Haq Aman Ullah1*, Aneela Zameer Durrani2, Muhammad Ijaz2 and Aqeel Javeed3
...ls. In the present study contamination status of aflatoxin B1 in cotton seed cake, wanda, wheat bran and homemade concentrate mixture of dairy goats was investigated in district Lahore of Pakistan. A total of Twenty 20 goat farms were randomly selected and out of that 40 feed samples 2 from each farm were collected in the month of April. All the samples were analysed for the determination of aflatoxin B1 by using reverse phase High Performance Liquid Chromatog...
Nazish Huma Khan*, Mohammad Nafees and Adila Bashir
... the soil and wheat crop contamination caused by heavy metals (Cd, Cr and Ni). Soil samples were collected from both orchard and non-orchard fields and were extracted with Aqua-regia and Mehlich-3 extracting solutions for total and available metals respectively. Wheat crops from both fields (orchard and non-orchard) were collected and analyzed with aqua-regia for heavy metals contents. Results showed that the average concentrations of metals in soils of orchar...
Zafar Khan, Asad Sultan, Rajwali Khan, Sarzamin Khan, Imranullah and Kamran Farid
...ign: justify;"> The contamination of the food stuffs by toxic metals is a threat and their exposure for long time has detrimental effects on the health status of animals. Chicken eggs are one of the main sources of protein but if contaminated by toxic heavy metals will cause a harmful effect on human health. The concentration of heavy metals (Pb, Cd, Cr, Fe, Mn and Zn) present in poultry egg and meat were determined in the following three districts; Pesha...
Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
...idences of antimicrobial contamination, especially in food, dairy products, agriculture and environment, their regular monitoring is on prime interest. Aptamers are synthetic short sequences of single stranded (ss) oligonucleotides (ss-RNA or DNA), which are developed by an in-vitro selection process known as “Systematic Evolution of Ligands by Exponential Enrichment (SELEX)” technique. Among the receptors available for biosensing, aptamer exhibit ...
Rashid Ahmed Khan* and Muhammed Naveed
...nmental pollution, fruit contamination and health hazards. The major goal of the present study was to evaluate the effectiveness of a bio-insecticide, emamectin benzoate with other conventionally used insecticides against B. zonata. The results revealed the high toxicity of emamectin benzoate with LC50 value of 38.25 followed by trichlorfon, λ-cyhalothrin and imidacloprid with LC50 values of 44.21, 58.98 and 187.81 ppm, after 24 h post treatment, respec...
Iram Liaqat1*, Najma Arshad2, Muhammad Arshad3, Safdar Ali Mirza4, Nazish Mazhar Ali1 and Ammara Shoukat1
...ign: justify;">Microbial contamination and biofilm formation in food industries pose a threat to human health worldwide. With the increased use of antibiotics in food industry, the problem of bacterial resistance is emerging, hence leading to serious health issues. Among 24 strains isolated from Shaukat Banaspati Ghee industry (Grw) and Shezan foods (pvt) Ltd, Lahore, Pakistan food industry, 10 highly antibiotic resistant strains were subjected to morphologica...
Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1
Nadeem Rashid1,*, Masroor Ahmad Bajwa1, Mohammad Masood Tariq1, Tanvir Ahmad2, Majed Rafeeq1, Zafar Ahmad1, Mohammad Arif Awan1, Mohammad Ali1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Asad Ulah1 and Amanullah Khan3
...yndromes to this type of contamination.
...
Wali Khan*, Ghazal Mumtaz, Saima Bibi and Salma Afzal
...e screened for parasitic contamination. The results showed that 10.7% of the vegetables were found to be contaminated with one or more than one species of parasites. Intestinal parasites Ascaris lumbricoides 26.7%, Trichuris trichura 19.6%, Taenia saginata 25 % and Entamoeba histolytica cyst 28.5% were detected. The highest prevalence was recorded for Entamoeba histolytica while Trichuris trichura was detected as the least parasitic infection. Coriandrum annum...
Hazrat Ali1,*, Wajid Ali1, Karim Ullah1, Fazle Akbar1, Sher Ahrar1, Irfan Ullah1, Irum Ahmad1, Ashfaq Ahmad1, Ikram Ilahi2 and Muhammad Anwar Sajad3
Fawad Ali1*, Hidayat Ullah2, Ikhtiar Khan1
...quality, leading to food contamination. The extractable concentration of metals viz Pb, Cr, Cd and Zn through ammonium bicarbonate diethylenetriaminpenta acetic acid (AB-DTPA) showed significant differences between the two studied localities. Linear regression analysis indicates that concentration of the heavy metal (Pb, Cr, Cd, Ni, Zn) in vegetables are significantly correlated at R2 value of 0.956 at P≤0.001 with their extractable concentration in soil. H...
Habibun Nabi1, Saher Islam2, Amna Arshad Bajwa2, Imran Rashid1,*, Haroon Akbar1, Wasim Shehzad2, Kamran Ashraf1, Nisar Ahmad1 and Aneela Durrani3
...d are the main source of contamination of soil and water. Fecal samples from cats at Pet center of UVAS (Lahore, Pakistan) were screened for coccidian parasites through microscopy examination. DNA was extracted from positive fecal samples for coccidian parasites and a T. gondii PCR was performed. Five sets of primers were designed using PrimerSelect tool for PCR to amplify SAG2 gene 5’ and 3’ regions. Sequences of 5 fragments of SAG2 were an...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...ed for avian mcyoplasmas contamination on cell lines or suspect fertile eggs for virus vaccine production.
... 
Ambreen Akhtar Saddozai and Samina Khalil*
... determine the microbial contamination in the most commonly consumed bakery products i.e., biscuit, cake, and bread. Samples were randomly collected from 12 sectors (main markets and sub-sector markets). These samples were analyzed for total plate count (TPC), total coliforms, fecal coliforms, E. coli, yeasts and moulds. None of the sample was found contaminated with E. coli, contamination of moulds was also negligible. TPC ...
Muneeb Zafar*, Tahir Mehmood**, Irfan Ahmad Baig**, Abdul Saboor** Mazher Abbas***, Shumaila Sadiq** and Khalid Mahmood****
ANALYTICAL DIAGNOSTICS OF NON-OPTIMAL USE OF PESTICIDES AND HEALTH HAZARDS FOR VEGETABLE PICKERS
...of pesticides results in contamination of environment (water, soil, and air) and increase the health cost of vegetable pickers. The purpose of this study is to estimate the excessive use of pesticides and economic cost of health hazards for the vegetable pickers in district Vehari. Data from 90 respondents were collected and analyzed. The most common health problems identified during the survey were headache, eye irritation, skin infection, cough and shortness...
Muhammad Jamal Khan1, Abdul Malik2*, Muhammad Shahzad Khattak2, Naveedullah1, Naeem Ijaz3 and Ishaq Ahmad4
...rld. In recent past, its contamination due to lack of balance between its exploitation and recharge has posed a serious menace on its utilization for various purposes. Current study was conducted in District Karak during June to November, 2009 with the objectives to assess the changes in groundwater table depths due to withdrawal and the water quality parameters and to find the effect of small dams on recharge of ground water aquifer. Water table depths data f...
Muhammad Umar1, Mubashar Hussain1,*, Ghulam Murtaza1, Farid Asif Shaheen2 , Fatima Zafar1
Muhammad Waheed Mushtaq1,*, Jamil Anwar2, Omara Naeem3,Farah Kanwal1 and Waheed-uz-Zaman1
... separation of bacterial contamination in aqueous systems. After complete freezing, the samples were collected from various sections of the frozen mass and bacterial colonies were counted after proper Gram staining. It was found that more than 90% bacteria were separated along with freezing front and were concentrated in the centre in the case of radial freezing and at the bottom in case of vertical freezing.
...
Abdul Samad1, Ferhat Abbas1, Zafar Ahmad1, Olena Pokryrshko2 and Tauseef M Asmat1,*
...tail market, the overall contamination of food samples with infectious agents was 48.37%. Campylobacter jejuni was recorded much higher (28.99%) compared to other foodborne pathogens. Food samples were also found positive for Salmonella spp. (19%), E. coli (O157:H7) (8%) and 1.25% for Listeria monocytogenes. 5% tested food samples were found co-infected with at least two pathogens. The results urge to adopt proper food hygien...
Nadeem Munawar, Iftikhar Hussain and Tariq Mahmood*
...y, by gnawing, spoilage, contamination and hoarding activities. The current study investigated the occurrence of different rodent species inside the crop fields and at field edges, associated with four major crops (wheat, groundnut, millet and maize) in the Pothwar Plateau. In all four crops, maximum number of rodent burrows was recorded at maturity stages of the crops. The maturity stage of wheat crop coincided with spring breeding season while the maturity s...
Ashraf M. Mounir, A.N. El shahat* and A.M. Abdul Azeem
...rradiation is used for decontamination of Boswellia tears to achieve satisfactory microbiological quality and public health safety. Hence, examining the effect of gamma-irradiation on the contents of Boswellia oleo-gum resin and also investigating the role of gamma-irradiate frankincense aqueous extract (GFAE) against ISO-induced MI in rats were the two aims of this study. The total phenolic content and total flavonoids of frankincense has been s...
Hamda Azmat1, Waqas Ali2, Arshad Javid2, Ali Hussain2, Syed Makhdoom Hussain3, Zara Saeed1,Syed Shafiq Hussain Bukhari4
Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan
Hina Ali*, Iram Shaheen and Feroza Hamid Wattoo
...l coliform and bacterial contamination. The water samples of Dhoke kashmirian, Pandora Satellite town, and Gulshan dadan khan indicated coliform, yeast and bacterial contamination which caused water-borne diseases such as Dysentry, Diarrhea and Typhoid fever. ...
Sakhra Mahmood1, M. Younus1, A. Aslam1, A.A. Anjum2, S. Umar3, Aamerzish Mushtaq3 and M.L. Sohail4,*
...having 0.5 mg/kg of AFB1 contamination and varying levels of SC (0.5, 1 and 2 mg/kg of feed). Another 120 birds were divided into two groups, one group was fed with basal diet (control group) and other was fed with AFB1 diet without any detoxifying agent. Growth parameters (body weight gain, feed intake, and feed conversion ratio), hematology (hemoglobin, haematocrit, erythrocyte sedimentation rate, and total leukocyte count) and immune response were recorded ...
Saleh S. Alhewairini1,* and Mahmoud M. Al-Azzazy1,2
...ts with minimal residual contamination.
...
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...
From last few years contamination of aquatic bodies has been increased due to extensive use of pesticides in agriculture. The exposure of aquatic animals to these pesticides results in acute and chronic health effects. Fresh water fish species such as Channa striata serve as important bio-indicators for aquatic contamination to access the changes caused by human activities effectively and reliable monitoring bio-...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
...studies as it eliminates contamination problem, remove batch to batch variation, long-lived and is readily available.
...
NaseemZahra1,2,*, Nadia Jamil1, Sajid Rashid Ahmad1, Imran Kalim2, MuhammadKhalid Saeed2, Ijaz Ahmed2, Qurat-ul-ain Syed2 and Soniya Munir1
...8% and 100% of aflatoxin contamination in branded and non-branded milk samples were found, respectively. The quantities of AFM1 in 58% of branded milk samples and 95% of non-branded milk samples were found beyond permissible limits of 50 ppt as set by European Union. The most pathetic condition of non-branded milk samples was found in Shalimar Town of Lahore which showed highest levels of aflatoxin M1 i.e. 2735.08 ppt.
...
Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed
...ects such as environment contamination. This laboratory study evaluated the toxicity of methanolic extracts of eight flowers i.e. Mexican marigold (Tagetes lucida), African marigold (Tegates erecta), tecoma (Tecoma stans), calendula (Calendula officinalis), basil (Ocimum basilicum), oxeye daisy (Leucanthemum vulgare), lily (Lilium longiflorum) and chrysanthemum (Glebionis segetum) against the worker individuals of subterranean termite Odontotermes obesus (Isop...
R. Yasmeen1*, S. Shaheen1, B.N. Khan2, S.S. Bokhari1, U. Rafi1 and A.W. Qurashi1
Tasbihullah1, Sadeeq ur Rahman2,*, Tariq Ali3 , Umer Saddique1, Shakoor Ahmad1, Muhammad Shafiq4, Sultan Ayaz2, Hamayun Khan1, Ijaz Ahmad1 Asadullah2, Raheela Taj5, Hafiz Nidaullah6 and Alamgir Khan7
...e current study indicate contamination of eggs with ESBL-producing E. coli suggesting to improve hygienic process for end consumer during- egg production.
... 
