Manzoor Hussain*, Miloslav Zouhar and Pavel Rysanek
...dogyne hapla both in-vitro and in-vivo. All fungi proved to be efficient in reducing final population of northern root knot nematode M. hapla and giving vigour to the plants. In laboratory experiment, L. muscarium was the most effective against nematode eggs (95.6%) and second stage juveniles (J2) (95.8%) infection. In greenhouse experiment, similar trend was found. L. muscarium proved to be more effective against M. h...
Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
...hich are developed by an in-vitro selection process known as “Systematic Evolution of Ligands by Exponential Enrichment (SELEX)” technique. Among the receptors available for biosensing, aptamer exhibit the advantages of high specificity, selectivity, stability, facile labelling and modification, which makes them ideal candidates for development of new biosensing applications for detection of specific target molecules. In the present review, we conc...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...s (CF) were prepared. In in-vitro test single eggmasses of uniform size were kept in 5ml CF in 5cm diameter Petri plates. Antifungal activity was tested by dual culture of wilt fungus with antagonist fungus and bacteria isolates in PDA media plates. In in-vivo test soil was amended with antagonist and wilt fungal and bacterial isolates 2 x 106 CFU/ml and 2 x 108 CFU/ml respectively as talc formulation @ 0.2% (w/w). Twenty one days old tomato seedlings were tra...
P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi
Botanicals for control of anthracnose of bell peppers
...ial growth of C. capsici in-vitro. The plant extract from C. aromaticus at 50% concentration showed 42% radial mycelial growth inhibition in poisoned food technique, whereas 15% increased seed germination and 26% reduced disease incidence is recoded when compared to untreated control. In pot experiments, there is an increase in height and weight of the plants by 22% and 44% respectively in seeds treated with C. aromaticus when compared to chemical fungicide Ba...
Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
.../i>in camel milk, and in-vitro therapeutic response to drugs. Two different ecological zones, Cholistan (n=185) and Suleiman Range (n=177) of Pakistan having major population of camel were screened for subclinical mastitis using California mastitis test using convenient sampling technique. Biochemical examination was performed to isolate S. aureus that further were tested Penicillin, Glycopeptide, and Cephalosporin classes of antibiotics for conf...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5
...th a view to optimize an in-vitro culture protocol for propagation of an endangered medicinal plant Neurada procumbens L. from Cholistan desert in Pakistan. Nodal segments from healthy grown plants were used as explants and cultured on standard Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Bu...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
Safina Naz1, Khushbo Hussain1, Muhammad Asif Zulfiqar2* and Syed Atif Hasan Naqvi3
.... Research comprising of in-vitro and field trials was conducted to define the efficacy of growth promoting substances which showed that foliar application of Naphthalene Acetic Acid, Salicylic acid (SA), combination of Naphthalene Acetic Acid & salicylic acid and orange oil extract on physical and chemical parameters gave significant response. NAA promotes vine length upto 12.66 feet while chlorophyll content was 72.50 SPAD Value, while fresh weight of le...
In-vitro Anticancer Activity of Copper (II) and Zinc (II) Complexes with Derived Schiff Base Ligands
Qaisar Jamal1,Salman Ahmad1, Nazma Habib Khan1, Sobia Wahid1, Muhammad Ikram2, Sadia Rehman2, Syed Basit Rasheed1 and Akram Shah1,*
... We aimed to investigate in-vitro anticancer activity of two transition metal compounds of derived Schiff bases; NM-3 (Tetrakis (2-{(E)-[(2- {[(Z)-(2-hydroxynapthyl) methylidene]amino} phenyl)imino] methyl}phenol) Copper(II) and NM-4 (Tetrakis (2-{(E)-[(2- {[(Z)-(2-hydroxynapthyl) methylidene] amino}phenyl)imino]methyl} phenol) zinc(II)), against human THP-1 leukemia cell line in-relation to miltefosine (standard chemotherapy). Mean % inhibition for NM-3 again...
Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*
...was carried out, include in-vitro antibacterial and antifungal screening of aqueous methanolic extracts as well as crude saponins isolated from leaves and roots of Sarcococca saligna, using disc diffusion method. The tested bacterial strains include, B. subtilis, E. coli, S. aureus, P. fluorescens and fungal strains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.00±0.56 mm and 25.00±0.50 mm was given by et...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
Mohammed Al Bratty1,*, Hassan A. Alhazmi1,2, Desam Nagarjuna Reddy3, Abdul Jabbar Al-Rajab3, Sadique A. Javed1 and Zia ur Rehman1,4
Muhammad Naeem Khan* and Asad Jan
...us solvent fractions and in-vitro antimicrobial activities of whole plant of D. botrys. Qualitative phytochemical screening of MCE and solvent fractions of D. botrys showed the presence of alkaloids, phenols, flavonoids, saponins, tannins and sterols, however in n-hexane fraction (HxF) only flavonoids and saponins were detected. In quantitative analysis, among all the solvents, ethyl acetate fraction (EAF) had highest amount of phenol (27.4 mg/g), flavonoids (...
Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui2
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
Qaiser Shakeel1*, Rabia Tahir Bajwa1, Yasir Iftikhar2, Mustansar Mubeen2, Muhammad Luqman3, Waqas Ashraf1 and Ifrah Rashid4
Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3, Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
... of risk factors, and in-vitro antibiogram of non-biofilm producing S. aureus (nbpSA) and biofilm positive S. aureus (bpSA)from mastitic goats. The purposive sampling technique was applied to collect n=200 milk samples from different regions of goat populated areas of district Faisalabad-Pakistan. Using surf field mastitis test, collected milk samples were screened for subclinical mastitis at the spot for subsequent identification of patho...
Muhammad Shakil Ahmad1*, Muhammad Afzal1, Sylvain Nafiba Ouedraogo2 and Muhammad Zeeshan Majeed1
...nd Okara districts. This in-vitro study assessed the comparative feeding preference, food consumption and utilization rates and larval development of S. litura on different white and red skinned potato cultivars. Feeding bioassays revealed that all parameters including leaf weight loss, larval weight gain, relative food consumption and growth rates and conversion efficiency of the ingested food were statistically significant (P ≤ 0.05). Exotic potato cultiv...
Misbah Ali1, Safdar Ali1, Muhammad Ahmad Zeshan2*, Rana Binyamin3, Nadeem Ahmed4, Muhammad Usman Ghani5 and Awais Ahmed Khan2
...nst C. graminicola under in-vitro and in-vivo conditions. The poisoned food technique was used for in vitro trials with three concentrations (150 ppm, 200 ppm, and 250 ppm). The most effective concentration of fungicides from laboratory experiment was used in field conditions against red leaf spot of sorghum. Results showed that Cabrio Top was the most effective fungicide by showing 88% fungal inhibition while Native also exhibited good results by showing 78.1...
Lala Rukh1, Muhammad Nadeem1, Tusneem Kausar1, Mian Anjum Murtaza1, Muhammad Luqman2*, Muhammad Bilal Shahid1 and Amal Shaukat3
...ed for physico-chemical, in-vitro starch and protein digestibility, antioxidants and microbial count. Results showed that date bars are rich source of protein, fiber, carbohydrates and fat with good digestibility properties. Date pit powder and soy protein isolate incorporation enhanced the total antioxidants and DPPH activity in soy date bars however, decreasing trend was observed in total antioxidants and inhibition of DPPH radical activity during 90 days of...
Tahsin S. Shoala1, Ahmed K. El-Attar2, Ahmed I. Abd El Maksoud3
Muhammad Madni Afzal1*, Shahbaz Talib Sahi1, Amer Habib1, Waqas Ashraf2, Muhammad Ahmad Zeshan3, Muhammad Raheel2 and Qaiser Shakeel2
...esign (CRD) was used for in-vitro study and randomized complete block design (RCBD) for greenhouse and field experiments. Three desert plants i.e. Gossypium thurberi, Calotropis procera and Suaeda fruticose were used at 50 ppm, 100 ppm and 150 ppm concentrations. Maximum basal rot disease reduction (78.6%) was recorded at 150 ppm concentration of wild cotton (G. thurberi) under in-vitro conditions. Whereas, wild cotton also ...
Anwar Khan1, Fareeha Nosheen2,4, Bushra Tabassum2*, Olawale Samuel Adeyinka2, Khurram Shehzad3, Naila Shahid2, Arif Muhammad Khan4 and Idrees Ahmad Nasir2
... inhibition of PVX in in-vitro assay. Down regulation of potato virus X through coat protein gene targeted siRNA is a potential way of inhibiting invading virus and to effectively stop its systemic spread.
...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
...f C. tuberculata through in-vitro organogenesis was carried out at in-vitro lab, Bio-resources Conservation Institute, NARC, Islamabad, Pakistan. Nodal-tips as explants were grown on MS salts enriched with diverse plant growth regulators including BAP, NAA, Kin and 2,4-D, alone as well as in combinations. The highest growth response for callusing 78.3% was observed in BAP (0.5mg/L) in combination with Kinetin (0.25mg/L) +NAA...
Jarmuji Jarmuji1,3, Lili Warly2*, Mardiati Zain2, Khasrad Khasrad2
... sakura block plus as an in-vitro supplementary feed in Oil Palm Fronds (OPF) feed on optimizing rumen fermentation products, digestibility of nutrients, and gases production. Sakura block plus is a modification of commercial sakura block by using earthworm flour as a source of protein as a substitute for rice bran. The treatments were arranged as follow: P0= OPF + concentrate + 10% commercial sakura block, P1 = OPF + concentrate + 6% sakura block plus, P2 = O...
Indah Prihartini1*, Miftachi Ari2, Manar Fayiz Mousa Atoum3,4, Akhis Soleh Ismail1 and Listiari Hendraningsih1
...P3: 1.5 %). Also, the in-vitro gas production was observed at 2 h, 4 h, 8 h, 16 h, 24 h, 36 h, 48 h, 72 h, and 96 h incubation period for digestibility and efficiency of microbial protein synthesis using the in-vitro method. The result showed that treatment significantly affected (P < 0.05) gas production, digestibility, and efficiency of microbial protein synthesis (ESPM). The highest gas production,...
Soliman Mohammed Soliman
...ca schidigera) on in-vitro total gas and methane production, in-vivo rumen fermentation parameters, and milk production in dairy cows. Twenty Friesian cows were randomly allocated to four groups (five cows /group). All cows were fed TMR (total mixed rations). The first group was fed TMR without supplementation, groups from 2 to 4 were fed TMR supplemented with one of the feed additives S. cerevisiae, yucca, or green seaweed at a r...
Hassan Ali1, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1* and Muhammad Imran Hamid2
...obial insecticides. This in-vitro study assessed the anti-insect potential of four local plant extracts and two promising microbial formulations against 5th instar larvae of T. granarium. Toxicity bioassays revealed that the extracts of Citrus reticulata L. and Solanum nigrum L. were most effective against T. granarium causing significantly higher larval mortality (35 – 40%) than other botanical treatments. Similarly, the highest concentration (15%) of C...
Saima Mehar1 and Salma Javed2*
...f plant extracts. During in-vitro condition inhibition of egg hatchability and J2 juveniles’ mortality varied according to the concentration of plant extract. WC=1 showed 100% mortality after 48 hrs of exposure at 1% with the lethal concentration (LC50) values 0.168 mg/land all four concentration are 100 % toxic at 72 h of exposure. W-56D proved to be 100 % lethal at 0.1 and 0.5 % concentration after 48 h of exposure with (LC50) 0.065 mg/l. The lowest mo...
Imtiaz Ahmed*, Imran Khan and Zia Ud Din
...ctively along with“in-vitro starch digestibility”. The ash, fiber and protein content increased significantly while the carbohydrate content had significantly lower values (p, for all trends < 0.05) as the ratio of incorporation increased when compared with the control sample (data not shown here). The CWB also showed significantly (P<0.05) higher phenolic and total flavonoids content. Furthermore,CWB showed significantly (P<0.05) higher a...
Bibi Hajira*, Imran Khan and Zia-ud-Din
...flours in white bread on in-vitro starch digestibility, estimated glycemic index (eGI) and consumer acceptability. Bread was formulated by incorporating blends (equal proportion) of sorghum and barely flours at 20%, 30%, 40% and 50% levels, each. Composite bread was compared with control bread (CB) made from 100% wheat flour only. In-vitro starch hydrolysis was determined enzymatically at 0, 20, 30, 45, 90 and 120 min. Rapid...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
...ays associated with G9a, in-vitro and in-vivo safety analyses is required to fully establish G9a as a therapeutic target for cancer progression and UNC0642 as an anti-cancerous therapeutic agent for the treatment of breast cancer. ...
Tarek A. Wrshana1, Yousry A. Dowidar1, Bahgat A. El-Fiky2, Ali M. El-Rify1, Walaa A. El-Sayed3, Basem M. Ahmed4*
...it SARS-CoV2 replication in-vitro. In this study, MSCs were prepared from the bone marrow of native Egyptian Fayoumi chicken. The MSC with the surface marker CD105 (CD105+) were magnetically separated and infected with virulent Newcastle disease virus (vNDV). The virus-induced multiple changes at the cellular and ultrastructural level in the infected cells, and it was able to maximize the production of interferon-gamma (IFNγ) and interleukin 2 (IL2), int...
Hassan Raza1, Muhammad Zeeshan Majeed1*, Muhammad Irfan Majeed2, Mujeeb-Ur-Rehman1 and Muhammad Asam Riaz1
...ment. Results of initial in-vitro trials indicated that formulated beads remained intact and did not disintegrate by consecutive episodes of wetting and drying and were able to attract more than 50% termite individuals exposed. This formulation suppressed the termites in field as no termite foraging was recorded inside the bait-treated monitoring stations for about a month post-treatment, while the termites fed on all beads in the control monitoring stations. ...
Sajjad Khan, Fahad Karim Awan*, Zulfiqar Ali Gurmani, Muhammad Usama Hameed, Allah Bakhsh and Kainat Bibi
...ltivars. For the purpose in-vitro study was conducted at NARC fodder laboratory to study the response of two oat cultivars namely NARC and PARC Oat against drought and salinity stress. Salt concentration of 80 mmolLˉ¹ showed non-significant effect on germination % and shoot length of both oat cultivars except salt concentration of 120 mmolLˉ¹, but tolerance in NARC, Oat is higher than PARC oat. Salt concentrations positively increased root length, ...
Hina Safdar1,2*, Nazir Javed2, Sajid Aleem Khan2, Muhammad Zeeshan Majeed3, Arif Mehmood3 and Muhammad Arshad4
...atment. Findings of this in-vitro study demonstrate that EPNs and their symbiotic bacterial isolates would be effective biorational control tools against S. litura....
Muhammad Shakil Ahmad1, Muhammad Afzal1, Liu Yu Feng2, Muhammad Shahroaz Khan1 and Muhammad Zeeshan Majeed1*
... Overall results of this in-vitro and in-situ evaluation demonstrate the effectiveness of biorational insecticidal formulations and recommend their incorporation in integrated management of S. litura.
Novelty Statement | Laboratory and then field evaluations of some promising botanical, microbial and non-conventional synthetic insecticidal formulations against 3rd instar larvae of armyworm (Spod...
Jie Chen, Qinghua Qiu, Yanjiao Li, Xianghui Zhao, Lanjiao Xu, Xiaowen Xiong, Qingqi Wen, Mingren Qu and Kehui Ouyang*
...ocytes were isolated and in-vitro adipogenic differentiation was performed in high-glucose medium (4500 mg/L glucose, HGM) or low-glucose medium (1000 mg/L glucose, LGM). Lipid contents in adipocytes were determined by oil red-O staining. Autophagy, adipogenic differentiation and fat metabolism were evaluated by western blot. Subcutaneous pre-adipocytes proliferation rate, differentiation degree and lipid accumulation were higher than that of visceral pre-adip...
Ghada A. Ibrahim1*, Mohamed Sayed Helal1, Nahed Abd Elhafeeze Kamoura2, Mohamed Samir3,4, Amira Mohamed Mazid5, Mohamed F.M. Farag6
AISHA, MUHAMMAD ASAM RAZA* AND UMME FARWA
USMAN AHMAD*1, AMTUL JAMIL SAMI1, MADEEHA KARAMAT1, MADEEHA KHALID1, FAIZAN NAEEM2 & MUHAMMAD JAMIL YOUSAF3
...ion discharge instrument in-vitro and in-vivo. Fickian diffusion mechanisms have been read for the release of insulin at diverse pH (5.4 and 7.5).
SYED ALI RAZA1, MUHAMMAD WASEEM MUMTAZ2*, AYOUB RASHID CH1, ABDUL WAHEED1, JAMES WILLIAM3 & MUHAMMAD ARSHAD1
....41 ± 2.45%). The in-vitro anti-obesity attribute of extracts was assessed by measuring the inhibition of enzymatic activity of pancreatic lipase. The 60% ethanolic leaf extract exhibited maximum pancreatic lipase inhibition of 54.55 ± 0.72% but lower than standard drug orlistat (66.65 ± 0.68%). Findings explored the hidden antioxidant and pancreatic lipase inhibitory potential of Conocarpus lancifolius that may be exploited for nat...
Amirul Faiz Mohd Azmi1,4, Hafandi Ahmad1, Norhariani Mohd Nor1, Goh Yong Meng1, Mohd Zamri Saad2, Md Zuki Abu Bakar1, Norafizah Abdul Rahman5,6, Agung Irawan7,9, Anuraga Jayanegara8,9, Hasliza Abu Hassim1,3,9*
...ations, especially in an in-vitro study. Even though several studies have reported the potential of supplementation in enhancing the growth of buffaloes, the effect on a different breeds of buffaloes, the potential to reduce methane production, and the changes in microbial populations remained unclear following different ratios of forage: supplementation feeding regime. This study described the effects of supplementing Brachiaria decumbens grass (G) received e...
Elihasridas, Mardiati Zain*, Roni Pazla, Simel Sowmen, Qurrata Aini
...ed by incubating them on in-vitro media at 39ºC/48 h. The analysis of variance following a 5 x 4 randomized block design was used. The results revealed a significant (P<0.05) increase in the digestibility of ammoniated aromatic lemongrass waste in the rumen. The degradation of ammoniated aromatic lemongrass waste was found to be significantly (P<0.05) higher when supplemented with cassava leaves and mineral zinc (T3) compared to others. However, it...
Tabark F Ahmed, Nibras Z. Yahya*
...oxacin and erythromycin) in-vitro and the in vitro. The research was included two experiments. The first one was to isolate and identify E. coli O157; E. coli sample was taken from a hospital, the sample was cultured on MacConkey agar, Sorbitol MacConkey agar, chrome agar, eosin methylene blue agar, then VITEK® 2 System was performed Data showed the sample was positive as a result of E. coli O157. The second experiment examined the pharmacodynamics of asco...
SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN
...erapeutic values through in-vitro biological assays.
Quantitative analysis was done on powder of Misopates orontium by
using standard methods for estimating the primary and secondary
metabolites. The maximum value of total carbohydrates (11.04μg/ml),
total starch (14.69μg/ml), total protein (14.34μg/ml), total amino
acids(28.43μg/ml), total lipids (3.67 μg/ml), total glycosaponins (14.9...
Firda Dimawarnita1*, Edwina Gabriela Tesalonika Mustamu2, Yora Faramitha1, Haryo Tejo Prakoso1, Wildan Aulia Noorsy1, Komang Gede Wiryawan2
...ignification process and in-vitro rumenal fermentation. The treatments were raw EFB (P0) and delignified EFB (P1). We evaluated rumen pH, total volatile fatty acids (VFA), ammonia concentration (NH3), dry matter digestibility (DMD), crude fiber digestibility (CFD), organic matter digestibility (OMD), methane gas production, fulvic acid content, and bacterial population. The results indicated that the treatment did not have a significant effect on DMD, OMD, pH,...
Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*
..., the aim of the current in-vitro study was to determine the interaction between chicken erythrocytes and M. synoviae using Transmission electron microscope (TEM) and further to investigate the mRNA gene expression of MyD88 (Myeloid differentiation primary response 88), CCL5 (C-C Motif Chemokine Ligand 5), MDA5 (melanoma differentiation-associated protein 5) IKBKE (inhibitor of nuclear factor kappa-B kinase subunit epsilon), NFKBIA (NF-kappa-B inhibitor alpha)...
Xin-yu Han1,2, Afrasyab Khan1,2, Ali Raza Jahejo1,2, Qian-qing Cheng1,2, Meng-li Qiao1,2, Raza Ali Mangi1,2, Muhammad Farhan Qadir1,2, Ding Zhang1,2, Ying Wang1,2, Yu-hai Bi1,3, Rui-wen Fan1,2 and Wen-xia Tian1,2*
...e purpose of the current in-vitro study was to determine the chicken erythrocytes interaction with M. synoviae using a transmission electron microscope (TEM). The mRNA gene expression of TLR1, 2, 3, 4, 5, 7, 15, MHC I, II, and MyD88 in M. synoviae infected chicken erythrocytes was determined using quantitative real-time PCR (qRT-PCR) at four different time intervals (0, 2, 6 and 10 h) post-infection and compared to uninfected controls. The mRNA expression of T...
MAHRUKH SHAHID1, KHAJISTA JABEEN1* & SUMERA IQBAL1
...rations were experienced in-vitro against tested fungal strain. All the applied concentrations of methanol extract i.e. 1%, 2%, 3%, 4%, 5%, 6%, 7% and 8% effectively inhibited F. oxysporum growth. However, 7% and 8% concentrations were proved to be most efficient in retarding the test fungus growth upto 15%. Partitioning of biochar methanolic extract was done using some organic solvents such as n-hexane, chloroform, ethyl acetate and n-butanol in increasing th...
Putri Okta Shafura1, Mardiati Zain2*, Elihasridas2, Bima Bagaskara1, Ummi Amanah1, Laras Sukma Sucitra1, Bella Veliana Utami1, Roni Pazla2, Erpomen2, Ezi Masdia Putri3, Rusmana Wijaya Setia Ningrat2, Riris Delima Purba3, Ruslan Abdul Gopar3, Putut Suryo Negoro3
...r). The Tilley and Terry in-vitro methods were utilized to measure rumen characteristics, nutrient digestibility, protozoan population, microbial protein synthesis, and methane gas production. The results revealed a significant impact (P < 0.05) of these treatments on nutrient digestibility and rumen fermentation characteristics. The combination of 0.5% S. cerevisiae and 0.3% sulfur tended to increase nutrient digestibility, total VFA production, and microb...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...h 2 mM selenium improved in-vitro (chilled and frozen-thaw sperm traits) and in-vivo conception rate of Kamohri buck semen. ...
Waseem Abbas, Imtiaz Ahmed* and Imran Khan
Farrukh Avais1, Anser Ali1, Hamza Javed2, Muhammad Saleem3, Tehreem Tahir4,
Muhammad Rafiq5,*
...t
activities in-vitro. Our results confirmed that all MeOH-crude AS and MP extracts
showed significant anti-oxidant activity with IC50 values ranging from 0.05 ± 0.2 mg/ml
to 4.3 ± 2.3mg/ml. Moreover, AS and MP all 16 extracts have significant anti-tyrosinase
activity with IC50 range from 0.014 ± 0mg/ml to 1.205 ± 0.07mg/ml. Interestingly, AS
leaf MetOH_EA, AS leaf MetOH_CH...
Ashar Farooq1*, Mohammad Salim2, Muhammad Tahir Khan3, Zahid Rauf1, Ahmed Hussain2 and Muhammad Bilal Zia1
Tanvir Hussain
...en in 2001 to assess the in-vitro culture potential of water hyacinth by taking different plant-parts (root, stem, leaf, anther, stolon, ovules, embryos/embryos with stem) as explants in different culture media i.e. Murashige & Skooge (MS) medium, Gamborgs B5-salt (B5)and Basal nutrient medium (BNM) alone and in combination with Auxins and Cytokinins. It was found that only the axillary buds gave good response to all media. For callus initiation, same ex-plant...
Hina Ilyas1, Umar Hanif2, Anser Ali1, Zahid Hassan Tarar3,Hamza Javed4, Tehreem Tahir5, Muhammad Rafiq6*
...ti-tyrosinase potential, in-vitro. Our results confirmed that all C. bergamia and F. carica crude extracts showed significant anti-oxidant activity with IC50 range of 384.2 ± 19.1 to 77.3 ± 10.0 μgmL-1, collectively. Moreover, significant anti-tyrosinase activity for C. bergamia (IC50 range = 4.1 ± 0.3 to 366.8 ± 36.5 μg mL-1) and F. carica (IC50 range = 156.5 ± 12.4 to 15.1 ± 2.9 μg mL-1) was found . Interest...
Hafiz Muhammad Usama Shaheen1, Nasir Ahmed Rajput1*, Muhammad Atiq1, Ghalib Ayaz Kachelo1, Hadeed Ahmad1, Muhammad Wahab1, Muhammad Faran Tahir1 and Abuzar Hasnain2
...eventing B. oryzae under in-vitro, from where only 8 most effective phyto-extracts were further investigated at different concentrations (5, 10, and 15%) in-vitro using poisoned food technique. From in-vitro experiment, two highly effective extracts were demonstrated for their substantial response against B. oryzae under greenhouse conditions, separately and in combination by using three a...
Muhammad Qasim1, Muhammad Zeeshan Majeed1*, Muhammad Arshad1, Umair Abbas1, Mehar Zubair Shehzad1 and Abu Bakar Muhammad Raza1,2
Muhammad Muneeb1, Ali Hussain2*, Qurat-ul-Ain Ahmad3, Arshad Javid1 and Jibran Hussain3
...a co-culture to treat an in-vitro prepared sulphate-rich wastewater while employing various agro-industrial organic waste as economical growth substrates. A combination of sulphate-reducing and cellulolytic bacteria in a ratio of 1:1 (v/v) was proved to be efficient for the reduction of sulphate in controlled as well as in the experimental conditions. The implicated microbial co-culture reduced 96 and 93 % of the added sulphate (5 gL−1) while using rice ...
Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*
... supplementing LC on the in-vitro fertilization (IVF) of Egyptian buffaloes. We evaluated blastocyst and apoptosis rates in order to substantiate the potential development of cumulus oocytes–complexes (COC) and determine the effect of LC in reducing the occurrence of apoptosis under in vitro maturation (IVM) conditions for buffalo blastocysts. The tissue culture media included a control culture medium and other three LC supplemented media (2, 3 or, 4 mM ...
Muhammad Shahkar Uzair1, Muhammad Mushtaq1, Muhammad Shuaib1*, Saqib Nawaz2, Mehboob Ali2, Faiz ur Rehman2, Abubakar Sufyan3, Usman Zeb4, Muhammad Ayaz5 and Muhammad Aamir Khan5
...the finisher stage. Both in-vitro and in-vivo studies were carried out to evaluate the antimicrobial activities and potency of this combination. A total of one hundred and eighty day-old chicks were randomly allotted to 6 groups i.e. G1 (negative control), G2 (positive control), G3 (standard antibiotic neomycin only), SN-1 (Serratiopeptidase @40g/L+Neomycin@10mg/L), SN-2 (Serratiopeptidase @50mg/L+ Neomycin @20mg/L), and SN-3 (Serratiopeptidase @ 60mg/L+ Neomy...
Asfand Yar Khan1, Syed Saleem Ahmad1*, Muhammad Avais1 and Kamran Ashraf2
...li O157:H7) based on the in-vitro and in-vivo trial. A total of twenty-one (n=21) PCR-confirmed isolates were selected for detection of virulent genes in-vitro trial (zone of inhibition). For in-vivo trial, forty-two (n=42) day-old chicks were divided into seven equal groups randomly (n=6 birds/group), and were classified as: G1 (Azadirachta indica @ 18.25 mg/ml), G2 (Melia azedarach @ 15.75 mg/ml), G3 (Withania coagulans @ ...
