Ghulam Ali Bajwa and Muhammad Arshad Khan
...n of urea and diammonium phosphate (DAP). Urea at the rate of 608 kg/ha and DAP at the rate of 260 kg/ha was applied on six mulberry varieties. The results showed a deficiency in nitrogen, phosphorus, zinc and manganese in soil before the fertilizer application. Contrarily, high copper and potassium contents were found. After three weeks of fertilizer application, concentration of N, P, K and Fe in the soil almost doubled. Zinc and Cu contents decreased signif...
Mukhtarullah1, Jawad Ali2 and Mohammad Akmal1*
... (N 46%) and di-ammonium phosphate (N 18% and P 46%), each at sowing time during seedbed preparation and an extra bag of urea in February at stem elongation stage of the crop (each bag 50 kg ha-1). Rests of the cultural practices were kept common to all plots. Both sowing dates and varieties have resulted differences in plant height (cm), spikes m-2, spike weight (g), thousand grains weight (g) which affected the grain yield. Based on bio...
Sayeda Sarah and Muhammad Ibrar
...%, 50% and 100%) of rock phosphate (RP) fertilizer on spores density and percent root colonization of selected sunflower (Helianthus annuus L.) hybrids in P-deficient soil. It was observed that spore density and AMF root colonization was higher in the soil of control (RP0) plants, which decreases progressively with increasing fertility level. Less number of spores and percent root colonization was found at high RP level (RP100) in all hybrids. Higher P doses d...
Sanaullah Jalil*, Asim Hayat, Abid Majeed, Syed Haider Abbas, Muhammad Noman, Muhammad Imran Kasana and Muhammad Mazhar Hussain
...0 = control without rock phosphate & farmyard manure, T1= rock phosphate @150 kg P2O5 per hectare, T2 = FYM @5 tons per hectare, T3 = RP @100 kg P2O5 per hectare+ FYM @5 tons per hectare, E0 = without effective microbes and E1= EM-Biozote @ 50 l ha-1) on yield parametrs of wheat crop planted after the harvest of rice. All yield parameters significantly increased by the treatments consisting of RP (100 kg) with FYM (5 t h...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
...th normal saline (NS) or phosphate buffered saline (PBS), Protocol II (heating effect during incubation in tampon solution); smears were immersed in tampon either at room temperature or at 60oC, Protocol III (concentration of dye in staining solution); slides were stained with 1, 5, 200, 500, or 1000µg/mL solution. The slides were observed, intensity of staining and debris presence in samples. The debris presence was reduced (P<0.05) due to...
Muhammad Iqbal Anjum*, Shahbaz Javaid and Mukhtar Ahmad Nadeem
...ol with 0.83% di-calcium phosphate (DCP); R-II contained 50% less DCP (0.41%) than R-I and R-III contained R-II plus phytase Quantum® Blue @ 100 g/ton feed. Daily feed intake and weekly body weight gain per replicate were recorded for 35 days. On 35th day, blood and tibia bone samples from 5 birds per treatment were collected. Total weight gain and dry matter intake in chicks fed rations I, II and III were 1861, 1736 and 2023 gm/bird and 3242, 3...
Asma Akhtar, Abdul Rehman
...of incubation. Glucose 6 phosphate dehydrogenase (G6PDH) activity was increased to 279% (1f) and 150% (2b) when ompared to the respective control indicating the ability to tolerate tellurite tress. Differential pattern of bacterial proteins in tellurite stress was obtained by SDS-PAGE. Both bacterial strains can be utilized to convert toxic tellurite into its elemental form from the contaminated sites....
Nazakat Nawaz, Malik Shah Nawaz*, Nasir M. Cheema** and Mubashir A. Khan*
Corresponding author:nazakat_nawaz@yahoo.com
ZINC AND IRON APPLICATION TO OPTIMIZE SEED YIELD OF MUSTARD
...of Urea and triple super phosphate (TSP) with Zn and Fe. The increase in -1 Zn and Fe fertility from 0-1.5 to 5-1.5 kg ha increased yield of BARD-1. The maximum yield response was recorded when 5 kg ha-1 -1 Zn and 1.5 kg ha Fe were applied. Beyond this level, no further increase in yield was recorded in any mustard traits. A positive correlation was recorded between seed yield -1 -1 and 1000-seed weight with the application of 5 kg Zn ha and 1.5 kg Fe ha in co...
Muhammad Zahid Kiani*, Arshad Ali**, Tariq Sultan** and Muhammad Munir Ahmed***
CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA ISOLATED FROM ROOT SYSTEM OF SUNFLOWER (HELIANTHUS ANNUS L) GROWN UNDER SALT AFFECTED AREA OF PAKISTAN
...ubilization of inorganic phosphates, nitrogen fixation and siderophores and other organic acid production, whereas indirectly support plant growth by suppressing plant pathogens. The objective of this study was isolation and characterization of bacterial strains from rhizosphere, endosphere and rhizoplane of sunflower. Thirty six bacterial strains were selected out of 44 from plant root samples along with rhizospheric soil, collected from different salt affect...
Riaz Hussain*, Muhammad Riaz**, Mushtaq A. Saleem*** and Muhammad Ishaque Mastoi**
BIOCHEMICAL ABNORMALITIES INDUCED BY ABAMECTIN IN SIXTH INSTAR LARVAE OF THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM (HERBST)
...esistant (PAK) and organophosphate susceptible (FSS-II) strains of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) larvae in the laboratory. The objective was to examine changes in production or activities of carboxylesterase (CE), total esterases (TE), alpha-amylase, glucoamylase, alkaline phosphatase (AkP), acidic phosphatase (AcP), total protein, soluble protein and free amino acids (FAA). The sixth instar larvae of T. castaneum were released and e...
Riaz Hussain*, Muhammad Riaz** and Mushtaq A. Saleem***
TOXICITIES OF AZADIRACHTIN AND POLYCHLORINATED PETROLEUM HYDROCARBON AGAINST RESISTANT AND SUSCEPTIBLE STRAINS OF TRIBOLIUM CASTANEUM (COLEOPTERA: TENEBRIONIDAE) ADULTS
...ant (PAK) and organo- 50 phosphate-susceptible (FSS-II) strains of red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) adult beetles were determined through filter paper impregnated method against azadirachtin (Nimbokil 60 EC) and polychlorinated petroleum hydrocarbon (Tenekil 100 EC). The LC values of these insecticides were worked out as 12830 and 50 9331 ppm for azadirachtin and 5148 and 4047 ppm for Tenekil 100 EC against PAK and FSS...
Nadia Faqir1, Aish Muhammad2*, Ghulam Muhammad Ali2, Armghan Shehzad2, Hafeez Ur Rahman3, Farhatullah4 and Muhammad Zeeshan Hyder5
... k and geranyl geranyl biphosphate reductase) from chloroplast region. Analysis of variance has revealed significant variation among the studied date palm samples pertaining to the studied physical traits and chemical composition of fruit. Sequences of the maturase k gene has complete identity to the reference genome of ‘Khalas’ cultivar. While geranyl geranyl biphosphate reductase has one synonymous single nucle...
Farrukh Jamil*, Samra Raheel and Haroon Rasheed
...proteins and it may be a phosphate sensor.
...
Environmental Determinants of Zooplankton Community in the Damietta Estuary of the Nile River, Egypt
Wael S. El-Tohamy1,*, Russell R. Hopcroft2 and Nagwa E.M. Abdel Aziz3
...o salinity, temperature, phosphate concentration and phytoplankton biomass. The main spatial gradients along the estuary were associated with salinity. The high salinity zone in the estuary downstream was dominated by the calanoid paracalanidae and the harpacticoid Euterpina acutifrons. In the lower salinity transects, the tintinnid Favella serrata dominated the estuary midstream, together with several rotifer species in the estuary upstream. The...
Song Jiang1,2, Fa-lin Zhou1, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
...e (GSH) and adenosine triphosphate (ATP) concentration, as well as nitric oxide synthase (NOS) activity and nitric oxide (NO) concentration catalyzed by NOS. Based on biochemical analysis of the above, results showed that O2- production could be induced significantly after cold stress at 15°C and heat stress above 30°C. SOD activity showed a similar changing profile as the concentration of O2- after thermal s...
Asma Karim1, Wajid Ali1*, Noreen Ahmad1, Muhammad Irfan2, Hafiz Abdullah Shakir3
Histological and biochemical study of liver of silver carp (Hypophthalmichthys molitrix) after acute exposure to pyrethoride (deltamethrin)
...rganochlorine and organo-phosphate pesticides. Deltamethrin was selected to study the biochemical and histological
alterations in liver of silver carp. The LC50 1.6 μg/L was determined. Fish (n=6) were dividing into 4 groups A (control)
B, C and D (experimental). Experimental groups B, C and D were exposed to 25%, 50% and 75% of LC50
respectively for acute exposure. The blood and liver tissue sampling was done after 96hrs for histology ...
alterations in liver of silver carp. The LC50 1.6 μg/L was determined. Fish (n=6) were dividing into 4 groups A (control)
B, C and D (experimental). Experimental groups B, C and D were exposed to 25%, 50% and 75% of LC50
respectively for acute exposure. The blood and liver tissue sampling was done after 96hrs for histology ...
Jian Wang1,*, Zhi Liu2, Sheng Xu2, Mei-Fen Hu2, Xiao-Rui Liu1 and Wen-Jie Cai1
...ncoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference, the ratio of lgcDNA/lgGAPDH was regarded as the extreme level of CXCL8 mRNA. The serum level of CXCL8 and expression of CXCL8 mRNA in neutrophils of the chronic renal failure were (128.892±17.101) pg/mL and (1.332±0.138) lgcDNA/lgGAPDH. There was no significant difference compared with the normal controls (t=2.8468, P=0.062; t=1.953, P=...
Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2, Wardah Hassan3, Moazama Batool4, Shakeela Perveen5, Sadia Maalik4 and Sajida Mushtaq4
...e (endosulfan) and organophosphate (chlorpyrifos) investigated on the activity of antioxidant enzymes viz. superoxide dismutase, peroxidase, catalase and glutathione S-transferase in different organs (liver, gills, kidney, brain, heart and muscle) of fish, Labeo rohita. The LC50 of chlorpyrifos+endosulfan mixture was calculated as 1.95±0.02 μgL-1 for 96 h with the 95% confidence limits. The fish expose to the mixture (1:1...
Xiaopeng Tang1,2, Rejun Fang3,4,*, Guochang Pan1,2 and Kangning Xiong1,2,*
...l growth factor (EGF) on phosphate absorption across intestinal mucosa of hens using the Ussing chamber system. First, four Loman laying hens with similar weight (1.25±0.13 kg) were sacrificed to detect the lactate dehydrogenase (LDH) activity of isolated small intestine at time on 0 min, 30 min, 60 min, 90 min and 120 min, to provide a appropriate operation time for Ussing chamber test. Then, a total of 20 Loman laying hens with similar weight (1....
Matiullah Khan*, Shameemul Sibtain Shah, Atiqullah Khan, Muhammad Sarwar and Shoaib Ahmed
...icle size of Hazara rock phosphate (HRP) by manual preparation of Single Super Phosphate (SSP) through the procedure already established by the author and testing its efficacy in comparison to DAP on rainfed wheat. Almost 50 bags of SSP were prepared using formula “100 kg of indigenous HRP with total P2O5 content of X and mesh size 160 when treated with 60 liters (50% diluted) H2SO4 (volume/volume), followed by proper ...
Muhammad Izhar Shafi* and Muhammad Sharif
...ferent P sources as nitrophosphate (NP), single superphosphate (SSP), and di-ammonium phosphate (DAP) at a recommended rate of 90 kg P2O5 ha-1 were applied. Soils amended with different P fertilizers with and without HA showed mix trend in AB-DTPA extractable P at day zero of incubation and then a rapid mineralization and weekly turnover were increased up t...
Matiullah Khan1*, Motsim Billah2, Shoaib Ahmad1, Raza Ullah Khan1 and Muhammad Sarwar1
...ry litter (PL) with rock phosphate (RP) and inoculating with effective microorganisms (EM) on the wheat during 2010-11. Both experiments were conducted consecutively, in the same lay out of randomized complete block design with three repeats. Various doses of PEC (6, 4, 2 Mg ha-1) were compared with simple poultry-litter compost (PLC) as 8 Mg ha-1, single super phosphate (SSP) fertilizer as 100 kg P2O5 ha-1 and control. The ...
Imran1*, Amanullah1, Muhammad Arif1, Zahir Shah2 and Abdul Bari3
...with seed inoculation of phosphate solubilizing bacteria (PSB) (Pseudomonas) and phosphorus (P) on weeds frequency, and biomass at various growth stages of soybean and its yield contributing parameters. The consecutive field experiments for years 2016 and 2017 using randomized complete block design (RCBD) were conducted on soybean (cv. Malakand-96) crop. Experimental treatments included three organic sources, three phosphate...
Attique Ahmed1, Tariq Sultan1, Ghulam Qadir2, Obaid Afzal2*, Mukhtar Ahmed5, Shamim-Ul-Sibtain Shah3, Muhammad Asif4, Safdar Ali2 and Muhammad Zeeshan Mehmood2
...utrient uptake. PGPR and phosphate solubilizing bacteria (PSB) in combination with synthetic fertilizers can enhance crop productivity and soil fertility. Present experiment aimed to investigate the effect of PGPR and PSB inoculation with T1 Control (Recommended NP), T2 (PGPR-1 + PSB + ¾ N + ¾ P),T3 (PGPR-2 +PSB +¾ N + ¾ P), T4 (PGPR-3 + PSB + ¾ N + ¾ P), T5 (PGPR-4 + PSB + ¾ N + ¾ P), T6 (PGPR-5 + PSB + ...
Bin Huo1 and Xiaoyun Shen1,2,3*
...ion of disodium hydrogen phosphate in affected Tibetan gazelles successfully treated the illness. This study demonstrated that the ailment of the Tibetan gazelle was mainly caused by the phosphorus deficiency in forage.
... 
Nisar Mohammad٭, Mohammad Mansoor Khan٭, Noor Mohammad٭
EXTRACTION OF HIGH QUALITY TALC FROM TALC-CARBONATE ROCK OF MINGORA EMERALD MINES BY FLOTATION AND LEACHING
...frother, Sodium hexa metaphosphate as depressant for carbonates, equal mixture of oleic acid and kerosene oil as collector gave encouraging results. It was found that talc can be separated as float at pH 6 - 7±0.2 in rougher flotation and 10 - 11±0.2 in cleaning flotation, depressant dosage 0.10kg/ton, and collector dosage of 1.2 kg/ton. Talc concentrate at recovery of about 60 - 70% with a grade of 92.50% from an ore containing about 50 to 70% t...
Matiullah Khan1*, Humera Arif2, Muhammad Rasheed2, Motsim Billah3 and Shoaib Ahmad1
...on. The huge amount rock phosphate (RP) found in Hazara area of Pakistan can be utilized as P source by preparing Single Super Phosphate (SSP). The spatial variation in minerology of Hazara RP might have an impact on the availability of phosphorus from these rocks. The experiment was therefore planned to revisit the formulation of SSP for various Hazara locations’ rock, for obtaining maximum available P. Three main loc...
Mahak Fatima1, Memoona Syed1, Rabia Zeeshan2, Farah Rauf Shakoori3, Naveed Shahzad4, Moazzam Ali1 and Zeeshan Mutahir1*
...nucleotides to their monophosphate forms, thus preventing their incorporation into DNA and subsequently to oxidative damage. The present study explores the relationship between MTH1 gene silencing and its outcome on the growth of gemcitabine resistant breast cancer cells named MCF7-R. For this purpose, we transfected MCF7-R cells with MTH1 specific validated siRNA. Successful transfection of siRNA and consequent cytotoxicity in MCF7-R cells was c...
Sajid Amin Sarmad1, Muhammad Zeeshan Majeed1*, Muhammad Luqman1, Muhammad Asam Riaz1, Sohail Ahmed3 and Sylvain Nafiba Ouédraogo2
Development and Laboratory Evaluation of a Slow Release Formulation of Fipronil against Subterranean Termites (Odontotermes obesus Rambur)
...g organochlorines, organophosphates and pyrethroids. However, pesticidal control of termites is usually not persistent and long-lasting due to rapid decomposition and loss of insecticidal molecules active against target pests. This study was aimed to develop and evaluate a slow-release formulation (SRF) of a persistent insecticide (fipronil) against O. obesus. Technical grade insecticide fipronil was applied in the form of cellulose-made pellets made up of com...
Bilal Pervaiz Khan1, Neelam Ara1, Shujaat Ali1,2*, Kamran Khurshid Baigh1 and Abdul Wahab1
Effect of Seed Priming on Radish with Different Nutrients at Various Soaking Durations
... duration in Di-ammonium phosphate DAP solution. Incase DAP, showed excellent performance in germination, growth and yield. While KCl also gave better results in most of parameters....
Hafiz Hammad Ahmad1, Mukkram Ali Tahir1*, Noor-us-Sabah1, Ghulam Sarwar1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1 and Aneela Riaz4
Growth and Yield Response of Sunflower to Organic Amendments in Aridisol
...ve P source such as rock phosphate (RP) can offer a promising choice for sustainable P supply under P stress environment. A pot research was executed to explore the response of sunflower (Helianthus annuus L.) to sparingly soluble P sources grown under P deficient environment at research area, College of Agriculture, University of Sargodha during 2018. The experiment was laid out in a Completely Randomized Design (CRD) with eight treatments replicated thrice. ...
Hafiz Hammad1, Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Abid Niaz4 and Muhammad Arif4
...application of NK + Rock phosphate at 7 g/pot + Wheat straw at 28 g pot-1 (T6) in cultivar Hysun-33. While, the lowest value of P accumulation (89.13 mg P pot-1), P uptake efficiency (1.18) and PE ratio (605.0 g DM (g plant-1) was noted under NK + P0 (T1) treatment in Agwara-3. Among the tested sunflower cultivars, Hysun-33 showed superiority over Agwara-4.
...
Tasneem Shah*, Muhammad Tariq and Dost Muhammad
... urea for N, single superphosphate for P and potassium chloride for K) on soil respiration and N mineralization during lab incubation experiment under wheat-mungbean and wheat-maize cropping systems. The rate of CO2 evolution increased significantly (P<0.05) with increasing level of added biochar during each incubation period. At day 2, the rate of CO2 evolution increased from 142 µg CO2 g-1 soil d-1
Uwem E. George1, Oluwadamilola A. Arowolo2, Oluseyi A. Olayinka3, Ijeoma M. Ifeorah4, Temitope O.C. Faleye3,5, Bolaji Oluremi6, Arthur O. Oragwa7, Ewean C. Omoruyi8, Emmanuel E. Udoh9, Oluwadamilola G. Osasona1,10, Emmanuel Donbraye11, Olufunke P. Adeniji12, Olubusuyi M. Adewumi3,13* and Johnson A. Adeniji3,13,14
...led, then resuspended in phosphate-buffered saline. Subsequently, nucleic acid was extracted from the suspension and Illumina sequencing performed. ARGs in the raw reads were determined and assembled using the KmerResistance tool v2.2. From the 2,763,954 reads generated, 14 ARGs with statistically significant reads were identified. Precisely, 90.5% (12/14) of the ARGs detected target drugs that inhibit translation, of which 66.6% (8/12) were tetracycline resis...
Oluseyi A. Olayinka1, Temitope O.C. Faleye2, Oladipo O. Omotosho3, Oladapo A. Odukaye3, Bolaji Oluremi4, Ibipeju H. Ibitoye1, Oludayo O. Ope-Ewe1, Uwem E. George5, Oluwadamilola A. Arowolo6, Ijeoma M. Ifeorah7, Ewean C. Omoruyi8, Emmanuel Donbraye9, Olufunke P. Adeniji10, Olubusuyi M. Adewumi1,11* and Johnson A. Adeniji1,11,12
...ooled and resuspended in phosphate buffered saline. The suspension was then subjected to nucleic acid extraction, cDNA synthesis and Illumina sequencing. Antibiotic Resistance Genes (ARGs) in the raw reads were determined and assembled using the Kmer Resistance tool v2.2. From the 2,974,257 reads generated, 21 ARGs with statistically significant reads were identified. Almost all targeted broad-spectrum antibiotics with over 50% targeting Tetracyclines. Five (a...
Muhammad Adnan Islam1*, Zia-Ul-Haq2, Rana Shahzad Noor2, Matiullah Khan4, Muhammad Mohsin Ali1, Zulfiqar Ali3, Asif Ali Mirani3, Hafiz Sultan Mahmood1, Muzammil Husain1 and Badar Munir Khan Niazi1
...lizer such as Diammonium phosphate (DAP) conventionally applied to wheat by broadcast method before sowing crop. This is wasteful method of fertilizer application as only 15-20% of applied phosphate is utilized by the crop. To overcome the problems of phosphorus fixation, fertilizer band placement drill (FBPD) for wheat had been developed and its performance was evaluated at farmer’s fields. This drill places DAP ferti...
Mukkram Ali Tahir1, Noor-us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Abdul Moeez1, Muhammad Zeeshan Manzoor1 and Aneela Riaz3
... soil (4.93 dS/m). Si as phosphate industry waste was applied @ 0, 50 and 100 µg Si/g soil. Results showed that application of Si reduced the adverse impact of salinity and significantly enhanced the nutrients status of wheat plant. Under normal soil conditions, sodium (Na) concentration (5.63 mg/g), potassium (K) concentration (9.20 mg/g), Na/K ratio (1.60 mg/g), relative water contents (56.49%) and total chlorophyll contents (2.74 mg/g) were observed w...
Mukkram Ali Tahir1, Noor-Us-Sabah1*, Ghulam Sarwar1, Muhammad Aftab2, Abdul Moeez1, Humaira Ramzan1, Mudassar Hafeez1, Muhammad Zeeshan Manzoor1, Aneela Riaz3, Sher Muhammad4 and Muhammad Latif4
... soil (4.93 dS/m). Si as phosphate industry waste was applied @ 0, 50 and 100 µg Si/g soil. Results showed that application of Si reduced the adverse impact of salinity and significantly enhanced the growth of rice plant. Maximum height of rice plant (106 cm), fertile tillers number per spike (6), 1000-grain weight (21.76 g), shoot fresh biomass (25.62 g) and shoot dry matter (12.86 g) were found under saline environment when maximum concentration of Si ...
Muhammad Waheed* and Dost Muhammad
...sis either from RP (rock phosphate) or TSP (triple super phosphate fertilizer). The prepared composts were then applied to pots containing 6 kg of soil @ 160 mg P2O5 kg-1 and data were recorded on phosphorus fractionations at 1st, 5th and 10th week of germination and its correlation with wheat seedlings growth and leaf [P]. Results showed that the labile P fraction initially increased and then decreased and as compared to co...
Luis Daniel Jiménez Martínez1, Vicente Morales Garcia2, Carlos Alfonso Frias Quintana3, Alejandra del Carmen Castillo Collado1, Gloria Gertrudys Asencio Alcudia4, Carina Shianya Alvarez Villagomez4, Emyr Saul Peña Marín4,5, Bartolo Concha Frias1 and
Carlos Alfonso Alvarez-Gonzalez4
...i>) and glyceraldehyde 3-phosphate dehydrogenase (gapdh) in the different tissues of the intestine, muscle, gill, stomach, brain and liver in adult males of A. tropicus from reared in captivity through three BestKeeper, geNorm and NormFinder algorithms. Based on our results we can conclude that in the three BestKeeper, NormFinder and geNorm algorithms, the most stable genes are ef1, followed by 18s rrna and actb where the gen...
Warkoyo Warkoyo1*, Devi Dwi Siskawardani1, Anggit Ayu Pradana Siwi1, Moch. Wachid1, Ivar Zekker2 and Jumpen Onthong3
.../sub>) and Sodium Tripolyphosphate (STPP) — Na5P3O10 concentrations on the physical and mechanical edible film. Randomized complete block design (RCBD) factorial was applied with three replications. The 1st factor was glycerol concentration (17.5, 22.5 and 27.5) % v/w and the 2nd factor was STPP concentration (0.1, 0.2 and 0.3) % w/v. The results indicated a significant interaction between the additi...
Junaid Ahmad1*, Shazma Anwar1, Anwar Ali Shad2, Sher Shah Souri1, Bibi Amina3, Wajia Noor4, Abidullah1 and Muhammad Adil1
...lybdate and single super phosphate was used. The findings of trial indicated that molybdenum applied with 1.5 kg ha-1 to mungbean crop significantly improved pods plant-1 (29), seeds pod-1 (11), thousand seeds mass (38 g), biological and grain production (2960 and 777 kg ha-1) while highest nodules plant-1 (26), plant height (79 cm), protein (21.70 %), carbohydrates (60.53 %) and seed N content (3.76) was recorded with molybdenum applied at rate of 2.5 kg ha-1...
Syed Haseeb Ahmed Shah, Irfan Shahzad Sheikh, Abdul Samad, Muhammad Kamran Taj, Mohammad Masood Tariq*, Majed Rafeeq, Sumaira Fazal, Niamatullah Kakar, Sabeera Afzal and Asadullah
...tetracycline and Tylosin phosphate) were supplemented solely and in combinations with different concentration. Chicks were reared in floor pens bedded with saw dust litter, optimal husbandry conditions for broiler rearing were provided to the birds and vaccinations against Newcastle disease and Infectious bursal disease (IBD) were done at 9th, 19th and 11th, 23rd day, respectively. The results of current study revealed that there was non-significant difference...
Nafisa Shoaib* and Aisha Majid Ali
... concentrations of organophosphate pesticides (chlorpyrifos, malathion), synthetic pyrethroid pesticide (cypermethrin, lambda-cyhalothrin) and herbicide (buctril). Haemocytes of bivalve play an important role in immune defense and detoxification of contaminants. The bivalves were exposed to different concentrations of test pesticides in a static system. The MN frequencies of all the pesticide treated mussel groups increased significantly (p< 0.05) until the...
Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4
...easuring the calcium and phosphate ion concentrations by SEM/EDX analysis. While, the bioactivity of expanded and differentiated osteoblast cells derived from isolated rBM-MSCs by flowcytometric analysis was studied by histochemical staining with Alizarin Red and von Kossa to confirm the osteogenic differentiation process. Also, cell viability assay by MTT was used to measure the number of viable osteoblast cells cultured with scaffolds extracts. Also, the ant...
Ali Ghazi Atiyah1*, Nadia Hameed Rija Al-Falahi2 and Ghazi Atiya Zarraq3
...stify;">Beta calcium pyrophosphate (β-CPP) scaffold, a type of calcium phosphate-based biomaterials, can be used in orthopedic and dental surgery. This study focused on the synthesis of the β-CPP bone scaffold from waste biomaterials such as avian eggshell, which consider a natural source of calcium precursor. The β-CPP powder was prepared by a wet precipitation process using calcination temperature 1200 º...
Noor-us-Sabah1*, Mukkram Ali Tahir1, Ghulam Sarwar1, Muhammad Luqman2, Amir Aziz1, Muhammad Zeeshan Manzoor1 and Muhammad Aftab3
...rces of P, one is use of phosphate rock (PR). Pakistan is blessed with huge reserves of PR but poor solubility at higher pH makes it unsuitable for application as such. Thus, solubility of PR needs to be enhanced in a sustainable way. This review documented possible modes of PR dissolution for release of P. Possible means of PR dissolution and resultant P release include use of different organic amendments (e.g. farm yard manure, press mud, poultry dropping et...
Muhammad F Tajol Ariffin1, Chai M Hian1, Muhammad Z Sukiman1, Mohd F Ghazali1, Siti M Zainal Ariffin2*
...us aureus (S. aureus) or phosphate-buffered saline (control). The haptoglobin (Hp), serum amyloid A (SAA) and α1-acid glycoprotein (AGP) levels in serum and milk were measured at pre-and post-infection (6, 24, 48 and 72 h) using the ELISA method. The SCC was determined by the direct microscopic method. Results revealed a time-dependent change of Hp, SAA and AGP in the infected group compared to the control. The APP levels in the milk were significantly h...
Honnakerappa S. Ballari1 and Shashikant S. Udikeri2*
...hetic pyrethroids, organophosphates and carbamates was global issue in early nineties. Hence many new generation pesticides are in use widely. To know the status of different insecticides belonging pyrethroid, ogranophaopsahte and carbamate molecules monitoring was exercised using populations of H. armigera collected from different agroclimatic situations in Karnataka state, India. An abstinent to tremendous resistance was documented for synthetic pyrethroids,...
Roheela Yasmeen1* and Samia Chaudhry2
...rm water and chloroquine phosphate are some of the measures to mitigate the effect of cronavirus....
Weidong Huang1,2,3, Xinyue Liang1,2,3, Xiufeng Xie4, Xingmin Wang2, 3 and Xiaosheng Chen1,2,3*
...nuclear genes, carbamoyl phosphate synthetase (CAD) and histone subunit 3 (H3). Results indicated the average genetic distance was 0.005 among the different elytral forms of M. sexmaculatus based on combined dataset, which shows very close genetic relationships among them. Results also showed a high level of haplotype diversity (Hd = 0.902) and the low level of nucleotide diversity (Pi = 0.004). In addition, the number of haplotypes was 17 and the same elytral...
Serdar Bektas* and Ozer Ayik
...parameters and glucose-6-phosphate dehydrogenase activity, blood samples were taken 1,3,7,14 and 21 days after the infection. Total red blood cell values, were significantly decreased in 7 and 21th day, a significant decrease in hemoglobin concentration noted only in the 21th day, significantly lower values in hematocrit, were observed at 7, 14 and 21th days, total leucocyte and thrombocyte counts, found significantly higher only in the 3th day, comparing with...
Amjed Ali1, Muhammad Tayyab1*, Abu Saeed Hashmi2, Asif Nadeem3, Shumaila Hanif4, Sehrish Firyal1, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1
...7 °C in 50 mM sodium phosphate buffer pH 7. The presence of EDTA in the activity assay mixture reduced the β-lactamase activity to 91% while Zn2+, Co2+, Mn2+ enhanced the enzymatic activity to 144, 121 and 108% when used at a final concentration of 1 mM. The ionic and non-ionic detergents showed slight inhibitory impact on the recombinant β-lactamase activity. The enzyme exhibited the Km and Vmax values of 2.27 mM and 45.45µmol/min, respect...
Abdul Majid1*, Farah Naz1, Nasrullah Laghari1, Sanaullah Abbasi1, Sham Lal2 and Safdar Ujjan3
...was maintained in saline phosphate buffer (PBS) pH (6.4, 7.4 and 8.0), similar to that of water. Temperature based experiments depicted the resistance due to changes in the secondary structural conformation up to 50 °C, however such resistance was reversible after the cooling to 20 °C. In both solvents (PBS and water) lysozyme was predominantly folded to α-helical conformation. At 50 °C, helices were altered to turns. However, in presence of ...
Mohamed A. Hussein1, Bahgat A. Abd El-Rehman2, Kareem A. Eldin2, Mohamed Fouad Mansour1*
...noparticles and aluminum phosphate nanoparticles as adjuvants and evaluate their impact on biochemical, cellular and humoral immune response in sheep, The prepared vaccines were sterile, safe, emulsion is stable and validity within acceptable limit 0.02 EDF50/ml up till 15th month with AlP-NPsV and AlHV except Ch-NPsV that was valid up to 16th month. Lymphocyte count increased from the 1st week post-vaccination till the peak at 5th, 4th and 3th months in group...
Sara Mohamed Hemeda1, R.H. Sayed2, Hani Hassan1, Sheima A.E.2, Hassan Aboul-Ella3, R. Soliman3*
...skimmed milk and 5ppb in phosphate buffer saline. 75 cattle milk samples were examined by the developed kits and by the commercially available new existing and commercially available SNAP β-Lactam test kit. The results were compared, and the determined specificity, sensitivity, and accuracy of the locally prepared kits were 93.7%, 95.3%, and 94.6%, respectively.
Keywords | Polyclonal antibodies (PAbs) production, Nano-gold a...
Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6
...dministered with 2 mL of phosphate-buffered saline (PBS) via the intramuscular route. At week 2, both non-vaccinated and the vaccinated groups were challenged intranasally with 2 mL of bacterial cell suspension containing 105 colony-forming unit (CFU) of M. haemolytica serotype A2. Blood samples were collected on weekly basis. The results revealed that the plasma progesterone concentration increased significantly (p<0.05) whereas estrogen, follicle-stimulat...
Asma Fatima*, Ghulam Abbas and Shahnaz Rashid
..., transparency, ammonia, phosphates, nitrates, potassium, calcium, total alkalinity and total hardness were determined. Overall, 61 species of plankton have been recorded from the studied ponds and categorized into 19 groups; of which 25 phytoplankton species belonged to four major groups, whereas, 36 zooplankton species belonged to 15 groups. Among them, Bacillariophyta (69.815%) and copepods (78.927%) were “most abundant” as compared to other gro...
Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction
Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3
...n and were homogenate in phosphate buffer saline and centrifuged at 15000 rpm / 20 min that known as the crude antigen. It entered to affinity column chromatography [Cyanogen Bromide-Sepharose 4B (CNBr-Sepharose 4B)] (Sigma Chemical Co.), obtain the fraction. In brief, IgG of cows S. aureus positive sera were purified by precipitation with 50% ammonium sulphate followed by purification with Protein A Sepharose gel. The separated IgG was dialyzed for 3 days aga...
Irfan Shahzad Sheikh1, Syed Haseeb Shah1, Niamatullah Kakar2*, Mohammad Masood Tariq1, Muhammad Essa Kakar3 and Muhammad Zahid Mustafa1
...ydrate (OXY) and tylosin phosphate (TP) on different blood physiological and biochemical parameters in broiler chicken. AGPs supplement alone and in combinations were provided as pre-starter (days 1-12), starter (days 13 to 24) and finisher (day 25 to 42) feed. Standard broiler rearing temperature was provided in the shed. Results showed that the OXY and TP had significant (P<0.05) effect on physiology of blood parameters, mainly on hemoglobin (Hb) and eryt...
Sonia Sumreen1, Muhammad Sharif2, Tariq Sultan1 and Ijaz Ali1*
...P availability from rock phosphate (RP). To investigate the effect of RP augmented with two levels of HA on the productivity of wheat and P uptake, a field trial was conducted. Treatments used were Control (No RP, No HA), rock phosphate alone (RP), RP mixed with HA @ 5 Kg ha‒1 (RP+HA-5) and RP mixed with HA @ 10 Kg ha‒1 (RP+HA-10) and were replicated 3 times randomly in Complete Block Design. Addition of RP significantly...
Nina Chen1, Shuang Xu2, Juanjuan Lin1* and Moslem Lari Najafi3
...antibiotics, clindamycin phosphate, teicoplanin, trimethoprim/ sulfamethoxazole and micafungin sodium did not change significantly. The particle size of the mixture containing vancomycin hydrochloride, levofloxacin hydrate, metronidazole and fluconazole increased gradually. The particle size of fosfomycin sodium in the mixture containing gentamicin sulfate, abacain sulfate, minocycline hydrochloride, ciprofloxacin and/ or ciprofloxacin also changed significant...
Inam Ul Haq1*, Humara Umar1, Farah Umar2, Naeem Akhtar3 and Muhammad Jan4
...butyric acid (IBA), urea phosphate (UP) and paclobutrazol (PB). These chemicals were evaluated under seven treatments in controlled greenhouse environment and tested for different rooting parameters. The results obtained showed significant effect on rooting ability of the cuttings. The appealing results obtained at T6 (IBA+UP+PB) where maximum rooting 56.8% with average number of roots per cutting 4.4 and root length 4.3 cm was observed. This treatment also sh...
Muhammad Shahid Nadeem*, Jalaluddin Azam Khan and Firoz Anwar
... presence of pyridoxal 5 phosphate, alanine aminotransferase (ALT) is an enzyme which operates at the cross roads of amino acid and carbohydrate metabolism. The enzyme has been reported from a wide range of organisms including animals, plants, fungi and microbes. The enzyme has a clinical applications in the diagnosis of many diseases. In the present study we have produced a recombinant of ALT from Pyrococcus abyssi in BL21 (DE3) strain of E. coli. The recombi...
Kainat Bibi1*, Sajjad Khan1, Zulfiqar Ali Gurmani1, Fahad Karim Awan1 and Sajid Ali2
...zers urea and di-amonium phosphate fertilizers were applied at recommended doses. Data was recorded on various morphological, yield and quality parameters. The result revealed that organically sown wheat varieties took more days to anthesis and maturity while more tillers, biological yield, grains yield and leaf area were recorded in inorganic treatments. Quality of organic produced wheat was comparatively better than inorganic wheat as more 13.67 % more crude...
Rana Aamir Shehzad1, Ghulam Sarwar1*, Sabir Hussain Shah2, Mukkram Ali Tahir1, Noor-us-Sabah1, Sher Muhammad2, Muhammad Aftab3, Muhammad Zeeshan Manzoor1, Imran Shehzad1 and Usman Saleem4
... for P release from rock phosphate by wheat. The fourteen treatments with six replicates were arranged according to completely randomized design (CRD). Treatments of the study were T1 = Recommended N, K with no P, T2 = Recommended NPK from mineral fertilizer, T3 = T1 + all P from PROM (Phosphorus rich organic manure), T4 = T1 + all P from FYM , T5 = T1 + all P from compost, T6 = T1 + all P from press mud, T7 = T1 + half P from mineral fertilizer + half P from ...
Galuh Chandra Agustina1*, Erwin Syahfitri1, Viski Fitri Hendrawan1, Siska Aditya1,2
...>...
Xiaowei Huang1, Chao Ma1, He Lin1, Yuchen Wang1, Yan Xu1, Guangfu Lv2* and Zhe Lin1*
...and cyclic adenosine monophosphatec response element binding protein (CREB) in hippocampus were detected by western blotting. The results showed that, OR could significantly reduce the contents of CRH, ACTH and CORT in serum and improve the damage of hippocampal structure, and increase the expression levels of GR, MR, BDNF, TrkB and CREB in hippocampus. In conclusion, Oviductus ranae can reduce hypothalamic-pituitary-adrenal (HPA) axis hyperfunction induced by...
Mohammed Naji Odhah1,2, Faez Firdaus Abdullah Jesse1*, Bura Thlama Paul3,4, Bashiru Garba5, Zaid Mahmood1, Eric Lim Teik Chung6, Mohd Azmi Mohd Lila7
...lly with 2 ml of sterile phosphate-buffered saline (PBS-pH 7); Group B (Mycolic acid group) was inoculated intradermally with 2 ml of immunogenic Mycolic acid extract (1g /ml); while group C (Positive control group) was inoculated intradermally with 2 ml of 109 colony-forming unit of live C. pseudotuberculosis. All the animals were observed for 90 days post-inoculation. Blood samples were collected via the jugular vein from all the groups before the inoculatio...
Mehran Ali* and Inamullah
...sources {1. Single super phosphate (SSP), 2. Rock phosphate (RP), 3. Poultry manure (PM), 4. 50% SSP + 50% PM and 5. 50% RP + 50% PM} applied at the rate of 60 and 90 kg P ha-1. These treatments explored with and without incorporation of AM fungi. One control treatment was used for reference. The results exhibited that, AM fungi had non-significant effect on initial phenological stages of wheat like days to emergence and til...
Tehreem Iqbal1, Roheela Yasmeen1* and Faheem Hafeez2
...otal bilirubin, alkaline phosphate test, Lactate dehydrogenase, and alanine amino transferase) and kidney (Blood Urea Nitrogen, Creatinine, and Uric acid) biomarkers were assessed. The highest levels of ALT and ALP were linked to liver injury and a higher weight in the liver were observed in BPA treated rats as compared to the control group. Similarly, an increase in blood parameters were recorded in BPA treated rats followed by post -treated rats and pre- tre...
Ahmed Kadhim Munahi1* , Hameed A. Al-Timmemi2
...e treated with 0.2 mL of phosphate buffer saline. Chitosan nanoparticles were applied to the chitosan group with hemisection stie. From the first week to the completion of the experiment 16 weeks following surgery, the open field locomotor scale, which comprised gait, proprioceptive posture, and nociception pain, was utilized to assess motor and sensory gains. After 16 weeks of postoperative testing, the motor and sensory reflexes in the treated group were sub...
Seyed Ahmed Reza Hashemi1, Teymour Aminrad1, Asadullah Ali Muhammad2*, Mastooreh Doustdar3 and Parastoo Mohebi Derakhsh3
... tons. Contrast worth of phosphate showed substantial differences among diverse stations (P<0.05), while further parameters did not show substantial differences between stations (P>0.05). Multi-layer artificial neural network analysis indicated that two parameters, phosphate and nitrate, had the greatest impact on sea cucumber biomass. To implement a maximum sustainable yield method for this fishery in Iran....
Aatif Masood Ahmad Khan1, Masood Rabbani1*, Arfan Ahmad1, Muhammad Wasim2 and Sohail Raza1
...ide adenine dinucleotide phosphate (NAD) for their growth. The isolates were typical for Av. paragallinarum as they were not able to ferment neither galactose nor trehalose. Performing PCR of direct swab samples provided higher detection level as compared to the PCR from isolated colonies following conventional diagnostic procedure. In the current study, page serotyping demonstrated that all isolated Av. paragallinarum belonged to page serovars B. Among the me...
