Asma Noshad1, Mudassar Iqbal1*, Zafar Iqbal1, Hamida Bibi2, Saifullah3, Salma Bibi4, Hamid Ullah Shah1
...ruit bodies, mycelia and fermentation filtrate obtained from Pleurotus ostreatus also known as the oyster mushroom. The percent mortality of Macrosiphum rosae (rose aphids) and the LC50 values were calculated and used as an indicative of insecticidal potential. The maximum mortality of aphids was achieved at 80 µg mL-1 and the LC50 for the fruit body extract after 24 HRS was calculated as 12.83μg mL-1, follo...
Ali Muhammad*, Muhammad Ayub
...ng brine treatment, fermentation take place which significantly convert the oleourepean into simple sugar and decreased during treatment process. Lye solution penetrates inside the msesocarp and than endocarp during four weeks of treatment process. The lye treated olive fruits were treated afterwards into brine solution for the next week and so on which gradually remove the bitter taste of lye solution and got salted taste during four ...
Nighat Sultana1,2,*, Shazia Iftikhar1, Nafeesa Qudsia Hanif2 and Iffat Tahira2
...ize fodder using a model fermentation system and raw materials from different areas of Punjab, Pakistan. A. niger, A. flavus and A. fumigatus were the most dominant followed by A. terrus and A. ochraceous in fresh and ensiled maize fodder. Total fungal counts ranged from 1×103 to 4×103cfu/ml for all species in both fresh and ensiled maize fodder. Total aflatoxins (AFB1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA) analysis showed that AFB1was presen...
B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran
Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...a spp. viz., solid state fermentation and liquid state fermentation. In solid fermentation, the fungus is grown on various cereal grains, agricultural wastes and byproducts. The solid state production is highly labour intensive and fit for cottage industry. These products are used mainly for direct soil application in nurseries/main fields to suppress the soil-borne inoculum. In liquid sta...
Mutassim M. Abdelrahman1*,Ibrahim Alhidary1, Abdullah H. Alyemni2, Rifat U. Khan3, Abdel Raouf S. Bello4, Mohamed Y. Al-Saiady2 and Ramzi A. Amran1
...nificant change in rumen fermentation patterns as a result of increasing the intake level of NDF from the alfalfa hay. This shift in the fermentation pattern may positively affect the rumen environment and consequently growing lambs’ health and productivity.
...
Rahat Naseer1*, Abu Saeed Hashmi1, Zulfiqar-ul-Hassan2, H. Rehman1, Saima Naveed1, F. Masood1 and M. Tayyab1
...ali, water treatment and fermentation to prepare processed moiety on pilot scale. All these processed materials were included in experimental ration at a fixed level (20 %). All iso-caloric and iso-nitrogenous rations were offered to experimental animals (n=36, 22 male 14 female Lohi sheep almost evenly distributed in experimental groups) having almost uniform body weight (BW). During 60 days of the trial, no significant difference (p =0.067) in the daily feed...
Ibrahim A. Alhidary1,*, Mutassim M. Abdelrahman1, Riyadh S. Aljumaah1, Abdullah H. Alyemni2, Moez A. Ayadi1,3 and Mohamed Y. Al-Saiady2
...aracteristics, microbial fermentation and growth performance.
...
Jawad Anwar* and Zafar Iqbal
... media of pH 5 and light fermentation.The EtOAc extract exhibited the minimal value of MIC (10.41±4.50 µg/mL) against gram negative E. coli, while its acetonitrile fraction yielded (6.50±2.25 µg/mL) of MIC against S. aureus. Hence, it is revealed from the results that T. harzianumis a potential source of antibiotic drugs as it showed a wide range of bioactivity against both phyto and human pathogenic bacteria. ...
Ehab Bo Trabi1,2, Xianjun Yuan1, Junfeng Li1, Zhihao Dong1, Assar Ali Shah2 and Tao Shao1,2,*
.../div>...
Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1
...oculum size in submerged fermentation for 24h. Results showed that among these two treatments, only chemical treated substrates gave better enzyme yield as compared to chemical followed by steam. The highest FPase (2.526 IU/ml/min) activity was found at pretreatment conditions of 0.6% NaOH conc., 10% biomass loading and 4h reaction time while CMCase (2.803 IU/ml/min) at 1% NaOH conc., 15% biomass loading and 6h of reaction time. The proposed model was found hi...
Fatima Arshad1, Muhammad Irfan2*, Hafiz Abdullah Shakir3, Fouzia Tabbsum3, Javed Iqbal Qazi3
...btilis K-18 in submerged fermentation at 50 °C for 24 h. Results revealed that acid pretreatment was found more effective for cellulase production as compared to thermochemical pretreatment. The maximum CMCase activity was 1.757 1U/ml/min under conditions of 0.6% acid concentration, 10% substrate concentration, and resident time of 4h. The maximum FPase activity was 2.015 1U/ml/min under conditions of 0.8% acid concentration, 10% substrate concentration, a...
Bushra Mazhar1, Nusrat Jahan1, Nazish Mazhar Ali1, Saiqa andleeb2, Shaukat Ali2*
... conditions and suitable fermentation medium, E. hormaechei can be used at an industrial level as producer of vanillin. Bioconversion of waste residues of rice bran oil to vanillin by bacterial strains is of greater interest nowadays. Bioproduction of vanillin is not only considered natural but also safe for human health....
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
...ncluded CSL 6%, 24 h. of fermentation, pH 7.5, 37oC, KH2PO4 0.04%, K2HPO4, NaHCO3 0.15% and 0.015% CH3COONa. The results indicated that UV is the most effective mutagen for hyper-production of SK. Mutant strain UV6 has the potential for enhanced SK production with commercial applications.
...
Xiaopeng Tang1,3, Rong Xiang1, Sijia Chen1,3, Shufen Yang1,3, Hu Liu1,3, Rejun Fang1,3,* and Aike Li2,*
... to study the effects of fermentation and enzymatic hydrolysis treatment of cottonseed meal on crude protein (CP), water-soluble protein (WSP), amino acid (AA) and peptide fractions, and the AA digestibility and metabolic energy of fermented cottonseed meal (FCSM) and enzymatic hydrolyzed cotton seed meal (EHCSM) in white leghorn roosters. Firstly, CSM were fermented with Aspergillus niger, or hydrolyzed with alcalase and flavourzyme. Secondly, a total ...
Partial Characterization of α-amylase Produced from Aspergillus niger using Potato Peel as Substrate
Shahzad Mahmood1, Memuna Ghafoor Shahid1, Muhammad Irfan2, Muhammad Nadeem3*, Quratulain Syed3
... this study, solid state fermentation of potato peels were done by Aspergillus niger for the production of α–amylase. The crude enzyme produced was further characterized. The results showed that enzyme had an optimum pH of 6.0 and stable in range of 4.0-6.0. Optimum temperature of enzyme was 40oC and attained 96% activity at 40oC for 70min. Metal profile showed that α-amylase activity was enhanced in the pres...
Noor Fatima1, Muhammad Irfan2,*, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1
... carried out for 24 h of fermentation period at temperature of 50oC. Results showed that cellulase production was greatly affected by the thermochemical pretreatment as compared to the chemical pretreatment. At the pretreatment condition of 1% H2SO4 conc, 6h residence time, 15% substrate concentration maximum CMCase production (0.858 IU/ml/min) was obtained while at pretreatment conditions of 1% H2SO4 conc...
Jun Chen1, Shuai Shang2, Xiaoyang Wu1, Jiakuo Yan1, Huaming Zhong1, Huanxin Zhang2, Weilai Sha1, Wanchao Zhu1 and Honghai Zhang1,*
...terial through microbial fermentation in the hindgut. So far, there has been no study of the gut microbiota of the cape oryx. Here, we provided the first description of the fecal bacterial populations of the cape oryx by using high-throughput Illumina sequencing technology. We analyzed 100,180 high-quality sequences of the 16S rRNA gene obtained from fecal samples from three cape oryx animals, one female and two males. At the 3% level in our research, we found...
Changqing Zhao*, Zhuochi Chen and Yubin Li
...oteases) produced during fermentation, and increased the amount of free amino acids and flavored compounds. In these ways, the meat texture and taste were improved, and digestion and absorption were enhanced.
...
Xiaqing Chen, Yü Huang and Zhen Huang*
...ruits. T. hamatum fermentation broth and its ethyl acetate extracts caused high mycelial growth inhibition and germination inhibition of S. shiraiana, with the inhibition value up to 80% and 90%, respectively. An apparent increase in mycelial growth inhibition in a dose-dependent manner was observed when ethyl acetate extract and carbendazim were applied individually. The EC50 of T. hamatum ethylacetate extract or carbendazim in...
Ammara Afzal1, Tazmeen Fatima1, Maham Tabassum1, Muhammad Nadeem2*, Muhammad Irfan3, Quratulain Syed2
... separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) process. Fungal strains of Saccharomyces cervisae, Trichoderma viride, Trichoderma koninji and Trichoderma harzianum was used for subsequent process using saw dust as substrate. Three major processes convert lignocelluloses to bioethanol i.e. pretreatment of biomass, enzymatic hydrolysis of raw material a...
Tahira Jamil*1, Mohammad Asghar1, Fatma Husain1, Haq Nawaz Bhatti2
..., pH, inoculums size and fermentation time (each at five levels) were optimized using central composite design (CCD) design in 30 triplicate experimental runs. The maximum statin formation (19 mg/g) was achieved at 25oC, pH 5.5, inoculums size 3.5 mL and fermentation time 144 h on wheat straw as a growth substrate. The growth medium has an important effect on lovastatin production. Effect of carbon and nitrogen su...
*Irum Javed1, Muhammad Asgher2, Sadia Noreen1, Humara Naz Majeed1 and Tanzila Sahar1
SOLID STATE FERMENTATION OF ASPERGILLUS NIGER FOR CITRIC ACID PRODUCTION USING AGRICULTURE RESIDUE AS SUBSTRATE
...lled by biotechnological fermentation using agro-industrial wastes. It is a bidirectional beneficial process for cost effective manufacturing of citric acid and management of agro-industrial waste products. This project was carried out in order to evaluate the enhanced production of citric acid by Aspergillus niger using agro-industrial wastes. In the first step of study the liquid state fermentation (LSF) and solid state
Haniya Mazhar1, Naaz Abbas2, Zahid Hussain3, Amir Sohail4, Syed Shahid Ali4*
Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels
...f lipases by solid-state fermentation (SSF) using agro-industrial wastes as substrate. A total of 14 bacterial strains were isolated, screened and compared for lipase production. Among the evaluated strains, Bacillus subtilis strain NL-39 was selected on the basis of best lipase production. The maximum enzyme activity (34.93 U/ml) was shown by B. subtilis using SSF with soya bean meal. The lipase production by other low cost agro-industrial waste, including su...
Mohsin Arshad1, Muhammad Irfan*2, Asad-Ur-Rehman1, Muhammad Nadeem3, Zile Huma3, Hafiz Abdullah Shakir4, Quratulain Syed3
Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification
...ated in submerged
fermentation using agricultural wastes as substrates for carboxymethyl cellulase
(CMCase) production under static and with agitation speed of 140 rpm. The whole
fermentation experiments were carried out in 250ml Erlenmeyer flask. Different
agricultural wastes such as sugarcane bagasse, wheat straw, rice husk, defatted
soybean meal, corn cobs and wheat bran were exploited f...
fermentation using agricultural wastes as substrates for carboxymethyl cellulase
(CMCase) production under static and with agitation speed of 140 rpm. The whole
fermentation experiments were carried out in 250ml Erlenmeyer flask. Different
agricultural wastes such as sugarcane bagasse, wheat straw, rice husk, defatted
soybean meal, corn cobs and wheat bran were exploited f...
Raazia Kiran1, Alya Riaz1, Muhammad Irfan1*, Hafiz Abdullah Shakir2
An overview of pre-treatment methods used for bioconversion of lignocellulosic biomasses into valuable products
...eatment, hydrolysis, and fermentation.
The pre-treatment method involves the use of different physical processes i.e., size
reduction, steaming or boiling, ultrasonication, and bursting, chemical methods i.e.,
solvents, acids, salts, bases, the physicochemical processes include liquid hot
water and AFEX (ammonium fibre explosion), biological methods involving whiterot/
brown-rot fungi and bacteria, and quite a few combination...
The pre-treatment method involves the use of different physical processes i.e., size
reduction, steaming or boiling, ultrasonication, and bursting, chemical methods i.e.,
solvents, acids, salts, bases, the physicochemical processes include liquid hot
water and AFEX (ammonium fibre explosion), biological methods involving whiterot/
brown-rot fungi and bacteria, and quite a few combination...
Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2
Yang Liu1, Jing-xin Mao2, Xiao-dong Wei2, Man Yi2, Xiao-long Zhang2, Ke Zheng3, Xian-xin Chen4, Guo-Ze Wang5 and Bing-bo Chen1,*
... were increased in mixed fermentation BFA group significantly than the control group (P<0.05). For feed conversion rates in each group, the mixed fermentation BFA group had the highest feed efficiency, increased by 10.04% than the control group, followed by the yeast BFA group, with an increase by 4.14%. The mixed fermentation BFA group and lactobacillus ferment...
Sohaib Afzaal1, Usman Hameed2, Nasir Ahmad3,*, Naeem Rashid3 and Muhammad Saleem Haider1
...was carried out by sugar fermentation tests. These strains were further analyzed for their ability to produce lactic acid. Overall, Leuconostoc mesenteroides was found as pre-dominant group (41.1% prevalence) among LAB strains in the isolated food samples. Some opportunistic pathogens were also isolated from these media. Among the tested strains maximum amount of lactic acid (26.457 mg/mL) was produced by the lactobacilliafter 48 h growth in skim milk b...
Ghalia Nawal1, Muhammad Irfan2*, Saira Ashfaq3, Hafiz Abdullah Shakir1
...volves Sub-merged liquid fermentation in fed batch manner using agricultural wastes. Bacterial lipases are preferred due to their extra cellularity, easy step down approach and practicability of increased productions via technological modifications. They have wide- ranging applications in diverse sectors of the food, medical, cosmetic, detergent sectors and in paper industry which establishes their irrefutable noteworthiness.
... 
Azeem Haider1, Muhammad Mohsin Alam2*, Azhar Ali Khan3 and Muhammad Asif Zulfiqar3
...dicated that the optimum fermentation medium in shake flask studies contained a carbon (glucose 4%), nitrogen (0.5% ammonium chloride), inoculation level (three disks, 0.5 cm in diameter), temperature (30 °C), incubation period (at 7 days) and initial pH 5.0. Under these culture conditions, the maximum level of xylanase activity (9.29 Uml-1), CMCase (7.37 Uml-1), laccase (13.57 Uml-1) and effluent decolorization (69.68 %) were observed on 7th day during sh...
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *
... (P), potassium(K)in the fermentation process. Poultry litter, sawdust and rice husk were mixed in equal quantities by volume. One was treated with a single strain of Bacillus; another was treated with a consortium of bacteria comprising several species; and the third kept as the control without adding any bacteria. The results showed that decomposition started earlier in the treated batches than in the control group. During the
Muhammad Suleman1,*, Abu ul Hassan Faiz2, Muhammad Shahbaz2 and Ayesha Riaz3
...n of xylanase. Submerged fermentation was carried out in 500 ml Erlenmeyer flasks using vogel’s medium at 35°C for five days. UV irradiated A. niger has significantly increased the xylanase production as compared to parent strains. Twenty minutes exposure of A. niger was considered suitable for the production of xylanase. Wheat bran concentration (3.5%) was considered best substrate for xylanase production than corn cobs and sugarcane b...
Sohaib Afzaal1,4*, Usman Hameed2, Nasir Ahmad3, Klas Udekwu4, Paulina Pastuszek4 and Muhammad Saleem Haider1
...of these strains in food fermentations is future prospect of the study. ...
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
... Tautomycetin. Different fermentation parameters were optimized to produce antimicrobial agents using Response Surface Methodology (RSM) and best results were obtained at 45°C temperature, 5.5 pH, 5 mL inoculum concentration, 1.5 mL banana peel as substrate and 20 days incubation time. The antimicrobial activity of secondary metabolites was tested against Streptococcus aglactaie, Streptococcus pyogene, Escherichia coli and Prote...
Sobia Nazir1, Muhammad Irfan1*, Muhammad Nadeem2, Hafiz Abdullah Shakir3*, Muhammad Khan3, Shaukat Ali4, Quratulain Syed2
...roduction in solid state fermentation (SSF) using seed pods of Bombyx ceiba as substrate. Three parameters were used such as inoculum size (1, 5.5 and 10%), medium volume (1, 3 and 5ml) and incubation period (3, 4, 5 days) to observed the maximum activity of cellulase using Box-Bhenken design of response surface methodology. Maximum activity of CMCase (0.700 IU/gds/min) and FPase (0.260 IU/gds/min) was observed on 4th day with inoculum size o...
Awais Bin Shahid1, Moolchand Malhi1,*, Saeed Ahmed Soomro1, Muhammad Giasuddin Shah2, Nazeer Hussain Kalhoro3, Asmatullah Kaka4, Rajoo Mal1, Muhammad Awais Soomro1, Saba Parveen Samo1 and Muhammad Nawaz Sanjrani5
...enium yeast (SY) on fermentation pattern, papillae morphology and antioxidant status in rumen of goats. A total of ten goats were randomly divided into two groups; control (C, n=5) and selenium yeast (SY, n=5). Animals were fed concentrate (2 % BW) as basal diet without (C) or with selenium yeast (SY, @ 0.3 mg.Se.kg-1.diet) supplementation. Hay and water was provided ad libitum. The results revealed that the molar concentrat...
Elmas UlutaĹź1,*, Abdullah Eryavuz2, Aziz Bülbül2, Abdur Rahman3,*, Ä°smail Küçükkurt4 and Cangir Uyarlar5
...ining high zinc on rumen fermentation, blood parameters, live weight and oxidative status in goats. In this study, twenty four male Angora goats each of 12 months of age, and weighing approximately 35 kg were divided into four groups as: control group (C) fed with basal diet containing 31.76 ppm Zn, experimental group 1 fed with basal diet supplemented with 500 ppm Zn, experimental group 2 fed with basal diet supplemented with 750 ppm Zn and experimental group...
Xuhui Zhang1, Zhiyuan Sun2, Jinfeng Cai1, Guibin Wang1, Zunling Zhu1, Linguo Zhao3 and Fuliang Cao1,*
...FR) products produced by fermentation using Aspergillus niger on growth, antioxidant capacity and meat quality of broilers. Three hundred day-old commercial Arbor Acres (AA) broiler chicks were randomly allocated into 5 dietary treatments including five variations: control group, fed with the basal diets; NF group, the addition of 3 g/kg NF products; FR1, FR2 and FR3 groups, addition of FR products 1 g/kg, 3 g/kg and 6 g/kg, respectively. There was no d...
Renlong Lv1*, Ken-Ichi Horiguchi2, Shin-Ichi Tagawa3, Shoichi Ichihara4 and Norio Yoshida5
...er function, and ruminal fermentation in sheep fed heat-treated jatropha meal. The experiment was performed twice (experiment I and II). The subjects were 6 wethers. They were divided into two treatments (rapeseed diet and jatropha diet) randomly and were tested by using a cross over method. Rapeseed meal and jatropha meal were relaced to the feed as a concentrate with proportion of 5% in experiment I and 10% in experiment II. The ingestion rate of sheep fed t...
Sikander Ali* and S. Wajiha Khalid
...ty at pH 6 after 72 h of fermentation (158±0.39 U/ml). The partial purification of the enzyme was done with ammonium sulphate (20-80 %) followed by dialysis which resulted in 21 % purification from the crude enzyme extract. The molecular weight of enzyme was found to be 86 kDa, as determined by SDS poly-acrylamide gel electrophoresis.
... 
S. Ahmed† , L. Yue, Q. Liu and H. Jian
Evaluation of media for mass production and fermentation of Pseudomonas spp. for integrated management of cereal cyst nematode (Heterodera avenae)
...was made to optimize the fermentation and mass production of Pseuodomonas spp. Among all the media compositions, the suitable media containing carbon and nitrogen sources were found as media No.9 (Corn flour 50g/L, Glucose 8g/L, Soybean Cake Powder 20g/L, Wheat bran 20g/L, NaCl 4g/L) followed by No.10 (Corn flour 40g/L, Soybean Cake Powder 30g/L, Yeast Extract 5g/L, Wheat bran 20g/L, NaCl 4g/L, KH2PO4 5/L, MgSO4 1.5g/L), No.08 (Corn flour 17g/L, Glucose 10g/L,...
Muhammad Irfan*, Saeed Gul*, Mohammad Younas*, Babar Nawaz*, M. Ishfaq*, M. Amir Durrani*, Abid khan*
FERMENTATION OF SUGARCANE MOLASSES BY SACCHARAMYCES CEREVESIA: EFFECTS OF OPERATING PARAMETERS ON ETHANOL PRODUCTION
...erted to ethanol through fermentation process. A pilot plant has been designed and fabricated to investigate the effect ofprocess parameters for the sustainable production of ethanol from sugar cane molasses. The effect of various experimental paramete...
Tehmina Aslam, Shagufta Saeed*, Muhammed Tayyab, Huma Mujahid, Ali Raza Awan, Sehrish Firyal and AbuSaeed Hashmi
...d of PHB was obtained by fermentation of 4% (w/v) wheat bran after 48 h of incubation with 4% (v/v) inoculum volume at pH 7.0 and 37oC. Among different nitrogen sources tested, 0.2% peptone gave the better yield (285 mg/100 mL), while yeast extract decreased the amount of PHB. The outcomes of the present data indicate that agricultural wastes can be used for the production of polyhydroxybutyrate that will help in the solid waste management.
Tehreem Zahra1, Muhammad Irfan1, Muhammad Nadeem2, Misbah Ghazanfar1, Qurratulain Ahmad3, Shaukat Ali4, Farzana Siddique5, Zarina Yasmeen6, Marcelo Franco7
...s substrate in submerged fermentation by response surface methodology has been investigated. Three variables like substrate concentration, peptone and KH2PO4 were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity was obtained after 96 hours of incubation with optimum conditions of media containing 5% substrate concentration, 0.05% peptone and KH2PO4&...
Ye Ge1, Chunmiao Zhao1, Weitian Xie2, Ying Liu2,* and Chunhou Xu1,*
.... After 7 generations of fermentation, the average pH value was 4.47. The average number of colonies in the culture medium was 18.4. The production of protoplasts lays a foundation for research in microecological preparation.
...
Imrana Khushk1, Abdul Nabi Jatt2, Abdul Sattar Qureshi1*, Choudhary Haider Ali3, Muhammad Aqeel Bhutto1, Imran Ibrahim Khaskheli1, Sartar Iqbal Panhwer1
...ous saccharification and fermentation (SSF). However, onsite produced cellulase does not need enzyme purification, packing and transportation. Hence, the production of bioethanol and lactic acid cost reduced at large extent by using on-site produced cellulase in the saccharification of lignocellulosic material.
Novelty Statement | In house cellulase production saved enzyme production, purificati...
Jia Li and Hong Yang*
...d sludge (WAS) anaerobic fermentation. During the experimental study, the biodegradation efficiency of BaA was enhanced from 12.2% in the control to 25.6% at pH 10 and 46.7% at pH 10 and alkyl polyglucosides (APG) reactors. APG and alkaline treatment increased the BaA deposition desorption from the sludge and transfer into micro-organisms and finally increased the BaA bioavailability. In the meantime, the enormous secretion of substrates from the WAS not only ...
Sadarman1, Agung Irawan2, Muhammad Ridla3, Anuraga Jayanegara3*, Nahrowi3, Roni Ridwan4, Ahmad Sofyan5, Hendra Herdian5, I Nyoman Guna Darma5, Teguh Wahyono6, Dewi Febrina1, Rakhmad Perkasa Harahap7, Rizki Amalia Nurfitriani8, Danung Nur Adli9
... the SSR with or without fermentation. In conclusion, extracted CT and HT from acacia and chestnut showed a protective effect on the potentially degradable nutrients from soy sauce residue and their effects were similar either on ensiled SSR or non-ensiled SSR.
Keywords | Acacia, Chestnut, Nutrient degradation, Soy sauce residue, Tannins
...
Dewi Ratih Ayu Daning1,2, L.M. Yusiati1, C. Hanim1, B.P. Widyobroto1*
...f rumen microbes and its fermentation parameters in vitro. It applies five treatments with six replications and uses completely randomized design to analyze the data. Only if do such differences exist, the Duncan Multiple Range Tests (DMRT) were conducted. The treatments consist of the following galangal EO doses: 0, 30, 60, 120 µL, and 5 µL pure cineole at 300 mg dry matter feed. The experiment proves that gas production, methane (CH4), dry matter...
Seon-Ho Kim1, Mahfuzul Islam1,2, A-Rang Son1, Sang-Suk Lee1*
...breed of steers on rumen fermentation and bacterial community composition in Korea during the summer seasons. Three Holstein and 3 Jersey steers were fed a high forage (HF) and a high concentrate (HC) diet for two subsequent periods of 21 days under 2 × 2 factorial arrangements. The steers received HF diet had higher pH (P < 0.05). Higher acetate concentration was recorded in the Jersey steers and those received HF diet; however, the Holstein steers a...
Jarmuji Jarmuji1,3, Lili Warly2*, Mardiati Zain2, Khasrad Khasrad2
...feed on optimizing rumen fermentation products, digestibility of nutrients, and gases production. Sakura block plus is a modification of commercial sakura block by using earthworm flour as a source of protein as a substitute for rice bran. The treatments were arranged as follow: P0= OPF + concentrate + 10% commercial sakura block, P1 = OPF + concentrate + 6% sakura block plus, P2 = OPF + concentrate + 8% sakura block plus, P3 = OPF+ concentrate + 10 % sakura b...
Hung Van Mai1, Tuan Duc Nguyen2, Nang Thu Tran Thi3 and Hung Phuc Nguyen4*
...he FSBM1 was produced by fermentation of defatted soybean meal (SBM) with Bacillus subtilis B3. The FSBM2 and FSBM3 were commercially available products, which were produced by fermentation of SBM with Lactobacillus spp. Five diets were formulated and denoted as FMD (fish meal-based diet), SBMD (SBM-based diet), FSBM1D (FSBM1-based diet), FSBM2D (FSBM2-based diet) and FSBM3D (FSBM3-based diet). Each diet was fed twice daily ...
Soliman Mohammed Soliman
...rom ruminants during the fermentation of feed represents a loss of energy and contribution of greenhouse gas. Feed additives are one of the various dietary strategies that have been used to mitigate and reduce methane production in dairy cows. This aimed to determine the effects of three varieties of feed additives (green seaweed, probiotics Saccharomyces cerevisiae or Yucca schidigera) on in-vitro total gas and methane production, in-v...
Modification of In Vitro Methanogenesis and Rumen Fermentation by using Lauric Acid: A Meta Analysis
Ayu Cusiayuni1, Risan Khaerani Nurfatahillah1, Rakhmad Perkasa Harahap2, Komang Gede Wiryawan3, Dwierra Evvyernie3, Anuraga Jayanegara3*
... acid-rich oil, on rumen fermentation and methanogenesis by using a meta-analysis approach from published articles. A total of 89 data points from 24 articles were compiled in a database, consisted of various information, i.e., level of lauric acid, microbial population, rumen fermentation characteristics, total gas production, methane production, and digestibility. The data obtained were analyzed by using the mixed-model me...
Nuraini Nuraini*, Mirzah Mirzah, Yuliaty Shafan Nur, Harnentis Harnentis
...s high fiber. Therefore, fermentation with lignocellulolytic fungi was carried out to improve the nutritional quality of Azolla microphylla. This research has 2 phases. Phase 1, determination of the best types of lignocellulolytic fungi on the nutrient quality of fermented Azolla microphylla. This study used an experimental method with a Completely Randomized Design (CRD). The treatments were the types of fungi, namely Lentinus edodes, Pleurotus ostreatus, and...
Yunilas Yunilas1*, Nurzainah Ginting1, Tri Hesti Wahyuni1, Muhammad Zahoor2, Nelzi Fati3 and
Ahmad Wahyudi4
...microorganisms (MOL) and fermentation time on the physical quality of corn straw silage. This study used a completely randomized design, factorial 3 × 3 with three replications. Factor I was various doses of MOL (1 %, 3 %, 5 %), and Factor II was different fermentation times (7 d, 14 d, 21 d). The variables observed included texture, color, odor, and taste. The results showed that the MOL dose and
De Hu, Jia Wang, Huan Wang, Xiang-Yang Leng, Tian-Yi Zhao* and Shu-Min Wang*
...tential mechanism of the fermentation product (FP) of Calvatia lilacina on gastric ulcer, fifty mice were randomly divided into 5 groups (10 mice per group), namely control (normal saline), model (normal saline), ranitidine group (0.03 g/kg) and FP groups (1 and 2 g/kg). Stress gastric ulcer was induced by water-immersion and restraint stress (WIRS) after 10 days of drug administration. To observe the histopathological changes of gastric mucosa. the activities...
Wafaa M.A. Ghoneem*, Muhammad M. Shaklouf, Ali M. Ali and Mohammed H. Bakr
...actobacillus acidophilus fermentation product (LAFP) commercially named (Culbac®) on nutrients digestibility, milk yield and composition, blood biochemistry and immune system response of the animals. Culbac® was added at 0, 10 and 20g/h/d in G1 (control), G2 and G3, respectively. Digestion coefficients of the most nutrients and nutritive value did not significantly (P<0.05) differ between G2 and G1. However, G3 recorded lower values. WBCs, RBCs, Ht,...
Shorouk Aladdin Helmy1, Hossam Mahrous Ebeid2*, Mohamed Ahmed Hanafy1, Adel Eid Mohamed Mahmoud1, Reham Roshdy Ali El-tanany1
...sions and modulate rumen fermentation. An in vitro experiment was conducted to determine the effect of adding three different clay minerals in four levels of each, as follows: humic acid (0.0, 0.5, 1.0, and 1.5%); zeolite (0.0, 1.25, 2.5, and 5%); or bentonite (0.0, 1.0, 2.0, and 3.0 %) with three different protein sources, (soybeans, sunflowers, or cottonseed meals) on a dry matter basis. All rations were incubated in a rumen culture medium collected from she...
Md. Enayet Kabir1, Md. Jahangir Alam1*, Md. Mosharaf Hossain2, Zannatul Ferdaushi3
...h or after its probiotic fermentation along with concentrate mixture in the form of TMR produced a better response in fattening cattle than the conventional system of feeding concentrate and straw separately.
Keywords: Total mixed ration, Blood profile, Fattening cattle, Performance, Faecal bacteria, Probiotic
...
The Effect of Pomegranate Peel on Soybean Meal Protein Degradation and Milk Production in Dairy Cows
Soliman Mohammed Soliman1*, Mohsen Mahmoud Shoukry2, Ahmed Mohammed El-Okazy1, Ahmed Mahmoud El-Morsy1, Mahmoud Mohammed Soliman1
...duction, in vivo ruminal fermentation and milk production using eighteen lactating crossbred Friesian cows that were randomly assigned to three groups. The first group contains untreated SBM, while the second and third groups contain SBM treated with pomegranate peel (PP) at levels of 200 and 250 gm / kg of SBM, respectively. All levels of PP treated to SBM in the first experiment had a positive effect on SBM ruminal degradability after 72 h of incubation. But...
Mimi Syazwani Jaapar1, Eric Lim Teik Chung1,2*, Nazri Nayan1, Mamat Hamidi Kamalludin1,2, Mookiah Saminathan3, Kalai Vaani Muniandy2, Muhammad Hazziq Mohd Hamdan1, Shokri Jusoh1, Faez Firdaus Abdullah Jesse2,4
...s production and ruminal fermentation characteristics. Graded levels of B. decumbens were mixed with P. purpureum, where 10% was identified as the low-level B. decumbens diet (T2) and, 60% was identified as the high-level B. decumbens diet (T3) based on the concentration of saponins. Meanwhile, 100% P. purpureum was used as the basal diet, which served as control (T1). Rumen fluid was then collected from six young male Dorper cross sheep for the analyses of ga...
Umar Farooq Gohar, Attia Majeed, Bushra Muneer* and Hamid Mukhtar
...s and parameters such as fermentation medium, pH, temperature, inoculum size, carbon and nitrogen sources were optimized to attain maximum production of podophyllotoxin. The maximum production of 566.23µg/ml was achieved with yeast extract sucrose broth medium with 15% and 5% supplementation of glucose and peptone as carbon and nitrogen source, respectively at pH 5.5 and temperature 25ºC. The endophytic fungi emerging as an alternative source of suc...
Desy Cahya Widianingrum1*, Himmatul Khasanah1, Melinda Erdya Krismaputri1, Susan Barbara Patricia Sembiring Meliala2
...ty, rancidity, and rumen fermentation characteristics and in vitro digestibility. The data obtained were analyzed using Analysis of Variance (ANOVA) and further tested with an Honestly Significant Difference (Hsd). The highest significant difference in physical quality of wafer (P<0.05) were found among treatments. The P4 group had the highest density (0.230 ± 0.16 g/cm3), while the P1 group had the highest absorption (77.60%). The P0 group had the h...
Haitham Al Masruri1, Rashid Al Zeidi2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Ahmed Al-Madani4, Al-Hassan Mohammed5*
...ent digestibility, rumen fermentation and enzyme activities, growth and reproductive performances, antioxidants, and milk production and composition have been well documented. M. oleifera contains valuable chemical constituents such as proteins, essential fatty acids, vitamins, and beta-carotene. The M. oleifera composition varies depending on nutrient availability for production, species, and lifespan of trees. Extracts of leaves and seeds were used for the p...
Razaq Adekunle Animashahun1*, Gbenga Emmanuel Onibi2, Samuel Olanrewaju Aro2, Oghenerobor Benjamin Akpor3, Funmilayo Abimbola Okeniyi1, Olayinka Olubunmi Alabi1, Michael Babatunde Falana1, Ayoola John Shoyombo1, Samuel Oyewale Olawoye1
... results showed that the fermentation media and the fermentation period significantly (P < 0.05) affected the nutritional and anti-nutritional components of the cassava stumps, as there was better enhancement of the by-product at higher fermentation period. The highest crude protein (CP), ether extract (EE), ash, and lowest crude fiber (CF) in fermented cassava stumps were obtained at 1...
Hurriya Alzahra1, Susmiati Susmiati2*, Sri Melia3
...al food derived from the fermentation of milk with probiotic lactic acid bacteria, specifically the Lactiplantibacillus pentosus HBUAS53657. This study aims to determine the antioxidant activity, total phenol, and organoleptic characteristics including the taste, aroma, and texture of fermented milk with orange juice added (Citrus nobilis L.). The study was based on a Factorial Completely Randomized Design (CRD), with starter concentrations of 4 (A1), 5 (A2), ...
Nuraini Nuraini*, Yuliaty Shafan Nur, Ade Djulardi, Robi Amizar, Yesi Chwenta Sari
...p>
Keywords | Medium fermentation, Natura, Tenebrio molitor larvae, Nutrient content, Broiler performance
...
Pham Tan Nha*, Le Thu Thuy
...) with 2 factors. Factor fermentation: Yeast-fermented maize (YM), Yeast-fermented broken rice (YR), Probiotic fermented maize (PM). Factor protein level: 20% (CP20) and 16% (CP16). One hundred and eighty Noi chickens at 6 weeks of age (334±9.5 g/bird), and 10 birds per unit (balanced sex) were in the treatment. All treatments were added 4% (DM) of fermented feed to the basal diet. The trial lasted 9 weeks with Noi chickens from 6 to 14 weeks of age. Th...
Ahmed M. El-Waziry1,2*, Saeid M. Basmaeil1, Ibrahim A. Alhidary1, Gamaleldin M. Suliman1,3, Mutassim M. Abdelrahman1, Maged A. Al-Garadi1
...of ionophores on ruminal fermentation, performance and characteristics of carcass of goats.
Keywords | Ionophores, Ruminal Fermentation, Animal Performance, Carcass Characteristics, Meat Quality
...
Optimization of Process Parameters for the Enhanced Biosynthesis of ⍺-Amylase by Bacillus subtilis
Uzma Hameed1*, Aneela Liaqat1, Muhammad Asjad Khan2, Ikram-Ul-Haq1 and Maham Aslam1
...timizing the solid state fermentation (SSF) parameters. In this context, the fermentation rate for optimal alpha-amylase production was optimized using a solid substrate, wheat bran. Optimal enzyme production (2.1±0.15 U/ml) was achieved with 48 hours fermentation period. A considerable increase in enzyme activity (4.5±0.031 U/ml) was observed when diluent with initial pH of ...
Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2
...ugarcane bagasse using a fermentation process. Amylase was partially purified from the bacterial strain N1 and its activity was checked at distinct pH and temperature. The optimum pH was from 6-8 whereas the temperature was 40 °C. Saccharomyces cerevisiea was added for enhanced fermentation and ethanol production. The acid pretreated sample produced the highest ethanol (15.2%) than the alkaline. The percent production of...
Amir Abdullah Khan and Muhammad Irfan*
...cultivated in sub-merged fermentation for the production of bioactive compounds. Various parameters were optimized to obtain maximum bioactive compounds production. Maximum antifungal activity was observed at temperature 37°C, pH 8, inoculum size 2.5% and 24h fermentation period. These results recommended the potential utilization of Bacillus subtilis 2i for intention to control the disease causing fungus Schizophyllum s...
Sepri Reski*, Ridho Kurniawan Rusli, Montesqrit, Maria Endo Mahata
...binaria murayana seaweed fermentation products with different levels showed a very significant effect (P≤ 0.01) on the cholesterol content of egg yolks. However, no significant effect (P≥0.05) on egg weight, shell thickness, shell percentage, yolk percentage, white percentage and egg fat content. This study concludes that Turbinaria murayana seaweed fermentation products can be used in laying quail rations up to 20% by...
Mohamed S. Abbas1*, Adel E.M. Mahmoud2, Hemat S. Mohamed3, Adam Cieślak4 and Małgorzata Szumacher-Strabel4
...concentration on ruminal fermentation, pH, ammonia (NH3), total gas production (TGP), methane and volatile fatty acids (VFA) concentration. Twenty-six wild palatable forage plants were identified and analyzed for pH, NH3, TGP, methane and VFA. The results indicated that in grasses the highest pH and NH3 concentration was found in Aeluropus lagopoides, TGP in Ammophila arenaria and methane in Phragmites australis. In legumes the highest concentration of pH was ...
Hafiz Abdullah Shakir1*, Iqra Javed1, Muhammad Irfan2*, Shaukat Ali3, Muhammad Khan1, Farah Rauf Shakoori1, Javed Iqbal Qazi1 and Muhammad Abrar Yousaf1
...inolytica in solid state fermentation (SSF) using corn (Zea mays) leaves as substrate to reduce its production cost. The maximum tannase production was obtained with 60% initial substrate moisture contents, tap water as enzyme extraction medium with 2 mL volume, 45°C incubation temperature, pH 7, 300 µL inoculum size, 24 h incubation period in agitated condition with substrate particle size of 4mm during one factor at a time optimization. Concentrati...
ANSA KHALID, UZMA HAMEED* AND IKRAM-UL-HAQ
.... Among the 10 different fermentation media screened for enzyme production, maximum dextransucrase activity (9.96 U/ml) was obtained by bacterial strain IIB-C9 in fermentation medium M6 comprising (yeast extract 15 g/L, sucrose 40 g/L, and Na2HPO
ANSA KHALID, UZMA HAMEED* AND IKRAM-UL-HAQ
.... Among the 10 different fermentation media screened for enzyme production, maximum dextransucrase activity (9.96 U/ml) was obtained by bacterial strain IIB-C9 in fermentation medium M6 comprising (yeast extract 15 g/L, sucrose 40 g/L, and Na2HPO
Ovirup Bhushan Paul1, Shodipta Sharma Urmi2, Md. Ashraf Ali Biswas3*
...mplemented that combined fermentation to improve digestibility with locally accessible feed supplies using a completely randomized design. TMR feed was produced with 70% roughage of silage & rice straw and 30% concentrate mixture. FTMR feed was produced by mixing TMR feed with molasses containing Saccharomyces cerevisiae. Prepared TMR and FTMR feed mixed with rumen fluid buffer for in vitro digestion at 6h, 12h, 24h, & 48h of the incubation period. The...
Theresia Nur Indah Koni*, Tri Anggarini Yuniwati Foenay, Stormy Vertygo
...ber is through anaerobic fermentation, which requires carbon from soluble carbohydrates such as palmyra sap and nitrogen from Non-Protein Nitrogen such as urea. This experiment aimed to evaluate the fermentation characteristics and nutrient content of rice bran fermented anaerobically for six days. This experiment used a Completely Randomized Design with four treatments and four replications. The treatments included rice bra...
Munasik*, Titin Widiyastuti, Caribu Hadi Prayitno
...titutes for corn through fermentation technology using Neurospora sp. This produces alternative feed ingredients with nutrient levels that are in balance with corn. To improve the quality of the cassava-tofu dreg mixture, this study used red oncom mushrooms enriched with Na-glutamate during the fermentation process. The research used a completely randomized factorial design, where two factors were considered: the addition le...
Hamdi Mayulu1*, Endang Sawitri2
...btained from the thermal fermentation process which comprises incubation at controlled temperature and humidity for several weeks. During this process, browning and non-enzymatic reactions alter the taste, aroma, physicochemical properties, and organoleptic and bioactive compounds in garlic. Therefore, this study aims to investigate the phytochemical potential and antioxidant capacity of black garlic as a livestock feed additive. The Folin-Ciocalteau method wa...
Muhammad Adeel Farooq1, Shaukat Ali1*, Ali Hassan1, Rida Sulayman1, Muhammad Ahsan Kaleem2, Hafsa Shahzad1, Muhammad Summer1, Arooj Latif1, Tahreem Tanveer1
...a;-amylase via submerged fermentation. Then the crude α-amylase synthesized by these mutant strains was optimized and partially characterized. In contrast to wild and all other mutant strain BSAA-25 and BLAA-25 strains showed the optimum production of α-amylase 331.4±6.9 U/mL and 310.8±11.3 U/mL, respectively, at 37±0.5°C and pH 7.0±0.2 for 48 h on wheat bran-based broth. BSAA-25 demonstrated maximum biosynthesis of ...
Farha Deba1, Rubina Nelofer2 and Muhammad Irfan1*
... evaluated via submerged fermentation utilizing raw feathers as a substrate for keratinase production. On skimmed milk agar plates with streaked pure culture growth patterns, these seven strains gave a positive appearance of sizable dramatic zones around the growth patterns. These seven strains such as Bacillus velezensis FD1, Bacillus cereus FD2, Bacillus subtilis FD3, Bacillus altitudinis FD4, Bacillus licheniformis FD5, Bacillus flexus FD6 and Alcaligenes s...
Amirul Faiz Mohd Azmi1,4, Hafandi Ahmad1, Norhariani Mohd Nor1, Goh Yong Meng1, Mohd Zamri Saad2, Md Zuki Abu Bakar1, Norafizah Abdul Rahman5,6, Agung Irawan7,9, Anuraga Jayanegara8,9, Hasliza Abu Hassim1,3,9*
...nt on the in-vitro rumen fermentation and microbial ecosystem of Murrah cross and Swamp buffaloes. Three males Murrah cross and Swamp buffaloes consuming 100% DM of fresh B. decumbens were used as rumen contents donors. The in-vitro ruminal fermentation and microbial population profiles were investigated. The study revealed that Diet C had the highest ether extract and gross energy, with optimum value of crude proteins but l...
Yao Xixi1, Zhou Rui2* and Ma Yinshan3
...rient composition, rumen fermentation parameters and content of conjugated linoleic acid (CLA) in milk of grazing yak. In this study, 10 female Gannan yaks with an average body weight of 234.9±10.5 kg were selected for research purpose (natural grazing) in Gannan Pastoral Area of Gansu Province. The forage, rumen fluid and milk samples were collected during green-up time, flowering time and withering time of forage to determine nutrient composition of f...
Wizna1, Rusfidra2, Robi Amizar3, Romi Andika1, Muhammad Haikal1, Zurmiati1*
...dy aims to the effect of fermentation of leftover food from restaurants and hotels with Bacillus amyloliquefaciens on total colony count of Bacillus sp. and nutrition content of leftover food. The research, a 3x3 complete randomized design factorial, consisted of two factors, and each treatment was repeated three times. Factor A is the dose of B. amyloliquefaciens (3, 4, and 5%), and factor B is the fermentation ...
Hung Phuc Nguyen1*, Doan Van Thuoc2*, Nguyen Thi Trung Thu1, Huong Tran Thi Mai3, Nguyen Tran Khanh Hoa1, Nguyen Thi Tuyet Nhi1, Nguyen Phuong Thao1, Tran Thi Loan2 and Nguyen Thi Huyen My2
...ded diets suggested that fermentation of SR with Bacillus subtilis V37 benefited digestive physiology, growth performance and feed utilization of red tilapia fed soybean protein-based diets....
Sherein H. Mohamed1, Soad El Naggar2*, Ayman A. Hassan3, Mohamed A.M. Mousa3, Mohamed M. Basyony3, Mohamed F. Sadek3, Mohamed A. A. Ahmed4, Saadia M. Hashem5
...ce, digestibility, cecal fermentation, antioxidant enzyme activities, immunity and meat quality. One hundred twenty six male growing New Zealand White rabbits aged eight weeks old with an average body weight of 675±47.95 g were divided into six equal groups 21 rabbits each, control diet group, C fed the basal diet and the other five groups (GE, ClE, ThE, TE, and CE) fed on basal diet supplemented with 200 ppm of garlic, clove, thyme, turmeric and cinnam...
Yunilas1*, Muheri Indra Aja Nasution2, Edhy Mirwandhono1, Adi Fathul Qohar3
... chitin content first by fermentation using organic acids. Therefore, this study aimed to determine the effect of using organic acids (propionic acid and formic acid) and various fermentation times in reducing the BSFP chitin content. Completely Randomized Design (CRD), factorial 3 x 3 with three replications. Factor I was various doses of organic acid (3%, 6%, 9%), and Factor II was different fermen...
Nurul Isnaini*, Dedes Amertaningtyas, Hanief Eko Sulistyo, Aulia Puspita Anugra Yekti, Faizal Andri
Zain Ali1, Amjed Ali1, Bilal Ahmad Khan1*, Muhammad Ather Nadeem1, Muhammad Asif1, Adnan Ashraf1, Muhammad Ehsan Safdar1, Iram Inayat2, Aneela Nijabat3 and Rameez Hussain3,4
...ure content preserved by fermentation. The main objectives of making silage are to preserve forage for use in those seasons when fresh forage is not available, to preserve forage when there is a surplus of it during the growing season, and to preserve forage that cannot be grazed. The experiment was carried out at research area, Department of Agronomy, College of Agriculture, University of Sargodha to evaluate the combined effect of sunflower and maize crop fo...
Nguyen Huu Van1*, Nguyen Thi Mui1, Dinh Van Dung1, Van Ngoc Phong1, Tran Ngoc Long1, Le Tran Hoan1, Le Duc Thao1, Vo Thi Minh Tam1, Ngo Mau Dung1, Bui Van Loi1, Nguyen Xuan Ba1, Ton Nu Minh Thi2, Nishino Naoki2
... digestibility and rumen fermentation of the animals.
Keywords | Phan Rang sheep, Concentrate, Rumen fermentation, Digestibility, Growth, Carcass
...
Nahariah Nahariah1*, Hikmah Hikmah1, Faridah N. Yuliati1, Endah Murpiningrum1, Abdul W. Jamaluddin2, Titis S. Kusuma3, Andi Febyana4, Kartina Kartina1
...our is influenced by the fermentation process. We aimed to identify the application of yeast Saccharomyces cerevisiae levels with different fermentation times in egg yolks to the functional and physicochemical characteristics of egg yolks. This research was conducted using a 3 x 3 factorial Completely Randomized Design with 5 replications. Factor A was the fermentation times (hours) 1, 2, ...
Firda Dimawarnita1*, Edwina Gabriela Tesalonika Mustamu2, Yora Faramitha1, Haryo Tejo Prakoso1, Wildan Aulia Noorsy1, Komang Gede Wiryawan2
...ess and in-vitro rumenal fermentation. The treatments were raw EFB (P0) and delignified EFB (P1). We evaluated rumen pH, total volatile fatty acids (VFA), ammonia concentration (NH3), dry matter digestibility (DMD), crude fiber digestibility (CFD), organic matter digestibility (OMD), methane gas production, fulvic acid content, and bacterial population. The results indicated that the treatment did not have a significant effect on DMD, OMD, pH, NH3 concentratio...
Theresia Nur Indah Koni*, Yeria Banoet, Welhelmina Wahon, Cytske Sabuna, Melkianus Dedimus Same Randu, Yanse Yane Rumlaklak, Tri Anggarini Yuniwati Foenay
... the effect of anaerobic fermentation on the nutrient content of banana peels and their use on performance of crossbred native chickens. Banana peels were fermented (FBP) anaerobically for 21 days with addition of 10% tapioca flour as an additive. Then banana peels without and with fermentation were analyzed for their nutrient content. This fermented banana peel was then applicated to the crossbred native chicken diet with d...
Siqiang Li1,2, Tiantian Wang1, Peng Sun1, Airong Gao1, Xin Gong1, Yuanhong Xu2, Baogen Wang2, Jun Wu1* and Bo Liu1*
...d expressed in a 5-liter fermentation tank. The target protein was purified in three-step purification and identified by peptide mass of fingerprint. The enzymatic activity and optimal reaction conditions of MDS I were detected using DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. We obtained MDS I with a purity exceeding 90% in gram scales, which was capable of digesting α-1,2 linked mannose residues in high selectivity. The hi...
MARIAM AHMED, YESRA ARSHAD & HAMID MUKHTAR*
...s substrate in submerged fermentation. Two isolates, showing maximum enzyme production, were identified as Bacillus species on their morphological and biochemical basis. Crude enzyme revealed that Bacillus sp. IIB-B5 produced maximum keratinase (28.13 U/mL) at pH 7.0, at 40oC after 72 h incubation. While Bacillus sp. IIB-B9 showed maximum enzyme production (26.59 U/mL) at pH 8.0, after an incubation period of 96 h at 50oC. This study showed that these isolates...
MARIAM ZAHEER*1, SYED SHAHID IMRAN BUKHARI2, MUDDA
...ibes sources, synthesis, fermentation, extraction and applications of exopolysaccharides....
Putri Okta Shafura1, Mardiati Zain2*, Elihasridas2, Bima Bagaskara1, Ummi Amanah1, Laras Sukma Sucitra1, Bella Veliana Utami1, Roni Pazla2, Erpomen2, Ezi Masdia Putri3, Rusmana Wijaya Setia Ningrat2, Riris Delima Purba3, Ruslan Abdul Gopar3, Putut Suryo Negoro3
... digestibility and rumen fermentation characteristics. The combination of 0.5% S. cerevisiae and 0.3% sulfur tended to increase nutrient digestibility, total VFA production, and microbial protein synthesis. Additionally, there was a notable difference (P > 0.05) in rumen pH. Thus, the ammoniated citronella ration supplemented with 0.5% S. cerevisiae + 0.3% sulfur tended to decrease NH3 production, protozoa population, and methane gas production. The results...
Evy Rossi1*, Fajar Restuhadi1, Raswen Efendi1, Yusmarini1, Rahmayuni1, Emma Riftyan1, Bisma Panca Winata2, Yolanda Ashara2, Usman Pato1
...plied using back-sloping fermentation for the practicality of the yoghurt-making process. This research aimed to determine how many cycles the back-sloping fermentation using microencapsulated yogurt (MY) starter was applied and evaluate the quality of yogurt in each back-sloping fermentation cycle. The lactic acid bacteria contained in MY starter were Lactobacillus plantarum TMW 1.1623 an...
Suparada Saphaphan, K. Teepalak Rangubhet, Phongthorn Kongmun*
...d rations (TMR) on rumen fermentation and microbial ecology using an in vitro gas production technique. Four treatments, Pangola grass hay (PH), rice straw (RS), MSGBTRS, and 3.5% urea-treated rice straw (UTRS), were mixed thoroughly with the concentrated feed ingredients to make TMR at a ratio of 60:40. Treatments were assigned in a completely randomized design. Results showed that cumulative gas production at 72 h was significantly higher (P<0.05) in the ...
Pazir Gul and Fazli Wahid Khan
...reparation of alcohol by fermentation....
Budi Santoso*, Bambang Tjahyono Hariadi, Marlyn Nelce Lekitoo
...he chemical composition, fermentation properties, nutritional digestibility, and CH4 generation of oil palm frond-based complete feed silage to assess the impact of a cellulase additive. In the complete randomized design experiment, six treatments (silage) were used: A, B, C, D, E, and F. Treatment A consisted of oil palm frond (40%), king grass (30%), cassava (10%), tofu waste (10%), molasses (7%), lactic acid bacteria (LAB) inoculant (3%), and cellulase 0 ml...
Duong Thanh Hai1*, Phan Thi Hang1, Nguyen Thi Thuong2, Zábranský Luboš3
... whether effects of feed fermentation on carcass characteristics, meat quality and amino acid contents remain unknown. This study was conducted to examine the effects of maize and rice bran fermented with Saccharomyces cerevisiae on carcass characteristics, meat quality and amino acid composition of (Ri x Luong Phuong) chicken. The study was carried out using two hundred forty crossbred chickens begging at 28 days of age. Chickens were arranged randomly into t...
Mati Ullah1*, Muhammad Rizwan2, Ali Raza3, Xianlin Zhao4, Yanling Sun4, Sarah Gul5, Muhammad Ihtesham Waheed6, Muhammad Nadeem Khan7, Alvina Gul8, Sami Ullah Jan9* and Chao Huang4*
...lly, it could be used in fermentation for selective purposes. In the current study, we have comprehensively analyzed the genome of Lactobacillus delbrueckii subsp. bulgaricus NDO2 isolated from traditional yogurt samples using the next-generation sequencing approach. The GC contents of the selected genome were 45.3%. Among the total 2284 genes identified, 970 genes similar in three genomes make the core genome portion. The subsystems, category distribution ana...
Muhammad Ardas Daruslam1, Muhammad Irfan Said2*, Nancy Lahay3
...a gas reducer during the fermentation process, namely: P0 = without Ec-En (control); P1 = 100% of Ec-En + 0% water (v/v), P2 = 90% of Ec-En + 10% water (v/v) and P3 = 80% of Ec-En + 20% water (v/v). Fermentation time uses three levels, namely: T0 = 0 minutes; T1 = 15 minutes and T2 = 30 minutes. Fermentation time is the time after Ec-En is applied to the feces of laying hens. Data we...
Usman Ali*, Badat Muwakhid and Dewi Masyithoh
Syeda Rubab Zaidi* and Safdar Ali Mirza
...us niger under submerged fermentation technique. Optimization of different cultural parameters that influence laccase enzyme biosynthesis by A.niger strain was done. To measure enzyme activity indicator compound guaiacol and acetate buffer were used. It has been found that laccase activity was maximum on day 9 of incubation when operated under following cultural conditions, 2 pH, 35ºC, 3.0 % wheat straw as carbon source among other synthetic (glucose and ...
Nurhayati1*, Chandra Utami Wirawati2, Dwi Desmiyeni Putri1
...addition of minerals and fermentation duration. The fermentation experiment was designed using a Completely Randomized Design with a 4x6 factorial pattern with three replications for each treatment combination. The first factor was the level of mineral addition (M0 = no mineral addition, M1 = mineral addition with the composition: 2.5 g KCl, 12.5 g ZA, 7.5 g urea, and 2.5 g NPK, M2 = mineral addition with the composition: 5 ...
Nurhayati1*, Chandra Utami Wirawati2, Dwi Desmiyeni Putri1
...addition of minerals and fermentation duration. The fermentation experiment was designed using a Completely Randomized Design with a 4x6 factorial pattern with three replications for each treatment combination. The first factor was the level of mineral addition (M0 = no mineral addition, M1 = mineral addition with the composition: 2.5 g KCl, 12.5 g ZA, 7.5 g urea, and 2.5 g NPK, M2 = mineral addition with the composition: 5 ...
Muhammad Ambar Islahuddin1, Yuli Retnani2*, Despal2
... well as their impact on fermentation and digestibility in dairy cows in vitro. Molasses with viscosities of 320 mPas-1 and 6400 mPas-1 were utilized alongside pollard binders, cassava meal, and cassava waste. The experimental design employed a 2x3 factorial pattern CRD with 5 replicates. Analysis of Variance and DMRT were employed to assess treatment differences, focusing on moisture content, water activity, specific gravity, and wafer durability index. The b...
Nadlirotun Luthfi1, Edy Rianto2*, Endang Purbowati2, Christina Maria Sri Lestari2, Agung Purnomoadi2, Nurul Mukminah3
... was to evaluate ruminal fermentation, methane emissions, and nitrogen excretion of young and mature Kacang goats at different feeding levels. Sixteen goats were divided into two age groups (young and mature) and fed at either low feeding level (maintenance) or high feeding level (production). The ration contained 18% crude protein (CP) and 75% total digestible nutrients (TDN). Parameters evaluated included nutrient intake, acetate, propionate, and butyrate co...
Muhammad Ariana Setiawan1, Ujang Hidayat Tanuwiria2*, Andi Mushawwir2
...in facilitating the feed fermentation process. Therefore, its nutrient requirements must also be taken into consideration. Nutrient requirements for rumen microbes can be met from rumen degradable protein (RDP) and non-fiber carbohydrate (NFC). Nutrients that are easily degraded in the rumen can be seen from their metabolite products such as total gas production, gas kinetics, and methane gas production. This study aims to determine the effect of the balance o...
Bilal Ahmed1, Faheem Ahmed Khan2, Nuruliarizki Shinta Pandupuspitasari1*, Muhammad Rizwan Yousaf1 and Asep Setiaji1
...sulted in use of natural fermentation agents like Saccharomyces cerevisiae that can be methanogenic. The use of Saccharomyces cerevisiae has been under question for it’s possible methanogenic effects. This meta-analysis showed Saccharomyces cerevisiae supplementation did not significantly alter methane emissions (CH4), dry matter intake (DMI), or rumen pH in dairy cows, this study provides valuable insights into future dose-dependent studies to address t...
Saitul Fakhri1*, Heni Suryani2, Teja Kaswari1, Munawwaroh Pane1, Neneng Hariyati1, Dodi Rolis Limbong1, Anuraga Jayanegara3, Daniel Komwihangilo4
Sabahat Anwar1, Faria Javaid2 and Qurat-ul-Ain2*
Dyana Louis Anak Peter, Elldiwirna Saimen, Lucky Poh Wah Goh, Mohd. Khalizan Sabullah, Rahmath Abdulla, Jualang Azlan Gansau and Roslina Jawan*
...cell count until 48 h of fermentation. The viable cells count in the glucose-free MRS medium supplemented with nonistarted at 8.83 × 106 CFU/mL (before fermentation) and increased to 8.67 × 108 CFU/mL (24 h) and slightly decreased to 2.55 × 108 CFU/mL (48 h). Result also demonstrated that the viable cells count in a medium supplemented with bambangan, noniand mung bean signi...
Ali Bain*, Musriah, La Ode Nafiu, La Ode Muh. Munadi, La Ode Muhsafaat, Nur Santy Asminaya, Astriana Napirah, Widhi Kurniawan, Fuji Astuty Auza, Deki Zulkarnain
...dy aimed to evaluate the fermentation characteristics and in vitro nutrient digestibility of feed based on the fermented corn cob supplemented with soybean oil calcium soap (CaS-soybean oil) using microbial rumen fluid of Etawa crossbreed goats. The experimental trial consisted of five treatment rations with four replicates arranged in a randomized block design. The treatment rations were a mix of 60% concentrate (25% fermented corn cobs and 75% agricultural b...
Nesreen Z. Eleiwa1, Radwa A. Lela2, Eman K. Fathalla2
...duct that causes sausage fermentation and enhances its flavor, quality, and shelf life. Therefore, this study was conducted to compare Lactobacillus plantarum, which acts as a control group, and different concentrations of Kefir 1%, 3%, and 5% in the fermentation process of oriental sausages. The results demonstrated that including water Kefir notably improved the sausages’ quality. Specifically, water Kefir 3% and 5% ...
Nuttanun Leamkrajang, Somkiert Prasanpanich, K. Teepalak Rangubhet, Phongthorn Kongmun*
...ically focusing on rumen fermentation characteristics and the population of rumen microorganisms. The investigation utilized a 3×2 factorial in CRD, incorporating four replications. The inoculum sources included three animal species (swamp buffalo, beef cattle, and goat), while the dietary treatments consisted of two roughages to concentrate ratios: 100:0 and 70:30, using dry leucaena leaves as the source of roughage. The kinetic values of
Heni Suryani1, Sri Novianti2*, Fatati2, Jul Andayani2, Saitul Fakhri2, Muhammad Ambar Islahudin3
...nutrient degradation and fermentation in the rumen as indicators. A completely randomized design with 4 treatments and 4 replications was employed, involving binahong extract levels: control, 1%, 1.5%, and 2% based on dry matter (DM). One gram of feed substrate, supplemented with binahong extract, was incubated in buffered rumen fluid at 390C for 48 hours in a 120 ml serum bottle. Gas production was measured at intervals of 2, 4, 6, 8, 10, 12, 16, 20, 24, 36, ...
Maria Endo Mahata*, Yose Rizal, Sepri Reski, Zurmiati, Elsanila Suryadi Buwono, Kamiliya Rahma Sari
...ude fiber was reduced by fermentation using local microorganisms as inoculum. This study aimed to determine the effect of brown seaweed Padina australis, with reduced salt and crude fiber content, on the growth performance and physiological organs of broiler chickens. This study used a Completely Randomized Design (CRD) with brown seaweed Padina australis levels of 0, 5, 10, and 15% in broiler chicken diets, with each treatment repeated five times. The paramet...
Trang Nguyen Phan*, Loc Bao Nguyen, Thi Anh Ngoc Tong
...some features related to fermentation parameters, including pH value, production of total acidity, and CO2. The results revealed that the LAB isolates produced an amount of lactic acid from 6.84 to 28.28 mg/mL with a pH value of 3.38 to 6.19. Most LAB isolates form CO2 from glucose, suggesting that these LAB perform hetero-lactic fermentation. Approximately 78% of LAB exhibited multidrug-resistant strains with various resist...
Featuring
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Primary, Secondary Metabolites and Antioxidant Activity of Castanopsis Argantea, Artocarpus heterophyllus, and Aglaia tomentosa Fruit as Food for the Tapanuli Orangutan (Pongo tapanuliensis) in the Batang Toru Forest, North Sumatra
Herna Febrianty Sianipar, Wahyu Widoretno, Luchman Hakim, Rezi Rahmi Amolia, Fatchiyah Fatchiyah
Adv. Anim. Vet. Sci., Vol. 13, Iss. 1, pp. 64-72
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Development Model of Food Crop-Beef Cattle Livestock Integration Based on Technology and Ecosystem Downstreamization for Improving Social Structure Communities in Gorontalo Province
Mahyudin Humalanggi, Mohammad Zubair Hippy, Muhammad Mukhtar, Sutrisno Hadi Purnomo, Siti Rahmatia Machieu, Nancy Noviana Lantapon, Ivana Butolo
Adv. Anim. Vet. Sci., Vol. 13, Iss. 1, pp. 51-63
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The Effects of Replacing Fish Meal with Skipjack Tuna Offal Meal on the Growth Performance and Carcase Quality OF Local Chickens
Srisukmawati Zainudin, Hartutik, Edhy Sudjarwo, Osfar Sjofjan
Adv. Anim. Vet. Sci., Vol. 13, Iss. 1, pp. 43-50
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Antibiotic Resistance in Lactic Acid Bacteria Isolated from Vietnamese Fermented Foods
Trang Nguyen Phan, Loc Bao Nguyen, Thi Anh Ngoc Tong
Adv. Anim. Vet. Sci., Vol. 13, Iss. 1, pp. 36-42
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