laboratory conditions to elicit information about every stage like egg, larva, pupa and adult of Plutella xylostella on natural diet. The study revealed that incubation period of eggs varies from 3.2-4.3 hours. There are four larval instars of Plutella xylostella. The first, second, third and fourth instar larva survived for 3-5,3,1-4 and 2-3 days respe...
Celalettin Gözüaçik1,*, Mustafa Güllü2, Ayda Konuksal3, Reşat Değirmenci3 and Ercan Akerzurumlu3
... years, and 1567 and 862 larvae were collected in the two years, respectively. Larvae were kept inside boxes covered with nets in the laboratory at 25±1◦C and 65±5% r.h. Following pupation, adult moths and parasitoids were obtained and percentage of parasitized larvae was determined. At the end of the study, Bracon (Habrobracon) stabilis (Wesmael) (Brac...

Muhammad Farhan1, Ghulam Murtaza1, Muhammad Ramzan2, Muhammad Waqar Sabir3, Muhammad Arsalan Rafique2, Saif Ullah

...al of Chrysoperla carnea larvae on different nymphal instars of Myzus persicae was investigated in ambient laboratory conditions at University of Agriculture, Faisalabad. Chrysoperla carnea was found very active and consumed all stages of aphid. The predation rate of Chrysoperla carnea was increased with increase in larval instars (1st to 3rd). Third instar of Chrysoperla carnea was very voracious feeder and fed large number of aphid instars nymphs (1st, 2nd a...
Mehmet Mamay1* and Ayşin Bilgili2
...checked for damage, egg, larvae and pupae of CM during harvest. According to the results from the study, CM infestation rate was low in orchards where BSD infestation rate was high in pomegranate fruits. In terms of infestation rate in pomegranate fruits, a statistically strong negative relationship was determined between BSD and CM (R (BSD, CM) = -0,724;Y=46.312-0.467*X). In this study, BSD infestation rate explained the variance of CM infestation ...

 Muhammad Rafay1,*, Ghafoor Ahmad1, Tahira Ruby2, Muhammad Abdullah3, Fahad Rasheed4, Muhammad Abid1, Sohail Akhtar5, Zulfiqar Ahmad6 and Riaz Hussain7

...iding insects, flies and larvae to their young ones. Highly sensitive video camera was arranged to check their ecological behavior and breeding calls. Sentinel, breeding, roosting and bathing behaviours were studied along with vocalization. Statistical packages (mean values and standard deviation) were used to find out the potential results.
...
Sajjad Ali1,*, M. Irfan Ullah2, Asif Sajjad1, Muhammad Zeeshan Majeed2, M. Aslam Farooqi1, M. Shahid Rizwan3, Qaiser Shakeel4, Sohail Akhter1,Muhammad Raheel4 and Muhammad Arshad2
...ignificantly better when larvae were fed on gram. The relative growth rate (RGR) and relative consumption rate (RCR) values were the maximum (2.09 mg/mg/day and 10.14 mg/mg/day, respectively) in individuals fed on gram. Therefore, the efficiency of conversion of ingested food (ECI) was the highest (70.65%) in gram feeding as compared to other host plants. The areas of fore and hind wings (0.98 cm2 and 0.79 cm2) and hind tibia length (0.82...
Muhammad Usman Asif1*, Sadam Hussain Soomro2, Raza Muhammad1, Sikandar Ali Soomro2
...st 3rd instar larvae of Helicoverpa armigera under laboratory conditions at their low, recommended and high doses by leaf dip method. The results concluded that significantly high toxicity was shown after treatment with Neomed 3 SC which caused 100 % to the insect at its recommended (200 ml) and high dose (400 ml) after 24 hours of application followed by Voliam Flexi 300 SC causing 66.66 % and 75% mortality on its recommended (80 ml) and hig...

Kajol, A. H. Bhat, Aasha and A. K. Chaubey

Biochemical and molecular characterization of Photorhabdus akhurstii associated with Heterorhabditis indica from Meerut, India
...H3 against G. mellonella larvae at 48 hours was 8 IJs/larva for 50% larval mortality whereas in Helicoverpa armigera it needed 13 IJs/larva, to kill 50% of the larvae.

...

M. Rufai1† , A. A.Wahab2 , Q. O. Adeshina1 , J. C. Umehokoh1 and O. O.Ologunro1

Occurrence of entomopathogenic nematodes in Osun State, Southwestern Nigeria
... twice using last instar larvae of the Galleria mellonella (greater wax moth) for presence of entomopathogenic nematodes. Four EPN isolates were recovered viz., Heterorhabditis indica, Heterorhabditis bacteriophora, Steinernema karii and Steinernema wesieri. Of a total of 110 soil samples examined, EPNs were found in 90.90% soil samples, they belonged to the genera Steinernema and Heterorhabditis. Steinernema spp. was found prevalent in both cultivated and und...

Syeda Farzana Bibi* and Siraj-ud-Din

Fluorescence Analysis, Extractive Values and Cytotoxic Screening of Crataeva adansonii DC Leaf and Bark through Brine Shrimp Larvae Assay
...c activity. Brine shrimp larvae assay was conducted for this purpose. Extractive values of leaf and bark parts were estimated using n-hexane, chloroform, ethanol, methanol, water, and ether as a solvent. 10g of plant powder was dissolved in 200 ml solvent during various extract preparations Fluorescence study was conducted after treating the powder drug with different reagents including ethanol, methanol, acetic acid, n-hexane, HCl, nitric ...
Anum Feroz1, Abdul Rauf Shakoori2 and Farah Rauf Shakoori1*
...nd 6th instar larvae of susceptible laboratory strain (Lab-S) and deltamethrin resistant population (GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3, the activities of total esterases, cholinesterase and carboxylesterase increased significantly in both the 4th and 6th instar larvae
Chao Zhao1,2, Sigang Fana2 and Lihua Qiua2,3,*
... small;">Although shrimp larvae are susceptible to many pathogens and water quality and diseases affect commercial shrimp production, the relation between these factors are less studied. Therefore, we aimed to investigate the effect of bacterial dynamics and water quality factors on Penaeus monodon larvae. We recorded the survival rates, water quality (total ammonia, nitrite, and nitrate concentration), heterotrophic&...
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
...>C. quinquefasciatus larvae was observed by Maerua arenaria (87%), N. indicum (84%) and Withania coagulans (83%) extracts. While, the most toxic plant extracts against O. obesus termites were Periploca aphylla, Rhamnus spp. and Buxus papillosa causing 89, 62 and 52% mortality, respectively. These findings corroborate the effectiveness of indigenous plant extracts as safe and environment friendly alternates t...

Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Mushtaq Ahmad1, Muhammad Saleem2, Ali Aziz2, Irfan Rasool2, Zeshan Ali3 and Muhammad Amin2

An Update on Biology, Extent of Damage and Effective Management Strategies of Chickpea Pod Borer (Helicoverpa armigera)
...ycle passes through egg, larvae, pupae and adult stages in about 4-5 weeks. 1st to 3rd instar larvae generally feed on leaves, twigs and flowers. In later stages larger larvae (4th to 6th instar caterpillars) shift to developing pods by making holes/bores and consume entire developing seeds. Pod borers can cause yield losses up to 90 percent depending upon the insect density and susceptibi...
Maria Syafiqah Ghazali1, Azlan Che’ Amat2 and Nor Azlina Abdul Aziz1*
...mined for parasite eggs, larvae, and oocysts by simple faecal floatation and formalin – ether sedimentation technique. All large felines in the zoo were infected with gastrointestinal parasites. A total of six species of gastrointestinal parasites were recovered including four nematodes (Toxocara cati, Ancylostoma spp., Toxascaris leonina, and Oxyuris sp.), a cestode (Spirometra sp.), and a protozoan (Isospora sp...

Rahat Afza, Muhammad Asam Riaz*, Muhammad Afzal and Muhammad Zeeshan Majeed

Adverse Effect of Sublethal Concentrations of Insecticides on the Biological Parameters and Functional Response of Predatory Beetle Coccinella septempunctata (Coleoptera: Coccinellidae) of Brassica Aphid
...II response was shown by larvae of C. septempunctata in control treatment. Larval coccinellids showed type II functional response after feeding on the brassica aphids (Brevicoryne brassicae) treated with thiamethoxam, lambda-cyhalothrin and cypermethrin, while imidacloprid, profenophos and chlorpyrifos treated aphids altered the functional response of larval coccinellids from type II to III. The change in response was linked to unconsciousness and disorientati...

Sabina Noor1*, Fatimah Abang1 and Hamady Dieng2

Biology of an Exotic Butterfly Acraea terpsicore (Linnaeus, 1758) (Nymphalidae: Heliconiinae), in a Newly Invaded Region, Sarawak, Borneo
...tars. The ontogenesis of larvae was accomplished without any disruption of diapause. The female adults were ochreous orange in color with a wingspan of about 50-60 mm. The males were brighter orange in color and had a wingspan of about 40-50 mm. The population index was found to be common throughout the year but declined during months with higher precipitation. Being an exotic species, A. terpsicore is likely to keep track by feeding on its primary and famous ...
Eslam Moradi-Asl1*, Behnam Mohammadi Ghalehbin2, Kamran Akbarzadeh3, Jafar Mohammad-Shahi4, Hajar Afshin4
... myiasis occurs when fly larvae infect open wounds. We describe an old male with wound myiasis by agent of Lucilia cuprina, who had a history of diabetes and also blind that reefed to hospital in 13 September and was admitted to date 18 September 2019.Larvea extracted manually from thumb finger of left foot and sent to Medical Entomology Laboratory of the School of Public Health, Ardabil University of medical Sciences for identification. This case is a ...
Wenyou Huang1, Dan Yü1, Song Huang1,2, Jian Xiao2, Ping Qi2, Anhua Song2* and Zhen Huang1*
...>Plutella xylostella larvae as the host resulted in the isolation of a collection of mutants with increased virulence. One mutant, designated, MlUV-40b showed 3.3-fold increase in virulence as compared to the wild type parent, with an LC50 = 0.3 ×105 conidia/ml versus 1.1 × 105 conidi...
Laura Alejandra Arriola-Mosqueda1, Yadira Jiménez-Lara2, Eugenia del Carmen Prieto-Avella1, Carlos Ricardo Cruz-Vazquez3, Roberto Montesinos-Matias4, Mauricio Valencia-Posadas2, Ana Martha Cruz-Avalos5, Jaime Molina-Ochoa6,7 and César Andrés Angel-Sahagún2,*
...liae on the eggs and larvae of Rhipicephalus sanguineus, as well as the LC50 on R. sanguineus larvae, were determined and the germination (at 18 and 24 h post-inoculation) and radial growth (after 14 days) of four strains of M. anisopliae. We evaluated the pathogenicity on the eggs and larvae of all tested strains of R. sanguineus at day 3 and 7. ...
Ammara Gull-e-Fareen1,2, Imran Bodlah1,*, Muhammad Adnan Bodlah3, Muhammad Tariq Rasheed1, Habib Ali4 and Muhammad Asif5
...llaspidia attack the larvae of order Diptera and Coleoptera. Callaspidia notata (Boyer de Fonscolombe, 1832) (Hymenoptera: Figitidae: Aspicerinae) is reported with colour variations like scape, pedicel and basal part of antennal segment 3 slightly darker than remaining segments, scutellum and mesopleura mostly dark reddish with some darker areas, legs also reddish brown except tarsal segments lighter in colouration. Suspected trophic associations wi...

Syed Mazuan, Syed Mohamed, Insyirah Ishak, Dzolkhifli Omar and Norhayu Asib*

...omic threshold level (10 larvae/ frond) within 15 DAT. Both cypermethrin and MPOB Bt1 resulted in the highest mortality of bagworms which was 83%. Then, flubendiamide, chlorantraniliprole and Btk resulted in mortality rate of 82%, 75%, and 70% respectively. All aforementioned, insecticides applied were able to suppress the population up to 30 DAT.

...

Muhammad Arslan Ibrahim1*, Aneeqa Aleem2, Farkhanda Manzoor2, Shahbaz Ahmad1, Hafiz Muhammad Zahid Anwar1, Talia Aroob2 and Mansoor Ahmad3 

...at the 4th to 6th instar larvae, being more voracious, could be destructive for the maize crop if not managed properly. A large number of survivors could enhance the number of individuals in the next generation. 

...
Ahmed Saud Alsaqufi1, Sheikh Mustafizur Rahman2,3*, Roshmon Thomas Mathew2, Yousef Ahmed Alkhamis1,2, Md. Moshiur Rahman3,4 and Muhammed Aslam Pathiri2
...raits of African catfish larvae under laboratory condition. Newly hatched larvae were stocked in plastic aquaria (10L) at a rate of 5 individuals/L and reared for one month under four treatments such as 24h light (24L), 24 h dark (24D), 12h light and 12h dark with PVC (12DL_PVC), and 12h light and 12h dark without PVC (12DL) conditions. A total of 108 larvae were sampled for phenotypic tra...
Md. Alal Hossen1, Mohammad Amzad Hossain1*, A.K.M. Munzurul Hasan1,2, Bipresh Das1, Sohel Mian1, Mohammed Mahbub Iqbal1
 
...after fertilization. The larvae-initiated hatching at around 21:00 h after fertilization and total length of larvae were computed 1.11±0.01 mm while the temperature of water was 30±1°C. The present study revealed that application of S-GnRHa hormone at dose of 0.5 ml kg-1 body weight of female could be efficacious for production of M. gulio seed in freshwater successfully.
 
Jen-Hong Chu1, Ya-Wen Lan2, Shyn-Shin Sheen3 and Angela Chien3*
...ps of L. canarium larvae. The final weight, weight gain percentage and specific growth rate of L. canarium larvae fed with diets L6 and L12 were significantly higher than those of larvae fed with diets L0, L2 and L4. The feed conversion ratio (FCR) of L. canarium larvae fed with treatment diets was significantly lower than that of co...
Muhammad Ramzan1*, Unsar Naeem-Ullah2, Muhammad Umair Sial2, Naeem Iqbal2 and Shafqat Saeed2
...n various countries. The larvae of the pest can cause 80-100% defoliation. The various confirmed host of T. varians include Ficus benjamina, F. religiosa, F. benghalensis, F. caraica, F. infectoria, F. elastica, F. nitida, Artocarpus communis, A. heterpphyllus and A. kamansi. Fairly limited studies are documented for its chemical and biological management. For chemical control, deltamethrin was proved more toxic while among biological control agents Goryphus s...

AdlyAbd-Alla1,2, MahaNada3, Francois Cousserans1, and Max Bergoin1

...spension to third instar larvae of Galleria
melonella and Spodopteralittoralis failed to induce any mortality indicating that the virus is
most likely host specific to N. viridula. This virus is similar to the previously reported NVV-1
virus in N. viridula from South Africa strongly suggesting that NVV-1 is widely distributed in
Africa and might play an important role in regulating the insect population in field ...
Hassan Shahzad Khan1, Muhammad Tariq1*, Tariq Mukhtar2 and Asim Gulzar1
...of 3rd instar larvae of A. albopictus was caused by datura extract followed by neem and clove. On the other hand, neem and clove extracts caused the maximum mortality (80%) of 4th instar larvae followed by datura extract (78%). Similarly, the minimum mortalities of 3rd and 4th instar larvae were caused by bitter gourd extract. In case of g...
Nabila Kousar1, Tanzeela Riaz2, Anum Feroz1, Abdul Rauf Shakoori3 and Farah Rauf Shakoori1*
...st 4th instar larvae of GUW, DGK, OKR, Layyah and LAB-S were 514, 353, 303, 189 and 98 ppm respectively while for the DE these values were 3606, 2684, 2407, 2192 and 2065 ppm, respectively. Based on the values of LC50 deltamethrin was found to be more effective than DE. Among the tested populations, GUW population was most resistant while LAB-S population was least resistant. Efficacy of deltamethrin and DE in various combinations was als...

Moheem Khan1, Arif Ali1*, Shafique Ahmed Memon1, Taimoor Khan Qambrani2, Ghulam Khaliq3, Jahanzaib Khan1, Saghir Ahmed1, Azmat Hussain Abro4 and Sohail Azeem Baloch1

...ty was observed in eggs, larvae and pupal stage at all given timings of UV-B light compared with control. Incubation period of eggs, developmental period of larvae and pupae gradually increased as increasing exposure time of UV-B light. Statistically, no significant difference was observed between 5 minutes UV-light and control group on the incubation period of eggs and larval stages. Significantly, adult longevity of T. cas...
Tanzeela Riaz1, Farah Rauf Shakoori2*, Syed Shahid Ali2 and Mushtaq Ahmad Saleem1
...gnificantly increased in larvae of both populations throughout the phosphine exposure period with reference to their respective unexposed group values. In case of soluble proteins, an elevation was observed which was followed by a decline after 72 h. Similarly, free amino acid contents showed increasing trend in both larval instars of tested populations throughout the exposure period with the exception of the 4th larval instars of susceptible popula...
Tanmoy Mandal1 and Sunil Kr. Ghosh2*
...ighest population (20.42 larvae/5 leaves) recorded on 32nd standard week that is on the 2nd week of August. Leaf miner population had a significant positive correlation with temperature and relative humidity. A mixed formulation of imidacloprid with azadiraction was found to be the most effective against leaf miner showing 79.24% mortality with imidacloprid 76.25% and in the mixed formulation imidacloprid+polygonum 73.74 %. It is conclude...
Funda Turan1*, Ihsan Akyurt2 and Sehriban Cek-Yalniz1
... on C. gariepinus larvae for 30 days, and the effects of red clover extract on sex reversal and gonadal development were also examined at the end of 120 days.At the end of experiment, highest feminization (89%) was observed at 50 mg L-1 red clover group. Morphological and histological examinations of the gonads in all groups revealed no intersex fish. Histological examination of fish treated with red clover extract revealed no damage to the t...
Muhammad Rizwan1*, Bilal Atta1, Misbah Rizwan2, Arshed Makhdoom Sabir1, Muhammad Tahir3, Muhammad Sabar1, Muddassar Ali1 and Muhammad Yasir Ali4
...ed survival rate of both larvae and pupae. Si amendments reduced larval and pupae weight as compared to control treatment. However, the consumption of green matter increased in Si treated plants by 3rd instar larvae of C. medinalis. Results also showed that enhanced Si level in rice plants reduces food quality and increases plant resistance to the C. medinalis in rice variety. This study concluded th...
Ardi Prasetio1*, Christina Maria Sri Lestari2, Sutaryo Sutaryo2, Manar Fayiz Mousa Atoum3,4Muhammad Zahoor5, Asma Nisar6 and Muhannad Illayan Massadeh7

Abbas M. Al-Azab1*, Ahmed A. Zaituon2, Khalid M. Al-Ghamdi3, Fahd M. Abd Al Galil4,5 

... immature stages, 83.41% larvae and 16.6% were pupae. The obtained statistical analysis showed that the availability of the adult and juvenile stages of A. aegypti occurred throughout the year in different densities depending on the climatic conditions and the effectiveness of the breeding sites. The density of adult were 2.61 (Ghuleel), 2.55 (Al-Jameiah), 2.25 (Al-Balad), 2.47 (Al-Safa) and 1.86 (Al-Nazlah Al -Yamaneyyah). Whereas the density of immature stag...

Li Wei, Wei-wei Shao and Zhi-hua Lin*

...rvival and growth of the larvae of two anuran species, Fejervarya limnocharis and Microhyla fissipes. Our results showed that survival rates under most pesticide treatments (22/28) and growth under all pesticide treatments were lower than those under the control treatment. Mortality and growth reduction rates under treatment with pairwise combinations of pesticides were rarely higher than those under treatment with individual pesticides. At concentrations of 1...

Mirza Gul1*, Muhammad Zahid1, Ikram Ilahi2, Hazrat Ali2*, Fida Hussain3 and Muhammad Anwar Sajad3

...nisomeles indica against larvae, pupae and adults of Culex quinquefasciatus. The study also evaluated the predatory effects of the diving beetle, Agabus cybister, against various instar larvae of Cx. quinquefasciatus. Bioassay of whole-plant extracts was performed following WHO methods, with slight modifications. LC50 values for A. scoparia and A. indica against early fourth instar larvae ...
Abdul Jabbar1, Muhammad Tariq1*, Asim Gulzar1, Tariq Mukhtar2 and Tayyaba Zainab3
...ainst 3rd and 4th instar larvae of C. pipiens. The green synthesized AgNPs of these plants were characterized by UV-Multiskaner. LC20 and LC50 values were calculated through Probit and Logit analysis (POLO) software. All the plant extracts and their green synthesized AgNPs caused the maximum mortalities of 3rd and 4th larval instars of C. pipiens after 96 hours. With the increase in time intervals and concentrations, the mortalities increased showing direct po...

Ramy S. Yehia1,3, Essam A. Shaalan2* and Hashem M. Al-Sheikh1 

...luated against house fly larvae under laboratory conditions. Batches of house fly larvae (25 individuals per replicate and 4 replicates per dose of fungi) were subjected to five doses from each fungus (101, 103, 105, 107 and 109 conidia/ml) in plastic cups for one min then transferred to a clean one. Both test and control cups were incubated for 7 days to determine LC50, 90 and EI50, 90. Joint action of these fungi with thre...

Hassan Ali1, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1* and Muhammad Imran Hamid2

...tions against 5th instar larvae of T. granarium. Toxicity bioassays revealed that the extracts of Citrus reticulata L. and Solanum nigrum L. were most effective against T. granarium causing significantly higher larval mortality (35 – 40%) than other botanical treatments. Similarly, the highest concentration (15%) of C. reticulata extract exhibited maximum repellency (88%) of larvae, followed by Datura stramonium (84%) ...
Saeed Akram Khan1, Shahid Sherzada1*, Muhammad Ashraf1, Durr-e-Shehwar1, Sonia Iqbal1, Usman Atique1,3, Farzana Abbas1, Sohail Ahmad2 and Muhammad Sultan Haider4
...turation of all eggs and larvae that resulted in reduced hatching rate. Microscopic examination revealed that eggs were more influenced by high temperature at early stages of development. Impacts of slight change in water temperature were observed from Blastodisc to morula stage. After gastrula stage, temperature effects were evident but not critical. This was a first documented report on effects of temperature on breeding performance and embryonic development...

Momotaz Khanom*, Sheikh Mustafizur Rahman, Muhammad Yousuf Ali, Md. Shahin Parvez, Al-Hasan Antu, Md. Nazmul Ahsan 

... mass production of fish larvae has recently drawn a great attention due to inadequate supply and inconsistent price. The present study evaluated the effects of early introduction of formulated feed as a substitute for live feed on growth performances of Asian stinging catfish, Heteropneustes fossilis larvae reared in laboratory conditions. Three-day-old larvae hatched through induced bree...

Mujahid Tanvir1, Muhammad Asam Riaz1*, Muhammad Zeeshan Majeed1, Mazhar Iqbal Zafar2, Muhammad Tariq3 and Muhammad Bilal Tayyab1

...inst C. quinquefasciatus larvae. Among the n-hexane extracts of 40 indigenous plant species collected from Soon Valley and surrounding salt range of Pakistan bioassayed against C. quinquefasciatus larvae, eighteen botanicals exhibited more than 50% larval mortality in 48 h exposure. The most effective botanical extracts were Maerua arenaria Forsk, Nerium indicum Mill., Withania coagulans Dunal, Suaeda fruticosa (L.) Delile, ...

Weam Mohamed Baher1, Wageh Sobhy Darwish2*, Abdelazim Elsayed Elhelaly3,4 

...valence rate of Anisakis larvae in two marine water fish commonly consumed in Egypt, namely, herrings and sardine. Samples were collected equally from Suez, Ismailia, Damietta, Port Said and Alexandria. The obtained results revealed overall incidence rates of Anisakis larvae in herrings and sardine at 70% and 50%, respectively. The parasite infested mainly (100%) the visceral organs of the positive samples in the two fish sp...

Ghulam Murtaza1,6*, Muhammad Irfan Fareed2, Noshaba Munawar3, Muhammad Nadeem Khan4, Gul-E- Zahra5, Taskeen Ahmad6, Muhammad Daud6 and Taqi Raza6

...more toxic to A. aegypti larvae as compared to matrine, chlorfenapyr and imidacloprid after 72-h exposure. While minimum mortality was recorded after 24-h exposure of insecticides in all treatments. So, it can be concluded that spinosad was more toxic to kill A. aegypti larvae.

...

Mahreen Hanif*, Shafqat Saeed, Mudssar Ali, Muqadas Younas, Huda Bilal, Syeda Fatima Bukhari

...ulation of T. castaneum (larvae and adult) as well as greater weight loss (%) in chickpea was recorded in polypropylene (green) packet in contrast to polyethylene (gauge 2) and china lamination. Significant differences were observed in the overall adult population of T. castaneum (p<0.05) and chickpea percentage weight loss (p<0.05) than its larval population (p>0.05) during the storage period. However, more adult population and weight loss (%) occurr...
Faiza Saleem1, Aly Khan2*, Rafia Rehana Ghazi3, Nasira Khatoon1, Samina Waheed1, Mian Sayed Khan4
...ected with Raphidascaris larvae. On the average 40 to 48 percent of the larvae found were alive. Five thousand worms in just 4 fish can be very harmful and may lead to further decline in the population of host fish.

...

Nimra Altaf1, Muhammad Arshad1, Muhammad Zeeshan Majeed1, Muhammad Irfan Ullah1*, Hamza Latif1, Muhammad Zeeshan1, Gulfam Yousuf1 and Muhammad Afzal2

...and 100 ppm) against FAW larvae in maize. Our findings showed a significant effect (P < 0.001) of these chemicals on the mortality and consumption rate of FAW larvae. Results show that mortality increased in all treatments with time. Recommended dose of chlorantraniliprole (50 ml/100 litre water) insecticide showed 71.0% mortality of FAW on 5th day, which increased up to 82.0% on the 7th day after application of treatment...

Rahmat Ullah Khan1*, Asif Sadam2, Karim Gabol1, Waheed Ali Panhwar3, Sajid Mahmood4, Mustafa Kamal5, Hamid Ullah6, Syed Abidullah7, Muhammad Tufail8, Bashir Ahmad4, Gul Bacha Khan4 and Habib Ul Hassan1

...ey were fed totally with larvae of arthropods, most1y Lepidoptera and Coleoptera (40%), Hymenoptera (12%) and Diptera (8%). However, adults and grains were not fed. Nestlings (4th day age) were fed by adults (32%), larvae (69%), and grains (2%). While the chicks were fed mostly by adults (72%), with larvae (23%) and grains of seed (5%). Significant differences were present in the growth of...

Muhammad Furqan and Zulfiqar Ali

...including ants, insects, larvae, and grit were also recorded. According to respondents the highest sighting (62.4%) of kalij pheasant was recorded from the forest, followed by cultivated land (20.4%). Major threats to kalij pheasant include forest fire (41.6%), followed by hunting (27.2%), habitat destruction (18.8%), and natural predators (12.4%). The hunting (n=142) of kalij and hunting index (0.855) was recorded during the study period. The maximum hunting ...

Al-Hassan M. Mostafa1*, Gehan Mohammed Sayed2 

...e with 1750 of 3rd stage larvae of H. contortus (L3) and subsequently subdivided into positive infected untreated group (second group), positive infected and orally treated group with garlic juice, 5ml/ animal, (third group) and positive infected and orally treated group with coriander extract, 0.9g/ Kg b.w. (fourth group). Fecal samples were collected weekly after 3 weeks post infection for Fecal Egg Count (FEC). One animal of each group was slaughtered at 7t...

Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti 

... black soldier fly (BSF) larvae on different substrates as probiotic candidates. The LAB from the digestive tract of BSF larvae in chicken manure and palm kernel meal substrates was isolated and taxonomically identified using the 16S rRNA sequence homology and molecular identification. The LAB isolates were also tested for antimicrobial and hemolysis activities. Five isolates from BSF larvae

Sehrish Akram1, Abida Butt1* and Shakil Ahmad Khan2

... more toxic for silkworm larvae compared to lead. The acute toxicity of imidacloprid formulation (IMF), imidacloprid technical grade (IMT) and Pb showed positive correlation with the concentration and negative with the age of larvae. From second to fifth instars, LC50 values of IMF ranges from 0.72 to 2.96, IMT from 0.86 to 2.75 and Pb from 1.93 to 6.88 mg/L. The toxicity of binary mixture of IMF + Pb showed synergism agains...

Ahmad-ur-Rahman Saljoqi, Muhammad Tayeb and Muhammad Salim*

...opulation of syrphid fly larvae (0.84 plant-1), and Pukhtunkhwa-15 had maximum lacewings larvae (0.81 plant-1). Overall results revealed that PS-13 showed relatively greater resistance to aphid population with maximum wheat yield (3412.5 kg ha-1) as compared to other wheat cultivars.

...

Yasemin Yıldırım Meşepınar and İlker Kepenekci*

...try (Turkey) against the larvae and pupae of P. operculella in greenhouse-pot experiments. S. feltiae was the most effective killing 6.33±0.61 (63.30%) larvae, whereas H. bacteriophora only killed 3.67±0.56 (36.70%) dead larvae. 0.17±0.17 (1.70%) larvae died in control group after 10 days and 9.33±0.33 (93.30%) developed into ...

Naila Shahzadi1, Hafiz Muhammad Tahir1*, Azizullah1, Muhamamd Farooq Bhatti2, Shaukat Ali1, Muhamamd Summer1 and Farman Ahmad Chaudhary3

...igher in G2 (5% sericin) larvae compared to the control (G1). Similar trend was observed in G3 (3% honey) for cocoon width (1.67 ± 0.03cm), cocoon length (2.98 ± 0.05), filament length (1310.89 ± 15.58m) and percentage of fibroin (85.57 ± 0.28) (with less percentage of sericin (18.61 ± 0.28%). Furthermore, lowest (finer) denier (1.82 ± 0.07) was recorded in G2 larval group fed with 5% sericin. It is concluded from this...

Sumaria Maqsood1*, Muhammad Ali 2, Amna Shoaib3, Shahbaz Ahmad2 and Noor-ul-Ain2

...duction in the pupal and larvae mortality rate as compared to the tomato paste diet and chickpea diet. The rearing expenses for 200 larvae on the modified diet (674/Rs) were 2.5 folds less than the expenses for rearing the same number of larvae on chickpea based diet (1749/Rs.) and almost equal to the tomato paste-based diet (669/Rs.). Therefore, a modified diet based on wheat germ and tom...

Abdurakhim E. Kuchboev1*, Mehmonjon Kh. Egamberdiyev2 

...ere found to be positive larvae of protostrongylids. Based on the nucleotide sequence data, three species of nematode larvae Protostrongylus rufescens, Muellerius capillaris, and Cystocaulus ocreatus were identified. According to the morphological characteristics of the studied land mollusсs, the shell structure was identified as six different morphotypes. After molecular genetics and morphological analyzes, these land moll...

S. Feroza1, A. G. Arijo1†, F. M. Bilqees2 and M. S. Phulan1

...y the migrating A. galli larvae. Except on the top, central veins were not obvious. Inflammatory tracts produced by migrating larvae were prominent. Large vein with portal sluggish consisted of fibrinous material and inflammatory cells, disarrangement of hepatici and shrinkage of hepatocytes were found to have resulted into dilation of sinusoids.

...

A. M. Rahoo1, 2, †, T. Mukhtar3, B. A. Bughio4, S. R. Gowen1 and R. K. Rahoo5

A. M. Rahoo1,2,†, T. Mukhtar3, S. R. Gowen2, B. Pembroke2 and M. A. Rahu4

...the Galleria
larvae at infection and the numbers of IJ recovered in the moist as compared with dry conditions.
...

A. Sattar1, †, A. Khan2, N. Khatoon1 and A. Mujahid3

...caris acus (Bloch, 1779) larvae, Metabronema magnum (Taylor,
1925), Haplonema immutatum (Ward et Magath, 1917) and Hedruris bryttosi (Yamaguti, 1935) were recorded from
the gut of the catfish, Arius arius (Hamilton, 1822). These are new host records as these parasites have not been
reported from Arius arius species in the region, off the Karachi coast.
...

Belema Robert1, Favour Welenya1, Deborah Achi1, Cynthia Onyeagwara1, Soala Obie Minimah2, Ebele Anulika Obichi2, Chidinma Charity Amuzie1* 

...cted with S. stercoralis larvae. It is hence recommended that special care be taken in the cleaning and washing of this vegetable, possibly with saline water, to limit the chances of human infection.

Keywords | Strongyloidiasis, Geo-helminths, Edible vegetables, Immunocompromised. 

...

Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4

...say against brine shrimp larvae where chloroform extract showed minimal cytotoxic level (23.37%) while acetone extract showed maximum cytotoxicity (65%).

...

Hina Safdar1,2*, Nazir Javed2, Sajid Aleem Khan2, Muhammad Zeeshan Majeed3, Arif Mehmood3 and Muhammad Arshad4

... spp. against 2nd instar larvae followed by 52.80, 46.20 and 46.20% mortality of 5th, 3rd and 4th instar larvae, respectively. In case of Photorhabdus spp., maximum mortality (26.40%) was observed against 2nd instar larvae. In other bioassay, 40% suspensions of bacterial metabolites were evaluated against different larval instars of S. litura and were found significantly effective against ...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel4, Abdelwahab M. Abdelwahab1,5
...els of black soldier fly larvae Hermetia illucens (BSFL) meal on egg production, egg quality, physiological aspects and economic efficiency of laying hens. The study employed 270 commercial layers, 40-wk-old, that belonged to the W-36 Hy-Line chickens. The layers were randomly designated into five equal treatment groups (9 replicates × 6 hens per replicate for each treatment) according to the dietary inclusion levels of BSFL meal. The first group of bird...

Muhammad Shakil Ahmad1, Muhammad Afzal1, Liu Yu Feng2, Muhammad Shahroaz Khan1 and Muhammad Zeeshan Majeed1*

...tions against 3rd instar larvae of S. litura under laboratory and then under the field conditions. In both trials, insecticidal treatments affected significantly the mean mortality or reduction of S. litura larvae recorded both at two and five days post-treatment. In laboratory bioassay, combinations of flubendiamide and A. indica, flubendiamide and N. tabacum and of spinetoram and A. indica formulations caused significantly...

Anatoly Murashevich Bittirov1*, Sadrutdin Shamshitovich Kabardiev1, Ayub Yusupovich Aliev1, Boris Kazievich Laipanov2, Svetlana Aleksandrovna Shemyakova2, Ismail Anatolyevich Bittirov1  

... Echinococcus granulosus larvae, which were recorded with different occurrence values, indexes. Of these, 2 species in the region (Alveococcus multilocularis, Echinococcus granulosus) have epidemiological and epizootological significance. Indices of occurrence and abundance of cestode Raillietina sp. amounted to 6.22% and 7.1±0.6 ind./individual, respectively; species Rodentotaenia bondarevae - 3.11% and 3.9±0.4 ind./individual; species Mathevota...

J. Salma and F. Shahina

...b(255, 255, 255);"> larvae and in vitro soya flour medium were also exposed to four different temperatures. Maximum production of all other species was found at 32 ± 2 ÌŠC, except S. feltiae
Ferzana Bhatti1, Fiaz Hussain2, Zain ul Abdin1*, Muhammad Arshad1, Saqi Kosar Abbas2 and Muhammad Zeeshan Shabbir3
... healthy and mature host larvae. To observe the effects of venom injection on development of parasitoid progeny, freshly laid eggs of the parasitoid were transferred from stung hosts onto non-stung hosts. Venom caused 100% mortality of the host even in diluted forms; Dufour’s gland secretion did not cause any significant mortality. The secretions injected by an ovipositing female not only contributed to a reduction in egg mortality but also positively af...

Riaz Hussain1*, Ata-ul-Mohsin1, M. Farooq Nasir1, Zahid Akram2, Muhammad Sajid Qureshi1 and Abdul Mannan Hamzah1

...second and third instars larvae were released on cabbage varieties. Parasitism efficacy was lower on third instars larvae as compared to the second. Higher parasitism (53.53%) was assessed on Asha while lower parasitism (44.95%) was observed on Golden acre when second instars larvae were exposed. Parasitism was higher (61.17%) on Asha while lower (50.1%) on golden acre when third instars <...

Muhammad Ahsan Riaz1, Ayesha Riaz2, Muhammad Asif Mahmood3, Muhammad Uzair Mukhtar3*, Zaib Un Nisa1, Beenish Ijaz4 and Muhammad Shahid Rasool5

...riation of Aedes aegypti larvae with the changing seasonal temperature is important. The study was conducted to observe the difference in body size and different body structures of Ae. aegypti larvae in two seasons, i.e., southwest monsoon (June through September) and retreating monsoon (October and November). The fourth instar larvae were collected from areas of district Lahore. The colle...
Muhammad Rizwan1*, Bilal Atta1,  Muhammad Arshad2, Sohail Akhter3
Muhammad Tahir3, Misbah Rizwan1, Muddassir Ali1 and Arshad Makhdoon Sabir1
...lopmental life stages of larvae of ladybird beetle but it significantly reduced the span of total adult longevity (30.8 to 26.91 d), female adult longevity (31.5 to 27.79 d), and male adult longevity (29.4 to 25.56 d) as compared to control. Among population parameters, λ cyhalothin had a non-significant impact on adult preoviposition (APOP), total preoviposition period (TPOP), oviposition period, intrinsic rate of increase, finite rate of increase, and...

Muhammad Saad Waqas, Li Xia, Tian-Ci Yi*, Liang-Yu Sun, Rong Xiao and Dao-Chao Jin*

...s and adults. Nymphs and larvae relocated nest when there was abundance of exuviae whereas adults did not relocate. Adults could extend their original nest when it was filled with exuviae. Alive individuals lived with dead individuals and they did not remove the dead ones from nests. These findings highlight the hygienic challenges, adaptation of social living organisms, and demonstrated how sanitary behaviour can result from a combination of evolutionary hist...

Md. Rashedul Islam1, Abul Farah Md. Hasanuzzaman1*, Md. Latiful Islam2, Ghausiatur Reza Banu1 

...e of increasing mud crab larvae production in captivity. But some chitinolytic bacteria (Vibrio spp., Aeromonas spp., and Pseudomonas spp.) poses major threats in the production line of mud crab larvae. Hence, the present study tried to determine prevalence and load of total and pathogenic bacteria in the different components of the mud crab hatchery. Selective agar media, a series of biochemical tests, and bacteria isolatio...

Ahmad-Ur-Rahman Saljoqi1, Imtiaz Khan1*, Ijaz Ahmad1, Javed Khan2, Shahid Sattar1, Bashir Ahmad Khan and  Muhammad Salim

...parameters indicate that larvae duration was 10.34 days. The larvae consumption rates were 379.2 aphids during their life span. Functional response of larval instars indicates that with increasing prey densities, the number of aphids consumed linearly increase and then become static after attain the asymptote level at certain densities were 25, 35 and 70 aphids per first, second and third instar larv...

Fuji Astuty Auza1*, Sri Purwanti2, Jasmal A. Syamsu2, Asmuddin Natsir2, Rusli Badaruddin1, Deki Zulkarnain1, La Ode Muh. Munadi1 

... black soldier fly (BSF) larvae flour to reduce the use of fish flour and improve performance, carcass quality, and nutrient digestibility of village chicken. A total of 140 one-day-old chicks (DOC) were randomly assigned to one of five treatments in a completely randomized design, with four replicates per treatment. The treatments included P0 = 0% BSF larvae flour, P1 = 25% (3.74% BSF larvae<...

Ayoub Hadjeb1,2*, Ismahane Lebbouz3 and Yasmine Adjami4

...e (RGR) were observed in larvae fed by Deglet Nour variety just as The efficiency of biomass conversion of digested food (ECD) and ingested food conversion efficiency (ICE) in Ghars and approximate digestibility (AD) in Mech Degla. The calculation of gravimetric index (nutritional) consumption and utilization of food showed a very highly significant difference (RRI,RGR, ECD and ICE: P <0.0001; AD: P <0.0004) between larvae...

Li Qiao*, Shubao Geng, Shibao Guo, Hongmin Liu, Jian Yin and Quan Zhang

..., the 2nd and 5th instar larvae were irradiated under light at the wavelengths of 400 and 420 nm with 6 different irradiation times (0, 60, 90, 120, 150 and 180 min). The development duration, pupation rate, abnormal pupation rate, pupal weight, adult emergence rate, male and female adult longevity, oviposition rate of S. subpunctaria were measured. The results showed that with the increase of treatment time, the duration of 2nd instar

Muhammad Ali1*, Rukhsana Perveen2, Khalil Ahmed3, Ghulam Raza1, Muhammad Akbar1, Maisoor Ahmed Nafees3 and Muhammad Ayub4

...th adults and 4th instar larvae of this coccinellid predator in the biological control of I. aegyptiaca comparatively in the laboratory and under field conditions. The 4th instar larvae were found to be more voracious than adults.

...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
...nst Anopheles 3rd instar larvae. These findings indicated that actinobacterial isolates possess antibacterial and larvicidal potential. Further extraction and purification of bioactive components from these bacterial may be a good source of novel antibiotics and natural-insecticides.

...

Tasleem Akhtar1*, Muhammad Farooq Nasir2, Imran Bodlah2 and Muhammad Adnan Bodlah3

... cycle of A. smithi from larvae to adult was completed in about 11 days. The pre-mating period of males (n=10) varied between 5 and 8 min (mean: 4 min). Copulation time (n = 10 pairs) was between 33 and 55 sec (mean: 45.2 sec). Oviposition time (n = 10 females) was between 1 and 2 sec (mean: 1.5 sec). Female parasitoids lived longer (5.75 days) than male parasitoids (4 days) when offered honey and water as a food diet. Lifespan of adult male and females was sh...

Yuyu Wang1,2, Mingzhu Li 3,4, Gang Lin4, Xiaohua Guo5, Aihua Sun5, Hao Dong5, Qinghui Ai1,* and Kangsen Mai1,*

...Scophthalmus maximus L.) larvae. Four isonitrogenous and isolipidic diets were formulated to contain 0 (S0, control diet), 50 (S5), 100 (S10) and 150 (S15) g kg−1 Schizochytrium meal. Fish (initial body weight, 0.06 g) were randomly allotted to 12 square fiberglass tanks. Fish were fed 5 times daily (7:00, 10:00, 14:00, 17:00 and 21:00) for 28 days. No significant differences were observed in survival and intestinal morphology among fish fed various leve...

Bdour Muhammed Al-Shweily1*, Jawad Bulbul Al-Zaidawi2, Muhammed Jubair Hanawi1 

...n controlling the insect larvae and pupae. The integration of these indigenous biological control agents yielded notable mortality rates for the targeted larvae and pupae. The highest rates were achieved when using a higher concentration of fungi, specifically 20×10^6 spore/mL, in combination with 100 infective juveniles/mL of nematodes (resulting in an 83.33% mortality rate for larvae

Syed Shakeel Shah1, Ayesha Jameel1, Sabila Afzal1*, Muhammad Zubair2, Iram Fatima Bokhari1

...arasites eggs, cysts and larvae. A high prevalence of about 47.58% was described in this study. Coriander was the highest contaminated vegetable (51.42%), followed by mint (48.57%). The carrot was the minimum contaminated vegetable with 40% contamination. Examination of vegetables revealed 12 genera of parasites along with aquatic mites (5.74%). Taenia was the most prevalent parasite (25.28%) followed by Ascaris (21.83%), Toxocara (9.19%) and Hymenolepis (9.19...

Muhammad Salman1*, Naveed Ahmed2, Mir Manzar Ud Din3, Fazli Amin4 and Arsalan Ali5

...ised of thirty number of larvae and cocoons of Chinese C-21 strain. The parameters that were studied in the present experiment and recorded were weight of full-grown larvae (1.4-3.6 gm), length of larvae (49-67 mm), cocoon weight with floss (0.34-2.3 gm), cocoon weight without floss (0.32-1.76 gm), cocoon shell weight (0.16-0.83 gm), cocoon diameter (14-22 mm), cocoon length (27-34 mm), co...

Muhammad Farooq Bhatti1, Hafiz Muhammad Tahir1*, Aamir Ali1, Hooria Ashraf Khan1, Rabia Fajar Ali2, Ayesha Muzamil1, Fariha Munir3, Fatima Ijaz1, Rizwan Khurshid3

... the 5th instar silkworm larvae were assessed for biological and economic traits. The results of this study exhibited that Chinese silkworm eggs showed better hatchability at high temperatures. The larvae of the Chinese race also showed an increased leaf consumption rate with an increase in the number of meals (6) per day. Moreover, a significant increase in biological traits including average larval weight (0.979±0.0...

Arslan Muhammad Ali Khan1, Rao Zahid Abbas1*, Zia ud Din Sindhu1, Muhammad Shahid Mahmood2

...dent manner of adult and larvae of Hyalomma spp. Egg number, egg mass, and larval hatching were also reduced significantly (p<0.05) in a dose-dependent manner. As a result, reproductive index (RI), reproductive efficiency index (REI), and nutrient index (NI) were also decreased significantly (p<0.05) in a dose-dependent manner. Similarly, A. subulatum essential oil showed dose-dependent response against repellency of Hyalomma spp. The LC50 and LC90, RC50...

Agha Mushtaque Ahmed1*, Ali Zachi Abdul Qadeer Alhilifi2, Fahad Nazir Khoso1, Muhammad Ibrahim Kubar1, Tehniyat Naz Shah3 and Touseef Ahmed1

...rtificial diets. The FAW larvae were reared on two distinct composed artificial diets (bean D1 and chickpea flour D2) and one natural diet D3 (fresh and healthy maize leaves). In both artificial diets, only the flours were different, while the rest of the ingredients remained the same. Sixty first instar larvae were introduced in a Petri dish to observe larval duration, larval weight, and adult fecundity. Each larva was offe...
Hina Mumtaz, Muhammad Zeeshan Majeed*, Muhammad Afzal, Muhammad Arshad, Arif Mehmood and Muhammad Qasim
...to control S. frugiperda larvae in the laboratory as well as under semi-field and field conditions. All insecticides performed well in controlling S. frugiperda larvae under laboratory conditions. Though, chlorantraniliprole @ 50 mL/100L (58.0–100%), abamectin @ 400 mL/100L (56.0–100%), lambda-cyhalothrin @ 250 mL/100L (52.0–100%) and chlorpyrifos @ 1000 mL/100L (52.0–98.0%) showed significantly (P &l...

Muhammad Ali Raza1*, Aneela Zameer Durrani2, Muhammad Muddassir Ali3, Tariq Usman4, Bilques Bano5, Nazia Rubab6, Syed Tasadak Mehdi7, Muhammad Wasim Iqbal8, Kumayl Hassan Akhtar9, Hira Hameed10

...odes of cattle (eggs and larvae along with identification) pertaining to Public (One) Health has categorically been studied for the first time in Lahore with a proper treatment protocol and comparison with levamisole, oxyclozanide and the combination of both drugs.
...

Rudhy Gustiano1*, Iskandariah Iskandariah2, M.H. Fariduddin Ath-Thar1, Gleni H. Huwoyon4 and Deni Radona3

...lized eggs, embryos, and larvae showed no differences between observed populations. The survival rate of larvae for East Java, West Kalimantan and Lampung were 92%, 86% and 82%, respectively.

...

Ashfaq Hussain1, Javed Khan2, Ammara Blouch3, Ahmad-Ur-Rahman Saljoqi1, Ashraf Khan4,5* and Zaheer Sikandar6

...(479.6) were consumed by larvae while the minimum mean number of aphids (36.8) were consumed by 1st larva instar. Female beetle was recorded with a maximum mean number of aphids (2561) consumption as compared to male beetles (1921). Furthermore, the apparent mortality was highest at egg stage, making it the most vulnerable stage in the beetle’s life cycle, followed by 1st instar larval stage, which also showed higher mortality. The maximum survival fract...

Milena Vlahovic*, Dragana Matic, Marija Mrdakovic, Larisa Ilijin, Anja Grcic, Aleksandra Filipovic, Jelica Lazarevic and Vesna Peric-Mataruga

...onditions. Fourth-instar larvae were exposed to acute and chronic effects of two cadmium concentrations, 10 and 30 μg Cd/g dry food, as well as a three-day recovery from chronic treatments. PMM is also an indirect indicator of food consumption and was found to be significantly reduced compared to control in both acute effects and chronic treatment at 30 μg and its three-day recovery. The PMM reduction during acute treatments is a consequence of cadmium a...
Mohamedl , Shimaa D.; Ghareeb2, A.; El-Dougdoug , K. A. and Bekheitl , H.K.
...rograph of PoGV infected larvae figured out several histological changes. It was found that the midgut epithelial cells were the main targets of the virus infection. Columnar cells, goblet cells and regenerated cells loose their identity and become disorganized, also breaking down of peritrophic membrane and basement membrane and red colour appeared due to presence of granules particles. The morphological structures of P. operculella infected

El Sayed l , Yasmein A.; Ghareeb2 , A.; El-Dougdoug3, K. A. and Bekheetl , H. K.

... symptoms of slow motion larvae, cuticle showing red color and swilling of the body. Examination of BAW infected extract by light microscope showed polyhedral bodies with concentration (2.3x107PIB/ml). In our study the morphological structures of S.exigua infected with SeNPV (EG) as well as healthy one were examined by SEM. It was showed complete destruction of external larval body, reduction in each abdominal segment size, pro-legs and thoracic legs reduce in...

Qurat ul Ain1, Asifa Khan1*, Asia Riaz2 and Nazia Suleman

...ge survival of adult and larvae of C. septempunctata. Better survival was recorded in beetles stored at 4°C after their removal from cold conditions. Larval survival declined gradually with increased storage length. Most of the larval instars remain alive under storage conditions up to four weeks, but larval survival in third and fourth instar larvae did not extend after three weeks of storage. Our results provide a guid...

Xiaojun Li

...vior of diamondback moth larvae with an approach to the acetone extract effects of Salvia Miltiorrhiza Bunge (SMB). It was found that acetone extracts of Salviami ltiorrhiza Bunge (SIB) represented by cryptotanshinone and dihydrotanshinone had lethal effect on diamondback moth larvae, and the correlation coefficient was 0.972. Through further antifeedant test, it was found that diamondback moth larva...
Asma Ilyas1, Tanzeela Riaz 2*, Sammi Rasheed1, Uzma Ramzan1, Afsheen Munawar Butt1 and Farah Rauf Shakoori1*
Nael Abutaha, Fahd A. AL-Mekhlafi*, Khalid Elfaki Ibrahim, 
Mohammed S. Al-Khalifa and Mohamed A. Wadaan
...rp against Culex pipiens larvae. Hexane extract was prepared from the fruit. The secondary metabolites were identified using phytochemical analysis and gas chromatography-mass spectrometry (GC-MS). The cytotoxic effect was also evaluated using normal human umbilical endothelial cells (HUV-EC). The larvicidal assay was performed on the third instar stage as recommended by WHO. The phytochemical analysis showed the presence of alkaloids, sterols, and phenolic co...

Hossam F. Abou-Shaara

...air the ability of young larvae to develop into pupae. Bee colonies had high sealed brood area and number of bees in yeast group followed by sugar candy and corn flour. Each of corn flour and turmeric affected the survival ability of bee workers negatively unlike yeast and sugar candy. All the feeding types showed approximately the same effect on the ability of bees to tolerate low temperature. Some morphological characteristics (head width, forewing length an...
MAIMOONA KANWAL, MUBASHAR HUSSAIN*, ZAHEER ABBAS1, SHAKIL AHMAD KHAN2, FATIMA
ZAFAR & RIZWANKHURSHEED2
...0, 3.26 and 4.12 g) when larvae were fed on mulberry leaves dipped in 10 % Vitamin C Solution + 2 % Glycine + Alanine (T9).Significant differences were also observed in all growth parameters. Fecundity (439.67 #), Fertility (86.0%) and Hatchability (84.67%) were highest in T9which were significantly different from control (T0), 422.67, 79.33 and 76.33, respectively. Cocoon Weight (1.36 g), Shell Weight (0.33 g) and Cocoon shell percentage (...

ASMA WAHEED QURESHI1* & SHUMAILA ALAM2

...arasitic eggs, cysts and larvae. Seven vegetables including cabbage (Brassica oleracea), lettuce (Lactuca sativa), carrot (Daucus carota), mint (Mentha spicata), chili (Capsicum frutescens), cucumber (Cucumis sativus) and corriander (Coriandrum sativum) were assessed using standard methods. Overall parasitic contamination of 30.7% was observed. Highest contamination was detected in Mardan (36%) followed by Katlang (30%) while the lowest contamination was obser...

Pham Tan Nha*, Le Thu Thuy

...ion of black soldier fly larvae in the diet of Muscovy ducks at 7-12 weeks old. A total of 200 Muscovy ducks were randomly assigned to five dietary treatments. The treatments named as following: CTL - control diet with fishmeal; LP20, LP40, LP60 and LP80 as replacing 20%, 40%, 60% and 80% of fishmeal protein with larval protein (LP) in the control diet with four replications, each replication with 10 ducks (5 males and 5 females). The results showed that the w...
Kalaiselvi Natarajan1, Karal Marx Karuppiah1, Sheena K Baby1, Sakthivel Mohammed2, Shanmugam Seerappalli Aran1 and Suresh Eswaran1*
...ween 0.6 and 0.8 mm. The larvae hatched out after 72 h of fertilization. The total length of the larvae ranged from 2.7 to 2.9 mm. This study aims to analyze the application of RAS technology for year-round seed production of Koi carp. Further this study aims in developing sustainable aquaculture practices to facilitate aquaculturists.

...

Wali Muhammad Mangrio1*, Faheem Ahmed Jatoi1, Hakim Ali Sahito1, Fahmeeda Imdad Sahito2, Bhugro Mal3 and Naseem Qureshi4

... neem oil caused maximum larvae reduction % in all three replications at (50.25%), (43.30%), (34.04%) overall (42.53%) in both years. Neem leaves extract (40.95%), (35.83), (34.08) overall (36.95%). C. gigantea (30.43%), (23.60%), (22.60%) overall (25.54%), and C. colocynthis (25.19%), (23.43%), (26.25%), overall (24.96%). There was significant effect of all the botanical insecticides but neem oil proved with best efficacy against this potential pest, hence re...
Muhammad Shakil Ahmad1, Muhammad Afzal1, Liu Yu Feng2
Muhammad Zeeshan Majeed1*, Hina Safdar3, Arif Mehmood1, Shahid Iqbal4 and Muhammad Adnan1
...tions against 3rd instar larvae of S. litura. Bioassay with botanical formulations showed a significant toxicity of oil and extract formulations of neem (Azadirachta indica) causing 70–77% larval mortality in 72 h observation and exhibiting minimum medial lethal concentration (LC50) and time (LT50) values (i.e. 12.32 and 38.01 ppm and 16.67 and 11.68 days, respectively). Among microbial formulations tested, S. litura-nuclear polyhedrosis virus (NPV) and ...
Syed Said Badshah Bukhari, Ghulam Ali Bajwa and Miskeen Ali
...±0.22 kg per 10,000 larvae) and cocoon weight (1.42 ±0.03 g), while hybrid C02x05M was superior in the shell weight (0.37±0.01 g) and shell ratio (26.47±0.04%). The EI values ranged between 40.96 and 61.92. All hybrids except 05POx06M resulted in greater than 50 EI values. Four hybrids showed positive MPH for the cocoon yield, cocoon weight and shell ratio, while three hybrids showed positive BPH for the cocoon yield, cocoon weight and shel...
Naveed Ahmed, Tanvir Ahmad Qureshi and Muhammad Hamayoon Khan
...nt. Maximum mortality of larvae was recorded as 33.33 % each in 2.0% and 3.0% concentration after 24 hours, 44.44% in 2.0% concentration after 48 hours and 56.11% in 3.0% concentration after 72 hours. Percent mortality was not significantly different among different concentrations of neem oil but was different significantly from control treatment....
Sardar M. Rafique and Ghulam Ali Bajwa
...ealed that the young age larvae of C-102 strain reared under cooperative house conditions and transported by motorbike gave 5.12% (larval body weight gain), 11.34% (cocoon weight) and 12.69% (shell weight) more than conventional methods of rearing. In case of 206-PO, return of larval body weight gain, cocoon and shell weight were 8.15%, 11.76% and 9.56% higher, respectively. Larvae transported in wax paper lost larval body w...
Sardar M. Rafique and Ghulam Ali Bajwa
... with the development of larvae but AD decreased. Overall maximum and minimum larval body weight gain was recorded in 206-MKD and 207-PO, respectively During autumn season the highest and lowest ingestibility, ECI, ECD and AD were 62.09% (206-MKD, 5th instar), 20.86% (208-PO, 5th instar), 31.24% (208-PO, 5th instar), 60 75% (207-PO, 4th instar) and 40 54% (207-PO, 5th instar), 16.95% (208-PO. 4th instar), 19.26% (208-PO, 4th instar), 40.26 (207-PO, 5th instar)...
G.A. Bajwa and H. Gul
...l body weight in treated larvae was 74 19% to 78.92% over control in case of C. procera and the mixture after 12 and 6 days of treatment, respectively Mean larval period was extended upto 22 days in the mixture treatment while it was 11.5 days in control However, mean pupal period was shortened to 6 days by C fistula as compared to control, which was 12.5 days. 100 percent mortality was caused by A. indica after 3 weeks. The overall maximum morta...
Syed Qasim Shah
...e of one and two day old larvae in upper and lower bars of the grafted frames were studied among the sugar and control groups of feeding. The percentage of accepted larvae that were grafted with dilute royal jelly among group showed statistically difference between acceptance of 1 and 2 day old larvae among the upper and lower bar of the grafted frames. The groups accepted more two-...
Wali-ur-Rehman, Meher Nigar Ashiq and Siraj-ud-Din
...acters in the 5th instar larvae of Chinese (C-102) and Japanese (J-101) varieties of the silkworm, Bombyx mori L. were investigated. The mean high humidity of 94% with the prevailing temperature of 23°C increased the larval weight by 11% each, cocoon weight by 20 and 13%, shell weight by 17 and 10%, fecundity by 8 and 11% and decreased larval mortality by 60 and 14% and pupal mortality by 16 and 62% for C-102 and J-101 respectively. The mean high tem...
G. A. Bajwa and M. N. Ashiq
...ses. For each strain 225 larvae of 4th instar were separated from mass rearing lot and divided into three replications. The rearing of silkworm was carried out at 25 ± 2°C and 70 ± 5% rH. The results revealed that bacterial flacherie caused overall 20.52, 18.5, 16.82 and 5.53 percent mortality in C-102, J-101, 206-MKD and 207-PO, respectively. The mortality did not differ significantly among C-102, J-101 and 206-MKD. Viral grasserie resulted ove...
G. A. Bajwa and G. Zimmermann
..., 6.4%, and 8.0% against larvae of Lymantria dispar in the laboratory. 1st instar larvae of the pest were fed on neem sprayed leaves of Prunus domestica. 100 percent mortality was observed in all neem test treatments within 21 days. In the highest dose (8%) maximum mortality was recorded after 72 hours which may be due to contact action of neem oil formulation. On the other hand, the lower doses caused slow mor...
Wali-ur-Rehman
... DDVP 50% EC killed 100% larvae each in 0.05 and 0.03% doses and 83% larvae in 0.01% dose while Laser 25% EC gave 80, 43 and 33% kill of larvae, respectively in 0.05, 0.03 and 0.01% doses. Malathion showed poor results with 36.6 and 26.6% mortality of larvae in 0.05 and 0.03% doses, respectively. In another trial (a fumigant caused 100% mortality ...
Inayat Ahmad Hafiz
...s show that mortality of larvae and pupae was low and the effect of PFI-1 and PFI-2 was significant on economic cocoon characters of the variety. The chemical analysis of these varieties showed that protein contents of PFI-1 and PFI-2 were higher then in Japanese hybrid and Karyansuban varieties and hence gave better cocoon shell ratio. The cocoon quality did not show much difference and the percentage of good quality cocoons was 89.5....
Hanif Gul and M. Ismail Chaudhry
...rbelidae, Lepidopteral) larvae and pupae, were recorded. Aeolesthes sarta (Cerambycidae, Coleoptera) grubs were found predated by a mite predator. Entomophathogenic fungus attacking all stages of I. quadrinotata and Elaterid predator of A. sarta, pit borer Apriona cinerea (Lamiidae, Coleoptera) were recorded. Observations on the biology of Elaterid predator of A. sarta and A. cinerea in the laboratory sh...
M. Ismail Chaudhry and Ghulam Ali Bajwa
...>Plecoptera reflexa larvae, an important defoliator of shisham plantations. In case of A. indica and M. azedarach seed powder was used for making 10% water extract and for D. alba and C. procera leaves, branches and stem were boiled in water for making 15% and 21% decocations, respectively. The experiment was laid out in randomized complete block design. All test materials gave significant control over control with maxim...
Wali-ur-Rahman and M. I. Chaudhry
...led 100% and Orthene 80% larvae in the same dose within 24 hours. In lower doses of 0.01 and 0.02% antimoultants caused 60 to 75% and insecticides 70 to 80% larval mortality in the same periods. The pest was controlled by spraying Malathion 57% E.C. in 0.17% concentration on the resting caterpillars at the natural enemies of the pest....
M. N. Ashiq
...color and pattern of the larvae and shape of cocoon were taken into consideration for purification of the lines. With this method six pure lines, four of Chinese origin and two of Japanese origin, were evolved. All these lines were stable and bred true for the two characters. They were all suitable for rearing in the spring season....
M.Ismail Chaudhry and Ashiq Ahmad
...d most effective against larvae at 0.05%level and against pupae at 0.1%level. Ekalux gave 92%kill in 0.05%dose and 100% kill in 0.1%dose....
Hanif Gul and M. Ismail Chaudhry
...on revealed that cutworm larvae and pupae were found from cages provided with eggs in the month of May. No stage of cutworm was recovered from cages provided with larvae or pupae of cutworms before the hot summer started. It indicates that the larvae and pupae of cutworm did not survive in hot dry weather of June where as the eggs aestivated successfully....
M. Ismail Chaudhry and Hanif Gul
...ainst 2nd and 3rd instar larvae of shisham defoliator, Plecaptera refexa Guen. (Notodontidae). Both the chemicals in laboratory as well as field trials, in doses of o.02 and 0.04% concentrations gave cent percent mortality of the larvae. The residual effect of the insecticides continued up to about one month and provided cover for two successive and overlapping generations the non-target insects, which do not f...
Pervez Khawaja, I. A. Hafiz and M. I. Chaudhry
...7% mortality of the test larvae with dose of 0.8 x 105, 1.6 x105, and 3.2x105 spores per ml against a natural mortality of 34.4% larvae in the check. The efficacy of the product has been proved against this insect hence it can be used on field scale to control the peat. ...
Parvez Khawaja, I.A. Hafiz and M. I. Chaudhry
...ty by killing 72.5-97.5% larvae in various treatments as against 50% natural mortality in check treatment. The method can safely be used against the pest on larges scale....
Wali-ur-Rehman and M. Ismail Chaudhry
... of 1788,794, 571and 208 larvae per tree, respectively caused complete, heavy, medium and light defoliations....
Bashir Hussain Shah, Hanif Gul and M. I. Chaudhry
...ng complete mortality of larvae in 0.1% concentrations. The out-breaks of the defoliator can effectively be controlled without affecting the other biological control agents or the environment....
Mussarrat N. Ali, Anwar Ahmad Khan and Shakeel Haider Zaidi
...ng complete mortality of larvae in 0.1% concentration. The out-breaks of the defoliator can effectively be controlled without affecting the other biological control agents or the environment. ...
M. Ismail Chaudhry and Wali-ur-Rehman
...y infested by fruit moth larvae in all the localities....
Bashir Hussain Shah, Hanif Gul and M. Ismail Chaudhry
.... gave 100% mortality of larvae in .01%, concentration. Thiodan and Hostathion also killed 100% larvae in highest concentration of 0.05% while in 0.01%and 0.25% concentration Hostathion gave 99% and 98.6%mortality and Thiodan killed 93% and 91.5% larvae, respectively. Lorsban and Gusathion gave 93% and 72.5% morality of larvae in highest concentration of...
M. Ismail Chaudhry and Wali-ur-Rehman
...oths of hibernation. The larvae pupate in May - June for a period of 7-30 days. The life cycle is annual. Brachycorphus nursei Cam. (Ichneumonidae) Sturmia inconspicuella and Podomyia sp. (Tachinidae) were recorded parasitizing bagworms in the defoliated area. Gradual increase in parasite population over took pest population in few years and the insect outbreak was controlled by the natural enemies....
M. Ismail Chaudhry and Bashir Hussain Shah
..., were conducted against larvae of poplar defoliator, Ichthyura anastomosis Steph. in the laboratory. In the first trial there was 100% mortality of larvae within three days of release on poplar shoots treated with 1% and 1.5% concentrations of Dipel and within two days in case of 2% concentration of Dipel. In check there was only 36% natural mortality of larvae. In the second trial...
M. Ismail Chaudhry, Bashir Hussain Shah and Hanif Gul
...oviposition, incubation, larvae and pupae were 2.3, 3.95, 3.6, 22.16, and 10.54 days, respectively. In usceptibility test of A. ypsilon and A. segetum to G. V. from Denmark conducted in laboratory, 100% mortality of both species was observed due to virus infection while 62% and 63% larvae of A. segetum and A. ypsilon. Pupated successfully in check treatment....
M. Ismail Chaudhry and Iqbal Ahmad
...ring growing season. The larvae stitch two or more leaves together with silken threads and feed on parenchyma tissues, leaving network of viens and skeleton of leaves intact. It causes more than 90% leaf stitching when severe.
The moths from hibernating pupae emerge during March and copulate 10-20 hours after emergence. The duration of mating varies from 25 minutes to 8 hours. The moths start laying eggs 12 hours after mating. A single female on an a...
Cristian Mendoza1*, Adriana Celi-Soto2, Ernesto Cañarte3 and George Cedeño-García2
...mber of S. valida larvae, the percentage of fresh damaged roots, and leaf emission were assessed. There were fewer S. valida larvae and damaged roots in the treatments with fruitless bunches and fiber at 180 days and with leaves at 90 days after beginning the treatments, while in absolute control, the larval population rose over time. The application of physical barriers reduced the incidence of root borer atta...

Jagdish Jaba1*, Sumit Vashisth2, Sivakumar Golla1 and Suraj Prashad Mishra1

...perature and H. armigera larvae population exhibited highly significant positive correlation with minimum temperature (r= 0.491**) and morning relative humidity (r= 0.528**). Solar radiation also exhibited and showed a significant positive correlation with leaf webber (r= 533**). 

...
Shawky M Aboelhadid1*, Abdel-Azeem S Abdel-Baki2, Khaled M Hassan3
Samar M Ibrahium4, Saleh Al-Quraishy5, Ahmed O Hassan6 and Asmaa A Kamel1
... 4.29% against house fly larvae. The values of LC50 decreased to 1.50% for GN and 0.32% for the combination of G+S. GO and GN form induced (pupal toxicity) 100% inhibition rate (PIR) in the treated housefly pupae at the concentration of 10%. Meanwhile, GO+SO combination showed 100% PIR at the concentration of 2.5%. Furthermore, the LC50 for GO, GN and GO+SO was 0.22, 0.19 and 0.079 % respectively against larvae of C. pipiens...

Daniel Offiong Etim1*, Etim Johnson Umana1, Idorenyin Asukwo Udo2, Ndarake Eden Ini-Ibehe1

...re inoculated with 5,000 larvae of M. incognita and one gram of S. rolfsii (3 Sclerotia g-3) in a heat sterilized soil. The obtained results showed highly susceptibility of okra to M. incognita and S. rolfsii infection without neem cake and Trichoderma species infestation. Amendment of soil with neem cake at 4.00t/ha in combination with T. viride significantly (P≤0.05) reduced root galling, nematode population and nematode reproduction. Soil amendment with ...

Yusra Karim1, Munawar Saleem Ahmad1, Javed Khan2, Imtiaz Khan3*, Said Hussain Shah2, Syeda Anika Shamsher4, Imran Qazi5, Habib-Ur-Rehman Kakar6 and Wajih Ullah7 

...ious concentrations. The larvae of S. litura and H. armigera   were reared in a laboratory under control. Following tests, the LC50 (96-hours) of the CRY1F protein-crystal mixture against lepidoptera pests was determined to be 158.37 µg/ml for S. litura and 170.73 µg/ml for H. armigera. The endotoxin mixes of CRY1F exhibit considerable potency, causing 100% mortality in S. litura with 500 µg/ml and H. armigera with 600 µg/ml.....

Wali Muhammad Mangrio1*, Yasmeen Buriro1, Hakim Ali Sahito1, Faheem Ahmed Jatoi1 and Fahmeeda Imdad Sahito

...eg;C). The newly hatched larvae were white-brown and gradually turned into dirty-brown at later stages. The 1st instar stage measurement was recorded (0.98±0.31), 2nd (172±0.40), 3rd (2.86±0.52), 4th (0.63±0.25), and 5th (3.54±0.70) mm at (30±2°C). Similarly, at (20±2°C), the 1st instar stage measured (0.45±0.21), 2nd (0.67±0.28), 3rd (1.20±0.37), 4th (2.10±0.45), and 5th (2.9...

Aasma Rasheed1, Dilbar Hussain2, Usama Saleem1, Saddam Hussain3, Zeeshan Javed1, Mashal Shahzadi1, Muhammad Sohail Qadir2, Muhammad Saleem2, Abdul Ghaffar2, Mawra Rafique4, Ayesha Ikram5, Saad Rasheed1 and  Muhammad Asrar1*

...etime. C. septempunctata larvae and adults are both active predators. The present study regarding toxicity of new chemistry insecticides against C. septempunctata was conducted under laboratory conditions. The toxic potential of six synthetic insecticides Emamectin benzoate (200ml/acre), Indoxacarb (175ml/acre), Lufenuron (200ml/acre), Spinetoram (80ml/acre), Chlorantraniliprole (50ml/acre), and Flubendiamide (50ml/acre) were tested on C. septempuctata. The le...

Muhammad Saleem1*, Dilbar Hussain1, Tamseela Mumtaz2, Saeed Ahmad3, Muhammad Ihsan Ullah3, Muhammad Luqman3, Muhammad Shafqat4, Fiaz Hussain5, Muhammad Sajjad6, Kaynat Shahzadi2, Muhammad Jawad Saleem1 and Muhammad Hasnain1

...luatedagainst 3rd instar larvae of H. armigera. Three insecticides viz., pyriproxyfen 10.8%EC,chlorantraniliprole 20SC, emamectin benzoate 1.9EC, and one insect growth regulator (IGR), lufenuron, 50EC was tested against H. armigera larvae by using leaf dip bioassay method under laboratory conditions at Entomological Research Institute, Faisalabad. On the basis of LC50 values chlorantraniliprole and emamectin benzoate was pro...

Muhammad Khubaib1*, Muhammad Salman Khan1, Zia Ur Rahman2, Nafees Ahmad1, Muhammad Atif Haider1 and Mushtaq Ahmad Khan3

... i.e. chicken liver, BSF-larvae, commercial feed and beef mince- having different protein levels were administered to compare their suitability as food for the rearing of C. marulius for 45 days. The fry fed with chicken liver and BSF-larvae showed significantly better result in terms of weight and length gain. The survival rate of C. marulius fed with chicken liver, BSF-larvae, commercial...

Hamayun Arshad1, Muhammad Waris2,3 and Qurratulann Afza Gardner1*

... and Anopheles stephensi larvae. For this purpose, we isolated various bacterial strains from our local environment using standard microbiological techniques. Among those isolates, we found novel strain of L. sphaericus Q001, identified by ribotyping and MALDI-biotyping. The 16S ribosomal RNA gene of this strain is 96% similar to that of highly entomotoxic WHO reference strain 2362. The gene sequence was submitted to Genbank (OQ701385). We isolated the S-layer...

Faheem Ali* and Amjad Farooq

...16. In this study, Aedes larvae were collected by using the entomological dipping method and identified in the laboratory. The results showed mean (±SD) temperature, rainfall, and relative humidity during 2014-2016 ranged 12.88-35.43oC, -0.33-87.43mm, and 36.17-72.17%, respectively. Likewise, maximum Aedes larvae were found during the month of November, with 348.67 Aedes larvae from...

Bushra Shaikh1*, Naeem Tariq Narejo2, Faheem Saddar3, Muhammad Hanif Chandio4, Majida Parveen Narejo5, Urooj Imtiaz2, Athar Mustafa Laghari4, Ghulam Abbas5 and Shahnaz Rashid5

....6 %) seconded by insect larvae (20 %) and 3rd preferred was debris (15.4 %). For breeding analysis values of ova diameter were found to be ranged between 0.55-1.0 mm, the gonadosomatic index (GSI) values were also from to be increasing from 0.71-6.5% and from 0.82-10.28% for both the sexes. These values remained high during April to August. It indicated that the fish spawned once in a year in July. Outcomes of egg estimation during the examination depended on...

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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