Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
...changes with an inverted microscope, apoptosis using flow cytometry and statistical significance with one way analysis of variance followed by Tukey’s multiple comparison test. IC50 values of licofelone were determined as 171 µM for 24 h and 140 µM for 48 h applications. Used as a positive control, 250 µM 5-Fluorouracil decreased the cell survival by only 40 % after 48 h, but licofelone treatment with the same dose and time d...
Jam Nazeer Ahmad1,2,*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2, Sumaira Maqsood3, Ishita Ahuja4 and Atle M. Bones4
...e detected using a light microscope. The toxicity of native isolates against S. litura also studied by testing different concentrations (1 x 102 POB (Polyhedral occlusion bodies) mL-1- 1 x 1010 POB mL-1) from the occlusion bodies produced from NPV isolates against 2nd, 3rd, 4th and 5th instar larvae of S. litura. The rapid and sensitive polymerase chain reacti...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
...ity was determined under microscope at10x magnification, morphology and live dead ratio was assessed using eosin nigrosin stain under 40x magnification, concentration was calculated by hemocytometer, membrane integrity was examined by HOST solution. Conception rate was determined by inseminating 20 buffaloes (10 control and 10 best treatment group). After initial examination semen sample having motility ≥ 70% and morphology live dead ratio ≥ 80 % were di...
Babar Tasim1, Tariq Masood1*, Zafar Ali Shah1, Muhammad Arif2, Ata Ullah1, Ghazal Miraj3 and Muhammad Samiullah4
Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*
...served using an inverted microscope, and MDSCs proliferation pattern were determined through growth curve analyses. MDSCs were identified by immunofluorescence and RT-PCR, and the immunofluorescence antibody invoved Sca-1, CD34, CD144, Desmin and CD45 which are the makers of MDSCs. Finally, MDSCs were induced into Adipocytes, osteoblasts, chondrocytes and neuron-like cells by the optimized inducing medium. After the two-step digestion and differential attachme...
Hanif Ullah1, Muhammad Inayat Ullah Khan2, Suleman3, Sawar Khan1,Salma Javed4, Abdul Qadeer1, Mohsin Nawaz1, Sardar Azhar Mehmood4
...pared and examined under microscope. The data was statistically analysed in Microsoft excel. Out of 1750, 214 (12.2%) blood samples were found positive. The higher rate of infection was found under the age group of ten year (14.9%) and lowest rate of infection was found (9.30%) in the age group of 11-30 in female. The maximum number of cases of malaria were detected in August (21.37%) and minimum number (4.8%) in January. The highest prevalence of malaria was ...
Ramazan İlgün1*, Nilgün Kuru2, Ferhan Bölükbaş3 and Fatih Mehmet Gür4
...e examined using a light microscope the tissues and papillae of the tongue were examined with a the scanning electron microscope, and photos of the general histologic structures were taken. The tongue was triangular shaped, and consisted of apex, corpus, and radix sections. The dorsal and ventral surfaces of the tongue were covered by a keratinised stratified squamous epithelium. Lamina propria and submucosal layers were dis...
Muhammad Amjad1,*, Saeed Badshah1, Muhammad Adil Khattak2, Rafi Ullah Khan1, Muhammad Mujahid1
CHARACTERIZATION OF NICKLE FREE TITANIUM ALLOY TI-27NB FOR BIOMEDICAL APPLICATIONS
...ing
electron microscope tests were conducted on TI-27Nb specimens. The tensile strength and young modulus of TI-27Nb
were lower than commonly used Titanium alloy i.e Ti-6Al-4V. The SEM results show mixed brittle-ductile surfaces.
It is concluded that TI-27Nb has lower stress shielding/bone restoration because of low modulus than commonly
used implant materials.
...
Farid Ullah Khan*
ACTUATION ENHANCEMENT OF A MICRO BIMORPH THERMAL ACTUATOR
...ro-mirrors, atomic force microscope probe tips, membranes of micro pumps and micro valves, etc. This research is focused on enhancing BTA actuation characteristics. The mathematical model for BTA is used to study the behaviour of a spectrum of material combinations. Although a silicon-aluminum (SiO2-Al) BTA exhibits superior characteristics over a limited operational range, it is found that a silicon-stainless steel (Si-SS) bimorph provides larger deflections ...
Aamir Khan1, Rabia Noushin2, Mohammad Attaullah3, Shahid Naiz Khan1, Rafiqe Hussain4, Farman Ullah Dawar1, Faiz Ur Rehman1, Muhammad Ijaz1 and Kalim Ullah1*
...es were examined through microscope. Overall incidence of babesiosis was 54.80%.B. bovesshowed high prevalence (35.46%) than B. bigemina (19.33%). Females were more prone as compared to male cattle for both B. bovesandB. bigemina. Cattle having age less than two years showed high positivity for bothB. boves (49.23%)and B. bigemina (26.15%). Similarly rate of positivity for both B. boves (47.12%)andB. b...
Majid Shahi Bajestani1, Esmat Mahdikhani Moghadam2*, Reza Aghnoum3 and Hamid Rohani2
Study of Plant Parasitic Nematodes and Description of New Record (Rotylenchus alius) Associated with Barley (Hordium vulgare L.) in Khorasan Razavi Province, Northeast Iran
...ition, scanning electron microscope (SEM) assay on infected roots by Meloidogyne arenaria showed that this species has been able to create gall in barley root and disrupt on cellular metabolism....
Muhammad Shahbaz1,*, Nosheen Farooq1, Abu ul Hassan Faiz1, Arshad Javid2, Irfan Baboo3, Misbah Shoukat1 and Muhammad Aslam Khan4
...fied under the binocular microscope by following the taxonomic keys of Christophers (1933), Barraud (1934) and Rueda (2004). A total of 2895 specimens of mosquitoes were collected from the study area, belonging to family Culicidae and two subfamilies, Anophilinae and Culicinae. Eleven species were identified as Anopheles barianensis (sub-familyAnophilinae), Culex barraudi, Cx. epidesmus, Cx. fuscocephala, Cx. pipirms fatigans, Cx. pipiens pipi...
Shahid Hussain Abro1*, Mohammed N. Alghamdi2, Muhammad Sohail Hanif3, Hazim Moria2 andHamza Suharwardi1
Caner Öztürk1*, Mücahid Onay1 and Neşe Hayat Aksoy2
...r 30 s. A phase-contrast microscope (400x) was used to determine the sperm motility. PNA-FITC staining was used to examine the acrosome integrity under a fluorescence microscope. Total oxidant status (TOS) and total antioxidant status (TAS) were measured using ELISA. The methionine group showed an increase in the post-thaw motility (53.08 ±3.6%) and viability (47.5 ±2.9) percentages compared to the co...
Salma Javed and Samreen Khan*
...pecies level under light microscope. Afterward, hundred fresh handpicked nematodes were inoculated on both fully grown fungal species at 25ºC. The nematode attraction and mass culture were appeared on Fusarium oxysporum only. On the first week, propagation rate was noted approximately 2.5x10³ followed by 3.9x10³ on the second week and finely after three weeks incubation period the greatest multiplication rate estimated up to 8.9x10³; howeve...
Md. Alal Hossen1, Mohammad Amzad Hossain1*, A.K.M. Munzurul Hasan1,2, Bipresh Das1, Sohel Mian1, Mohammed Mahbub Iqbal1
...re observed under a photomicroscope. Average fertilization and hatching rates varied from 74.33% to 83.89% and from 72.33% to 85.11% respectively among different treatments and control females did not ovulate. The eggs of M. gulio was firmly adhesive and immediately after spawning the average diameter of fertilized eggs were 0.48±0.01 mm. After fertilization, the first cleavage stage of M. gulio occurred within 40 min. The distinctiveness of blastomeres...
Rahat Rukhsana1, Shahnaz Rashid2, Asma Fatima2, Owais Iqbal Khan3, Syed Babar Hussain Shah4, Mumtaz Ali4 and Ghulam Abbas2*
...nkton were counted under microscope. Result showed that water quality parameters of all fish farms were suitable for farming purpose excluding Shamimul Hassan Farm, Fish Farm Green Co. and Ghulam Ali Nizamani Farm, where high level of total hardness was recorded as 1000±2.65 mg l-1, 700±4.80 mg l-1, and 975±4.73 mg l-1, respectively. The minimum chlorophyll-a (1.4-2.1 mg l-1) was found in the ponds water of Shamimul Hassan Farm, Fish Farm ...
Om-Hashem M. El-Banna1, Maisa A.E. Awad2, M.S. Abbas3, Hoda M. A. Waziri2 and Huda S. A. Darwish2
Maha A. El-Abhar1, Moustafa A. Elkady1, Khaled M. Ghanem2, Hussieny A. Bosila3
... using light
microscope showed amorphous cytoplasmic inclusion bodies seemed to be attached to the nucleus
from one or two sites, while those inclusions have never been observed in the epidermal stripes of
healthy leaves. Electron microscopy, revealed cytological and histological changes induced by Alfalfa
mosaic virus infection in potato leaves
Conclusion: In this work, Alfalfa mosaic virus (AMV) cha...
Syed Maaz Nadeem1,*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh2, Arfan Ahmad2, Syed Nadeem Mansoor3 and Muhammad Anees4
...(RGS) and examined under microscope. Results showed a significant difference between the cellularity of normal and CAV-infected birds. While comparing healthy and infected chicken bone marrow, we observed a decreased mean erythroid cell percentage (39.95 versus 17.95%), myeloid cell percentage (57.29 versus 21.98%) and the mean M/E ratio (1.43 versus 1.22), respectively. Bone marrow smears were normocellular among healthy birds while, in diseased chicken, seve...
Allam A. Megahed, Badawi A. Othman, Samar S. El Masry, Abeer A. Faiesal and Mohamed A. Nasr Eldin
...by transmission electron microscope (TEM). Finally, the PVM
isolate was detected using reverse transcription-polymerase chain reaction (RT-PCR) in the sprouts
after dormancy breaking for the harvested potato tubers.
Results: The obtained results demonstrated that, PVM isolate causes severe symptoms on potato plants
under the greenhouse conditions. The infected potato cv. Diamant gave positive DAS-ELISA result
Ahmed K. El-Attar1, Samah A. Mokbel1 and Om-Hashem M. EL-Banna2
...he transmission electron microscope for rapid diagnosis, host reactions, serological
double-antibody sandwich (DAS)-ELISA, reverse transcription (RT)-PCR and nucleotide
sequence determination. Ultra-structural responses of basil leaf cells infected with a
morphologically distinct RNA virus, AMV, were studied. Molecular characterization and
phylogenetic analysis were performed for the AMV coat protein (CP) gene. A...
Gulnaz Ismaylova
...nder a JCM-6000 electron microscope and given dimensions. It turned out that the surface of the egg has a peculiar microstructure (consists mainly of hexagonal and sometimes pentagonal shapes with pyramidal projections at the apex of the corners). The distribution map (scale of 1: 500000) of O. japonica in the study areas was prepared in a digital database using geographic spatial analysis using the ArcGIS (Geographic Information System) program. ...
Nabila Rasheed1*, Bushra Waseem1, Kanwal Haneef2, Shumaila Usman3, Madeeha Sadiq1, Tabinda Urooj1, Sarah Tarique2 and Dabeeran Zeha4
... was done under inverted microscope. Results showed that with each passaging stage the viability of GDM cells remained normal whereas Wound healing potential of GDM-WJMSCs was decreased as compared to controls. Moreover, there were few morphological changes observed between the two groups. In conclusion GDM affects wound healing potential of WJMSCs and morphology of the cells to some extent whereas the viability of cells remains unaffected by GDM.
... 
Noura El-Shahat Attia1*, Abd El-Khalek Ramadan El-Sheikh1, Mohamed Omia Siam2
...ere examined under light microscope for the detection of fungal spores or mites. Blood samples were taken for hematological analysis, along with taking serum samples for biochemical analysis. Rectal temperature, heart, and respiration rates were not significantly differed between the two groups. Packed cell volume (PCV), total erythrothetic count (TEC), white blood cells (WBCs), serum glucose, total proteins and serum Zn were significantly decreased. Moreover,...
Min Li1, Shiwu Deng2*, Yiqian Peng3 and Hong Li2
...by transmission electron microscope. The activity of Caspase-3 in heart tissue of SD rats was measured, and the expression of p-JNK, GRP78, Caspase 12 and CHOP in heart tissue of SD rats was determined by Western blot. We found that LVEDd and LVESd in MIRI group and MIRI + DEX group were significantly higher than those in sham operation group (P<0.05), and LVEF was significantly lower than that in sham operation group (P<0.05). Compared with MIRI group, ...
Jiang Wu*
...mental scanning electron microscope (ESEM) was used to analyze morphological changes of E. coli and Staph. aureus cells. ATP analysis kit and EnSpire microplate reader were used to detect the intracellular ATP concentration of 4 foodborne pathogens. According to our results of all the tested samples, 38 (28.57%) were found to have Staph. aureus, 27 (20.30%) had Salmonella, 46 (34.59%) had E. coli, and 22 (16.54%) had L. monocytogenes. Staph. aureus had MIC of ...
Zahra Jamilah Sabrina1, Adrian Pearl Gunawan2, Beryl Reinaldo Chandra2, Ilham Pangestu Harwoko3, Johannes Marulitua Nainggolan4,Widi Nugroho1*
Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1*
...nd detected under stereo-microscope for fried egg colonies. Forty-three samples were detected as Mycoplasma spp., using PCR for species identification, MG, MS, and Untyped strains, with a high prevalence of MG (27.9%) and lowest for MS (8.8%). An rRT-PCR used for detection of AIV-H9N2 and IBV showed high prevalence for IBV (88.2%) and AIV-H9N2 (52.9%), the highest rates of co-infection were MG-H9N2-IBV, Mycoplasma other than MG/MS, and H9-IBV with rates of (...
Aiman Batool1, Muhammad Sohail Sajid1,2*, Hafiz Muhammad Rizwan3**, Asif Iqbal4, Imaad Rashid5, Ibadullah Jan6, Faiza Bano7, Fiaz Ahmad8, Waqas Ahmad1, Muhammad Nisar Khan1
...ried out under the stereomicroscope. The overall prevalence of GI parasites, haemoparasites and ectoparasites in the small ruminant population was 74%, 16% and 53%, respectively. Sheep were found more prone to endoparasites conversely ectoparasites were more prevalent in the goat population. A higher prevalence of GI, haemo and ectoparasites was observed in young animals, females and Damani breed of sheep and goat. Among the extrinsic factors, a significantly ...
Gamalat Y. Osman1, Elham M. Hassan1*, Tarek A. Salem2, Azza H. Mohamed1
... burden. A scan electron microscope examination of male worms showed tegument corrosions with reduced spines density and abnormal spine tubular shape. Treatments of infected mice with FEW or PZQ significantly reduced hepatic and intestinal total egg count, while showed a significant increase in dead egg percentages and reduced mature egg count in oogram pattern as compared to infected control mice. Hepatic granuloma diameters in FWE treated infected mice were ...
Basel A. Abokhadra, Samah M. Mosad, Sahar Abd El Rahman*
...ce under the fluorescent microscope. The phylogenetic tree constructed from partial sequencing of gB of our isolate was divided into two clades; clade 1 BoHV-1 (BoHV-1.1 and BoHV-1.2) clade 2 (BoHV-5). Our isolate showed 100% homology to BoHV-1.1 reference strains away from BoHV-5 reference strains that were clustered in the other clade. BoHV-5 reference strains were different from our isolate by 25 nucleotide substitutions, which led to five amino acid substi...
Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*
...served under an inverted microscope. Based on the growth curve test, we found that the growth mode of the cells was “S” type growth. The clonogenic assay was performed to determine the clonogenic efficiency CPSCs. The expression of cell surface markers was detected by immunofluorescence, flow cytometry, and RT-PCR, and it was found that the CPSCs positively expressed FGFR-3, collagen type II, CD44, CD73, CD90, vimentin, Notch1, CD105, and CD106, bu...
Bnar Shahab Hamad1, Bushra Hussain Shnawa1*, Rafal Abdulrazaq Alrawi2
...e examined under a light microscope. The histological alterations caused by the hydatid cyst were cell degeneration, necrosis, hemorrhage, congestion, and fibrosis. The findings revealed that the CD3 T cells were the most predominant inflammatory cell in hepatic mice tissue. Also, CD4 Helper T cells, CD8 Killer T cells, CD20 memory cells, and CD68 macrophages were detected in the infected mice. Most samples of mice expressed a different level of tissue necrosi...
Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2
... were viewed under light microscope. Extracted infectious sap of N. glutinosa leaves was examined using Transmission Electron Microscope. Total RNA was isolated from fig leaves showing symptoms and symptomless. Phylogenetic relationships were evaluated. Virus particles were detected by DAS-ELISA in extracts from inoculated host leaves. The virus was transmitted by grafting on fig plants and sap inoculation on a very restrict...
Ayesha Saeed1*, Farzana Kishwar2, Ahsan Nazir3, Sharjeel Abid4, Muhammad Abiodullah5, and Danial Ali6
A. Sattar1, †, A. Khan2, N. Khatoon1 and A. Mujahid3
...and observed under light microscope with the help of regular parasitological
methods. The nematode parasites namely; Raphidascaris acus (Bloch, 1779) larvae, Metabronema magnum (Taylor,
1925), Haplonema immutatum (Ward et Magath, 1917) and Hedruris bryttosi (Yamaguti, 1935) were recorded from
the gut of the catfish, Arius arius (Hamilton, 1822). These are new host records as these parasites have not been
reported...
Belema Robert1, Favour Welenya1, Deborah Achi1, Cynthia Onyeagwara1, Soala Obie Minimah2, Ebele Anulika Obichi2, Chidinma Charity Amuzie1*
...applied on a grease-free microscope slide; a drop or two of Lugol’s iodine was added to it. This was then covered with a cover slip and viewed under the light microscope at x4 and x10 objective lens. Results: Only S. stercoralis was isolated from the samples examined. Water leaves (50%) and pumpkin leaves (11%) were infected. Bitter leaves and scent leaves were uninfected. The parasite was encountered in all locations ...
Dina N. Ali1*, Sayed H. Elhabtey2*, Manal M. Amin1
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...ity with contrast-phase microscope (eosin-nigrosin staining) was investigated. Moreover, superoxide dismutase (SOD) assay, a fatty acid composition with gas chromatography and lipid peroxidation TBARS (Thiobarbituric acid reactive substances) content were used for oxidative stress evaluation. Frozen-thawed results showed significant (p < 0.05) enhancement in all sperm parameters with 10 ng/ml of DHA supplementation. DHA improved (p < 0.05) SOD levels in...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
...matozoa motility using a microscope, the viability with the eosin-negrosin staining method, and membrane integrity using the hypoosmotic swelling test (HOST) at the fresh semen, 5th, 24th, and 48th hours post-cold storage. The results revealed that in the 5th hour, percentage of motility, viability, and membrane integrity were not significantly different. However, at the 24th and 48th-hour results revealed significant differences by adding honey (P < 0,05)....
Magda A. Eldomiaty1,2, Manal E. Elsawaf2, Soad S. Ali3, Heba El-Sayed Mostafa4,5*
...es by light and Electron microscope, and for immunohistochemical expression. Rats from group III showed statistically increased corticosterone level and increased cortical thickness compared to other groups. Voluntary exercise improved these measures in rats of group IV. Histological disorganization of cortical cells was observed in group III with manifest large cytoplasmic vacuoles. Enormous increase in the number of both apoptotic and proliferative cells was...
M. A. Haq, M. Shahid and K. Mahmood
.... Under the stereoscopic microscope mature whitish pear shaped females were isolated from the roots of C. arvensis. By using perineal pattern technique (Eisenback
Muhammad Ramzan1*, Unsar Naeem-Ullah2, Shafia Saba2, Madiha Mobeen Khan3, Umair Faheem4, Asad-ur-Rehman3, Naeem Arshad Maan3 and Waseem Hassan5
...xonomic keys under stereomicroscope. After identification, it was revealed that the insect under study was identified as T. varians. After going through the relevant literature, it was found that this species had not been reported so far from Pakistan. Therefore, the detail description is being presented here for futuristic references....
Muhammad Ahsan Riaz1, Ayesha Riaz2, Muhammad Asif Mahmood3, Muhammad Uzair Mukhtar3*, Zaib Un Nisa1, Beenish Ijaz4 and Muhammad Shahid Rasool5
...arried out using a stereomicroscope, which included changes in head length and width, thoracic length and width, abdominal length and width, and total length of the larva. Every month, the fourth instar larvae (n=36) were investigated for body size measurement. The results showed that low temperatures of breeding water significantly increase (P≤0.05) the body size, head, thorax and abdomen of larvae. The results convinced that temperature inversion affects ...
S.A. El-Kewey1; R.A. Omar1; S.A. Sidaros2 and Samaa Abd El-Khalik3
... cultivars. The electron microscope preparations of crude sap extracted from naturally infected garlic plants and negatively stained with 2% PTA showed flexuous rod-shaped particles 657-714 nm long. Biological relationships between the virus and insect vectors were studied....
Shabana Mangi1*, Abdul Manan Shaikh1, Waheed Ali Panhwar1, Javeed Ali Ujjan1, Fakhra Somroo1, Shazia Praveen Solangi2, Sumbul Mureed Mastoi3 and Ranjeet Kumar1
...th cameras fitted on the microscope. This revealed the occurrence of 46 specimens and identified 05 species under the subfamily Asopinae. Z. caeruleas (Linnaeus 1758) predatory stink bug is premalinary described from the district Khairpur Sindh province of Pakistan. Photographs of the adult as well as male and female aedeagus, conflicting on the biodiversity table are provided....
Yun Zhang*
...by transmission electron microscope. Western blotting was done to detect the protein expression. Transmission electron microscope analysis revealed that exosomes could be significantly observed in the PE group. The expression of surface markers that included CD63 and CD81 were dramatically upregulated in PE group. The protein level of ET-1 and Ang-II were significantly increased in PE group, and the upregulated ET-1 and Ang-...
Thekra Fadel Saleh, Omar Younis Altaey*
...cal analysis. Dissecting microscope measurements and histological measures were taken, and immunohistochemical analysis was performed to evaluate B lymphocyte distribution. The study found that in Muscovy ducks, the left caecum was longer and heavier than the right caecum, and the proximal portion of both caeca was wider and had thicker walls compared to the middle and distal portions. Histologically, the length of villi and depth of crypts were greater in the...
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
... confocal laser scanning microscope for sperm membrane integrity parameters. Sperm lipid peroxidation was also analysed through malondialdehyde production. The study found that there was a gradual decrease in all sperm quality indicators of all the treatments during 12 days of storage. In addition, the chilled sperm motility parameters among all the semen extenders were not noticeably different at each time period of evaluation (P>0.05). Although sperm lipi...
Vui Van Nguyen1, Samorn Ponchunchoovong2, Sajeera Kupittayanant3, Pakanit Kupittayanant2*
... analyzer and a confocal microscope to analyze motility and membrane properties, respectively. Additionally, malondialdehyde production was measured to evaluate lipid peroxidation capacity. The results revealed that incorporating essential oils at concentrations under 100 µg/ml in semen extenders had a positive impact on sperm quality. Nonetheless, employing essential oils at elevated concentrations of 200 µg/ml exhibited detrimental impacts on the...
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
Nabi Ullah, Abdul Samad Mumtaz*, Lubna Anjum Minhas, Muhammad Kaleem, Rooma Waqar, Amber Jabeen and Ayesha Hanif
...nomically by using light microscope. In all studied sites genus Scenedesmus was dominant with fifteen species in both seasons, while all other micro-algal species were dominant at site E1, Salarzai Thali Dam in March spring session. During the study period, a total of 36 green micro-algal species and 16 blue-green micro-algal species were documented. Additionally, the sampling sites underwent thorough water physico-chemical analyses to explore any potential co...
Eisa*, Nawal A.; Abd El-Ghafar **, N. Y. Abd EL-Mageed*, M.H.; Mohamed* , F.G. and Hasan*, Eman O.
El Sayed l , Yasmein A.; Ghareeb2 , A.; El-Dougdoug3, K. A. and Bekheetl , H. K.
...nfected extract by light microscope showed polyhedral bodies with concentration (2.3x107PIB/ml). In our study the morphological structures of S.exigua infected with SeNPV (EG) as well as healthy one were examined by SEM. It was showed complete destruction of external larval body, reduction in each abdominal segment size, pro-legs and thoracic legs reduce in size and appear short and stumpy also the oval shape spiracle converted to circular shape with rupture a...
A. A. Kheder1; I. A. M. Ibrahim2; H. M. Mazyadl
...cal properties, electron microscope (EM) and reverse transcription Polymerase Chain Reaction (RT-PCR). Field survey was carried out in commercial peach orchards during 2001-2003 in four different locations using DAS-ELISA. The average percentages of infection in three seasons were 17.5% and 3.1% in El-Dakahlia and El-Behira respectively. Naturally PRMV-infected trees develop chlorotic spots, rosetting, mosaic, chlorotic mottling, leaf deformation and shortenin...
A-New-Whitefly-Transmitted-Geminivirus
...y transmission. Electron microscope studies of TYMV showed geminated virus particles with about 18-20 x 30 diameters nm. The molecular weight of the viral capsids was estimated to be 28 KDa for TYMV. Serological analysis also proved that the putative TYMV belongs to whitefly- transmitted geminivirus by ELISA analysis using the whitefly transmitted-geminivirus (WTG) antiserum [immunogenic oligopeptides coat protein (Cp-3)]. Based on the previous results, the is...
B. A. Othman; Kh. A. El-Dougdoug; M. H. Abdel Ghaffar and T. F. El-Arabi
...in transmission electron microscope they showed particles have isometric heads or about 52.5, 58.8 and 64.2 nm in diameter with long contractile tails of about 100x30, 80.2x29.4, and 102x33.5 nm, respectively. While the RM4 phage had an isometric head of about 37.5 nm in diameter with short tail of about 12.5x 5 nm. SDS-PAGE indicated that the phage RM1 contained 6 structural proteins of 62.4, 60. 53.4, 50, 28, and 20 kDa; RM2 had 5 structural proteins of 60.4...
Ahmed M. Manthoor, Ali H. Saliem*
Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*
...ng Transmission electron microscope (TEM) and further to investigate the mRNA gene expression of MyD88 (Myeloid differentiation primary response 88), CCL5 (C-C Motif Chemokine Ligand 5), MDA5 (melanoma differentiation-associated protein 5) IKBKE (inhibitor of nuclear factor kappa-B kinase subunit epsilon), NFKBIA (NF-kappa-B inhibitor alpha), NFKBIE (NF-kappa-B inhibitor epsilon), Interferon Alpha (IFN-α), cMGF (chicken myelomonocytic growth factor), and...
Xin-yu Han1,2, Afrasyab Khan1,2, Ali Raza Jahejo1,2, Qian-qing Cheng1,2, Meng-li Qiao1,2, Raza Ali Mangi1,2, Muhammad Farhan Qadir1,2, Ding Zhang1,2, Ying Wang1,2, Yu-hai Bi1,3, Rui-wen Fan1,2 and Wen-xia Tian1,2*
... a transmission electron microscope (TEM). The mRNA gene expression of TLR1, 2, 3, 4, 5, 7, 15, MHC I, II, and MyD88 in M. synoviae infected chicken erythrocytes was determined using quantitative real-time PCR (qRT-PCR) at four different time intervals (0, 2, 6 and 10 h) post-infection and compared to uninfected controls. The mRNA expression of TLRs such as TLR1, 2, 3, 15, and MHC II were significantly upregulated at 6 and 10 h post-infection in infected chick...
ADEEBA SYED1, DILAWAR HUSSAIN2, UZMA RAFI1 & SUMAIRA MAZHAR1*
...e examined under digital microscope. Pronounced histological changes like necrosis, infiltration, atrophy, shrinkage and degeneration of intestine was observed for different CPF concentrations. Kidney sections of Labeo rohita under different CPF concentrations exhibited nuclear hypertrophy, vacuolar degeneration of glomeruli, and occlusion of tubular lumen, cloudy swelling degeneration and hyaline droplets degeneration. In the liver tissue prominent histologic...
Yousef Abdal Jalil Fadladdin1, Ateeq Ullah2*, Raheela Nawaz3, Yagoob Garedaghi4, Abbas M.A. Al-Azab5 and Mashael Abdullah Aldamigh6
...thods and examined under microscope first under low power objectives and then higher power Lense. Evidence of infection was noted by the presence of helminth eggs. Out of 200 samples 65% (n=130) were found to be infected with 2 species of soil transmitted helminths including 27% (n=54) A. lumbricoides and 35.5% (n=71) A. duodenale. Regarding ages, 11-12 years were highly infected. Females were observed to be infected more than male students. Children of unempl...
Fawad Khan1, Zahir Muhammad1, Khushdil Khan2 , Shabir Ahmad2, Muhammad Jamil Khan3, Tamana Bakht4 And Asif Kamal2
...hrough light
microscope (LM) and scanning electron microscope (SEM), the samples were prepared via
acetalization process. We done the fieldwork for the collection of weed plants and also
performed the experimental work i.e. SEM and LM for the pollen morphology. Most of the
species recorded with pollen of tricolporate and echinate. Species belonging to Asteraceae
wer...
Tanvir Hussain, G. M. Nasir and Ayaz Khan
...ared, observed under the microscope and data were collected for the frequency and dimensional measurement of different wood elements/ structures in each wood species. On the basis of results, it was found that Morus alba wood may be comparatively stronger and have better seasoning and preservation behavior. Moreover, Morus alba wood may be more resistant to deteriorating agents in comparison to Morus nigra. Further, the wood of both the sp...
Khalid Hussain, G. M. Nasir and Tanvir Hussain
...d and examined under the microscope. Data were collected for ring growth rate, heartwood and sapwood percentage along with different dimensions of fiber, vessel and wood rays of each species. Results showed that maximum average ring growth rate was found in Paulownia catalpifolia and the highest heartwood percentage was observed in Paulownia tomentosa. Maximum fiber length and fiber diameter was measured in P. catalpifolia and the lowest i...
Ghulam Mustafa Nasir, Tanvir Hussain and Khalid Hussain Sulangi
...ared, observed under the microscope and data were collected for the frequency and dimensional measurement of different wood elements/structures in each wood species. On the basis of results it was observed that Kiker, Robinia, Lachi and Gurgrua wood may be strong and hard and Toot, Kangar, Siris, Willow, Barh, Batkar and Jaman wood may be better or medium in strength due to longer, thick-walled or narrow lumened fibers. The wood of all the studied species may ...
Ghulam Mustafa Nasir
...d and observed under the microscope for various structural features. Results revealed that Babul and Phulai wood may be strong and hard and Jand, Mulberry, Ghaz, Ash and Ber wood may be better in strength and moderately hard due to longer, thick-walled or narrow lumened fibers. The Apple wood may be medium in strength and comparatively soft because of thin-walled and wide lumened fibers. The wood of all the studied species may be non-durable due to higher freq...
Ghulam Mustafa Nasir
...d and examined under the microscope for various structural features. Data were collected for the frequency and dimensional measurements of various wood elements / structures in the wood. Results showed that minute variations exist in general in average values of different anatomical features in Masquite wood grown in both areas. In Masquite wood grown in Punjab, the fibers are fairly longer and thick-walled, the wood rays are smaller in size and lower in frequ...
Ghulam Mustafa Nasir
...s and observed under the microscope for structure, frequency and dimensional measurements of different wood elements/structures in the wood species. Results showed that in Acacia ampliceps wood, the fibers are medium in length and reasonably thick-walled. The vessels are small in diameter and lower in frequency. The wood rays are higher in frequency and a bit larger in size. The wood may be better in strength and can be used for various wood products. P...
Ghulam Mustafa Nasir
...d and observed under the microscope for various structural features and data were collected for the frequency and dimensional measurements of different wood elements and wood rays. Results showed that the wood of Neem, Mesquite, Kabuli Keker and Beri Patta may be stronger or better, Alstonia and Jacaranda medium and that of Sohanjna weak in strength on the basis of fiber morphological characteristics. The wood of all the studied species may be somewhat non-dur...
G. M. Nasir and Iqbal Mahmood
...e and observed under the microscope. For physical and mechanical properties (both in green and air dry conditions) the wood samples were prepared and tested according to ISO and ASTM standards. Results showed that Robinia wood is better in strength due to thick walled and long fibers. It is stronger than Shisham wood in different mechanical properties. The wood may be less durable but can be easily preserved with chemicals. Similarly the wood can be seasoned w...
G. M. Nasir and Noreen Fatima
...s and observed under the microscope for various structural features. Data were collected for the frequency and dimensional measurements of different wood elements/ structures in each species. Results showed that on the basis of fiber morphological characteristics, the wood of Phulai, Ipip Ipil, Black Siris and White Siris may be stronger or better in strength whereas, Pipal, Lasura and Jand may be medium and Gul-i-Nishtar light in strength. Due to abundant axi...
Ghulam Mustafa Nasir, Noreen Fatima and Kanwar Muhammad Suleman
...s and observed under the microscope for the structure and dimensional measurements of various wood elements/ structures. Results showed that in Amaltas, Ber, Bakain, White Bakain and Paper mulberry woods, the vessels were sufficient large in diameter and in Chinar and Paper mulberry woods, the frequency of vessels was found to be higher. Therefore, the woods may be easily seasoned and preserved. Amaltas, Chinar, Paper mulberry, Ber, Bakain, White Bakain, and F...
Khalid Hussain and G. M. Nasir
...s and observed under the microscope. Data were collected for the frequency and dimensional measurements of different wood elements/structures. Results revealed that in Elm wood, the fibers were longer, medium in diameter, wide lumened and fairly thick-walled due to that the wood may be relatively better in strength. The vessels were lower in frequency but quite larger in diameter and the wood can be seasoned without any difficulty. The wood rays were medium pe...
G. A. Bajwa and Hanif Gul
...asured under a binocular microscope (50X). Five larval instars were determined with maximum and minimum ratio of increase in head capsule width of 0.748 and 0.624 with an average 0.70 which conforms with Dyar's rule. The pest was found active in all larval instars from April to October except June and it over wintered in larval stage (2-5th instar) from November to February. Six to seven generations were found in a year....
Nada Hamzah Shareef Al Shabbani1, Marwa Jamal Hussain Al Kinani2*, Tmara Qais Al-Mohammadi1
...s of tissues under light microscope with different magnification power. We have observed excessive males and the prevalence of SCABies were more in a young adult group in comparison with other age groups. It was found that lesions with wiry projections tended to persist for two weeks to 52 weeks and the number of nodules had varied from one to fourteen lesions. Examination of Histopathological slide showing acanthosis in 80, histolymphocytic infiltration in 76...
Lin Li1*, Lu Lu2, Gang An2 and Xiaoguang Zhang3
...erved under the inverted microscope electron microscope. The apoptosis of the cells was assayed by Hoecht staining. The expressions of TF, Bax and Bcl-2 in the cells were determined by Western blot. After treatment with TF-TP 150 μmol/L, the cell monolayer adhered to the wall, which was fusiform or polygonal. The suspended cells and cell debris were significantly reduced compared with the blue light model group. Hoecht st...
Md. Asaduzzaman Lovelu1, Md. Amir Hossain2*, Md. Uzzal Hossain1, Tanzila Zafrin Tanvi1, Mahfuza Ferdous3, Nazmin Sultana Runa4, Assrafi Siddika3, Md. Sahidul Islam2
...e examined under a stereomicroscope and stained with Giemsa’s stain. Among the investigated cattle, 37.88% had tick infestations: 14% had Rhipicephalus (Boophilus) microplus, 28.01% had Haemaphysalis bispinosa, and 4.13% had mixed infections. Adult cattle older than 2.5 years old (41.05%) had the highest prevalence of tick infestations, while calves younger than a year (33.57%) had the lowest prevalence. Crossbred cattle were more susceptible to infectio...
Zhulmaydin C. Fachrussyah1*, Indra G. Ahmad2, Iin S. Lantu3, Arafik Lamadi2, Wila R. Nento3
Shaymaa Jabbar Hassoon1*, Hiba Ibrahim Ali2, Ayat Jasim Mohammed3, Oras Salman4
...nd transmission electron microscope (TEM). The results showed the meat samples comprised Staphylococcus aureus (14%), Escherichia coli(8%) and pseudomonas(2%) , all isolated bacteria were confirmed by VITEK 2 system. The results of UV-Vis analysis showed a silver surface plasmon resonance band at 420 nm. AgNPs’ spherical form and diverse diameters D1 (21.10) and D2 (21.39) were observed using SEM. While FT-IR spectrum noticed the existence of diverse eff...
H.A. Abdul-Ratha1*, Sahar Mahdi Hayyawi2, Essam F. AL-Jumaily3
... under scanning electron microscope. Forty eight Staphylococcus aureus isolates were diagnosed from the total 142 cows milk samples collected from different areas.These isolates were produced biofilm with different thickness. Six Staphylococcus aureus isolates out of forty-eight shown higher thickness (3mm) of biofilm with protein concentration 62, 66,70,72,80 and 85 mg /ml. Twenty-five Lactobacillus acidophilus isolates were diagnosed from samples of healthy ...
