Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1
Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3*
...by histopathological and immunohistochemical analyses. Our results demonstrated that fusion protein VEGFR1(D1-D3)-Fc efficiently inhibited
the lung cancer growth in vivo. Although the aptamer RNV66 was not found to be as efficient in this study, the results were also not surprising as the aptamer was administered naked and at low dose. As these are only our preliminary studies, a detailed investigation is planned using multiple doses, chitosan nanoparti...
the lung cancer growth in vivo. Although the aptamer RNV66 was not found to be as efficient in this study, the results were also not surprising as the aptamer was administered naked and at low dose. As these are only our preliminary studies, a detailed investigation is planned using multiple doses, chitosan nanoparti...
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...erent colors examined by immunohistochemical analysis showed either weak (the black cashmere goat) or strong (the white cashmere goat) expression of the KIT protein. These findings suggested a relationship between mutations in KIT exon 13 and differential fur color in cashmere goats. These results lay the foundation for further research on exon 13 of the KIT gene and color regulation in cashmere goats.
...
Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*
...expression was weak. The immunohistochemical results was consistent with the molecular expression of AR mRNA, the positive reaction in the infundibulum, isthmus and uterus is more intense, followed by the magnum, the vaginal reaction is weak. Positive responses in the sections were mainly focused on luminal epithelium, glands and vascular epithelial cells. This study revealed the molecular expression of AR and the distribution of intracellular proteins in the ...
Zartasha Anjum1*, Rakhshindah Bajwa2 and Munazza Hassan3
...conducted to examine the immunohistochemical expression of HMG-CoAR in 75 cases of breast carcinoma. Study was carried out in postgraduate medical institute, Lahore. The frequency and percentage were calculated for histological grade of the tumor and expression of HMG-CoAR, ER and PR. Fischer exact test was applied to determine the association between HMG-CoAR and ER/PR expression.
Results: HMG CoAR, ER and PR were found positive in 69...
Shaher Bano1, Sarah Ghafoor1,* and Nadia Naseem2
...the control animals. The immunohistochemical expression of TGF-α in ductal epithelium of both salivary glands was decreased in experimental group sacrificed at 5 weeks. However no significant change was observed in TGF-α expression in ductal epithelium of salivary glands in rats sacrificed at 9 weeks.T3 has a temporal effect on TGF-α expression during early postnatal rat salivary gland development.
...
Shaher Bano1, Nadia Naseem2 and Sarah Ghafoor1,*
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...cular, pathological, and immunohistochemical analysis to detect this pathogen in pet dogs. Molecular methods, combining three types of PCR assays, identified two strains isolated from two pet dogs as Brucella canis. Histopathological changes revealed extensive inflammation and necrosis in the liver, lung, spleen, kidney, testicle, and lymph nodes, among which changes in the spleen were the most serious. Immunohistochemistry results demonstrated the dete...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2, Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1, Yuriy Kharkevych1 and Magdalena Gryzinska4*
...passages was examined by immunohistochemical method using monoclonal antibodies. Determination the index of proliferationdogs adipose-derived stem cells (DADSCs) on IVth and Xth passages. Established that DADSCs contains multipotent stem cells, that are characterized by an almost homogenous fibroblast-like cells оn the IVth and Хth passages. It was difined, that fibroblast-like cells оn the IVth and Х<...
Fei Wang1, Xianlai Zhang1, Youbao Zhong2, Xiaoen Tang3, Xiaofen Hu1,
ShanShan Yang1, Shengwei Zhong1, Zuohong Zhou1, Jin Liu1, Xu Yuan1
and Yong Li1*
... consistent with that of immunohistochemical detection. It is indicated VIP probably participate in the functional regulation of pigeon intestine as a key role and mediate the neuroendocrine and mucosal immune system as an information molecule due to the high expression of VIP. It will provide the basis for clarifying the biological function of VIP in pigeons.
... 
Safaa M. Mohammed*, Shahira A. Abdelwahab**,Fetaih,H⃰ ⃰ ,Neveen,R⃰ ,Abdel satar ,A⃰ and Mohamed S. Elshahidy**
...,histopathologically and immunohistochemically. Turkey pox virus was isolated on CAM of embryonated chicken eggs and identified by agar gel precipitation test and polymerase chain reaction .Two main types of TKPV infection were observed in examined flocks ,cutaneous and diphtheritic forms. Morbidity rate ranged from 5-15%with no mortalities .75% of examined serum reacted positive to TKPV .All TKPV isolates were amplified using specific P4b primers and visualiz...
Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1
...
antigens by immunohistochemically in thymus and bone marrow tissues of SPF chicks infected with
CAV.
Methods: In this study, 30 layer farms of age 40 to 100-days-old from different governorates
(Alexandria, Gharbia, Giza and Qalubia) were examined for CAV with real-time PCR and
histopathological examination. The positive samples were prepared and inoculated in SPF chicks' oneday-
old then...
HuaBin Zhang1,2, YaSen Li1, Tao Pan1, Peng Yan1, En Li1, Hui Xue1 and XiaoBing Wu1,*
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...velopment was studied by immunohistochemical staining, immunofluorescence, QRT-PCR and western blot. Immunohistochemical staining results showed that Gas6 was expressed in the oocytes, especially the nucleus, from the primary to preovulatory stages. The further immunofluorescence results showed that Gas6 was expressed thoughout the in vitro matured porcine oocytes, and the staining signal decreased in the first...
Yu Yang1,2, Shengqiang Ye1, Lixia Wang1, Xing Chen1, Yunguo Qian1 and Ping Gong1,*
...of adult mule duck using immunohistochemical staining method. The result showed that DMRT1 was mainly expressed in spermatogonia nucleus of seminiferous tubules and had no expression in Leydig cells. It was indicated that the DMRT1 expression was positively correlated with the testes development of adult mule duck.
...
Ho-Seong Cho1 and Yeonsu Oh2,*
Zekeriya Özüdoğru1, Hülya Kara2*, Adem Kara3, Derviş Özdemir3 and Hülya Balkaya2
Keqiang Wei1*, Yue Wei2 and Changxia Song1
...this work, we applied an immunohistochemical method to investigate their cellular localization and expression in hepatopancreas of red swamp crayfish (Procambarus clarkii), a commercially important aquaculture species in China. Using polyclonal antibodies, the strong staining of these proteins in hepatopancreatic cells was observed. The synchronous expression of p38 and its phosphorylated form (p-p38) was mainly present in B- and R- cells, and their MOD values...
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
...chemistry were done. The immunohistochemical test exposed the immunophenotype positive for CD79a. Finally, B-cell (CD79a) polycentric lymphoma was confirmed. This case report introduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practice in China, which can provide a reference for veterinarians in clinical diagnosis....
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
...chemistry were done. The immunohistochemical test exposed the immunophenotype positive for CD79a. Finally, B-cell (CD79a) polycentric lymphoma was confirmed. This case report introduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practice in China, which can provide a reference for veterinarians in clinical diagnosis....
Jing Fu
... groups were detected by immunohistochemical staining. SATB2 in mucinous ovarian tumors had a diagnostic sensitivity of 82%, a specificity of 78%, and an overall accuracy rate of 82%. CK20 in mucinous ovarian tumors had a diagnostic sensitivity of 94%, a specificity of 59%, and an overall accuracy rate of 85%. SATB2/CK20 in mucinous ovarian tumors has a diagnostic sensitivity of 65%, a specificity of 99%, and an overall accuracy rate of 89%. Compared with SATB...
Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1
Bnar Shahab Hamad1, Bushra Hussain Shnawa1*, Rafal Abdulrazaq Alrawi2
...stopathologically and by immunohistochemical markers staining. The infected livers with hydatid cysts from the mice were processed for routine paraffin block technique. The sections of infected livers that were embedded in paraffin underwent immunohistochemistry. Several immunolabeled liver sections with anti-CD3, CD4, CD8, CD20, CD68, and caspase-3 were examined under a light microscope. The histological alterations caused by the hydatid cyst were cell degene...
Jie Li, Gaofu Wang, Xiaoyan Sun, Lin Fu, Peng Zhou* and Hangxing Ren*
...CR, western blotting and immunohistochemical analysis. This study provides a theoretical basis for further clarifying the biological function of the KITL gene in goat....
Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1
...e different tissues. The immunohistochemical characterization of viral antigen revealed direct relation between viral residence in tissue and developed pathology. The present study confirmed that H5N8 HPAI clade 2.3.4.4b, became a predominant strain during the period 2018-2020, causing severe outbreaks in duck farms in Egypt.
Keywords | Avian influenza, HPAI, H5N8, Ducks, Pathology, Egypt.
...
Magda A. Eldomiaty1,2, Manal E. Elsawaf2, Soad S. Ali3, Heba El-Sayed Mostafa4,5*
...tron microscope, and for immunohistochemical expression. Rats from group III showed statistically increased corticosterone level and increased cortical thickness compared to other groups. Voluntary exercise improved these measures in rats of group IV. Histological disorganization of cortical cells was observed in group III with manifest large cytoplasmic vacuoles. Enormous increase in the number of both apoptotic and proliferative cells was seen with significa...
Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1
Shaimaa A.Tawfik2,3, EL S.T. Awad1*, Hoda O.Abu Bakr1, Amira M.Gamal-Eldeen4, Esmat Ashour2, Ismail M.Ahmed1
...athological examination, immunohistochemical analysis of CYP3A4, MRP-1, and c-MYC proteins and expression of miRNAs were assayed. Results: Findings indicated that CSO treatment led to a suppression in hepatotoxicity, nephrotoxicity and cardiotoxicity induced by DOX in male albino rats evidenced by a significant enhancement in the activity of ALT, GGT, LDH, AST, also creatinine and uric acid. CSO induced antioxidant activity GSH, CAT and inhibited MDA. His...
Thekra Fadel Saleh, Omar Younis Altaey*
...measures were taken, and immunohistochemical analysis was performed to evaluate B lymphocyte distribution. The study found that in Muscovy ducks, the left caecum was longer and heavier than the right caecum, and the proximal portion of both caeca was wider and had thicker walls compared to the middle and distal portions. Histologically, the length of villi and depth of crypts were greater in the proximal portion compared to the middle and distal portions. Hist...
Ibrahim Elmaghraby*, Abdel-Baset I. El-Mashad, Shawky A. Moustafa, Aziza A. Amin
...further histological and immunohistochemical examination.Results: Interestingly, our results diagnosed 15 cases of sex cord-stromal tumors (8.30 %) in the ovary of dromedary camels included; adult granulosa cell tumor (0.55 %), interstitial cell tumor (0.55 %), thecoma or fibrothecoma (1.10 %), steroid cell tumor-NOS (0.55 %), and granulosa-theca cell tumor (5.55 %). Specifically, all cases had distinctive histomorphological appearance characteristics for beni...
Muhammad Nasir Bhaya1,2* and Hikmet Keles2
...in the CP+EA75 group. In immunohistochemical sections, 8-hydroxy-2-deoxyguanosine (8-OHdG) and hypoxia-inducible factor-1 alpha (HIF-1α) revealed less positivity in EA received groups but B-cell lymphoma-2 (Bcl-2) revealed more positivity as compared to CP. Both histopathological and immunohistochemical findings revealed that EA has a protective effect against CP-induced renal damage in rats....
Zhipeng Song1,2, Jialiang Xin1,3, Xiaoli Wei1,2, Abula Zulipiya1,3, Kadier Kedireya1,2 and Xinmin Mao1,3*
...e immunofluorescence and immunohistochemical staining. The mRNA level of VEGF was detected by RT-qPCR. In the in vitro experiments, cell viability was detected by CCK-8 reagent. The expression of related proteins was detected by western blot. We found that marein can effectively reduce triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL) and blood glucose concentration (BGC) in db/db mice (P < 0.05). The results showed that marein could...
Ali Mosa Rashid Al-Yasari1, Zahid I. Mohammed2, Haider S. Almnehlawi3,4, Ali F Bargooth5, Nawar Jasim Alsalih1, Huda F. Hasan6*, Mohenned A Alsaadawi1
...ssues were collected for immunohistochemical identification of pancreatic cells that produce insulin and glucagon. Based on our results, none of the research parameters significantly differed across the groups on day 0. Days 1 through 28 showed that STZ-Diabetes group had the uppermost blood glucose level with lowermost blood insulin among the study groups as a result of the induction of diabetes. Comparing the STZ group to the control and camels’ milk g...
Shafi Muhammad1, Bibi Nazia Murtaza2, Aftab Ahmad1, Muhammad Shafiq3, Nurul Kabir4 and Hamid Ali1*
Usama Mahalel1, Barakat M. Alrashdi1, Ibrahim Abdel-Farid1, Sabry El-Naggar2, Mohamed Hassan3, Hassan Elgebaly1 and Diaa Massoud1,4*
Lijiao Wang1, Haibin Chen2*, Hongyan Yu1, Zexian Fu3 and Jianjun Zhao2*
...lected. Western blot and immunohistochemical methods were used to detect the expression of EPAS-1 in the three groups. The expression of EPAS-1 and the clinicopathology of RCC were further analysed. To examine the relationship between these characteristics, a receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of EPAS-1 expression for lymph node metastasis in RCC. Compared with the adjacent tissue, the expression of EPAS-1 i...
Rondius Solfaine1, Faisal Fikri2, Salipudin Tasil Maslamama3, Muhammad Thohawi Elziyad Purnama4, Iwan Sahrial Hamid5*
...mples were collected for immunohistochemical analysis. The findings revealed that CCoV vaccine induction in the treatment group resulted in duodenal mucosa haemorrhage, necrosis, and severe inflammation. Conversely, in the group treated with Lactobacillus acidophilus, tissue damage was inhibited, demonstrating the protective effect of the probiotic. Lactobacillus acidophilus effectively mitigated lung, liver, and duodenal damage by reducing the expressions of ...
Taghreed Jabbar Humadai*, Bushra Ibrahim Al-Kaisei
Yanhong Zhao, Bo Guo, Lin Fu, Biao Liu and Jing Xue*
... typing and pathological immunohistochemical (PI) of lymphoma cell leukemia (LCL), and then provide scientific data for clinical typing of lymph node cell leukemia. The BMFC typing and PI data of 86 patients with LCL admitted to our hospital from January 2018 to January 2022 were retrospectively analyzed to compare the BMFC typing and PI expression, and investigate the differences in PI results among patients with different BMFC typing. There were significant ...
Xiaodong Lin*, Guangyao Wu and Fu Zhang
...elected as controls. The immunohistochemically results demonstrated that occludin positive cells were mainly expressed in the cell membrane and cytoplasm of epithelial cells and glandular cells, with brownish yellow staining particles. The expression of occludin positive cells in the observation group was significantly lower than that in the control group (P<0.05). β - defensin positive cells were mainly expressed in the cytoplasm of intestinal epithel...
Rabab Abd Alameer Naser1*, Sameer Ahmed Abid Al-Redah2, Enas S. Ahmed3, Fatimah S. Zghair2, Ali Ibrahim Ali Al-Ezzy4
...opic, histochemical, and immunohistochemical levels. Seven male turkeys and ostriches were obtained from Diyala province during the summer (July-September). Euthanasia was performed using anesthesia with Xylazine and Ketamine. Esophageal sections (cranial, middle, and caudal) were collected for microscopic, immunohistochemical, and biochemical analysis. Compared to turkeys, ostriches lacked a distinct crop (second esophageal...
Hong Chen1,2, Jiawei Liu1,2, Chuan Lin1,2, Hao Lv1,2, Jiyun Zhang1,2, Xiaodong Jia1,2, Qinghua Gao1,2 and Chunmei Han1,2*
...(P < 0.05); the brown immunohistochemical reaction products were concentrated in the mesenchymal cell membranes and intercellular matrix of healthy and regenerated antlers. Our results suggested that the repair process of antler damage after top pruning treatment mainly promotes the proliferation and differentiation of antler chondrocytes, osteoblasts, and T lymphocytes through biological signals such as WNT, IHH, and IL2RA to ensure the development of antl...
Adham Omar Mohamad Sallam1*, Ashraf Abd El-Hakem Ahmed El-Komy1, Enas Abdulrahman Hasan Farag2, Samar Saber Ibrahim3
... 0.05). Histological and immunohistochemical analysis confirmed these findings, showing reduced tissue damage and apoptosis. In conclusion, the obtained results suggest that GSE protects against Meloxicam-induced hepato-renal toxicity, with a marked reduction in oxidative stress and inflammation, supporting its potential therapeutic role.
Keywords: Meloxicam, GSE, SOD, CAT, MDA, Histopathological. Immunoh...
Yonghong Ma1, Jinlan Ma2, Qifu Zhang3, Huajie Zou1 and Shenghua Ma1*
...ablishment, HE staining, immunohistochemical staining and real-time quantitative PCR were performed to compare the groups. The situation after the successful model creation; observe the two groups of TGF-β1, P53, microRNA103 and microRNA105 mRNA expression determination and TGF-β1 and P53 protein expression levels. The success rate of the experimental rat model was 100%; the expression levels of TGF-β1, P53, and microRNA105 mRNA in the experimen...
Sherine Abbas1, Hadeer M. Shosha2, Hala M. Ebaid2, Heba Nageh Gad El-Hak2, Heba M. A. Abdelrazek3*
...or histopathological and immunohistochemical expression. The results indicated there was significant tolerance of female offspring than male as indicated by ALT, urea and creatinine. A notable alteration in the biochemical parameters of blood serum (ALT, AST, albumin, total protein urea, uric acid and creatinine), as well as in the histopathological changes and immunohistochemical expression of tumor necrosis factor (TNF) in...
Sherine Abbas1, Heba M.A. Abdelrazek2*, Eman M. Abouelhassan3, Nayrouz A. Attia4, Haneen M. Abdelnabi4, Seif El-Eslam E. Salah4, Abdelrahman M. Zaki4, Mohamed A. Abo- Zaid4, Nadia A. El-Fahla5
...by increased (P<0.05) immunohistochemical expression of IL-6, NFKB and TNF-α in addition to induced spleen and thymus histopathological alterations. The study concluded that methanolic neem leaves extract has a cytotoxic effect that affects the histo-architecture of lymphoid organs, promoted inflammatory markers and the mononuclear cells’ DNA damage that seemed to be as a result of neem extract oxidative stress. This effect could be implemented ...
Zainab A. Shehab1*, Assad H. Eissa2, Hind A.A. Alahmed1
...ion for histological and immunohistochemical analyses. The results showed that 60 mg/kg of 5-FU drug significantly decreased RBCs and WBCs count in all rats in comparison , furthermore results referred that HB concentration was significantly decreased in 5-FU group compared to the control group The histopathological examinations revealed that while the liver and kidney tissue had a normal structure in the control group, glomerular degeneration increase in the ...
