Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2
...tract has relatively low cytotoxicity where the IC50 value of extract against Vero cells was 1078.69 µg/ml. Furthermore, the extract exhibited a considerable anti-HSV-1 and anti-HSV-2 activities at the concentration of 25 μg/ml. In conclusion, our findings indicate that the methanolic extract of Capsicum annuum could be used in combination with standard drug to treat herpes infection....
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
...lls, A549 by determining cytotoxicity of these agents by trypan blue assay, apoptosis inducing ability by annexin V and JC-1 staining and ultrastructural alterations by transmission electron microscopy. Our data clearly shows that ceranib-2 is more effective than C2 ceramide in promoting apoptosis. The cytotoxic effects of these components cause ultrastructural changes in A549 cells.
... 
Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***
ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
... in-ovo and in-vivo. The cytotoxicity assay of the product was performed using Vero cell line. All the trials revealed that the stock solution and 1:2 dilution of GZ-08™ had some antiviral activity as well as were cytotoxic. As the concentration decreased, cytotoxicity as well as antiviral activities were lost. Based on these findings, it was concluded that GZ-08™ sanitizer or ...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
... of time. The micro well cytotoxicity assay against Artemia salina showed the cytotoxicity of the extracts in the range of 10-35%. The chemical profiling of the extracts was done by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC-UV) to get the indication of the identity of the compounds responsible for antimicrobial activity and biofilm dispersion. Overall the results indicated that n...
Kiran Aqil1, Muti-ur-Rehman Khan2, Asim Aslam2, Aqeel Javeed5, Rizwan Qayyum1, Farooq Yousaf,1 Farkhanda Yasmeen1, Muhammad Luqman Sohail3 and Sajid Umar4,*
...ro;g/ml) extracts showed cytotoxicity to Vero cell lines. In the plaque reduction assay, alcoholic extracts of N. sativa (50 µg/ml) significantly reduced the plaques count as compared with the control negative (P<0.05) in all the tested modes of action. The current experiment revealed that the alcoholic extracts of N. sativa (50 µg/ml) could increase the viability of infected cells, and curtail the cytopathic effects of PPRV...
In-vitro Anticancer Activity of Copper (II) and Zinc (II) Complexes with Derived Schiff Base Ligands
Qaisar Jamal1,Salman Ahmad1, Nazma Habib Khan1, Sobia Wahid1, Muhammad Ikram2, Sadia Rehman2, Syed Basit Rasheed1 and Akram Shah1,*
...µM; whereas, their cytotoxicity was 72.26µM, 255µM and 293.8µM, respectively against peripheral blood lymphocytes. Based on the findings of present study the compounds can be put in to list of candidacy for anticancer activity.
...
Rahila Huma1,2, Tariq Mahmud1,*, Rubina Munir2, Sana Javaid Awan3,Kiran Iftikhar4 and Mufakkira-Tul-Islam5
...timated by measuring its cytotoxicity for human breast cancer cell line (MCF-7). Both compounds PFA and PFA-Cd were prepared and subsequently characterized by FTIR, ICP-AES, UV-Vis, TGA, 1H NMR, 13C NMR and FAB-MS. The results were in great consistency with the structures of the both compounds. Furthermore, the ligand and its metal complex were screened for their cytotoxicity against human cancer cell l...
Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*
...en the antimicrobial and cytotoxic activity of Haplophyllum gilesii (Hemsl.) C.C. a narrow endemic plants of northern areas of Pakistan. The antimicrobial potential of H. gilesii extracts in different solvents was assessed using agar well diffusion method against bacterial and fungal strains, while cytotoxic activity was studied in the methanolic extract using brine shrimp’s lethality assay. All the extracts showed sig...
Syeda Farzana Bibi* and Siraj-ud-Din
Fluorescence Analysis, Extractive Values and Cytotoxic Screening of Crataeva adansonii DC Leaf and Bark through Brine Shrimp Larvae Assay
...ardization of drugs. The cytotoxic study helps in the evaluation of a drug for its toxicity, which is determined by the prevention of growth and replication. Such drugs can be used to treat cancer, multiple sclerosis and rheumatoid arthritis. During the present study ethanolic extracts of leaf and bark parts of C. adansonii were evaluated through cytotoxic activity. Brine shrimp larvae assay was conducted for this purpo...
Mahak Fatima1, Memoona Syed1, Rabia Zeeshan2, Farah Rauf Shakoori3, Naveed Shahzad4, Moazzam Ali1 and Zeeshan Mutahir1*
... of siRNA and consequent cytotoxicity in MCF7-R cells was confirmed by qRT-PCR, western blotting and cell viability assay. As a result of siRNA transfection, MTH1 protein was knockdown to approximately 77% in the transfected sample and resulted in a 1.75-fold increase in sensitivity of MCF7-R cells to gemcitabine. Moreover, higher expression of p21 protein was also observed in transfected MCF7-R cells that may indicate induced cell death. This study highlights...
Canan Vejselova Sezer*
...d by using MTT assay for cytotoxicity, annexin-V analyses for cell death mechanism and confocal and transmission electron microscopies for morphological and ultrastructural changes. Based upon our findings, it can be concluded that silymarin-loaded solid lipid nanoparticles significantly reduced the growth of A549 and MCF-7 cells compared to silymarin. Also these nanoparticles induced apoptosis both in A549 and MCF-7 cells in higher percentages than that of si...
Hesham Saeed1*, Manal Abdel-Fattah1, Ahmad Eldoksh1, Farid S. Ataya2 and Manal Shalaby3
Saba Afzal1, Sohail Raza1,*, Masood Rabbani1, Sehrish Firyal2, Imran Altaf1 and Zahra Naeem1
...vitro. Firstly, cell cytotoxicity of ivermectin in the Vero cells was determined using MTT assay. The antiviral activity of ivermectin was determined using viral inhibition assays and median tissue culture infectious dose (TCID50). The results showed that 2.5 µM concentration of the ivermectin is non-toxic to the Vero cells. At this concentration, PPRV titre was significantly reduced (p < 0.001) by two log to 103.0 TCI...
Asmaa A.M. Abd El-Ghani *; Ahmed B.M.; Abdel-Raouf A. ; Nagwa S. Ata* and H.A. Hussein
...v>...
Hanaa H. A. Gomaa1; Entsar A. A. Nassar2; K. A. El-Dougdoug3
...1 and COXB4 viruses. The cytotoxicity of different milk thistle methanolic extracts in Vero cells did not show any morphological difference compared with control. Antiviral affects of milk the most potent methanolic solutions of milk thistle that have antiviral activity with different degrees, line 32 with (HAV), line 9 with (COXB4) and, line 28 with (HSV1)....
Neda Amani1, Mehrdad Shariati1, Rahim Ahmadi2,3*, Mokhtar Mokhtari1 and Saeed Khatamsaz1
...tosterone enanthate. The cytotoxic effect of testosterone enanthate was measured using MTT assay method. iNOS, MMP9, caspase-3, -8 and -9 expression level were evaluated by real-time PCR and the caspases activity was measured by ELYSA method. Flow cytometry was used to detect and quantify the level of apoptosis in cell lines. The data were statistically analyzed between groups using ANOVA and student’s t-test. Decreased viability was observed in AGS and ...
Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4
...CH/C5 showed the highest cytotoxic effect and reduced survival rate of osteosarcoma cells to 75.68% after 24 h. The subsequent increase of CeO2 concentration was also effective but its effect was less than CL/CH/C5 sample. Finally, cerium-doped nanobioactive glass/collagen/chitosan composites scaffolds were exhibited good biocompatibility on normal cells and increased cytotoxicity on cancer osteosaroma cells.
Afsheen Noman Saddar1, Anila Naz Soomro2*, Sadaf Tabasum Qureshi1, Syeda Saleha Hassaney1 and Mukhtiar Ahmed Mahar2
...mparison of brine shrimp cytotoxicity assay revealed noticeable deviations from the both positive and negative controls. Alive percentage of nauplii observed in the aqueous extract of sweet flag root and radish seed ranged from 0 to 10%, land-calotrops root extract from 0 to 22.5%, peppermint leaves extract from 3 to 47.5% and for red cabbage extract ranged from 0 to 15%. Dose dependent death rate was only observed in red cabbage extract while other plant extr...
Ahlam G. Khalifa1*, Walaa A. Moselhy1, Hanaa M. Mohammed2 and Khaled Abdou1
...tamethrin had a stronger cytotoxic effect on the bone marrow cells than the CS/DM Nps and S/DM Nps groups. It was concluded that these innovative nanoformulations of DM were less toxic, which would lower health risks and boost application safety.
Keywords | Cytotoxic, Chromosome aberrations, Loaded, Nanopesticides, Safety
...
Yang Yang1,2, Hong Zhou3, Ji Shao1,2, Xinyi Zhang1,2, Pengpeng Xia1,2, Mingxu Zhou4,5, Qiangde Duan1,2 and Guoqiang Zhu1,2*
...on, adherence, invasion, cytotoxicity, and so on. The results showed that hcp1 of the T6SS did not influence bacterial growth and biofilm formation. In F18ab△hcp1, bacterial motility, adherence and invasion towards the intestinal porcine enterocyte cells (IPEC-J2) were reduced significantly, and the cytotoxicity decreased. The results of RT-PCR showed that in F18ab△hcp1, the flagella gene flic and type I fimbriae fimA we...
Khuzeema Tanveer1, Muhammad Shahid Mahmood1,*, Muhammad Ashraf1 and Ahrar Khan2
...rease in red blood cells cytotoxicity caused by bacterial treated or untreated dye solutions. The current study suggests that some of the bacteria have the potential to degrade a number of textile industry dyes and could be exploited for xenobiotic removal....
Xinjun Zhang1,2, Pengyong Wang1,2, Huimin Ju1,2, Yanhong Wang1,2, Yang Yang1,2* and Guoqiang Zhu1,2*
...C in invading T24 cells, cytotoxicity, immune response and synthesis of proinflammatory cytokines were discussed. The results showed that fimH was 100% detected, followed by iut, iroN, and focA, with the detection rates of 71.4, 57.1 and 57.1% respectively; while papG allele I, papG allele I’, papG allele II, papG allele III, cdtI, sat, sfaS, iha and sat were not detected. Most of the isolated UPEC strains have a strong virulence on T24 cells and could i...
Deepesh Kumar Bhuptani1, Atta Hussain Shah1*, Gul Bahar Khaskheli1, Zubair Ahmed Laghari2, Ghulam Shabir Bahram1, Muneer Ahmed Jamali1, Tarique Ahmed Khokhar1
...s lethal but it is still cytotoxic. The present study was conducted on prevalence and quantification of AFM1 in raw buffalo milk in Sindh province. A total of three hundred and fifteen raw milk samples (n=315) from dairy farms of three regions in Sindh province i.e., southern (Karachi, Thatta, Hyderabad), central (Mirpurkhas, Umerkot, Shaheed Benazirabad) and northern (Naushahro Feroze, Sukkur and Larkana) were collected and analyzed using commercial ELISA kit...
Mehroze Amin1, Afifa Yaqub1, Qindeel Fatima1, Rabail Hassan Toor1,3 and Abdul Rauf Shakoori1,2*
...HEK2093. Analysis of the cytotoxicity and proliferation parameters showed that higher concentrations of metformin were cytotoxic for the breast cancer cells with EC50 of 22.75µM. Analysis of expression of p53 and the interacting partner genes showed that metformin upregulated p53 expression in a dose-dependent manner, while the expression of interacting partners is downregulated indicating that metformin exhibits the a...
Nuzhat Munawar1, Abrar Hussain1, Imran Sajid2, Zahra Noreen1, Muhammad Aslam3*, Zeeshan Rehman1 and Aamir Ali3,4
...aluated using micro well cytotoxicity assay against brine shrimp larvae where chloroform extract showed minimal cytotoxic level (23.37%) while acetone extract showed maximum cytotoxicity (65%)....
Hesham A. Mahran1,2*, Shawky M Aboelhadid3, Saad S. Alqahtani4,5, Mohamed Z. Sayed Ahmed4,5
Ubaida Hussain1,2*, Amtul Jamil Sami1*, Shazia Rafique3*, Muhammad Idrees khan3 and Ahmad Ali Shahid3
...s work was to assess the cytotoxicity and in vitro inhibitory activities of local medicinal plant Azadirachta indica (A. indica) extracts on expression of NS3 and NS5A nonstructural proteins of HCV 3a genotype. Owing to the fact that HCV patients can develop HCC, plant extract has also been tested for cytotoxic activity. Colorimetric analysis was done to determine the vitality of HepG 2 cells for 24 h after treatment with me...
Arshad Javaid1*, Iqra Haider Khan1, Malik F. H. Ferdosi2, Aneela Anwar3 and Mujahid Manzoor4
...ncer, anti-inflammatory, cytotoxicity, insecticidal and insect repellant properties as reported in the literature. ...
Mustafa M. Khalaf*, Rana A. Salih
...cyclophosphamide’s cytotoxic effects was investigated in this study after showing the results concluded when used quercetin as protective. ...
ZAMARA MASOOD1, FAROOQ SALEEM1, SARFRAZ AHMAD2, TALHA JAMSHAID3, IMRAN WAHEED4,
MUHAMMAD AZEEM1, UMAR FAROOQ GOHAR5 & HAFIZ MUHAMMAD ARSALAN6, 7*
Ghusoon Hasan Jadaan1*, Khalisa K. Khudair2
...powder, SiO2NPs cause cytotoxicity and genotoxicity in a variety of cell lines due to oxidative stress. Curcumin Phytosome has antioxidant effects. We aimed to determine how silicon dioxide nanoparticle (SiO2NPs) exposure to female rats affected the oxidative state. Ultraviolet-visible (UV-VIS), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopy were used to investigate SiO2NPs and Curcumin Phytosome nanoparticles (CPNPs)...
Faisal Alzahani1* and Mohammed Abu El-Magd2*
...d a significantly higher cytotoxic effect for CAPE on HepG2 cells than CNPs. The combined therapy with CAPE and CNPs exhibited significantly higher expression of the apoptotic Bax gene and lower expression of the antiapoptotic Bcl2 gene than treatment with each alone. CAPE and CNPs co-treatment also inhibited global DNA methylation levels and downregulated the expression of DNA methylation-related genes (DNMT1 and Ube2e2) in HepG2 compared to cells treated wit...
Nael Abutaha, Fahd A. AL-Mekhlafi*, Khalid Elfaki Ibrahim,
Mohammed S. Al-Khalifa and Mohamed A. Wadaan
...pectrometry (GC-MS). The cytotoxic effect was also evaluated using normal human umbilical endothelial cells (HUV-EC). The larvicidal assay was performed on the third instar stage as recommended by WHO. The phytochemical analysis showed the presence of alkaloids, sterols, and phenolic compounds. The GC-MS analysis revealed the presence of 5 compounds, of which α-Copaene constituted 61.95% of the extract. The hexane extract showed good larvicidal activity,...
Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin2
Qurrat ul Ain Shafique1, Sana Batool2, Hanfa Ashfaq2, Asima Tayyab2, Roquyya Gul3 and Mahjabeen Saleem1*
... of ~95%. In vitro cytotoxic potential of IL-2 on HepG-2 and MCF-7 cell lines revealed that it possess sufficient cytotoxic potential and can inhibit the growth of these cell lines directly. Thus, refolded IL-2 had activity identical to that of authentic IL-2 and enhanced the anti-tumor activity of HepG-2 as compared to MCF-7 cells. These conclusions suggest the potential use of the refolded cytokine as immunotherapeut...
Shabnam Javed1 and Amna Shoaib2
...esent study examined the cytotoxic activities
of S. tomentosa using methanolic extracts and fractions (n-hexane, dichloromethane, ethyl
acetate and water) against three cancer cell lines (lung A-549, hepatocellular HepG2and
urinary bladder EI-138). Cytotoxic assays were carried out with five concentrations (0.05,
0.01, 0.05, 0.1 and 0.5 mg mL-1) of methanolic extract and its su...
Hafiz Muhammad Farooq Yaqub1, Sehrish Firyal1, Ali Raza Awan1, Muhammad Tayyab1, Rashid Saif2, Muti ur Rehman3, Muhammad Wasim1*
...dy size and responses to cytotoxic and other therapeutics. Small noncoding RNA having length of 22 base pair (bp) are called micro-RNA (miRNA), that are processed by Dicer from precursors with a characteristic hairpin secondary structure. miRNAs can regulate gene expression at the post-transcriptional level by binding to the 3′ untranslated region (UTR) of target mRNAs. In this study dog mammary tumor samples were collected to investigate the expression ...
Hafiz Muhammad Farooq Yaqub1, Sehrish Firyal1, Ali Raza Awan1, Muhammad Tayyab1, Rashid Saif2, Muti ur Rehman3, Muhammad Wasim1*
...dy size and responses to cytotoxic and other therapeutics. Small noncoding RNA having length of 22 base pair (bp) are called micro-RNA (miRNA), that are processed by Dicer from precursors with a characteristic hairpin secondary structure. miRNAs can regulate gene expression at the post-transcriptional level by binding to the 3′ untranslated region (UTR) of target mRNAs. In this study dog mammary tumor samples were collected to investigate the expression ...
Hina Yasin1,2*, Shaukat Mahmud2, Hina Abrar1, Kaneez Fatima3, Rabia Bushra1 and Saima Zahid2
...reened for analgesic and cytotoxic activities using animal models for the safe and effective utilization of plant material. The leaves were soaked in methanol and successively fractionated using n-hexane, chloroform, ethyl acetate and water. Mentioned biological activities were determined in different groups of wistar strain mice by administering various leaves extracts in differential dose pattern (81, 54 and 27 mg/kg). Cytotoxic
Ashraf Samir Hakim1*, Doaa Diab Khalaf1, Engy Farahat1, Mohammed Darwish Mohammed1, Wahid Hussein El-Dabae1, Khaled Abd El-Hamid Abd El‑Razik2, Amany Nabil Dapgh3, Ehab Ali Fouad4, Hussein Ahmed Abuelhag1
...o red binding, Vero cell cytotoxicity. The antibiotics resistance pattern was assessed by disk diffusion test. Also, the E. coli isolates were molecularly confirmed and screened for presence of 3 virulence genes (fimH, iss and iutA) as well as blaNDM-1gene which responsible for carbapenem resistance. The data were statistically analyzed using Chi-square (χ2) via (SPSS) version 20.0 software. Our results revealed sixty three E. coli isolates were obtained (...
Sherine Abbas1, Heba M.A. Abdelrazek2*, Eman M. Abouelhassan3, Nayrouz A. Attia4, Haneen M. Abdelnabi4, Seif El-Eslam E. Salah4, Abdelrahman M. Zaki4, Mohamed A. Abo- Zaid4, Nadia A. El-Fahla5
...eem leaves extract has a cytotoxic effect that affects the histo-architecture of lymphoid organs, promoted inflammatory markers and the mononuclear cells’ DNA damage that seemed to be as a result of neem extract oxidative stress. This effect could be implemented on both human and animals with future prospective studies suggestion to estimate the dose dependent effect of prenatal neem extract exposure.
Keywords: Hemato...
