Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*
...tem samples (lymph node, spleen and trachea) of goats and a sheep during the year 2008-2012. The partial N gene of ten isolates and partial F gene of eight isolates were amplified by RT-PCR, and were sequenced. The phylogenetic analysis revealed that PPRVs circulating in Bangladesh belonged to Lineage IV and they formed a separate sub-cluster along with recent isolates from Nepal, Bhutan and China. On genetic analyses, it appeared that the N gene of PPRV is le...
Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*
...ny body organs including spleen. However, the effect of Plasmodium chabaudi infection on the spleen of the murine lupus model BWF1with the comparison between live and gamma irradiated parasite was not studied before. A total of 30 female BWF1 mice were randomly divided into three groups as follows: group (I) control group (lupus uninfected); group (II) live P. chabaudi infected group (lupus + live P. chabaudi infection); and...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...sions observed in bursa, spleen and thymus and compared with control. Mild to severe hemorrhages and lymphocytic depletion, follicle necrosis, leukocytic infiltration, cyst formation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
... including brain, heart, spleen, kidney, intestine, gill, liver, skin, blood and muscle of common carp. Expression profiles provided us more evidences to understand GST gene functions as well as their functional evolution post duplication. Overall, the whole set of GST genes provide essential genomic resources for future biochemical, toxicological and physiological studies in common carp.
...
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and Kui Chen2,*
... were expressed in gill, spleen, heart, lips, skin and tongue tissue, suggesting that it is not only act as taste receptors, but also play other possible functions. Furthermore, the cloning and expression analysis of T2R1 provide theoretical basis to further study function of T2R1 for T. fasciatus.
...
Tiansen Li1, Meiling Huang2, Zhen Wang1,Fei Guo3,Hui Zhang1,* and Chuangfu Chen1,*
...08. With decrease in the spleen/body weight ratios of mice infected with omp10 mutants were noted, inhibition of phagosome-Lysosome fusion was dramaticly impaired, and profound increase of apoptosis rate after exposure of RAW264.7 to omp10 mutants at 12 h, 24 h and 48 h compared to RAW264.7 exposed to 2308. Results indicate that Δomp10 mutant would affect its survival and pathogenicity.
...
Rabia Tahir1*, Khushi Muhammad1, Masood Rabbani1, Muti-u-Rehman Khan2, Farah Khan1, Hassan Bin Aslam1 and Arslan Mehboob3
... this purpose, liver and spleen samples (n=90) were collected from day-old-chicks derived from breeders at 14, 21 and 30 days of post-infection with AAV-4. The presence of AAV-4 DNA was detected through PCR. Chicks from virus-challenged breeders failed to show a clear and expected PCR positivity. The connotations derived from these findings are that vertical transmission via transovarian route is reported the field conditions. However, based on genetic detecti...
Farid S. Ataya,1,2,* Dalia Fouad,3,4 Ajamaluddin Malik,1 Nikolaos E. Labrou,5 Mohamed S. Daoud1,6 and Hesham M. Saeed7
...stis, followed by liver, spleen, kidney and lung. CdGSTP1-1 was heterologously expressed in Eschericia coli BL21(DE3) as a ~24 kDa soluble protein and showed to be catalyticly active towards the model substrate 1-chloro-2,4-dinitrobenzene. The results of the present study provide new information into camelid evolution and give further insights into the diversity and complex enzymatic functions of GST superfamily.
...
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
...er, heart, lung, kidney, spleen and small intestine. The results showed that: The expression level of BPI in lung of Rongchang pig was relatively higher level compared to that in heart, liver, kidney, spleen and small intestine. The expression level of BPI in liver of Landrace was relatively higher level compared to that in heart, lung, kidney, spleen and small intestine. Real-time fluores...
H.I. Hosein1, Sherin Reda Rouby1*, Ahmed Menshawy1 and Ahmed E. AbdAl-Ghany2
...y lymph nodes, udder and spleen as well as milk of 46 lactating cows were subjected for bacteriological studies for isolation and identification of Brucella organisms. Brucella melitensis biovar 3 could be recovered from 64 (61.5%) tissue specimens and 28 (60.9%) milk samples. Brucella cultures were further identified on molecular basis using universal and Bruce ladder PCR. ...
Soraya Khosravian Dehordi*, Abdolnaser Mohebbi and Kahin Shahanipour
...ative status in the rats spleen exposed to the cadmium (Cd) metal. For this purpose, thirty male rats equally divided into six groups, group I received nano-Se (0.1mg/kg) alone as dose, group II selenite odium (0.1mg Se/kg) alone, group III Cd chloride (300mg/250cc), group IV Cd along with nano-Se, group V Cd along with selenite sodium and group VI (control) given (1 ml) saline. All doses was orally (gavage) given to rates.Protein carbonyls (PC) andthiobarbitu...
Saba Parveen Samo1, Moolchand Malhi1,*, Javed Gadahi2, Yan Lei3, Allah Bux Kaciwal1 and Saeed Ahmed Soomro1
...liver, heart, kidney and spleen were not significantly different (P > 0.05) between the groups, however, the lung weight increased in B (1.15 ± 0.04 vs 0.99 ± 0.04, P < 0.05) compared to A. Digestibility trails revealed that utilization of dry matter (DM), crude protein (CP) and crude fiber (CF) increased (P < 0.05) by 13.71, 12.02 and 4.78% at week 3 and by 11.11, 11.8 and 3.46 % at week 6 in B compared to A. Among carcass characteristi...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
...on levels in duck liver, spleen and bursa of Fabricius were examined after a model for immune stress was implemented through LPS injection in Peking ducks. The results from this assay suggested that LPS injection significantly increased the concentration of tumor necrosis factor-α (TNF-α) in bursa of Fabricius (P<0.05), along with an increase in the concentrations of immunoglobulin-G (IgG) and interleukin-12 (IL-12) in the serum of ducks ...
Hazrat Nabi1, Irshad Hussain2, Muhammad Adil3, Amar Nasir3,*, Arbab Sikandar3, Saeed Khan1 and Nasrullah Khan3
... weight ratio of thymus, spleen and Bursa of fabricius of aflatoxins binders administered groups (groups E and F) were also significant compared with ones fed aflatoxins mixed feed (groups B and C). In conclusion, mycotoxin binders counteracted the deleterious effects of aflatoxins on antibody profile, weight gain, feed consumption and visceral organs of broilers suggesting that these agents can be potential ameliorators against aflatoxicosis in broilers.
Jingen Xu1, Yang Liu2, Erhui Jin1, Youfang Gu1, Qin Zhang3 and Shenghe Li1,*
...ranscriptome profiles in spleens of Yorkshire pigs with different peripheral blood T lymphocyte subsets. The results showed that 2050 differentially expressed transcripts were identified, with 1554 up-regulated and 496 up-regulated in high and low T lymphocyte groups, respectively. Among differentially expressed genes, IL1B, IL17F, IL6R, CXCL2, CCL20 and CCL25 were selected based on their immunological functions and CD8B and CD81 ma...
Naila Chand1, Shamsullah1, Rafiullah1, Rifat Ullah Khan2, Muhammad Mobashar3, Shabana Naz4,*, Ebrahim Rowghani5 and Murad Ali Khan2
...ight of bursa,thymus and spleen. Significantly higher villus height, crypt depth and goblet cell count was recorded with MOS-100 while values lower with MOS-0 treatment. It was concluded that improvement in growth performance, intestinal histomorphology and relative weight of lymphoid organs in broiler chicks during starter phase was attributed to the supplementation of MOS.
...
Muhammad Tufail1, Naila Chand1, Rafiullah1, Shakoor Ahmad2, Rifat Ullah Khan2, Muhammad Mobashar3 and Shabana Naz4,*
...ght of bursa, thymus and spleen. Significantly higher villus height, crypt depth and goblet cell count were recorded in MOS-100. It was concluded from the results that improvement in growth performance, intestinal histomorphology and relative weight of lymphoid organs in broiler chicks during finisher phase was attributed to MOS at the level of 100 mg/kg.
...
Kalimullah Khan1,*, Hafsa Zaneb2, Zia Ur Rehman3, Hamza Maris4 and Habib ur Rehman1
...f heart, liver, kidneys, spleen, gizzard and intestines. Results showed that supplementation of phytase enzymes affected the weight and length of small intestine and feed conversion ratio (FCR) and did not affect the other zoo technical variables like body weight, feed consumption, and weight gain during the whole experimental period. Application of phytasefailed to exert any influence on the weights of gizzard, provetriculus, heart, liver,
Yang Liu1, Jing-xin Mao2, Xiao-dong Wei2, Man Yi2, Xiao-long Zhang2, Ke Zheng3, Xian-xin Chen4, Guo-Ze Wang5 and Bing-bo Chen1,*
...fference was seen in the spleen index between the mixed fermentation BFA group and the control group (P<0.01). Immu-serological indicators: the mixed fermentation group had significant increased serum levels of IgA, IgG, IgM and IL-2 compared with the control group (P<0.05); the Lactobacillus BFA group had obviously increased levels of IgA, IgG and IL-2 than the control group (P<0.05). It proves that the BFA has a positive effect on improving the heal...
Attaur Rahman1, Sadeeq ur Rahman1,*, Ashfaq Ahmad2, Abdul Sajid1, Muhammad Asif Hussain1, Mian Muhammad Salman1, Niamat Ullah Khan1, Kamran Khan3, Fazli Rabbani1, Muhammad Akbar Shahid4, Irshad Ahmad5 and Shakoor Ahmad6
...hepatitis, nephritis and spleenomegaly. Altogether, albeit, FAdV-4 caused similar symptoms and lesions in ostriche, however, mortality remained comparatively lower....
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
...peripheral blood and the spleen. Western blotting was performed to detect Stat5, Bcl-2, and Cyclin D1 protein expression in peripheral blood.The red blood cell count and hemoglobin levels decreased in 5-Fu group compared with the control group (P < 0.05). 5-Fu+APs group showed a marked increase in the number of erythrocytes and a significant increase in hemoglobin content (P < 0.05). Compared with the control group, 5-Fu group showed a sign...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1, Muhammad Faheem Malik3 and Muhammad Idrees4
...e bile fluids, liver and spleen tissues were collected from overnight dead layer chickens (n = 8) from Pattoki region of Punjab province, Pakistan, during July to August 2016. The RT-PCR showed the amplifications of selected regions of helicase (186 bps, 2769-2954 nt) and capsid (280 bps, 5461-5741 nt) domain in the bile fluids of two birds. The histological data demonstrated pathological changes in liver and spleen tissues ...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...osis in the liver, lung, spleen, kidney, testicle, and lymph nodes, among which changes in the spleen were the most serious. Immunohistochemistry results demonstrated the detection of B. canis antigens in the lesions of all examined tissues. Strong positive staining was primarily found in the spleen, liver, and testicle. In conclusion, this study was the first to report the isolatio...
Gong Ga1,2 and SuoLang Sizhu2,*
...issue ((including liver, spleen, kidney and intestine, n=21) by nested RT-PCR and qRT-PCR. HEV antigens were detected in tissues by immunohistochemistry analysis. Overall, we found that that eight serum samples (4.6%, 8/173) were positive to anti-HEV IgM antibody and Seventeen serum sample (9.8%, 17/173) were positive for anti-HEV IgG antibody. The HEV RNA positive rate was of 4.6% (8/173) in feces and serum. Moreover, the prevalence of HEV RNA was 9.5% (...
Mohsin Ali Alias Mehran Khan Solangi1, Gulfam Ali Mughal1, Asad Ali Khaskheli1*, Turab Ali Korejo3, Nazeer Ahmed Rajput2, Qurban Ali Memon3 and Maqsood Ahmed Kumbhar4
Influence of Camellia sinensis on Broilers Performance
...(13.53%), liver (61.1%), spleen (2.26%), proventiculus (58.13%), intestine (102.2%) and fat pad (81.41%) were found in D group (control) and minimum in C group. Mortality percent was equally higher in A group (2%), B (2%) and D (2%) compared to C (1%). Considerable (P < 0.05) variation was seen among A, B, C and D groups for feed and water intake. Prominent (P < 0.05) variation was also noticed for carcass weight of birds in D group compared to A, B grou...
Muhammad Faisal Ayoob1,2, Zaheer Ahmed Nizamani1, Asghar Ali Kamboh3, Mansoor Ayoob4, Waseem Ali Vistro3 and Abdul Sattar Baloch3*
...morrhages in trachea and spleen. Histopathological lesions observed in proventriculus were erosions in epithelium, localized hemorrhages and congestion in the glandular region. In small intestine of affected birds, villi appeared hyperemic and ulcerated. Tracheal surface epithelium often showed discontinuation, the loss of cilia of the lining epithelium and disturbance of the coherence among various histological layers. In spleen
Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu*
... Biochemical parameters, spleen weight, physiological parameters, interleukin (IL-1β and IL-6) parameters and immunoglobin-E (IgE) were calculated at the end of the experimental study. To investigate the potential mechanism of action, Paw edema and mast cell de-granulation are estimated. Used to measure immune and inflammatory mediators, qRT-PCR technique. Quercetin significantly (P<0.001) reduced IgE levels in the asthma mice serum. Inflammatory cells...
Wentao Wang1,2, Xu Lin3, Jianshu Zhuo3, Dongjie Zhang2, Xiuqin Yang3* and Di Liu1, 2*
...udied with high level in spleen and muscle. Both of isoforms V1 and 2, containing functional domains of E2Fs, were localized throughout cells. No functional nuclear localization sequence and export signal were characterized through site-directed mutagenesis analysis, although their existence was predicted by bioinformatic methods. The results increase our knowledge of E2F3b mRNA diversity and provide basis for in-depth functional research.
...
Saqib Nawaz1, Sher Bahadar Khan1*, Umar Sadique1, Muhammad Israr2,
Mumtaz Ali Khan3, Hameed Ullah Khan4, Muhammad Irshad5, Ihsan Ali1 and Haq Aman Ullah1
..., nephritis 23 (46%) and spleenitis 13 (26%). Microscopic lesions revealed a score of 10 of kidney tissue out of 15 while the lesion score of intestine revealed 08 out of lesion score of 15.
... 
Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1
Ahmed Ali Moryani1, Nasir Rajput1*, Muhammad Naeem1, Atta Hussain Shah1 and Hidayatullah Soomro2
...organs; liver, pancreas, spleen, heart, duodenum, jejunum, ileum, thymus and ceaca was observed significant (P < 0.05) in the herbs supplementary groups as compared to control group. The compound-II supplementary group F significantly (P < 0.05) increased the percentage of crude protein and fat contents as compared to control and other groups. Histomorphological examination of liver of broiler chicken supplemented with Compound-I and Compound-II in the c...
Rajesh Kumar Oad1, Nasir Rajput1, Imdad Hussain laghari1, Hidayatullah Soomro1, Muhammad Azhar Memon2, Abdul Sattar Baloch5, Qudratullah Kalwar3, Mashhood Ahmed1, Zubair Ahmed Soomro2 and Waseem Ali Vistro4*
...n other groups, whereas, spleen and crop were heavier in group A, and gall bladder was heavier in group C. Mortality ratio increased in group A (p>0.05). Group E broiler spent more time on feeding (8.31 min/h). Group F spent more time on drinking (4.76 min/h). Group A spent more time on standing/walking (13.38 min/h). Group A spent more time on lying/sleeping (37.37 min/h) than other groups. Hematological observations indicated that the average value for RB...
Muhammad Khubaib Hameed, Muhammad Aziz ur Rahman, Muhammad Ashraf, Safdar Hassan, Muhammad Riaz, Muhammad Qamar Bilal, Fawwad Ahmad, Muhammad Sharif*
...ne organs such as bursa, spleen and thymus weight were also recorded. Weekly feed intake (3506g, 3531g) and body weight (2080g, 2139g) were better (P<0.05) in group fed arginine supplementation as 0.4 and 0.6%, respectively as compared to control group. Better feed conversion ratio (FCR, P<0.05) was shown by the all arginine treated groups with best FCR result in D group. Birds fed diet containing higher level of arginine (0.6%) had higher (P<0.05) dr...
Amira Mahmoud Refaie1*, Marwa Hosni Abd El-Maged2, Hassan Abd El-Kriam Hassan Abd El-Halim2, Hanan Abd El-Rahman Hassan Alghonimy2, Sayed Ahmed Mohamed Shaban1
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
...d nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphoma, the H and E, and immunohistochemistry were done. The immunohistochemical test exposed the immunophenotype positive for...
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
...d nodular lesions in the spleen and enlarged lymph nodes in the abdominal cavity. Subsequent cytological examination of smear made from lymph node FNA and peritoneal fluid revealed a large number of lymphoblasts. The above symptoms could be combined to make a preliminary diagnosis of lymphoma in dogs. To further determine the type of lymphoma, the H and E, and immunohistochemistry were done. The immunohistochemical test exposed the immunophenotype positive for...
Syukriah Syukriah1, Ulinnuha Nur Faizah2, Hendry T.S. Saragih3*, Rizki Fitrawan Yuneldi4, Soenarwan Hery Poerwanto5, Raden Roro Upiek Ngesti Wibawaning Astuti5, Stephan Immenschuh6
...spring’s liver and spleen were observed by using the TBARS Assay Kit, also the superoxide dismutase activities. As supporting data the histological analysis of the mother’s and offspring’s liver and spleen were prepared by using the paraffin method. There were sigificant results in the parasites levels and the increase in P. berghei infection followed an increase of oxidative stress in mothers and offspring...
Fatma Desouki Mohammed Abdallah
...art, liver, gizzard, and spleen) weight. SPSS packages used for calculation descriptive statistics, correlations and principal component reduction method. The results showed that Bartletts test of sphericity is highly significant (P = 0.000 **) for the three lines. Three principle components were able to explain 82.193% (53.927, 15.188, 13.078 for PC1, PC2, PC3 respectively) of the total variance in the eight variables of the high body weight line, two princip...
Samah Samir, Amal Awad*, Gamal Younis
...kidneys, gills, skin and spleen were examined bacteriologically for the presence of E. tarda; and confirmed by PCR targeting gyrB1 gene. Disk diffusion was used to test their antimicrobial susceptibility, and PCR was used to screen E. tarda isolates for the presence of four virulence-related genes (cds1, edwI, qseC, pvsA). In addition, the ability of E.tarda to form biofilm was tested by tube test. E.tarda was detected in 15 fish (6%) and a total of 40 isolate...
A. Samy1*, H.M.A. Hassan1, Fatma T.F. Abd-El Ghany2, Shama H. Morsy2
...rt, kidney, giblets, and spleen percentage were not affected. When curcumin or garlic extract were added at 200 or 400 ppm, Improved the antioxidants status by enhanced total antioxidant capacity also, significantly (P<0.05) enhanced hepatic antioxidants enzyme (superoxide dismutase, catalase, and glutathione peroxidase) compared with the control. Slightly (P>0.05) decreased in malondialdehyde of all treatments compared to the control. Also, there is no ...
Ahmed Alazzouni1, Ashraf Al-Brakati2,*, Sherif Rabie1, Mohamed Gabry1 and Basmaa Hassan1
...c lymph nodes (MLNs) and spleen. In this study 70 rats were divided into seven groups as follow: control, benzene (0.2 ml twice/week), Moringa oleifera (100 mg/kg), curcumin (300 mg/kg), green tea (350 mg/kg), combined green tea and curcumin, and cyclophosphamide (7.5 mg/kg) groups. All groups were treated for one month after the induction of leukemia. We found that the extracts ameliorated most histopathological changes in MLNs and sp...
Hany M.R. Elsherif1, Ahmed Orabi2, Hussein M.A. Hassan3, Ahmed Samy3*
...ver, heart, gizzard, and spleen were unchanged (% of body weight). Birds fed diets enriched with SF or SP had considerably (P<0.05) longer and wider guts than the control group, and scored zero in intestinal lesions scale. The addition of SF or SP were significantly enhanced (P<0.05) the lactobacillus count when compared to the control, while the quantity of Clostridium perfringens intestinal colonization was not exist. When OAS’s were introduced t...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
... brain, congested liver, spleen, brain and muscles besides greenish content of the gizzard. The histopathological examination of lung, brain, liver, and trachea of these diseased quails revealed non-suppurative encephalitis, perivascular cuffing, congestion of hepatic sinusoids, degenerative changes in the hepatic cells and interstitial lymphocytic aggregations in the liver and focal pneumonic areas in the lung. Pooled samples from the investigated quails were...
Luu Huynh Anh1, Huynh Tan Loc2, Nguyen Hong Xuan3, Le Minh Thanh1, Trinh Thi Hong Mo4, Ly Thi Thu Lan5, Nguyen Trong Ngu1*
...in the heart, liver, and spleen of chickens treated with MHH6 and PR2 phages were greatly decreased in infected chickens. After 98 days, the body weight of birds from the E. coli-infected groups treated with MHH6 and PR2 phages was lower than individuals in non-E. coli-infected groups (1,313-1,324 g/bird) but higher and significantly different from those in the PC group (1,172 g/bird). The majority of commercially important traits in chickens improved after ph...
Omnia Mohamed Khattab1*, Hala Kamel Abdelmegeed2, Mohamed Mahmoud Mashaly1, Mervat Hamdy1*, Naglaa Hagag1, Ayman Hamed3, Hanan Aly Fahmy3, Essam Ibrahim4, Momtaz Abdelhady Shahein2, Elsayyad Mohamed Ahmed2
...presence of 16 liver and spleen samples out of 20 (80%) from aborted fetuses were positive for EHV-4, but all were negative for EHV-1. Virus isolation trial for EHV-4 were done, eleven samples out of 20 (55%) on the CAM of ECE were positive. The virus glycoprotein B (gB) fragment (580pb) was amplified in selected isolates using the nested PCR (n-PCR), sanger sequencing of gB from three isolates with phylogenetic analysis reveals the full identity between the E...
Rijumoni Daimari, Silistina Narzari, Jatin Sarmah*
...a namely, liver, kidney, spleen, heart, large intestine and small intestine of Doom pig of Assam, India. The samples were collected from semi-extensively reared pig farms of Kokrajhar district, Assam. The approximate composition range of these edible viscera was found to be: moisture 50.67-68.47%, fat 0.47-9.85%, ash 0.16-0.92%, protein 6.85-22.36%, carbohydrate 10.18-24.65% and calorie 124.31-198.16 k/cal/100g. Among the samples the maximum protein, carbohydr...
Rezqita Putri Pitaloka1, Kartiawati Alipin1, Mas Rizky A.A Syamsunarno2, Gemilang Lara Utama3, Ramdan Panigoro2, Ratu Safitri1*
...the serum, liver, heart, spleen, kidney, and brain. The results showed that the effective dose was the combination of 75 mg/kg BW DFP and 50 mg/kg BW SWEE as an adjuvant, while 200mg/kg BW SWEE can be used as a substitute for chelators, especially in the heart. The decrease in serum and organ iron levels due to the combination of DFP and SWEE showed their ability to act as an adjuvant and substitute for chelators.
Keywords | Adjuvant, Deferiprone, Iron...
Xuan Liang, Rongfang Zhang, Yan Xu, Hui Yang, Li Guo, Feiyang Na and Donghai Liu*
...nvestigate the effect of spleen aminopeptide combined with xinxibao on erythrocyte immunity and Toll-like receptors (TLRs) signaling pathways in adjuvant treatment of children with recurrent respiratory tract infections (RRTI). A total of 138 children with RRTI admitted to our hospital from May 2017 to May 2019 were selected as the study subjects and randomly divided into 3 groups with 46 cases in each group. The 3 groups were given conventional treatment. On ...
Esam A Razin1, Hassan Sobhy2, Tarek R. AboElnaga1, Asmaa A. Darwish1*, Rasha S. Mohammed1
...er, kidney, heart, lung, spleen, and brain on the 49th day, stained, and histopathologically examined. Although, both treatments presented a significant improvement (P<0.05) in their parasitological and clinicopathological results, DAG suffered from lower levels of GR than BG, and its organs microscopical examination clarified severe lesions similar to TG. While, BG clinicopathological parameters and histopathological results were so close to CG. CK, CK-MB,...
Ahmed Ali Chandio1, Asghar Ali Kamboh1*, Nazar Ali Korejo2, Riaz Ahmed Leghari3 and Muhammad Ali Chandio4
... lymphoid organs (bursa, spleen and thymus) and enumeration of gut microflora. Results showed that PPLP improved (P < 0.05) production performance, final body weight, carcass weight and relative weight of lymphoid organs. Anti-NDV titer was improved on both 21 (P > 0.05) and 35 (P < 0.05) day in PPLP supplemented groups as compared to control. Total bacterial count and Lactobacillus count was increased (P < 0.05), while coliform count was decreased...
Heavy metals (HMs) are harmful and lethal at negligible levels and non-biodegradable in the typical ecosystem and constitutes animal, human and environmental hazards. They are divided into toxic metals like Lead, Cadmium, Arsenic, etc. and essential elements like copper, zinc, manganese, iron, nickel and chromium. Additionally, could be categorized into two groups based on the natural and anthropogenic sources releasing origins. Population and industrial expansion led to food contamination with HMs. Poisonous metals can be transferred from irrigation water to agricultural soils, agricultural operations, air pollution, animal feed, and packaging materials. Toxic metals are non-biodegradable, non-thermos degradable, and exceedingly stable in the ecosystem; as a result, they quickly build in various foods. Metal pollution of many foods, including agricultural commodities, and animal protein sources such as fish, milk, meat, and eggs, poses a hazard to food safety and security. Toxic metal pollution of irrigation water, agricultural soils, plants, and animals result in their integration into the food chain, posing a health hazard to humans. Most metals are harmful to animals and humans and accumulate in several organs like the skeleton, hepatic tissue, spleen, and renal tissues. Metals have a deleterious impact on the production of plants and animals. As a result, several remediation strategies have become necessary to limit the hazardous HMs pathway into the food chain and the human body. Metal nanoparticles are employed in beneficial applications, although they are associated with specific hazards.
Keywords | Food contamination, Heavy metals, Nanoparticles, Pollution sources, Remedy, Soil contamination
...keleton, hepatic tissue, spleen, and renal tissues. Metals have a deleterious impact on the production of plants and animals. As a result, several remediation strategies have become necessary to limit the hazardous HMs pathway into the food chain and the human body. Metal nanoparticles are employed in beneficial applications, although they are associated with specific hazards.
Keywords | Food contamination, Heavy metals, Nanopart...
Muhammad Arshad Iqbal*, Aamir Ghafoor, Irfan Ahmad, Muhammad Ijaz
...d at d35 and d42, liver, spleen, intestine, and ceca were collected to observe organ colonization. Use of Bacillus amyloliquefaciens as feed additive decreased the shedding pattern of S. Enteritidis and Cl. Perfringens and may pave way for safe poultry rearing and addressing public health concerns associated with AGP.
...
...
Abdulaziz A. Alaqil
...tive to the group C. The spleen, thymus, and bursa indexes as relative weights of FBW were significantly (p < 0.05) decreased by approximately 16%, 20%, and 35%, respectively, due to EC. Other immunological aspects were significantly (p < 0.05) impaired by EC challenge, recording a reduction of 38% in the total white blood cells counts, 12% in the leukocyte cell viability, 19% in the wattle thickness against phytohemagglutinin injection, 44% in the anti-...
Abdulaziz A. Alaqil*
...tionally, samples of the spleen and duodenum were taken from layers in each group (n=10) to assess heat shock protein 70 (HSP70) density and glutathione peroxidase (GHS-px) activity, respectively. The general linear model procedure with one-way analysis of variance (GLM-ANOVA) was used to analyze the data. The results showed a significant (P<0.05) increase in the BT, H/L ratio, MDA, and CORT, and over-expression of HSP70 in the hens of LPS group. Also,...
Xiaowei Huang, Yajie Wang, Jia Zhou, Junxiu Liu, Zhe Lin and Ruili Li*
...he changes of thymus and spleen indexs were compared, the levels of white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb), hematocrit (HCT) and platelet count (PLT) in peripheral blood were detected, and the changes of hemorheology were observed. Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH) and reduced glutathione pero...
Mohammed A. El-Sayed1*, Mahmoud H Hatab2, Heba AEM Assi3, Nashaat S Ibrahim2, Hisham M Saleh2, Waheed AA Sayed2, Birgit A Rumpold4
...t, liver, gender organs, spleen, and bursa of Fabricius of quails when compared to diet A. The LBW, relative carcass weight, and measured organs of the diet A group were significantly impacted by the interaction of dietary treatment and HS, whereas these traits had no or minimal effects on the diet B and diet C groups. The HSP70 gene transcription level was highest in birds exposed to cyclic HS and fed control diet A, and lowest in birds in the diet C groups. ...
Dhurgham K. Seger1, Rahman H. H. Al Qasimi2, Azhar A. Jaffar3, Salah H. Faraj4, Ahmed I. Ateya5*
...learance percentage, and spleen and kidneys weights. This study highlights the possible efficiency of using SSTR1 as candidate marker for carcass traits in sheep. ...
Zhipeng Song1,2, Jialiang Xin1,3, Xiaoli Wei1,2, Abula Zulipiya1,3, Kadier Kedireya1,2 and Xinmin Mao1,3*
...F, PI3K, fibrin (FN) and spleen tyrosine kinase (SYK), and upregulating the level of E-cadherin, an epithelial marker. Molecular docking results showed that marein could integrate with VEGFA, PI3K and SYK, and the resulting conjugate was more potent than the positive drug metformin. Among the above factors, the combination effect of SYK and marein was the most ideal. To conclude marein can improve glucose and lipid metabolism disorders in db/db mice after a tr...
Muhammad Shahid Nisar1*, Ahmad Kamran Khan1, Ghulam Fatima2, Irfan Ahmed4, Khizar Sami Ullah2 and Syeda Amber Hameed2,5
...estine, pancreas, lungs, spleen and kidney) resulted significantly difference (p<0.05) and affected with FW supplementation. Broiler fed with 25% and 50% FW affected positively (p<0.05) to lymphocytes, granulocytes, red blood cells, hemoglobin, mean corpuscular hemoglobin and mean platelet volume while D3 also resulted positively in results of mid-range absolute count. While birds fed D4 (75% FW) resulted significant difference (p<0.05) in mean platel...
Promising Immunomodulation Potential of Ganoderma lucidium and Oregano as Mixture on Broiler Chicken
Fateh A. Badi*, Anwar A. G. Al-Kubati, Naif Al-Gabri
...yte ratio, bursa index, spleen index, bursa to spleen ratio and phagocytosis index using carbon clearance test. This study showed that haematological parameters (haematocrit, Hb, RBC, MCV, MHC and MHCH) were all within the normal range values in all the groups. The mixture of plant and mashroom insignificantly increased the total WBCs compared with G group. Mixture and Ganoderma groups was significantly decreased the heterp...
Franciscus Rudi Prasetyo Hantoro1,2,*, Dwi Sunarti1, Turrini Yudiarti1, Sri Sumarsih1, Rini Nurhayati2
... the bursa fabricius and spleen, levels of malondialdehyde (MDA), number of Newcastle Disease Virus (NDV) antibodies, increase in body weight, and feed efficiency. High stocking density has a significant impact on increasing the number of LAB and coliforms in the ileum, reducing the weight of the thymus, and reducing the amount of feed consumed. The lower the crude protein level, the lower the NDV antibody titres, but it did not affect the blood profile, LAB a...
Ahmed Abd El Samie Hassan Ali
...hen tissue harvests from spleen, lung, lymph nodes and intestine were pooled, homogenized; the supernatant was clarified and concentrated for second inoculation. A another 16 BVDV seronegative New Zeeland rabbits, classified into 4 groups, each of 4 designated for second closed inoculation with clarified supernatant of rabbit tissue harvests of first inoculation in comparison to reference BVDV type-I-NADL, BVDV type-2-890 and control negative. Clinical scores ...
Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy
...of the white pulp of the spleen as well as edema in the lymph node. The brain of inculcated mice showed intracytoplasmic inclusion body. Electron microscopy (EM) were applied on brain of infected mice and showed intracytoplasmic small number of bullet shape particles.
Virological and pathological results were discussed.
...
Farid S. Nassar1,2, Osama A. El-Sayed3, Saidi Ouassaf4, Ahmed O. Abbas1,2*
...ield, breast, liver, and spleen increased substantially (p < 0.05). The plasma total protein and triiodothyronine hormone levels were remarkably (p < 0.05) enhanced by adding FS to broiler meals. At the same time, the concentrations of alanine transferase, aspartate transferase, and uric acid were significantly (p < 0.05) lowered. Additionally, the triglycerides were dramatically (p < 0.05) decreased while the high-density lipoprotein composed a hi...
Sajjad Khan*, Naila Chand, Abdul Hafeez and Nazir Ahmad
...izzard, pancreas, bursa, spleen and thymus was recorded for the groups studied. Significantly higher (P<0.05) VH, lower (P<0.05) CD and higher (P<0.05) VH:CD in duodenum, jejunum and ileum were recorded in synbiotic group D followed by C, E and B. Similarly, supplementation of synbiotic in group D and Bacillus subtilis group C resulted in complete eradication of E. coli, Salmonella and C. perfringens from ileum, caecum and colon of experimental broile...
Faramin Javandel Soum Sarai1, Mir Daryoush Shakouri2* and Alireza Seidavi3*
...eters, abdominal fat and spleen weights (P<0.05). The results suggest that supplemental chromium picolinate alleviate the adverse effects of heat stress. No synergistic effect was observed between vitamin C or formic acid with chromium picolinate, and in some cases formic acid exacerbated the effect of chromium picolinate....
Sijie Jian1,2,3,4, Wei Sun1,3, Jia Chao1,2, Na Rong1,4, Xiang Liu1,2,3,4* and Chen Chen1,2,3,4*
...lt; 0.05) in kidneys and spleens after challenging with A. hydrophila and P. fluorescens; and reduced injury to the kidneys, spleens and intestines; there was no significant change in the antioxidant-related factors of MDA, SOD, CAT and GSH-PX. Therefore, Slp has passive protection (A. hydrophila) and passive cross-protection (P. fluorescens) abilities and it can boost resistance to infection using major freshwater aquacultu...
Rahib Ali Chachar1, Asghar Ali Kamboh1* and Manatbai Bakhetgul2
...e relative weight of the spleen was found higher (p<0.05) in T2 and T3 groups as compared to T0 and T1 groups. The relative weight of the thymus was recorded as higher (p<0.05) in T3 as compared to control and other treated groups. The relative weight of the bursa was higher (p<0.05) in the T1 and T3 groups as compared to T0 and T2 groups. All treated groups exhibited significantly high (p < 0.01) total bacterial count, Lactobacillus and Bifidobact...
Philip O. Akporhuarho1, Ufuoma Godstime Sorhue1*, Adimabua Mike Moemeka2, Onyinye Stella Onwumere-Idolor3, Jonathan Ujomu1, Emmanuel Abadah1, Jennfer Aaron1
...kin-1β genes in the spleen of chickens subjected to Aframomum chrysanthum seed meal (ACSM) as a replacement for synthetic antibiotic in broiler production. A total of one hundred and forty-four day-old Aboracre strains of broilers were randomly allotted into four dietary treatments. Treatments one (control) had no ACSM in the diet but was given routine antibiotics medication for broilers. Treatments two (T2), three (T3), and four (T4) had ACSM at 1%, 1.5%...
Bushra AL-Khaqani, Amira Mohammed*
...sion of Il10 gene in the spleen, which is associated with T-regulatory and anti-inflammatory processes in the immune system. Our findings showed that RES may reduce asthma by activating anti-inflammatory genes, restoring lung barrier integrity, and decreasing proinflammatory cytokines and infiltrating inflammatory cells.
...
...
Muhammad Ilyas Riaz1, Masood Rabbani1*, Sohail Raza1, Ali Raza Awan2, Aleena Kokab1, and Rida Haroon Durrani1
...nd cleared from the mice spleen after 20th DPI when inoculated at the dose of 108 CFU per mice. In contrast to this, the parent RB51 strain induces splenomegaly and showed higher persistence with significantly higher splenic CFUs in the mice group at 10th and 20th DPI as compared to B. abortus ΔpurD mutant. The histopathological comparison of spleens from both groups also revealed much infiltration of giant macrophages...
Md. Khayrul Basher1, Sumon Sarkar2,3, Md. Samiul Haque1, Sourav Sarker4, Md. Rashedul Islam1*
...body weight ratio in the spleen and slight discoloration and consistency of the liver and lung than the control group. It’s interesting to note that co-administration of sodium selenite markedly mitigated all the changes caused by arsenic-induced alteration of hematological parameters, serum biochemical parameters, organ to body weight ratio (p<0.05, p<0.01, p<0.05, respectively). The current study provides compelling evidence for the protective...
Muhammad Choirur Roziqin1, Muhammad Halim Natsir2*, Osfar Sjofjan2
...e liver, heart, gizzard, spleen, and pancreas, as well. However, the use of date pit flour in feed has a very real effect (P<0.01) on body weight gain, carcase percentage, abdominal fat, and cholesterol. In conclusion, date pit flour feed was recommended as an alternative feed for broiler chickens.
Keywords | Broiler chicken, Carcase, Internal organs, Growth performance, Date pit flour
...
Sura Ayed Radam*, Inam Badr Falih
...rplasia were reported in spleen of 1st group immunized with Streptococcus pyogenes lyophilized antigen. Conclusion This study provide we can conclude that jointly of concanavalin-A and S.pyogenes antigen capable to enhance potential immune response butter than antigen alone.
Keywords | Comparative study, Streptococcus pyogenes, Concanavalin-A, Lyophilized antigen, Immunized rats, Histopathological
Ayoola Mathew Oluwaseyi1*, Aderemi Foluke Abimbola1, Alikwe Philip2 , Tumgbulu Samuel2, Eniufuoma Augustina2
...) affected by ECLM while spleen and bursa of fabricius were not significantly (p>0.05) affected by ECLM feed additive. Measured serum total protein of the groups on ECLM were significantly (p<0.05) higher, while serum cholesterol were significantly (p<0.05) lower than those on control group. Birds on 1% and 2% ECLM inclusion had the best dressed weight which was significantly (p<0.05) higher than those on control. ECLM inclusion as feed additive in...
Talal Jabal Hussen1, Sattar J.J. Al-Shaeli1*, Baidaa H.R. Al-Mahna2, Hasanain A.J. Gharban3
... structure of the liver, spleen and kidney. An overall 40 BALB/c adult female mice were selected, acclimated, and divided randomly and equally into control (non-received estrogen) group and estrogen group. After 35 days of estrogen therapy, blood samples and tissue sections were collected from all study animals for biochemical assays and histology detection. The obtained data show that the biochemical markers displayed significant increased in estrogen adminis...
Mohamed Ahmed Abaza1, Amany O. Selim2, Mona Abdallah3, Shimaa A.E. Atwa4, Hala El Daous5, Mona Abd-Allah Abd-Elrehim6, Mohamed M.S. Gaballa7, Reda R. Fathy1*
...the liver, cecum, colon, spleen, heart, and lungs compared to the E. coli-infected group. These findings solidified our central hypothesis which was to evaluate the antimicrobial and antioxidant efficacy of ZnO-NPs against pathogenic bacterial strain of E. coli in broiler chicken as a powerful, safe alternative to antibiotics.
Keywords: Nano-production of zinc oxide (ZnO-NPs), Specific pathogen free c...
Dalia Hamid Mansour1, Mohamed Elshabrawy Ghanem2, Marwa Hassan3, Yousry Ibrahim4, Ibrahim M. Hegab5*
Nahed Yehia1*, Rania I. Mohamed2
...roventriculus. Also, the spleen showed severe lymphoid depletion and necrosis causing immunosuppression. Regular virus monitoring and study of the pathogenicity is necessary for better control of virus infection.
Keywords: H9N2, Genetic evolution, Pathogenicity, Histopathology, Avian influenza
...
Mohamed T. A. Soliman1, Hadeer M. Shosha2, Hala M. Ebaid2, Heba Nageh Gad El-Hak2, Heba M.A. Abdelrazek3*
...(TOS) were assessed. The spleen and thymus were preserved for histopathological and ultra structural analysis. The male offsprings, who were exposed to low and high COC levels throughout their mothers’ pregnancy, statistically P≤0.05 exhibited a significant elevation in IL-2, IL-6, IgG, IgE and TOS levels in comparison to the control rats. On the opposite hand, IL-4 and TAC showed reduction in their level. Rat pups of the mothers that were given 73.5m...
Mat Zin Ain-Auzureen1, Maizan Mohamed1, Tan Li Peng1, Mohd Faizal Ghazali2, Choong Siew Shean1, Kamaruddin Mardhiah3, Najiah Musa4, Nora Faten Afifah Mohamad5, Chai Min Hian2 and Ruhil Hayati Hamdan1*
... pale liver and enlarged spleen. Twenty-six isolates of V. alginolyticus were containing chiA (100%) and 96% of colA genes. None of the isolates possess hlyA, toxRVH, toxRVC, and trh-tdh genes. MVGi were varying from 0.16 to 0.33. In addition, 18 antibiotic resistance patterns were identified, and the MARi ranged from 0.05 to 1. It is alarming that seven out of 12 isolates from Sungai Besut...
Sherine Abbas1, Heba M.A. Abdelrazek2*, Eman M. Abouelhassan3, Nayrouz A. Attia4, Haneen M. Abdelnabi4, Seif El-Eslam E. Salah4, Abdelrahman M. Zaki4, Mohamed A. Abo- Zaid4, Nadia A. El-Fahla5
...) and histopathology for spleen and thymus were performed. Oral administration of neem extract to pregnant female rats caused significant (P<0.05) declines in birth and final body weights of male offspring and their thymus absolute weight (P<0.05). It also caused significant neutrophil (%) promotion (P<0.05) and lymphocyte (%) reduction (P<0.05), reduced serum TAC levels statistically (P<0.05), and affected DNA and lymphoid tissues evidenced by ...
Hakeem Jawad Kadhim1, Abbas Kamil Shlaga1*, Safaa Hussein Ali2
...lesions, such as mottled spleen and hemorrhages in the proventriculus and cecum. The NDV infection was confirmed using the NDV rapid test, which showed positive results in 52 out of 60 suspected samples (86.66%). Furthermore, RT-PCR data revealed that only 27 (51.92%) out of 52 samples were positive for the M gene. Thereafter, the mean antibody levels of ND-infected birds were significantly lower than those of uninfected birds. In contrast, inflammatory biomar...
Syed Abdul Hadi1, Jameel Ahmed Gandahi1*, Muhammad Ghiasuddin Shah1, Saima Masood2, Noor Samad Gandahi1
...red to PC. The weight of spleen, heart, and kidney was unaffected (P≥0.05), while weight of liver significantly increased (P≤0.05) in HZNC compared to PC. In conclusion, the combined supplementation of ZnO-NP 60 mg/kg and COS 200 mg/kg effectively mitigated the negative consequences of heat stress thus could be adopted in hot climates as feed supplement instead of synthetic antibiotics. ...
