Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah
Email | mmshah@ciit.net.pk
...nsformation and in vitro regeneration offered new dimensions of research to improve important crop species including wheat. The tissue culture by itself has increased the crop improvement potential to develop new cultivars with desirable traits through somaclonal and gametoclonal selection. While successful tissue culture is critical for genetic transformation, it has been facing greater impediments in wheat and related crop species. Its su...
Qudsia Khalid, Safdar Hussain Shah*, Faiza Zaeem and Saad Hussain Shah
...kinetin (0.25 mg/l). For regeneration MS medium with 1.0mg/l NAA and BAP + 30g/l D-Sorbitol was used. LiCl adapted line showed significantly higher indices of tolerance than unadapted line at 100, 150, 200 and 250 mM NaCl stress. Adaptation resulted in enhanced accumulation of saturated fatty acids (myristic and stearic acids), while unsaturated fatty acids exhibited variable profiles with enhanced oleic acid and significantly reduced linoleic acid. The
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...ficient and reproducible regeneration system from coleoptilar node of elite maize inbred line was developed. Regeneration from coleoptilar node circumvented tedious procedures of raising the immature embryos as a starting material for genetic transformation. Unlike immature embryos, the development of coleoptilar node is seasonal free and green house independent which would accelerate the work relevant to genetic transformat...
Sarfraz Ahmad, Shaista Koukab, Nayyar Razzaq, Muhammad Islam, Aneel Rose*, and Muhammad Aslam**
... recutita has also shown regeneration potential on marginal lands. Commercial scale production of M. recutita is possible in highlands of Balochistan. However, further studies are required to evaluate different genotypes/varieties, essential oil contents at various growth stages, oil extraction techniques, mechanized flower picking, drying/storage techniques and cultivation practices in the existing cropping patterns....
Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*
COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
...hormonal combinations in regeneration media were studied to determine the best combination for in vitro morphogenesis of grapevine accessions viz., 4494(1) Kowar Sufaid, 4472(12) Harker Agoon and 4492(1) Kowar Naray. When levels of benzylaminopurine (BAP) were increased in the basal MS media for culturing the grape plantlets, there was a corresponding increase in the number of shoots and root mass. Additionally, the shoot proliferation response to increasing l...
Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2
Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
...liver rehabilitation and regeneration. The JFE significantly recovered the histopathological alterations in reno-hepatic tissue by free radicals scavenging and metal chelating abilities due to presence of anthocyanin, flavonoids and β-sitosterol. The JFE’sprotective effects against heavy metals environmental toxicants especially Cr+6 is novel and cheapest; that should be sponsored for ethno-medicinal purpose....
Rizwan Ullah Shah* and Iqbal Munir
...llowed by shoot and root regeneration of Brassica carinata. Seeds were sterilized and germinated, of which one week cotyledon and hypocotyl were used for callus formation and shoot regeneration at five different level of 1-naphthaleneacetic acid NAA (0.05, 0.1, 0.5, 1.0 and 1.5 mg/l) and 6-benzyl amino purine BAP (0.1, 0.5, 0.7, 1.0 and 1.5 mg/l), and root regeneration at four levels of in...
Guo-hai Wang1, Zai-xi Yang1, Pan Chen1, Wei-ning Tan2 and Chang-hu Lu1,*
...Seed dispersal and plant regeneration in particular habitats by frugivorous birds are of great concern. In this study, we recorded the bird’s seed foraging and dispersing behavior of local bird species to examine their role in dispersing seeds of Kmeria septentrionalis, an endangered tree species in a karst habitat in southwest China. Twenty-seven bird species were recorded feeding on its seeds, and fourteen bird species were confirmed as seeds di...
Pin Lyu1, Xiangxian Chen2* andQinlong Liu3*
...ression, skeletal muscle regeneration, control of skeletal muscle fibrosis and muscular tissue re-construction. The skeletal muscular structure and C-reactive protein level could reflect the recovery process after skeletal muscles injury with high sensitivity in groups with the four different rehabilitation methods.
...
Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2
...method led to successful regeneration of shoots on Murashige and Skoog (MS) media containing BAP 3 mgL-1 and developed roots in ½ MS media supplemented with IBA 3 mgL-1 in case of OSCAR cultivar. Successful selection of transgenic lines was achieved using selective antibiotic kanamycin 50 mgL-1. NtSAT4 gene integration into the plants genome was confirmed by PCR using the NtSAT4 gene specific primers. The positive transgenic lines were successfully acc...
Zulekha Zameer, Samreen Mohsin, Ammarah Hasnain, Asma Maqbool* and Kauser Abdulla Malik
Influence of Explant Sources on in vitro Callogenesis and Regeneration in Maize (Zea mays L.)
...oughout the year and low regeneration response. The present study is aimed to investigate the effect of various explants sources for callogenesis and regeneration in maize (Pioner 3025). The main explants under consideration were: three parts of germinated seedlings, mature embryo and split seeds. Each of these explants was evaluated for its ability to form callus and to regenerate subsequently. For callogenesis from differe...
Ye Ge1, Chunmiao Zhao1, Weitian Xie2, Ying Liu2,* and Chunhou Xu1,*
...ns were obtained through regeneration, with a sporogenic rate of 69.1% and a colony number of 2.7×105 CFU/mL after an 80 °C high-temperature treatment. After 7 generations of fermentation, the average pH value was 4.47. The average number of colonies in the culture medium was 18.4. The production of protoplasts lays a foundation for research in microecological preparation.
... 
Aya El-Turkey1, A. K. El-Attar1, A. E. Aboulata1, B. Othman2 and K. A. El-dougdoug2
...ts were transferred into regeneration medium and genetically transformed with the HSV-2gD-PB21 binary vector through Agrobacterium co cultivation. Agro-inoculated tomato plants were tested for the HSV-2gD insertion and transcription using both PCR and RT-PCR and the results were successful in getting insert-containing tomatoes against HSV-2gD. Specific expression of the introduced antigen construct at the RNA level was detected by RT-PCR, using specific primer...
Ahmed K. El-Attar; Samah A. Mokbel; Ali H. Hamed
...v>
elimination and regeneration of infected onion bulbs were determined.
...
Kanwal Haneef1,2, Rakhshinda Habib1,3, Nadia Naeem1,3 and Asmat Salim1,*
Mazhar Ullah and Mohammad Sayyar Khan*
...ination resulted in high regeneration capacities. Amongst the carbon sources, sucrose was most effective both for callus induction and regeneration. ANOVA revealed significant differences (P ≤ 0.05) amongst the callusing media (CM). Maximum callus induction (48.55%) was achieved on CM-2 augmented with 2.5 mg L-1 2,4-D and 4% sucrose. All the carbon sources at 4% concentration in CM-2 showed maximum callus induction. Howev...
Nabila Rasheed1*, Bushra Waseem1, Kanwal Haneef2, Shumaila Usman3, Madeeha Sadiq1, Tabinda Urooj1, Sarah Tarique2 and Dabeeran Zeha4
...l therapeutic agents for regeneration and are a valuable source for stem cell banking. Current investigation shows metabolic disorders are not only responsible for genetic problems and certain disease risk factors in adult life but also have adverse effects on perinatal environment. This study was designed for the comparative analysis of viability and healing properties of GDM affected WJMSCs (GDM-WJMSCs) with Normal controls (N- WJMSCs). Mesenchymal stromal c...
Jiao Ma*
...h the potential for self-regeneration and differentiation into cell types that can play an important role in embryogenesis and embryonic development. So far, seven types of cardiac stem cells with different molecular phenotypes and differentiation potentials have been discovered and studied. Increased proliferation and differentiation of these cells in areas of cardiac ischemia is a significant factor in the repair and enhancement of heart injury. This role of...
Muhammad Shafiq1, Tehseen Ashraf2*, Sehrish Mushtaq1, Naveeda Anjum3, Muhammad Asim4, Muhammad Aqeel Feroze3, Malik Abdul Rehman4 and Marja Aziz3
...Protocol for chili plant regeneration with hypocotyl and cotyledon explants was established. The study was conducted to observe the effect of genotypes, culture conditions and growth regulators on plant regeneration of chili pepper genotypes (Seedex Pepper (SP), Loungi, Tatapuri, and Sanam) grown in Pakistan including. For both hypocotyl and cotyledon explants, SP was found to be the most sensitive among the tested genotypes...
Sumreen Begum, Atta-ur-Rahman and Asmat Salim*
...lopment is imperative to regeneration. The purpose of this study is to analyze systematic identification of genes with respect to nephron lineage and pluripotency during postnatal renal development and at adult stage of rat kidney. In the current study, the mRNA levels of nephron genes were analyzed by reverse transcriptase PCR in neonatal and adult rat kidney tissues. These genes were divided into three categories. Group 1: renal multipotent progenitor genes;...
Muhammad Adeel Qureshi1*, Raza Ahmad2, Bilal Ahmed1, Tanzim Ullah Khan1 and Muhammad Yasin3
...llus induction and plant regeneration of potato (Solanum tuberosum L.). The experiment was carried out in complete randomized design (CRD). Leaves and internodes of potato cv. Desiree were used as explant for callus induction and plant regeneration. Two types of callus induction media (CIM) were used, i.e. CIM1 and CIM2 with concentrations of 2, 4-D 2mg/lit and 3 mg/lit respectively. Among the two different callus induction ...
Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5
...ater potential for shoot regeneration and proliferation in the growth regulator-free MS liquid medium (95.25%) as compared to the solid medium (89.00%). Shoot cultures remained viable for up to 15 days without refreshing the medium and could be sustained for 12 months when sub-cultured every 15 days. Invigoration of albino shoots with fresh MS basal medium resulted in multiple green buds, which successfully transformed into 54.60 and 652.78 healthy shoots in 3...
Najamuddin Solangi1*, Mushtaque Ahmed Jatoi1, Adel Ahmed Abul-Soad2, Abdul Aziz Mirani1, Muhammad Aslam Solangi3 and Ghulam Sarwar Markhand1
...bryogenesis and plantlet regeneration in commercially important date palm cvs. Begum Jungi and Ajwa. Spikelet explants of spathes (avg. 17, 28, 32 cm) excised at different intervals were used as initial explants. Results revealed that sterilization of spathes with 50% sodium hypochlorite (NaOCl) solution resulted in significantly highest survival, lowest mortality and contamination in spikelet explants. The spikelet explants obtained from immature spathes (avg...
Zahra Abdulameer Al-Zayadi1, Hana Kadum Shanan1, Al Salihi Karima Akool2*
...t groups, accompanied by regeneration related to the treatment dose. Severe damage comprising necrosis of the hepatic cells, renal tubules, congestion, and infiltration of inflammatory cells was seen in the chemotherapy-treated group and mice suffering from cancer without treatment. This study also revealed variations in serum liver enzyme values between treatment groups. The values of ALT, AST, and ALP (U/L) evaluation in control groups were ˃ 29.01±1...
Moazzam Baig*, Sohaib Ahmed and Arz Muhammad Umrani
...e animals harm the local regeneration as well as the local land cover. The proposed Hypothesis shows that the significant relationship between Dependent and Independent variables i.e. Forest Degradation and Socio-Economic Factors....
Rasema Majeed, Alaa Kamil Mahmood*
...congested blood vessels, regeneration of islet cells. fourth group was administered 81.7 mg/kg BW of ginger ethanolic extract loaded with chitosan nanoparticles orally for 45 days and showed normal section of islet cells, regeneration of islet cells also showed no clear lesions in the islet cells. whereas fifth group was administered 81.7 mg/kg of chitosan nanoparticles orally for 45 days and showed few destructions and vacu...
Ahmed Kadhim Munahi1* , Hameed A. Al-Timmemi2
...particles on spinal cord regeneration in dogs. For this objective, sixteen healthy mongrel dogs were used. They were divided into two equal groups (n=8) at random. All dogs underwent dorsal laminectomy and left lateral hemisection at the level of the second lumbar vertebra. In the control group, the hemisections were treated with 0.2 mL of phosphate buffer saline. Chitosan nanoparticles were applied to the chitosan group with hemisection stie. From the first w...
Qamer J. Jadoaa, Raffal A. Omar*
....75% Diclofenac, on bone regeneration. Forty-five adult male rabbits of the local breed were employed to create a 3.5mm hole defect in the proximal third of the medial aspect of the tibia by an electric drill with dropping isotonic normal saline to prevent thermal necrosis. The experimental animals were divided randomly into three equal groups, each group include fifteen rabbits (n=15). control without any additive. group 1(lidocaine hcl). Which applied daily ...
Shamsa Jabeen and Javed Iqbal Qazi*
...n for increase in muscle regeneration....
Muhammad Sultan1,4, Muhammad H. Mahmood2,4, Takahiko Miyazaki3,4, Shigeru Koyama3,4, Zahid M. Khan1
...siccant/system size, and regeneration temperature etc. Usually it is difficult to measure open-cycle adsorption kinetics precisely in adsorption heat pump systems. Therefore, present study provides an experimental comparison of close and open cycle based water vapor adsorption kinetics for the development of desiccant air-conditioning system. In this regard, a polymer based hydrophilic sorbent was used for close and open cycle adsorption measurements. Gravimet...
Nabaa Fadhil Abbas*, Raffal A. Omar
...toration of turnover and regeneration of epidermal cells to normal levels. The study concluded that tramadol and remifentanil with epinephrine impede burn wound healing in rabbits, highlighting the need for caution in their use.
Keywords | Lidocaine, Remifentanil, Tramadol, Wound healing, Rabbits, Adjuvants
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Mouhamed Zakiou Kolawole Adissa Raimi1, Ghulam Hussain2, Nousheen Zafeer3, Faheem Ahmad1, Muhammad Irfan4, Anwar Ullah1, Imdad Kaleem1 and Asghar Shabbir1*
