Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
...fic molecular markers in genotyping and genetic identification is of essential significance for preserving genetic diversity. The aim of this study was to reveal the genotype profile of indigenous types for TGC breed. In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatin gene CAST were studied. In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enz...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...tani hospitals. Ala54Thr genotyping of 200 MetS patients and 200 healthy controls was performed using a PCR-RFLP. Genotype and allele frequencies were calculated and computed for genetic associations presented as odds ratios (ORs) with a threshold of p≤0.05 to determine statistical significance. The frequency of 54Thr allele of FABP2 significantly associated with MetS risk in Pakistani population. Our results showed that frequency of the FA...
Shagufta Naz*, Saima Sharif, Hafsa Badar, Syeda Fareeha Tauheed
...uation, early diagnosis, genotyping, genetic counseling and proper treatment (corneal grafting) are necessary for the restoration of optimal vision and should be done to avoid the severe outcomes caused by MCD.
... 
Ali Mahmoud Zanaty, Naglaa Mohammed Hagag, Neveen Rabie, Mahmoud Saied, Karim Selim, Saad A. Mousa, Azhar Gaber Shalaby, Abdel-Sattar Arafa and Mohamed Khalifa Hassan
...ive NDV by rRT-PCR using genotyping primers, with a prevalence rate of 16.2%. NDV outbreaks were recorded in 16 governorates, from total of18 investigated governorates and the recorded geo-prevalence of 89 %. Twenty-five samples were selected for further sequencing for the partial fusion protein. Phylogenetic analysis revealed that 20 samples are genotyped as very virulent NDVclass II of genotype VIIb, 4 samples were of high identity (94%-100%) with NDV class ...
Hafiz Suboor ul Hassan and Saira Malik*
...er ARMS-PCR was used for genotyping. 79.48% lactose intolerant individuals showed homozygous LCT-13910CC genotype and 16.66% showed heterozygous CT genotype. For LCT-22018G/A, 43.75% lactose intolerant individuals showed homozygous GG genotype, while 43.58% patients showed heterozygous GA genotype. Thus, different variants of genotype -13910T/C and -22018G/A of the individuals were found to be associated with the development of lactose intolerance in patients ...
Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2
...esigned to determine the genotyping of clinically isolated HCV from a Liver Center Faisalabad, Pakistan. A total of 3200 clinical samples were screened for the presence of anti-HCV antibodies by ICT method. Among positive samples, confirmation was carried out by RT-PCR and clustered into four groups (acute, chronic, cirrhotic and hepatocellular carcinoma) on the basis of clinical reports and the patient’s history. HCV genotypes were determined by RFLP us...
Asima Rani1,*, Syed Kashif Nawaz2 and Muhammad Arshad3
...polymorphism. Results of genotyping were confirmed via PCR-RFLP strategy. Presence of GG genotype decreases the susceptibility of malaria (OR: 0.544, CI: 0.331 to 0.894, p=0.053), mild malaria (OR: 0.472, CI: 0.274 to 0.815, p=0.024) and P. vivax infection (OR: 0.362, CI: 0.211 to 0.622, p=0.000). AG heterozygosity increased the susceptibility of malaria (OR: 1.835, CI: 1.117 to 3.013, p=0.053), mild malaria (OR: 2.115, CI: 1.226 to 3.649, p=0.024) and ...
Q. Sun1, Q. Liu1, R. Di1, Y. Wang1, S. Gan2, S. Liu2, X. Wang1, W. Hu1, X. Cao1, Zh. Pan1, X. Guo1, Y. Yang3, H.E. Rushdi4* and M. Chu1*
Saima Yaqub1, Tahir Yaqub1*, Muhammad Zubair Shabbir2, Asif Nadeem3, Aziz-Ul-Rahman1, Muhammad Furqan Shahid1, Zarfishan Tahir4 and Nadia Mukhtar4
...; 11.54%). Subsequent to genotyping resistance interpretation algorithm, one major (M46L) and two accessory (N88D, L89V) PIs mutations in the protease region while four NRTI (D67T, K70R/Q, M184V and T215F) and four NNRTI (V108T, E138A, V179I and Y181C) mutations in the reverse transcriptase region were observed. The present study concludes circulation of multiple subtypes of HIV-1 among IDUs and a continuous disease surveillance coupled with delineation of dis...
Saad A. Moussa1, Mohammed Ahmed Mahmoud Abdullah2*, Mahmoud Saied1, Mustafa Saleh1, Mohamed A. Soliman2 and Ali Mahmoud Zanaty2
Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.
Fawzy Rania1, AboElkhair M.1, Bazid A.M.1, Sultan H.2, Hussien A.H.3
...lution, epidemiology and genotyping of Egyptian IBV field strains is very important for screening the emergence of new variant strains as well as evaluating the existing vaccination programs....
Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1
Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2
Abdullah Sheikh1,*, Faisal Almathen1,2 and Hairul Islam Mohamed Ibrahim3,4
...n which is supportive in genotyping, genetic selection, breeding and classification.
...
Ewa Czerniawska-Piątkowska1, Iwona Szatkowska1, Daniel Zaborski2*, Wilhelm Grzesiak2, Sara Tabor-Osińska1, Małgorzata Wasielewska1, Witold S. Proskura1, Wojciech Kruszyński3 and Edward Pawlina3
...s. PCR-RFLP was used for genotyping. A bioinformatic analysis of the P1 regulatory sequence was also carried out. Three genotypes (TT, CT and CC) were identified. The CT genotype was the most frequent (0.40, 0.50 and 0.52 in Jerseys, HO and RW, respectively). The frequencies of the CC genotype were 0.22, 0.28 and 0.31 in the RW, Jersey and HO breeds, respectively, whereas those of the TT genotype were 0.19, 0.26, and 0...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*
...aureus, we conducted genotyping of Staphylococcus aureus isolates from milk samples collected from cows with clinical mastitis using the random amplified polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5 different provinces in China, including Guizhou, Inner Mongolia, Shanghai, Sichuan, and Gansu. The results showed that the amplified product band...
Iqra Mobeen1*, Rabia Arif1*,Maimoona Ilyas1, Siu Fai Lee2 and Muhammad Saleem1
M. Hanafy1, Rehab Elhelw2, Soliman M. Soliman3, Sherif Marouf2*
Guangming Xiang1, Ran Di1, Yaowu Wang1, Shangquan Gan2, Shouren Liu2, Xiangyu Wang1, Wenping Hu1, Qiuyue Liu1* and Mingxing Chu1*
Hadia Gul1, Abdul Haleem Shah1, Ricardo Harripaul2, Anna Mikhailov2, Ejaz Ullah Khan3, Wasim Shah3, Nisar Ahmad3, John B Vincent2,4 and Muzammil Ahmad Khan3*
...ormed through microarray genotyping and homozygosity-by-descent (HBD) mapping, whole exome sequencing (for mutation identification) and Sanger sequencing (for variant segregation). Homozygosity analysis revealed a 1.2 Mb HBD region on chromosome 15 between markers rs4778147 to rs8036234 (chr15:27752745-28962131bp), which harbors the previously reported OCA2 gene. The subsequent whole exome sequencing identified a novel splice site defect at spice donor site, w...
Hua Liu, Wei Hou, Li Peng, Changjun Peng, Yansen Cai and Jing Li*
...een laboratories and low genotyping error, single nucleotide polymorphism (SNPs) are considered to be preferable molecular markers for genetic population analysis. SNP markers that are suitable for non-invasive samples are important to wild population investigations in endangered species. Based on the whole genome sequences of Macaca thibetana, we successfully developed 26 SNP loci that were sensitive to non-invasive samples, then based on which, genetic diver...
Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4
...ds | VP2 gene, Molecular genotyping, Phylogenetic analysis, Viral evolution. ...
Nour H. Abdel-Hamid1*, Walid Elmonir2, Eman I. M. Beleta1, Rania I. Ismail1, Momtaz Shahein1, Mahmoud E. R. Hamdy1
...morphism DNA (RAPD), for genotyping the Brucella isolates, as alternative rapid tools for epidemiological tracing and investigation of brucellosis in Egypt. We recovered Brucella isolates (n=29) from different host species and governorates. The isolates were identified by the bacteriological and molecular techniques (AMOS-PCR) as Brucella (B) melitensis biovar 3 (n=24) and B. abortus bv1 (n=5). The ERIC and RAPD primers (ERIC2 and Operon 18/ RAPD4) used i...
Ali Basim Abd Al-Hadi*, Tahreer Mohammed Al-Thuwaini, Mohammed Baqur Sahib Al-Shuhaib
...ncy and 109 with twins); genotyping and sequencing were used to confirm the variants in the amplified fragment of the FST gene (exon 4). Results of the genotyping technique identified three genotypes: CC, CG, and GG. Sequencing analysis revealed a novel mutation c. 25760691 C>G in the CG genotype. Association analysis revealed significant differences (P ≤ 0.05) between CC and other genotypes in reproductive hormone lev...
Barbosa Carla1*, Gregori Fabio2, Thomazelli Luciano1, Oliveira Amanda1, Araújo Jansen1, Ometto Tatiana1, Marcatti Roberta3, Nardi Marcelo3, Paludo Danielle4, Utecht Nathalia1 and Edison Durigon1
...of CoVs confirming their genotyping to Gammacoronavirus genus and proximity to important poultry diseases causative agents....
Doungnapa Promket1,2,4*, Khanitta Pengmeesri2,4, Jennarong Kammongkun3, Thassawan Somchan2
...d from blood samples for genotyping using specific primers and restriction enzymes for each gene, and polymerase chain reaction-restriction fragment length polymorphism was used to identify the genotypes (PCR-RFLP). Three genotypes were found for each gene as BB, Bb and bb for NPY; TT, TC and CC for DRD2 and II, ID and DD for VIP. Genotype frequencies of NPY (range 0.13-0.58), DRD2 (range 0.06-0.55) and VIP (range 0.14-0.57) were reported. For the NPY gene, al...
Umara Amir ud Din1, Waqas Ahmed Khan1*, Khurrum Shahzad1,2, Basharat Ali3, Misbah Hussain1,4 and Fazli Rabbi Awan4
Mai M. Dawood*1, Mohamed Fawzy2 and Mohamed S. Elshahidy3
...ulent IBDV (vvIBDV). The genotyping of Egyptian vvIBDv indicate progressive evolution compared with previously isolated strains which indicates persistence of vvIBDv in Egyptian poultry environment. In conclusion, theses data reveal the success and nonstop evolution of the vvIBDv in the Egyptian environment. This is why we must do further analysis in result of the nature of the viruses.
...
Rimsha Ijaz, Rida Zahra, Naseer Ali Shah*, Haroon Ahmed, Nazish Bostan and Sadia Sattar
...pe is identified through genotyping method. In the HCV positive sample, genotype 3a was the most common genotype, accounting for 652 (76.3%) of the infected people. Given the enormous morbidity and mortality caused by HCV, having precise detection methods that allow for early diagnosis and care for better outcomes is critical....
Muhammad Haseeb Malik2, Muhammad Moaeen-ud-Din1*, Ghazal Kaukab Raja2 and Feroza Hamid Wattoo2
...kers for high throughput genotyping. The allele frequencies in both breeds were determined for discrimination ability using AFLP. The probability of identifying Sahiwal breed was 0.86 and probability of misjudgment was 0.021 using single selected marker. However, probabilities for judgment and misjudgment with two markers and combined with three markers were 0.745, 0.367 and 0.964, 0.376 respectively. The results demonstrated that Sahiwal breed and crossbred c...
Skorykh Larisa Nikolayevna1*, Fominova Irina Olegovna1, Kovalenko Dmitriy Vadimovich1, Skokova Antonina Vladimirovna1, Dmitrik Irina Ivanovna1, Kizilova Natalia Igorevna2 and Violeta Caro Petrovic3
M.R. Abd-El Wahab l, H.A. Hussein2, T.M. Asfourl and M.A. Shalaby2
...sitive results. However. genotyping of such positive samples using RT-PCR genotyping based assay revealed negative for the presence of BVDV type I, II, and border disease virus. After 3 passages of the detected positive buffy coats on MDBK cells. no CPE was detected suggesting that these viruses may be of non-cytopathic type of BVDV. The present study confirms the existence of BVDV among camel population and raises the possi...
Doungnapa Promket1*, Khanitta Pengmeesri1, Jennarong Kammongkun2, Thassawan Somchan1
... study employed PCR-RFLP genotyping of the MTNR1C and NPY genes to investigate their association with egg production traits. The results revealed significant associations between these genes and several egg production parameters on Thai indigenous chicken populations. In the case of the MTNR1C gene, significant effects were observed on WE360d, NE360d, and E/M (P < 0.05). Specifically, Thai indigenous chickens carrying the AA genotype in the MTNR1C gene disp...
Zeynep Karapinar1* and Mehmet Özkan Timurkan2
MUHAMMAD NAEEM RAZA1, KALSOOM SUGHRA1* & NADIA ZEESHAN1
...e subjected to molecular genotyping on Real Time PCR (Applied Biosystems, Step One software). Results show 2750 out of 3540 (77.6%) suspected patients were positive for HCV-RNA. Female have higher percentage (57.78%) than male (42.22%) with highest viral load observed at age group 15 to 30 years. The genotype 3a (83.33%) was dominant to other circulating genotypes as 2b (13.33%) and mixed 3a/2b (3.33%). The highest prevalence of HCV infection was noted in fema...
Ngoc Tan Nguyen1*, Thi Tuong Vi Trang1, Thi Thuy Tien Nguyen2, Tuan Thanh Hoang2, Duc Thoa Nguyen2
...is on 2% agarose gel for genotyping. Polymorphism was observed at the 24 bp PRL/indel locus, showing two alleles I and D with frequencies 0.57 and 0.43, respectively and three genotypes (II, ID, and DD) with frequencies 18.3, 49.4, and 32.3%, respectively. Hens with the DD genotype had a significantly (P<0.05) higher ANE than hens with the ID or II genotypes. The regression equation, Y = 1.5437X + 58.386 (R2=0.0185) showed a positive correlation with the co...
Zuhair Dardona1,2, Adnan Al-Hindi3, Mohamed Hafidi1, Ali Boumezzough1 and Samia Boussaa1,4*
... detected using PCR. The genotyping results of the current investigation showed that the three DNA isolates were T. gondii type II. A Chi-square test was also implemented to evaluate the prevalence of T. gondii and the type of red meat samples (mutton and beef) examined using PCR and ELISA. Similarly, in the detection of T. gondii, a comparison of the PCR approach and ELISA was conducted, and all of these relationships were shown to be statistically significan...
