Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
...cated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these strains have shown homology with previously characterized strains from Paki...
Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad
British Journal of Virology
...cated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these strains have shown homology with previously characterized strains from Paki...
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
...cated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% nucleotide sequences similarity was determined between the representative strains. Furthermore, these strains have shown homology with previously characterized strains from Paki...
Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2
...minants, and to identify serotypes present in the country....
Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*
...70 log10 (for serotype Asia 1), 1.99 log10 (for serotype A) and 1.53 log10 (for serotype O) in ELISA while in SNT, antibody titers were found to be 1.86 log10 (for serotype Asia 1), 2.26 log10 (for serotype A), 2.11 log10 (for
Moustafa Kardjadj
...he disease. Currently, 3 serotypes of FMD virus (O, A and SAT-2) and 06 lineage are circulating in the North Africa, of which serotype O is responsible for most of the outbreaks. However, the rapid spread of SAT2 and other exotic FMDV lineages in Libya and Egypt demonstrated the need for a robust surveillance system to detect and respond effectively to exotic infections. Emerge...
John G. Bruno
...een the various captured serotypes, each of the anti-LPS aptamer candidates showed a preference for its cognate E. coli serotype. Results suggest that these aptamers may be useful for enriching specific E. coli pathogens during aptamer-magnetic separation in food samples, leading to a greater ability to detect these serotypes if coupled with genetic identification techniques such as PCR or...
Farkhanda Manzoor1, Iram Amin2,*, Muhammad Shahid2,Samia Afzal2,Hania Ramzan1, Tahir Rehman Samiullah2 and Muhammad Idrees3
...2 was the most prevalent serotype. DENV-1 was not found in any pool. While all pools of Aedes albopictus were negative for dengue virus. Our study showed that the main cause of spreading of dengue virus was Aedes aegypti. Serotype DENV-2 was dominant in the field collected larvae of Aedes aegypti in Lahore and Sheikhupura, Pakistan.
...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...sentative of a velogenic serotype. Moreover, based on F and HN gene nucleotide sequence analysis, the isolate clustered as genotype VII closely associated to those reported from Indonesia. Further sequence analysis of hyper-variable region within F gene revealed its clustering to sub-genotype VIIe. The inferred residue analysis of the virus revealed a number of substitution mutations in the structural and functional domains when compared to the representative ...
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
... cDNA of the four dengue serotypes. Six out of 35 (17.14%) adult mosquito pools were found positive for dengue virus with type-2 (77.77 %) and type-3 (11.11 %) while a single (11.11%) pool of concurrent infection with type 2 & 3 was also detected. The adult Ae. aegypti belonging to Shuba Bazaar showed 33% positivity for DENV with minimum infection rate (MIR) 11.1 while that of Wazir Bagh showed 16.67% positivity with MIR 5.5. Similarly, the Ae. a...
Eman M. Farghaly1, Ahmed Samy1,2*, Heba Roshdy
...gle infection. Different serotypes of E.coli and Salmonella could be recovered from dead birds however E.coli (O78) and S.enteritidis were recovered mainly from heart and liver. The recovered E.coli, S.aureus and P. haemolytica isolates recovered from mixed infection cases showed (57.1-100%) resistance to highly important antibiotic group (Doxycycline, Tetracycline, Trimethoprim sulfa methoxazole and Chloramphenicol) and showed dissimilar pattern of resistance...
Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1
...nsidered. Four different serotypes were identified with Salmonella Llandoff having the highest isolation rate in all the matrices sampled (58.8%), followed by, S. Kentucky (17.7%), S. Schwarzengrund and S. Havana had the lowest isolation rate (11.8%). S. Llandoff was isolated in all the matrices and was distributed across the three LBM whereas the other less frequent serovars had a more circumscribed distribution. Resistance to Methicillin, Penicillin, Erythro...
Muhammad Shahid1, Iram Amin1,*, Samia Afzal1, Zareen Fatima1, Sadia Zahid1, Usman Ashraf1 and Muhammad Idrees2
...bopictus having five serotypes 1-5 and infecting about 100 million people each year. To address dengue outbreak 2013 in Pakistan we aimed to conduct a comprehensive study at molecular level to determine the main causative serotype of 2013 DENV outbreak in Pakistan. Overall 703 serologically positive suspected patients from different major health centers of Pakistan were registered in present study of which 214 were femal...
Rabia Tahir1*, Khushi Muhammad1, Masood Rabbani1, Muti-u-Rehman Khan2, Farah Khan1, Hassan Bin Aslam1 and Arslan Mehboob3
...tern of avian adenovirus serotype 4 (AAV-4) causing hydropericardium syndrome (HPS) by transovarian route. For this purpose, liver and spleen samples (n=90) were collected from day-old-chicks derived from breeders at 14, 21 and 30 days of post-infection with AAV-4. The presence of AAV-4 DNA was detected through PCR. Chicks from virus-challenged breeders failed to show a clear and expected PCR positivity. The connotations derived from these findings are that ve...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*
.../i>). Moreover O-antigen serotype was tested by slide agglutination test and a mouse model was built to assess the lethality of the E.coli isolates through subcutaneous-infection. Out of 81 E.coli isolates, the most prevalent gene detected was ompA (90.12%), followed by ETT2 (69.14%), irp2 (54.32%), EAST1 (46.91%), CS31A (41.98%), estB (19.75%), eaeA (14.81%), estA (12.35%) and K88 (1...
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4
...her vNDV, E. coli serotype O78 or both agents simultaneously or 3 days apart. The birds were clinically observed and swabbed daily. They were killed at 4 and 14 days after single or dual inoculations and were inspected for gross lesions. Samples of the respiratory organs (trachea, lungs, and air sacs) were taken for histological analyses. All the infected subjects showed clinical signs of varying severity. Co-infected groups showed the most obvious clin...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...rnaviridae. It has seven serotypes with no cross protection among these. FMD is responsible for heavy economic losses in cattle and buffalo. So rapid and accurate diagnosis of FMD is uttermost need of this time. Several lab.techniques are developed for rapid detection of FMD but these are expensive and time consuming. Elisa, cell culture, Reverse Transcriptase (RT-PCR) and real time PCR need specific high quality equipments in lab. Whereas Lamp PCR is simple a...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...f fowl adenovirus (FAdV) serotype is of most importance in epidemiological studies of disease outbreak and the adaptation of vaccine strategies. In spite of appropriate administration of vaccination, FAdV outbreaks have been reported and caused significant losses in poultry flocks throughout Pakistan in recent years. To identify the serotype and gain a better understanding of the genetic properties of the FAdV strains respon...
Bo Wu1, Hui Zhang2, Kun Li2, Khalid Mehmood2,7, Yan Zhao1, Bin Jiang1, Chengjun Xue3, Muhammad Tariq Javed6, Fazul Nabi2, Zhaoqing Han5,* and Houqiang Luo4,*
... program optimization of serotype O of FMD through ELISA and IHA in pigs in Zhejiang. A total of 368 serum samples were collected from June to July in 2016. Out of these samples, 23 (6.79%, 95% CI 4.4-9.9) pigs were found positive for FMD antibodies with the further distribution of 9.09% (95% CI 4.6–15.7), 6.90% (95% CI 2.6–14.4), 5.49%, (95% CI 1.8-12.4) and 4.35% (95% CI 0.9-12.2) from Wenzhou, Lishui, Jinhua and Ningbo counties, respectively. Fo...
Imran Taj1,*, Ferhat Abbas1, Zafar Ahmad1, Mohammad Kamran Taj1, Deedar Ahmad2, Zahoor Ahmed2, Ghulam Mohammad2, Ajaz-ul-Haq1, Farooq Shahzad1 and Sabiha Azam3
Waqas Ali* and Mudasser Habib
...nt study, 336 strains of serotype O (n = 178), A (n = 82) and Asia 1 (n = 76) were downloaded from NCBI database that mainly belonging to Pakistan, along with three vaccinal strains. Phylogenetic analysis (maximum likelihood) showed that lineages Pan Asia II, An Iran-05, and Group VII within serotype O, A and Asia 1 are causing majority of the outbreaks in the Pakistan during recent years. Moreover, only one residue of VP1 r...
Omer Baris Ince*
...ive vaccines of suitable serotype and measures are taken in order to reduce the prevalence of the disease. In this study, blood samples were collected from clinically healthy sheep and bovine in the surrounding region of the southeast of Konya province that were classified as non-vaccinated, single-vaccinated, multiple vaccinated, aged 0-1 and 1-3 and male and female starting from the third month following the vaccination. In the study aimed to show the diseas...
Muhammad Shahid1, Iram Amin1, Samia Afzal1,*, Zareen Fatima1 and Muhammad Idrees2
...ained the main causative serotype of the outbreak of dengue viral infection, started in the commencement of year 2013 and continues to infect Pakistani population till now.
...
Nida Zia1, *, Ayesha Maqbool2, Muhammad Safdar3, Umm-I-Habiba1, Altaf Mehmood4, Muhammad Usman5, Zahid Iqbal6, Javed Iqbal6, Shahid Mehmood6, Amanullah Khan7 and Sajid Umar8
... the FAdV- C species and serotyped as FAdV-4 and showed close proximity at the nucleotide level. The other cluster containing three strains belonged to the FAdV-D species and serotyped as FAdV-11. Furthermore, the sequencing analysis of detected field strains revealed the high similarity and close clustering with FAdV-4 and FAdV-11 strains isolated from neighboring countries, suggesting geographic and temporal relationships ...
Naglaa M. Hagag1*, Mervat E. Hamdy1, Mary A. Sargious1, Sara M. Elnomrosy1, Nahed A. Ahmed1, Ayman A. Hamed1, Ahmed R. Habashi1, Essam I. Ibrahiem1, Mahmoud A. Abdel-Hakim2 and Momtaz A. Shahein1
...erization indicates that serotype SAT2 is widespread (in 96 cases), while only four cases of Serotype O were detected. Phylogenetic analysis of the study sequences indicates that the circulating FMDV serotype SAT2 viruses were homogeneous and related to Topotype VII. Importantly, the newly emerged viruses were closely related to strains isolated from Libya in 2012 (Topotype VII, lineage 3)...
Parakriti Gupta1, Kapil Goyal2 and Mini Pritam Singh3*
Hepatitis E Virus and Zoonosis
...h the virus has only one serotype, 8 genotypes have been documented. Out of these, HEV 1 and 2 have been reported from developing countries and are transmitted mainly through faeco-oral route while HEV 3 and 4 are reported from developed countries and the infection is acquired by humans due to the eating of undercooked pigs, deer and wild boar meat. Recently HEV genotypes 5 and 6 have been reported in Japanese wild boars while HEV genotypes 7 and 8 have been r...
Mumtaz Ali Khan1, Sher Bahadar Khan2, Shakoor Ahmad2, Irshad Ahmad3, Kashif Prince1*, Ghazunfar Rashid1, Mahboob Ali1, Imdad Ullah Khan4, Asad Ullah5, Naimat Ullah4, Muhammad Shoaib2 and Said Sajjad Ali Shah2
... infected with different serotypes (A, B, and C) were categorized into two groups healthy and diseased to compare the hematological and biochemical parameters. Hematobiochemical analysis indicated that mean erythrocyte count (RBC) and hemoglobin levels decreased while the mean leukocytes (WBC), platelets, packet cell volume (PCV) and total bilirubin levels increased significantly (P<0.05) in sheep infected with Clostridium perfringens type A. Sheep i...
Rahma Mohamed, Sara Nader, Dalia Hamza and Maha A. Sabry*
...r 2 C. neoformans serotype A (3.8%) isolates identified in healthy and diseased donkeys. Four C. gattii and C. neoformans isolates demonstrated higher laccase (LAC1) genes among the identified virulence factors. While capsular associated protein (CAP59) gene identified alone or associated with LAC1 gene in the other 2 C. gattii isolates. This study underlines a potential association of those fungi with human dis...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...nd also copying specific serotypes O, A and SAT2 nucleotide sequence. In this work FMDV serotype O, A and SAT2 were isolated from cattle clinical samples (foot lesions). RNA extracted from clinical samples was subjected to reverse transcriptase polymerase chain reaction( Rt. PCR) nucleotide of FMDV ID and 3D genes were determined using standard automated sequencing technique. Phylogenetic analysis of FMD / SAT-2 /3D/Egypt/Sh...
Fawzy Rania1, AboElkhair M.1, Bazid A.M.1, Sultan H.2, Hussien A.H.3
...ulation of different IBV serotypes in broiler sectors. Regular monitoring for evolution, epidemiology and genotyping of Egyptian IBV field strains is very important for screening the emergence of new variant strains as well as evaluating the existing vaccination programs....
Hala K. Abdelmegeed 1, Eman M. Abohatab 1, Khattab,O. M. 1 , Salem, S.A.H. 1 , Arafa, A. 2, Nashwa M. Helmy 3
...ype A and (3) serotype SAT2 the samples collected from outbreaks in period November 2013 –July 2014 the season Autumn and winter for forming an Epidemiological map for 10 governorates of the circulating strains during this period
...
Nashwa M. Helmy1 and Ahmed S. A.2
...animals. There are seven serotypes of FMD virus (FMDV), namely O, A, C, SAT 1, SAT
2, SAT 3 and Asia 1. Tests for antibodies of FMDV nonstructural proteins (NSPs) are
useful in providing evidence of previous or current viral replication in the host,
irrespective of vaccination status. NSPs, unlike structural proteins are highly conserved
and therefore are not serotype specific ...
Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3
...div>
onwards. FMDV serotype (O) was the most prevalent until serotype (A) appeared in 2006 then
during April and May 2012, six outbreaks of FMD serotype (SAT 2) were reported in Egyptian
governorates. This study based on evaluation of vaccinated animals by detection of antibodies
against serotypes of FMDV (A),(O)...
Lamya A. F. Ateya1 , Said. A .Ahmed2, Khamees K.S. Ashraf3, Heba A. Abdel-Hady4
...%), 69(37.1%) 10 WPV for serotype A.For serotype (O):55(29.5%) ,58(31.2%)2WPVand 60(32.3%), 62(33.3%) 4WPV and 62(33.3.5%) , 62(33.3%) 8 WPV 60 (32.3%),63(33.9%)10 WPV.For serotype (SAT2):30(.16.1%), 22(11.8%) 2WPV and 39(20.9%), 41(22%)4WPV and 37(19.9%),41(22%) 8/ WPV/ and 27(14.5%), 35(18.8%) 10 weeks post vaccination. Conclusion: vaccines are a fundamental strategies aimed at global co...
Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1
.../014p with Massachusetts serotypes. The commercial H120 vaccine conferred partial protection against proventriculitis induced by Egypt/Qal/014p. Conclusion: IBV strains exhibiting proventriculitis were found co-circulating in broiler chickens in Egypt and optimum control of IBV in Egypt require preparation of vaccine from indigenous isolates beside periodic evaluation of cross-protective capabilities of such vaccine....
Muhammad G. Khodary1, Ayman H. El-Deeb2, Mohamed M. Emara2, Othman E. Othman1, Hussein A. Hussein2
...
samples belonged to serotype O of FMDV. Sequencing and phylogenetic analysis of 6 representative
samples clustered the detected viruses with O topotype East Africa-3 (EA-3) viruses.
Conclusion: The newly identified 2016 viruses clustered in distinct clade in the phylogenetic tree
other than the serotype O viruses isolated in previous outbreaks in Egypt, indicating the likelihood of
Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2
...of all seven
serotypes of FMDV. The positive-resulted samples were then tested using conventional PCR and pan
FMD primers. The same samples were tested again using serotype-specific primers to amplify the VP1
coding region and the products were sequenced for phylogenetic analysis.
Results: FMDV RNA was detected in eight out of the 12 samples tested using real time RT-PCR.
...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi
...ty with VAR2
serotype present in Egypt.
Conclusion: VAR2-IBV is circulating among chickens demonstrating mortalities which affect poultry
industry in Lebanon.
... 
Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2*
...olates belong to somatic serotypes 1, 3, and 12 with a percentage of 20%, 10%, and 10% respectively, while other isolates were untypable. All the recovered isolates were further subjected to multiplex PCR screening of some common virulence genes and revealed the presence of pfhA, hgbB, tbpA, toxA, sodA, ptfA, sodC, nanB, and oma87 with percentages of 30%, 60%, 10%, 100%, 70%, 90%, 90%, 90%, and 100% respectively. Antibiotic susceptibility tests of the recover...
Asmaa G. Mubarak1*, Mona M. Mustafa2, Mohamed W. Abdel-Azeem3, Dina N. Ali2
... of different Salmonella serotypes to hospitalized patients in Assiut Governorate, Egypt, as well as, to assess their pathogenic potential and antimicrobial resistance. Out of 150 chicken meals collected randomly from various restaurants and food shops including, shish-tawook, pane, and shawerma (50 for each), 10% were contaminated with Salmonella with the acquisition of shish-tawook (14%). On the other hand, 100 hand swabs that were assembled from food h...
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
...trol due to its multiple serotypes. The disease could be prevented by rapid diagnosis either molecular or serological test. However, the later test is inexpensive such as heamagglutination inhibition test (HI), but IBV fail to give Heamagglutination (HA) reaction without pretreatment. Therefore, we designed this study for preparation of IBV antigen by treating with different enzymes for HA reaction. IBV local isolates were characterized by SDS-PAGE and RT-PCR....
Bikash Puri1, Anil K. Tiwary2, Bharata Regmi3,4, Dinesh K. Singh1, Doj R. Khanal5 and Manoj K. Shah3*
...the species level and to serotype the BTV prevalent in the study areas....
Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1
...ential of M. haemolytica serotype A:1 OMVs (MH-OMVs). Fifteen calves were divided randomly into three groups of five calves and immunized with single-dose and booster-dose of 0.15 mg/ml vesicle preparation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay of the partially purified MH-OMVs revealed approximately ten protein bands ranging from 25 to 104 kDa. Hemagglutination inhibition (HI) assay confirmed that the immune response was s...
Sania Subhan Qureshi*,1, Baitullah Khan1, Shahid Khan1, Hanif Ur Rahman1 and Muhammad Subhan Qureshi2
...is caused by 7 different serotypes of FMD virus having different virulence. To compare the virulence of various FMD serotypes the study was designed to propagate the virus on BHK-21 cell line. The results revealed that serotype ‘A’ virus virulence is more as compared to serotype ‘O’ and ‘Asia-1’. Although less virulenc...
Luu Huynh Anh1, Huynh Tan Loc2, Nguyen Hong Xuan3, Le Minh Thanh1, Trinh Thi Hong Mo4, Ly Thi Thu Lan5, Nguyen Trong Ngu1*
...against Escherichia coli serotype O6 infected in chickens. A total of 420 broilers were randomly assigned to seven treatment groups. The negative control (NC) birds received no E. coli or phages, whereas the positive control (PC) birds were infected with E. coli only. The NC+MHH6 and NC+PR2 treatments received 109 pfu/ml of phages MHH6 or PR2, respectively; whereas the PC+MHH6, PC+PR2, and PC+MHH6PR2 groups were infected with 107.1 cfu/ml of E. coli strain O6 ...
Nishant Shah1*, Rakesh Kumar Yadav1, Anil Gautam1*, Shambhu Shah1 and Mohan Kumar Gupta2
...oli of strain χ7122 (serotype 078: K80:H9) at pathogenicity amount (9.4 x 105 cfu E. coli per bird) was done and morbidity and mortality rate was also recorded. The significantly minimum ammonia (ppm) was recorded in CRH (30.53±0.77); however, the maximum ammonia (ppm) was recorded in RH (49.78±0.42) with that compared to SD (33.42±0.25) and RS (43.22±0.56). Similarly, the mortality rate due to colisepticemia was recorded minimu...
Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6
...h Mannheimia haemolytica serotype A2 during rainy and hot seasons. Twenty-four healthy female, non-pregnant does were used, and divided into two equal groups, 12 does were further allocated into 3 groups (n=4), control, non-vaccinated and vaccinated in each season. After acclimatization and synchronization procedure, the vaccinated group was administered with 2 mL of alum-precipitated pasteurellosis vaccine while non-vaccinated and control groups were administ...
Gamilat A. Elsaid1, Weam Mohamed Baher1*, Eman Shukry1, Abeer E. Abd El. Ghafar2, Marwa Shalaby1
.... The identified E. coli serotypes were E. coli O2:H6, E. coli O26:H11, E. coli O55:H7, E. coli O78:H-, E. coli O111:H4, and E. coli O119: H. All isolated harbored 16S rRNA specific gene for E. coli. The expression of shiga toxin coding genes indicated that E. coli O2:H6, E. coli O111:H4, and E. coli O119:H harbored only stx2. E. coli O26:H11 and E. coli O55:H7 harbored both stx1, and stx2; however, E. coli O78:H- did not harbor any of the tested genes. The us...
Zohair S. Mulla1*, Mostafa M. Abdelhafeez2
...ther identified into six serotypes, namely E. coli O2:H6, O26:H11, O55:H7, O78:H-, O86:H11, and O119:H4. Detection of shiga toxin producing genes (stx1 and stx2) in the recovered serotypes was followed using multiplex PCR. A significant decrease in the microbial loads was recorded after dipping of the formulated ground beef as meat balls in the acid solutions, particularly at the acid cocktail 2%. To the best of our knowledg...
Gawhara Ahmed-Abdelmonem1*, Zeinab Aboezz2, Ahmed Habashi1, Saad Sharawi2
...ular characterization of serotype A FMDV that has been involved in the latest FMD outbreaks in Egypt. Thirty-six samples (26 blood and 10 oral epithelial tissue samples) were obtained from suspected cattle in three Egyptian governorates during 2019-2020. The samples were screened for FMDV by means of real‑time RT‑PCR that showed nearly 86% (n=31) of the examined samples to be FMDV positive. Virus isolation was carried out on ten of the FMDV positive sample...
Eman H. Abotalp, Sahar R. Mohamed, Jakeen K. El Jakee
...ized with unsafe E. coli serotypes and the antibiotic resistance in these E. coli isolates. A total of 129 rectal swabs were gathered from apparent healthy and diarrheic canines and felines. By using Vitek2 compact system, 42 E. coli isolates (32.6%) were resulted from canines (24%) and felines (44.4%) rectal swabs. E. coli were serotyped to: O8, O25, O26, O28, O36, O55, O78, O86, O111, O114, O125, O127, O128 and O157. ...
Hassan A. Aidaros, Eman M. Hafez, Halla E.K. El Bahgy*
...s against field isolated serotypes of E. coli, Pasteurella multocida, Campylobacter jejuni, and Staphylococcus aureus from chicken and duck farms production at a titer of 3×106 CFU (Colony Forming Unit) /cm2 in the absence and presence of organic matter (O.M). The results showed that the efficiency of disinfectants was significantly increase with high concentration and long contact time. The Prophyl 2000® was the most powerful disinfectant against fi...
H. A. Aidaros1*, S. S. Khalafallah1, M. S. Diab2, Nehal K. Alm Eldin2, Halla E.K. El Bahgy1
...70% & 60% of E. coli serotypes, respectively. The application of good biosecurity programs including strict measures in poultry farms is the preferable method to reduce the risk of pathogenic bacteria and reduce the use of antibiotics.
Keywords | Poultry farms, Hygiene, Enterobacteriaceae, Antimicrobial Resistance, Resistance gens.
...
Chunlan Shan1, Chaoying Liu1, Qin Lu1, Guowen Fu2, Syed Aftab Hussain Shah3, Rana Waseem Akhtar4, Ru Zhao2, Libo Gao2, Chang Liu2, Shushu Miao2, Hongdan Wang2 and Hong Gao2*
...hich 44 E. coli superior serotype strains were isolated and identified from Yunnan Saba pigs. The genomic DNA of all isolated superior serotypes of E. coli was obtained. Five major high pathogenicity island (HPI) structural genes (irp1, irp2, irp3, irp4 and irp5) were cloned, sequenced and referenced with GenBank database. The sequence identities of irp1, irp2, irp3, irp4 and irp5 with GenBank were 98 %, 99 %, 99 %, 98 %, an...
Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3
...ng the recovered E. coli serotypes, the serotype O111:K58 (B4) was the most prevalent (38.2%). An inactivated polyvalent autogenous mastitis vaccine was prepared from the most encountered bacterial pathogens, namely, E. coli, S. aureus, Str. agalactiae and Str. dysgalactiae. The selected strains for vaccine preparation were inactivated with a predetermined minimal lethal dose of gamma radiation (4krad kGy/min) and the bacte...
Muhammad Mohsin Abbas1*, Sajjad Ur Rahman1, Muhammad Abubakar2, Muhammad Imran Arshad1 and Khurram Ashfaq3
...n of the prevalent virus serotypes in the area. The current cross-sectional study was conducted in the Bahawalpur district of Punjab, Pakistan, with the purpose to determine the prevalence of FMD virus serotypes Asia 1, O and A in bovines. A total of 838 bovine sera samples were tested through Solid phase competitive enzyme-linked immunosorbent assay (SPC-ELISA), based on structural proteins at the National Veterinary Labora...
Sar Zamin Khan1*, Muhammad Waqas1 and Sagar M. Goyal2
...uctive systems. Multiple serotypes of IBV have emerged due to the high rate of mutation and genetic recombination, which has made it difficult to control the disease. Certain serotypes have disappeared because of the availability and use of vaccines but new serotypes have emerged. A regular watch on the disease including virus variation, prevalence, pathogenesis and development of diagnost...
Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1
...y against non-Salmonella serotypes tested. Latent time period of 15, 15, and 20 min was documented for SEPL01, SEPL13, and SEPL20, respectively, with an average burst size of 110, 32, and 63 PFU CFU-1, respectively. All the three bacteriophages were tolerant of temperature range 4°C-70°C and pH between 3-12, thus providing a broader window of their application throughout the food chain. On chicken breast cuts, phage mix efficiently supported sustained ...
Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.
...he diseased animals were serotyped as FMD serotype O....
Eman, Abo Hatab*; Hussein, HA.; El-Sabagh IM. and Saber, MS.
... characterization of GIO serotype of group A rotaviruses from fecal samples collected from camel farms suffering from diarrhea in Alexandria and Esmalia governorates. After preparation of fecal samples and inoculation on MA 104 cell line for five passages, eight isolates were successfully isolated with a clear and reproducible CPE on the inoculated cells. The isolates were identified antigenically using VP6 monoclonal antibodies (MAbs) based antigens capture E...
Ashraf M. Metwally*, Ausama A. Yousif*k , Iman B. Walaa A. Attia M. Samy * , and Ismail M. Reda *
...ous bursal disease (IBD) serotype 1 viruses continue to cause major economic losses in the Egyptian poultry industry despite the implementation of intensive vaccination programs. A recent increase in IBD related mortality in vaccinated farms prompted this investigation into the genetic character of the circulating IBD virus (IBDV). Bursa and proventriculus samples were RT-PCR tested using novel primers flanking VP2 region coding the two major and two minor hyd...
Seham A. El-Zeedy1, Dalia A. Abd El-Moaty1, H. A. Hussein2 and M. A. Shalaby2
...ates with different IBDV serotype I strains showed that isolate shared three unique a.a residues at positions 222A, 256I, and 294Ile that were found only in vvIBDV as well as the conserved serine rich heptapeptide region. Giza/2000 showed two additional unique a.a residues at positions 220Phe and 321Thr that resulted from two unique nucleotide substitution at positions 609 (A to T) and 911 (G to A). Surprisingly Giza/2000 shared the unique a.a residue at posit...
H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3
...tigenic sites with other serotype-1 IBDV strains. as they cross-reacted in AC-ELISA and AGPT. Although, the epidemiological investigation and antigenic typing by AC-CLISA test as well as pathogenicity study suggested that IBD field isolates are in the majority of highly virulent pathotype producing acute disease with severe clinical picture. The current study presents evidence of two variant isolates existing in commercial broiler and native Baladi farms with ...
Walid Elmonir1*, Dalia Abdeltawab1, Hanem El-Sharkawy2 and Rasha N. Zahran3
...dy aimed to estimate the serotypes diversity, virulence and antimicrobial resistance traits of non-typhoidal Salmonella in commercial and backyard egg production systems in Kafrelsheikh Governorate, Egypt. A total of 610 samples (200 egg shells, 200 egg contents, and 210 environmental samples) were collected from 10 commercial layers farms and 170 samples (50 egg shells, 50 egg contents, and 70 environmental samples) were collected from 10 backyard layers floc...
Ying Zhong1,3,4, Jingni Chen1, Chunping Huang1, Huaiyuan Jin1, Jinlu Huang1, Lining Zhao1, Yi Geng3, Guiping Wang1,4 and Xueqiao Qian1,2*
...anslucent rings, and six serotypes were identified. The regression infection results showed that the lethal rates of the three V. parahaemolyticus strains of serotypes O1:KUT and OUT:KUT to L. vannamei were significantly higher than those of the other serotypes. The three V. parahaemolyticus strains had the same growth characteristics, and they all entered the growth plateau at 4 h after i...
Mohamed Ahmed Abaza1, Amany O. Selim2, Mona Abdallah3, Shimaa A.E. Atwa4, Hala El Daous5, Mona Abd-Allah Abd-Elrehim6, Mohamed M.S. Gaballa7, Reda R. Fathy1*
...c at 5 days old. E. coli serotype O6 was the highest prevalent and pathogenic multi drug resistant bacterial strain.The assessment parameters were clinical signs, post-mortem lesions and histopathological picture which showed effective role of ZnO-NPs as bacterial inhibitor in the treated groups compared to control one. Quantitative analysis showed that ZnO-NPs significantly lowered gross lesion scores in the liver, cecum, colon, spleen, heart, and lungs compa...
Ashraf Samir Hakim1*, Doaa Diab Khalaf1, Engy Farahat1, Mohammed Darwish Mohammed1, Wahid Hussein El-Dabae1, Khaled Abd El-Hamid Abd El‑Razik2, Amany Nabil Dapgh3, Ehab Ali Fouad4, Hussein Ahmed Abuelhag1
...ed uropathogenic E. coli serotypes isolated from companion animals and human as well as assessed their virulence determinants which implemented in pathogenesis and severity of infection via phenotypic and molecular methods. Besides that, the study determined their multidrug resistance patterns, particularly the existence of the gene responsible for carbapenem resistance. A cross-sectional study performed on one hundred and ninety two urine samples owned to uri...
Mona F. Shousha1*, Aml M. Ragab2 and Salwa M. Helmy1
... serovar Tranoroa in the serotypes of isolated biochemically identified Salmonella. As per the results of this study, Salmonella isolates demonstrated multidrug-resistant phenotypes, with the highest resistance being against ampicillin, cefoxitin, cefpodoxime, and oxacillin (100%) and then against cefotaxime (80%), ceftazidime (70%), ciprofloxacin, ceftriaxone, and nalidixic acid (60%), including amoxicillin–clavulanic acid (50%). Furthermore, antimicrob...
Thaer W. Enad*, Khalefa A. Mansour
...cal investigation of FMD serotype SAT2 in Iraq buffalo. The study involved 102 buffaloes from two provinces, Wassit and Dhi-Qar. Clinical examination within the herd revealed classic FMD signs, including salivation, oral and nasal mucosal erosions/ulcerations lesions and laminas of affected animals. The proportions of older animals were more likely to show infection (p > 0.05), but there was no significant difference. The female was the predominant sex infe...
Mahmoud Ezzat1, Fatma Youssef2, Ahmed Eid1, Marwa E. Abo Hashem1*
...(2/50). Chicken isolates serotyped as S. Typhimurium (50%) and S. Enteritidis (50%). Human isolates typed as S. Typhimurium (100%, 2/2). All serovars were resistant to clindamycin and erythromycin (100%). Multidrug-resistance (MDR) was detected in SEP, STP, STH by 100%, 80% and 50%, respectively. invA, stn genes were detected by 100% in all serovars. ermB was detected in SEP, STH and STP by 100%, 100% and 80% respectively. aadB gene was detected in SEP, STP an...
Rakhtasha Munir1, Shazia Rafique2*, Iram Amin1,2, Sameen Ahmed2, Ayesha Vajeeha2, Amjad Ali3, Muhammad Shahid2, Muhammad Umer Khan4 and Muhammad Idrees2
...t prevalent dengue virus serotype 2 (DENV-2). The progress of this research would likewise foreshadow advancements of novel vaccines for future counteraction of dengue infection as C protein is highly immunogenic....
