Issue · 2026
Pakistan J. Zool., Vol. 58, Iss. 1, pp. 01-500
62 articles · pp. 01-500 · All issues →
Research ArticleEffect of Subclinical Lead Toxicity on Hematological and Serum Biochemical Profiles of Damani Sheep
Muhammad Umer Farooq, Muhammad Sajid, Aziz Ur Rehman, Amar Nasir, Habib Ullah, Muhammad Mubeen et al.
Heavy metals are known to have harmful effects on both human and animal health. Among these toxic metals, lead is particularly damaging, affecting the kidneys, brain, liver, and genetic integrity of both humans and animals. This study aimed to assess the level…
24 Nov 2025DOI 10.17582/journal.pjz/20240912101714
Research ArticleComparative Evaluation of Quail Egg Yolk-Based Extender for Semen Cryopreservation in Baggara Bulls
Mohamed A. El-Khalifa, Bashir Salim, Safaa Abdelrahim, Zeinab A.E. Osman, Ahmed M. Khalid, Saeed Alasmari et al.
This study evaluated the efficacy of a quail egg yolk-based extender for the cryopreservation of Baggara bull semen, compared to hen egg yolk and a commercial egg yolk-free diluent (IMV). Baggara cattle, a hardy breed native to Sudan and known for their adapta…
14 Dec 2025DOI 10.17582/journal.pjz/20241222085027
Research ArticleEvaluation of Biofloc Meal Supplementation on Growth and Physiological Responses of Pacific White Leg Shrimp, Penaeus vannamei in Outdoor Culture System
M. Nethaji, B. Ahilan, A. Kathirvel Pandiyan, N. Felix, A. Uma, P. Ruby
The evaluation of alternative protein source in shrimp feed is a priority in the aquaculture industry. Biofloc meal is a substantial protein source of shrimp feed, which has been examined by various studies under experimental conditions. The present study exam…
21 Dec 2025DOI 10.17582/journal.pjz/20241005150734
Research ArticleEvaluation of Phage-Antibiotic Synergism Against Salmonella enteritidis Isolated from Broiler
Kaleem Ullah, Rabia Kanwar, Sania Mubeen, Rimsha Kanwal, Saim Ahmad, Gulshan Ara et al.
Salmonella enteritidis is a facultative intracellular zoonotic pathogen and has one health importance. Antimicrobial-resistant strains can be found in poultry, especially broiler, and can act as a carrier to spread these diseases to humans. Lytic bacteriophage…
27 Dec 2025DOI 10.17582/journal.pjz/20240727150818
Research ArticleSeasonal Fluctuations in the Dietary Habits of Indian Crested Porcupine (Hystrix indica Kerr) in Pothwar Plateau, Pakistan
Ayesha Sheraz, Tariq Mahmood, Muhammad Mushtaq, Ahmed Junaid, Shaista Andleeb, Unza Waqar et al.
Understanding ecological traits, such as diet analysis of wildlife species, is crucial, especially when the species is categorized as a pest. One such important pest in the agroecosystem of Pakistan is the Indian crested porcupine (Hystrix indica Kerr), being…
27 Dec 2025DOI 10.17582/journal.pjz/20240501125036
Research ArticleGenetic Variants in Mucopolysaccharidosis Type I Patients from Pakistan: Identification of a Novel 14-Nucleotide Deletion and A Highly Prevalent Missense Variant in a Cohort of 10 Families
Rutaba Gul, Sabika Firasat, Mulazim Hussain, Fatima Akbar, Kiran Fatima, Muhammad Farooq Sabar et al.
Autosomal recessive mucopolysaccharidosis-I (MPS-I), an inborn error of metabolism, in which heparan sulfate and dermatan sulfate accumulate in the cells due to deficiency of enzyme alpha-iduronidase (IDUA) is more common in consanguineous populations. Previou…
29 Dec 2025DOI 10.17582/journal.pjz/20220416090446
Research ArticleIdentification of New Isolates of Classical Swine Fever Virus Based on Untranslated Regions with Traditional and Machine Learning Classification
Madiha Fatima, Iqbal Nisa, Yongjin Wu, Iqbal Ahmad, Batool Fatima, Faiz Ur Rehman et al.
Classical swine fever is a deadly and economically significant pig disease caused by the classical swine fever virus (CSFV). This disease is one of the most contagious viral infections affecting pig herds. Since late 2014 China has had a rising number of C-str…
1 Jan 2026DOI 10.17582/journal.pjz/20240625204553
Research ArticleComparative Analysis of Various Extenders for Chicken Semen Preservation and its Effects on Hatchability and Post-Hatch Performance of Fayoumi Chicken
Zafar Ali Khoso, Nasir Rajput, Imdad Hussain Leghari, Asmatullah Kaka
This study investigated the effects of different extenders on chicken semen preservation and their impact on hatchability and post-hatch performance in Fayoumi chickens. Twenty-five males and two hundred fifty females were divided into five treatment groups: T…
29 Dec 2025DOI 10.17582/journal.pjz/20240612043039- Research Article
Effect of Selenium on Morphology, Mineral Retention of Tibia Bone and Muscle Health of Broiler under Dexamethasone-Induced Stress
Tamreez Khan, Asad Ullah, Imad Khan, Tahira Tayyeb, Shafqat Ullah, Rafiq Ullah et al.
In current research study, the antioxidant activity of inorganic selenium (Se) for broilers under induced stress was determined to improve the muscle and tibia bone health as well as tissue mineral retention. The poultry industry is playing a vital role in ful…
1 Jan 2026DOI 10.17582/journal.pjz/20231024060504 
Research ArticleProtective Effect of Selenium and Curcumin against Pasteurella multocida Challenged Hematological and Biochemical Alterations Leading to Hepato-Renal Injury in Rabbit
Fayaz Ali Ujjan, Suhail Ahmed, Saba Parveen Samo, Fahmida Parveen, Zaheer Ahmed Nizamani, Moolchand Malhi et al.
The current study investigated the role of selenium (Se) and curcumin (Cur) in alleviating the severity of Pasteurella multocida (P. multocida) infection associated with hematological and biochemical variations. Twenty rabbits were randomly assigned into four…
1 Jan 2026DOI 10.17582/journal.pjz/20241029124603- Research Article
Molecular Characterization and Genetic Classification of Pakistani Freshwater Fishes through DNA Barcoding
Lala Rukh, Muhammad Tayyab, Rana Manzoor Ahmad, Yusra Mateen, Madeeha Awan, Muhammad Ashraf et al.
Pakistan is endowed with an extensive network of fisheries resources. Approximately 193 fish species are present in fresh waters of Pakistan, majority of which are edible. Around 310 thousand metric tons of fish production is appraised from inland waters of Pa…
14 Dec 2025DOI 10.17582/journal.pjz/20240414043202 - Research Article
Protective Effect of Melaleuca alternifolia Essential Oil on Broiler Growth Performance, Intestinal Morphology, Gut Microflora, and Gut Redox Status: An Alternative Growth Promoter to Antibiotics
Xiaofeng Zhang, Lihua Huang, Haiyan Ling, Liping Zhou
The effects of dietary Melaleuca alternifolia essential oil supplementation were investigated as an alternative to antibiotics on growth performance, intestinal health, and oxidative status in broilers. A total of 1960 yellow-skinned 1-day-old female broilers…
14 Dec 2025DOI 10.17582/journal.pjz/20240516020220 
Research ArticleNew Emerging Resistant Strains of Candida albicans Against Known Antifungal Drugs
Muhammad Zafar, Syeda Tahira Qousain Naqvi, Muhammad Imran Qadir, Muhammad Baber, Syed Bilal Hussain, Syed Nawazish-i-Husain et al.
Candida albicans, an opportunistic fungus, causing life-threatening infections in humans. Recently, there has been a profound increase in the prevalence of fungal resistance against antifungal drugs. Therefore, C. albicans isolated from clinical samples were i…
14 Dec 2025DOI 10.17582/journal.pjz/20201202131206
Research ArticleComparative Evaluation of Vincristine Sulfate and Vinblastine Sulfate for the Treatment of Transmissible Venereal Tumor in Dogs: A Pre-clinical and Pathological Study
Ishaq Ishaq, Huma Jamil, Muhammad Farooq, Qudrat Ullah, Muhammad Zahoor Khan, Rubina Mushtaq et al.
Canine Transmissible Venereal Tumor (TVT) is a sexually transmitted, contagious neoplasia in dogs. This study aimed to investigate the clinical and pathological characteristics of the TVT and assess the efficacy of two chemotherapeutic treatments, vincristine…
14 Dec 2025DOI 10.17582/journal.pjz/20230606060602
Research ArticleProbiotic and Antimicrobial Potential of Lactobacillus acidophilus Isolated from the Gut of Domestic Chicken
Gulmakai Shakoor, Ali Akbar, Habib Ur Rahman, Malalai Shakoor, Nazir Ahmad Khan
Probiotics are living microbes, beneficial to host when administered in sufficient amounts, known for their characteristics, such as no pathogenicity, tolerance to diverse environments, ability to colonise on mucosa of gastrointestinal tract and act as residen…
14 Dec 2025DOI 10.17582/journal.pjz/20240322041146
Research ArticleDiagnostic Approaches, Quantitative Assessment and Epidemiological Insights into Gastrointestinal Parasitism among Captive Peafowl (Pavo cristatus)
Sajida Arooj, Shabana Naz, Aniqa Qaisar, Anqash Ayub, Sania Satti, Alina Razzaq et al.
Gastrointestinal parasitism is one of the most prevalent and common sanitary issue that can lead to sub clinical infection or even death. For detection and quantitative investigation, direct smear procedure, fecal flotation method and McMaster technique was em…
29 Dec 2025DOI 10.17582/journal.pjz/20240802051304
Research ArticleComparative Analysis of Catch Efficiency and Seasonal Variations in Midwater Shark Longlining at Thengapattinam Fishing Harbour, Tamil Nadu
Archana Sethuraj, Neethiselvan Neethirajan, Padmavathy Pandurangan, Ravikumar Thangaraj, Uma Maheswari Thavasiyandi, Manikandan Boominathan et al.
This study evaluates the catch efficiency, vessel specifications, and seasonal variations of midwater longlining for sharks at Thengapattinam Fishing Harbour, (8.2341° N, 77.2991° E) Tamil Nadu. The research focuses on Type A (fiberglass, 18-20 m, 180-…
14 Dec 2025DOI 10.17582/journal.pjz/20250107110715
Research ArticleComparative Effectiveness of Repellents for Rose-Ringed Parakeet and House Crow at University of Agriculture, Faisalabad, Pakistan
Sajida Mustafa, Hammad Ahmad Khan, Sajid Abdullah, Muhammad Arshad
Present paper provides information on the comparative effectiveness of the three repellents: mist nets, reflecting ribbons and distress sound player with respect to the five economically significant crops viz. maize, chickpea, sugarcane and sunflower for the t…
14 Dec 2025DOI 10.17582/journal.pjz/20240516170136
Research ArticlePhytase as a Feed Additive: Impact on Growth, Nutrient Digestibility and Carcass Composition of Catla catla Fingerlings Fed on Different Plant By-Products Based Diets
Ambreen Sabir, Muhammad Zubair ul Hassan Arsalan, Tanveer Ahmed, Syed Makhdoom Hussain, Shafaqat Ali, Aumme Adeeba Bano et al.
Owing to existence of anti-nutritional factors (ANFs) such as phytate in diets based on plant by-products, fish show poor growth and nutrition retention in their bodies because less minerals and nutrients are available to them. To overcome this problem, phytas…
27 Dec 2025DOI 10.17582/journal.pjz/20240730064447
Research ArticleHost-Specific Biotic Potential of Small Brown Planthopper, Loadelphax striatellus (Hemiptera: Delphacidae); Life Table Analysis
Muhammad Rizwan, Misbah Rizwan, Bilal Atta, Arshad Makhdoom Sabir, Tahir H. Awan, Muhammad Arshad et al.
The small brown planthopper (SBPH), Laodelphax striatellus Fallén (Hemiptera: Delphacidae), is a very destructive insect pest that feeds on a variety of cereal crops, notably rice. We investigated the demographic parameters of SBPH by feeding on maize (…
29 Dec 2025DOI 10.17582/journal.pjz/20240408021712- Short Communication
Effects of Dietary Chitosan on Nutrient Intake and Digestibility of Peranakan Etawa (PE) Goat (Capra hircus Linn.)
Rakhmad Perkasa Harahap, Sadarman, Yeti Rohayeti, Nurwahid
This in vivo experiment was carried out to assess the impact of increasing amounts of chitosan in feed on dry matter intake (DMI), organic matter intake (OMI), crude protein intake (CPI), dry matter digestibility (DMD), organic matter digestibility (OMD), and…
2 Jan 2026DOI 10.17582/journal.pjz/20240730150849 
Short CommunicationThe Captive Roe Deer Successfully Bred Naturally after Being Released into the Wuling Mountain Nature Reserve in Beijing
Feng Gao, Xin Min Fang, Zhi Hong Ma, Dehuai Zhang
Reintroduction is an important wildlife conservation method, through artificial methods to reintroduce some endangered animals to their extinction places, form stable populations, and save endangered or extinct precious wildlife. In the past 20 years, reintrod…
3 Jan 2026DOI 10.17582/journal.pjz/20240927071750- Research Article
Antimicrobial Action of Olive Leaf (Olea europaea) Extract and its Interaction with Antibiotics in Salmonella gallinarum Infected Broiler Chicks
Shah Nawaz, Farzana Rizvi, Muhammad Asif, Muhammad Shahzad Shafiq, Ayesha Ramzan, M.Z. Shakir et al.
Ethanolic extract of olive (Olea europaea) leaves contains phenolic compounds; oleuropein and ligstroside. The current study was designed to investigate the antibacterial property of Olea europaea leaf extract (OLE) (orally @10ml/l) alone and in combination wi…
24 Nov 2025DOI 10.17582/journal.pjz/20231025071251 
Research ArticleBiodiversity of Marine Benthic Invertebrates in Port Qasim: Impacts of External Disturbances and Mangroves
Shahid Amjad, Jamshaid Iqbal, Muhammad Omar Arif Syed
From 2018 to 2022, a study was conducted in the navigational channel of Port Qasim to explore different taxonomic groups of marine benthic invertebrates (MBIs) across three different locations. The study focused on examining the distribution of nine common inv…
24 Nov 2025DOI 10.17582/journal.pjz/20240629073450- Research Article
Clinicomolecular Investigation of Bovine Rota and Coronaviruses and Their Association with Haematobiochemical Parameters in Diarrheic Cattle Calves
Safdar Abbas, Jawaria Ali Khan, Syed Saleem Ahmed, Aftab Ahmed Anjum, Nargis Ambreen, Sehrish Khan et al.
Neonatal calf diarrhea (NCD) is the most common cause of disease in newborn calves around the world. Pakistan’s young cattle population is severely affected by NCD caused by bovine rotavirus (BRoV) while bovine coronavirus (BCoV) outbreaks. BRoV is a dou…
24 Nov 2025DOI 10.17582/journal.pjz/20240718110524 
Research ArticleIncorporating Extruded Rapeseed Meal in Broiler Diets: Effects on Performance and Nutrient Absorption
Ateeb Shoukat, Muhammad Tahir, Shabana Naz, Rifat Ullah Khan, Ibrahim A. Alhidary, Saima Bibi et al.
This study investigates the utilization of extruded rapeseed meal (ERSM) in broiler diets and its effects on nutrient digestibility and growth performance. Two-hundred-and-forty-day-old broiler chicks were divided into four groups, including a control group wi…
14 Dec 2025DOI 10.17582/journal.pjz/20240609173400- Short Communication
Influence of Age and Body Condition Score on Body Conformation and Testicular Traits of Nguni Goats
Madumetja Cyril Mathapo, Salome Mhlabini, Thlaharani Cynthia Makamu, Thobela Louis Tyasi
The study was conducted to determine the effect of age and body condition score on body conformation and testicular traits using 20 Nguni goats. Bucks aged 1- 4 years were used. The body condition was scored on a scale of 1 to 5. The body linear measurements (…
3 Jan 2026DOI 10.17582/journal.pjz/20240117132100 - Research Article
Effect of Chilling, Freezing and Freeze-Thaw Cycles on Quality Characteristics of Goat Meat (Chevon)
Sambreena Tunio, Atta Hussain Shah, Muneer Ahmed Jamali, Ghulam Shabir Barham, Gul Bahar Khaskheli, Seema Gul Tunio et al.
Chevon is well-known for its abundant nutritional value and greater unsaturated to saturated fatty acid ratio. It is internationally regarded as a lean red meat with favorable nutritional characteristics. The influence of different times was observed on the ph…
20 Dec 2025DOI 10.17582/journal.pjz/20240129072937 
Research ArticleA Study on Interleukin-10 -1082 G/A (rs1800896) Polymorphism in Acute Lymphoblastic Leukemia Patients
Muhammad Ejaz, Imran Riaz Malik, Nasir Mahmood, Ghulam Mujtaba
In Pakistan, the prevalence of leukemia is 5.3% among all ages, while in children, the prevalence of acute lymphoblastic leukemia (ALL) is 19.8%. In the present study, 200 patients with acute lymphoblastic leukemia (n=200) and ethnically matched control sample…
27 Dec 2025DOI 10.17582/journal.pjz/20240107045910
Research ArticleComparison of Apoptosis Induction in Colon Cancer Cells According to Anticancer Drug Function
Min-Jee Oh, Baasanjav Batmunkh, Sang-Hwan Kim
In this study, we analyzed the differences in the induction of intrinsic apoptosis by camptothecin, rapamycin, paclitaxel, and vinblastine, which are representative anticancer drugs derived from plants or chemically recombinant substances, to control colon can…
27 Dec 2025DOI 10.17582/journal.pjz/20240807235139
Research ArticleEffect of Ammonium Chloride on Fermentation Quality and Bacterial Community of Distillers Grains Silage
Quanhui Peng, Ali Mujtaba Shah, Zhisheng Wang, Bai Xue, Lizhi Wang, Rui Hu et al.
The distillers grains (DGS) have high fiber, protein, and vitamins and are primarily fed to ruminants for maintenance and production. This study was conducted to investigate the effects of ammonium chloride on the fermentation quality and microbial dynamics of…
1 Jan 2026DOI 10.17582/journal.pjz/20230530080553
Short CommunicationHistopathological Changes in the Intestine of a Parrot (Melopsittacus undulatus) Naturally Infected with Cestode, Cotugnia karachiensis, from Karachi, Sindh, Pakistan
Rubab Malik, Nasira Khatoon, Mehtab Ibrahim
Cotugnia sp. is a parasitic tapeworm belonging to the class Cestoda and found in the gastrointestinal tract of various birds worldwide. The GIT tract is where food is digested, and parasites are normally observed due to a favourable environment for food and sh…
2 Jan 2026DOI 10.17582/journal.pjz/20240609185100
Research ArticleBetter Fitness of Sharpbelly, Hemiculter leucisculus (Basilewsky, 1855) in Invaded Habitats: Evidence from Body Length-Weight Relationships Across the World
Yuebing Zhou, Tao Ju, Lei Gan, Zhenlu Wang, Chao Luo, Weiwei Wang et al.
As one of the main driving factors of biodiversity loss, biological invasion has attracted wide attention. Among the many scientific issues in this field, the most noteworthy one is to figure out the ecological mechanisms behind successful invasions since rele…
20 Dec 2025DOI 10.17582/journal.pjz/20240420084908
Research ArticleEfficacy of Substituted Barley Meal Based Diet on Growth Performance, Nutrients Digestibility and Hematological Indices in Common Carp (Cyprinus carpio)
Muhammad Mudassar Shahzad, Syed Makhdoom Hussain, Kashifa Jalil, Fatima Yasin, Aasia Karim, Muhammad Asrar
With continuous expansion of human population, fish is a rich protein source used as food all over the world but high cost of fish meal (FM) and its inconsistent supply has entailed researchers to find its substitute. In present experimental work, plant-based…
1 Jan 2026DOI 10.17582/journal.pjz/20241019061018- Review
An Overview of Insect Mite Association
Allwin Loveson, Vaithiyanathan Radhakrishnan, S. Vijay, Varadharajan, Baskaran, Madhaiyan Ravi et al.
Mite comprises the largest group within the arthropodan of Class Arachnida, with over 45,000 described species in subclass Acari. The Arachnida is also represented by the spiders (Order: Araneae), harvestmen (Order: Opiliones) and pseudoscorpions (Order: Pseud…
2 Jan 2026DOI 10.17582/journal.pjz/20240816085733 
Research ArticleContribution and Diversity of Integrons and acrAB-TolC Efflux Pump to Multidrug Resistance in Clinical Isolates of Escherichia coli Isolated from Pakistan and China
Muhammad Yaqoob, Li Ping Wang, Shaohui Wang, Javed Memon, Lu Cheng-Ping
The contribution and diversity of integrons and efflux pump to multidrug resistance in avian Escherichia coli, isolated from Pakistan and China, was evaluated. Real time RT-PCR, RT-PCR and direct sequencing methods were used to detect the presence of potential…
27 Dec 2025DOI 10.17582/journal.pjz/20210323110345
Research ArticleEffects of Substituted Blackseed Meal Based Diet on Growth Performance and Nutrient Digestibility of Cyprinus carpio Fingerlings
Muhammad Mudassar Shahzad, Imran Farooq, Muhammad Tariq, Zawar Hussain, Fatima Yasin, Muhammad Usman
Black seeds (Nigella sativa) and their derivatives have certain beneficial consequences for farmed fish. Because of its great adaptability in diet, it is being investigated as a prospective option for commercial aquaculture feed in Asia. To check the effects o…
29 Dec 2025DOI 10.17582/journal.pjz/20220630060617
Research ArticleDietary Organic Selenium Supplementation Improved Some Histo-Morphological Features and Stimulated G1 Cell Cycle Progression in Testis of Pre-Pubertal Male Goat
Muhammad Awais Soomro, Moolchand Malhi, Allah Bux Kachiwal, Asmatullah Kaka, Saeed Ahmed Soomro, Jamila Soomro
Selenium (Se) nutrition improves testicular growth in young ruminants. We speculated that Se supplementation would modulate proliferative gene expression and improve morphological characteristics in testes, which eventually result in pre-pubertal testicular gr…
21 Dec 2025DOI 10.17582/journal.pjz/20240503083935
Short CommunicationPhylogenetic Analysis of E6 and E7 Gene of Human Papillomavirus 16 Variants Isolated from Pakistani Population
Gulshan Zaidi, Sameen Ahmed, Ayesha Vajeeha, Muhammad Idrees, Shazia Rafique
HR-HPVs, or high-risk human papillomaviruses, are the cause of cervical cancer, ranks as the second leading cause of female mortality worldwide, following breast cancer. HPV16 is globally recognized as the most common high-risk type. This research sought to ex…
2 Jan 2026DOI 10.17582/journal.pjz/20220628070610
Research ArticleArthospira platensis: Growth and Biochemical Composition on Various Combinations of Fish Waste Silage and Seaweed Hydrolysate
C. Rajeswari, D. Manikandavelu, B. Ahilan, S. Aruna, N. Muralidharan, P. Ruby
Spirulina is a single-cell protein rich in all essential nutrients and vitamins and can be used to produce functional food. This study was conducted to evolve suitable organic media for algal culture by evaluating various combinations of fish waste and seaweed…
1 Jan 2026DOI 10.17582/journal.pjz/20240610062151
Research ArticleMammalian Cell Expression of a Fully Functional Recombinant E6 Protein Encoded by HPV16 Isolate from Pakistani Population
Ayesha Vajeeha, Shazia Rafique, Sameen Ahmed, Muhammad Idrees, Rakhtasha Munir, Gulshan Zaidi et al.
Human papillomavirus type 16 (HPV16) accounts for about 65% of the total cervical cancer burden. Viral oncoprotein E6 is an ideal target for immunotherapeutic strategies against HPV induced cervical cancers, as it is constitutively expressed during the d…
1 Jan 2026DOI 10.17582/journal.pjz/20221116071159
Short CommunicationThe Complete Mitochondrial Genome of Leopard Cat, Prionailurus bengalensis euptilurus (Carnivora:Felidae)
Feng Gao, Jinping Zhang
The full-length of the mitochondrial (mt) genome of leopard cat (Prionailurus bengalensis euptilurus) was first determined in this study and consisted of a 17,246bp fragment , including 13 protein-coding genes, two rRNA genes, 22 tRNA genes, a control region (…
3 Jan 2026DOI 10.17582/journal.pjz/20240728093513- Research Article
Toxicity Effects of Green Synthesized Zinc Oxide Nanoparticles on Hematology and Biochemical Parameters in Cirrhinus mrigala
Khalid Javed Iqbal, Hamid Majeed, Noor Khan, Tahreem Kousar, Muhammad Asghar, Khalid Jamshed Iqbal et al.
Current research was aimed to analyse the toxic effect of ZnO-NPs on hematology and serum biochemistry parameters in Cirrhinus mrigala with a post-exposure period of 96 h and 15 days. The experimental fish were divided into four groups (10 fish per group) i.e.…
27 Dec 2025DOI 10.17582/journal.pjz/20220611150626 
Research ArticleAntibiogram and Bacterial Profiling of Reproductive Tract Infections in Female Cattle and Buffaloes
Abdul Jabar, Ishtiaq Ahmed, Aziz ur Rehman, Muhammad Zishan Ahmad, Muhammad Kamran Rafique, Asim Aslam et al.
Reproductive tract disorders are considered a major cause of low conception rate and poor productivity of livestock, leading to infertility or sterility of animals, and thus, results in high economic losses. The current study was designed to investigate the ro…
27 Dec 2025DOI 10.17582/journal.pjz/20230409130421
Short CommunicationDiversity of Freshwater Gastropoda Snails Found in District Swat, Khyber Pakhtunkhwa, Pakistan
Shah Zeb Ahmad, Sadaf Niaz, Akhtar Rasool, Naseem Rafiq, Chen Zhi
Mollusks a group of macroinvertebrates found in both aquatic and freshwater habitats are the oldest animals. Historically which goes back to Cambrian deposits. They have got a second rank in abundance after the more abundant arthropods taxon and are the most i…
2 Jan 2026DOI 10.17582/journal.pjz/20240724084703
Research ArticleEfficacy of Garlic Extract on the Reproductive Parameters of Root Knot Nematode, Meloidogyne incognita (Kofoid and White) Chitwood
Ayesha Javed, Huma Abbas, Sajid Aleem Khan, Maham Batool, Aqsa Ashfaq, Muhammad Kamran et al.
The present study aimed to assess the efficacy of garlic aqueous extract (GAE) on the reproduction of the root knot nematode (RKN), Meloidogyne incognita, in tomato plants. Four experiments were conducted during this investigation using different concentration…
27 Dec 2025DOI 10.17582/journal.pjz/20220929050919
Research ArticleA Systematic Survey of Alpha Diversity of Hymenopterans in Maize and Mustard Cash Crops of the Arid Region of Punjab, Pakistan
Aftab Raza Khan, Muhammad Khalid Mukhtar, Azhar Abbas Khan, Zeshan Hassan, Tahira Abbas, Fazeela Saleem et al.
Insects as pollinators are vital components for long-term sustainability and proper functioning of agroecosystems. Comprehensive investigations were undertaken to observe the alpha diversity and status of hymenoptera pollinators in relation to cash crop floris…
27 Dec 2025DOI 10.17582/journal.pjz/20240805070354
Research ArticleCharacterization and Expression Analysis of the Detoxification Enzyme Gene GSTd3 in Bombyx mandarina
En-Xi Chen, Ruo-Nan Li, Meng-Jiao Wang, Lin Zhu, Yu-Ming Zhang, Yi-Xuan Qian et al.
Bombyx mandarina is one of the major pests in mulberry gardens, and because of its overlapping generations and leaf-rolling habit, it brings great difficulties to the control. At present, the control of B. mandarina is mainly by chemical means, but the massive…
27 Dec 2025DOI 10.17582/journal.pjz/20240319080355- Research Article
Genetic Characterization and Identification of Pakistani Avian Fauna through DNA Barcoding
Muhammad Zia ul Haq, Sehrish Firyal, Aftab Ahmad Anjum, Muhammad Wasim, Rana Manzoor Ahmad, Maryem Hussain et al.
Bird species that are difficult to identify taxonomically using morphological methods remain a challenge for ornithologists in accurate species identification. International migratory bird route number 4 (Indus Flyway) traverse through Pakistan. Current study…
14 Dec 2025DOI 10.17582/journal.pjz/20240323121422 
Research ArticleDevelopment and Evaluation of Inactivated Whole-Cell Contagious Caprine Pleuropneumonia Vaccine Against Mycoplasma capricolum Subsp. capripneumoniae Pakistan Strain
Hayatullah Khan, Farhan Anwar Khan, Umer Sadique, Ijaz Ahmad, Faisal Ahmad, Hanif Ur Rahman et al.
To control and prevent various types of infectious diseases that affect the animal population, vaccination is a simple and efficient solution. The goat population suffers greatly from the serious respiratory disease contagious caprine pleuropneumonia (CCPP). B…
1 Jan 2026DOI 10.17582/journal.pjz/20240114191217
Research ArticleAntimicrobial Action of Olive Leaf (Olea europaea) Extract and its Interaction with Antibiotics in Salmonella gallinarum Infected Broiler Chicks
Shah Nawaz, Farzana Rizvi, Muhammad Asif, Muhammad Shahzad Shafiq, Ayesha Ramzan, M.Z. Shakir et al.
Ethanolic extract of olive (Olea europaea) leaves contains phenolic compounds; oleuropein and ligstroside. The current study was designed to investigate the antibacterial property of Olea europaea leaf extract (OLE) (orally @10ml/l) alone and in combination wi…
24 Nov 2025DOI 10.17582/journal.pjz/20231025071251
Short CommunicationDevelopment of Microsatellite Markers for Three Schizothorax Species: S. macropogon, S. oconnori and S. waltoni
Haiqing Yang, Lei Wu, Guanfeng Jin, Yong Wang, Wei Liu, Qinwen Tan et al.
Schizothorax spp. has experienced a rapid decline in wild population size. Evaluating its genetic diversity and population genetic structure is of critical importance. We developed ten microsatelittes for S. macropogon, S. oconnori, and S. waltoni, which will…
2 Jan 2026DOI 10.17582/journal.pjz/20240716141648
Research ArticleMolecular Characterization of a Thermostable L-Asparaginase from Geobacillus SBS-4S
Barizah Malik, Hina Khalid, Zartaj Zafar, Saleha Akhtar, Arooj Fatima, Arooba Tariq et al.
L-Asparaginase enzyme is known for its utilization in the treatment of acute lymphoblastic leukemia (ALL) and in the food industry as it depletes acrylamide which is a carcinogen often produced in baked foods. L-Asparaginase enzyme hydrolyzes L-asparagine into…
1 Jan 2026DOI 10.17582/journal.pjz/20240531082915
Short CommunicationGenome Survey and Microsatellite Motifs Analysis of a Marine Fish, Argyrosomus japonicus
Tianxiang Gao, Tao Liu, Bingjie Chen, Xinxin Huang, Tianyan Yang, Qi Liu et al.
Argyrosomus japonicus is an economically important marine fish. However, the wild stocks of A. japonicus are declining due to overfishing. Recently the large-scale stock enhancement of A. japonicus was carried out in China. Here we reported the basic informati…
3 Jan 2026DOI 10.17582/journal.pjz/20221207021205
Research ArticleDietary Selenium Supplementation Reduces Oxidative Stress and Attenuates Apoptotic Gene Expression in Testes of Pre-Pubertal Male Goat
Muhammad Awais Soomro, Moolchand Malhi, Allah Bux Kachiwal, Asmatullah Kaka, Saba Parveen Samo, Tarique Ahmed Khokhar et al.
Selenium (Se) nutrition improves reproductive health in ruminants. We hypothesized that pre-pubertal Se supplementation in feed would improve testicular growth by modulating oxidative stress (OS) and apoptosis. To test this hypothesis, twenty young cross-bred…
29 Dec 2025DOI 10.17582/journal.pjz/20240731090049
Research ArticleAntibiotic Resistance and Antibiotic Resistance Genes in Staphylococcus aureus Isolates from Hospital Patients in Islamabad, Pakistan
Saima Batool, Waqar Saleem, Khurshid Ahmad, Saeed-ul-Hassan Khan
Two hundred and seventy-eight clinical samples from hospital patients including pus, blood, nasal swabs, ear swabs, urethral swabs, wounds and sputum were processed for isolation of S. aureus. From these samples 50 S. aureus isolates were recovered, which were…
27 Dec 2025DOI 10.17582/journal.pjz/20200423150456
Research ArticleCowine, an Organic Adjuvant from Lactic Acid Bacteria-Rich Edible Commodities in Suppressing the Sucking Pests of Rice (Oryza sativa)
Loveson Allwin, Sundararaj Susikaran, Radhakrishnan Vaithiyanathan, Chandrasekaran Harisudan, Paramasivan Muthiah, Maria Anthonymuthu Ilangovan Manivannan et al.
Adjuvants are often mixed with insecticides during formulating or spraying. Resurgence of sucking pests follows pesticide application in rice. Increased tank mixing of most potent adjuvants such as organosilicone surfactants has been associated with declining…
21 Dec 2025DOI 10.17582/journal.pjz/20240427052801
Research ArticleSeasonal Diversity of Planktonic Ciliates in Relation to Environmental Variables from Coastal Waters of Pakistan (Northern Arabian Sea)
Roomana Yasmeen, Nafisa Shoaib, Tayyaba Hamid
Ciliates are an essential component of the microzooplankton and occupy a significant role in the microbial food web. In the present study, seawater samples were collected in four seasons from the Gadani shipbreaking area and Sandspit coasts for one year. The s…
20 Dec 2025DOI 10.17582/journal.pjz/20221221151233- Research Article
Species Diversity, Existence of Virulence Gene Profile and in-vivo Pathogenicity Study of Aeromonas spp. Isolated from Diseased Catla catla
Chethurajupalli Lavanya, Tambireddy Neeraja, Thatavarthi Venkata Ramana, Arumugam Balasubramanian, Ogirala Sudhakar
This study aimed to isolate Aeromonas species from hemorrhagic septicemia diseased Catla catla freshwater fish in Andhra Pradesh, India, in order to learn more about them, locate its virulence genes, and evaluate their pathogenicity. A total of nine Aeromonas…
14 Dec 2025DOI 10.17582/journal.pjz/20240525064332 
Research ArticleMolecular Detection of Salmonella enterica Serovar Gallinarum, Biovar Gallinarum and Biovar Pullorum from Poultry Birds (Gallus gallus domesticus) in Faisalabad, Pakistan
Mariya Azam, Muhammad Adnan Ashraf, Ahsan Javed, Muhammad Kashif Saleemi, Ikram ul Haq, Yasra Sarwar et al.
Salmonella enterica sub-species enterica serovar Gallinarum, biovar Gallinarum (S. Gallinarum/bvSG) and biovar Pullorum (S. Pullorum/bvSP) are the etiologic agents of fowl typhoid (FT) and pullorum disease (PD), respectively. A limited number of studies from S…
14 Dec 2025DOI 10.17582/journal.pjz/20240323132852
Research ArticleDynamics of Tissues Macro-Minerals Changes of Growing Camels (Camelus dromedarius) in Spring Season of Saudi Arabia
Mutassim M. Abdelrahman, Ibrahim A. Alhidary, Mohammed A. Al-Badwi, Mohsen M. Alobre, Abdulkareem M. Matar, Riyadh S. Aljumaah
The aim of our study was to evaluate the calcium (Ca), phosphorus (P), and magnesium (Mg) status of slaughtered camel tissues in five regions in Saudi Arabia during the spring season. Selected tissues (whole blood, liver, meat, rumen tissue and rumen fluid sam…
24 Nov 2025DOI 10.17582/journal.pjz/20240407162841
Research ArticleEffect of Hi-Pro Corn Distiller’s Dried Grains with Solubles (DDGS) and Commonly Used Vegetable Protein Sources on Growth Performance, Amino Acid Digestibility and Intestinal Health in Broilers
Mubashar Hussain, Muhammad Saeed, Muhammad Aslam Mirza, Fawwad Ahmad, Asghar Ali Kamboh
The trial was carried out to compare the effect of Hi-Pro Distiller’s dried grains with solubles (DDGS) with conventionally available vegetable protein ingredients such as soybean meal (SBM), sunflower meal (SFM), canola meal (CM) and rapeseed meal (RSM)…
24 Nov 2025DOI 10.17582/journal.pjz/20240120134816