Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...ine, macrolides and aminoglycosides. No resistance was observed against vancomycin and linezolid. Among genes involved in host immune evasion, Staphylococcus protein A (spa) was identified most frequently (81%) and proportions of capsular polysaccharides (CPs8), clumping factor A (clf A) andintracellular adhesion A (ica A) were 78%, 68.5% and 40%, respectively. ica D and CPs5 could not be amplified from any isol...
Nadia Khan1*, Abdul Waheed1, Farrukh Siyar Hamid1, Naveed Ahmed1, Zafar Iqbal2, Seemab Ali1, Madiha Bashir1 and Hina Gul3
...lkaloids, flavonoids and glycosides. To test the antibacterial activity of the extracted seed oil, Agar well diffusion assay was employed. Effectiveness of the oil against selected bacterial species was measured in terms of zone of inhibition in millimeters. Amoxicillin was used as a positive control. The results showed that Clavibacter sp. and E. coli exhibit resistant to the oil extract while Xanthomonas sp. displayed nil results. The present study thus reve...
Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*
...loids, saponins, cardiac glycosides, tannins, phenols and flavonoids etc. It is concluded that S. saligna crude extracts as well as crude saponins exhibit broad antimicrobial spectrum against various disease-causing microbes. ...
Gregory Ejikeme Odo1,*, Juliana Ekenma Agwu1, Nkechi Nweze2, Clara Ikegbunam2,Felicia Ekeh N1, Vincent Ejere1, Ngozi Ezenwaji1, Reginald Njokuocha2, Godwin Ngwu1 and Fabian Okafor1
...ents such as the cardiac glycosides, steroids, saponins, and tannins are moderate in abundance (other constituents such as the cardiac glycosides, steroids, saponins, and tannins that were moderate in abundance (++). There was a dose duration dependent (p < 0.05) decrease in RBC in all the groups. There were also duration-dependent decrease in MCV values obtained in this research as against duration and dose dependent inc...
Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5
Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...align: justify;">Steviol glycosides contained in Stevia leaves have considerable potential uses for the natural sweetener industry due to it’s antidiabetic properties, hence a large number of plant source is required to supply the industry. To overcome the hindrances in the propagation of Stevia plants using in vivo methods, an alternative technique of propagation using in vitro methods is needed. The micropropagation method with the RITA® (Recipient...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
...uoroquinolones, and aminoglycosides. This study investigates the high prevalence of ESBL producing E. coli in chicken, farmworkers, and water that is alarming and could lead to serious threats to both livestock and public health.
...
Shahida Sabir1, Anwar Ali Shad1, Tariq Masood1*, Zafar Ali Shah1, Nasiruddin2, Yaseen Ahmad1 and Syed Sartaj Alam3
..., lipids, terpenoids and glycosides were present in extracts at moderate to high concentrations. Methanolic fraction of mushroom proved to be bactericidal with highest % inhibition (65, 36 and 75 %) recorded at 25 mg/L concentration against all the three bacterial strains i.e., E. coli, Staphylococcus aureus and Xanthomonas sp., respectively. Maximum % inhibition (about 50%) was recorded at 25 mg/L concentration against all the three fungal species (Penicilliu...
Muhammad Noman Koondhar1, Asghar Ali Kamboh1,*, Muhammad Ammar Khan2, Riaz Ahmed Leghari3 and Parkash Dewani4
...%), followed by the aminoglycosides (68.4 to 85.5%), tetracycline (73.9 to 78.6%), and quinolones (59.6 to 63.1%). While lowest resistance level was observed against the antibiotics of cephalosporin group (32.1 to 52.6%). Among 13 isolates of S. Pullorum, a highest number (10 isolates) showed resistance against neomycin, kanamycin and tetracycline. A total of 76.9% (10/13) isolates were recorded as multidrug resistant (MDR) resistant. Maximum num...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
... the routinely used aminoglycosides in clinical settings. The current study aimed to screen the armA genes among the A. baumannii isolates collected from clinical specimens at the tertiary care hospitals of Lahore and Faisalabad,Pakistan. A total of 148 A. baumannii isolates were collected. The initial identification of bacterial isolates was performed by standard microbiological techniques, and API 20E and for final confirmation multiplex...
Saima Rubab1,3*, Ghazala H Rizwani2, Arjumand Iqbal Durrani3, Iram Liaqat4*, Urooj Zafar5, Mahjabeen6, Farah Batool7, Noor-E- Seher3, Naveera Younas3 and Ayesha Sadiqa8
..., flavonoids, alkaloids, glycosides and carbohydrates in all plant parts with varied strength. Phytochemical analysis showed comparatively high percentage yield of ethanolic extract of all plant parts, hence was employed for evaluating the antimicrobial potential against 11 Gram positive bacteria, 9 Gram negative bacteria, 2 yeasts, 2 dermatophytes and 7 saprophytes. The petroleum ether seed extract and methanolic leaf extract was evaluated for the comparative...
O. A. Fabiyi
...ars, phenols, alkaloids, glycosides and carbohydrates. Nematicidal activity of D. oliveri
leaves is being reported for the first time. The crude extracts and chromatographic fractions are potent at very low
concentrations compared with standard carbofuran. Therefore, the leaves of D. oliveri could be employed as a
viable source of natural nematicide instead of synthetic toxic nematicides.
...
Yang Hu, Jingxin Ding, Beilei Xu, Xiangming Sun, Guoyu Li, Hui Song, Zheng Zong and Wenlan Li*
...s active ingredients are glycosides. To explore the absorbed constituents of total glycosides (TGs) in vivo and its estrogen-like activity, and reveal its direct acting substances in the body, in this study, 30 female Wistar rats were orally administered with TGs, and serum fingerprints of blood samples collected at 10 different times were established by UPLC-Q-TOF-MS analysis. The estrogen-like activity of TGs was evaluated...
ZAMARA MASOOD1, FAROOQ SALEEM1, SARFRAZ AHMAD2, TALHA JAMSHAID3, IMRAN WAHEED4,
MUHAMMAD AZEEM1, UMAR FAROOQ GOHAR5 & HAFIZ MUHAMMAD ARSALAN6, 7*
...e="text-align: justify;">glycosides, cardiac glycosides, saponins, flavonoids, cumarins, steroids,
proteins, and amino acids, fixed fats and oils in all fractions. Floral
extracts were found to contain highest total phenolic contents (64 to 73
mg GAE/g), whereas leaves n-hexane fraction exhibited maximum DPPH
potential (70 %). All the extracts showed promising antimicrobial act...
Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1
...flavonoids, tannins, and glycosides. The test organism was more sensitive to C. limonum compared to C. aurantium. The MICs ranged from 1.56 to 12.5mg/ml; MBCs, (3.125 - 25mg/ml) and MBC/MIC ratios (2.0-4.0). Furthermore, a time killing curve of each 4x MICs and 8x MICs concentrations of juice extract showed a distinguished bactericidal effect at 6th hr. Therefore, the use of citrus fruit juice as medicine to treat infections is supported scientifically by thes...
Sara H. Zughayyar*, Amer H. Gyad
...coumarins, terpenes, and glycosides, while steroids and proteins were absent. The extract displayed antidiarrheal activity comparable to loperamide, showing a significant reduction in intestinal content volume and weight (P≤0.05). In conclusion, the ethanolic extract of Prosopis farcta L. fruits demonstrated promising antidiarrheal activity, potentially due to the phytochemical constituents identified, warranting further research for clinical applications.&...
ASIFA BASHIR1, AAMIR MUSHTAQ2* & TOOBA MEHBOOB3
...s, flavonoids, saponins, glycosides, amino acids and proteins in crude extract with phenolic and flavonoid contents observed as 9.9 mg/g equivalent of gallic acid and 100 mg/g equivalent of rutin, respectively. Free radical scavenging activity by plant extract was observed as 68±0.33 % as compared to rutin (58±1.15 %) at concentration 2.56 mg/ml. Similarly, in vivo studies indicated that Phyllanthus emblica 80 mg/kg significantly reduced the gluc...
Shah Zeb Khan1, Ali Hazrat1*, Farman Ali Khan2, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar3, Muhammad Ajmal Khan4 and Hayat Ullah1
..., terpenoids, alkaloids, glycosides, and saponins) in the bark of V. negundo (L.) roots and stem, as well as their potential antioxidant and antibacterial properties. The crude was obtained in 95 % methanolic which was further fractionated into n-hexane (HR, HS), Chloroform (CR, CS), ethyl acetate (EtR, EtS) and butanol (BR, BS) soluble fractions by solvent-solvent extraction. In phytochemical tests of roots fractions; HR showed the presence of terpenoids, whi...
Kingsley O. Omeje1, Juliet N. Ozioko2, Amaechi L. Ogara2, Benjamin O. Ezema2* and Dilibe C. Urama3
...lavonoids (3.62 mg/QEq), glycosides (0.194 mg/100g), tannins (8.508mg/TAEq) and alkaloids (6.43%) were quantified. There was no sign of mortality or toxicity after the acute and chronic toxicity tests after 24 hr. Some hematological indices (red blood, white blood cells, packed cell volume, lymphocytes, neutrophil, mean cell volume) increased after the 28-day treatment regime. There was increase in the plasma concentration of high-density lipoprotein and triac...
Zara Naeem1, Khajista Jabeen1*, Muhammad Khalid Saeed2, Sumera Iqbal1
...oids, phlobatannins, and glycosides. Hence it can be concluded that the methanolic leaf extract of the tested plant effectively inhibited the growth of test mycotoxigenic fungi....
Azhar Ali1, Faheem Ahmed Khan2, Wu Di3, Huang Chunjie3, Muhammad Rizwan Yousaf1, Bilal Ahmed1, Farwa Shakeel1, Nuruliarizki Shinta Pandupuspitasari1, Windu Negara2, Bambang Waluyo Hadi Eko Prasetiyono1*
...β-lactams, and aminoglycosides, which correspond to antibiotics often administered to both animals and humans. An important discovery in this study is the recognition of Enterobacteriaceae as the predominant family in the phylum Proteobacteria, together with enterococcus species from the phylum Firmicutes, which possess numerous antibiotic resistance genes associated with health risks. The presence of antibiotic-resistant Gram-negative and Gram-positive b...
