Muhammad Imran1*, Muhammad Mobashar2, Muhammad Irfan3, Shazia Hanif4, Sumaira Hanif5 and Muhammad Abubakar6
...) in lactating Nili-Ravi buffaloes. On the basis of nearly similar lactation stage and body weight, sixteen individually confined buffaloes were randomly blocked and allotted to four different dietary treatments. MOLM in proportion to CSC was supplemented along with maize silage as basal diet. Four dietary treatments having MOLM were 0.0, 0.71, 1.42 and 2.16 Kg DM/day used as replacement for CSC containing 2.16, 1.42, 0.71 a...
Arif Khan, Abdur Rahman, Shoail Akhtar, Siraj Uddin, Zahid Ullah and Nazir Ahmad
...ustify;"> Pakistani buffaloes beef were analyzed for various quality characteristics in relation to breed and age. A total of one hundred and eight beef samples from Longissimus dorsi muscle were collected from three age groups (i.e. group 1= 12-18 monts, 2 =18.1-36.0 months and 3= > 36 months) of Kundi and Nili Ravi breeds of buffalo. Samples were obtained from freshly preselected slaughtered buffaloes at local muni...
Neelum Andaleeb and Munir Khan
...respectively, which show buffaloes were more productive and profitable than cows. Results from Cobb-Douglas production function analysis show that dry fodder, green fodder and daily labor hours have positive significant effect on milk production, while herd size has negative significant effect. Furthermore, the addition of estimated coefficients is less than 1, indicating a decreasing return to scale in milk production. These results suggest that small sized d...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
... biochemical response in buffaloes to Gigantocotyle explanatum infection. Of the 558 slaughtered buffaloes from central Punjab, Pakistan, 18.99% were infected, and morphometric analyses were made of 115 mature amphistomes and gravid eggs with traditional microscopic methods on the basis of standardized measurements. Amphistomes were flattened and stained in Borax carmine, some were sectioned in the median sagittal pla...
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...bortus in cattle and buffaloes in the districts of Islamabad and Rawalpindi, Pakistan. A total of 120 serum samples were randomly collected from buffaloes and cattle (60 per species) at Civil Veterinary Hospitals, animal markets and peri-urban livestock holdings in Rawalpindi and Islamabad. Serum samples were initially screened by the Rose Bengal Plate Test (RBPT). RBPT positive samples were subjected to a B. abortus<...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...gestibility in Nili Ravi buffaloes. Four lactating Nili-Ravi buffaloes of 3rd and 4th parity and milk production of 8±2.3 kg/day were selected and offered a basal diet based on sorghum forage and concentrate ration supplemented with fibrolytic enzyme (Fibrozyme™, Alltech Inc. Company, USA) at four levels (T1 = 0, T2 = 10, T3 = 15 and T4 = 20 g/animal/day) in a 4 × 4 Latin Square chang...
Shahid Hussain Farooqi1,*, Muhammad Ijaz1, Muhammad Hassan Saleem1, Muhammad Imran Rashid2, Muhammad Oneeb2, Amjad Khan3, Amjad Islam Aqib1 and Shakeel Mahmood4
...nd one hundred and fifty buffaloes. Descriptive statistics with Pearson’s Chi-square test and regression model were applied to analyze the data. The results of study found Rhepicephalus the most prevalent genus followed by Heamaphysalis, Hyalomma, Dermacenter and Amblyomma with the prevalence of 78.50%, 10.33%, 10.08%, 0.67% and 0.42%, respectively. On species basis, R. (Boophilus) annulatus, R. (Boophilus) microplus, <...
Kashif Prince1,*, M. Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Jawaria Ali Khan1, Nisar Ahmad3,Rais Ahmed2, Aamerzish Mushtaq4, Sajid Umar4 and Yung-Fu Chang5
...sk factors in cattle and buffaloes of district Kasur, Punjab. Selenium status was evaluated by Atomic Absorptions Spectrophotometery (AAS) with respect to sex of animals, geographical area, age of animals, herd size, stage of animals, production level and concentrate feeding. Selenium deficiency was also evaluated as a risk factor of mastitis and repeat breeding. About 48.43% cattle and 72.39% buffaloes were found positive i...
Abdul Hassan*, Muhammad Ishaq*, Nisar Ali Shah* and Arshad Farooq*
MILK PRODUCTION POTENTIAL IN KHYBER PAKHTUNKHWA
...uction levels. Except in buffaloes and goats where the farm level production of milk is higher than the World and sheep where the farm level production of milk of Khyber Pakhtunkhwa is higher than Pakistan and World. Overall the milk production of Khyber Pakhtunkhwa can be increased upto 5.82mt by proper management and genetic improvement. More research is needed at farm level in the context of breed adaptability and management in respect of cost of livestock ...
Arshed Bashir*, Fatima Ahmad**, Irfan Mehmood*, Muhammad Qasim*, Mazher Abbas* and Sonila Hassan*
ECONOMICS OF RED MEAT PRODUCTION IN PUNJAB
... farms were dominated by buffaloes. The mean age, sale price and live weight of sold buffaloes was 2.8 years, Rs. 64963.8 and 157 kg, respectively whereas for cows the mean age was 3 years with average sale price of Rs. 65722.1 per cow and live weight of 210 kg. The cost of production of meat animals (buffaloes and cows) was higher in commercial farms (Rs. 67820.2 and Rs. 62773.5, respecti...
Khalid Mahmood Aujla* and Abid Hussain*
ECONOMICS OF MILK PRODUCTION OF MAJOR DAIRY BUFFALO BREEDS BY AGRO-ECOLOGICAL ZONES IN PAKISTAN
...tion. Milk production of buffaloes of Nili-Ravi and Kundhi breeds were 2889 and 2375 liter per annum, respectively. Total costs of milk production of Nili-Ravi and Kundhi buffalo breeds were Rs.96155 and Rs.90604 per annum, respectively. Net income per liter from milk of NiliRavi and Kundhi breeds was Rs.12 and Rs.11, and benefit-cost ratios were 1.4 and 1.3, respectively. Hence, Nili-Ravi buffalo breed is more productive and yields better returns over Kundhi ...
Ghazala Nawaz*, Muhammad Nawaz Malik**, Muhammad Hassan
Mushtaq***, Fraz Munir Ahmad****, Ali Abdullah Shah*****, Farooq
Iqbal******, Shinawar Waseem Ali*******, Zahida Fatima******** and
Amjad Khan*********
SURVEILLANCE OF BRUCELLOSIS IN LIVESTOCK IN RURAL COMMUNITIES OF PUNJAB, PAKISTAN
...ence per 1000 animals in buffaloes was (7.86%), significantly (P<0.01) higher than small ruminants (5.84%) and cattle (2.05%). Being a subtropical region, geographically significant (P<0.01) variation was observed in burden and pattern of brucellosis i.e., the highest prevalence was in Central (14.81%) followed by South (0.5%) and northern (0.4%) Punjab. Considering the high mean prevalence and its zoonotic importance it can be devastating if not control...
Abid Hussain1,*, Mansur-ud-Din Ahmad2, Muhammad Hussan Mushtaq2, Mamoona Chaudhry2, Muhammad Sarwar Khan2, Michael Reichel3, Tanveer Hussain4, Amjad Khan2, MuhammadNisar2 and Imtiaz Ahmad Khan5
...inical mastitis in dairy buffaloes. The present study was conducted in two districts Lahore and Bhimber Azad Kashmir Pakistan. Total sample size for this survey was consisted of 1,036 lactating buffaloes. The screening of lactating buffaloes was performed with the California Mastitis Test. The overall prevalence of mastitis was recorded at 49%. Among mastitis cases the prevalence of clinic...
Hasina Baloch1, Rahmatullah Rind2,*, Muhammad Rafique Rind3, Viram Kumar4, Nazia Baloch5 and Rajesh K. Oad6
... subclinical mastitis in buffaloes. Significantly higher prevalence (49. 07%) of clinical mastitis was observed in buffaloes with average age of 6 to 9 years whereas those of > 9 years of age showed higher (33.33%) but non-significant prevalence of subclinical mastitis. However, significant impact of the number of parity on the prevalence of clinical mastitis was observed in animals. Higher prevalence (48.20%) o...
Selçuk Kaplan
...tions in Anatolian water buffaloes. Sequence analysis were revealed seven polymorphic site (G1072A, T1081C, T1131G, T1143C, T1145G, T1151G and C1221T) and one monomorphic site (C1071T) in Anatolian water buffaloes. To the best of my knowledge, this is the first study that fully describes seven polymorphic sites of bubaline leptin gene in Anatolian water buffaloes.
...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
...mined by inseminating 20 buffaloes (10 control and 10 best treatment group). After initial examination semen sample having motility ≥ 70% and morphology live dead ratio ≥ 80 % were diluted in Tris based extender consist of 0,2,4 and 6mM concentration of selenium. Four hours after dilution chilled semen was examined for post chilled semen quality, motility% (74.75±1.9), morphology% (86.62±1.2), membrane integrity% (87.87±0.6), live de...
Abdul Wahaab1, Muhammad Ijaz1,*, Syed Saleem Ahmad1, Umair Iqbal2, Abdul Tawaab1 and Iahtasham Khan3
...ated bovines (cattle and buffaloes) and their effect on various blood parameters (WBC, RBC, Hb, HCT and MCHC). For this purpose, 40 infected animals were selected. After dividing them randomly in four groups of 10 animals each, drug was administered to first three groups whereas last one was kept under positive control for comparison. Study revealed that Triclabendazole was most effective drug of all three in both cattle and buffaloes<...
Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1
... collected from cows and buffaloes kept at different localities in district Kasur, Punjab, Pakistan. The possible risk factors inquired from farmers were animal type, breeds, urbanicity, age (years), teat washing, bedding area, lactating stage and previous exposure of mastitis. Initially surf field mastitis test (SFMT) was applied for screening subclinical mastitis followed by bacteriological techniques on positive milk samples for confirmatory isolation of
Abdul Kabir1, Laiba Uroog2*, Naushad Ahmad3, Fawad Ahmad3, Muhammad Saqib3, Noor Badshah4 and Taj Ali Khan3
... mastitis in a cohort of buffaloes at Khyber Pakhtunkhwa. 120 quarter samples were collected from suspected buffaloes in selected areas of Peshawar, Charsadda, Mohmand Agency, and Dara Adam Khel. Initially, California Mastitis Test was performed for screening of positive samples. Afterward, the bacterial profile was confirmed through biochemical testing. The quarter wise prevalence of subclinical mastitis was 25%. Within thi...
Asghar Khan1,*, Aneela Zameer Durrani1, Arfan Yousaf2, Jawaria Ali Khan1, Mamoona Chaudhry1, Mumtaz Ali Khan3, Habibunnabi4 and Amjad Khan5
...al mastitis in lactating buffaloes in Pothohar region of Pakistan. A questionnaire based cross-sectional based survey was conducted in 30 commercial and subsistent farms in Pothohar region of Rawalpindi district, Punjab. Where randomly 196 lactating buffaloes were sampled. Data on several health, management and bio-security variables was collected in face to face interviews from farmers, managers and owners. Milk samples col...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
...e ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium alone, Group II: SOF + co-culture, Group III: Conditioned SOF, Gr...
Rais Ahmed1,2,*, Muhammad Khalid Mansoor1,2, Iftikhar Hussain1, Muhammad Saqib3, Muhammad Hammad Hussain4, Amjad Islam Aqib5, Haleema Sadia6, Javed Muhammad7, Asma Irshad8, Kashif Prince5, Muhammad Zain Saleem9 and Abdul Whab Manzoor10
...ic disease of cattle and buffaloes, causing progressive weight loss, persistent diarrhea and finally death. Due to zoonotic nature of the disease, workers in slaughterhouses are at a high risk of infection. In this study, the status of paratuberculosis was assessed in a slaughterhouse located in district Faisalabad, Pakistan. A total of 455 blood samples were collected from slaughtered cattle and buffaloes at random and then...
Muhammad Abdul Rahman1*, Abdul Saboor1, Gulnaz Hameed1, Ghulam Bilal2 and Farooq Tanwir3
...seases, sensitiveness of buffaloes to temperature, slow and reduced weight gain and effect on birth and death rate are weighted high as threat to farm animal health while India-Pakistan ties on water, water scarcity, high water requirement for buffaloes and lack of innovation in processing of milk and milk products are other major threats to production from farm animals. The findings of this study can serve as basis of provi...
Savas Atasever*, Huseyin Erdem and Ibrahim Cihangir Okuyucu
...men fill scores (RFS) in buffaloes. In total, 66 Anatolian buffaloes reared in four farms of the Middle Black Sea region of Turkey were scored subjectively by two parameters. BCS and RFS data were obtained using a 1 to 5 scale and a 1 to 4 scale, respectively, where lower points indicate thin body condition or relatively empty rumen. Animals were examined in two subgroups by parity (1= lactation number 1 and 2; and 2= l...
Saeed Murtaza1, Abdul Sattar1*, Nasim Ahmad1, Muhammad Ijaz2, Maqsood Akhtar3 and Muhammad Shahzad4
Ertuğrul Kul*
...) records from Anatolian buffaloes calved during 2014-2017 raised under different farm conditions in Amasya Province, Turkey. Five different lactation curve models such as Wood (WOD), Cobby and Le Du (CLD), Exponential (EXP), Parabolic Exponential (PEXP) and Quadratic (QUA) models were used. The adjusted multiple coefficient of determination (R2adj) and the residual standard deviation (RSD) were evaluated as parameters to detect th...
Hifzulrahman1, Muhammad Abdullah1*, Muhammad Uzair Akhtar2, Jalees Ahmed Bhatti3, Muhammad Saadullah1 and Muhammad Naveed-ul-Haque2
...arly-lactating Nili Ravi buffaloes. Sixteen early-lactating Nili Ravi buffaloes received 2 treatments in a completely randomized design with a period length of 120-d. The treatments were 1) Ctrl = basal diet without supplementation of fat, 2) Ca-FA = basal diet with calcium salts of palm fatty acids supplementation. The Ca-FA treatment increased the 3.5% fat-corrected milk yield by 11.0% compared with the Ctrl, whereas, the ...
Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan2
...tating indigenous cattle/buffaloes raised on different commercial dairy farms and exposing the hazards linked with milk-born tuberculosis infection in the area where unpasteurized milk is normally used in the central zone of Khyber Pakhtunkhwa (KP), Pakistan. A total of 1225 cattle and 1175 buffaloes of more than one year were studied for the prevalence of bovine tuberculosis. A total of 1608 milk samples (793 from cattle an...
Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2
A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
...the households possessed buffaloes. Average number of buffaloes was estimated at 4.28 while 376 (63%) of the household reported cattle with average number of 4.38 heads per household. Small numbers of buffalo and cattle bulls were recorded. Average number of 5.55 goats per households was reported by 35% of all the households. On an overall basis, 2,365 liters of buffalo milk was computed from 600 households, while 1,033 lite...
Rabea Ejaz1*, Saima Qadeer2,Muhammad Sajjad Ansari3, Bushra Allah Rakha4, Shazia Shamas5, Iram Maqsood1, Asma Ul Husna6, Asima Azam1 and Shamim Akhter6
...ignificantly improved in buffaloes inseminated with semen containing 20.0 ng/mL of arachidic acid (58.64%) compared to control (46.06%). In conclusion, addition of arachidic acid (20.0 ng/mL) in extender significantly enhanced quality and in vivo fertility of post thaw buffalo semen.
...
Mudussar Nawaz1, Iahtasham Khan2, Muhammad Shakeell*, Arfan Yousaf1, Zahid Naseer1, Munibullah1, Ali Zohaib3, Riaz Hussain4 and Qudrat Ullah5
...alence of brucellosis in buffaloes and goats of Islamabad Capital Territory, Pakistan. A total of 341 milk samples from buffaloes (n=180) and goats (n=161) were screened by (Milk Ring Test) MRT and milk i-ELISA for anti-Brucella antibodies. Higher prevalence was found in buffaloes (5.6% and 16.1%) than in goat (4.97% and 1.9%) both through MRT and i-ELISA respectively. Commercial pr...
Said Sajjad Ali Shah1*, Muhammad Ilyas Khan1, Aziz Ullah1, Hayat Ullah2 and Faisal Ahmad2
Coprological Examination of Small and Large Ruminants in Central Zone of Khyber Pakhtunkhwa
...asciola (6.1%), while in buffaloes fasciola was recorded higher followed by amoeba (19%) and Trichostongylus (5.35%). Haemonchus contortus was the most prevalent intestinal parasite in the study area followed by Trichostrongylus in small ruminants, whereas mixed infection in goats was recorded as 21%. Different predisposing factors studied in the current study were season, location, age and sex. Prevalence was recorded higher in hot and humid months of the yea...
Eman Khalifa1*, Riad Khalil2 and Haitham Elaadli3
...bacterium bovis from buffaloes’ mastitic milk by isolation on Lowenstein-Jensen medium (LJ medium), Ziehl-Neelsen staining; identification by different biochemical tests and polymerase chain reaction (PCR) at 500bp diagnostic for M. bovis, as well as (2) to determine the therapeutic efficacy of the various antimicrobials (9 types) on the isolated M. bovis by using different antimicrobial sensitivity plate method. A total number of 100 s...
Ali Zaman1, Nabila Roohi1 and Muhammad Irfan2*
...iochemistry in Nili Ravi buffaloes under subtropics is lacking. This study was designed to fulfill this gap by analyzing the effects of exposure to the bacterial culture of Pasturella multocida (P. multocida) type B:2 and its immunogens, i.e., lipopolysaccharide (LPS) and outer membrane protein (OMP) on Nili Ravi buffaloes. Prepubertal Nili Ravi female buffaloes (N=18) of 10-12 months age ...
Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2
...alves of cattle (32) and buffaloes (8) below three months in some farms in Egypt. The detection was done by ELISA and indirect fluorescent antibody technique (FAT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal diarrhea. Six out of forty tested samples were found positive for rotavirus (15%) infection using ELISA test. Successful isolation of the rotavirus on MDBK cell line from ...
El-Bagoury, G.F., El-Nahas, E.M., El-Habbaa, A.S.
...ally infected cattle and buffaloes. Twenty Buffy coat samples were collected from each clinically infected cattle and buffaloes in Qaluobia province during the year 2014. The virus was isolated on BHK-21 cell line. Infected cells with BEFV showed 80% cytopathic effect (CPE) after 48 h by the 3rd virus passage. The isolates were serologically identified by neutralization and immunoflourescence techniques, and then molecularly...
Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3
...fecting cattle and water buffaloes. The present work aimed to isolate the causative agent and identify it by serological characterize of the viral antigens, then molecular characterization of its genome. Infection by the bovine ephemeral fever virus (BEFV) was noticed in Dakahlyia governorates during the summer season of 2015. Twelve samples of buffy coats were collected from dairy farms suspected from clinical investigations to be infected by bovine ephemeral...
Nasir Ali Tauqir1, Asim Faraz2*, Muhammad Arif1, Abdul Rehman1, Imtiaz Hussain1, Abdul Waheed2, Gabriele Marino3 and Michela Pugliese3
...y to lactating Nili-Ravi buffaloes on their nutrient’s intake and utilization, milk yield and composition, nitrogen balance and hematological parameters. Fifteen lactating Nili-Ravi buffaloes in mid lactation with almost similar milk production were randomly divided into three different groups of five females within each according to completely randomized design. Three iso-caloric and iso-nitrogenous diets with high, m...
Muhammad Anwarullah1, Aneela Zameer Durrani1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Muhammad Usman1*,Muhammad Zahid Iqbal1, Muhammad Husnain1,Ahsan Anjum3, Qurratulain Ashraf4 and Ghulam Mustafa3
...P was 2.20 times more in buffaloes than in cattle; 21.19 times more in females than in males; 1.04 times more in adult animals having age >6 years; and 1.20 times more in dry animals compared to milking animals. Furthermore, body condition score (BCS), type of housing, and condition of drinking water were also found to have significant (p<0.05) effect on the occurrence of MAP in cattle and buffalo. Moreover, hematological profile of MAP-positive animals ...
Bikash Puri1, Anil K. Tiwary2, Bharata Regmi3,4, Dinesh K. Singh1, Doj R. Khanal5 and Manoj K. Shah3*
Sharjeel Saif1, Asghar Ali Kamboh1*, Ghulam Mustafa Solangi2, Rehana Burriro3, Hasina Baloch1, Atta Muhammad Memon2
... from mastitis suspected buffaloes. The samples were collected from those animals which had abnormal milk or physical signs (like swelling, abscess, wound etc.) on udder or teats or have suddenly dropped milk production. All samples were cultured on PPLO (pleuropneumonia like organism) media and were found negative for Mycoplasma spp. The prevalence of mycoplasma mastitis in this population of dairy buffaloes was recorded &l...
Sonali Menamvar1,2,3, Kirubakaran Vinod Kumar1, Veeregowda Muniveerappa Belamaranahally2, Yella Narasimha Reddy3, Rathnamma Doddamane2, Shrikrishna Isloor2, Ramesh Poojari Thimmaiah2, Gurrappanaidu Govindaraj1, Bibek Ranjan Shome1, Vinayagamurthy Balamurugan1*
...seases of the cattle and buffaloes population worldwide and is caused by serovars of pathogenic Leptospira. A pilot study was conducted in some of the dairy farms located in Telangana and Andhra Pradesh, endemic states of India, during 2017 to investigate the seropositivity of bovine leptospirosis in dairy animals and associated risk factors at the farm level. The semi-structured questionnaire was designed as per the literature and used to collect leptospirosi...
Mohamed Abd El-Fattah Abo-Farw1, Maged Ahmed Aboul-Omran1, Abdel-Khalek El-Sayed Abdel-Khalek2*
...ace elements in Egyptian buffaloes. A total of 90 multiparous lactating Egyptian buffaloes (500-600 kg live body weight, 5-7 years old, 3-5 parities, and 10.75 ± 2.66 kg/day milk yield were used in this study. The buffaloes were managed and feed at similar traditional conditions. Mastitis diagnosis was performed 21 days after calving, then animals (n=90) were grouped to normal (n= 7...
Muhammad Zahid Iqbal1, Aneela Zameer Durrani1, Jawaria Ali Khan1, Nisar Ahmad2, Muhammad Usman1,*, Abdul Jabbar1, Saba Usman3, Ahsan Anjum3, Muhammad Husnain1, Nadeem Raza1 and Anwar-ul-Haq4
...2% and 12.5% in cows and buffaloes, respectively. The association of selected clinical biomarkers was also ascertained and all of these were found significantly (p<0.05) associated with the incidence of coxiellosis. The results of animal-related risk factors indicated that with increasing age and parity number, poor BCS, or a history of disease, the incidence of coxiellosis became significantly (p<0.05) higher. Various farm-related risk factors were also...
Wafaa M.A. Ghoneem*, Muhammad M. Shaklouf, Ali M. Ali and Mohammed H. Bakr
...stify;">Thirty lactating buffaloes (650 ±50 kg average body weight, in the first to the fifth seasons of lactation, 127±4 Days in milking and average of 12.32±1.13 kg daily milk yield) were divided into three similar groups to evaluate the effect Lactobacillus acidophilus fermentation product (LAFP) commercially named (Culbac®) on nutrients digestibility, milk yield and composition, blood biochemistry and immune system response of the ...
Sher Bahadar Khan1,*, Mumtaz Ali Khan2, Irshad Ahmad3, Faheem Ahmad Khan4, Hamayun Khan1 and Sher Ali Khan5
...f 1250 milk samples from buffaloes were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes through PCR. Among them, the highest prevalence of Staph. aureus was found in Peshawar-Mardan division (30%), followed by Malakand (28.5%), Bannu-Dera Ismail khan division (25%) and Hazara division (16%). Over all the high resistance was found against Lin (96.25%) followed by AMX (82.5%), TET (63....
Muhammad Zaeem Abbas1, Khurram Ashfaq1, Amjad Islam Aqib2, Mughees Aizaz Alvi1*, Muhammad Haleem Tayyab1, Muzafar Ghafoor1, Muhammad Usman Naseer3, Ali Abbas4 and Ali Hassan1
...ed risk factors in dairy buffaloes. In this regard, milk and urine samples were individually collected from 96 buffaloes and analysed by Ross Modification of Rothera’s test and Abbott Precision Xtra™ meter to assess the ketone bodies levels. We observed a total of eight out of 96 (8.33%) buffaloes positive for ketosis. The peak prevalence (13.3%) of ketosis was detected during ...
Ngoc Tan Nguyen1*, Pham Kim Ngan Nguyen1, Thao Huong Phan1, Tan Loi Le1, Thi Khanh Ly Nguyen1, Tuan Thanh Hoang2, Cong Thieu Pham3, Cong Dinh Nguyen3, Nguyen Khang Duong4
...amples) and two imported buffaloes from Thailand (T: 5 samples) and India (Murrah – M: 5 samples) were extracted for total DNA and amplified about 760 bp mtDNA fragment, with 576 bp in the D-loop region, followed by sequencing to analyze genetic diverse indices and genetic distance to construct a phylogenetic tree. Results showed that the fragment of 760 bp was successfully amplified. Variations of nucleotide sequence in 576 bp fragment length from 31 in...
Tariq Ali1,2*, Kamran1, Abdur Raziq1, Inam Ullah Wazir1, Anwar Ali1, Muhammad Ijaz Ali1, Muhammad Shuaib Khan2, Shakeeb Ullah2 and Sher Hayat Khan3
...ases in dairy cattle and buffaloes. This study describes the current prevalence of mastitis in lactating cattle, and buffaloes in Khyber Pakhtunkhwa during 2019-2020, to identify bacterial pathogens of mastitis, and to determine the antimicrobial resistance. A total of 1013 milk samples belonging to 726 cattle and 287 buffaloes were examined to detect clinical mastitis (CM) and subclinical...
Lobna M.A. Salem, Nashwa O. Khalifa, Marwa O. Abd El-Halim*
...infecting sheep, cattle, buffaloes, goats, and camels in Qualyobia, Egypt. The genetic identity of Fasciola species was examined by the analysis of forward and reverse sequences of the ITS2 of the rDNA gene that amplified in 300bp. Out of 286 slaughtered animals {88 sheep, 26 goats, 68 cattle, 25 buffaloes and 79 camels} in the regions of Qualyobia, 51 (17.8%) had Fasciola spp. morphologically {27/51(52.9%) were identical to...
Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1*
...estrus cycle in Egyptian buffaloes. Hence, the study of molecular control of the CL in Egyptian buffaloes let us to know the mechanisms of this regulation. We aimed to (1) figure out the expression profile of apoptotic genes (TNFα- BAX - CASP3 - FASLG - AGTR2 - and NOS2) mRNAs during early, mid, and late stages of CL and (2) clarify the accompanied changes in progesterone (P4), nitric oxide (NO) concentrations and hist...
Sherif Abdelghany1, Hossam Mahrous Ebeid2*, Ahmed Abdelkader Aboamer2, Mohamed Ali Radwan1, Rania Agamy1
...crossbred cows and local buffaloes in mid-lactation, with an average of 4.4 parties and an average herd size of 1.28 and 1.19 head/farm for Nile Valley and Newly Reclaimed districts, respectively. A structured questionnaire survey was designed, and field interviews were conducted to collect data and to characterize the current situation. Representative feed staff and milk samples were collected and analyzed. Feeding practices efficiency indicators were calcula...
Aqsa Batool1, Masood Rabbani1*, Farhat Nazir Awan2, Sohail Raza1, Sehrish Firyal3 and Shahan Azeem1
... in cattle and 20-75% in buffaloes. Age, breeds of animals and milking status were not found to be potential risk factors for the disease. Logistic regression demonstrated that herd size (>thirty), parity (>2) and abortion history are potential risk factors associated with seroconversion of BoHV-1....
Ahmed Fathy1, Rania A. Hassan2, Essam S. Soliman3*
...ification between Baladi buffaloes and crossbred using clinical epidemiological measures of some productive and reproductive traits such as milk yield (MY/kg), days in milk (DIM/days), age at first calving (AFC/months), calving interval (CI/months), days open (DO/days), dry period (DP/weeks), number of services per conception (SPC rate), and days to first insemination (DFI/days). The raw data on 927 buffaloes (763 crossbred ...
Hussein Jabar Jasim1*, Amer Murhum Al-Amery2
... changes in the infected buffaloes with fasciolosis. Also, the current study recognized the gross and histopathological changes resulting from the invasive Fasciola spp. in the liver and gallbladder. The biochemical results showed statistically significant increases in the serum total bilirubin, creatinine, and active serum enzymes ALT, AST, ALP, and GGT. In contrast, the serum total albumin showed a significant decrease in infected bu...
Hanaa M. Abdelkhalek1, Hanan E. Nagib1, Randa S. Elias1, Saad S. Mansour1, Walid S. Mousa2*
...in most dairy cattle and buffaloes herds. Pseudomonas aeruginosa (P. aeruginosa) is opportunistic pathogens involved in veterinary disorders including clinical mastitis in buffaloes. This study aimed to investigate the antibiogram pattern and synergistic effect of amikacin and norfloxacin against resistant P. aeruginosa isolates from mastitis origin. In addition, detection of some virulence and antibiotics resistance genes. ...
Nasir Ali Tauqir1*, Asim Faraz2, Abdul Waheed2, Ayman Balla Mustafa3, Irfan Shahzad Sheikh4, Michela Pugliese5 and Shahid Nazir6
...n in lactating Nili-Ravi buffaloes. Sixteen early lactating nili-ravi buffaloes were divided into four groups according to Randomized Complete Block Design for this study. Four experimental diets were formulated supplementing 0, 15, 25 and 35g of methionine/ animal/ day. The experimental period was of 56 days out of which 10 days were as adaptation period. Daily feed intake and morning-evening milk yield of each animal was r...
Navjot Hothi
...ainly affects cattle and buffaloes. India has the highest livestock population in the world, and this provides a significant economic contribution to small-scale farmers. The summer and monsoon months of 2022 witnessed a sudden spurge across 14 states and 1 union territory in India, with a spread spanning 251 districts and 43,759 epicenters. Around 2.4 million cattle were affected in India, and 0.11 million cattle succumbed to the disease. The 10 most affected...
Fadzlin Afiqah A. Samad2, Amirul Faiz Mohd Azmi1, Muhamad Affan Ab Azid1, Hafizin Mu’izz Zalazilah1, Syakirah Zulkifli1, Izreen Edriana Mohd Jasmi1, Muhammad Baqir Irfani Rahimin Affandi1, Mohd Zamri Saad1, Md Zuki Abu Bakar1, Agung Irawan3,7, Adib Norma Respati4, Anuraga Jayanegara5,7, Sadarman Sadarman6,7, Hasliza Abu Hassim1,2,7*.
...ns from multi-species of buffaloes were analyzed according to a linear mixed model methodology with explanatory variables declared as fixed effects and individual study as random effects. The results showed a negative curvilinear pattern of milk yield across buffaloes’ breeds in response to the increasing FC ratio (P<0.05; R2 = 0.828) and strong linear increased in response to the increasing DMI (P<0.01; R2 = 0.8...
Amirul Faiz Mohd Azmi1,4, Hafandi Ahmad1, Norhariani Mohd Nor1, Goh Yong Meng1, Mohd Zamri Saad2, Md Zuki Abu Bakar1, Norafizah Abdul Rahman5,6, Agung Irawan7,9, Anuraga Jayanegara8,9, Hasliza Abu Hassim1,3,9*
... enhancing the growth of buffaloes, the effect on a different breeds of buffaloes, the potential to reduce methane production, and the changes in microbial populations remained unclear following different ratios of forage: supplementation feeding regime. This study described the effects of supplementing Brachiaria decumbens grass (G) received either concentrate (C) or mixed with bypass fat (B) supplement on the in-vitro rume...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...served semen of Egyptian buffaloes.
Keywords | Egyptian buffalo, Propolis extract, Cryopreservation, Semen variables, Lipid peroxidation
...
Salam N. Aritonang*, Elly Roza, Rizqan, Winda Habsani
...between Swamp and Murrah buffaloes in Humbang Hasundutan Regency, North Sumatra, Indonesia by measurement of body size and body weight. We used 80 buffalo crosses at ≤ 6, 7−12, 13−18, and 19−24 months of age. The research methods used census and survey methods with purposive sampling techniques for determining the research location. We observed variables such as body weight, chest circumference, body length, and shoulder height. Data analy...
Jyotsana Shakkarpude1*, Aditya Mishra2, Deepika D. Caesar2, R.K. Sharma3, Anand Kumar Jain2, Sanju Mandal2, Rajesh Kumar Vandre4, Danveer S. Yadav5, Bhavna Ahirwar6 and C.P. Solanki6
...n appetite of postpartum buffaloes which directly leads negative energy balance (NEBAL) and increases oxidative stress in animals. Betaine is a growth promoting nutritional additive used in livestock. Betaine contains three methyl groups and thus acts as a methyl donor for metabolic reactions. Betaine acts as molecular chaperone, as it repairs heat shock proteins. The present study was aimed to investigate the effect of dietary betaine on heat and metabolic st...
Ghani Khan1, Saeed Ahmed Soomro1, Abdul Kabir2, Abdullah Iqbal3*,Naik Muhammad Marri 4, Syed Ahmad khan5, Muhammad Roidar Khan6 , Anees ur Rehman1, Muhammad Zakir Khan7
...ed from thirty lactating buffaloes, divided into a control group and an oxytocin-treated group, and further subdivided by lactation. The samples were analyzed for pH, specific gravity, ash, protein, total solids, fats, lactose, and moisture content. The results showed that oxytocin treatment significantly decreased pH levels in milk from the first lactation, while specific gravity did not differ significantly between the control and oxytocin-treated groups. To...
Abdul Kabir1*, Anees Ur Rahman2, Inzamam Ali Shah2, Ibad ur Rahman2
...al zoonosis that affects buffaloes, cows, and humans. It is caused by buffalopox virus (BPXV), a member of the genus Orthopoxvirus and a close variant of vaccinia virus (VACV). Buffalopox was first reported in India in 1934 and has since become an emerging and re-emerging disease in the Indian subcontinent and other countries. The disease is characterized by pock lesions on the skin and mucous membranes of the affected animals and humans, with complications su...
Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3
...e response of cattle and buffaloes against P. multocida B:2 in developing countries like Pakistan....
Hassanein, Suzan A.; Abd El-Wahab, Wafaa; Eweis, Moustafa and Mahmoud, Mervat M.
Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.
...ing 2009 from cattle and buffaloes. The higher percentage of positive was detected in Sharkia (31.7%) while Kafr Elsheikh was (27.3%). The positive result of detection of antibodies against non structured proteins of FMDV indicates that these samples come from natural infected animals. The further work is required to evaluate the performance of this method in different animal species and different field situations. The Prio check ELISA was also used for compar...
Abeer, E.Mansour and Hegazi, A.Z.
...ed in vaccinated cattle, buffaloes, sheep, goats and camels. Five animals of each species were vaccinated using a dose of I ml for sheep and goats and 2 ml for cattle, buffaloes and camels inoculated subcutaneously. It was found that both of the immune parameters were generally increased gradually to reach the highest value of cellular immunity by the 2e day, while that of neutralizing antibodies was reached by the wee...
Nahed A. Mohamed l, H. A. Hussein2, Fathia M. Mohamed1 and M. A. Shalaby2
...s from apparently health buffaloes. One hundred and thirty-two samples including buffy coat and organs were collected from apparently health Screening buffaloes in Cairo, Mansoura and Suez governorates in Egypt during the year 1999. Screening of samples by GAPT using standard known anti-BVDV antisera revealed that three positive samples for the presence of BVDV antigen. In a trial for the isolation of the BVDV, 69 buffy coat...
H. A. Hussein1, N.A. Mohamed2, F.M. Mohamed2 and M.A. Shalaby1
...d from apparently health buffaloes in cell culture was studied. The isolated viruses were classified into 3 groups based on the onset and severity of cytopathic effect (CPE). The cytopathogenicity of the isolates were different when these viruses were inoculated on BVDV-free and-infected cell lines. To study the discrepancies on the growth behavior of the local isolates, the viruses subjected to further passages on MDBK cells infected with non-cytopathic (NCP)...
Elly Roza*, Salam N. Aritonang, Yulia Yellita, Hilda Susanty, Rizqan
...using four female Murrah buffaloes as research samples with the following feeding: P1 = basal feed (10% of body weight); P2= 30% sweet potato leaves + 30% cassava leaves + 40% of P1 + urea saka block (USB); P3 = 40% sweet potato leaves + 40% cassava leaves + 20% P1 +USB; P4 = 50% sweet potato leaves + 50% cassava leaves + USB. The parameters measured in this study were the hematology (erythrocytes, leukocytes, hemoglobin, and hematocrit) of Murrah buffalo. The...
Mahmoud Hamouda1*, Ahmed Al-Jazzar1, Fahad Al-Hizab1, Ahmed Azab2, Mohamed Al-Hammadi3
...ductive tracts of female buffaloes have a previous history of infertility was collected from different farms in Egypt through the period of two years. Uteri have been visually and manually inspected as well as examined bacteriologically and histologically. Several uterine lesions either external or internal were noted in 78/340 animals (22.94%) as follows: internal lesions as endometritis in 33 animals (9.71%), metritis in 11 animals (3.24%), endometrial hyper...
Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2
...um) of two riverine type buffaloes using PCR-denaturing gradient gel electrophoresis (DGGE) method to profile the bacterial community and PCR amplification using species-specific primer sets for 16S rDNA fragments to detect major fibrolytic and non-fibrolytic bacteria. Results from the PCR-DGGE analysis showed differences in bacterial diversity, density, and banding patterns along the gut of buffaloes. Higher bacterial diver...
KAFEEL AHMAD1, NIMRA ARSHAD1, ZAFAR IQBAL KHAN1, KINZA WAJID1, HUMAYUN BASHIR1, IJAZ RASOOL NOORKA2, MAHPARA SHEHZADI3, HAFSA MEMONA4, MADIHA SANA4, KHALID NAWAZ5, MUDASRA MUNIR1, IFRA SALEEM MALIK1, TASAWAR ABBAS6, ABRAR HUSSAIN7, MUHAMMAD SHER8, MUHAMMAD FAHAD ULLAH6, MUHAMMAD AKRAM QAZI9, MUHAMMAD NADEEM10, MUHAMMAD RIZWAN QURESHI10
Wesam Jasim Hansh
...Fascioliosis in cows and buffaloes in Thi-Qar province, Iraq based on morphological measurements and sequences analysis of 28S rRNA gene. Fasciola flukes were isolated from the livers of cows and buffaloes slaughtered in the municipality of AL-Nassiriyah abattoir during July to November 2022. Sixty Fasciola flukes including (30 from cows and 30 from buffaloes) were used in morphological st...
MUSHTAQ HUSSAIN LASHARI1*, UMER FAROOQ2, AMMARA ARSHAD1, MUHAMMAD NAEEM TAHIR2, MUSADIQ IDRIS2, ANAM KHURSHID3 & ZIA UR REHMAN2
... adult cattle (n=26) and buffaloes (n=26) grouped as males and females (n=13 each), were collected from the jugular vein through appropriate measures. Thin blood smears were stained using Field stain „A‟ and „B‟. The PMNs were counted and observed for Barr Bodies attached to them. Only the neutrophils with terminable lobes were examined and counted. The mean value ±SE of the Barr Bodies was calculated and the difference between males and...
Gokarna Gautam1*, Sajan Rokaya1, Bhuminand Devkota1, Saroj Sapkota2
...>Reproductive pattern in buffaloes (Bubalus bubalis) is affected by photoperiodicity, heat stress and availability of feed supply. The objectives of this study were to understand the seasonality pattern of breeding and calving in buffaloes in subtropical regions of southern Nepal and to characterize the ovarian cyclicity status during the months of long day length. Data regarding seasonality pattern of breeding and calving o...
Anisa Mushtaq1, Murtaz ul Hasan1*, Asim Shamim2, Muhammad Ali Abdullah Shah1, Muhmamad Arif Zafar3, Abdul Asim Farooq4, Aayesha Riaz1, Muhammad Kamran1 and Saif ur Rehman1
... from 384 cattle and 384 buffaloes screened revealed three tick genera and six tick species: Rhipicephalus microplus, Rhipicephalus decoloratus, Rhipicephalus annulatus, Hyalomma anatolicum anatolicum, Hyalomma anatolicum excavatum and Haemaphysalis punctata. Of those four species were confirmed on morphological basis, two ticks species Rhipicephalus microplus and Hyalomma anatolicum anatolicum whose morphological feature were overlapping with other identified...
Gokarna Gautam1*, Hari Adhikari1, Bhuminand Devkota1, Subir Singh2
...treatment of anestrus in buffaloes during low breeding season (April to June). Buffaloes in Group T1 (Ovsynch, n=25) received GnRH on a random day (d0), PGF2α on d7, GnRH on d9, followed by fixed timed artificial insemination (FTAI) at 16-20 hours after second GnRH. Buffaloes in Group T2 (CIDR-synch, n=30) were treated as in group T1 except that a CIDR containing 1.9 gm progest...
