Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
... the development of SCNT embryos in the BMI....
Madeeha Arshad1, Naveed Ahmad2,*, Muhammad Khalid3, Asmatullah1, Mohammad Tahir2, Khadija Naveed1 and Asia Iqbal4
...atogenic effects in mice embryos.
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
..., heat shock to immature embryos prior to infection, resting stage and Agrobacterium strains that deliver T-DNA into plant cell from which whole plant can be regenerated. The results indicated that optimal embryo physiological age is 15 and 18 days after pollination and transient transformation efficiency decreased at 22 days after pollination. Enhanced transient beta glucuronidase (GUS) expression with average frequency of 40.56% was noticed with three days o...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
... of raising the immature embryos as a starting material for genetic transformation. Unlike immature embryos, the development of coleoptilar node is seasonal free and green house independent which would accelerate the work relevant to genetic transformation of maize. MS medium fortified with 3 mgL-1 BAP and 5 mgL-1 picloram produced maize seedlings with expanded coleoptilar nodes. Longitudinal cut sections of expanded coleopt...
Karim El-Sabrout* and Mohamed H. Khalil
...ity (period length, dead embryos and percentage) and hatch chick performance (weight, vitality). The experiment was carried out in two groups with total number of 1800 eggs (900 eggs for each group). Eggs were incubated 12 h of light (natural) then 12 h of complete darkness (G1); and 12 h of light (natural) then 12 h of LED lighting (G2). From the obtained results, there were no significant effects of complement LED lighting” after effect on hatchability...
Justyna Batkowska1,*, Lukasz Wlazlo2, Kamil Drabik1, Bozena Nowakowicz-Debek2, Karrar I.A. Al-Shammari3 and Magdalena Gryzinska1
...e infertile eggs or dead embryos, as well as samples of eggs to microbial analysis were. After 17.5 days of incubation its results were evaluated. Chicks were reared for 14 days and their livability and body weight gains were registered. The effectiveness of grapefruit juice as a disinfectant for hatching eggs was noticed in terms of reducing the development of microflora on the eggshell. Its use did not directly affect the hatching results and chick quality, ...
Shagufta Andleeb*1, Shabana Shaukat1, Chaman Ara2
... groups. Chicks and dead embryos were studied for morphological and morphometric analysis. Liver tissues were dissected out from each group for histological analysis. Cadmium chloride induced significant developmental abnormalities such as reduced body weight and crown rump length, exencephaly, ablepharia, crossed beak, gastroschisis, crooked toes, non-alcoholic fatty liver and pyknosis. Fresh garlic juice has successfully attenuated these toxicities and incre...
Shagufta Andleeb*1, Fariha Zahid1, Noor-ul-Ain1, Yousra Nisar1, Chaman Ara2
...p> day while recovery of embryos was done on 7th day of incubation. Disrupted embryos with microcephly, hydrocephaly, short neck, micromelia, amelia, micrognathia, agnathia, cataract, ectopiacordis, omphalocoel, axis distortion, anencephaly, anophthalmia, microphthalmia, phocomelia with reduced body weight and crown- rump length in all dose groups were obtained. Adverse histological changes appeared in the form of...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...icantly increased in the embryos derived from oocytes matured in 20% and 40% SFF supplemented media. HSPB1 expression was significantly increased in group matured in 20% SFF. The relative expression of IL-6, LAMA1 and FGF4 genes did not show any difference between groups. In conclusion, IVM medium supplemented with 10% SFF showed the best rate of blastocyst development.
...
Xu Lijie, Obaid Ullah, Liu Haixing, Ihsan Ali, Zhongshu Li* and Nan-Zhu Fang*
...ammalian preimplantation embryos. Peroxisome proliferators-activated receptor gamma (PPARγ) functions in the nuclear regulatory factor 2 (Nrf2) antioxidant pathways by binding to the promoters of antioxidant genes and regulates the expression of genes that associate with NADPH oxidase. To understand the role of PPARγ in the development of early embryos and define the mechanism responsible for the arrest in develo...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*
...tal efficiency of cloned embryos from somatic cells remains low, and many factors contribute to overall development of the embryo, including gene expression by the donor cells. The human hematopoietic stem/progenitor cell 117 (HSPC117) protein has been identified as being involved in placental formation, and can be modified by epigenetics. However, whether HSPC117 affects development of cloned embryos mRNA expr...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
... porcine parthenogenetic embryos experienced different types of microopetation using Raman spectroscopy. The results showed that Group II with the puncture of egg zona and plasma membrane had the highest cleavage rate and blastocyst rate of porcine parthenogenetic embryos, which was significantly higher than Group I with intact oocytes (P<0.05) and Group III with the puncture of zona and plasma membrane and aspiration egg...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
...ent of Nili Ravi buffalo embryos. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium al...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
... and maintaince of early embryos in buffalo.
...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...ytes and resultant early embryos. Germinal vesicle (GV) oocytes were first cultured to monitor the progression of germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) during in vitro maturation (IVM), then the harvested metaphase II (MII) oocytes were parthenogenetically activated to evaluate pronuclear (PN) formation of parthenogenetic embryo and embryo development. The cytoplasmic maturation was examined by measuring the intracellular reac...
MA Liman1, QI Yongxiao1, Wang Wenji2* and Zhong Qianyi3*
... G-Rh2-treated wild-type embryos were performed to evaluate the toxicity of the compound. Tg(fli1-EGFP) transgenic zebrafish with fluorescent blood vessels were studied to evaluate vascular development defects. G-Rh2 treatment up to 84.85 μM did not induce morphological defects in zebrafish. We observed suppression effect of angiogenesis by G-Rh2 in inhibiting intersegmental vessel formation from 30 to 84.85 μM in a dose-dependent manner. M...
Zulekha Zameer, Samreen Mohsin, Ammarah Hasnain, Asma Maqbool* and Kauser Abdulla Malik
Influence of Explant Sources on in vitro Callogenesis and Regeneration in Maize (Zea mays L.)
... especially the immature embryos. However, the manipulation of immature embryos as explants is hampered due to its unavailability throughout the year and low regeneration response. The present study is aimed to investigate the effect of various explants sources for callogenesis and regeneration in maize (Pioner 3025). The main explants under consideration were: three parts of germinated seedlings, mature embryo and split see...
Fuhua Zhang, Yishuang Yu and Shibao Wu*
...ultrasonography revealed embryos in pangolins MJ-X2 and MJ-X3, but not in MJ-X4. Pangolins MJ-X2 and MJ-X3 gave birth to cubs 102 and 123 days, respectively, after B-ultrasound detection; pangolin MJ-X4 did not give birth to a cub. This study showed that B-ultrasound can accurately diagnose pregnancy in Sunda pangolins and detect the developmental status of their embryos and fetuses. As a method of pregnancy diagnosis, B-ult...
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...pment potential of early embryos by promoting the cytoplasmic maturation.
... 
Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3*
...ality of parthenogenetic embryos as a source of pESC primary colonies. Fertilized embryos as positive control group. Diploid parthenogenetic embryos were divided into three treatment groups: CHIR99021 0 mmol/l, CHIR99021 0.003 mmol/l, and CHIR99021 0.01 mmol/l. The embryonic stem cell (ESC) primary colonies were cultured from blastocyst stage embryos. Th...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3*, Abd El-Nasser Ahmed Mohammed3
...tential of the resulting embryos in vivo and in vitro. Because of the importance of cumulus cells on follicular development, this review was made to collect and consolidate the current knowledge of cumulus cells effects on maturation of intact and reconstructed oocytes and further developmental competence of embryos in vivo and in vitro.
Keywords | Follicle, Cumulus cells, Follicular fluid, Cytoplast, Nucleus, Nucleo...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
...ggs (SPF-ECEs). The dead embryos showed hemorrhages, congestion and dwarfism. The collected allantoic fluids were examined later by haemagglutination (HA) and haemagglutination inhibition (HAI) test. The allantoic fluids of 5 pooled samples expressed positive using specific Newcastle disease virus (NDV) antiserum. The NDV field isolates were confirmed by reverse transcription polymerase chain reaction (RT-PCR) and partial sequencing targeting F gene of amplico...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Al-Hassan Mohammed4*
...lopment of the resulting embryos. Because of the significance of oocyte maturation stage on further embryonic development, this review aims to discuss the knowledge of oocyte maturation and its effect on further development of embryos in vivo and in vitro.
Keywords | Oocytes, Cumulus cells, Cytoplast, Nucleus, Embryos
...
Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*
...f 14-day-old Peking duck embryos using the fibronectin adhesion method. The morphology of CPSCs was observed under an inverted microscope. Based on the growth curve test, we found that the growth mode of the cells was “S” type growth. The clonogenic assay was performed to determine the clonogenic efficiency CPSCs. The expression of cell surface markers was detected by immunofluorescence, flow cytometry, and RT-PCR, and it was found that the CPSCs p...
Ghulam Jelani1, Qudratullah Kalwar1*, Asmatullah Kaka2, Abdullah Channo2, Ayyaz Ahmed1, Majid Hussain Soomro1, Hidayatullah Soomro1, Deepesh Kumar Bhuptani1 and Yar Muhammad Jalbani1
...ul transfer of mammalian embryos in 1890, it was approximately 60 years before significant progress became quite noticeable in the basic technology of embryo transfer in cattle. In embryo transfer process, embryo is collected from superior quality donor cattle and transferred to other recipient female cattle for complete development unless the gestation accomplishes. This technology involves the selection of donor and recipient animals, management for better b...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
... transfer, germ cells or embryos’ micromanipulation, sperm sexing, cryopreservation of ovarian tissues and germ cells and embryos, and genome resource banking for mammalian species. Such biotechnologies allow more offspring to be obtained from selected parents to increase milk and meat productivity, reduce the interval between generations, therapy of diseases and conservation of endangered mammalian species. Practicall...
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
Najamuddin Solangi1*, Mushtaque Ahmed Jatoi1, Adel Ahmed Abul-Soad2, Abdul Aziz Mirani1, Muhammad Aslam Solangi3 and Ghulam Sarwar Markhand1
...ficantly highest somatic embryos in cvs. Begum Jungi (83.3%) and Ajwa (82.6%). Somatic embryos induced in calli after nine months of initial culture were categorized into repeated and non-repeated. Medium comprising of NAA 0.1 mg L-1, Kin 1.0 mg L-1 revealed with significantly highest germination of somatic embryos in cvs. Begum Jungi (55.6%) and Ajwa (52.3%). Findings obtained will suppor...
M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1
...TUNEL staining in bovine embryos produced in vitro from oocytes matured in stressful conditions of elevated concentrations of non-esterified fatty acids (NEFAs). The maturation conditions in the current study were resemble the situation in the oocyte microenvironment of metabolically stressed lactating dairy cows during the post-partum period where the negative energy balance occurred. Oocytes were in vitro matured under elevated levels of NEFAs for 24 h in se...
Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*
...scriptome between bovine embryos of different genders. In this study, we performed transcriptome analysis of cattle embryos based on a single Cell RNA-Seq. Bovine sex-controlled semen for artificial insemination were used to obtain different stage embryos: bovine 8 cell XX embryo, 8 cell XY embryo, 16 cell XX embryo, 16 cell XY embryo, morula XX embryo, morula XY embryo, blastocyst XX embr...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
...here were 228 developing embryos (41%) after 48-hour of culture with 2-, 4-, 8-, and 16-cell division. EGF concentrations in the medium of 2-cell to 16-cell ranged from 0.8539 ng/mL-0.2838 ng/mL. The concentration of FGF2 in follicular fluid is 0.629 ng/mL, while the concentration of FGF2 in maturation media, culture media 2 cells, 4 cells, 8 cells and 16 cells respectively, namely 0.032 ng/mL, 0.015 ng/mL, 0.011 ng/mL, 0.009 ng/mL and 0.008 ng/mL. In conclusi...
Erni Damayanti1, Herry Sonjaya2*, Sudirman Baco2 and Hasbi Hasbi2
...de (H2O2) in Bali cattle embryos produced in vitro. 542 oocytes were matured in TCM 199 medium, were fertilized and were cultured using CR1aa medium for 48 h. After 48 h of culture, the embryo developed into 2, 4, 8 and 16 cells. Furthermore, it was grouped based on the stage of development, each of which was re-cultured for 48 h. After that, its development was re-evaluated and H2O2 measurements were taken. The 2-cell embryo was not able to reach the morula, ...
Rudhy Gustiano1*, Iskandariah Iskandariah2, M.H. Fariduddin Ath-Thar1, Gleni H. Huwoyon4 and Deni Radona3
...ance of fertilized eggs, embryos, and larvae showed no differences between observed populations. The survival rate of larvae for East Java, West Kalimantan and Lampung were 92%, 86% and 82%, respectively....
Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.
...ities up to 100% for the embryos 24-48hrs post inoculation and gave very strong RBCs agglutination. The haemagglutination positive samples were identified and subtyped antigenically by serological tests using Haemagglutination inhibition test (HI) using standard Al antisera and genetically by RT-PCR using specific primer. All the isolates were confirmed to be H5N1 and grouped according to the year of isolation as 2006, 2007, 2008, 2009 and 2010 group isolates....
Madboulyl , H. M.; Tamami , S.M.; Abd Elmoneml , A.S.; Husseinl , A.S. and Arafa2, A.M.
... SPF-ECE of 5-7 days old embryos via yolk sac route. Characterization of isolated virus was done by using RT-PCR where the 5 isolates were positive for APV and produced a specific 315 bp products. Serological survey for APV antibodies in serum and egg yolk samples collected from non-vaccinated flocks (608 serum samples were obtained from broiler chickens from El-Fayoum, Beni suef and Giza, 40 turkey poults serum samples from Al Behira and El Monofia, 278 chick...
Abou zead, A.A., Ali, A.A.H.*and Abd El Hkeem M.M.
...on on the CAM of chicken embryos revealed the isolation of 3 IBD viruses from diseased quails. Isolated IBDV viruses '.vere examined by electron microscopy. The disease was reproduced in quails to record the course, the clinical findings, post mortem and histopathological changes. Also seroscreening of serum samples by AGPT were done. The present findings focus the attention to the importance of the quails from the epidemiological point of view as potential so...
S. A. Sidaros*, S. A. EL-Kewey*, Eman A. H. khattab**, M. M. ELsharkawy*
...ed coats, cotyledons and embryos) of immature seeds obtained from infected faba been and cowpea plants. Both of the viruses were not detected in seed coat of ripened seeds and in roots of infected plants. BBSV was detected in stained seeds more than un-stained seeds obtained from infected faba bean plants. ...
