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Rabia Tahir1*, Khushi Muhammad1, Masood Rabbani1, Muti-u-Rehman Khan2, Farah Khan1, Hassan Bin Aslam1 and Arslan Mehboob

...m syndrome (HPS) by transovarian route. For this purpose, liver and spleen samples (n=90) were collected from day-old-chicks derived from breeders at 14, 21 and 30 days of post-infection with AAV-4. The presence of AAV-4 DNA was detected through PCR. Chicks from virus-challenged breeders failed to show a clear and expected PCR positivity. The connotations derived from these findings are that vertical transmission via transovarian
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool3, Fatima Ahmed4, Saiqa Tabassum3,6, Syeda Madiha3, Tahira Perveen3 and Saad Bilal Ahmed5
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
...ole of apoptosis in fish ovarian follicular atresia and postovulatory regression, the pre and postovulatory follicles (POF) of the freshwater teleost, Clarias gariepinus were observed by light microscopy. The germinal vesicle and the cytoplasmic organelles of the oocyte were disintegrated. The theca of atretic mature oocytes was hypertrophied and persisted to form the interstitial cells, whereas the granulosa cells were regressed and disappeared. Erythr...
Faiz-ul Hassan1,*, Muhammad Sajjad Khan1, Muhammad Saif-ur-Rehman1, Syed Muhammad Raihan Dilshad2 and Muhammad Amjad Ali3
...Clevland, Noriker and Hanovarian) present in Pakistan were collected from Army Remount Stables while those of four Pakistani indigenous breeds (Morna, Saen, Kakka Bralanwala and Anmol) from local horse breeders of Punjab province. Genomic DNA was extracted from the blood samples and amplified by PCR using 40 RAPD primers. PCR products were separated on an agarose gel electrophoresis and scoring of bands was performed. Phylogenetic tree was constructed by UPGMA...
Asma Yousafzai1,*, Muhammad Luqman2, Nisar Ahmed1, Muneeza Arbab1,Muhammad Murad1, Naheed Sajjad3, Sara Naudhani1, Shakeela Daud1, Abdul Hameed Baloch4, Khushnaseeb5, Sana Zahoor6, Zahid Rauf7, Muhammad Mohsin Javed6 and Jamil Ahmad1
...enes for both breast and ovarian cancer and account for 15-20% of all the hereditary breast cancer cases. Present study was aimed at identifying mutation in BRCA1/2 in breast cancer patients in Balochistan. Blood samples of 100 subjects including 50 breast cancer cases and 50 normal subjects were used to determine genetic variants in BRCA1 and BRCA2. Nine variants were identified in BRCA1 and four were identified in BRCA2. In...
Abd El-Nasser Ahmed Mohammed1,2,*
...and oocyte quality after ovarian transplantation upon dietary supplementation of 1.0 and 2.0% of Nigella sativa (N. sativa) oil to female mice and secondly to explore if dietary N. sativa is effective in alleviation of hypothermia and hyperglycemia due to general anesthesia. Seventy-five albino female mice (21.74 ± 0.19) were distributed into three groups; control (G1; N=25; not receive N. sativa oil) and two N. sativa...
Ihsanullah*, Muhammad Subhan Qureshi, Sohail Akhtar and Syed Muhammad Suhail
...postpartum resumption of ovarian activities. Reproductive and productive records of crossbred dairy cows were analyzed at a large-scale state dairy farm from 1997 to 2013. Genetic groups consisted of HF 50.0%, HF 62.5%, HF 75.0% and HF 87.5%, having the respective levels of Holstein Friesian blood were studied in different seasons of the year. No significant differences were found between the groups with different exotic blood level in calving interval, age at...
Asim Faraz1*, Abdul Waheed1, Muhammad Mudasser Nazir2, Aneela Hameed3, Nasir Ali Tauqir4, Riaz Hussain Mirza1, Hafiz Muhammad Ishaq1, Rana Muhammad Bilal5
...luteum cysts, follicular ovarian cysts, delayed age at puberty, repeated estrous cycles, dystocia, abortions, dead fetuses and retention of the fetal membranes. OT administered exogenously or secreted endogenously, establishes in minutes the desired effects and metabolized to inactive products rapidly in the body. Irrespective of whether OT is ingested with milk or secreted into the milk, it is degraded readily by gut-enzymes so that it doesn’t reach the...

Paul Oludare Adetunji* and Safiriyu Idowu Ola 

...ubjected to Analysis of Covariance (ANCOVA) with body weight at puberty as a covariate while morphometry measurements were subjected to Spearman correlation analysis. The result showed that the plumage colour influenced (p<0.05) the onset of puberty in the cockerels with lower age at puberty, 155.29±2.41 days and 160.09±2.41 days recorded in brown and black cockerels, respectively compared to their barred counterpart, 167.23±2.41 days. ...

Amit Sharma1* and Pankaj Sood2

Sonographic Assessment of Ovarian Follicular Dynamics during Breeding and Non-Breeding Season in Gaddi Goats
... seasonal differences in ovarian follicular dynamics in Gaddi goats. Transrectal ultrasonographic ovarian examinations were carried out during a full estrous cycle in breeding (B; January-February, n=7) and 21 days in non-breeding (NB; May-June, n=11) seasons. Blood sampling on alternate days was done during both the seasons for serum progesterone (P4) estimations. Follicular growth was characterized by presence of three to ...

Paul Oludare Adetunji* and Safiriyu Idowu Ola

Comparative Productive Performance among three Plumage Varieties of Noiler Hens
...ubjected to Analysis of Covariance with body weight at puberty as a covariate, while morphometry measurements were subjected to Spearman correlation analysis. Plumage colour difference had a significant (P<0.05) effect on the onset of puberty as lower age at first egg were recorded in brown and black pullets compared to the barred pullets. Black hens had the best performance in most of the egg production indices with the values of 61.57±0.56g, 68.38&...
Muhammad Younis1, Muhammad Irfan-ur-Rehman Khan1*, Mustansar Abbas1Ali Murtaza1, Imran Mohsin2, Muhammad Shahzad3 and Muhammad Zahid Tahir1
...e study was to determine ovarian follicular development and steroid hormones concentrations during estrous cycle of Lohi sheep. Ovaries of nine Lohi ewes were monitored for two consecutive estrous cycles using B-mode trans-rectal ultrasonography during the breeding season (Sep-Nov 2018). Plasma progesterone and estradiol-17β concentrations were determined throughout the cycle using radioimmunoassay. The average length of the estrous cycle in Lohi sheep wa...
Md. Sabbir Ahamed1, Md. Rayhan Chowdhury1, Md. Atik Mas-ud1, Sujat Ahmed2, Md. Shahadat Hossain1 and Mohammad Nurul Matin1
 
 
...hest value of genotypic covariance (GCV) and phenotypic covariance (PCV) were found in FGPa (2312.334 and 2377.728) followed by UFGPa (18.472 and 74.639) and PH (437.76 and 438.34) suggesting that selection of this corresponding trait would be more effective. Identified low value of GCV than the PCV, suggesting environment may play influencial role on their phenotypic expression. Correlation coefficient values of yield attri...
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3
...owever, evidence of transovarian and transstadial transmission is a tick warrants an investigation into
the replication capacity of the virus in ticks. Transstadial occurs when virus remains with vector from
one life stage to the next and transovarian is transmission of virus from parent vector to its egg.
Objective: Investigation of LSDV replication capacity in ticks uses targeted detecti...

Jing Fu

...(CK20 Group) in mucinous ovarian tumors and their correlation with tumor pathological classification and prognostic outcome. One hundred and sixty cases of ovarian mucinous tumors diagnosed from January 2018 and January 2020 were selected. They were divided into four groups: mucinous cystadenocarcinoma group (age 62.78±7.92, n=53), borderline mucinous cystadenocarcinoma group (age 63.82±9.26, n=55), mucinous cy...

Mohamed Abd El-Fattah Abo-Farw1, Maged Ahmed Aboul-Omran1, Abdel-Khalek El-Sayed Abdel-Khalek2* 

...esumption of estrous and ovarian activity and decreased pregnancy rate, antioxidant status, thyroid and reproductive hormones and trace elements such as Zn and Se.

Keywords | Mastitis, Buffaloes, Pregnancy rate, Antioxidant, Trace elements. 

...

Aiman Al-Mufarji3, Abd El-Nasser Ahmed Mohammed3*, Rashid Al-Zeidi1, Haitham Al-Masruri2, Al-Hassan Mohammed4

...the peripartum period on ovarian follicular development, and blood and metabolic profiles with Moringa oleifera (M. oleifera) supplementation. Fifteen pregnant Naimi ewes (49.0 ± 1.85 kg body weight) were allocated to three groups: control and two M. oleifera treated groups (50.0 and 100.0 g M. oleifera /kg diet). Body weights, milk composition, blood cell counts (red and white blood cells), and metabolic profile (total protein and blood urea nitrogen) ...
Zeinab R. Aboezz1*, Ayman S. El-Habbaa1, Rania S. El-Mohamady2, Samia A. Elnagar2, Ehab M. El-Nahas1
...yptian cattle. Serum and ovarian samples were collected from cattle during the year of 2019. RT-PCR, viral isolation, sequencing, and phylogeny revealed that the primary causative agent was HoBi-like Pestivirus. Our HoBi-like Pestivirus strain, Egypt-020-1ncp, BVDV-Egypt-020-3ncp and BVDV-Egypt-020-2ncp, shared 99.6% homology with BVD3- B1-AU, 99.2% with BVD3- B5-3-MX and BVD3-G2-BR, 98% with BVD3- Hobi-like virus SA-2016-04 and 95% with BVD3- Italy-68-13ncp i...

Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6

...ive disorders and cystic ovarian disease (COD) is one of the important disease which cause major economic losses to farmers. COD affects fertility of animal which is important to reproduce young ones, that occurs due to negative impact factors on hypothalamus-pituitary stalkand normal function of the ovarywhich leads to alteration in follicular development, ovulation, reduced reproductive performance, unsuccessful ovulation, increased interval between parturit...

Jiacheng Du and Yuping Cao*

...ents with the polycystic ovarian syndrome (PCOS) who underwent Invitro fertilization-embryo transfer (IVF-ET). This study selected 200 patients with PCOS who took IVF-ET in our hospital from January 2016 to January 2020 as subjects. The study subjects were divided into group A (low-level group, n=90) and group B (high-level group, n=110) according to serum AMH levels; and group C (successful group, n= 88) and group D (failure group, n=112) according to whether...

Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1 

... fluid, granulosa cells, ovarian stroma and blood vessels), the fallopian tube (covering epithelium, sub mucosa and musculosa) and at the uterus (epithelium, endometrial glands, blood vessels and endometrial stroma). While in the acute cases, the positive BVDV antigen could not be observed in the primordial follicles, blood vessels or endometrial glands.

Keywords | Bovine Viral Diarrhea Virus, Immunohistochemistry, Ovaries, Oviducts, PI cow, Uteri &nbs...

Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Al-Hassan Mohammed4* 

...lease of oocyte from the ovarian follicle. Rodent oocyte maturation lasts 15-17hr. whereas that of ruminant oocyte lasts 24hr. Several factors affect on oocytes maturation either in vitro or in vivo and therefore affect development of the resulting embryos. Because of the significance of oocyte maturation stage on further embryonic development, this review aims to discuss the knowledge of oocyte maturation and its effect on further development of embryos in vi...

Abd El-Nasser Ahmed Mohammed*, Aiman Al Mufarji and Sultan Alawaid

...hese stages in mammalian ovarian follicles to prepare the oocyte for successful maturation, fertilization and further embryonic development. The changes enable the zygotes to overcome maternal zygotic transition stage and follow their developmental competence to fetus. The changes were affected by in vivo and in vitro molecules and factors of the organisms and the surrounding conditions, respectively. Because of the importance of follicular changes during grow...

N. Wachida1*, P. M. Dawuda2,1, I. U. Ate3,1, P. I. Rekwot4, 2 

...n follicle diameter, and ovarian hormones concentrations. Twelve (n=12) non-pregnant, Zebu cows with 2-3 successive calving record were utilized. Temperature humidity index (THI) determined and rectal temperatures of cows were monitored. Cows were synchronized for oestrus using PGF2α. Follicular studies were carried out. Serum oestradiol and luteinizing hormones were monitored by Enzyme-Linked Immunosorbent Assay (ELISA). Blood samples for serum were col...

Jiahua Peng1,2, Shiyao Hua3* and Qian Wu4

...eight, ovary weight) and ovarian tissue pathology changes were observed. The changes in content of testosterone (T), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were detected. The fasting blood glucose, serum insulin and insulin sensitive index changes were analyzed. The RT-PCR method was used to detect ovarian tissue TNF alpha and the level of IL-6 gene expression; Western blot was used to detect the cha...

Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1* 

...y five paired samples of ovarian tissue from buffaloes were obtained from slaughterhouse. Ovaries were collected and CLs were classified morphologically into three stages: early, mid and late stages. Moreover, the CLs were frozen at -80 °C for NO estimation in CLs homogenate, RNA isolation and gene expression. Blood samples were obtained for P4 serum levels estimation. Results showed a significant rise of TNFα mRNA during late stage of CL and increas...

Jianmin Liu1, Jiancheng Zhang2, Peijuan Wang3,* and Moslem Lari Najafi4

...on congrong tusi pill on ovarian artery blood flow and sex hormones in the adjuvant treatment of ovarian function decline. Ninety patients with ovarian function decline were randomly divided into 3 groups: new addition congrong tusi pill treatment group (n=30 cases), diethylstilbestrol treatment group (n=30 cases) and the control group (n=30 cases). A comparative analysis is made on sex ho...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
...ing, cryopreservation of ovarian tissues and germ cells and embryos, and genome resource banking for mammalian species. Such biotechnologies allow more offspring to be obtained from selected parents to increase milk and meat productivity, reduce the interval between generations, therapy of diseases and conservation of endangered mammalian species. Practically, current reproductive biotechnologies are species-specific because of differences in estrous cycle, se...

Aiman Al-Mufarji, Shaker Al-Suwaiegh, Abd El-Nasser Mohammed*

...onses, body weight gain, ovarian structures growth, blood and metabolic profiles of ewes and resulting lambs in subtropics. Fifteen ewes of body weight 49.1 ± 1.86 kg/head and 2.50 years old were allotted using complete random design to control and two M. oleifera groups (50.0 and 100.0 g/day). Relative humidity and ambient temperature were recorded and the temperature humidity index (THI) was calculated. Body weights of ewes and resulting lambs (kg) we...

Chenchen Liu1,2, Tong Wang1, Muhammad Khan3*, Xiaolin Cui2 and Yongming Li1,2*

...r effects of ALT against ovarian cancer remains unknown. Here, we report that ALT inhibits growth and induces apoptosis in parental and cisplatin-resistant ovarian cancer cells. Growth inhibitory effects of ALT are evident from CCK-8, Edu, and colonogenic assays. The molecular mechanism associated with induction of apoptosis includes ROS generation, G2/M phase arrest and inhibition of aerobic glycolysis. Inhibition of glycol...

Lei Jiang, Yi Huang, Runfeng Yang, Xiaohui Jiang* and Hao Yan

...cisplatin sensitivity of ovarian cancer cells by regulating the expression of miR-455-5p /PAX2. The expressions of circ_0009910, miR-455-5p and PAX2 mRNA in HOSEPiCs and ovarian cancer cells SKOV3 and SKOV3/DDP were detected by qRT-PCR. SKOV3/DDP cells were grouped according to the transfection of different genes. The sensitivity of SKOV3/DDP cells to cisplatin was detected by CCK8 assay, and the apoptosis of SKOV3/DDP cells...

Ibrahim Elmaghraby*, Abdel-Baset I. El-Mashad, Shawky A. Moustafa, Aziza A. Amin 

...rrent study investigated ovarian sex cord-stromal tumors in dromedary camels slaughtered at Egyptian slaughterhouses between January 2019 and February 2022. The study included 180 female single-humped camels in which all data on the animal’s age, location, and gross tumor lesions were documented, and positive ovaries were collected for further histological and immunohistochemical examination.Results: Interestingly, our results diagnosed 15 cases of sex c...

Trinil Susilawati1*, Aulia Puspita Anugra Yekti1, Amir Firdaus2, Dinda Ayu Damayanti2, Rizki Prafitri1, Nanang Febrianto1, Kuswati1, Achadiah Rachmawati1, Sri Wahjuningsih1, Nurul Isnaini1 

... at 4.35% and 8.34%, and ovarian hypo function at 0% and 4.16% in T1 and T2, respectively. In conclusion, Artificial Insemination using the double doses method did not significantly improve the pregnancy rate of dairy cows. 

...

Dajun Su1, Tao Deng2* and Mingshui Xie2

...eration and apoptosis of ovarian cancer cells by targeting miR-761. Ovarian cancer cells SKOV-3 and normal ovarian cells FTE187 were divided into groups according to the experimental procedures, and were transfected negative, respectively. The expressions of lncRNALOXL1-AS1 and miR-761 in ovarian cancer cells were measured by qRT-PCR (quantitative real-t...

Amna Mushtaq, Asia Bibi* and Nahid Kausar

...festations of polycystic ovarian syndrome (PCOS) have been linked with psychotic morbidity and infertility in the women of fertile age. Considering limited research data available about the psychological aspects in these patients, we conducted current study to assess psychotic distress and infertility in PCOS patients of southern Punjab, Pakistan. In this study 204 PCOS women and 150 controls were enrolled. Data of recruited patients was collected from differe...
Sri Rahayu*, Muhaimin Rifa’i and Widodo 
...crine variables regulate ovarian follicle growth from the preantral to the antral stages. Growth differentiation factor 9 (GDF9), which is secreted by oocytes, is a paracrine factor that plays a vital role in follicular development. We performed a computational biology analysis to determine the role of GFD9 in mammalian ovarian function. We constructed a three-dimensional model of GDF9 using I-TASSER and human GDF9 protein s...

Di Zhou1, Qingmeng Long1*, Rong Yang1, Xiaoshan Tan1, Jun Li1, Mingyan Tang1, Zhonghai Zhao3, Ye Ao2, Zhinan Zhou1 and Changxue Chen1

... collected from uterine, ovarian and fallopian tube tissues of goats with the same pedigree and analyzed the results using bioinformatics and qRT-PCR. We found 8120 differentially expressed genes (DEGs) in ovarian tissues (3205 up- and 4915 down-regulated), 5255 DEGs in the fallopian tubes (1317 up- and 3888 down-regulated) and 5180 DEGs in uterine tissue (2597 up- and 2583 down-regulated). The biological processes associate...

Sajida Batool1, Mubeen Akhtar1, Shafaat Yar Khan1,2*

...gn: justify;">Polycystic ovarian syndrome (PCOS) is among the common endocrine disorders in childbearing aged females. This study is designed to compare a few anthropometric, hematological, and biochemical parameters in PCOS patients with that of normal females including correlation between interleukin-3 and blood cell count. Patients with PCOS and normal healthy females (n=60) of same age group were included in this study following Rotterdam ESHRE/ASRM criter...

Cut Intan Novita1,2, Kartini Eriani3, Amalia Sutriana4, Tongku Nizwan Siregar5,6*, Ni Wayan Karja7

...mones are often added to ovarian vitrification media to prevent apoptosis of granulosa cell follicles. Bovine pituitary has been proven as an effective substitute for the functions of FSH and LH in some studies related to superovulation due to its high content of both hormones. Therefore, this study aimed to determine the effect of bovine pituitary extract (BPE) supplementaion to vitrification media. A total of 18 ovaries were usedwhich was divided into two gr...

Gokarna Gautam1*, Sajan Rokaya1, Bhuminand Devkota1, Saroj Sapkota2

... and to characterize the ovarian cyclicity status during the months of long day length. Data regarding seasonality pattern of breeding and calving of Murrah cross bred buffaloes (n=400) from two locations of subtropical region were collected. In a subset of buffaloes (n=104), the ovarian cyclicity status during late spring (May to June) was determined through transrectal ultrasonography and measurement of blood progesterone ...

Muhammad Yusuf1*, Abd Latief Toleng1, Sahiruddin1, Masturi1, Hasrin1, Musdalifa Mansur2, Hendrikus Fatem3, Johan Koibur4

...udy was to determine the ovarian responses of dairy heifers after treated with estrous synchronization using Heasynch and Select Synch protocols. The study was conducted in Maiwa Breeding Centre (MBC) Farm of Hasanuddin University. A total of 18 dairy Holstein Friesian heifers were used in the present study. The heifers were then divided into two groups based on different treatments of estrous synchronization. All heifers were examined for

Gokarna Gautam1*, Hari Adhikari1, Bhuminand Devkota1, Subir Singh2

... was not affected by the ovarian cyclicity status at the initiation of the protocol. In conclusion, incorporation of CIDR into Ovsynch protocol improved the reproductive performance of anestrous buffaloes during low breeding season. 
 
Keywords | Buffalo, Low breeding season, CIDR, Ovsynch, Pregnancy, Corpus luteum
...

Dio Fico Felsidan Diatmono1, Seraphina Kumala1, Pradita Iustitia Sitaresmi2, Stefani Winda Paramita1, Megawati Andi1, Yustina Yuni Suranindyah3, Diah Tri Widayati1*

Farhatul-Ain Arshad1, Asia Bibi1*, Amna Mushtaq1, Rubaida Mehmood2 and Nahid Kausar1

...justify;">The polycystic ovarian syndrome (PCOS) is an endocrine disease in reproductively aged women and is charactered bydyslipidemia, hyper-androgenism, acne, hirsutism, menstrual irregularities, and ovarian dysfunction resulting in infertility. A balanced body mass index, physical fitness, biochemical and endocrine ratios play vital role in proper functioning of reproductive system and guard against PCOS development. Thi...

Gökhan Ulukan1*, Zeynep Pekcan2, Zülfükar Kadir Sarıtas3, Ilker Etikan4, Serkan Sayıner5, Feride Zabitler6 and Fatma Eser Özgencil1

... administered inside the ovarian bursa 10 min before ovary removal in Groups (G) 1 and G2 and linear to the incision line 10 min before entering the abdomen in G1. G3 was the control group. The intergroup comparison of pain mediators and GCPS-SF scores showed no significant difference between the GCPS-SF scores at postop0, postop2, postop4, postop8 and postop24 h and the cortisol, TNF-α, IL1-β and NO levels determined at the same timepoints. TNF-&al...

Hafizuddin1, Sri Wahyuni2, Tongku Nizwan Siregar1*, Amalia Sutriana3, Ririn Triyuliani4, Siti Hanyyah Herviani4, Muhammad Farhan Syuhada4, Nur Salimah4, Muharni Mardiah Eriswandi4, Ratu Permata Ayu4, Putroe Hayatun Nufus4

...dy aims to determine the ovarian and hormonal responses of local rabbits after superovulation induction with BPE. The sample comprised of 1 local male rabbit and 9 local female rabbits with a history of giving birth and weighing between 1.8-2.2 kg. The animals were adapted for 30 days in separate cages and provided with feed and water ad libitum before treatment. The rabbits were divided into 3 treatment groups (n=3), the first group as a control group (R1) wa...

Advances in Animal and Veterinary Sciences

July

Vol. 12, Iss. 7, pp. 1206-1409

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