Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
...the expression of the proapoptotic protein (Bax) and the expression of macrosialin cluster of differentiation (CD68). APAP elevated of most of the previously measured parameters and decreased GSH, SOD, and total protein levels. Liver sections of H&E demonstrated liver injury characterized by centrilobular hepatocellular necrosis, CD68, and Bax expressions were also increased. Treatment with all the aforementioned antioxidants downregulated most of the elev...
Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*
...al changes and increased apoptotic cell death in splenic tissue of infected mice. Moreover, infection with live P. chabaudi was accompanied with an increase in NO, H2O2, and MDA levels in splenic tissue of lupus mice in comparison to either control or gamma irradiated P. chabaudi- infected group. Our data reveal that infection of lupus mice with live P. chabaudi has several effects on the splenic tissue on the histological and biochemical levels. Parasite irra...
Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3
...atalyze metabolism of proapoptotic ceramide to anti-apoptotic sphingosine and other fatty acids. In this study we have evaluated the pro-apoptotic effect of ceranib-2, a human ceramidase inhibitor and of a short-chain C2 ceramide on human lung adenocarcinoma cells, A549 by determining cytotoxicity of these agents by trypan blue assay, apoptosis inducing ability by annexin V and JC-1 staini...
Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
... licofelone caused early apoptotic cell rates of 4, 15 and 24 % for 24 h and 6, 13 and 24 % for 48 h, respectively. In addition, 150, 200 and 250 µM of 5-Fluorouracil resulted early apoptotic cell values of 13, 12, and 11 % for 24 h and 18, 22 and 16 % for 48 h. This study revealed that licofelone possesses dose and time dependent anti-proliferative and apoptotic properties on hepato...
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
...ebbing and generation of apoptotic bodies by cell fragmentation were evidence of apoptosis. In pre and postovulatory follicles, karyorhexis and the budding phenomena were clearly detected. Karyorhexis and budding phenomena were also the sing of apoptosis. Atresia was also clearly detected in pre and postovulatory follicles. The detection of karyorhexis, characteristic of apoptotic cells may provide a better understanding in ...
A.M. Abdul Azeem, A.N. El Shahat* and Ashraf M. Mounir
...meliorate the damage and apoptotic effects occurred by exposure to toxic dose of lead.
...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
...NCaP cells for 24 h, the apoptotic values (0.60% for the control group) were found 2%, 4%, and 17%, whereas the values for 48 h were 10%, 10% and 64% (0.48% for the control group), respectively. TA induced apoptosis of LNCaP cells depending on the dose and duration of exposure. According to PCR results, caspase-9 activity increased incrementally in groups given 50 μM of TA. The increase in the apoptosis of LNCaP cells may be due to the rising amount of TA c...
Zijuan Li1,2, Rong Ma2, Muhammad Khan3*, Chenchen Liu2, Xiaolin Cui2 and Yongming Li1,2*
...l significantly enhanced apoptotic efficacy of CDDP in U2OS cells. On the basis of our findings, piceatannol is a promising anticancer agent which could be developed into lead for osteosarcoma treatment as a single agent or in combination with CDDP.
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Feng-Mei Yang1, Rui Li2, Xiu-Xue Hu3, Yong Liang4, Bo Gao3* and Wei Chen3*
... increasing of CPIRs and apoptotic rates; The relative expression levels of β-catenin and Senescence marker protein-30 (SMP30) gene in UVB treated group were significantly lower than that of the control group; Gene expression levels also varied with different UVB doses and durations, indicating statistically significant dose- and time-dependent effects of UVB. UVB treatments inhibit cell growth and promote apoptosis in a dose-dependent and time-dependent ...
Canan Vejselova Sezer*
...t was shown clearly that apoptotic cell death hallmarks in silymarin and silymarin-loaded solid lipid nanoparticles treated cells, being more prevalent for the latter. These findings suggested improved bioavailability of silymarin in nanoparticle formulation, consequently silymarin-loaded solid lipid nanoparticles could be considered as a useful drug delivery system for silymarin that has poor water-solubility and an excellent candidate for anticancer drug dev...
Kanwal Haneef1,2, Rakhshinda Habib1,3, Nadia Naeem1,3 and Asmat Salim1,*
...ion, proliferation, anti-apoptotic and proangiogenic properties. MSCs have the ability to fuse with somatic cells in vivo and depending upon the microenvironment, the fusion products may perform tissue-specific function. Stem cell factor (SCF) is one of the important cytokine for cardiomyocyte survival following cardiac injury. The main objective of the present study is to determine the fusion potential of SCF transfected MSCs and cardiomyocytes (CMs). ...
Junli Liu1, Tongfang Yang2 and Lisheng Wu3*
... P21, Caspase-3 protein, apoptotic rate, and miR-340 expression in U2OS cells, and obviously decreased the cell survival rate, the number of colony formation, and the expression of LINC00857 (P<0.05). All were dose-dependent. LINC00857 targets and regulates miR-340. Overexpression of LINC00857 or inhibition of miR-340 evidently reduced the expression levels of P21, Caspase-3 and apoptosis in U2OS cells after homoharringtonine treatment, and remarkabl...
Ji Xu, Zhonghua Liu, Zhenhai Cui and Wenhai Zhao*
...y was used to detect the apoptotic rate. The expressions of Bax, cleaved-caspase3 and Bcl-2 were detected by Western blot. qRT-PCR was used to detect the effect of GSTT on miR-99a expression level. The above methods were used to detect the effects of miR-99a overexpression on IL-1β-induced chondrocyte inflammatory factors and apoptosis rate. Anti-miR-NC and anti-miR-99a were transfected into chondrocytes, respectively, and treated with a culture solution ...
Tao Ren1, Guiqiu Cao2, Xiao Han3, Feng Tan1, Qiaoli Chen4, Shicheng Yang5 and Haiyan Zhang6*
Chinar Mustafa Mohammed1*, Thamer M. Bashir1, Omar A.M. Al-Habib2, Farhad Ramadhan1, Mohammed Bassam1
...ium homeostasis, and antiapoptotic effects. There is mounting evidence supporting the utility of taurine as a pharmacological agent against heart disease, including chronic heart failure. This study investigated the potential protective effects of taurine, on behavior and cardiovascular function in the male rat following arrhythmia induced by the social stressor. Here, we studied levels of heart electrical activity following experimental conditions: Cage contr...
Asmaa Khamis1, Osama Abdalla1, Mohamed Hashem2, Noha Abdelnaeim1*
...yde (MDA), antioxidants, apoptotic markers, cytokines, and histopathological changes. Our results revealed a significantly increased MDA, caspase-3, p53, tumor necrosis factor-α, interleukin (IL)-1β, IL-18 and, IL-10, and a significant decline in glutathione and superoxide dismutase in the CCl4 group. However, intoxicated groups treated with PN and PM showed marked improvement in the measured parameters. Therefore, PN and PM have antioxidant and ant...
Guang Yin, Wencheng Kong, Yuqiang Shan, Jian Zhang, Rongchao Ying and Sixin Zheng*
...ntiproliferative and pro-apoptotic effect of cisplatin on Hep-3B cells. Double luciferase reporter gene experiments confirmed that TINCR can target miR-646. We concluded Down-regulating the expression of TINCR can inhibit the proliferation, invasion and migration of hepatocellular carcinoma Hep-3B cells and enhance its sensitivity to chemotherapy with cisplatin, and the mechanism may be related to the targeted regulation of miR-646 expression....
Hairul Islam M. Ibrahim1,2, Abdalla A. Sayed1,3, Abdel Naser A. Ahmed4,5 and Emad A. Ahmed*1,6
...ted the antioxidants and apoptotic markers including Caspase-3, TGF-β, SIRT-1, CCL-2 and MBP. Moreover, PCB modulated the levels of inflammatory mediators and TGF-β in splenic cells. The production of myelin basic protein (MBP) increased in PCB treated EAE mice. Taken together, the current finding revealed that, PCB suppressed EAE pathological checkpoints for demyelination and apoptosis in CNS and act as a therapeutic role for treatment of MS in upco...
Eman A. Al-Shahari1,2, Eman R. ElBealy3, Abdelhalim A. Alkhazendar4 and Abeer A. Alm-Eldeen4*
...se the expression of pro-apoptotic proteins p53 and bax and increase the antioxidant defense of hepatocytes in aged male rats....
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
...n factor (ELL2), and pro-apoptotic Bcl-2 homology 3-only protein (BIM) in MCF-7 breast cancer and normal human embryonic kidney HEK-293 cell lines were studied in response to treatment with different concentrations of UNC0642. Inhibition of G9a expression was observed in a dose-dependent manner in both cell lines with increasing UNC0642 concentrations. A dose-dependent downregulation of gene expression of proliferation marker genes (FGF-1, CCND1, and ENO2) was...
Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1*
...he expression profile of apoptotic genes (TNFα- BAX - CASP3 - FASLG - AGTR2 - and NOS2) mRNAs during early, mid, and late stages of CL and (2) clarify the accompanied changes in progesterone (P4), nitric oxide (NO) concentrations and histological evaluation through different stages of CL in buffaloes. Forty five paired samples of ovarian tissue from buffaloes were obtained from slaughterhouse. Ovaries were collected and CLs were classified morphologicall...
Magda A. Eldomiaty1,2, Manal E. Elsawaf2, Soad S. Ali3, Heba El-Sayed Mostafa4,5*
...se in the number of both apoptotic and proliferative cells was seen with significantly higher mean number of the proliferative cells. By EM, the nuclei were shrunken, secretory vesicles were depleted, and the smooth endoplasmic reticulum showed massive dilatation. These findings were partially restored in rats of group IV. This study concluded that voluntary exercise enhanced recovery from the injurious effect of depression on adrenal cortical cells that parti...
Lihang Wang, Qiling Chen, Tingsheng Lu, Shudan Yao, Xingwei Pu and Chunshan Luo*
...could effectively reduce apoptotic cells, dramatically minify TNF-α, IL-6, IL-8, MDA and Bax contents (P < 0.01) and elevate GSH-Px and Bcl-2 level (P < 0.01). Additionally, the P38AMPK/Smad2 pathway proteins (phosphorylated p -P38MAPK and Samd2) were notably higher in the model versus the control and the sham, while MH and MH+MPS could patently down-regulate p-P38MAPK and Samd2 protein levels. To conclude, MH+MPS is available to refrain the activa...
Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1
...umor growth factors, and apoptotic TUNEL assay, were evaluated after three weeks in addition to Immunohistochemical analysis was done on the proliferating cell nuclear antigen (PCNA), hypoxia-inducible factor 1-alpha (HIF-1), PPAR, and cyclin D1. Results: Mice treated with Cis+Pio HD showed higher levels of SOD, GSH, p-ERK1/2 and p38-MAPK, as well as lower levels of MDA and tumor growth factor beside the higher apoptotic act...
Lei Jiang, Yi Huang, Runfeng Yang, Xiaohui Jiang* and Hao Yan
...etect the expressions of apoptotic proteins Bax, Bcl-2, cleaved caspase and PAX2 in SKOV3/DDP cells, and the targeted relationship between circ_0009910, miR-455-5p and PAX2 was verified by double luciferase. Our results showed that circ_0009910 and PAX2 genes were significantly up-regulated in SKOV3/DDP cells, and miR-455-5p was significantly down-regulated in SKOV3/DDP cells. circ_0009910 could enhance cisplatin sensitivity of SKOV3/DDP cells. circ_0009910 ta...
Dalia M. Amin1*, Noha Mohammed Halloull1, Walaa E. Omar2, Basma A. Ibrahim2, Nahla M. Ibrahim1
...ic, antioxidant, and antiapoptotic pathway capabilities.
...
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M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1
...research aimed to assess apoptotic cell ratio (ACR) via TUNEL staining in bovine embryos produced in vitro from oocytes matured in stressful conditions of elevated concentrations of non-esterified fatty acids (NEFAs). The maturation conditions in the current study were resemble the situation in the oocyte microenvironment of metabolically stressed lactating dairy cows during the post-partum period where the negative energy balance occurred. Oocytes were in vit...
Yakun Ge
...expression levels of pro-apoptotic proteins Bax, caspase-3 and caspase-9, inhibit the expression level of anti-apoptotic protein Bcl-2 and the epithelial-mesenchymal transition of cancer stem cells, reduce the proliferation ability of cancer stem cells, and increase the apoptosis of cancer stem cells....
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
...alpha; (TNF-α) and apoptotic marker Caspase-3 compared to the control group. Rats received BPA along with SeNPs-CP showed significantly less severe damage and remarkable improvement in all the measured parameters when compared to BPA-rats. In this study it was concluded that the synthesis of selenium nanoparticles using a natural source such as grapefruit was effective in combining the antioxidant and anti-inflammatory properties of both the flavonoids p...
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
...alpha; (TNF-α) and apoptotic marker Caspase-3 compared to the control group. Rats received BPA along with SeNPs-CP showed significantly less severe damage and remarkable improvement in all the measured parameters when compared to BPA-rats. In this study it was concluded that the synthesis of selenium nanoparticles using a natural source such as grapefruit was effective in combining the antioxidant and anti-inflammatory properties of both the flavonoids p...
Salma Jameel Askar
...liferation, enlarged and apoptotic hepatocytes. Liver sections of G3 showed less side effect than Meloxicam-treated group. From this study concluded supplementation with beetroots ethanolic extract may attenuate the toxicity of Meloxicam, leading us to deduce that Meloxicam induces hepatotoxicity and creates abnormalities in hematological and biochemical markers. ...
Faisal Alzahani1* and Mohammed Abu El-Magd2*
...higher expression of the apoptotic Bax gene and lower expression of the antiapoptotic Bcl2 gene than treatment with each alone. CAPE and CNPs co-treatment also inhibited global DNA methylation levels and downregulated the expression of DNA methylation-related genes (DNMT1 and Ube2e2) in HepG2 compared to cells treated with CAPE and CNPs each alone. These findings conclude that the cytotoxic impact of CAPE and CNPs combined t...
Estabraq Hayder Khayoon, Ammar Ahmed Abdulwahid*
...t. Curcumin’s anti-apoptotic, anti-inflammatory, and antioxidant properties have not been thoroughly examined in neurotoxicity given on by Acrylamide, nevertheless. We investigated whether Curcumin may protect against oxidative stress, apoptosis, and inflammation in neurons brought on by Acrylamide exposure. In conclusion acrylamide alters the neurobehaviors and oxidatively stressed animals .However Curcumin restored these parameters close to control va...
Peixia Yu1*, Lijun Bo2 and Xueyin Song1
...onin-I (cTnI). The total apoptotic cardiomyocytes, B cell lymphoma 2 (Bcl-2) and Bax protein and mRNA expression were detected in the myocardial ischemia (MI) region. The HR and the MAP of the Fen group exceeded that of the I/R group, while the LVDP and ±dp/dtmax were approximate to the basic values. The MDA concentrations and CK-MB values of the Fen group went down and the SOD activity went up when was compared with the I/R group. Whereas, cTnI concent...
SYEDA NADIA AHMAD1, MEHWISH NASIR1, AQSA NAUREEN1, SHAGUFTA ANDLEEB4, KAUSAR RAEES3, TAHIR ABBAS2, ASMA YOUNIS1 & KHAWAJA RAEES AHMAD*1
... revealed dose dependent apoptotic changes in follicular cells and chief cells of parathyroid. Chief cells MCSA showed slight variations. Nuclear MCSA of the chief cells showed significant (p<0.001) dose dependent decline. The present findings suggest that DZ is toxic to endocrine cells of thyroid and parathyroidat 5mgkg-1 or more, in mice, bringing about characteristic histopathological and micrometric changes in these tissues....
Taghreed Jabbar Humadai*, Bushra Ibrahim Al-Kaisei
...ear necrosis with number apoptotic hepatocyte, nuclear pyknosis with evidence of councilman body in liver tissue, The Immunohistochemistry study revealed an increase in the scoring of IL1 expression in liver that showed score (3) compared to control group, TNF alpha expression showed score (1) in liver tissue when compared to untreated control group . According to the findings of the current study, tetrodotoxin has adverse toxic effects on liver tissue of male...
Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*
...LC treatment had a lower apoptotic rate of blastocyst compared to the control group (35,8% vs 58,3%). In conclusion, LC could increase the maturation and blastocyst rates of oocytes and reduce apoptosis in Egyptian buffalo embryos....
Abdulla A. Albishtue1,3, Nurhusien Yimer1,5*, Md Zuki A. Zakaria2, Abd Wahid Haron1, Abdul Salam Babji4
...st that EBN’s anti-apoptotic, proliferative, and antioxidant properties lessen the neurotoxicity caused by lead acetate.
Keywords | LA, EBN, TAC, TBARS, Cerebellum, Cerebrum
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Aya Salah Eldin Mohamed1*, Gamal E. Shams1, Gihan G. Moustafa2, Reda M. Abd El-Aziz3
...ive stress, necrotic and apoptotic alterations, and protects against testicular damage caused by citalopram. Therefore, they might act as potential candidates to improve citalopram-induced testicular toxicity and damage in male rats.
Keywords: Citalopram, Ginseng, Vitamin D, Antioxidants
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Bingzhao Yan1, Guiping Chen2, Lulu Ding1, Ruxue Huang1, Wenjing Yu1 and Jicang Wang1*
...ncrease in the number of apoptotic cells in the testis of TUNEL. In addition, cadmium exposure significantly increased the mRNA and protein expression levels of PERK, CHOP, ATF4, eIF2α, GRP78, and the pro-apoptotic factor Bax, while the expression of the apoptosis inhibiting factor Bcl-2 was significantly decreased, and compared with the CdCl2 group, the CdCl2 + Que group showed significantly decreased gene expression ...
Salim Saifullah1* and Achyut Adhikari2
...y, anti-cancer, and anti-apoptotic properties. In this study, nepetin as yellowish powder was isolated from A. vulgaris extract through column chromatography. Structure of the compound was elucidated through various spectroscopic techniques such as Mass and NMR (1D and 2D)....
Mariam Ismail Ali1*, Rania Helmi Abdou1, Mary Ayad Sargious2, Omnia Mohamed Khattab2, Kawthar Abdelwahed Elhady1
... massive increase of pro-apoptotic Bax in the liver. Not only the potential beneficial role of bee venom on cadmium hepatotoxic effects but also nephrotoxic and neurotoxic effects and reducing hepatotoxicity caused by cadmium exposure via regulating, Bax, and NF-kB signaling pathways in rats by considering its possible antioxidant, anti-inflammatory, and anti-apoptotic effects. The results showed the nephrotoxic effect of ca...
Zainab A. Shehab1*, Assad H. Eissa2, Hind A.A. Alahmed1
...n important place in the apoptotic mechanism in kidney and liver tissue. A total of two groups including control and treated experimental groups (intraperitoneal injection of 5-FU (60 mgkg body weight) were formed in the study. After intraperitoneal injection of 5-FU on days 0, 5, 10, 15, all rats were scarified at day 21 and blood collected for hematological kidney and liver tissues taken from the rats and fixed in 10% formaldehyde solution for histological a...
