Guang-Xin E1, Yong-Ju Zhao1, Zhong-Yuan Zhang1, Yan-Guo Han1, Wei-Wei Ni1, Ming-Xing Chu2, Yue-Hui Ma2, Jian-Ning He3, Huai-Zhi Jiang4, Jia-Hua Zhang1, Zhong-Quan Zhao1, Yan Zeng1, Ri-Su Na1, Gao-Fu Wang5, Hang-Xing Ren5 and Yong-Fu Huang1*
...y domestic animals. Some microsatellites related to the fecundity of domestic animals have been identified. In the current study, we investigated five fecundity related microsatellites, including BM1329, BM143, LSCV043, OarHH35, and OarHH55, which had been identified in sheep. We estimated their diversity and population structure in three high fecundity goats (Jianzhou big ear goat, Jining grey goat, Dazu black goat) and thr...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,*
...a), we developed six microsatellite markers using a method of fast isolation by AFLP of sequences containing repeats (FIASCO) from them in this study. The primers designed by Primer Premier 5.0 based on conservative regions were tested on 22 individuals. Our results demonstrated that the number of alleles per locus ranged from two to five and the mean number of alleles was 3.67. The observed and expected heterozygosities ranged from 0.227 to 0.545 and 0.20...
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
...y of wild population, 11 microsatellite loci were firstly used to evaluate the genetic characterization using 120 samples from 2 tributaries (ZZ and LX populations) of the Weihe river and 2 tributaries (YX and TB population) of Hanjiang river. A total of 59 alleles were found and the number of alleles in each locus ranged from 3 to 8, with an average of 5.4. There were 6 highly polymorphic loci and 4 moderately polymorphic loci. The average expected heterozygo...
Lin Wang1,2, Ye Gong3, Kelin Chen1, Haitao Wang3 and Xianguo Lyu1,*
...cies amplification of 90 microsatellite loci developed for eight other species. Eleven out of them were successfully amplified and polymorphic with 2-10 alleles. The observed heterozygosities ranged from 0.481 to 0.827 and the polymorphic information content ranged from 0.373 to 0.775. Significant linkage disequilibrium was found only between two markers. These microsatellites could be used to enhance our understanding of ge...
Qin Liu1,2, Feiwei Liu1, Xiuyue Zhang1, Nan Yang1 and Jianghong Ran1,*
...ymorphic tetranucleotide microsatellite markers were isolated from T. szechenyii using a next-generation sequencing technology. The allele number of these loci ranged from two to six in genotyped 35 individuals. Polymorphism information content ranged from 0.2735 to 0.7717 with an average of 0.5149. Observed and expected heterozygosities at each locus ranged from 0 to 0.879 and 0.332 to 0.814, respectively. These markers could be used to better understa...
Zeqin Fu, Yunfang Tian, Yingying Ye*, Pengzhi Qi and Changwen Wu
...mers pairs to screen all microsatellite loci and twenty-three polymorphic loci were identified. The number of alleles per loci ranged from six to twenty-one with an average of 12.913 and the allele size varied between 161 and 470 bp. The observed and expected heterozygosity varied from 0.143 to 1.000 and from 0.351 to 0.926, with average values of 0.614 and 0.771, respectively. Eighteen loci accorded with the Hardy-Weinberg Equilibrium (HWE)(P &g...
Xiangbin Meng1,Tianxiang Gao2, Chunhou Li3, Na Song1, Lu Liu1, Liqin Liu2 and Xiumei Zhang1,*
... obtain potential useful microsatellite markers for S. esculenta. Among 80 tested microsatellite markers, 19 showed polymorphism among S. esculenta individuals. The allele number of all polymorphic microsatellite markers ranged from 6 to 10. Expected and observed heterozygosity varied from 0.260 to 0.904 and from 0.292 to 0.861, with an average of 0.741 and 0.607, respectivel...
Liqin Liu, Maoting Wang, Zhenming Lü, Li Gong*, ChangWen Wu, Baoying Guo and Lihua Jiang
... we developed twenty-one microsatellite loci for S. pharaonis through next-generation sequencing technology. A total of 100 alleles were detected, and the number of alleles per loci ranged from 2 to 9. The observed and expected heterozygosities per loci ranged from 0.000 to 0.531 and from 0.031 to 0.751, respectively. Polymorphism information content (PIC) showed that six loci were highly informative (PIC > 0.5). Five loci (CL1142, CL1770, CL2683, CL...
Jie Zhang1,2,*, Baradi Waryani3,4 and Qihai Zhou2,*
...n details 12 polymorphic microsatellite loci and 12 to 26 alleles per locus denoted. Whereas, d expected and observed heterozygosity values ranged from 0.902 to 0.972 and 0.750 to 0.958, respectively. Moreover, the polymorphic information content (PIC) values ranged from 0.869 to 0.949. The cross-species amplification and applicability were also evaluated on Protosalanx chinensis, Neosalanx anderssoni, Neosalanx argentea and Neosalanx o...
Lusha Liu1,*, Lei Cheng2, Xingqi Zhang3, Yulong Li1 and Jianping Jiang1,*
... But the availability of microsatellites especially from expressed sequences is currently very limited in this species and the genus Microhyla. A total of 17,339 potential microsatellites were identified in 15,385 unigenes which were generated by Illumina paired-end sequencing in M. fissipes. Within all microsatellites, AG/CT, AAG/CTT, and AAAG/CTTT are most prevalent motif i...
Yan-Bin Zhu1, Wang-Dui Basang1, Zhan-Dui Pingcuo1, Yang-Ji Cidan1, Sang Luo1, Dun-Zhu Luosang1, Yang-La Dawa1 and Guang-Xin E2,3,*
...ulation using twenty-one microsatellites. The results revealed that the HO ranged from 0.4854±0.0194 in NNV to 0.6086±0.0267 in YRY, and the NA ranged from 3.86±1.98 in XMY to 6.05±3.37 in NNV. The least number of markers which deviated from Hardy-Weinberg equilibrium within the population was W, and largest number of deviations was in the NNV population. Consistent of phylogenetic relationships between the seven populations was ide...
Yacheng Hu1,2, Xueqing Liu1,2, Jing Yang1,2, Kan Xiao1,2, Binzhong Wang1,2 and Hejun Du1,2,*
...t 17 novel cross-species microsatellite markers for Dabry’s sturgeon via next-generation sequencing from Chinese sturgeon (Acipenser sinensis). The 17 microsatellites were polymorphic with 3 to 6 alleles per locus and the total number of alleles is 80. The mean expected heterozygosity (HE, 0.506 to 0.781), observed heterozygosity (HO, 0.542 to 1), Hardy-Weinberg departure valu...
Shanshan Cai1, Tianxiang Gao2, Binlun Yan3, Aiyi Zhu1 and Xiumei Zhang1,2,*
...gion fragments and three microsatellite markers. When using mtDNA loci, 242 individuals (44.24%) were excluded for no shared haplotype with broodstock. Further 81 (14.81%) crabs were identified as hatchery-reared individuals based on microsatellite loci from the remaining 305 crabs. Our results also showed high genetic diversity and a certain degree of heterozygote deficiency of natural swimming crab populations. The advanci...
Jiaxin Guo1, Xiumei Zhang1,2, Xiaoyan Wang2, Liqin Liu2 and Tianxiang Gao1,*
...sed for potential useful microsatellite markers obtaining of S. japonica. A total of 120 microsatellite markers were designed and tested, 25 primer pairs showed polymorphism among S. japonica individuals. Number of alleles, observed and expected heterozygosity per locus ranged from 2 to 8, 0.083 to 0.922 and 0.042 to 0.866, respectively. The PIC ranged from 0.04 to 0.829. These markers will be useful in evaluat...
Qingzhan Ma1, Bo Wu1, Jianping Jiang2 and Zhaobin Song1,3*
...tificial breeding. Eight microsatellite markers were used to analyze genetic characteristics of eight channel catfish populations (POPA, POPB, POPC, POPD, POPE, POPF, POPG, and POPH). The average polymorphic information content (PIC) ranged from 0.525 (POPD) to 0.733 (POPA) indicating high polymorphism (PIC > 0.5). The average observed heterozygosity (Ho) and expected heterozygosity (He) in the eight populations ranged from 0.504 (POPH) to 0.7...
Jianjun Fu1, Shili Liu2, 3, Wenbin Zhu1, Yongyi Jia2, 3, Lanmei Wang1, Yufen Yang4, Zhimin Gu2, 3*and Zaijie Dong1*
...panels, including twelve microsatellite loci for C. alburnus, and applied parentage analyses using 36 candidate parents and 136 offspring from four independent artificial breeding pilots. Based on allele frequency analysis using 36 candidate parents, the number of alleles ranged from 7 to 22, observed heterozygosity ranged from 0.39 to 0.75, and expected heterozygosity ranged from 0.57 to 0.91. All loci were highly informative (polymorphic information c...
Jun Yan Bai1*, Ren Tao Di Wu2, Qiang Zhang2, Di Bao2, Le Ma Dao2 and Xing Hua Tian3
...mall;">In this study, 17 microsatellite markers were used to analyze the genetic diversity of Sonid bactrian camels, and the correlation between microsatellite markers and their body size was analyzed to find microsatellite markers associated with the body size traits of Sonid bactrian camels, so as to provide reference for marker-assisted selection of Sonid bactrian camels. The results sh...
Qin Liu1,2, Bin Wang1,3, Yu Xu1,4, Xiuyue Zhang1, Tao Zeng1* and Jianghong Ran1*
...ated 17 tetra-nucleotide microsatellite loci to test parentage and kinship for a wild population with 29 individuals from 10 different families (A-J). The 17 loci with the mean polymorphic information content (PIC) of 0.566 and the combined probability of exclusion for the second parent (CPE2 = 99.98%) met the minimum standard of international human paternity test. Among all 18 identified birdlings and helpers, bi-parents of eight offspring were successfully i...
Guofang Wu1,2, Xingxing Xue1, Wenjuan Shen1, Lei Wang1,2*, Yuhong Ma1 and Jiping Zhou1
... genetic marker, such as microsatellites. Based on our preliminary sequencing data, the present study describes the use of time-of-flight mass spectrometry (TOF-MS) to identify the locations of four new microsatellites in Qinghai Bamei pig (n=256), an indigenous breed of Chinese pig. TOF-MS demonstrated that the L1-(GT)n locus had three alleles: 152 bp, 164 bp, and 166 bp. The three other loci, L2-(AC)n...
J.Y. Bai1,*, R.T.D. Wu2,*, Q. Zhang2, D. Bao2, L.M. Dao2 and X.H. Tian3
...mall;">In this study, 17 microsatellite markers were used to analyze the genetic polymorphism, genetic differentiation, gene flow and genetic distance of Alashan desert Bactrian camel, Alashan Gobi Bactrian camel, Sunite Bactrian camel and Qinghai Bactrian camel.The results indicated that the number of effective alleles of the four Bactrian camel varieties ranged from 2.7302 to 3.0524, and average theoretical heterozygosity and average polymorphic information ...
Hui-Ming Li1, Bi-Ze Yang1, Xue-Jun Jin1,2, Lin-Miao Li1, Hai-Ying Jiang1,
Xiu-Juan Zhang1 and Jin-Ping Chen1*
...dy demonstrated that the microsatellite-based approach could be effectively utilized for parentage analysis in pangolins and that it has a significant application in selective breeding in endangered and valuable mammalian species.
...
Junyan Bai1*, Rentaodi Wu2*, Qihang Hu1, Lema Dao2 and Xinghua Tian3
... the correlation between microsatellite marker polymorphism and wool fineness of sunite bactrian camel was analyzed. The results show that, the average effective allele number, heterozygosity and polymorphism information content of 17 microsatellite markers in sunite bactrian camel were 2.8913, 0.6205 and 0.5602, respectively. The wool fineness of AB genotype of CMS36 locus was significantly higher than that of BB genotype (...
He Yujiao1,2, Xie Huichun1, Lin Gonghua2,3, ZhangTongzuo2,3, Su Jianping2,3 and Du Yurong1*
...rated using mtDNA versus microsatellite markers. The most obvious difference between the two markers was reflected in the fact that the populations Yelashan (YLS) and Bangda (BD) represented a separate cluster based on the microsatellite data. Additionally, our studies involving the comparison of the two markers also reveal that a few of the differentiated populations from the middle region have a higher admixture. Due to a ...
Mary A. Sargious1, Hanaa A. Ahmed1, Ragab M. El-Shawarby2, Hatem H. Bakery2, Sherif I. Ramadan3, E. Gus Cothran4 and Ayman Samir Farid5*
...ood were genotyped by 16 microsatellite markers. The purposes of this study were; firstly, to investigate the current status of the genetic diversity and inbreeding of Arabian horse populations reared in Egypt. Secondly, to examine the traditional maternal based strain classification system “Al Khamsa” using samples of native Arabian horses reared in the El-Zahraa stud based on 16 microsatellite markers. El-Zahra...
I Nyoman Sulabda1*, I Nengah Wandia2, I Ketut Puja3
...s in BoLA gene, the BoLA microsatellite loci (DRB3 and BM1815) were used to evaluate allel diversity using 18 samples of Taro white cattle. Genetic variability was low, with observed heterozygosity and expected heterozygosity for locus BM1815 was 0.778 and 0.51, and for DRB3 was 0.556 and 0.478, respectively. Moreover, the polymorphic information content (PIC) for these two microsatellite was 0.397 for BM1815 and 0.413 for ...
Maskur Maskur1*, Muhammad Muhsinin1, Lestari, Tapaul Rozi1, Asnawi2
...hometric traits and four microsatellites marker. Experimental ducks consisted of 75 Sasak ducks, 75 Khaki Campbell ducks and 70 Mojosari ducks. Morphometric traits were recorded and statistically analyzed, whereas genetic parameters of microsatellite loci were calculated using PopGene32. In this study, the mean PIC of the 4 microsatellite loci in all populations was 0.472, whereas AY287 wa...
Mudassar Jehan1, Masroor Ahmed Bajwa2, Mohammad Masood Tariq2, Asim Faraz3*, Abdul Samaad2, Jameel Ahmad1 and Yousaf Hassan Barozai2
....2166 to 0.95182 for the microsatellite markers employed in the study. Expected reduction in heterozygosity was higher than mean value in case of most of the markers with lower standard errors showing the prevalence of homozugous Balochi sheep population. The main cause reason behind prevalence of homozygous population might be inbreeding as only few rams had been used for breeding the flocks. This study would provide basis for breed characterization and lead ...
Yue Ren1, Ting Jia2, Hao Zhang1, Zhengkun Wang1 and Wanlong Zhu1*
...ianma and Lijiang. Using microsatellite approaches, we observed considerable genetic variation and high heterozygosity in T. belangeri chinensis populations in different Yunnan province, and Chuxiong population and Lijiang population had relative low genetic diversity. Moreover, skull morphology changed for adapting to changing environment in T. belangeri chinensis. Finally, MengLa population had remarkable differences with the other populations both in geneti...
Xiao Zhang1, Guoqing Shen1, Yang Wang1, Pengdan Huang1,2, Yan Shui2, Zenghong Xu2 and Huaishun Shen1, 2*
...the genome. However, the microsatellite markers available for the red swamp crayfish Procambarus clarkii are limited. In this study, a sizeable number of microsatellites were identified using the data of the P. clarkii genome survey generated from Illumina Hi-Seq PE sequencing. A total length of 1,625 Mb genomic sequences of P. clarkii were assembled based on 124 Gb raw sequencing data. Based on a Kmer analysis, the P. clark...
Shafia Riaz1, Khalid Abbas1*, Tanveer Ahmed1,2*, Huma Naz3, Hina Amjad1 and Shahbaz Ahmad1
...ies specific polymorphic microsatellite markers. Overall, the results of growth trial confirmed that FQB genotype performed maximum while QBD evinced lowest performance among all the genotypes based on wet weight (g) and total length (mm). Genetic analysis revealed significant differences based on allelic frequencies, heterozygosity, inbreeding and genetic distance among hatchery genotypes. Impact of G×E interaction is illustrated by results where geneti...
Safriyanto Dako1, Nibras Karnain Laya1, Syukri I Gubali1, Ari Ardiantoro2, V.M Ani Nurgiartiningsih3, Gatot Ciptadi3, Desinta Wulandari3, Suyadi Suyadi3*
... and relationships using microsatellite markers between the populations of Gorontalo cattle in Gorontalo Province, Indonesia, using 3 microsatellite markers. A total of 126 samples of local bovine blood were collected, which consisted of North Gorontalo (n=28), Gorontalo (n=52), Bonebolango (n=37), PO (n=3), and Bali (n=6), were used in this study. The Genomic DNA Mini Kits were used for DNA extraction for an analysis fragme...
Ruobing Han, Shanshan Gui, Xunwu Zhao and Heping Li*
... population. We used ten microsatellite loci to genotype all 28 white-red deer individuals in China, and constructed a Neighbor-joining (NJ) tree based on genetic distances. In addition, genetic structure analysis was carried out. The results showed that a total of 67 alleles were detected, and the average number of alleles at each locus was 6.7. There was high genetic diversity but severe inbreeding in the white-red deer population, and there is no father-mot...
Yanping Li, Fei Xu, Yongming Wang*, Yunyun Lv, Jinrong Shi, Biwen Xie, Wenyao Cai and Dian Liu
...evesae ♀. Secondly, 19 microsatellite loci were used to evaluate genetic diversity between hybrid offspring and their parents. The mean polymorphic information content (PIC) among groups ranged from 0.685 to 0.818, implying high polymorphism in all of the four groups (PIC > 0.5). The mean observed heterozygosity ranged from 0.779 to 0.887, and the hybrid combination of S. superciliaris ♀ × S. reevesae ♂ obtained the highest observed heterozygosi...
Qi Liu1,2, Weiyuan Li2,3, Qi Zhang2,3, Ziwei Wang2,3, Rui Gao1,2, Wenlei Liu1,2, Lei Zhang1,2, Ying Liu1,2, Tao Tian2,3 and Hongwei Yan2,3*
... five years. A set of 13 microsatellite markers were used to evaluate genetic patterns across 2015, 2016 and 2019. We observed a significant Hardy-Weinberg disequilibrium across all stocks. The inbreeding coefficient (Fis) was positive (0.121–0.131) for all stocks. A loss of genetic diversity was detected in the 2019 stock and significant differences were observed for number of different alleles, number of effective alleles, allelic richness and unbiased...
Yingying Ye1, Chengrui Yan1, Kaida Xu2, Jiji Li1, Jing Miao1, Zhenming Lü1 and Baoying Guo1*
...veloped primers for nine microsatellite loci resulting from an enrichment process based on the Wenzhou population. Eight out of nine microsatellite loci were used to analyze population genetic diversity of cuttlefish in China Sea. The results showed high levels of genetic diversity in all populations with highest mean number of alleles and mean effective alleles estimated in population Ningde and lowest in Qingdao. Heterozyg...
Mamona Jabeen1, Sumra Naz1, Hina Amjad1, Khalid Abbas1*, Sajid Abdullah1, Muhammad Anjum Zia2, Taqwa Safdar1 and Muhammad Sarfraz Ahmed1
...as studied by using five microsatellite loci in a total of 150 individuals. The C. marulius population exhibited a moderate level of heterozygosity. The mean value of observed heterozygosity ranged between 0.700 – 0.833 while the expected heterozygosity varied between 0.863 – 0.868. Significant deviation from HWE (P<0.05) was observed in all the populations due to the deficits of heterozygotes suggesting either due to inbreeding or recent mixing...
Taqwa Safdar, Khalid Abbas*, Muhammad Sarfraz Ahmed, Hina Amjad, Sumra Naz and Jamal Kazam
...er was assessed by using microsatellite markers. For the purpose, samples were collected from five selective Indus River sites, and DNA extraction was done by proteinase-K and standard phenol/chloroform DNA isolation method. Genomic DNA was PCR amplified by Labeo rohita cross-species amplification of H. molitrix. The results showed a low-to-moderate level of genetic diversity. The number of alleles on each locus ranging from 2.0 to 6.0, with an average of 3.48...
Haifen Qin1,2, Yujia Liu1, Yujie Zhang1, Jingfeng Liu1, Zhenkun Zhao1,2 and Lichun Jiang1,2*
...-A stretch region, three microsatellite-like repeats of (TA)n and three other random repetitive sequences. Based on 13 PCGs of 118 Cucujiformia mitogenomes, the phylogenetic analyses are reconstructed with both maximum likelihood and Bayesian analyses and a consistent topology is formed. The results show that A. horsfieldi grouped with A. glabripennis and A. chinensis with high nodal supports. It also supports such phylogenetic relationships of ((Lymexyloidea ...
Van Dai Nguyen1, ¥, Van Ba Nguyen2, ¥, Thi Thanh Nhan Giang2, Cong Dinh Nguyen2, Thi Lan Nguyen1, Dinh Ngoan Vu1, Duc Chuyen Nguyen1, Van Can Ta1, Khac Khanh Nguyen2, Thi Lien Cao2, Doan Lan Pham2, Khanh Van Nguyen2, Ngoc Tan Nguyen3*
...nd 32 TC and a set of 18 microsatellite markers was used to analyze their genetic diversity. Results showed that all the microsatellite markers were polymorphic in the two local swamp buffalo populations. The average number of alleles of each microsatellite marker in the two native buffalo populations varied from 6.11 (in LB-LD and LB-LC sub-populations) to 7.67 (in the TC population), wit...
