Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
...collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was co...
Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad
British Journal of Virology
...collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was co...
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
...collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was co...
El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter
Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
... has experienced endemic outbreaks of avian influenza viruses (AIV) of subtype H5N1 in poultry since 2008. Since then, these viruses continue to evolve. In 2006-2008, outbreaks were reported mostly during the winter season, but in 2009-2012 they occurred year round. H5N1 AIV succeeded in infecting not only domesticated birds but also many other avian species and mammals. Infections of humans were accompanied by mutations in ...
Simon Mwangi Kihu1*, George Chege Gitao1, Lily Caroline Bebora1, Njenga Munene John1, Gidraph Gachunga Wairire2, Ndichu Maingi1, Raphael Githaiga Wahome1, Davis Njuguna Karanja1, Julius Otieno Oyugi3, Ernest Lutomia3
...atic and devastating PPR outbreaks in Turkana County. In this context, an outbreak of PPRV was observed in Tukana County in 2011 and the current study detail the clinical, pathological and laboratory findings of this outbreak. The disease was clinically manifested by the depression, diarrhea, difficult breathing, muco-purulent ocular-nasal discharges with matted eyelids and encrusted nostrils and finally death. Clinical outcome, gross lesions and histological ...
Guoyi Li1, Haiyan Tong1, Jiansheng Chen2, Jinliang Wei2, Xiaoming Zhao2, Guoping Zhang2, Zixue Shi2*
E-mail | [email protected]
...ustralia, where periodic outbreaks take thousands of lives. With rapid globalisation and climatic shift, JEV has started to emerge in previously unaffected areas. Scientific evidences show that JEV would remain a global pathogen and might cause worldwide pandemics. Currently, more research is required to be applied to JEV pathogenesis and antivirus therapy. This review aims to reveal the exigency of developing a worldwide effort to acknowledge the importance o...
Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3
Email: [email protected]; [email protected]
...for its transmission and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is still a need for their evaluation under different conditions and in different ways. In the present study, representative disinfectants from chlorine and non-chlorine oxidizing agents have been evaluated for their virucidal ability against two distinct Egyptian subclades of H5N1 highly pathogenic avian influenza (HPAI); (A/...
Behzad Hussain1, 2, Abdullah Iqbal2, Muhammad Abubakar2*
...tiple sporadic cases and outbreaks have occurred in subsequent years and till today. CCHF outbreaks have a case fatality rate of up to 40%, which is alarming. It is a zoonotic disease and the virus is transmitted mainly to the humans through hard tick bite and direct contact with blood of the infected animal. The occurrence of CCHF outbreak in Pakistan is increased quite significantly near Eid-ul-Adha. Haemorrhages are the m...
Moustafa Kardjadj
...ponsible for most of the outbreaks. However, the rapid spread of SAT2 and other exotic FMDV lineages in Libya and Egypt demonstrated the need for a robust surveillance system to detect and respond effectively to exotic infections. Emergence and re-emergence of FMD virus genotypes/lineages has been detected necessitating frequent replacement of the vaccine strains. The present review summarizes the knowledge generated in epidemiology, di...
Vinayagamurthy Balamurugan*, Gurrappa Naidu Govindaraj and Habibur Rahman
... India as more number of outbreaks have occurred in the past and now occurring regularly, round the year and most frequently during the lean period throughout the country. In some Indian states viz. Andhra Pradesh, Karnataka, and Chhattisgar h the PPR outbreaks in sheep and goats have declined after implementing the strategic mass vaccination programme. The decreased number of outbreaks in...
Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar
...e occurrence of previous outbreaks of PPR virus due to persistency of virus in endemic regions....
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
... efforts to avoid future outbreaks in wild birds, as well as in commercial and backyard poultry.
...
Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki
...ASF in Nigeria, periodic outbreaks have continued to occur among domestic pigs with a few incidences in wild pigs. However, to investigate the risk of a tick-domestic pig transmission cycle in Nigeria, 3,288 serum samples were collected from domestic pigs from 10 states and analysed for anti-tick antibodies using the recombinant TSGP1 indirect ELISA. Of the samples analysed, 13.4% (442/3,288) showed moderate to high reactivity, suggestive of domestic pigs&rsqu...
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
... Pakistan causing severe outbreaks since 2005, the molecular epidemiology in mosquito (dengue vectors) is limited. Here, we investigated the vertical transmission of dengue virus (DENV) in the Aedes mosquitoes and its (DENV) transmission through other factors mainly tyres trade. DENV was detected in Aedes aegypti (57%) and Aedes albopictus (43%) adults (n=1050) and larvae (n=550). The samples were collected from four (Shuba Bazaar, Tarnab ...
Muhammad Abid1*, Tahir Yaqub2, Arslan Mehboob3 and Muhammad Zubair Shabbir4
... endemic in Pakistan and outbreaks are continuously being reported with high morbidity, drop in egg production and reduced weight gain. Besides economic losses to the poultry industry, these viruses pose a significant threat to public health. Owing to significant involvements of neuraminidase (NA) gene in virus pathobiology by mediating virus entry and release, the present study was conducted to determine the phylogenetic relationship of currently prevailing H...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
...out during the suspected outbreaks of PPR/FMD in sheep and goats in Hail. Saudi Arabia. During the outbreaks 271 (20.6%) died out of 648 (49.3%) of affected animals, the overall morbidity in goats (33.3%) was higher as compared to sheep (12.3%), with a case fatality rate in goats reached 74.69% in the 2nd outbreak. Affected animals exhibited fever, stomatitis, mucopurulent to bloody nasal/ocular discharges, watery to bloody ...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
...and prevention of future outbreaks.
...
Muhammad Abubakar,1,2,* Aamer Bin Zahur,1,3 Khalid Naeem1,3, Muhammad Azeem Khan1,3 and Subhan Qureshi4
...eighty-four (n = 84) PPR outbreaks were investigated during the study (2010 to 2013). The highest number of outbreaks was reported from Punjab province (n = 38) followed by Sindh (n = 21) and Khyber Pakhtunkhwa (KPK, n = 10). In 48 out of 84 outbreaks, disease occurred in goats only while 18 outbreaks affected sheep only and the remaining occurred in mix...
Muhammad Hidayat Rasool*, Abuzar Muhammad Afzal*, Abu Baker Siddique*, Waseem Akram**, Muhammad Saqalein*, Madiha Basit* and Muhammad Farooq Tahir***
ANTIVIRAL ACTIVITY OF VIRO CARE GZ-08 AGAINST NEWCASTLE DISEASE VIRUS IN POULTRY AND ITS IN-VITRO CYTOTOXICITY ASSAY
... necessary to prevent ND outbreaks. Natural resources have been exploited to obtain antiviral compounds in several latest studies. In this study, the antiviral activity of Viro Care GZ-08™ was checked up invitro, in-ovo and in-vivo. The cytotoxicity assay of the product was performed using Vero cell line. All the trials revealed that the stock solution and 1:2 dilution of GZ-08™ had some antiviral activity as well as were cytotoxic. As the concentr...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ion of vaccination, FAdV outbreaks have been reported and caused significant losses in poultry flocks throughout Pakistan in recent years. To identify the serotype and gain a better understanding of the genetic properties of the FAdV strains responsible for recent outbreaks, the sequence analysis of hexon gene of 10 isolates associated with IBH and HPS was determined. Sequence alignment and phylogenetic analysis based on dir...
Bo Wu1, Hui Zhang2, Kun Li2, Khalid Mehmood2,7, Yan Zhao1, Bin Jiang1, Chengjun Xue3, Muhammad Tariq Javed6, Fazul Nabi2, Zhaoqing Han5,* and Houqiang Luo4,*
...egions of China and many outbreaks are not reported. This study was undertaken to investigate the sero-prevalence and immunization program optimization of serotype O of FMD through ELISA and IHA in pigs in Zhejiang. A total of 368 serum samples were collected from June to July in 2016. Out of these samples, 23 (6.79%, 95% CI 4.4-9.9) pigs were found positive for FMD antibodies with the further distribution of 9.09% (95% CI 4.6–15.7), 6.90% (95% CI 2.6&nd...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
...Hail, Saudi Arabia where outbreaks are regularly reported. This study was carried out to analyze nucleoprotein (N) gene of PPRV from fatal infections in small ruminants. For this purpose, RNA was extracted from suspected animal samples (n=18) and were screened by real time-PCR. Additionally, to assess the phylogenomics, the N gene was amplified and sequenced from sheep and goats (n=12). Nucleotide identity among Hail strains was identified to be 96.2-100%, whi...
Waqas Ali* and Mudasser Habib
... causing majority of the outbreaks in the Pakistan during recent years. Moreover, only one residue of VP1 region was under positive selection pressure for serotype A (position 141) located in known critical antigenic region but as a whole protein was under negative selection pressure for serotype O, A and Asia 1. Therefore, under mass vaccination campaigns and drastic environmental conditions strains under negative selection has the ability to make escape muta...
Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1
...reduce the risk of PPMV1 outbreaks. ...
Ragia S. Mohamed1, Rania F. El Naggar2, Mamdouh. M. Hamoud1, Mohamed M. Hamoud3, Abdulrhman M. Gamal1, Samah E. Laban1, Shimaa A.E. Nasr1, Manal M. Zaki1, ElShaimaa Ismael1, Osama K. Zahran1*
...o the poultry flocks and outbreaks have occurred even in vaccinated flocks. Commercial Newcastle disease virus vaccines can provide various levels of protection against challenge with different NDV genotypes, raising the importance of the relationship between vaccines and field strains. The aim of the current work is to evaluate the effect of moisture percentage and pH on the persistence of velogenic NDV genotype VII in the poultry manure which considers a maj...
Muhammad Rehan1, Asim Aslam1, Muti-ur-Rehman Khan1, Muhammad Abid2, Shakeel Hussain3, Javeria Umber4, Ahsan Anjum1 and Altaf Hussain2*
...s and continuously cause outbreaks in commercial poultry throughout the world. Despite intensive vaccination, ND is endemic in Pakistan and locally known as Ranikhet. Wild birds are considered natural reservoirs of Newcastle disease virus (NDV) and some common resident or migratory wild birds are associated with outbreaks of ND in commercial poultry in Pakistan. Continuous isolation of new genotypes in Pakistan shows the evo...
Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3
...-align: justify;">Severe outbreaks of Infectious bursal disease virus (IBDV) were reported in Egypt despite vaccination. Therefore, this study was conducted to characterize infectious bursal disease (IBD) viruses circulating in Egypt during the period of 2017 - 2018. Sixteen pooled bursal tissue samples were collected from IBD suspected (9 Egyptian balady and 7 broiler) chicken flocks located in different localities at Sharkia governorate, Egypt. These sa...
Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan
...015-2018. Out of 588 NDV outbreaks 95 flocks were confirmed positive NDV by rRT-PCR using genotyping primers, with a prevalence rate of 16.2%. NDV outbreaks were recorded in 16 governorates, from total of18 investigated governorates and the recorded geo-prevalence of 89 %. Twenty-five samples were selected for further sequencing for the partial fusion protein. Phylogenetic analysis revealed that 20 samples are genotyped as v...
Irfan Irshad1, Asim Aslam1,*, Muhammad Yasin Tipu1, Kamran Ashraf2, Beenish Zahid3 and Abdul Wajid4
...in Pakistan and repeated outbreaks are continuously being reported with high mortality in poultry and non-poultry avian species. In this study, five AAvV-1s have been pathotyped and genetically characterized from vaccinated birds collected between 2013-14. A phylogenetic analysis revealed that all the isolates belonged to sub-genotype VIIi with high similarity of 97.9 to 99.8% with similar viruses in this clade. Analysis of the hemagglutinin (HA) gene sequence...
Khaled Kaboudi*
... Africa, where the first outbreaks were described since the end of 1990s, many features about epidemiology and genetic characteristics are still little known. This review summarized the epidemiological situation of H9N2 subtype in poultry in Algeria, Egypt, Libya, Morocco, Sudan and Tunisia. Human infection was reported in Egypt and serosurveys programs showed high positivity, particularly in poultry workers. Genetic analysis demonstrated that the most of H9N2...
Syed Maaz Nadeem1*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh1, Arfan Ahmad1* and Muhammad Anees2
...genes of CAV strain from outbreaks in broiler flock (n=45) were undertaken in the Punjab Province of Pakistan. The phylogenetic analysis clustered study strains of CAV in group II similar to those reported previously from China. These findings are helpful for an understanding of currently circulating strains of CAV; however, they require further analysis to intervene in necessary strategies to avoid future outbreaks in comme...
Detection and Phylogeny of Infectious Bursal Disease Virus (IBDV) during Field Outbreaks in Broilers
Umm-i-Habiba1,*, Ayesha Maqbool2, Muhammad Safdar3, Nida Zia1, Altaf Mehmood4, Muhammad Usman5, Mehwish Sharif6, Amanullah Khan7 and Sajid Umar8
...ndustry worldwide. Field outbreaks of IBD in 18 different poultry farms in the Chakwal district were confirmed by clinicopathologic examination and PCR. A total of 6 isolates of IBDV from these outbreaks were genetically characterized based on hyper-variable region of the VP2 gene. IBDV strains were grouped into two distinct clusters on the basis of nucleotide sequences of hyper-variable region of the VP2 gene. According to ...
Abdul Kabir¹, Amjad Hussian Mirani¹, Jam Kashif1, Shumaila Manzoor2, Abdullah Iqbal3*, Ihsan Ullah Khan4 and Muhammad Abubakar2
...tections showed that the outbreaks were going in study population and confirmed positive for PPRV by IcELISA and RT-PCR (N-gene). The study revealed a comparison of PPRV occurrence in the different production system of small ruminants and on-going disease outbreaks in the target area.
...
Shilong Chen1,2, Shifeng Xiao1,2, Shao Wang1,2, Fengqiang Lin1,2, Xiaoxia Cheng1,2, Xiaoli Zhu1,2, Dandan Jiang1,2, Shaoying Chen1,2* and Fusong Yu3*
...goose parvovirus causing outbreaks of short beak and dwarfism syndrome duckling in Chinese duck flocks. The aim of this review is to describe the current knowledge on SBDSV with an emphasis on epidemiology, clinical symptoms, laboratory diagnosis, genomic organization and pathogenesis. However, the pathogenesis mechanisms of cross-species transmission, retardation and beak atrophy are still needed to be elucidated. ...
Samia Afzal*,Sadia Zahid, Iram Amin, Muhammad Shahid and Muhammad Idrees
...ean have faced the major outbreaks of Chikungunya virus because of its travel associated febrile nature. An inexplicable paralyzing disease has trapped thousands of people in Karachi region of Pakistan and the symptomatology among suspected cases was compatible with Chikungunya fever. Thus Chikungunya outbreak was reported in Pakistan but actually the recent reports about the occurrence of Chikungunya outbreak in the southern region of Pakistan has been highly...
Muhammad Saeed Imran1, Asim Aslam1*, Muhammad Yasin2, Tahir Yaqoob2 and Beenish Zahid3
...fectious Bronchitis (IB) outbreaks are not uncommon in endemic countries. Therefore, the current study was conducted to investigate the efficacy of commercially available vaccines against each virus alone as well as co-infections with NDV and IBV in commercial poultry. For assessment of vaccine efficacy, commercial chickens were vaccinated with La Sota (against NDV) and Massachusetts (against IBV) vaccine preparations. After screening the protective titers in ...
Hafiz Muhammad Imran Javed* and Mushtaq Ahmed
...iginating from the field outbreaks for phylogenetic analysis, nucleotide and deduced amino acid sequence analyses, and a comparison of three-dimensional protein structures. The N gene ORF was 1578 nucleotide long with a single open reading frame encoding 526 amino acids. Similarly, the ORF of F gene was 1641 nucleotide long which encoded 547 amino acids. Upon phylogenetic analysis of the country isolates with the vaccine strains, it was revealed that isolates ...
Parakriti Gupta1, Kapil Goyal2 and Mini Pritam Singh3*
Hepatitis E Virus and Zoonosis
...veloping nations and the outbreaks occur mainly in the post monsoon season when the drinking water gets contaminated with sewage. However, the mode of acquisition of infection is different in developed nations with good sanitary conditions where the infection is a zoonosis. Though the virus has only one serotype, 8 genotypes have been documented. Out of these, HEV 1 and 2 have been reported from developing countries and are transmitted mainly through faeco-ora...
Mohammad Enamul Hoque Kayesh1,2*, Mohammad Tufazzal Hussan3, Md. Abul Hashem2,4, Mohammad Eliyas5 and A.K.M. Mostafa Anower1
Lumpy Skin Disease Virus Infection: An Emerging Threat to Cattle Health in Bangladesh
...hlight on impacts of LSD outbreaks in cattle industry of Bangladesh, and necessity of farmers’ awareness building and telemedicine service during the Coronavirus disease 2019 (COVID-19) outbreaks in controlling LSD....
Joseph Anejo-Okopi1*, Obinna Oragwa Arthur2, Ocheme Julius Okojokwu1, Sarah Joseph1, Geoffrey Chibueze1, Joshua Adetunji1, Joseph Ameh Okwori3, David Ochola Amanyi4, Otobo I. Ujah5 and Onyemocho Audu6
Seroprevalence of Rift Valley Fever Virus Infection among Slaughtered Ruminants in Jos, North-Central, Nigeria
...e the risk of unexpected outbreaks....
Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Mushtaq Ahmad1, Muhammad Saleem2, Ali Aziz2, Irfan Rasool2, Zeshan Ali3 and Muhammad Amin2
An Update on Biology, Extent of Damage and Effective Management Strategies of Chickpea Pod Borer (Helicoverpa armigera)
...s). In case of pod borer outbreaks, chemical insecticides remain as last option for farmers. However, management of chickpea pod borer through use of resistant cultivars, adopting recommended cultural practices and biological control measures have been found more effective, economical, sustainable and eco-friendly....
Shishir Sharma* and Laxmi Prasad Joshi
Current Insights on Stemphylium Blight of Lentil with its Management Strategies
...al in avoiding severe SB outbreaks. A comprehensive review article discusses the morphological as well as molecular dimension of pathogens, their effects, pathogenicity, integrated disease management approaches, and future views....
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
...ative agent of recent ND outbreaks in vaccinated broiler flocks in Menofia governorate, Egypt was found to belong to very virulent genotype VII. This strain was genetically identical to Egyptian genotype VII isolates isolated in the last period.
...
Md. Mukthar Mia1 and Mahamudul Hasan2*
...t owner to limit further outbreaks.
...
Hala K. Abdelmegeed 1, Eman M. Abohatab 1, Khattab,O. M. 1 , Salem, S.A.H. 1 , Arafa, A. 2, Nashwa M. Helmy 3
...e samples collected from outbreaks in period November 2013 –July 2014 the season Autumn and winter for forming an Epidemiological map for 10 governorates of the circulating strains during this period
...
Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3
...s enzootic form and many outbreaks had occurred since 1950 and
onwards. FMDV serotype (O) was the most prevalent until serotype (A) appeared in 2006 then
during April and May 2012, six outbreaks of FMD serotype (SAT 2) were reported in Egyptian
governorates. This study based on evaluation of vaccinated animals by detection of antibodies
against serotypes of FMDV (A),(O) and (SA...
Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2
...
Background: In Egypt, outbreaks of Newcastle disease (ND) have been occurring in vaccinated chickens with great economic losses. Objective: This work aimed for diagnosis and characterization of Newcastle Disease Virus (NDV) from infected broiler chickens in Egypt. Methods: Suspected virus isolates on embryonated chicken eggs was detected by Hemagglutination (HA) test and identified using hemagglutination inhibition (HI) test. F protein encoding gene of NDV ...
Muhammad G. Khodary1, Ayman H. El-Deeb2, Mohamed M. Emara2, Othman E. Othman1, Hussein A. Hussein2
...div>
disease (FMD) outbreaks among cattle and buffalo herds in Egypt. This study aimed to investigate the
contribution of Foot and Mouth Disease Virus (FMDV) and characterization of the circulating strains
associated with the outbreaks.
Methods: The collected samples of Oropharyngeal, oral epithelial tissue and/or vesicular fluids were
tested by real time Reverse transcri...
Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2
...sative agent of multiple outbreaks and
severe economic losses in the cattle population since its introduction in Egypt. Although vaccination is
practiced to control the disease, it is still endemic. Moreover, emergence of new strains is possible
through the international borders of the endemic countries. Consequently, there is a dire need for
routine molecular characterization of the circulating FMDV strains to e...
Ayman S. El-Habbaa1 and Mervat E. Radwan2
...t with repeated periodic outbreaks.
Objective: To investigate strain detection and genetic analysis of BEF virus among suspected cattle in
August 2017, Qualyubia, Egypt.
Methods: Partial sequence was generated after detection by reverse transcription-polymerase chain
reaction (RT-PCR) and subsequent gel purification of the amplified products of G gene of BEF virus.
Results: BEF virus was circulating i...
Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2
...ound: Lumpy skin disease outbreaks were observed in different governorates of Egypt
affecting cattle throughout the year and are still present. The progressive shift of the outbreak in Egypt
during 2018 causing severe losses in cattle population despite vaccination, which may incur some
debates on the efficacy of current live attenuated vaccines against CaPVs. The virus isolation,
identification, and vaccine or o...
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1
Junaid Ahmad1, Shahzeb Javed2*, Mehmood Khan1, Shadman1, Muhammad Farooq3*, Muhammad Saleem4, Rahila Afzal5, Muhammad Aasim6, Naqeeb Ullah7, Muhammad Noman Khan8, Dawood8 and Naila Ilyas9
...f health risks, illness, outbreaks, and even death. The goal of this study was to look at the students’ knowledge, attitudes, and practices on food safety and cleanliness at a different institution in KPK, Pakistan. A cross-sectional study was done among 1000 government and private university students in KPK, Pakistan, using a self-administered, pre-tested questionnaire with a total of 26 questions. Self-administered survey forms were used to collect dat...
Yonghua Liu*, Xianhua Li, Xiongfei Yan, Gang Li, Caiyun Luo and Ying He
...d there have been severe outbreaks of it in Hippophae rhamnoides plantations in North China. To study the effects of short-term high temperatures on its feeding, mating, longevity, and fecundity, T. vishnou gigantina were cultivated at 30, 35, and 40°C for 1, 2, and 4 h. The results showed that the different intensities and durations of the high temperatures had significant impacts on the moth’s survival and reproduction. With increased...
Amjad Ali Channa1, Mansoor Tariq1*, Zaheer Ahmed Nizamani1, Nazeer Hussain Kalhoro2
...c. Since last decade the outbreaks of AIVs have been increased globally including Pakistan. Therefore this study was conducted to determine the prevalence of avian influenza H5, H7 and H9 viruses in broilers at Karachi, Pakistan. A total of 1920 samples, including each of 960 blood samples and tracheal swabs were collected from broilers and tested through hemagglutination inhibition (HI), AIV-ELISA and RT-PCR. The HI test revealed a significant (P<0.01) dif...
Sameh Abdel-Moez Ahmed Amer1*, Mohamed Abdel-Aziz Kutkat1, Mohamed Mahmoud Abdel-Baki1, Asmaa Mahmoud Maatouq1, Omnia Mohamed Kutkat2, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1
...ion for birds against ND outbreaks, where expected to be due to genotype mis-matched vaccines to the rapidly evoluted NDV field strains. Herein, the present study investigated the prevalence and the phylography of currently epidemic genotypes of NDV. Out of 550 samples were collected during an active surveillance in 2020 and 2021 from different poultry farms in five Egyptian governorates, 100 pooled samples were tested for NDV identification. NDV replication a...
Martha Echioda Ogbole*, James Agbo Ameh, Samuel Mailafia, Olatunde Hamza Olabode and Bridget Jessica Adah
...haracterized by sporadic outbreaks of the disease occurring mostly during the dry season. It is hereby recommended that policy makers should timely provide prioritized budgetary allocation for the control of LF. In addition, sustained public enlightenment campaigns on strict hygienic practices and rodent control are also advocated....
Saima Parveen1, Altaf Mahmood2*, Ayesha Azad3, Sajid Umar4, Nosheen Shoukat6, Mirza Muhammad Arsalan Azam5, Qurat-Ul-Ain5 and Nausheen Akhtar Malik1
...tion. Infectious disease outbreaks adversely influence the production potential and consequently cause economic losses. Epidemiological data regarding magnitude of these disease outbreaks is of fundamental importance for planning of a comprehensive control strategy. With retrospective design, this study was conducted from January 2013 through December 2017 in order to assess the disease burden on broilers reared in different...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...nuous monitoring of IBDV outbreaks in vaccinated chickens and evaluation of the used vaccines are required.
Keywords | IBDV, Identification, Phylogenic tree, Native chickens, RT- PCR
...
Md. Taimur Islam1*, Mirza Mienur Meher2, Anas Bin Harun3 and Md. Golam Haider1
...ommon in the country and outbreaks of such diseases appear frequently. Therefore, an integrated approach including strict biosecurity, good farming practices and proper vaccination should be taken into consideration for the prevention of these respiratory diseases to ensure sustainable poultry production in Bangladesh....
Omnia Mohamed Khattab1*, Hala Kamel Abdelmegeed2, Mohamed Mahmoud Mashaly1, Mervat Hamdy1*, Naglaa Hagag1, Ayman Hamed3, Hanan Aly Fahmy3, Essam Ibrahim4, Momtaz Abdelhady Shahein2, Elsayyad Mohamed Ahmed2
...yptian isolates from the outbreaks and other EHV-4 strains available in databases proving wide distance with other EHV-8 and EHV-1. The study recommends rt-PCR as a screening test for the tentative diagnosis of EHV-4 in epidemiological studies and n-PCR as a sensitive differential test for the virus detection. The virological studies and molecular assays confirm the involvement of EHV-4 in equine abortion, suggests the possibility that latent virus reactivatio...
Gawhara Ahmed-Abdelmonem1*, Zeinab Aboezz2, Ahmed Habashi1, Saad Sharawi2
...volved in the latest FMD outbreaks in Egypt. Thirty-six samples (26 blood and 10 oral epithelial tissue samples) were obtained from suspected cattle in three Egyptian governorates during 2019-2020. The samples were screened for FMDV by means of real‑time RT‑PCR that showed nearly 86% (n=31) of the examined samples to be FMDV positive. Virus isolation was carried out on ten of the FMDV positive samples that were selected according to their cycle threshold (...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
Muhammad Tariq Navid1,2*, Mian Muhammad Awais2*, Muhammad Irfan Anwar2 and Masood Akhtar2
...recurrent episodes of AI outbreaks in a region. The viral shedding through oral and/or cloacal route may be the best way to disseminate infection towards the susceptible ones. Lastly, this study urges to vaccinate the rural poultry birds to prevent further spread of the AIVs that interrupt with commercial poultry production system and also with the community....
Abdul Kabir1, Muhammad Rasheed2, Hubdar Ali Kaleri5, Depeesh Kumar Bhuptani3, Mithan Kumar4, Raza Ali Mangi6, Abdul Wahid Solangi4, Sheva Dari1, Saqib Kakar4, Panah Munir4, Ekra Akbar4 and Rameez Raja Kaleri4,5*
Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1
...018-2020, causing severe outbreaks in duck farms in Egypt.
Keywords | Avian influenza, HPAI, H5N8, Ducks, Pathology, Egypt.
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Carla Meneguin Barbosa1*, Fabio Gregori2, Cairo Monteiro1, Thais Helena Martins Gamon1, Luciano Matsumiya Thomazelli1, Tânia de Freitas Raso2 and Edison Luiz Durigon1
... species, divided in two outbreaks, one year apart between 2016 and 2017, in a breeding farm in São Paulo State, Brazil. PSHv-1 was detected in 6 species (61 birds) through Nested-PCR, that tested negative for Influenza, Paramyxovirus, Flavivirus, and Adenovirus. Genetic sequencing and phylogenetic analysis support these results....
Sutawi*, Ahmad Wahyudi, Abdul Malik, Suyatno, Asmah Hidayati, Imbang D. Rahayu, Endang S. Hartatie
... the re-emergence of FMD outbreaks in Indonesia. First, the loss of the principle of maximum security. Indonesia’s success in freeing itself from FMD after 100 years is thanks to the implementation of maximum security policy by enacting a country-based system, which is to only import livestock and livestock products into the country from FMD-free countries. Second, the share of imports of boneless frozen beef/buffalo meat originating from India continued...
Shaimaa M. Ahmed1, Gehan M.A. Kassem1*, Fernando Pérez-Rodríguez2, Heba H.S. Abdel-Naeem1
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Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...en related to food borne outbreaks. On the basis of our findings, routine diagnosis of STEC and improvement of hygienic measures must be considered as a critical concern for public health....
Rogia SA Gomez*, Said H Mbaga
...sures to prevent disease outbreaks. Some challenges including technical support, finances, and negligence still remain and need to be addressed to reduce the incidence of disease outbreaks and indiscriminate use of drugs.
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Aqleem Abbas1, Mustansar Mubeen2, Waqar Younus1, Qaiser Shakeel3, Yasir Iftikhar2*, Sonum Bashir2, Muhammad Ahmad Zeshan2 and Azhar Hussain1
...lenges to prevent future outbreaks. Herein, we describe significant diseases and pests that are catastrophic to GB’s crops in the future. In addition, this review shows how diseases and pests impact the yields of GB’s crops....
Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2
...ect against repeated NDV outbreaks. Therefore, the aim of the this work is to prepare a secure, sterile and potent an oil inactivated vaccine from a recent local isolate genotype VII 1.1 “NDV-CH-EGY-GIZA-VVTNRC-2021” and evaluates its efficacy against commercially available NDV genotype II vaccines in broiler chickens. Eighty chickens were housed in four groups (A, B, C and D) of 20 birds per group. Group A has been received the experimentally prep...
Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A2
...aracterized by recurrent outbreaks in many countries where the virus is still endemic. This purposive sero-surviellance study was conducted to detect the presence of AI antibodies in chickens and AI appraisal of poultry farmers’ knowledge, attitude and practice in Abuja Municipal Area Council, Nigeria. One hundred (100) blood samples collected randomly post slaughter from chickens in live bird markets were screened and characterized using Agar Gel Immuno...
Liyun Chang*, Yazi Li, Yumei Cai and Chenghui Li
Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1
...jor foodborne Salmonella outbreaks, is a significant concern for public health and the agro-food sector. The use of bacteriophages, as a potential replacement, for antibiotics and as food antimicrobial agents to potentially target foodborne pathogens is gaining augmented attention. This study’s goal was to isolate and physiologically characterize lytic bacteriophages against Enteritidis, and to ascertain their efficacy in modulating bacterial population ...
Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.
Sharawil , S.S.A. and Abd El-Rahim2, I.H.A.•
...dentified from suspected outbreaks of PPR among sheep and goats in Qalyubia Province, Egypt at 2006. In this study six of this PPRV isolates were confirmed by reverse transcriptase-PCR (RT-PCR) using primer set for fusion protein (F) epitope, then cDNA were send to Institute of Animal Health Pirbright, England to analyze for their nucleotide sequences of this F protein gene and phylogenic analysis properties, by matching with other reference world record...
Arafa, A ; El-Kanawaty, Z; Hassan, M.K; Anwar, H.K and Mona M.Aly
...he introduction of early outbreaks of 2006. Viral diagnosis was based on direct detection of viral RNA by real time PCR. Two positive cases from turkey and Grocer duck were processed for viral isolation and characterization. The level of antibodies was detected in vaccinated birds by HI test and the results were discussed to evaluate the role of vaccination in controlling the disease in these valuable zoo birds....
Hussein, HA; sultan, HA. Al Deeb AH and AA. El-Sanousi
...-emerging of H5Nl severe outbreaks in vaccinaied chickens housed in poultry farms in Sharkia governorate in Egypt was observed starting from October 2007 and continued for 3 months until control measures have been taken in such farms. besides multiple vaccination during this period were applied using Reassortant H5N1 and 6 types Of H5N2 vaccines. In the present study, 9922 serum samples were collected from vaccinated chickens including some broiler breeders an...
M. E. Shawky and A. M. Daoud
...emergency control of FMD outbreaks. ...
Seham A. El-Zeedy1, Dalia A. Abd El-Moaty1, H. A. Hussein2 and M. A. Shalaby2
...n: justify;">Many recent outbreaks of Infectious bursal disease in commercial chicken flocks worldwide are due to the spread of very virulent IBDV. Two Egyptian isolates Of IBDV isolated from Giza (Giza/2000) and Qalyubia (Kal/2001) governorates were characterized by RT/PCR-RFLP assay of a 643 bp hyper variable region Of VP2 gene using BstNI, MboI, and SspI restriction enzymes. The presence of Sspl site in Giza/2000 identified it as vvIBDV while the absence of...
H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3
... investigation of severe outbreaks of infectious bursal disease (IBD) among vaccinated chicken flocks during the period 2002-2003 revealed that all IBDV outbreaks occurred at 18-33 days of age. 70% (21/30) of these outbreaks characterized by typical clinical signs and gross lesions. The mortality rates in these flocks ranged between 9.4-12.8 % (average 11.1%); 16.4-30 % (average 23.2...
M.R. Abd-El Wahab l, H.A. Hussein2, T.M. Asfourl and M.A. Shalaby2
...tion resulting in severe outbreaks. Also, the study demonstrates the continuous genetic diversity of BVDV and exalts the possibility that BVDV in camel may have a distinct group varied from those reported in bovine and ovine populations. ...
S. A. Khalil1; M. El-Sayed2; M. M. El-Fayomy2; H. Y. Hassan3 and A. Zaghawa3
...e disease caused several outbreaks during 2000, 2001 and lastly 2004. The persistence of immunity after natural infection is varied from 6 months to several years. Assessment or maternal antibodies in calves born to naturally infected dams is a matter of interest in order to determine the proper time of vaccination. Twenty-five pregnant Frisian cows in the last stage of pregnancy were naturally infected with BEFV during the Egyptian outbreak in summer 2004. Fo...
Aalaa S. Saad1*, Taghreed H. A. Ali2, Nayerah Alatfeehy3, Dalia Elmasry4
...f Listeria monocytogenes outbreaks caused by eating food is increasing worldwide, and people are increasingly looking for natural ways to stop the development of L. monocytogenes. In this context, propolis, a natural substance produced by bees, looks like an attractive option owing to its antibacterial action against many pathogens in food. The aim of this study is to investigate the effect of propolis extract (PE) and propolis nanoemulsion (PN) on L. monocyto...
Wahid Hussein El-Dabae*, Eman Shafeek Ibrahim, Eslam Gaber Sadek, Mai Mohamed Kandil
...an be used to control ND outbreaks in Egyptian poultry.
Keywords | Orthoavulavirus javaense, Attenuated vaccine, Genotype VII.1.1, Vaccination, Protection, Challenge
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