Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...ahexaenoic acid (DHA) on frozen-thawed quality of bull semen. Twenty-four ejaculates (8 from each bull) were collected from three bulls. Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored into liquid nitrogen for 24 h...
Ibrar Muhammad Khan1, Rifat Ullah Khan1,*, Muhammad Subhan Qureshi2, Tahir Usman2, Adnan Khan1, Zakir Ullah1 and Hamid Rehman1
... 0.28%) were observed in frozen semen in cross-bred bulls, which showed significant (P < 0.01) differences with the other cattle bulls. The results imply that Sahiwal bulls showed the best semen qualitative characteristics in fresh and frozen semen conditions followed by Holstein Friesian bulls. Moreover, the semen quality declined in the crossbred bull.
...
Muhammad Waheed Mushtaq1,*, Jamil Anwar2, Omara Naeem3,Farah Kanwal1 and Waheed-uz-Zaman1
... various sections of the frozen mass and bacterial colonies were counted after proper Gram staining. It was found that more than 90% bacteria were separated along with freezing front and were concentrated in the centre in the case of radial freezing and at the bottom in case of vertical freezing.
...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
... extender on chilled and frozen thawed quality of Kundhi buffalo bull semen. After collection ejaculate was brought in Semen Production Unit. Semen was evaluated as fresh for volume, colour, motility, morphology, live dead ratio, membrane integrity and sperm concentration, post chilling for motility, morphology, live dead ratio, membrane integrity and frozen thawed for motility, morphology, live dead ratio and membrane integ...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
...itamin E) could increase frozen semen quality and the ability of antioxidant. Nevertheless, the effect on compatibility of the two antioxidants was better than single addition.
...
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
... various sections of the frozen mass and concentration of enzymes was determined. It was found that more than ~85% mass of enzymes, migrated along with freezing front and concentrated in the centre in case of radial freezing. To examine the effect of unidirectional freezing over extracted enzymes, the working activity of separated enzymes was checked by performing desizing test for alpha-amylase and biopolishing test for cellulase. The noteworthy results of de...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...etate) on the quality of frozen-thawed bovine spermatozoa. In this study, ten Polish Holstein-Friesian bulls with an average age of 3.5 ± 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8&...
Pankaj Kumar Jha1,2*, M. Golam Shahi Alam1 and Farida Yeasmin Bari1
Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes
...ris based) and number of frozen-thawed ram sperm (100 × 106 and 50 × 106 sperm dose) on pregnancy and lambing rate following laparoscopic artificial insemination (LAP-AI) in indigenous ewes in Bangladesh were studied. A total of 78 non- pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; G1 (n = 18; commercial Triladyl®/ 100 &time...
Alamaary Mohaammed Saad1,3, Abd Wahid Haron1*, Mohamed Ali2,
Mark Wen Han Hiew1 and Lawan Adamu1
...fect the OS level in the frozen semen. This study was to determine the addition of different concentrations of antioxidants to evaluate oxidative stress and fertility of frozen semen in horses. Seven stallion were used for semen collection in this study. A total of twenty ejaculates was collected from three healthy stallions twice a week for the experiments 1 and 2. All ejaculates were divided into two parts of ten ejaculate...
Maha I. El Zaafarany1, WafaaM. Badawy2, Eman M. El Salakh3
... cancer.Fifty four fresh frozen breast cancers samples were analyzed. Samples were tested for HPV by PCR, and products were sequenced. Findings were correlated with clinical and pathological characteristics.The HPV DNA prevalence in the breast cancer samples was 28 % (15/54). Sequence analysis in a subgroup of cases indicates the prevalence of high risk HPV16. In conclusion, the present study demonstrated for the presence of HPV in Egypt in a proportion of the...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...ed quality indicators of frozen buffalo bull’s spermatozoa. Semen samples were obtained from the bulls for three weeks through artificial vagina. Egg yolk was used as an extender, in which different concentrations of GT extract (0.0%, 0.50%, 1.0%, 1.5%, 2.0% and 2.5%) were added. Semen quality indicators studied were the percentage of motility, survivability, plasma membrane integrity and acrosome integrity of frozen b...
Amir Z. Allam, Hayam A. Mansour, Nermeen M.L. Malak, Heba H.S. Abdel-Naeem*
...cken meat patties during frozen storage at –18 °C for 12 weeks by enumeration of aerobic plate count (APC), psychrotrophic, Enterobacteriaceae, and lactic acid bacterial (LAB) counts. The results revealed that the sole effect of T, CL, and O oleoresins caused non-significant reductions in all investigated bacterial counts. Among the treated groups (T, CL, or O alone), it was found that O oleoresin achieved the highest reduction rate, followed by CL w...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...0.5mL straws for 4 h and frozen in liquid nitrogen at -196 °C. The parameters evaluated were percentage motility, plasma membrane integrity (HOST assay), acrosomal integrity (NAR), viability (Live/Dead), DNA integrity (Acridine orange assay) and oxidative stress (TBARS assay). Five straws from each Gallic acid group were thawed individually in water bath at 37°C for 30 seconds. The addition of 15 µM GA to semen extender improved marginally the bu...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
...TCA extender ameliorated frozen-thawed quality of beetal buck spermatozoa.
...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2
... was proceeded until the frozen semen. Parameters were motility, motility progressive, kinematics sperms, viability, abnormality membrane integrity, and acrosome integrity. The Bali-polled bull semen was analyzed by using CASA with the Sperms Vision Version 3.7.5 program. The results in this study showed that sperms motility in Bali-horned bull was significantly lower (P<0.01) than Bali-polled bull, Brahman, Limousine, and Simmental. The sperms viability in...
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
...ates, and prepared to be frozen. In 1st replicate, semen was extended in Tris egg yolk (TEY) is contained synthetic sources of energy (fructose), antibiotics (penicillin and streptomycin), and antioxidants (vitamin E), which served as control extender (E0). However, 2nd, 3rd, 4thand 5th replicates were serviced as trial extenders as E1, E2, E3, and E4, which supplied 0.50, 1.00, 1.50, and 2.00 % of BBE as natural sources of energy, antibiotic and antioxidant, ...
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...acteristics of fresh and frozen semen quality of Aceh cattle at different ages. Three Aceh bulls were involved in this study. The semen was collected from each bull aged 6 to 10 years and the fresh and frozen semen quality was evaluated directly. The results showed that the bull age significantly affects semen volume, pH, sperm concentration, total sperm, total motile sperm, and frozen sem...
Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1*
.... Moreover, the CLs were frozen at -80 °C for NO estimation in CLs homogenate, RNA isolation and gene expression. Blood samples were obtained for P4 serum levels estimation. Results showed a significant rise of TNFα mRNA during late stage of CL and increase the expression of CASP3, FASLG and AGTR2 mRNAs during mid-stage of CL. Furthermore, NOS2 mRNA was raised during early stage of CL. Surprisingly, NO in CL homogenate was raised through early and la...
Kirana D.D. Adiputra1*, Tulus Maulana3 , Ekayanti M. Kaiin3, H. Hasbi2, Herry Sonjaya2
...ne the quality fresh and frozen semen of Bali and Simmental bull in the Technical Implementation Unit of Regional Artificial Insemination Center at Pucak, South Sulawesi. Two Bali bulls and two Simmental bulls aged 5-6 were used. The parameters observed in the fresh semen were volume, pH, colour, motility, viability, abnormality, intact plasma membrane, and concentration. Meanwhile, parameters observed in frozen semen were m...
Amal G. Abdelrahman1, Nabil A. Yassien1, Hussein M.H. Mohamed1, Khaled S. Tolba2, Heba H.S. Abdel-Naeem1*
... All groups were kept in frozen storage at ‒18 °C for 3 months and subjected to proximate chemical analysis, measurement of deterioration criteria, and sensory quality. The results revealed that all enzyme-treated samples significantly increase protein content and significantly decrease the fat content, pH, and thiobarbituric acid value with a significant improvement in raw and cooked sensory attributes as compared to their counterpart control samples. S...
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...to depict the quality of frozen-thawed bull sperm extended in Tris-buffered extender (TBE) supplemented with docosahexaenoic acid (DHA). Electro ejaculator was used to collect the semen and ejaculates having ≥ 20 × 109 spermatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplemen...
Sutawi*, Ahmad Wahyudi, Abdul Malik, Suyatno, Asmah Hidayati, Imbang D. Rahayu, Endang S. Hartatie
...e of imports of boneless frozen beef/buffalo meat originating from India continued to increase from 33.8% in 2016 to 51.9% in 2020. Third, economic bioterrorism, an act of terror using biological agents that infects Indonesia’s cattle farming agribusiness. FMD outbreaks have a negative impact on cattle agribusiness from upstream to downstream: (1) the cattle population will shrink, (2) imports of cattle and beef will increase, (3) beef consumption will d...
Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*
..., the quality of unsexed frozen semen was higher than sexed frozen semen both in sexed X sperm and sexed Y sperm. While the proportion of sperm in sexed X sperm had a proportion of sperm X higher than sperm Y, indicating that Percoll Density Gradient Centrifugation can separate with an accuracy of more than 80% based on the head measurement.
Keywords | Sexed semen, Percoll gradient, Motility, A...
Yusmarini Yusuf*, Usman Pato*, Shanti Fitriani, Emma Riftyan, Evy Rossi, Diky Arma Fauzi, Ghina Ismadiah, Miftahul Hidayah, Windy Sabiliani
...h standards during early frozen storage. A completely randomized design with four treatments and four replications was used in this experiment to create 16 experimental units. The treatments in this study were the addition of several types of preservatives with different concentrations, namely without the addition of preservatives (P1), the addition of 0.3% sodium nitrite (P2), 2.5% chitosan (P3), and 0.6% bacteriocin (P4). All samples were stored at freezing ...
I Nyoman Sulabda1, Anak Agung Gde Oka Dharmayudha2, I Wayan Nico Fajar Gunawan3, Dwi Fortuna Hashilonda4, Luh Gde Setyawati5, I Ketut Puja6*
...s study aimed to provide frozen semen of Kintamani dogs for rapid improvement of the quality and population of Kintamani dogs with the specific target of evaluating the fertilization capacity of frozen semen of Kintamani dogs using the artificial insemination method. Eight bitches were used in this experiment. The dog was in good health. The time of artificial insemination was performed 11 days after the onset of proestrus. ...
Tarique Ahmed Khokhar1*, Atta Hussain Shah1, Muneer Ahmed Jamali1 and Asghar Ali Kamboh2
...racteristics of chilled, frozen and thawed meat against fresh meat. Proximate composition such moisture, protein, fat, ash and glycogen decreased with increasing storage period of chilled, frozen and thawed buffen, chevon and chicken meat samples. Nutritive values of chilled, frozen and thawed buffen sample also decreased with increasing storage period....
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
... in 0.25 ml straw, and refrozen into liquid nitrogen vapor about 5 cm above the liquid nitrogen surface for 10 minutes. Freezing was run by immersing the forage in liquid nitrogen for 24 hours before the post-thawing examination. Results showed that spermatozoa diluted with egg yolk citrate supplemented with 3% lycopene (P3) had better quality than that found in the other four treatments, namely motility before freezing (70.86%) and post thawing (53.00%); viab...
Achmad Firman1*, Sondi Kuswaryan1, Lilis Nurlina1, Muhamad Hasan Hadiana1, Marina Sulistyati1, Unang Yunasaf1, Dwi Cipto Budinuryanto2, Iman Trisman3
...s, such as the number of frozen semen distribution, the number of cow acceptors, the number of cow pregnancies, the service per conception rate, the number of calf’s birth, and the economic impact. Data collected by Directorate General of Livestock and Health (DGLH) was for five years. The Key Performance Indicators utilized to analyze the program performance. The incremental capital-output ratio (ICOR) model was used to determine the economic valuation....
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...ed in French straws, and frozen in liquid nitrogen at -196 °C. Evaluation of semen was achieved pre-freezing in equilibrated semen and post-thawing in semen thawed in a water bath at 37oC for 30 seconds. Results showed that PEE2 showed the highest (P < 0.05) positive impact on improving progressive motility, livability, abnormality, and acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) via...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
...e collection, stored and frozen with -20 oC and growth factor concentrations were measured using the ELISA method. The results showed that there were 228 developing embryos (41%) after 48-hour of culture with 2-, 4-, 8-, and 16-cell division. EGF concentrations in the medium of 2-cell to 16-cell ranged from 0.8539 ng/mL-0.2838 ng/mL. The concentration of FGF2 in follicular fluid is 0.629 ng/mL, while the concentration of FGF2 in maturation media, culture media...
Khanitta Pengmeesri1,2* Thassawan Somchan1 , Doungnapa Promket1,3
Nazia Suleman* and Asia Riaz
...her treatments including frozen aphids, frozen eggs of Angoumois grain moth and soaked raisins. Among all treatments, longevity was minimum on frozen aphids (Mean= 66.4 days) for seven spotted beetles. Similarly for zigzag beetle, Menochilus sexmaculatus, the number of days the beetles survived was significantly higher on live aphids (Mean= 106.54 days) and was minimum on soaked raisins (M...
Budi Utomo1*, Amaq Fadholly2,3, Endang Tri Margawati2, Alek Ibrahim2, Ristaqul Husna Belgania1, Muhammad Fajar Amrullah1, Rimayanti1 and Hermin Ratnani1
...d for this study were 10 frozen straw of Limousin cattle and 10 samples of Madrasin cattle. The method used in this research was a descriptive analysis by duplex polymerase chain reaction and analyzing the results of the SRY gene sequencing of limousine cattle and Madrasin cattle. In the present study, we showed that the SRY gene of Madrasin cattle and Limousine cattle could be amplified with SRY F and SRY R primers with a PCR product length of 318 bp. Based o...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
...different times and then frozen. The frozen semen was then stored in a liquid nitrogen container at a temperature of -196°C.After 24 hours, the semen was thawing at a different temperature. The evaluation of semen quality included motility, viability, plasma membrane integrity, and acrosome integrity. All data obtained were analyzed with two way-analysis of variance. The result showed that equilibration time treatment an...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...nal Standard 4869-1:2021 frozen bovine semen.
Keywords | Cryopreservation, Myrmecodia extract, Tris egg yolk, PO bull, Tannin, Protection
...
Nawab Ali1, Akeel Ahmed Memon1, Asmatullah kaka1, Amjad Hussain Mirani2, Muhammad Ibrahim Panhwar3*, Nisar Ahmed Solangi1, Kashif Ali Malik1, Fazul U Rahman1, Moin Akhtar Vistro1
...ed in-vitro (chilled and frozen-thaw sperm traits) and in-vivo conception rate of Kamohri buck semen. ...
Atikah Nur Baity1, Noni Ashri Maghfiroh1, Syalsa Bella Fitriana1, Kurniawan Dwi Prihantoko2, Dyah Maharani2, Diah Tri Widayati2*
...e best quality of thawed frozen semen is obtained at 3 months of storage.
Keywords | Bali bull, DNA fragmentation, Liquid nitrogen, Spermatozoa, Storage periods
... 
