Effect of Growth Regulators on Callus Induction and Plant Regeneration in Potato (Solanum tuberosum L.) Explants
Effect of Growth Regulators on Callus Induction and Plant Regeneration in Potato (Solanum tuberosum L.) Explants
Muhammad Adeel Qureshi1*, Raza Ahmad2, Bilal Ahmed1, Tanzim Ullah Khan1 and Muhammad Yasin3
ABSTRACT
The main objective of this study was to find a best protocol for callus induction and plant regeneration of potato (Solanum tuberosum L.). The experiment was carried out in complete randomized design (CRD). Leaves and internodes of potato cv. Desiree were used as explant for callus induction and plant regeneration. Two types of callus induction media (CIM) were used, i.e. CIM1 and CIM2 with concentrations of 2, 4-D 2mg/lit and 3 mg/lit respectively. Among the two different callus induction media (CIM), CIM1 with 2mg/lit auxin (2, 4-D) showed the best results. All the internodal explants placed on CIM1 produced 100% callus while CIM2 produced 70% callus. No callus was observed from leaves on CIM1 and CIM2. After callus induction, four different types of media were used for plant regeneration, having different concentrations of cytokinin (BAP and Zeatin) i.e. RZ1.5, RZ2, RB1 and RB2. The most efficient regeneration media was RZ2, having Zeatin 2mg/lit showed early and maximum shoot formation. On average, 05 shoots per explant were observed on RZ2. No shoot formation was observed with regeneration media having BAP, i.e. RB1 and RB2. From the research work, it is concluded that auxin (2, 4-D) @ 2mg/lit and cytokinin (zeatin) @ 2mg/lit proved to be the optimum quantity for callus induction and plant regeneration in potato respectively.
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