Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan
...ntitative real-time PCR, cytokines gene expression were examined in response to infection with two strains of Egyptian H9N2 (namely V3 and RSF/1) in chicken peripheral blood mononuclear cells (PBMC). Hemagglutinin gene sequence analysis of the two strains reveal high similarity with difference close to cleavage site (HARSSR/ GLF) and (PARSSR/GLF) for V3 and RSF/1 respectively. Results of growth kinetics in PBMCs showed that V3 replicated more efficiently compa...
Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns
...nduces the production of cytokines, chemokines and interferons. Through Janus kinase/signal transducers and transcription activators pathway, interferon induces the transcription of interferon-stimulated genes (ISGs), responsible for playing an essential and decisive role in the virus pathology. Some studies have already reported the overexpression of these genes against IBDV infection in susceptible cells. The protection ability of ISGs to safeguard chicken c...
Rehana Shahida1, Tasnim Farasat1, Shagufta Naz1,* and Shahjahan2
...tionship of inflammatory cytokines with insulin resistance and the molecular mechanisms involved in the interaction of atherosclerosis and hyperglycemia within the context of chosen pro atherogenic parameters including plasma glucose level, serum levels of insulin, IL-6, and TNF-α. Serum level of thrombomodulin was assessed as a measure of vascular damage. Different demographic parameters as age, BMI, waist /hip ratio, B.P., personal history and socioeco...
Yanjie Guo1, Weini Wu1, Hongmei Wang2, Xiao Guo2 and Yongping Xu2,*
...plore whether or not Th1 cytokines influence the physiological state of the female reproductive system by sympathetic nerve pathways, we used immunohistochemistry, RT-PCR, Western blot and in situ hybridization to detect the expression and distribution of IL-18Rα in the inferior mesenteric ganglion of the female goat. Our results showed that IL-18Rα mRNA and proteins are expressed in the IMG, which provides molecular evidence for the study of immun...
Jiji Li*, Tianyang Zu, Xiangli Dong, Xiao Yang, Wei Liu and Changwen Wu
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Xiaojie Ding, Xiling Sun, Zuien Wang, Qiusheng Zheng, Xiaofei Yu, Wenjin Hao, Kejun Wang, Wenjuan Xu and Zhengping Dong*
...ease of pro-inflammatory cytokines and the bacterial endotoxins by blocking the transmission of TLR4/9-NF-kB signaling pathway so that Wumei Pill has a significant therapeutic effect on IBS-D.
...
Tünay Kontaş Aşkar1,*, Şinasi Aşkar1, Olga Büyükleblebici2 and Murat Güzel3
... adenosine deaminase and cytokines (TNF-alpha, IL-1β) were determined and compared with the healthy controls. Significantly high levels of plasma malondialdehyde, nitric oxide, myeloperoxidase, adenosine deaminase and cytokines and significantly low levels in total antioxidant capacity and glutathione levels were found in dogs with leishmaniasis when compared with the healthy controls. According to the result of this st...
Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*
...l-like receptors (TLRs), cytokines, and avian β-defensins (AvBDs). Host defense peptides (HDPs) are important effector molecules in innate immunity. In this study, thiram has been used to induce tibial dyschondroplasia (TD) and the chickens were injected with different doses of recombinant glutathione-S-transferase A3 (rGSTA3) proteins. We explored the responses of HDPs in erythrocytes to thiram-induced TD and rGSTA3 protein by quantitative real time PCR ...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...rogenase (LDH) activity, cytokines (TNF-α, IL-1β, IL-6) concentrations, urinary volume, creatinine, and both serum and urinary electrolyte levels were measured. In addition, the apoptosis rate of white blood cells was analysed from plasma samples. Tissue samples from the brain, heart, aorta and kidneys were used for analysis of the collagen content besides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant d...
Rida Younas1*, Tamseela Mumtaz1, Nabeela Roohi2 and Muhammad Amir Iqbal3
...tween serum inflammatory cytokines (IL-6 and IL-8) level and post predicted FEV1%, FVC% and FEV1/FVC was analyzed. Results of serum IL-6 and IL-8 showed significant difference among control and GOLD stages II, III and IV (both P< 0.05). The level of IL-6 was highest in GOLD stage II and lowest in stage III significantly (P< 0.05). However, serum IL-8 was highest in GOLD stage IV and lowest in control significantly (P< 0.05). Over left groups were not ...
Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu*
...L-1β, IL-4 and IL-5 cytokines and increased the level of IFN-π in allergic asthma mice caused by OVA. In contrast, there was no significant effect in the body weight of mice treated with quercetin. Based on the result, we may infer that quercetin demonstrates an inflammatory mechanism for the therapeutic effect of inflammation in allergic asthma.
...
Ying Liu1, Shankun Liu1, Hui Wang2 and Weihua Su2,*
...um, lipids, antioxidant, cytokines and C-peptide was recorded. GDM group rats showed the lower weight of pregnant rats, fetal and placental weight as compared to other group rats. Caffeic acid treated group rats exhibited the modulated level of blood glucose level, lipid profile and antioxidant parameters at dose dependent manner. Caffeic acid significantly (P<0.001) enhanced the level of insulin, hepatic glycogen as compared to GDM control group rats. Caff...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...nter growth hormones and cytokines, STAT5A is activated to regulate gene transcription. STA5A has an important role in fertilization, embryonic survival and milk production traits in farm animals. The genomic characterization of STAT5A gene has not been assessed before in Nili Ravi Buffalo. In present research work, the sequence of the bovine STAT5A gene was analyzed to identify single nucleotide polymorphisms and its effect on evolutionary diver...
Abdelaziz A. Yassin, Ayman H. El Deeb, Abeer E. Mansour, Hussein A. Hussein
Zhengyi Fu1,2,3,4, Rui Yang1,2,3, Shengjie Zhou1,2,3, Zhenhua Ma1,2,3* and Gang Yu1,2,3*
...L grade pro-inflammatory cytokines were up-regulated, but anti-inflammatory cytokines were down-regulated and inflammatory markers were up-regulated, indicating an obvious inflammatory response. The expression levels of S and M grade anti-inflammatory and proinflammatory cytokines was more balanced. This study provides the basis for finding the relationship between size heterogeneity and g...
Liyun Chang1, Yingbin Chen1, Qian Kang1, JianhuaQin1,* and Zhiyong Liu2,*
...to the sequence of mouse cytokines in GenBank. The expression levels of the cytokines were normalized to GAPDH gene expression. Fluorescence threshold (Ct value) obtained from RT-QPCR2-ΔΔCt method with GAPDH standard curve (linearity R2=1), followed by sequencing analysis showed that all tested cytokines were amplified. The PCR products exhibited consisten...
Ou-Mei Hao1*, Xue-Feng Wang1, Zhi-Jun Yue1, Chun-Hong Nan1,Yan Yu1,Tong Zhang2,Yu-Feng Liu3 and Xue Zhao1
...and eosin. The levels of cytokines in lung tissues were assessed by Immunohistochemistry staining and Quantitative real-time (qRT)-PCR assays were performed to explore the effects of QZZKF on the expression of surfactant protein A (SP-A), B7 homolog 3 protein (B7-H3), interleukin (IL)-12 and IL-13. The expression levels of B7-H3 and IL-13 were upregulated after MPP infection in the mouse model (P<0.05), while the expressions of SP-A and IL-12 were significa...
Ayesha Muzamil, Hafiz Muhammad Tahir, Shaukat Ali, Iram Liaqat, Aamir Ali, Muhammad Summer
...o- and anti-inflammatory cytokines. The main component of immunity is IL-1. IL-6 cytokine play its role in regulation of metabolic reactions. The activity of various leukocytes is suppressed by IL-4 and IL-10 (anti-inflammatory cytokines) that triggers the production of pro-inflammatory cytokines. This review paper provides an overview of inflammation, its associated factors, causes and tr...
Jiao Ma*
...art lesions. The work of cytokines and growth factors has been found to play an important role in increasing endogenous cardiac stem cell proliferation and migration capacity. One of the successful approaches is to use these factors along with cardiac stem cells to boost the repair efficiency of heart injury. Therefore, in the present review study, the types of cardiac stem cells and their molecular phenotype, cardiac stem cell genetics and their ability to tr...
Doaa Sh. Mohamed1, Nema S. Shaban2 , Mai M. Labib3, Olfat Shehata4
Ameliorative Effect of GSPE Against AFB1 Induced Immunotoxicity and Hepatotoxicity in Japanese Quail
Maha Khalil1, Gamal Shams2, Hosny Abdel Fadil2, Nagah Edrees2, Mostafa Abonorag1, Nasser El-Sabbagh3, Eman A. Ahmed4*
...dation, pro-inflammatory cytokines, and histopathological investigations were assessed. GSPE supplementation alleviated AFB1-induced hepatotoxicity as reflected in diminishing alanine transaminase, aspartate aminotransferase, alkaline phosphatase, lipid peroxidation, and raising TNF-α and IL-6 as pro-inflammatory cytokines. Moreover, elevating the reduced levels of glutathione peroxidase, catalase, and superoxide dismu...
Asmaa Khamis1, Osama Abdalla1, Mohamed Hashem2, Noha Abdelnaeim1*
...ants, apoptotic markers, cytokines, and histopathological changes. Our results revealed a significantly increased MDA, caspase-3, p53, tumor necrosis factor-α, interleukin (IL)-1β, IL-18 and, IL-10, and a significant decline in glutathione and superoxide dismutase in the CCl4 group. However, intoxicated groups treated with PN and PM showed marked improvement in the measured parameters. Therefore, PN and PM have antioxidant and anti-inflammatory effe...
Xinjun Zhang1,2, Pengyong Wang1,2, Huimin Ju1,2, Yanhong Wang1,2, Yang Yang1,2* and Guoqiang Zhu1,2*
...hesis of proinflammatory cytokines were discussed. The results showed that fimH was 100% detected, followed by iut, iroN, and focA, with the detection rates of 71.4, 57.1 and 57.1% respectively; while papG allele I, papG allele I’, papG allele II, papG allele III, cdtI, sat, sfaS, iha and sat were not detected. Most of the isolated UPEC strains have a strong virulence on T24 cells and could induce strong immune response. Taking these data together, canin...
Hamdy Abdala Elnagar1, Wael Mohamed Wafa1*, Abdel-Khalek El-Sayed Abdel-Khalek2
Hairul Islam M. Ibrahim1,2, Abdalla A. Sayed1,3, Abdel Naser A. Ahmed4,5 and Emad A. Ahmed*1,6
...s. T-cell population and cytokines levels in myelin oligodendrocyte glyco-protein (MOG) challenged PCB treated cells were estimated using flow cytometry and ELISA kits. Apoptotic and neuronal markers were evaluated using quantitative real time PCR and protein blots. Results showed that PCB inhibited the infiltration of inflammatory cells and improved the myelin protective proteins. It also positively regulated the antioxidants and apoptotic markers including C...
Ahmed H. Abed1, Ahmed Orabi2*, Wafaa Hassan3, , Enas Hammad4 , Ghada Gaber5
Farah Abid1,2, Mohammad Saleem1,3*, Tahir Maqbool4, Tania Ahmed Shakoori5, Faheem Hadi4, Tahir Muhammad4, Saira Aftab4, Yasir Hassan7 and Shabana Akhtar4,6*
...sion levels of different cytokines and interleukins involved in inflammation. Real-Time PCR results showed a down-regulation of TNF-α, COX-2, IL-6, IL-1β, NF-κβ1and up-regulation of IL-4, IL-10 and I-κB genes. Levels of antioxidants, superoxide dismutase and catalase were determined to be significantly higher (p<0.05) in the group treated with E-AM compared to the arthritic group. In addition, histopathological examination indica...
Mohamed M. Bahr1, Mohamed S. Amer1*, Khaled Abo-EL-Sooud2, Shaimaa M. Kamel3, Marwa A. Fouly4, Ahmed N. Abdallah5, Ashraf A. Shamaa1, Omar S. El-Tookhy1
... involves the release of cytokines and nucleic acids (DNA, mRNA, and miRNA) which may lead to alteration of the gene expression, proliferation, and differentiation of the recipient cells. Methodology: 24 male New Zealand albino rabbits were used in this study, divided randomly into 4 groups (6 rabbits per group), induction of corneal ulcers centrally in groups (I, II) and peripherally in (III, IV). Treated groups are (I, III) using topical lyophilized canine ...
Xiaoying Liang1,2, Xiaofang Jiang3, Honglin Jia1, Ru Zhang1, Li Gao4* and Qing Yang5*
...T helper 1 (Th1) and Th2 cytokines by effector T cell polarisation. This study investigates changes in the marker genes of T cell subsets after allergen sensitisation and demethylation intervention as well as the T cells’ effects on allergic airway inflammation development. The number of T cell subsets and the expression of cytokines were detected by a well-established model of experimental asthma. The number of T cell...
Asmaa A. Darwish*, Adel M. El-Kattan, Mona A. Mahmoud, Mohamed T. Ragab, Amani A. Hafez
... in the pro-inflammatory cytokines, acute phase proteins (APPs), free radicals, cortisol, growth hormone, and TSH concentrations and a significant (P˂0.05) decline in the anti-inflammatory cytokine, anti-oxidants, insulin, T3, and T4 levels. The iron profile of infected animals was characterized by a significant (P˂0.05) hypoferremia, hyperferritinemia, and hypotransferrinemia. The estimated pro-inflammatory cytokines and ...
Lianci He1, Dingxi Bai2, Chenxi Wu2, Yizhu Zhong2, Shi Chen2, Xinru Bao2, Jing Gao2*, Chaoming Hou2*
...pression of inflammatory cytokines and protein targets, and has an immunoregulatory effect on chronic fatigue syndrome....
Weijun Chen1*, Shiyang Gao1, Ping Sun1, Xiaohui Sun2, Yongbin Wei1, Jingyang Chen1 and Jialong Chen1
...sion of pro-inflammatory cytokines (e.g., TNF-α, IL-1β and IL-6), increasing the activity levels of antioxidant enzymes, and upregulating the mRNA expression of Nrf2 and HO-1 in the liver (P<0.05). In conclusion, dietary NaBT supplementation could ameliorate the detrimental effects of high-fat diets on liver health by activating Nrf2 in common carp....
Mohammad Bani Ismail
...matory markers, and more cytokines. SPN treatment, however, reduced Dox-induced enlargement, attenuated hypertrophic markers, and attenuated the expression of inflammatory markers. Moreover, we saw increased levels of oxidative stress-related proteins NRF2, HO-1, and NQO-1. SPN protects against oxidative stress and inflammation-induced cardiomyopathies, according to our study....
Reza Esmaealzade Dizaji1, Arasb Dabbagh Moghaddam1*, Arash Ghalyanchi Langeroudi2, Mohamad Foad Heydari3, Seyyed Javad Hosseini Shokouh4 and Ana Shirzad Shahrivar5
...ory and pro-inflammatory cytokines. We found that the PRP/BNF group and the PRP group had faster wound healing rates than the model group at each time point in the current investigation. The same results were corroborated by the pathological score of skin wounds stained with H&E. Researchers found that in comparison to the control group, those in both the PRP/BNF and PRP groups had significantly lower expression levels of the mRNAs for interleukin 1, inter...
Sajida Batool, Iqra Ashraf, Shafaat Yar Khan, Sitara Shameem* and Breha Kazmi
...vels of pro-inflammatory cytokines (Interleukin-6 and Interleukin-8) were measured after 8, 12, and 24 h while levels of anti-inflammatory cytokines (Interleukin-10) were measured after 4 and 48-h via enzyme-linked immunosorbent assay (ELISA). C. procera treatment exhibited a significant decline in pro-inflammatory cytokines and an increase in anti-inflammatory cyt...
Asmaa A Darwish*
... in the pro-inflammatory cytokines, acute phase proteins, free radicals, globulin, triglycerides, kidney function tests, and liver enzymes concentrations and a significant (P<0.05) decrease in IL-10, antioxidants, total protein, albumin, glucose, total lipids, cholesterol, minerals, electrolytes, and trace elements concentrations. The DG hemogram clarified a significant (P<0.05) microcytic hypochromic anemia accompanied by neutrophilic leukocytosis and ...
Rana Mahmood Ahmad*, Orooba Mohammed Maeed Ibrahim
...are central inflammatory cytokines involved in the pathogenesis of chronic inflammation. Clove oil extract also increased levels of the anti-inflammatory cytokine IL-10, which is crucial in preventing tissue damage due to inflammation. These results led us to hypothesize that clove oil extract therapy speeds up the switch from an inflammatory to an anti-inflammatory response during the healing process. On the other hand, the positive control groups showed no i...
Qurrat ul Ain Shafique1, Sana Batool2, Hanfa Ashfaq2, Asima Tayyab2, Roquyya Gul3 and Mahjabeen Saleem1*
... cytokine and one of the cytokines that is approved by FDA for cancer immunotherapy particularly kidney and skin cancer. Interleukin-2 (IL-2) is a 4-helix-bundle type I cytokine possessing a cytokine receptor chain essential for the immune response. The current study presents a vector/host combination (pET28a(+)/IL-2) and optimization of expression in LB,TB and M9 media with varying concentrations of inducers (IPTG and lactose) and post induction time. Maximum...
Bushra AL-Khaqani, Amira Mohammed*
...(P<0.05) inflammatory cytokines including interleukin-17 (IL-17) and interferon-gamma (IFN-γ). RES increases (P<0.05) the expression of Il10 gene in the spleen, which is associated with T-regulatory and anti-inflammatory processes in the immune system. Our findings showed that RES may reduce asthma by activating anti-inflammatory genes, restoring lung barrier integrity, and decreasing proinflammatory cytokines an...
Afrah Adil Hassan1*, Hawraa F. H. Alowaid1, Majdy Faisal Majeed2
...y (MOP). Proinflammatory cytokines (TNF-α and IL-1β) were measured in the plasma samples, and an evaluation of the liver histological profile was conducted on liver samples. The results indicated that lead toxicity significantly (p≤0.05) increased MDA and MOP levels while decreasing GSH activity in the liver compared to the control group. However, this effect was significantly (p≤0.05) altered by the supplementation of Chy and Gnk. Similarly, ...
Naveed Hussain1*, Muhammad Arif Khan1, Asim Khalid Mahmood2, Muhammad Yasin Tipu3, Mamoona Chaudhry4, Hamad Bin Rashid1, Haroon Akbar5 and Abdul Aziz6
...e osteoprogenitor cells, cytokines and growth factors with appropriate tissue matrix resulting in osteogenesis, osteoinduction and osteoconduction. Orthobiologics are biological materials used to augment bone and soft tissue cure, with multifactorial effects. In our study, three orthobiologics i.e., Bone Marrow Aspirate (BMA), Platelet Rich Plasma (PRP) and Decellularized Fish Scale (DFS) were used in conjunction to autogenous bone graft. Objective was to tran...
Maysaa A. Jumaah*, Ali A. Al-Iedani
...2) and anti-inflammatory cytokines (IL-4 and IL-10) and innate immune responses (total white blood count, phagocytosis and respiratory burst). Our results demonstrated that all vaccines have induced both innate and adaptive immune responses. However, the adjuvant with alum hydroxide mixed with sonicated antigens and killed bacteria induced higher pro-inflammatory cytokine effects (IFN- γ P ˂ 0.05, P <0.0 1) and anti-inflammatory
Tanveer Hussain1, Abdul Wajid1, Jabbar Khan2, Asif Nadeem1, Misbah Hussain1, Qurat ul-Ain1 and Masroor Babar1*
...o and anti- inflammatory cytokines by activating several pathways. In present study, exon 1 of IL-2 gene of four local Pakistani breeds (Dera Din Panah, Beetal, Nachi and Kamori) was amplified by using reported ovine IL-2 primers. Amplified products of 4 breeds of goat were bidirectionally sequenced to decipher polymorphisms. Only a single substitution (T→A) was found in non-coding region of IL-2 gene. Comparison of IL-2 gene sequence of all four breeds w...
Shaza N. Alkhatib1, Rasha M. Ghoneim2, Hend M. Tag2,3, Hekmat M. Tantawy2, Heba M.A. Abdelrazek4*
Ashraf B. Said*, Ibrahim A. Ibrahim, Hoda I. Bahr, Marwa A. El-Beltagy
...levation in inflammatory cytokines with hepatic and kidney tissues disruption. At the same time, treatment alleviated hyperglycemia and IR with a significant (P≤0.05) decrease in inflammatory biomarkers levels and tissue expressions, especially GLP-1 and Stem cell groups combined with metformin. The study concluded that whether BM stem cells or GLP-1 able to decline hyperglycemia and inflammatory conditions associated with diabetes and have additive or even...
