Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
...nsferases (ALT and AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total protein, total bilirubin, hepatic glutathione (GSH), nitric oxide (NO), superoxide dismutase (SOD) and lipid peroxides (LP) levels were estimated. Moreover these biochemical parameters were confirmed by histopathological examination using hemotoxylin and eosin (H&E) and Mason trichrome stains (MTC). Immunohistochemical investigations for the expression of the proapoptot...
Abida Saleem, Sajida Parveen and Muhammad Jamal Khan
... potential in calcareous alkaline soils [Piedmont alluvium, silty clay loam, Ustochrept]. The Zn adsorption capacity was determined by adding 50 ml of 0, 5, 10, 20, 40, 60, 120, 240 and 360 mg Zn L-1 (initially Zn applied, IZnA) to 5 g soil with absence (non-treated) or to soil pretreated with FYM, PM or BC at rate of 10 g kg-1. The suspension was shaken on horizontal shaker for 20 h continuously to assess the equilibrium Zn concentration (EZnC). The differenc...
G.E. Odo1*, E.J. Agwu1, N.I. Ossai2, C.O. Ezea3, J. Madu1 and V. Eneje2
...se, reduced glutathione, alkaline phosphatase (ALP), alanine aminotransferase (AST) and aspartate aminotransferase (AST). Similarly, aluminium phosphide enhanced levels of AST, ALT and ALP as both concentration and time dependent in elevation were recorded when compared to the control. The use of aluminium phosphide in agricultural fields near water bodies should be strongly monitored in view of the observed effects on this catfish fish physiology. The use of ...
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
... aminotransferase (AST), alkaline phosphatase (ALP), blood urea nitrogen (BUN) and creatinine which indicate renal and liver damage. Concentration of Na, K, Ca, Mg, P, Cu and zinc in serum were significantly decreased (P< 0.05), indicating tubular damage in kidneys.
...
Mustafa Cengiz1*, Jama Hussein Ali2, H. Mehtap Kutlu2, Djanan Vejselova2 and Adnan Ayhanci3
...e transaminase (AST) and alkaline phosphatase (ALP)). In contrast, in the groups given D-GaIN and EA, a decrease in the damage of the liver tissue, a significant decrease activated Bax and caspase-3-positive hepatocytes, while increase in the number of Bcl-2 positive hepatocytes, a decrease in the biochemistry markers levels were found. Group 4, given EA before D-GaIN, showed better results when compared to Group 5, given EA after D-GaIN, in terms of histopath...
Rahat Naseer1*, Abu Saeed Hashmi1, Zulfiqar-ul-Hassan2, H. Rehman1, Saima Naveed1, F. Masood1 and M. Tayyab1
...g techniques using acid, alkali, water treatment and fermentation to prepare processed moiety on pilot scale. All these processed materials were included in experimental ration at a fixed level (20 %). All iso-caloric and iso-nitrogenous rations were offered to experimental animals (n=36, 22 male 14 female Lohi sheep almost evenly distributed in experimental groups) having almost uniform body weight (BW). During 60 days of the trial, no significant difference ...
Irfan1* , Arshad Javid2 , Muhammad Altaf3 , Muhammad Shahbaz3 , and Khalid Javed Iqbal4
...ed for adult birds while alkaline phosphatase (ALP) values were significantly (P<0.05) higher 104.86±16.39 iu/L in grower birds. Similarly, the rearing systems also influenced biochemical parameters of M. gallopavo and significantly (P<0.05) higher values for cholesterol 6.18±0.07mmol/L and ALT 31.26±1.67 iu/L were recorded in birds reared under confinement rearing system. Values for globulin 1.92±0.36g/dL and creatinin 0.77&p...
Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1
Farah Rauf Shakoori, Momina Shokat, Faiza Saleem and Tanzeela Riaz
...ated temperatures and in alkaline conditions. The present study was aimed to isolate and screen xylanase producing bacteria and to optimize cultural conditions for maximum production of enzyme from locally isolated bacteria and to optimize the assay conditions under which enzyme possessed maximum activity. Two bacterial isolates XPB-GS02 and XPB-CW01 with maximum activity ratio showed 18.3 and 12.2 U/ml xylanase activity, respectively. Maximum xylanase was pro...
Imran Khan*, Zahir Shah, Wiqar Ahmad, Farmanullah Khan and Muhammad Sharif
Attia Anwar1, Sajida Malik2*, Zobia Usman1 and Sadia Chiragh3
...d platelet counts, SGPT, alkaline phosphatse, serum uric acid, blood urea and serum creatinine. All the numerical data were expressed as mean± SD. The values in two groups were analyzed with help of unpaired t-test. A p-value ˂ 0.05 was considered significant. After 24 weeks of treatment with berberine hydrochloride, no significant differences were observed in Hb%, platelet count, liver enzymes and RFTs between two groups. There was a significant i...
Muhammad Kashif Maan1, Muhammad Arif Khan1, Shehla Gul Bokhari1, Muhammad Ijaz2, Hamid Akbar1, Sajid Umar 3 and Muhammad Luqman Sohail4,*
...t;0.05) was observed for alkaline phosphatase, creatinine and bilirubin between the two groups. Heart rate, blood pressure and saturation of oxygen were decreased in both groups but this decline was significantly lesser (P<0.05) in isoflurane administered group compared to xylazine-ketamine administered group. Both xylazine-ketamine and isoflurane proved to be suitable anesthetic agents for successful ovariohysterectomy in rabbits. As injectable anesthesia ...
Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**
BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...a-amylase, glucoamylase, alkaline phosphatase (AkP), acidic phosphatase (AcP), total protein, soluble protein and free amino acids (FAA). The sixth instar larvae of T. castaneum were released and exposed for 48h without food on abamectin treated glass petri dishes. The surviving ones were then homogenized in saline and centrifuged prior to biochemical analyses. Results showed differences in the activities of enzymes and quantities of total protein, soluble pro...
Zhiping Hu, Hussain Ahmad, Jingfei Zhang, Lili Zhang and Tian Wang*
... The results showed that alkaline phosphatase in T2 group was significantly decreased at 21d, blood urea nitrogen and alanine aminotransferase were significantly increased compared to CON group at 42d in broilers. Serum globulin content (21d) and total protein content (42d) were lower in T2 group than that of CON group. Serum total antioxidant capacity was higher and malondialdehyde content was lower in T1 group than that of CON group at 21d. Serum glutathione...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto2
...ne enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidase, and trypsin. While, esterase, esterase lipase, α-chymotrypsine, α-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, N-acetyl- β-glucosaminidase, α-mannosidase, and α-fucosidase were recorded as negative. Moreover,
Imran Khan1, Zahir Shah2, Wiqar Ahmad3*, Farmanullah Khan2 and Muhammad Sharif2
...cessary, deep tillage in alkaline calcareous soil should be avoided, preventing lime resurfacing from depth and keeping the applied amendments concentrated in surface layer. ...
Fazul Nabi1,2, Hui Zhang1, Muhammad Kashif Iqbal1 , Mujeeb ur Rehman1, Muhammad Shahzad3, Khalid Mehmood1,3 and Jiakui Li1,*
...ed lameness, decrease in alkaline phosphatase (ALP) activity and increase in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in the serum along with decrease in level of antioxidant enzymes and significantly increase in the MDA contents in TD afflicted chickens compared to control group. The SM administration to TD affected birds significantly ameliorated lameness, stimulated ALP level with a decrease in ALT and AST contents, inc...
Abdur Rehman and Zahir Shah
...ivum L.] under silt-loam alkaline soil conditions. Treatments were arranged in randomized complete block design (RCBD) with three replications. The required Fe and Mo as [(NH4)6Mo7O244H2O] and FeSO4.7H2O respectively were applied to soil followed by thorough mixing before sowing. Both Fe and Mo induced significant increases in fresh pods pea yield and nutrient uptake. Their combined effect was more prominent than their alone application. The maximum fresh pods...
S. Jalbani1, P. Khan2 and N.T.Narejo2,*and Y.M. Jalbnai3
....60, DO 3.9- 4.9 (mg/L), alkalinity 155-175 (mg/L), ammonia 0.37-0.52 (mg/L) and nitrate 0.168-0.174 (mg/L) were found to provide optimum condition to the fish.
...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
Hareem Mohsin, Azka Asif*, Warda Fatima, Sarooj Nadeem, Anjum Nasim Sabri
Assessment of biocalcification by the water absorption test using microorganism from oil contaminated site
Tahir Abbas*1, Khawaja Raees Ahmad2, Asmatullah3, Khalid Pervaiz Lone4, Muhammad Ali Kanwal2, Sadia Suleman2
Reno-hepatic protective effects of Jambul against chromium induced anomalies in mice
Babar Tasim1, Tariq Masood1*, Zafar Ali Shah1, Muhammad Arif2, Ata Ullah1, Ghazal Miraj3 and Muhammad Samiullah4
...on. All the samples were alkaline in nature with substantial values for electrical conductivity. Macro and micronutrients such as Zn, Mn, Mg and P were high (19.6, 20.4, 37.31 and 237 mg L-1, 510 mg 100g-1 respectively) in AD biochar while minimum values for Mn, Cu, P and Na (12.0, 20.4 mg L-1, 150 and 38 mg 100g-1) were noticed in SS biochar samples. All the biochar samples were highly porous except AD and MC biochar samples. The most abundant functional grou...
Hina Ali*, Iram Shaheen and Feroza Hamid Wattoo
Saleha Gul1,2, Muhammad Khisroon2, Ajmal Khan2, Attaullah1, Saira Gul3 and Gul Nabi Khan1,4,*
Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1*
...pH increased towards the alkaline side and recorded pH = 9 till day 33 of the experiment. Regarding moisture %, after mixing the manure with allantoic fluid the moisture % of the mixture was 67%, and this wet manure remained moist (67%) till day 4; due to the humid environmental conditions applied at room temperature. Later, the moisture content of the treated manure decreased gradually and recorded 52.5%, 40% and 32% at the 8th, 28th and 33rd days of experime...
Yuyu Wang, Gangchun Xu*, Zhijuan Nie, Quanjie Li, Nailin Shao and Pao Xu*
... aminotransferase (AST), alkaline phosphatase (ALP), superoxide dismutase (SOD), cortisol and lysozyme in serum showed no significant differences between the two stocking groups (P>0.05). Fish reared at high stocking density had significant lower total protein (TP), cholesterol (TC), triglyceride (TG) and glucose (Glu) content in serum compared with those reared at low density on day 90 and 120 (P<0.05). In conclusion, the present results i...
Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain1
...d to isolate cold active alkaline protease and alkaline active lipase producing psychrotrophic bacteria from water and soil samples collected from different glaciers of Karakorum Range of mountains, Pakistan. Serial dilution and plating approaches were exploited for bacterial isolation and the isolates were qualitatively screened for proteolytic and lipolytic activity with skim milk and Tributyrin agar (TBA plate) assay meth...
Munazza Yousra1*, Sair Sarwar1, Muhammad Mahmood Ul Hassan1, Muhammad Zameer khan1, Abdul khaliq2, Shahbaz Ahmad1 and Shamim Ul Sibtain Shah3
Constance Obiageli Ejilibe1, Helen O. Nwamba2, Ifeanyi Chinedu Atama3,*, Chiamaka Lynda Ani2, Ifeanyi Oscar Aguzie3, Josephine C. Madu3 and Christopher Didigwu Nwani3
... aminotransferase (AST), alkaline phosphatase (ALP) and total protein (TP) concentrations in homogenized muscle samples was determined by standard procedures. ALT, AST and ALP increased significantly in response to concentrations of Butaforce® (7.00, 9.00 and 11.00 µgL-1) and Termex® (15.00, 20.00 and 25.00 µgL-1) used. The TP decreased in response to the same concentrations of pesticides. The res...
Zubaria Malik, Zahir Shah* and Muhammad Tariq
...adiata)} in a calcareous alkaline soil of Peshawar valley during 2015/16 and 2016/17. This study was conducted in an already established experiment which was in a randomized complete block design with split plot settings. Keeping cropping systems in main plots and biochar levels in subplots. Biochar treatments were no biochar (T1), 20 t biochar ha-1 in each season (T2), 40 t biochar ha-1 in winter (T3) and 40 t biochar ha-1 in summer season (T4). Root samples ...
Uzma Rafi*, Asmat, Muqaddes Niaz, Syeda Shazia Bokhari and Aisha Waheed Qurashi*
S. Jalbani1, N.T. Narejo2,* and P. Khan2
...gen; ammonia nitrate and alkalinity were monitored fortnightly and found within the suitable ranges. Finally decided that chopped chicken viscera found to be ideal food for better growth and survival of catfish Rita rita reared in cemented cisterns....
Min Li1,2, Jingjing Wang3, Suxian Hu3, Philip Stott4, Baoqing Lin5, Lianshan Li5, Hui Liu1, Heng Bao1, Duoying Cui6 and Guangshun Jiang1,*
...pen water, farmland, and alkaline marsh, and negatively by human settlement; and in addition, these relationships were only apparent when scale was considered. We detected the dependence of the avian assemblage on alkaline marshes and open water, which in turn might depend on incoming flows of water. We also found negative relationship between human settlements and bird diversity which extended to a distance of 3 km. Consequ...
Tanzeela Riaz, Fiza Sana-Ullah Khan, Farah Rauf Shakoori
...eir high stability under alkaline and acidic conditions. The current research was planned to isolate, screen and optimize mannanase production from Bacillus megaterium from decayed fruits. Microorganisms were isolated from fruits, soil and water samples obtained from different localities of Lahore city, Pakistan. Locally isolated microorganisms were screened for their mannanase producing characteristics on mineral salt agar medium pH 7. Six isolates wer...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
...inotransferase (ALT) and alkaline phosphatase (ALP) levels. Aflatoxin M1 (AFM1) was detected in all milk samples of the Group B, C, and D in concentration higher than 0.05 ppb. The AFB1 was excreted in milk as metabolite AFM1 @ 1.35-1.59%. Udder health and milk quality deteriorated as SCC and TVC increased. Levels of serum enzymes AST and ALT increased with ingestion of dietary AFB1. It is concluded that ingestion of very low level of AFB1 by lactating goats r...
Chaman Ara1*, Asmatullah1, Sehrish Kanwa1, Asma Chaudhary2, Ayesha Siddiqua1
...rease in levels of total alkaline aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and bilirubin was observed in blood plasma. Urea level showed a remarkable decrease, whereas creatinine level increased but not significantly. Histological examinations exhibited pyknosis, necrosis, vacuolations, increased sinusoidal spaces, karyomegaly, glomerulosclerosis, glomerulonephritis, epithelium degener...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...tate transaminase (AST), alkaline phosphatase (ALP) and a significant increase in bilirubin levels in BP group. Significant raised patterns were recorded for urea and creatinine in BP intoxicated mice against control. Histological defects observed were necrosis, pyknosis, increased sinosuidal spaces, portal hypertension, glomerulosclerosis, abnormal vascular pole in liver and kidney respectively. But when turmeric powder was coadministered with BP, these damag...
Hafrijal Syandri1*, Ainul Mardiah2, Azrita3 and Netti Aryani4
...6 mg/L, pH 6.56 to 6.96, alkalinity 50.65 to 52.25 mg/L, and hardness 6.65 to 66.85 mg/L. Survival was high at 100.0% in synthetic sheet pond at 10, 15 and 20 fish/m3, respectively. Density significantly affected (p<0.05) final mean weight (164.72±2.20, 155.34±1.40, 138.40±0.98 g) and percent weight gain (113.04±5.10, 100.89±3.41, 78.98±2.63%), specific growth rate (0.84±0.03, 0.78±0.02, ...
Tong Feng, Zilu Zhang, Minghao Qu, Chan Luo, Laiba Shafique, Qingyou Liu and Kuiqing Cui*
...of 8.70, which is weakly alkaline, and contains seven transmembrane domains typical of cell membrane receptor proteins. Sequencing analysis of the black, brown and white different color MC1R genes of Nubian goats revealed that there are three SNPs in the gene sequence, which are 219, 712 and 1160, respectively. The C/T mutation did not cause amino acid mutations; base A/G mutations occurred at position 712 and caused amino acid mutations in pure black and brow...
Muhammad Asif Gondal, Qazal Waheed, Sana Tariq, Waseem Haider, Aisha Khan, Qudsia Rasib and Haroon Ahmed*
... presence of snails e.g. alkalinity of the soil indicates the survival of more number of species. A significant correlation was found between diversity and equitability (Pearson correlation value=0.961, p-value=0.00). Higher the electrical conductivity of soil lower is the diversity of snail. A significant correlation was found between diversity and equitability (Pearson correlation value=0.961, p-value=0.00). Electrical conductivity and pH showe...
Sana Aslam1, Wayne Thomas Shier2 and Imran Sajid1*
...wed an optimum growth at alkaline pH (9.0-9.5). The taxonomic analysis revealed that these isolates were closely affiliated with the genus Streptomyces (62.5%), Lentzae (6.25%), Pseudonocardia (6.25%), Lechevalieria (6.25%) and Nocardiopsis (12.5%). Most of the isolated strains exhibited strong inhibition against a broad range of pathogenic test organisms. The partial purification and metabolite profiling using ultra performa...
Boxin Dou, Ying Liu*, Yumeng Liu, Lili Fan, Yongqiang Ma, and Yanguo Shi
...es was obtained by using alkaline protease to enzymatic hydrolysis of RB crude protein purified by ultrafiltration, size exclusion chromatography and RP-HPLC. Using consecutive chromatographic techniques successively, the acidic hydrolysates of RB proteins were fractionated and the new ACE inhibitory triplet was isolated and identified. The amino acid sequence of the ACEI was identified as Ile-Thr-Leu or Leu-Thr-Ile. In vitro, ACE inhibition assays show...
Bin Huo1 and Xiaoyun Shen1,2,3*
...P<0.01). Serum alkaline phosphatase values from affected Tibetan gazelles were significantly higher than those from healthy ones (P<0.01). Serum inorganic phosphorus contents of affected Tibetan gazelles were about half of those in healthy animals. Oral supplementation of disodium hydrogen phosphate in affected Tibetan gazelles successfully treated the illness. This study demonstrated that the ailment of the Tibetan gazelle was mainly cause...
Mahmood Alam Khan1, Muhammad Yaseen1, Mujahid Khan*2, Amjad Khan1
TEMPORAL VARIATION IN GROUND WATER QUALITY DUE TO URBANIZATION. CASE STUDY: DISTRICT PESHAWAR, PAKISTAN
... (TDS), turbidity, total alkalinity, total hardness,
calcium (Ca++), HCO3-1 and Magnesium (Mg++) were decreased by 6%, 10.51%, 37.39% and 11.52%, 22.21%,
37.56% and 21.19% respectively. Results revealed that changes in water quality have occurred due to over population
of Peshawar and remedial measures are needed in future....
calcium (Ca++), HCO3-1 and Magnesium (Mg++) were decreased by 6%, 10.51%, 37.39% and 11.52%, 22.21%,
37.56% and 21.19% respectively. Results revealed that changes in water quality have occurred due to over population
of Peshawar and remedial measures are needed in future....
Zahid Hassan Tarar1, Muhammad Salik Ali Khan2*, Shahzada Munawar Mehdi3, Raza Salim4, Irfan Ahmad Saleem1, Saima Nazar5, Munir David2, Tahir Majeed6, Muhammad Sufyan Mughal7, Muhammad Saleem8, Umer Iqbal9, Muhammad Mazhar Iqbal10 and Muhammad Khalid Shaheen3
...onutrients deficiency in alkaline-calcareous soils is a major constraint to get optimum crop yield. The possible solution could be the site-specific nutrient management after assessing the micronutrient status at a particular site. This study was conducted to quantify and visualize the spread of plant available micronutrients in district Mandi Bahauddin. Total 1194 georeferenced soil samples were collected at a depth of 0-15cm maintaining a sampling grid cell ...
Shikun Ge1, 2, Yanli Yang3, Brindha Chelliappan4, 5, Antonysamy Michael5, Fagang Zhong1 and Xiaoying Zhang1, 2, 3, 4*
...ostability, IgY acid and alkali resistance, resistance to trypsin and pepsin, four different IgY purification methods (PEG extraction, water dilution method, the effect of liquid egg yolk and egg yolk powder preparations) on the activity of IgY antibodies was investigated. The results showed that IgY was stable below 70 oC, and the activity was significantly lost when it exceeded 70 °C; IgY was relatively stable in the range of pH 4-10, and the ...
Hafiz Hammad Ahmad1, Mukkram Ali Tahir1*, Noor-us-Sabah1, Ghulam Sarwar1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1 and Aneela Riaz4
Growth and Yield Response of Sunflower to Organic Amendments in Aridisol
...mical characteristics of alkaline-calcareous soils make P the least available macronutrient. Therefore, alternative P source such as rock phosphate (RP) can offer a promising choice for sustainable P supply under P stress environment. A pot research was executed to explore the response of sunflower (Helianthus annuus L.) to sparingly soluble P sources grown under P deficient environment at research area, College of Agriculture, University of Sargodha during 20...
Tasneem Shah*, Muhammad Tariq and Dost Muhammad
Chaman Ara1*, Asmatullah1, Saima Hanif1, Shagufta Andleeb2, Beenish Zahid3 and Madeeha Arshad4
...tate transaminase (AST), alkaline phosphatase (ALP) in liver and urea, and creatinine levels in kidneys, in Al exposed groups against control. In Al + antidote groups these deviations were less significant (P≤0.05). However, these morphometric and biochemical alterations improved to a great extent when onion extract was co-administered with Al. So it is concluded that Al caused morphological and biochemical disturbances in mice, whereas onion extract showed...
Rahat Rukhsana1, Shahnaz Rashid2, Asma Fatima2, Owais Iqbal Khan3, Syed Babar Hussain Shah4, Mumtaz Ali4 and Ghulam Abbas2*
...for contents of ammonia, alkalinity, temperature, nitrate, salinity, nitrite, pH, total hardness, dissolved oxygen (DO), chlorophyll-a and phytoplankton-density during the period from March, 2015 to June, 2016. The chlorophyll-a concentration was determined by using the acetone extraction technique. Ponds water sample (50 liters) was filtered through plankton net of 20 µm pore size and preserved in 4% formaldehyde for the analysis of phytoplankton. Subse...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
...pression times using the alkaline phosphatase labeled N-terminal anti histidine monoclonal antibodies revealed single bands at the expected size, the same samples have been reacted with serum collected from aborted and infected mares revealed band at the expected site using anti equine IgG labeled horse radish peroxidase, concluding that the expressed envelope glycoprotein D of EHV-1 (EHV-1gD) could be used as a good candidate for manufacturing of diagnostic A...
A. A. Rezk1,3, K. A. Alhudaib2 and A. M. Soliman2,3
...rified and conjugated to alkaline phosphatase. The conjugated antiserum was evaluated by indirect enzyme-linked immunosorbent assay (ELISA). The conjugated antisera that prepared in this study were succeeded to detect the virus in infected plants....
Jia Li and Hong Yang*
...alkyl polyglucosides and alkaline treatment during the waste activated sludge (WAS) anaerobic fermentation. During the experimental study, the biodegradation efficiency of BaA was enhanced from 12.2% in the control to 25.6% at pH 10 and 46.7% at pH 10 and alkyl polyglucosides (APG) reactors. APG and alkaline treatment increased the BaA deposition desorption from the sludge and transfer into micro-organisms and finally increa...
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
... aspartate transaminase, alkaline phosphatase and creatinine and a significant decline in alanine transaminase, total protein, total albumin, globulin, triglycerides and cholesterol values. Histopathological examination demonstrated degenerative changes, necrosis haemorrhages, and sloughing off epithelial cells of broad folds of caeca, mild lymphoplasmacytic infiltration in the periportal area of the liver and mild depletion of lymphocyte in the bursa of Fa...
Kirshan Chand1, Fahad Nazir Khoso1*, Arfan Ahmed Gilal1, Abdul Mubeen Lodhi2, Agha Mushtaque Ahmed1, Ghulam Murtaza Jamro3, Sohail Ahmed Otho1 and Jamal-U-Din Hajano4
... water samples (38) were alkaline in nature and unfit for dilution purposes of insecticide including Abamectin, Cyromazine, Fluvalinate, Imidacloprid, Methiocarb and Spiromesifen. Moreover, all the samples were exceeding the permissible pH level of 4.0 to 6.5 required for the mixing of commonly used insecticide such as Acephate, Azadiachtin, Buprofezin, Fenpropathrin, Fenpyroximate, Flonicamid and Pyriproxyfen. Furthermore, there were 23 samples (58%) exceedin...
Syed Shahid Imran Bukhari1*, Nusrat Jahan2, Muhammad Khalil Ahmed Khan3, Mariam Zaheer1, Sabir Javed1
...(127.67±4.67) and alkaline phosphatase (86.33±1.86) compared to their corresponding control group values. A. barbadensis (400mg/kg) reduced cholesterol (78.00±2.08), triglycerides (83.67±3.28), HDL cholesterol (49.33.67±2.33) and LDL cholesterol (61.67±3.84). Pancreatic and hepatic sections from diabetic induced group 2 mice showed vacuolization and inflammation of cells whereas, group 3 showed improvements with normal...
Najeeb Ullah1*, Muhammad Nadeem2, Malik Mujaddad-ur-Rehman1, Rubina Nelofer2 and Yasir Arfat3
... potential substrate for alkaline protease production at optimized conditions by Bacillus sp.
Novelty Statement | In present research work industrial skin waste obtained from the local leather industry was exploited as substrate for alkaline protease production that make the process economically feasible and environment friendly by utilizing the industry waste...
Abdullah Abdo Albegali1, Tayyaba Aftab1, Atiq-ur-Rehman1*, Amir Rashid1 and Mayada Mohammad1,2
...ate aminotransferase and alkaline aminotransferase. The plant maintains the level of HDL to a normal range. It is concluded from the study that the plant may be supportive treatment in combating hyperlipidaemias.
...
A.H. Alqhtani1, A.S. Alharthi1, N.J. Siddiqi2, S. Zargar2 and A.M. Abudabos1*
...yl transferase (GGT) and alkaline phosphatase (ALP) showed no significant differences between the groups. Thus, it can be concluded that probiotic, prebiotic and symbiotic in feed may cause some degree of liver damage as indicated by the release of AST and ALT in the serum....
Kanakuntla Sandhyarani*, Dhoppalapudi Madhuri, Yadala Ravikumar
... aminotransferase (AST), alkaline phosphatases (ALP), serum total proteins, serum albumin and glutathione stimulating hormone (GSH). Grossly, chalky white deposits of urate crystals on the serosal surface of pericardium, liver, intestines, air sacs, kidneys and ureters. Microscopically, tissue sections show urate crystal deposition in parenchyma of the organs along with infiltration of inflammatory cells. Incorporation of low protein diets, jaggery mixed water...
Heba El-Zahar*, Zeinab Abd El-Rahman, Abbas El-Naggar
...d creatinine, lipase and alkaline phosphatase (p<0.01) in IBD dogs. The mean values of IBD-related biomarkers (CRP, haptoglobin, and fecal calprotectin concentrations) increased significantly (p<0.05 or p<0.01) compared to controls. CRP, haptoglobin concentrations, and fecal calprotectin were found to have a strong positive correlation. Furthermore, blood enzymes, glucose and electrolytes were significantly correlated with CRP as an IBD-related biomar...
Ameliorative Effect of GSPE Against AFB1 Induced Immunotoxicity and Hepatotoxicity in Japanese Quail
Maha Khalil1, Gamal Shams2, Hosny Abdel Fadil2, Nagah Edrees2, Mostafa Abonorag1, Nasser El-Sabbagh3, Eman A. Ahmed4*
...artate aminotransferase, alkaline phosphatase, lipid peroxidation, and raising TNF-α and IL-6 as pro-inflammatory cytokines. Moreover, elevating the reduced levels of glutathione peroxidase, catalase, and superoxide dismutase were reported. Conclusively, the results of our experiment suggest that that GSPE is a good feed additive for alleviating the negative impacts induced by aflatoxin B1.
Keywords | Aflatoxin B1, Hepatoto...
Barirah Rehman Talpur1, Zaheer Ahmed Nizamani1*, Imdad Hussain Leghari2, Mansoor Tariq1, Aisha Rehman3, Shahnawaz Kumbhar1
Ahmed Mohamed Elmahdy1*, Naglaa Mohammed Alkalamawy2
... aminotransferase (ALT), alkaline phosphatase (ALP), g-Glutamyl transferase (GGT) and total bilirubin, also the levels of Cholesterol, Triglycerides, HDL-cholesterol, LDL- cholesterol and VLDL-cholesterol were also estimated. The degree of renal protection was measured using creatinine, urea, sodium, potassium, also the levels of total protein, Albumin and Globulin were quantitated also the histo-pathological examination of the liver, kidneys were done. Result...
Shambel Boki Dabi
...ch as CO2, Chloride, and alkalinity were instantly assessed using the titration method, while water turbidity, temperature, water pH, and dissolved oxygen were measured directly on site. A simple test kit was used to evaluate nitrite and ammonia levels. The result revealed a high level of alkalinity (66.3±39. 1mg. L-1) at downstream and lowest concentration (58±40mg.L-1) was found in the middle stream. The uppe...
Asma Fatima*, Ghulam Abbas and Shahnaz Rashid
...otassium, calcium, total alkalinity and total hardness were determined. Overall, 61 species of plankton have been recorded from the studied ponds and categorized into 19 groups; of which 25 phytoplankton species belonged to four major groups, whereas, 36 zooplankton species belonged to 15 groups. Among them, Bacillariophyta (69.815%) and copepods (78.927%) were “most abundant” as compared to other groups of phytoplankton and zooplankton, respective...
Mohammed A. Almujtaba1, Turky Omar Asar2, Salma Naqvi3, Vikas Kumar4, Fahad A. Al-Abbasi1, Abdulbasit I. Al-Sieni1 and Firoz Anwar1*
... Studies report that its alkaline nature renders it a therapeutic potential. We aimed to determine the effect of Zamzam water in doxorubicin induced cardiac remodeling in rats. Male Wistar albino rats were divided into 4 groups (n=6) for 28 days of experimental protocol. Group-1 served as normal control (NC), group 2 as doxorubicin control (DC), group 3 as doxorubicin+ Zamzam treatment (DZ) and group 4 as Zamzam control (ZZ). Doxorubicin (1mg/ kg bw) was injec...
Shujaat Hussain1*, Muhammad Saqib1, Khurram Ashfaq1 and Zia ud Din Sindhu2
...±0.65 µ/L), alkaline phosphatase (94.10±0.89 µ/L) and calcium (9.48±0.39mg/dl). On the other hand, a significant decline (p<0.05) in WBC count (11.94±0.04), eosinophils% (3.10±0.20), basophils% (0.76±0.06), platelets count (103.2±0.56103µ/l), glucose (55.13±0.84mg/dl), albumin (4.25±0.01g/dl) and sodium (110.26±1.21mEq/L) were observed in infected camels. This investigat...
Muhammad Hanif Chandio1,2*, Naeem Tariq Narejo2, Muhammad Farooq Hassan3, Faheem Sadar4, Nasiruddin Shaikh1, Bushra Ainy Dars5, Ghulam Abass6, Asma Fatima6 and Shahnaz Rashid6
...oxygen (4.0 to 4.8mg/l), alkalinity (149 to 180mh/l), nitrate (0.17 to 0.18mg/l) and ammonia from 0.35 to 0.55ug/dLumol/litre. Theresults of the current study concluded that Channa punctatus have a preference to living in a small community than large schooling and could be reared 10/fish/m2 was found to be suitable density for growth and survival in cemented cisterns. Present study plays an important role for culturing and rearing of native snakehead species.<...
Zaidi Zona, Zulfiqar Ali*, Rida Ahmad and Irfan Zainab
...tal hardness 77.50 mg/L, alkalinity 72.17 mg/L (as CaCO3) and conductivity 129.04 µs/cm. Planktonic study revealed the presence of variety of phytoplankton (n=31) and zooplankton (n=3) species in study area. In total, 37 fish species were found in the study area, with species richness rising from zone one (n =10) to zone five (n =33). Altogether, the fish diversity represents 25 genera and 13 families. To analyze the data, principal component analysis (P...
Emtiaz Ibrahim Ghoniem, Gaber Mamdouh Abdelgalil, Amira Farouk Gad* and El-Sayed Hassan Eshra
...ylcholinesterase (AChE), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in T. pisana intoxicated with two sublethal doses of CuSO4 (0.25 and 0.5 of LD50) after 24, 48 and 72 h were examined. The results indicated that the LD50 values of CuSO4 were 166.5, 92.59 and 70.63 µg/g b.w for 24, 48 and 72 h, respectively. The biochemical effects of CuSO4 led to a significant increment of AChE activities ...
Ghazala Nasreen1, Ali Hussain2, Komal Tayyab1, Muhammad Rashid3 and Sumaira Aslam1*
...smn; 0.23 IU L−1), alkaline phosphatase (ALP, 81.50 ± 1.49 IU L−1), aspartate aminotransferase (AST, 30.25 ± 1.62 IU L−1) and superoxide dismutase (SOD, 19.75 ± 1.78 IU L−1) were recorded in the liver samples of the fingerlings of the treatment group T1, while the lowest corresponding figures were noted for the fingerlings of the treatment group T4. These values were lower than found in the control group having 10.5...
Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2
...oduced from the acid and alkaline pretreated samples of sugarcane bagasse using a fermentation process. Amylase was partially purified from the bacterial strain N1 and its activity was checked at distinct pH and temperature. The optimum pH was from 6-8 whereas the temperature was 40 °C. Saccharomyces cerevisiea was added for enhanced fermentation and ethanol production. The acid pretreated sample produced the highest ethanol (15.2%) than the
Bashir Ahmad1*, Ali Muhammad Yousafzai2, Waqar Ali1, Ikram Ilahi1, Farman Ullah3, Saeed Ahmad1, Ayaz Ali Khan1, Umair Ahmad2 and Hafsa Maria2
... the high serum rates of alkaline phosphatase (ALP), alanine transaminase (ALT), and aspartate transaminase (AST). The outcomes of the extract-treated rabbits were comparable to those of the group of rabbits given silymarine. RBC, platelets, haemoglobin, MCH concentration, and HCT levels did not significantly alter after treatment with garlic aqueous extract and silymarine. However, a substantial (P 0.05) rise in the overall WBC count was seen. The current res...
A. A- E. Abo-Elkhier1, K. S. E. Abdel-Wahab2, M. A. Ali3, M. A-H. El-Sayed4, N.A-K. Saleh5 and A. A. Atef6
...Except total protein and alkaline phosphatase). It was found that 3 anti-HEV IgM. anti-HEV IgG. HEV RNA and HEV Ag positive pregnant women transmitted the virus to their all fetus (100%). While 14 anti-HEV IgG, HEV RNA and HEV Ag positive pregnant women transmitted the virus to 12 (85.7 %) of their fetus and the fetus died and terminated to abortion. However, the 7 anti-HEV IgG positive aborted women did not transmit the infection to their fetus. Also, the cor...
M.A.S. El-Kady1, Om-Hashem M. El-Banna 2, E.A Salama2, Salwa N. Zein1
...G and IgG conjugate with alkaline phosphatase was 1:1000. The prepared antiserum was used detection of BSMV by serological methods i.e., Enzyme-linked immunosorbent assay (ELISA), Dot-blot and tissues-blot immunobinding assays (DBIA and TBIA). The presence of the virus was confirmed in mature seed parts and non-seed parts of the different five barley cultivars tested....
Salwa N. Zeinl and Hanaa S. Zawam2
...G and IgG conjugate with alkaline phosphatase was l: 1000. The prepared antiserum was used for detection Of ACLSV using ELISA dot immunobinding assay (DIBA). The presence of the virus was checked in blossom parts. (Petioles, stigma, and flower cup)....
Saiwa N. Zein
... and lgG conjugated with alkaline phosphatase was I : 1000. The prepared antiserum was used for detection of T RV using dot-blot immunobinding assay (DBIA). This is the first report for isolation of Tobacco rattle virus Tohrarirus (T R V) from D.Kaki under the Egyptian conditions.
...
Rashid Saraz1, Saiqa Amur1, Zia-ul-Hassan1*, Naheed Akhter Talpur1, Inayatullah Rajpar1, Muhammad Sohail Memon2, Muhammad Nawaz Kandhro3, Khalid Hussain Talpur1 and Nizamuddin Depar4
...slightly (46%) to medium alkaline (38%) pH, high (44%) to medium (39%) organic matter content, while soils with low organic matter content was comparatively lower (only 17%). Nonetheless, spatial variability map predicted medium organic matter content in the district. Moreover, majority of soils had low (75%) to medium (16%) ABDTPA-P while only small portion (9%) of soils had adequate ABDTPA-P. Contrarily, soils having adequate ABDTPA-K were higher (62%) follo...
Yuyu Wang1,2, Mingzhu Li 3,4, Gang Lin4, Xiaohua Guo5, Aihua Sun5, Hao Dong5, Qinghui Ai1,* and Kangsen Mai1,*
..., specific activities of alkaline phosphatase (AKP) in intestine and purified brush border membrane (BBM) of intestine were significantly higher in fish fed diet S5 than that of fish fed diet S15 (P<0.05). Specific activities of leucine-aminopeptidase (LAP) in intestine and purified BBM of intestine was significantly higher in fish fed diet S10 than that of fish fed diet S15 (P<0.05). Fish fed diets S5 and S10 had significantly higher docosahexaenoic (DH...
Mehran Ali* and Inamullah
...m alternative sources in alkaline calcareous soils have been little investigated. To explore the influence of AM fungi and P management on wheat productivity, two years of field experiments were carried out at Agronomy Research Farm, The University of Agriculture Peshawar during Rabi season 2018-19 and 2019-20. Randomized complete block design was used to test the efficacy of different P sources {1. Single super phosphate (SSP), 2. Rock phosphate (RP), 3. Poul...
Nabi Ullah, Abdul Samad Mumtaz*, Lubna Anjum Minhas, Muhammad Kaleem, Rooma Waqar, Amber Jabeen and Ayesha Hanif
Tehreem Iqbal1, Roheela Yasmeen1* and Faheem Hafeez2
... Liver (Total bilirubin, alkaline phosphate test, Lactate dehydrogenase, and alanine amino transferase) and kidney (Blood Urea Nitrogen, Creatinine, and Uric acid) biomarkers were assessed. The highest levels of ALT and ALP were linked to liver injury and a higher weight in the liver were observed in BPA treated rats as compared to the control group. Similarly, an increase in blood parameters were recorded in BPA treated rats followed by post -treated rats and...
Jaffar Hussain*, Zeenat M. Ali and Syed Farman Ali Shah
...tic. The homogenous were alkaline and acidic. The alkaline catalyst undergoes saponification reaction when free fatty acid concentration was high. The acidic catalyst preparation was expensive and complicate exhibited slow rate of reaction. The biodiesel used in blend form and diesel engines required the modification. The biodiesel sustainable and release no greenhouse gas.
... 
Hazrat Usman1, Shakeel Ahmad2*, Salah Uddin3, Humaira Wasila3 and Yasser Durrani4
...3, indicating acidity or alkalinity levels. Turbidity, a measure of water clarity, varied from 0.5100 NTU to 45.633 NTU. Electric conductivity fell within the range of 261.00 µS/cm to 614.67 µS/cm, reflecting the water’s ability to conduct electricity. Total hardness exhibited values ranging from 180.67 mg/L to 636.67 mg/L, indicating mineral content. These comprehensive data sets contribute to a deeper understanding of the physicochemical pr...
Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.
...nd conjugate of IgG with alkaline phosphatase were carried out to be used for ELISA detection. The concentrations of IgG and IgG conjugated with alkaline phosphatase were 1.0 mg/ml and 1:1000 respectively. Antiserum produced specific to BBTMV was used for virus detection by using different serological diagnostic methods. The percentages of virus detection in leaves, stems were higher than that in roots; on the other hand the...
Maria Kikelomo Adegun1*, Femi Godwin Ekundayo1, David Daisi Ajayi2
...te aminotransferase, and alkaline phosphatase. In all these except alkaline phosphatase, the animals fed 30% GSF and 70% UTCP (T4) fared better than the animals on other treatments. Therefore, when the grasses in tropical regions dry up during the prolonged dry season, sheep can survive on urea-treated cassava peels and Gliricidia sepium fodder. ...
Irfan Ullah1, Asad Ullah2*, Tahira Tayyeb1, Rafiq Ullah1, Muhammad Hanif1, Faiza Khan3, Imad Khan2, Raheela Taj4, Fatima Syed4, Shumaila Gul5, Muhammad Sadeeq6, Muneeb Islam7, Arsalan Khan8 and Khudija Ghani9
Moazama Batool1*, Saima Naz2*, Ghulam Abbas3, Ahmad Manan Mustafa Chatha4, Mamoona Mahmood1, Asma Aziz1 and Fatima Yasmin2
... aminotransferase (AST), alkaline phosphatase (ALP), glucose and cholesterol were significantly (p < 0.05) increased in the treated groups compared to the control group. In conclusion, the study indicates that exposure to cadmium chloride, even in a low concentration, can cause adverse hematological and biochemical changes in Labeo rohita....
Muhammad Azhar1,2, Rida Ahmad3*, Bushra Nisar Khan3 and Zahra Hussain3
...tate transaminase (AST), alkaline phosphatase (ALP), alanine transaminase (ALT), bilirubin, creatinine, blood urea nitrogen (BUN) were determined. Further, different biological, analytical and operational factors which impose significant effects on hematochemical factors were also considered to rationalize the results. Most of the values were in accordance with the mean values published in the literature, but values of few parameters e.g., hematocrit, platelet...
