Haq Aman Ullah1*, Aneela Zameer Durrani2, Muhammad Ijaz2 and Aqeel Javeed3
Antibacterial Activity by Chlorogenic Acid Isolated through Resin from Apricot (Prunus Armeniaca L.)
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
... the fast protein liquid chromatography. The lactoferrin was further characterized through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Disc diffusion method was applied for the evaluation of antibacterial activity of lactoferrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lactoferrin...
Muhammad Aslam Farooqi1,*, Mansoor-ul-Hasan2, Sohail Akhtar1, Muhammad Arshad2, Muhammad Naveed Aslam3 and Muhammad Rafay4
Ayesha Aihetasham1,*, Muhammad Saeed Akhtar1, Maryam Umer1, Khalid Zamir Rasib2 and Muhammad Imran Din3
...ion of plant extracts by chromatography-mass spectrometry (GC-MS) revealed five different compounds in F. vulgare: Piperidine, 3-isopropyl, Bicyclo[2.2.1]heptan-2-one, 1,3,3-trimethyl, Benzaldehyde, 4-methoxy, Estragole, 11-Octadecenoic acid, methyl ester and 9-Octadecenoic acid ethyl ester. Whereas nine different compounds were identified in extracts of O. basilicum. These were: 1-Isopropyl-2, 2-dimethylpropylideneamine, Camphor, Naphthalene, Th...
Amtul Jamil Sami1,*, Madeeha Khalid1, Rehman Shehzad1, Sana Mughal1 and A.R. Shakoori2
...rified by gel filtration chromatography using Sephadex-G100 and ion exchange chromatography on DEAE- Sepharose, employing a linear salt gradient. SDS-PAGE was used to monitor the purification steps, and protein band was located by immunoblotting technique. Bubaline placental lactogen was purified to homogeneity and showed a molecular weight of about 30 kD. Purified placental lactogen was administered to male albino mice for ...
Zafar Abbas*, Ijaz Ahmad**, Adnan Shakeel**, Muhammad Abdullah***, Muhammad Islam**, Sadiq Muhammad*, Ghulam Murtaza* and Mushtaq Ahmad**
EFFECT OF PHOSPHORUS FERTILIZER AND WATER STRESS ON PROTEIN AND PHENOLIC CONTENTS IN COTTON (GOSSYPIUM HIRSUTUM L.)
...hl method and thin layer chromatography (TLC) were used for the quantitative and qualitative analysis of total protein and phenolic compounds, respectively. Proteins were greatly affected by fertilizer treatment and water stress, but phenolic compounds remained unchanged upon fertilizer treatment. However, they were greatly affected by irrigation and water -1 stress. Crop treated with 100 kg ha P O under water stress maintained high 2 5 protein content as comp...
Anum Rafique, Sajid Abdullah, Khalid Abbas, Huma Naz* and Wardah Hassan
...ion. Ion-exchange column chromatography was used to purify enzyme for further partial characterization against different range of temperature and pH. As a result of ion exchange chromatography enzyme from kidney of control and Pb+Cr treated fish had highest activity as 508.33 and 427 UmL-1 while highest specific activity was 1105.06 and 1055.55 Umg-1 with 1.98 and 2.70 fold purification, respectively. R...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
... purified using affinity chromatography. SUMO tag was removed from SUMO-IFNα2-Tα1 by SUMO protease and recombinant IFNα2-Tα1 was collected in flow through by affinity chromatography. The MW (~23 kDa) of IFNα2-Tα1 was determined by 12 % SDS-PAGE and its integrity was confirmed by Immuno blot analysis using anti-interferon α-2 and anti-thymosin α-1 antibodies. Purified IFNα...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...s was done by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC-UV) to get the indication of the identity of the compounds responsible for antimicrobial activity and biofilm dispersion. Overall the results indicated that natural extracts from these Streptomyces strains and their active components can be exploited for the preparation of anti-caries products like mouth washes and tooth pas...
Jia Chen*, Yuanxi Deng and Hui Xu
...hange and gel filtration chromatography, and the MALDI-TOF/TOF-MS search on the Internet. The results indicate that the protein was diamine- N-acetyltransferase.
...
Soumble Zulfiqar1, Khuram Shehzad1, Sana Tahir1, Khalid A. Al-Ghanim2 and Abdul Rauf Shakoori1,2,3,*
...neity by nickel affinity chromatography. Enzyme assays of CueO protein with phenolic substrates revealed its laccase activity. The kinetic studies showed Km value of 0.2µM, kKcat 0.68 S-1 and Kcat/km 1.2S-1 µM-1 for 2,6-Dimethoxyphenol (DMP) and Km value of 0.25mM, Kcat 300 S-1 and Kcat/Km=1200S-1mM-1 for Syringaldazine (SGZ). Regulation of cueO in response to various concentr...
Dilara Abbas Bukhari1, Abdul Rehman2*
Isolation of bioactive compounds from exudate of edible fungus, Pleurotus ostreatus
...l techniques, thin layer chromatography, high performance liquid chromatography and gas chromatography/mass spectrometry, were employed to study fungal exudate. A total of fifteen different metabolites were detected in the sample by GC/MS. The detected metabolites could be classified into chemical groups of 2 esters, 4 alcohols, 3 benzoic acids, ketone, aldehyde, phenyl and amide groups co...
Sahar Shibli1,2*, Farzana Siddique1, Saeeda Raza2, Zaheer Ahsan3 and Irum Raza4
...ivars through gas liquid chromatography revealed six fatty acids namely palmitic acid, oleic acid, linoleic acid, arachidic acid, eicosenoic acid and behenic acid. The peanut cultivars Bard-479 and Local-334 were more suitable for oil extraction and peanut butter development because of their high oleic acid to linoleic acid ratio (2.3-2.4). Bard-92 was less preferable cultivar for product development owing to its high linoleic acid (42.56%) and low O/L ratio (...
Aisha Khalid1, Muhammad Tayyab1,*, Abdual Rauf Shakoori2, Abu Saeed Hashmi1, Tahir Yaqub3, Ali Raza Awan1, Muhammad Wasim1, Sehrish Firyal1, Zaheer Hussain4 and Munir Ahmad5
...ified by affinity column chromatography. The characterization studies of purified protein demonstrated the optimal enzyme activity at 90°C and pH 4.8. The presence of cobalt enhanced the cellulase activity and 2.5 mM cobalt was recorded the optimal concentration for the maximal cellulase activity. SDS-PAGE analysis confirmed the molecular weight of recombinant protein as 39 kDa. The protein was found thermostable which retained more than 70% re...
Doğukan Ölmez1 and Gonca Alak2,*
...h performance thin layer chromatography (HPTLC) method. As a result of the analyses, it was observed that there were 9 protein bands in the muscle and 7 protein bands in the visceral organs, and the volume increases were observed in protein amounts in the hydrolysates depending on time. In addition, as the lipid profile, cholesterol ester (CE), triacylglycerol (TAG), free fatty acids (FFA), cholesterol (CHOL), monoacylglycerol (MAG), diacylglycerol (DAG) and p...
Muhammad Shahid Nadeem*, Maryam A. Al-Ghamdi and Jalaluddin Azam Khan
...lum based anion-exchange chromatography. On SDS-PAGE, the enzyme exhibited a molecular weight of about 36 KDa. Its specific activity was 1650 U per mg of protein with about 5% glutaminase activity and no activity against D-asparagine. Optimal enzyme activity was found at 75°C and pH 9. The KM value of 5.9mM was found for L-asparagine. In silico studies have shown that the enzyme exists as a homotetramer. Molecular docking studies have rev...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...ge on Q-Sepharose column chromatography. Polygalacturonase was recognised as a protein with 47kDa molecular weight by SDS-PAGE. The optimum pH of purified polygalacturonase activity was found to be 4.5 and stable within pH range 3.5-5.5. Temperature dependent studies revealed temperature optimum of enzyme to be 40°C and stable up to 60°C. Among substrates, polygalacturonic acid was established as the best substrate for polygalacturonase showing its spe...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...ultra-performance liquid chromatography (UPLC) and Bruker micOTOF OllQ TOF mass spectrometry, respectively. The full amino-acid sequence of the monomericpeptide was analyzed by sequential Edman degradation using a protein/peptide sequencer. The antibacterial activity of the protein monomer in antlerplate was studied by disc diffusion method, and the antibacterial ring diameter of each antibacterial agent was measured to determine its antibacterial ability. The...
Yating Cheng1, Wenlong Shi1, Xue Xiao2, Qirong Zhang2, Qihao Zhang1, Zhijian Su1, Qi Xiang1,2* and Yadong Huang1,2
...nd ascorbic acid. Liquid chromatography coupled with tandem mass spectrometry was used to monitor the hydroxylation of proline. The results showed that rhP4H specifically hydroxylated proline residues only in the Gly-X-Y structure but did not react with free proline. We hypothesize that rhP4H can be engineered to control structural properties of recombinant collagen by selecting a certain degree of hydroxylation of proline residues, which should contribute to ...
Haq Aman Ullah1,*, Aneela Zameer Durrani2, Muhammad Ijaz2, Aqeel Javeed3, Muqadder Shah1 and Ikramul Haq1
... high performance liquid chromatography (HPLC), somatic cell count (SCC) and total viable count (TVC). Blood samples were analyzed for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels. Aflatoxin M1 (AFM1) was detected in all milk samples of the Group B, C, and D in concentration higher than 0.05 ppb. The AFB1 was excreted in milk as metabolite AFM1 @ 1.35-1.59%. Udder health and milk quality deteriorated as...
Mahnoor Pervez and Farkhanda Manzoor*
... high performance liquid chromatography analysis. Pollen and nectar were collected from 2016 to 2017 from different agricultural areas of Pakistan during a field survey. Pollen was collected by installing traps and nectar was collected through capillary method allied with centrifugation. The HPLC technique was used for fast and simple detection of pesticide residues of commonly used pesticides (imidacloprid, deltamethrin, chlorpyrifos, fipronil, thiamethoxam, ...
Sumaira Abbas1, Muhammad Sultan Haider2,*, Fatima Kafayet1, Sana Ashraf2, Atifa Masood3 and Moazma Batool4
...determined by thin layer chromatography (TLC) and found satisfactory. By mixing fish meal, sunflower meal and rice polish four treatment diets, T1, T2, T3, were prepared and citrus peel powder was added @ 200g, 400g and 600g/ 1000g, respectively. While fourth one, control diet (To) was without citrus peels. These is nitrogenous diets (30% protein) were offered to Carassius auratus juvenile having body weight 2...
Honghua Wang1,2, Summia Perveen1,2, Shucheng Shao1,2, Kun Wang1,2 and Fei Yin1,2,*
...t wall, a shotgun-liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique was applied and all identified proteins were categorised using a bioinformatics approach. The results showed that 3,472 proteins were identified with a theoretical molecular weight of 11.53–610.90 kDa and an isoelectric point of 4.13–11.77. A total of 63.07% or 50.09% proteins were annotated by Kyoto Encyclopaedia of Genes and Genomes (KEGG) or Gene Ontology (GO) ...
Muhammad Faheem Akbar1,*, Muhammad Abdul Haq2, Imtiaz Ahmad1, Vasileva Viliana3 and Amjad Sultan1
... high performance liquid chromatography. First order degradation kinetics was fitted on this data and degradation rate constants and half-life were calculated. Conventional pesticides were found to be more persistent in the crop (Average half-life: 2.94, 4.06 and 2.74 days for imidacloprid, endosulfan and profenofos respectively) than bio pesticides (Average half-life: 3.87 and 1.81 days for spinosad and biosal respectively). The crops treated with bio pestici...
Amna Shoaib*, Zoia Arshad Awan and Naureen Akhtar
...production by thin-layer chromatography (TLC). A polyphasic strategy as adopted for the current study is a reliable and reproducible means to differentiate A. minisclerotigenes from A. flavus, indeed essential in interpretations of taxonomic and nomenclature of A. flavus group that may allow prior diagnosis and selection of effectual antifungal agents.
...
Boxin Dou, Ying Liu*, Yumeng Liu, Lili Fan, Yongqiang Ma, and Yanguo Shi
...ltration, size exclusion chromatography and RP-HPLC. Using consecutive chromatographic techniques successively, the acidic hydrolysates of RB proteins were fractionated and the new ACE inhibitory triplet was isolated and identified. The amino acid sequence of the ACEI was identified as Ile-Thr-Leu or Leu-Thr-Ile. In vitro, ACE inhibition assays showed that the IC50 value of the peptide was 0.0118 mg.ml-1. The inhibitory ...
Yan-Guo Han, Jia-Yuan Wu, Ri-Su Na, Wei-Jiang Si, Yu-Qin Han, Yan Zeng, Yong-Ju Zhao and Yong-Fu Huang*
... were detected using gas chromatography. Immunisation of oral and plasmid-injected KISS1 DNA vaccines induced strong antibody response, significantly suppressed testosterone secretion and reduced scrotal circumference and testis weight. It also significantly reduced the concentrations of 4-methyloctanoic acid and 4-methylnonanoic acid in adipose tissue. These findings indicated that immunisation of oral and plasmid-injected KISS1 DNA vaccines can...
Raza Ullah Khan1*, Ahmad Khan1, Mohammad Zameer Khan1, Fayyaz Hussain1, Zafar Islam2 and Muhammad Asad Hameed2
... High Performance Liquid chromatography, Fourier transform infrared spectroscopy and atomic absorption spectrophotometer. The analysis showed the presence of hydrophobic and hydrophilic sites. The Fourier infrared spectroscopy analysis showed spectrum bends at different wavenumbers while elemental analysis show existence of different macro and micro nutrients which can be used to supplement the plant needs for nutrients by incorporation of humic substances alo...
Hesham Saeed1*, Manal Abdel-Fattah1, Ahmad Eldoksh1, Farid S. Ataya2 and Manal Shalaby3
... Ni-NTA Agarose affinity chromatography and the purity was judged by SDS-PAGE. Moreover, the effect of the recombinant IFNα of the viability of cancer cell line was assessed by MTT assay. Morphological study showed that C. dromedarius IFNα protein inhibited cell survival of MDA-MB-231 triple negative breast cancer cells.
...
Bushra Baig and Saeeda Yousaf*
Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1
... callus using thin layer chromatography (TLC). The profiling of ethanol extracts of callus of two varieties O. aristatus were carried out using solvent system toluene-ethyl acetate- formic acid-water (3:3:1:0.2). The results of monitoring TLC suggest that callus obtained from Schenk and Hildebrandt (SH) media can proceed to the suspension culture stage because it showed brighter fluorescence spot than other callus and plant extracts, especially for rosma...
Enas K. Abo-Elmagd,Kouka S. Abd El-Wahab,Azza H. El- Salakawy
...e of Lateral flow immuno-chromatography assay (LFICA) as a rapid test for detection of RV antigen in stool specimens collected from Egyptian infants and young children with ELISA, and nested reverse transcription polymerase chain reaction (RT-PCR). Furthermore, to study the frequency of RV infection in Egyptian infants and young children during the summer season 2012 and the effect of certain risk factors including age and gender on the extent and impact of RV...
Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
...c acid (Ni-NTA) affinity chromatography. The bioactivity of AGR2 was tested by cell proliferation and wound healing assays, while the auto-fluorescent property of DsRed was confirmed by fluorescence microscopy and immunofluorescence. The potential application of his-AGR2-DsRed was demonstrated by its internalization in fibroblasts. Therefore, the purified recombinant fluorescent protein would be a very useful tool for further investigation of AGR2’s extr...
Nasir Mahmood Cheema1*, Ghulam Shabbir2 and Nazakat Nawaz3
...action apparatus and gas chromatography. Results revealed significant differences among oil quality components among castor cultivars and differ significantly (p<0.05) from the view point of oil content, linoleic acid, stearic acid, palmitic acid and protein but indicated no difference significantly (p>0.05) in oleic acid and ricin oleic acid. Among cultivars, DS-30 showed maximum oil content (49.356 %) and ricin oleic acid (87.978%). The most important ...
Suhu Duan, Zaiqiang Li, Zhenzhen Fan, Mengran Qin, Xiaoxue Yu* and Liuan Li*
...k were determined by gas chromatography with 24 eggs randomly selected from each group on the 10th, 25th and 40th day after start of the treatment. The results showed that on 10th, 25th and 40th day, the contents of α-linolenic acid (ALA, C18:3n3) and docosahexaenoic acid (DHA, C22:6n3) were significantly increased in yolk of 6% and 15% seed group (P < 0.05). On 25th d...
Ali Ikhwan1*, Dian Indratmi1, Faridlotul Hasanah1, Manar Fayiz Mousa Atoum2,3 and Irum Iqrar4,5
Prima Mei Widiyanti1*, Mirnawati B Sudarwanto2, Etih Sudarnika2, Raphaella Widiastuti1
... high performance liquid chromatography (HPLC) method. This study showed that no heating (control), heating at 72 oC for 15 seconds (high temperature short time pasteurization), and 89 oC for 1 second (higher heat shorter time pasteurization) did not affect on enrofloxacin antibiotic residue concentration, however heating at 121 oC for 15 minutes (sterilization) affected the antibiotic enrofloxacin. The stability of enrofloxacin antibiotic could result problem...
Lin Huang1, Ling Mai2, Keyan Zhong1 and Xinjun Chen3*
...y Ni-IDA resins affinity chromatography, and were finally confirmed by SDS-PAGE and Western blot. The results showed that SeFABP, a stable intracellular protein without signal peptide and transmembrane region, was composed of 130 amino acids (AA). It contained 10 phosphorylation sites and 7 post-translational modification sites. Both methods predicted that SeFABP contained 5 linear epitopes, and were highly coincident, indicating that SeFABP had good immunogen...
Riaz Shah1*, Shaikha Hilal Al Ismaili2, Sheikha S. Al-Siaby1, Amal Mohammed Al Nasiri2, Thuraiya Hafidh Al Maskari2, Jamal AlSabahi3 and Huda Al-Ruqaishi3
Heba Mohamed Shaheen1, Ali Meawad Ahmed1*, Hosny Abdelltief Abdelrahman1, Rania Helmy Abohatab Abdou2, Aya Salama Mohamed Kamel1
Nahla Hamada Magd Khalil1*, Ihab Mahmoud Helal2, El-Desoky Hassan Ibrahim Dorrah1, Soad Ahmed Soliman Ismail3
...de residues (OCP) by gas chromatography. The results revealed presence of different types of OCP residues (Dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethane (DDD), chlorodiphenyldichloroethylene (DDE), Alderin, Dieldrin, Heptachlor, Heptachlor epoxide, Alpha hexachlorocyclohexanes (αHCH), Gamma hexachlorocyclohexanes (γHCH), Endosulfan and Gamma chlordane (γ-chlordane) in the examined fish species at different concentratio...
Nabila Khan1, Imran Ahmad2 and Muhammad Bilal Sadiq1*
...ctrometer (FTIR) and gas chromatography mass spectrometer (GCMS). The extraction conditions were optimized by using independent extraction parameters; sample to solvent ratio (1:20-1:40 w/v), solvent concentrations (20-80%, v/v) and time (10-30 min), whereas, TPC and DPPH were used as response variables. At optimized extraction conditions (40 ml/g, 22.5 min and 80% of ethanol), the experimental values for TPC and DPPH inhibition were 110.17 ± 3.44 mg of...
Mubasshir Sohail*, Qadeer Ahmed Soomro, Raza Muhammad, Muhammad Usman Asif and Imran Rauf
...phoresis. Gel filtration chromatography showed the presence of multiple forms of enzyme activities with different molecular weights. SDS-PAGE showed the presence of endoglucanase activities with molecular weight of 57 kDa....
Shuhuan Li1*, Yongheng Bo2, Youzhi Li3 and Xiuzhen Yang2
... high performance liquid chromatography (HPLC), a simple but potent method was used to quantify atropine residues in beef. The results indicated that the method could measure atropine residues accurately, with the average recoveries between 82.37 and 88.31 % when the additional concentration ranged from 0.5 to 5.0 mg/kg, and a detection limit of 0.25 mg/kg, providing an effective and reliable method for the detection of atropine residues in beef.
...
Gamalat Y. Osman1, Elham M. Hassan1*, Tarek A. Salem2, Azza H. Mohamed1
... high-performance liquid chromatography (HPLC) technique. The main recorded constituents were rutin, P-coumaric, hydroxyl-benzoic acid, ferulic acid, and sinapic acid. Results of the present study indicated that FWE (0.5 ml/mouse for 10 successive days) exhibited a significant reduction (P<0.05) in worm burden. A scan electron microscope examination of male worms showed tegument corrosions with reduced spines density and abnormal spine tubular shape. Treatm...
Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby*
...(High-performance liquid chromatography). In this research, we used polymerase chain reaction (PCR) for the detection of the mcr-1 gene, and phylogeny was performed on three isolates to detect mcr-1 gene mutations and relationships. The percentage of isolated E. coli was 25.5%. All isolates showed resistance to colistin in disc-diffusion assay, while in MIC (minimum inhibitory concentration) method 68.8% exhibited resistance. Colistin was recorded as 33.7% in ...
Reyad R. Shawish, Reda E. Hamed*, Zakaria H. Elbayoumi
... Egypt. By using the gas chromatography–mass spectrometry (GC–MS) technology, all collected samples were examined for the presence of certain polycyclic aromatic hydrocarbons (PAHs), and comparison of their contents with the recommended standard limits. Regarding the obtained findings, kabab recorded the highest concentrations of PAHs and PAH4 (the sum of four different polycyclic aromatic hydrocarbons, named benzo[a]anthracene, chrysene, benzo[b]f...
Mostafa El-Sebelgy1*, Hanafy Madbouly2, Sabry Tamam2, Nagwa Ata1, Kawther Zaher1
...as analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) using a technique called untargeted (label-free) shotgun proteomic analysis. The resultant peptides were used along with RefSeq proteins and primers for multiple sequence alignment. Multiple extrinsic proteins [NS1, NS3 and VP3 of African Horse Sickness Virus (AHSV) and NS3 of Equine Encephalosis Virus (EEV)] were detected and at the same time, integration events were examined in ...
Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction
Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3
...tered to affinity column chromatography [Cyanogen Bromide-Sepharose 4B (CNBr-Sepharose 4B)] (Sigma Chemical Co.), obtain the fraction. In brief, IgG of cows S. aureus positive sera were purified by precipitation with 50% ammonium sulphate followed by purification with Protein A Sepharose gel. The separated IgG was dialyzed for 3 days against a 0.1 M NaHCO3 buffer pH 8.3 with 0.5 M sodium chloride and 0.02 percent NaN3 before being linked to CNBr-Sepharose 4B s...
Zahra Naz1,2, Fouzia Ismat1, Muhammad Saleem2, Mazhar Iqbal1, Aamir Shehzad1 and Moazur Rahman1,2*
...le form through affinity chromatography without compromising the structural integrity of the protein. Using the purified M protein as a diagnostic antigen, an indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of anti-NDV antibodies in multiple serum samples collected from different poultry farms of district Faisalabad, Pakistan. The data presented here reveal that the recombinant detergent-solubilized M protein is an activ...
Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3
... High Performance Liquid chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyzed. Aspartate aminotransferase, alanine aminotransferase, urea, and creatinine serum level were also estimated. From our findings, we concluded that the usage of a half-dose combi...
Mansha Tahir1, Abdul Rauf2,3, Syed Ayaz Kazmi2,3*, Nuzhat Shafi2, Muhammad Hasnain Ali Gillani3, Sundus Khawaja4, Syeda Maria Fiaz Bukhari4, Tehniat Summer4 and Muhammad Majid Bashir2
Fahrauk Faramayuda*, Soraya Riyanti and Sitty Mahanadhiandinie
...The result of thin-layer chromatography (TLC) profiles extract ethanol, n-hexane fraction and ethyl acetate fraction showed the presence of flavonoids, polyphenols, and monoterpene-sesquiterpenes. Ethyl acetate extract of white tea leaves has very strong antioxidant activity....
Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5
... high-performance liquid chromatography (HPLC) technique. Skin histopathology and immunohistochemistry of collagen were performed. Results: Ivermectin was detected in 36 samples, out of them 15 contained high ivermectin levels (100 ppb). Chlorpyrifos, piperonyl butoxide, and acetamiprid were below the limit of quantification in 3 samples only. Histopathology of tick-infested skin revealed severe multifocal eosinophilic dermatitis and inflammation of subcutaneo...
Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2
Mustafa Erkan Özgür1*, Selim Erdogan2, Songül Aydemir3 and Hatice Yumusakbas2
...ed in fresh semen by gas chromatography (GC) and investigated the relationships with the sperm cell velocities. According to our results, total levels of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for Salmo trutta fario occurred in higher quantities than Oncorhynchus mykiss but the quantities of total polyunsaturated fatty acids (PUFA) were less. Palmitic and stearic acid for SFA, oleic and nervonic acids for MUFA and linolenic, arachid...
Oluwatoyin Adenike Fabiyi1*, Mariam Temitope Baker2 and Gabriel Ademola Olatunji2
... was subjected to column chromatography (silica gel 100-120 mesh grade), which afforded fractions that were analysed with GCMS and FTIR for constituent identification. The result shows octanoic acid; hexanoic acid methyl ester; ethyl octanoate; 9, 12-octadecadienoic acid methyl ester; dodecanoic acid; octadecanoic acid methyl ester; decanoic acid; octadecanoic acid ethyl ester and tetradecanoic acid as the major components while the infra red spectral diagnost...
Muhammad Shahid Nadeem*, Jalaluddin Azam Khan and Firoz Anwar
...rified by anion exchange chromatography, it displayed a 45kDa band on SDS-PAGE, with 58.1% final recovery, 15.3 fold purification and 139 U/mg specific activity. Maximum enzyme activity was found at pH 8 and above 90C, its KM and Vmax values were found 25µM L-alanine and 149 U/min/mg respectively. In silico studies have shown that the enzyme was found in a monomer structure. Molecular docking studies with potential molecules involved in the reaction cata...
Aqsa Javaid, Masoom Yasinzai* and Khunsa Saeed Kiyani
...ation and gel filtration chromatography using Sephadex G-50). The molecular weight of extracellular phytase from B. subtilis KT004404 estimated by SDS-PAGE was 30 kDa. The Km and Vmax of B. subtilis KT004404 phytase were 0.175 mM and 250 U/mL (phytase activity) respectively. The optimum temperature for partially purified extracellular phytase was 40°C, and the optimum pH range for extracellular phytase activity was pH 6.5-7.0. The extracellular enzyme acti...
A.M. El-Baha1, A. A. El-Sherbiny2†, M. Z. M. Salem1, N. Shaarawy3 and N. H. Mohamed3
...ils were analyzed by gas chromatography-mass spectrometry (GC-MS). Isopulegol (53.68%), citronellol
(15.26%) and isopulegol acetate (15.25%) were found to be major constituents of C. citriodora, while eucalyptol
(55.36%) and α-pinene (14.87%) were the major components of E. camaldulensis leaves EO. Because of the high
nematicidal performance of studied EOs, further trials are required to investigate their efficacy in c...
S. Anwar1, K. Wieczorek1 and E. Inselsbacher2 †
O. A. Fabiyi
.../div>
subjected to chromatography on a silica gel packed column. The nematicidal activity of the fractions were
evaluated in the laboratory using a 4x4x3 factorial experiment, while the crude extracts were tested on
Meloidogyne incognita infecting Abelmoschus esculentus in the field. Three hundred juveniles were used for the
assessment. Acetone extract coded DNLO/Me2CO caused a significant increase in plant height, num...
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...cid composition with gas chromatography and lipid peroxidation TBARS (Thiobarbituric acid reactive substances) content were used for oxidative stress evaluation. Frozen-thawed results showed significant (p < 0.05) enhancement in all sperm parameters with 10 ng/ml of DHA supplementation. DHA improved (p < 0.05) SOD levels in all supplemented groups compared with the control group. Level of TBARS was higher (p < 0.05) at 15 ng/ml as compared to other DH...
O. A. Fabiyi, G. A. Olatunji and O. Atolani
...subjected to open column chromatography on silica gel (100-120 mesh grades) using glass column. The chromatographic fractions were tested in vitro along with their crudes and carbofuran on Meloidogyne incognita
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5
...mbrane integrity. Liquid chromatography-mass spectrometry (LCMS/MS) analysis was used to assess the confirmation profile protein of seminal plasma. The conception rate from artificial insemination was used to determine the fertility rate (AI). This investigation revealed that the quality of the sperms from Bali-polled bulls and Bali-horned bulls did not substantially change after thawing (p > 0.05). However, sperm acrosome integrity was considerably (p <...
Maryam Beheshti1, Mohammad Reza Taheriyan2*, Sikander Shahzad3, Memoona Siddique4, He Tianwei5, Muhammad Farooq5*, Huzaifa Kashif6 and Naila Ilyas7*
...and measured using a gas chromatography-mass spectrometer. Rats were separated into 5 groups of 8 and given treatments of hemp, palm, and soybean totaling 1.5% of their body weight in order to study the effects of various treatments on their blood parameters. Blood tests were used to investigate these parameters. Based on the results, all three oils-hemp, soybean, and palm-had the same refractive index (1.46) and showed no statistically significant different (...
Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2*
...e. Methods: Rapid immune-chromatography and direct fluorescent antibody test (DFAT) were applied to detect RABV antigens in the brain of rabid dog. Relevant reads from the Ion S5 sequencing system were assembled into a draft genome. The draft genome was analyzed, re-assembled, and compared to representative RABV genomes from Africa and the Middle East region. Results: The complete genome of 5EG-QH19 strain extends for 11919 nt including the standard RABV prote...
Burarah Arooj1, Zeeshan Mutahir1, Moazzam Ali1, Mohsina Akhter2, Malik Siddique Mahmood1, Arslan Hamid3 and Mahjabeen Saleem1*
...tration and ion exchange chromatography were used to purify the recombinant 52 kDa protein. Purified chitinase showed optimum activity at 60°C and pH 5.5 with colloidal chitin breakdown. Enzyme showed stable residual activity within pH range of 4.5–6.5 and >70% thermal stability upto 60°C for 2.5 h. The activity of chitinase increased in the presence of Mn+2, SDS, and methanol. Chitinase has Km and Vmax values of 2.287 mg/ml and 6.784 µM...
Saad Tahir, Nadeem Ahmed*, Mohsin Ahmad Khan, Muhammad Akram, Rabia Abbas and Kausar Malik
...otein. Further, affinity chromatography was carried out for the purification of the HSA-SK fusion protein. SDS-PAGE, western blot, and RP-HPLC analysis were also conducted, which confirmed the 98% purity of the product with 40% yeild having a molecular weight of almost 111kDa. The purified fusion protein (HSA-SK) was also verified via western blotting by using anti-streptokinase antibodies. Clot lysis assay was used to estimate the biological activity of HSA-S...
KHADIJA SUMMIA*1, ROHEELA YASMEEN*2, ALEEM UN NISA3, SAMIA DJEFFAL4 & TAHIRA JABEEN1
...s level using thin layer chromatography (TLC) method. The results showed that out of 50 samples, 20 (40 %) were fit as no level of aflatoxins detected in these samples. However, 30 (60 %) were positive for aflatoxins. 15(50 %) samples were highly contaminated and unfit. However, the other 15 (50 %) samples were within the tolerance range of aflatoxins. Aflatoxin B1 was detected in all 30 samples and aflatoxin B2 was noticed in only 10 samples. However, Aflatox...
Muhammad Syarif Djaya1, Osfar Sjofjan2, Hartutik Hartutik2, Irfan Hadji Djunaidi2
Fatemeh Beheshti1, Maliheh Safavi2, Mohammad Reza Akbari Eidgahi1,3, Parviz Kokhaei4, Mehdi Vazirian5 and Ali Akbar Shabani1,3*
... were analyzed using gas chromatography-mass spectrometry (GC-MS). Concentration-dependent scavenging of 2, 2-diphenyl-2-picryl hydrazil (DPPH) was seen across all three extract types (ethanol, water, and chloroform) with the IC50 values of 42.30 ± 2.35, 54.11 ± 3.62, and 85.71 ± 4.83 μg/mL, respectively which were higher compared to the positive control (ascorbic acid = 8.07 ± 0.30 μg/mL) and (butylated hydroxyanisole or BHA ...
Arslan Muhammad Ali Khan1, Rao Zahid Abbas1*, Zia ud Din Sindhu1, Muhammad Shahid Mahmood2
...ma ticks in bovines. Gas chromatography-flame ionization detection (GC-FID) was performed to identify the chemical components of A. subulatum essential oil. The acaricidal and repellent activities of the A. subulatum essential oil against Hyalomma spp. were observed via adult immersion test (AIT), the larval immersion test (LIT), egg hatchability test (EHT), and the tick repellency assay. GC-FID provided that the main compounds of A. subulatum essential oil we...
Khansa Jamil1, Muhammad Ramzan Khan1, Asad Jan2 and Ghulam Muhammad Ali1*
...ic extract by thin-layer chromatography and a total of 70 compounds were identified by GC-MS analysis. The phytochemical screening showed that the methanolic extract of the whole plant was rich in alkaloids and tannins. Zones of inhibition around the bacterial colonies show the antibacterial activity of the plant. Extract of the targeted plant was effective against all test organisms Escherichia coli, Bacillus, Staphylococcus, and Pseudomonas that revealed met...
Xiao-Wei Huang1,2, Mei-Li Liu1, Jin-Ji Wang1, Yue-Xin Liu3, Zhe Lin1, Chun-Shu Rong4* and Ji-Xiang Ren4*
...-high performance liquid chromatography (PD-HPLC). Wistar rats were randomly divided into 5 groups (n=10 per group): control, model, piracetam (PIR), VAPH (300 mg/kg VAP) and VAPL (200 mg/kg VAP) groups. After intragastric administration, the rats’ behavioral indexes were observed through the open field test (OFT), and the levels of superoxide dismutase (SOD), malondialdehyde (MDA), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) ...
Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3
...mobilized metal affinity chromatography by using His-Ni resins to get desired protein and characterized qualitatively by SDS-PAGE by using His antibodies and western blotting. Once purified the immunogenicity of rOmpH was evaluated in BALB/c mice. The expression of recombinant OmpH protein (rOmpH) resulted in its accumulation in cytosol. The purified rOmpH protein had a molecular weight of 36 kDa and concentration around 0.973 mg/mL. The BALB/c mice group inje...
Nauman Jamil Khan1, Rabia Tariq2, Hina Saleem2 and Muhammad Waheed Mushtaq2*
...absorption spectroscopy, chromatography methods, ion selective electrode method, laser-induced breakdown spectroscopy, complexometric titration method and thermometric endpoint titrimetry are developed to determine the quantity of sodium. Spectrophotometric methods are cheap, easy to handle and more precise to quantify sodium in food contents.
...
Rana Mahmood Ahmad*, Orooba Mohammed Maeed Ibrahim
...l smell and bud. The gas chromatography-mass spectrophotometer (GC-MS) method revealed the presence of sixteen distinct elements, making up 100% of all known compounds in their whole. The major ingredient, eugenol, made up 49.10% of the total compounds. Oil’s median lethal dose (LD50) was 2225 mg/kg, which suggests that clove oil has a relatively low level of toxicity. Eighteen adults male Wistar albino rats, six months, weighing 200–250g, were use...
N. A. Sherif, Lamiaa M. Omar, S. M. Shafei and Elham A. El-Ebiary.
...rified by using affinity chromatography. The obtained globulins were divided into 2 parts. The 1st one was conjugated with peroxidase and the 2nd part was conjugated with fluorescein isothiocyanate. Both conjugates gave a strong positive result when diluted 1:2500 and 1:50. respectively. The results indicated that they could be used successfully for detection of antigens and antibodies...
Nael Abutaha, Fahd A. AL-Mekhlafi*, Khalid Elfaki Ibrahim,
Mohammed S. Al-Khalifa and Mohamed A. Wadaan
...hemical analysis and gas chromatography-mass spectrometry (GC-MS). The cytotoxic effect was also evaluated using normal human umbilical endothelial cells (HUV-EC). The larvicidal assay was performed on the third instar stage as recommended by WHO. The phytochemical analysis showed the presence of alkaloids, sterols, and phenolic compounds. The GC-MS analysis revealed the presence of 5 compounds, of which α-Copaene constituted 61.95% of the extract. The h...
Olaolu Fadeyi1,2, Oluwatoyin Fabiyi3*, Tesleem Bello4 and Gabriel Olatunji5
...A. occidentale using gas chromatography spectrometry revealed the presence of several phyto constituents such as phenol, triterpenoids, flavonoids and other bio-active compounds. The array of fatty acid esters revealed in the GCMS result of the fractions especially fraction 52-71/Z2 is responsible for the exhibited toxicity to M. incognita with observed reduction in nematode population in roots and soil of the Okro plants. This resulted in increased fruit weig...
Qurrat ul Ain Shafique1, Sana Batool2, Hanfa Ashfaq2, Asima Tayyab2, Roquyya Gul3 and Mahjabeen Saleem1*
...mobilized metal affinity chromatography) was employed for the purification of IL-2. The refolding of the purified IL-2 was achieved by stepwise dialysis method using urea gradient (8M-0M). Human IL-2 was recovered from 1 litre culture to a purity level of ~95%. In vitro cytotoxic potential of IL-2 on HepG-2 and MCF-7 cell lines revealed that it possess sufficient cytotoxic potential and can inhibit the growth of these cell lines directly. Thus, refolded ...
Shah Zeb Khan1, Ali Hazrat1*, Farman Ali Khan2, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar3, Muhammad Ajmal Khan4 and Hayat Ullah1
