Issue · 2024
Pakistan J. Zool., Vol. 56
85 articles · All issues →
Research ArticleExploring Helicobacter pylori’s Impact on Clinical Manifestations and Gastroesophageal Reflux Disease Spectrum
Syed Rafiq Hussain Shah, Atta Ullah, Dhafer Hazip Gaber Alwayli, Jiaxu Liang, Tayyab Saeed Akhter, Faisal Rasheed et al.
Gastroesophageal reflux disease (GERD), a disorder resulting from the retrograde flow of gastric contents into the esophagus, affects an estimated 10–30% population worldwide. Several pathophysiological factors influence the development and the course of…
21 Mar 2025DOI 10.17582/journal.pjz/20230603100611
Research ArticleEffects of Insulin Intensification on FINS, CP, HOMA-IR, and HbA1c Levels in Patients with Gestational Diabetes Mellitus
Qian Liu, Minhuan Sun, Zijuan Xu
The objective of this study was to evaluate the effects of insulin combined with metformin on glycemic control and fasting insulin (FINS), C-peptide (CP), homeostasis model assessment of insulin resistance (HOMA-IR) and glycated hemoglobin (HbA1c) levels in pa…
20 Mar 2025DOI 10.17582/journal.pjz/20231210184756
Research ArticleAn Experimental Study of Acupuncture to Rat Model of Obesity: Involvement of SIRT1 Signaling Pathway
Muzhi Pan, Pengli Si, Ping Zheng, Bingwei Ai
Obesity is a global health challenge. While manual acupuncture has been recognized for its potential to reduce body weight, the efficacy of different manual acupuncture techniques in obesity treatment remains to be elucidated. This study aims to investigate th…
22 Mar 2025DOI 10.17582/journal.pjz/20231122060150
Research ArticleGenotoxicity and Anti-Cancer Activity of Tamoxifen and Ivermectin Loaded Chitosan Nanoparticles Against MCF-7 Cell Line
Usama Bin Naeem, Muhammad Adil Rasheed, Muhammad Ashraf, Muhammad Yasir Zahoor
The purpose of this work was to examine the anti-proliferative activity of tamoxifen (TAM) and ivermectin (IVM) loaded chitosan (Cs) nanoparticles against breast cancer cell line (MCF-7) to improve drug delivery to the target sites. Characterization of NPs wer…
21 Mar 2025DOI 10.17582/journal.pjz/20230207150234
Research ArticleMolecular Metabolism in Treatment of High Altitude Polycythemia with Duoxuekang Capsule
Jia Nie, Ze Weng Yong Zhong, Di Wang, Ming Jian Li
The objective of this study was to explore the molecular metabolic mechanism of Duoxuekang capsule curing high altitude polycythemia (HAPC) from two aspects of efficacy and mechanism. Sixty SPF grade male SD rats aged 1 month were purchased from the experiment…
22 Mar 2025DOI 10.17582/journal.pjz/20240128104955
Research ArticleIdentification of Therapeutic Targets and Prognostic Biomarkers Among Matrix Metalloproteinases in the Ovarian Cancer Microenvironment
Mao MeiYa, Sheng Yuehua, Ding Huiqing, Zheng Xiaojiao, Du Yongming
Ovarian cancer (OC) is one of the most common malignances with an ever-increasing incidence and high mortality. Cross-talk between cancer cells and interstitial cells exerts significant effects on ovarian and tumor development and is modulated in part by MMPs.…
20 Mar 2025DOI 10.17582/journal.pjz/20231205030220- Short Communication
Effect of Low-Molecular-Weight Heparin Calcium on Senile Diabetes Nephropathy
Haiyan Li, Dan Li, Yashi Xu, Xingxiang Guo
The objective of this study was to explore the impact of calcium-based low-molecular-weight heparin on nephropathy in elderly individuals with diabetes. We selected ninety elderly patients diagnosed and treated at our hospital from March 2020 to October 2022.…
23 Mar 2025DOI 10.17582/journal.pjz/20231005193609 
Short CommunicationA Comparative Analysis of Baseline Variables in Responder and Non-Responder Groups of Chronic Hepatitis C patients
Muhammad Shahid, Iram Amin, Rabia Nawaz, Samia Afzal, Afza Rasul, Muhammad Umer Khan et al.
The chronic hepatitis C (CHC) virus plays a vital role around the globe in hepatic persistent disease. It is undoubtedly a major health and financial burden in Pakistan, affecting about 6 to 10 percent of the overall population. The purpose of this research is…
23 Mar 2025DOI 10.17582/journal.pjz/20230119060159- Research Article
Effects of Shenling Baizhu Powder on the Proliferation and Invasion of Tumor Cells: Regulating Polarization of Macrophages in Co-Culture System
Wei Tian, Junquan Han, Wenheng Han, Jia Wei, Hong Wang
The objective of this study was to explore the effects of Shenling Baizhu powder on the proliferation and invasion of tumor cells by regulating macrophage polarization in the co-culture system. Raw264.7 macrophages were added with IL-424h to lead M2-like macro…
20 Mar 2025DOI 10.17582/journal.pjz/20231227111732 
Research ArticleAlpha-2-Macroglobulin and Alpha-2-HS Glycoprotein are Potential Markers of Renal Cell Carcinoma: An Insight from Proteome Profile of Cancer Tissues
Safa Akhtar, Shahzadi Noreen, Anna E. Lokshin, Muhammad Waheed Akhtar
Renal cell carcinoma (RCC) is characterized as the most common neoplasm of the human kidney among the top fifteen most diagnosed tumors, encompassing multiple subhistologies with definite genomic, clinicopathological, genomic and proteomic features. Proteomics…
4 Mar 2025DOI 10.17582/journal.pjz/20220923120947- Research Article
Effect of Moringa oleifera Leaf Meal on Mineral Contents of Labeo rohita Fingerlings
Muhammad Zubair-ul-Hassan Arsalan, Syed Makhdoom Hussain, Basharat Ali, Bilal Ahmad, Muhammad Qurban, Adan Naeem et al.
Fish meal, a highly digestible source of protein in aqua feed, has not been readily available to fulfill the need for sustainable aquaculture production due to its limited supply and high price. So, the aim of this study was to develop more sustainable and eco…
9 Mar 2025DOI 10.17582/journal.pjz/20230413200442 - Short Communication
Haematological and Biochemical Indices of Neonatal Intrahepatic Cholestasis Caused by Alagille Syndrome
Liu Nie
The aim of this study was to determine the blood routine and biochemical indices of Alagille syndrome (ALGS) and to explore the changes of the indicators of neonatal intrahepatic cholestasis caused by ALGS. This study was conducted on data of thirty-two neonat…
25 Mar 2025DOI 10.17582/journal.pjz/20240114052214 - Research Article
Protective Effect of Shengdi-Cogongrass Decoction on Patients with Blood Heat Psoriasis
Duo Yuan, Bin Shi, Yanhua Tu
Shengdi-cogongrass decoction (SCD) is a traditional Chinese medicine formula, mainly composed of raw Rehmannia glutinosa (RG) and lalang grass rhizome (LGR). However, little is known about the molecular pharmacological activity of Shengdi-cogongrass on blood h…
20 Mar 2025DOI 10.17582/journal.pjz/20231215095814 - Research Article
Effect of Platelet-Rich Fibrin Combined with Guided Bone Regeneration on Bone Formation of Peri-Implant Bone Defects
Li Yang, Xuliang Ma, Chao Wang
Platelet-rich fibrin (PRF) combined with guided bone regeneration (GBR) is an emerging treatment method. The purpose of this study was to demonstrate the effects of PRF on the osteogenic ability of peri-implant bone defects. The sample of 67 patients who neede…
4 Mar 2025DOI 10.17582/journal.pjz/20230526070557 - Research Article
Distribution of Pathogenic Bacteria of Postoperative Wound Infection and Early Prediction Value of Serum in Children with Hypospadias
Mimi Jin, Xiaoye Hu, Lijing Yu, Shuangli Zheng, Huishuang Huang, Fang Chen
The objective of this study was to investigate the distribution of pathogens and the early predictive value of serum in children with hypospadias. The sample consisted children with hypospadias between January 2019 and December 2021 at the Second Affiliated Ho…
9 Mar 2025DOI 10.17582/journal.pjz/20230602210624 
Research ArticleGinsenoside Rh2 Reduces Inflammation of Ulcerative Colitis via Elevating microRNA-30a-5p
Suanyuan Zhang, Qing Ji, Xinglong Wu, Jinjing Wang, Jin Yao, Chengfang Li et al.
Ulcerative colitis (UC) is a kind of chronic inflammatory bowel disease, closely related to colon cancer. Ginsenoside Rh2 (GRh2) is a rare protopanaxadiol type triterpene saponins in ginseng, which has anti-inflammatory/cancer/diabetic impacts. To investigate…
19 Mar 2025DOI 10.17582/journal.pjz/20220217080248
Short CommunicationImmunoregulatory Effect of Toll Like Receptor 9 Signaling Pathway in Neonatal Acute Lung Injury
Jun Mei
The purpose of this study was to investigate the impact of toll like receptor 9 (TLR9) signaling pathway and its immunoregulatory roles in neonatal acute lung injury (NALI) , laying a theoretical basis for the clinical therapy of NALI. Forty peripheral blood m…
25 Mar 2025DOI 10.17582/journal.pjz/20240121195923- Short Communication
Clinical Evaluation of miR-155 and miR-21 Alone and in Combination for Diagnosis of Acute Progressive Cerebral Infarction
Linyu Zhou, Hong Yu, Yang Wang, Jianbing Bai, Chuanyan Bao, Yongqing Dai
The objective of this study was to clinical evaluate miR-155 and miR-21 alone and in combination for the diagnosis of acute progressive cerebral infarction (APCI). One hundred twenty-nine patients with acute cerebral infarction (ACI) were collected as observat…
23 Mar 2025DOI 10.17582/journal.pjz/20231211144026 
Research ArticleEffect of Pyrotinib on Levels of Tumor Markers in Patients with HER2-Positive Breast Cancer
Biao Zhong, MengMeng Zou, Jie Zeng, Juan Bai, YongJun Deng, Xin Li et al.
Exploring effective treatment regimens for patients with HER2-positive breast cancer (HPBC) is of significant importance. Pyrotinib is a small-molecule tyrosine kinase inhibitor (TKI) developed in China and irreversibly binds to human epidermal growth factor r…
22 Mar 2025DOI 10.17582/journal.pjz/20240204075341
Research ArticleExploring Potential Novel Causal Genes for Chronic Kidney Disease
Fangyi Hao, Tian Tian, Zhen Yang, Jiaxin Zheng
Functional genes underlying chronic kidney disease (CKD) remain unclear. This work explored susceptibility genes related to serum creatinine (Scr) and CKD through Mendelian randomization (MR) analysis.Data from genome-wide association study (GWAS) were selecte…
21 Mar 2025DOI 10.17582/journal.pjz/20230817064609- Research Article
Effect of Clopidogrel Combined with Atorvastatin on Serum Lipids, Inflammatory Factors and Cardiac Function Parameters in Patients with Angina Pectoris and Coronary Heart Disease
Jiaojiao Ma, Jialei Jiang, Lijun Shan
The objective of this study was to analyze the regulatory effect of Clopidogrel combined with Atorvastatin on the low-density lipoprotein cholesterol (LDL-C), triacylglycerol (TG), and total cholesterol (TC) in patients with angina pectoris and coronary heart…
19 Mar 2025DOI 10.17582/journal.pjz/20230816081639 
Research ArticleExpression of Aquaporin-1 and Endothelin-1 in Children with Congenital Hydronephrosis
Jinjian Ma, Zhaokang Wang, Hong Liu
The objective of this study was to analyze the expression of aquaporin-1 (AQP-1) and endothelin-1 (ET-1) in children with congenital hydronephrosis. A study group consisted of 52 children who were admitted to our hospital from August 2021 to August 2022 and di…
19 Mar 2025DOI 10.17582/journal.pjz/20230730043006
Research ArticleAdenosine Deaminase Acting on RNA1 (ADAR1) Deficiency Promotes Proliferation of Hemangioma-Derived Endothelial Cells via PI3K/Akt/mTOR Pathway
Chaoqi Yin, Ruyi Tao, Kaixi Tan, Jianfei Zhang
Infantile hemangioma (IH) is one of the most common benign tumours occurs in infancy. The adenosine deaminase acting on RNA1 (ADAR1) activates phosphorylated Akt (p-Akt), but the roles of ADAR1 remain unknown in IH. The objective of this study was to investiga…
10 Mar 2025DOI 10.17582/journal.pjz/20230601050629
Research ArticlePreparation and Characterization of Iron Oxide Nanoparticles for Effective Epirubicin Delivery to Drug Resistant Breast Cancer Cells
Sayra Tariq, Zeeshan Mutahir, Moazzam Ali
Chemoresistance is toughest of all challenges in the fight against cancer. The incorporated drugs are pumped out of the cells due to a number of reasons. However, if these drugs are attached to nanoparticles, they are likly to be retained by the cells for long…
10 Mar 2025DOI 10.17582/journal.pjz/20230227180246
Research ArticleEffects of Desflurane on Inflammatory Response and Cardiac Function During Cardiopulmonary Bypass in Cardiac Surgery
Jingjing Guo, Ruitong Niu, Shanshan Mu, Anshi Wu
The objective of this study was to investigate the effect of desflurane on inflammatory response and cardiac function during cardiopulmonary bypass in cardiac surgery. Thirty adult male Sprague-Dawley rats (body weight, 250-300g) were purchased from the Provin…
10 Mar 2025DOI 10.17582/journal.pjz/20230813075411- Research Article
Effect of Calcium Supplement Combined with Carbetocin on Postpartum Hemorrhage in Scarred Uterine Cesarean Section
Guoqing Lai, Youxia Dai, Yuling Lan
Cesarean section (CS) in scarred uterus pregnancies (SUPs) is a high-risk obstetric procedure often associated with the risk of postpartum hemorrhage (PH). The objective of this study was to investigate the effect of calcium supplement combined with Carbetocin…
9 Mar 2025DOI 10.17582/journal.pjz/20230911194048 
Research ArticleEnhanced Antitumor and Anti-Metastasis Efficiency via Combined Treatment with Carvacrol and Doxorubicin
Hu-Hu Chen, Xin Li, Mohd Hijaz Mohd Sani, Siat Yee Fong
Recent research on treatment of triple-negative breast cancer (TNBC) has focused on drug combinations that can improve their anticancer properties while minimizing adverse effects. This study investigated the efficacy of combined treatment with doxorubicin (Do…
8 Mar 2025DOI 10.17582/journal.pjz/20211208031233
Research ArticleCPEB3 Targets E-Cadherin mRNAs in a Post-Transcriptional Regulation Manner and Inhibits the Invasiveness of Ovarian Cancer Cells
Yanhua Zhang, Hui Li, Xinya Li, Yong Zhang, Rongqing Li, Chen Qing
CPEB3 (cytoplasmic polyadenylation element binding protein 3) is a key factor that controls poly A tail extension during translation, exhibiting a tumor suppressor effect in several kinds of tumors. However, its expression characteristics and functional role i…
8 Mar 2025DOI 10.17582/journal.pjz/20210829050829
Research ArticleFactors Affecting the Absorption of Exosomes by Sertoli Cells
Ma Hong, Fu Bo, Wang Liang, Wang Fang, Guo Zhenhua, Liu Di
Exosomes play an important role in the signal transmission process of Sertoli (ST) cells. However, what factors affect the ability of ST cells to take up exosomes remains unclear. To investigate the influences of time, temperature, cytochalasin D (Cyt D), and…
3 Mar 2025DOI 10.17582/journal.pjz/20220727010714
Research ArticleEffect of Human Recombinant Activated Coagulation Factor VII (rFVIIa) on Total Bleeding Volume and Coagulation Function of Patients
Xiaolei Yan, Yabing Tang, Sainan Hu
The aim of this study was to analyze the effect of human recombinant activated coagulation factor VII (rFVIIa) on total bleeding volume and coagulation function of patients with postpartum hemorrhage (PPH). A total of 80 patients with PPH in Hunan Provincial M…
3 Mar 2025DOI 10.17582/journal.pjz/2023041107045- Research Article
Effects of Ginsenoside Rg3 on HLA-DR, HLA-ABC and Cellular Immune Function in Patients with Tumor Radiotherapy
Xiang Lv, Sufang Zhang, Li Ling, Xian Wang, Ying Xia, Xiaoping Wang et al.
The objective of this study was to study the effect of Ginsenoside Rg3 on HLA-DR, HLA-ABC and cellular immune function in patients with tumor radiotherapy. From July 2017 to August 2018, 80 patients who were diagnosed as tumor and received radiotherapy were ra…
3 Mar 2025DOI 10.17582/journal.pjz/20230428190417 - Research Article
Effect of Beauty and Freckle Removing Soup Combined with Reduced Glutathione on Oxidative Stress and Disease-Related Factors in Patients with Chloasma
Jing He
The objective of this study was to discuss the effect of Beauty and Freckle Removing Soup (BFRS) combined with reduced glutathione on oxidative stress (OS) and disease-related factors in patients with chloasma. Patients with chloasma were selected in West Anhu…
3 Mar 2025DOI 10.17582/journal.pjz/20230311050312 - Research Article
Analgesic Effect of Nalbuphine Combined with Sufentanil After Laparoscopic Hysterectomy
Zhenfeng Cai, Xingli Jiang, Xintao Dai, Pinglei Wu
The objective of this study was to examine the effect of PCIA analgesia after laparoscopic hysterectomy (LH) with Nalbupine combined with Sufentanil on Visual Analogue Scale/Score (VAS), Ramnsay sedation score, serum prostaglandin E2 (PGE2), 5-hydroxytryptamin…
1 Mar 2025DOI 10.17582/journal.pjz/20230331210308 
Research ArticleEvaluation of Serum PAF, 1,25-(OH)2D3 and MMP-9 Levels in the Diagnosis of Kawasaki Disease and Prediction of the Risk of Coronary Artery Lesion
Wei Li, Hui Xu, Si Li, Jing Liu
The objective of this study was to investigate the serum platelet activating factor (PAF), 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) and matrix metalloproteinase-9 (MMP-9) levels in the diagnosis of Kawasaki disease (KD) and prediction of coronary artery lesion…
1 Feb 2025DOI 10.17582/journal.pjz/20221224081215
Research ArticleRisk Factors Analysis of Hospital-Acquired Anemia in Cerebral Stroke Patients
Xinmin Liu, Fengming Ni, Lan Gao
We aimed to investigate the incidence and risk factors of hospital-acquired anemia (HAA) in stroke patients. We used retrospective analysis method, research on the incidence of HAA in 560 patients with cerebral apoplexy, the patients had incomed in the departm…
1 Feb 2025DOI 10.17582/journal.pjz/20220715060736
Research ArticleEfficacy of Amikacin Combined with Piperacillin/Tazobactam Sodium in Elderly with Severe Drug-Resistant Pseudomonas aeruginosa Pneumonia
Hongxiao Li, Xiying Wu, Dengxiang Wu, Jiangqing Yu
The purpose of this study was to demonstrate the effects of amikacin combined with piperacillin/tazobactam sodium (PPC/TS) in the therapy of severe resistant Pseudomonas aeruginosa (Pa) pneumonia in elderly individuals. The sample of 50 elderly patients with P…
31 Jan 2025DOI 10.17582/journal.pjz/20230524050529- Research Article
Association of TSLP Gene’s SNP Variants with Asthma Disease in Pakistan
Zohair Mehdi, Ifrah Khalid, Iqbal Bano, Muhammad Usman Ghani, Muhammad Farooq Sabar, Muhammad Umer Khan et al.
Asthma is the most common chronic inflammatory disease characterized by paroxysms of dyspnea, frequent wheezing, chest tightness, and cough that varies in intensity from person to person. Thymic stromal lymphopoietin (TSLP) is a cytokine residing within the cy…
4 Mar 2025DOI 10.17582/journal.pjz/20230218070210 - Research Article
Effect of Consistency of Diet on Swallowing and Blood Biochemical Components of Patients with Dysphagia After Brain Injury Surgery
Shuang Jin, Lishu Sun, He Wang, Yang Li
The objective of this study was to explore the effect of food consistency diet on different indicators and scales in patients with dysphagia after brain injury surgery. Patients (104) with traumatic brain injury who underwent surgical treatment were selected i…
1 Mar 2025DOI 10.17582/journal.pjz/20230315050318 
Research ArticleStudy on the Treatment of Experimental Dermatitis with Ginkgo biloba Leaf Extract and Quercetin
Minghui Xiao, Minjie Huang, Jie Dong, Deqian Wang
Allergic contact dermatitis (ACD), is a classic delayed allergic reaction mediated by cellular immunity. P815 degranulation model was established by stimulating P815 cells with C48/80 inducer to explore the effect and mechanism of quercetin on mast cell degran…
4 Mar 2025DOI 10.17582/journal.pjz/20220929090947
Research ArticleTCF3/ACSS2 Axis Promotes Proliferation and Colony-Forming Capacity of Esophageal Squamous Cell Carcinoma Cells
Hongqi Li, Meidong Xu
Esophageal carcinoma (ESCA), particularly esophageal squamous cell carcinoma (ESCC), is a prevalent and challenging health problem. Recent investigations have identified various transcription factors implicated in ESCC tumorigenesis and cancer progression, wit…
8 Mar 2025DOI 10.17582/journal.pjz/20220107070123
Research ArticleEffect of Hypertension on Blood Lithium Level and its Concentration to Dose Ratio in Manic Episode Patients
Dan Liao, Mingchao Li, Zijun Xiong, Qiuming Ji, Ying Xia, Bing Liu et al.
The objective of this study was to further explore the influence of hypertension on blood lithium levels and its concentration to dose ratio (C/D) in patients with manic episodes. A total of 112 patients with manic episodes from the Wuhan Wudong Hospital betwe…
8 Mar 2025DOI 10.17582/journal.pjz/20230728161028- Research Article
Association of Serum Inflammatory Factors and Hip Joint Scores with Adverse Prognosis After Internal Fixation in Patients with Femoral Neck Fractures
Hao Weng, Liang Zhu, Zhu Mei, Mingxian Wu, Qiang Xu
The objective of this study was to explore the relationship between adverse postoperative outcomes, changes in serum inflammatory factor levels, and hip joint functional scores in patients undergoing internal fixation for femoral neck fractures (FNF). A total…
10 Mar 2025DOI 10.17582/journal.pjz/20230908191620 - Research Article
Mendelian Randomization Analyses of Circulating Levels of Insulin-Like Growth Factor 1, Genetic Polymorphism, and Their Associations with Colorectal Cancer
Shuangmei Zhu, Xiang Lan, Hanying Wang, Jianan Hu
Insulin-like growth factor 1 (IGF-1) may exert a crucial role in the progression of colorectal cancer (CRC). This work aimed to investigate the Mendelian randomization (MR) relationship between circulating level (CL) of serum IGF-1, genetic polymorphism, and t…
10 Mar 2025DOI 10.17582/journal.pjz/20230930055647 - Research Article
Pathological Diagnostic Efficacy and Influencing Factors of Ovarian Tumour Using Cytological Smears Combined with Frozen Sections
Fang Li, Na Zhao, Dan Wu, Jingya Hei, Yuyang Pan
The objective of this study was to investigate the pathological diagnostic efficacy of applying cytological smears (CytS) combined with frozen sections (FroS) in patients with ovarian tumour (OT), and to study the relevant factors affecting the pathological di…
19 Mar 2025DOI 10.17582/journal.pjz/20230806091722 - Research Article
Expression and Promoter Methylation Status of CDX1 and CDX2 Genes in Chinese Patients with Gastric Cancer
Zhihong Huang, Xuemei Zhang, Ling Li, Min Hu, Mo Chen, Qunchao Mei
Gastric cancer is a multifactorial disease, the fourth most common cancer in the world and the second cause of cancer death. Changes in gene expression levels are one of the most important factors in the occurrence of cancer. Gene promoter methylation changes…
19 Mar 2025DOI 10.17582/journal.pjz/20231204081600 - Research Article
Molecular Characterization of Multi Drug Resistant Mycobacterium tuberculosis Isolated from the Patients in the Hospital of Azad Jammu and Kashmir
Sumayya Aziz, Abdul Rauf, Gotam Das, Muhammad Zahid Latif, Mohammed Merae Alshahrani, Nawal E Al-Hazmi et al.
Globally, Pakistan, with population of around 231.4 million, is at 5th spot in the list of countries with the highest number of TB patients in the world and it is further challenged by an increase in drug-resistant TB cases. The present study was conducted to…
22 Mar 2025DOI 10.17582/journal.pjz/20190324160313 - Short Communication
Correlation of Serum Pentraxin 3, Apelin and sRAGE with Obesity and Glycolipid Metabolism in Polycystic Ovary Syndrome
Yanxi Li, Yong Huang, Jun Peng, Yicun Man, Yaqi Li, Erqing Peng et al.
The objective of this study was to analyze the correlation of serum PTX3, apelin and sRAGE with obesity and glycolipid metabolism in polycystic ovary syndrome (PCOS). The sample of 82 PCOS patients was divided into the overweight subgroup and normal weight sub…
23 Mar 2025DOI 10.17582/journal.pjz/20230319170342 
Short CommunicationIdentification and Functional Analysis of miRNAs in PEDV-Infected PK-15 Cells
Miao Yin, Xi-Wen Chen, Zuo-Jie Xie, Cong Wang, Zhi-Chao Song, Jun-Qiao Chen
The aim of this experiment was to study the dynamic changes of PK-15 miRNA in porcine kidney passage cells infected by porcine epidemic diarrhea virus (PEDV). We collected samples from PK-15 cells at different time points after PEDV infection, analysed the cha…
23 Mar 2025DOI 10.17582/journal.pjz/20230526010532- Short Communication
An Analysis of Risk Factors for Asthenospermia: A Hospital-Based Study in China
Chengyuan Dong, Jianxin Sun
The objective of this study was to explore the influencing factors of asthenospermia. Electronic medical record and semen samples were collected from 201 male patients in The Fourth People’s Hospital of Taizhou from November 2021 to March 2023. Multivari…
23 Mar 2025DOI 10.17582/journal.pjz/20231111042600 - Short Communication
ω-3PUFA Inhibit Hepatic Fibrosis by Activating LKB1-AMPK Signal Pathway
Genfei Zhu, Xiaoqing Wu, Xianzhong Qian, Hong Hong
Hepatic stellate cell (HSC) lines were cultured in vitro and treated with different concentrations of Ω-3PUFA. The effects of Ω-3PUFA on the survival rate of HSCs were detected by MTT, and the α-SMA, collagen I, TIMP-1 and MMP-13 proteins wer…
24 Mar 2025DOI 10.17582/journal.pjz/20230804161622 
Research ArticleIdentification and Verification of Hub mRNA-miRNA-lncRNA Network on Psoriasis by Integrated Bioinformatics Analysis
Wen-Da Wang, Wei-Yue Gong, Bin Li, Shan-Shan Lei, Qing-Hua Wang
Psoriasis is an immune-mediated skin disease with a high incidence, it has been demonstrated that particular long non-coding RNAs (lncRNAs) are vital in psoriasis. However, their exact functions and regulatory mechanisms in psoriasis remain unclear. In this st…
4 Mar 2025DOI 10.17582/journal.pjz/20220310020325
Research ArticleEffect of TRAF6 on Proliferation of Prostate Transitional Zone and Peripheral Zone Cells
Yulin Zeng
The objective of this study was to demonstrate the effect of ubiquitin ligase tumor necrosis factor receptor-associated factor 6 (TRAF6) on promoting proliferation (Pro) of prostate transitional zone (TZ) and peripheral zone (PZ) cells by activating the insuli…
28 Feb 2025DOI 10.17582/journal.pjz/20230528150500
Research ArticleAlkB Homolog H5 Attenuates TGF-β1-Induced Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells via Regulating Zinc Finger E-box Binding Homeobox 1
Yunzhi Lin, Jing He, Xingwei Wu, Qian Shi
There is a relationship between proliferative vitreoretinopathy (PVR) and epithelial-mesenchymal transition (EMT) of Retinal pigment epithelium (RPE). The objective of this study was to investigate the role of AlkB homolog H5 (ALKBH5) in regulating EMT of RPE…
19 Mar 2025DOI 10.17582/journal.pjz/20231003105054
Research ArticleDynamic Changes of Immune Cells and their Relationship with Prognosis in Late-Stage Gastric Cancer Patients During PD-1 Inhibitor Immunotherapy
Chunqin Tian, Lichun Cui, Xiaoyan Wang
The study aimed to investigate the dynamic changes in immune cells during the programmed death-1 (PD-1) inhibitor therapy in patients with late-stage gastric cancer (GC) and to analyze the correlation between these changes and patient prognosis. One hundred th…
20 Mar 2025DOI 10.17582/journal.pjz/20240116074340
Research ArticleStudies on the Mitochondrial tRNA Gene Variants in Patients with Coronary Artery Disease and Pathogenicity Assessment by in silico Analysis
Fayaz Khan, Aziz Uddin, Hisham N. Altayb, Bibi Nazia Murtaza, Sajid Ul Ghafoor, Faisal Imam et al.
Mutations in mitochondrial DNA (mtDNA) have been associated with many pathological conditions including cardiovascular diseases. In the present study, we have evaluated the mutations in the mitochondrial tRNA genes for leucine, threonine and proline in patient…
22 Mar 2025DOI 10.17582/journal.pjz/20231226090544- Short Communication
Expression of Serum miRNA-137 in Women with Gestational Diabetes and its Relationship with Neonatal Hyperbilirubinemia
Meixiang Li, Meiyan Liu, Xuehua Sun, Juan Li
Diabetes is the most common endocrine disease in pregnancy, and identifying the risk factors of gestational diabetes mellitus (GDM) helps to discover women who are at risk of neonatal jaundice in the future. The purpose of this study was to investigate the exp…
24 Mar 2025DOI 10.17582/journal.pjz/20231012130923 
Research ArticleCarvacrol Induces Doxorubicin-Resistant Breast Cancer Cell Apoptosis via Inhibition of the PI3K/Akt Pathway
Hu-Hu Chen, Jiao-han Zhou, Siat Yee Fong
Doxorubicin (Dox), a widely used chemotherapeutic agent for breast cancer, is often ineffective due to the development of Dox resistance, posing a significant challenge in cancer treatment. Carvacrol is a monoterpene phenol with diverse biological activities,…
19 Mar 2025DOI 10.17582/journal.pjz/20220421100442
Research ArticleLemongrass Extract and Anticancer Impact: mRNA Levels of Expressing Apoptosis and Mitochondrial Fission in MCF7 Cells
Mashael Alhumaidi Alotaibi
This study investigated the potential inhibitory effects of lemongrass leaves extract on the proliferation of MCF7 breast cancer cells and its underlying mechanisms. MCF7 cells were exposed to increasing concentrations of lemongrass leaves extract (0, 50, 100,…
3 Mar 2025DOI 10.17582/journal.pjz/20230730015406- Research Article
Long Term Effects of SSRI Antidepressants on Levels of Different Serum Growth Factors in Patients with Depression
En Zhang, Ming Zhu, Wenli Zhu, Wei Xiong, Zhongfei Huang, Xin Qiao et al.
The objective of this study was to investigate the effect of long-term use of SSRI (selective serotonin reuptake inhibitors) antidepressants on serum nerve growth factor (NGF), glial cell line-derived neurotrophic factor (GDNF), vascular endothelial growth fac…
28 Feb 2025DOI 10.17582/journal.pjz/20230213200208 
Research ArticleBioinformatics Analysis of Promoter Methylation and Clinical Significance of Tumor Suppressor Gene HOXA5 in Oral Cancer
Haichao Wang, Yi Liu, Jingwen Wu, Wenjing Wang, Hongyi Zhang, Cunjian Yi
The HOXA5 gene is an important tumor suppressor gene, which has the function of regulating cell cycle, proliferation, and differentiation. Down regulation or inactivation of HOXA5 expression occurs in a variety of malignant tumors such as oral cancer (OC) and…
9 Mar 2025DOI 10.17582/journal.pjz/20230803165114
Research ArticleCDX1 Gene Expression in Recurrent Spontaneous Abortion under Extracellular Regulatory Protein Kinase1/2 Signaling Pathway
Shouyan Cao, Aili Yan, Wenhua Zhang, Fangfang Li, Xiaoning Liu
The aim was to explore the mechanism of the gene expression of caudal-related homeobox transcription factor 1 (CDX1) in recurrent spontaneous abortion (RSA) based on the extracellular regulatory protein kinase 1/2(ERK1/2) signaling pathway. In this study, 25 n…
20 Mar 2025DOI 10.17582/journal.pjz/20231030152055
Research ArticleEffect of Somatostatin Combined with Modified Dachengqi Decoction on Intestinal Function and Inflammatory Factors (TNF-α, IL-6, and IL-8) in Acute Pancreatitis
Hong Sun, Jie Shao
This article aimed to evaluate the effect of somatostatin combined with modified Dachengqi decoction on the improvement of intestinal function in patients with severe acute pancreatitis (SAP), and to explore its effect on the levels of inflammatory factors. Si…
20 Mar 2025DOI 10.17582/journal.pjz/20230804172924
Research ArticleEfficacy of Direct-Acting Antiviral in Treatment-Naive Chronic HCV Patients and in Those Previously Treated with Interferon and Ribavirin
Saima Younas, Quratulain Maqsood, Aleena Sumrin, Muhammad Umer Khan, Nazim Hussain
Pakistan has been ranked second highest in prevalence of hepatits C virus (HCV), all over the world. HCV genome depicts huge genetic variability resulting in seven different HCV genotypes (GTs) including around 90 sub types. Direct acting antivirals had revolu…
22 Mar 2025DOI 10.17582/journal.pjz/20230818135820
Short CommunicationExpression of Hemoglobin and Fibrinogen is Correlated with the Attack of Acute Arterial Embolism of the Lower Extremity
Li Nanlin, Liu Huijun, Ma Han, Ma Yanshou, Lv Fengquan, Dong Xiangyuan et al.
The objective of this study was to analyze the expression of hemoglobin and fibrinogen in acute lower extremity arterial embolism and its correlation with acute lower extremity arterial embolism. One hundred and twenty patients with acute lower extremity arter…
23 Mar 2025DOI 10.17582/journal.pjz/20220720190753- Short Communication
Immunohistochemical Analysis of P53 Gene Expression and other Protein Indicators in Chinese Hepatocellular Carcinoma Patients
Huifen Li
The objective of this study was to analysis P53 gene expression and other protein indicators in patients with hepatocellular carcinoma (HCC) in China. The present study collected a paraffin-embedded tissue sample of 31 patients with HCC from February 2021 to J…
23 Mar 2025DOI 10.17582/journal.pjz/20230613060647 - Short Communication
Effect of Labetalol Combined with Nifedipine on the Efficacy, Renal Function and Hemodynamics of Hypertension in Pregnancy
Xia Huang, Jin Guo, Zhijun Fan, Yue Xiao, Lifang Li, Yuehua Luo
The objective of this study was to investigate the efficacy of labetalol in combination with nifedipine and its impact, renal function and hemodynamics of pregnancy-induced hypertension (PIH). The sample of ninety-eight patients diagnosed with PIH and treated…
24 Mar 2025DOI 10.17582/journal.pjz/20230916184025 
Research ArticleMicroRNAs hsa-miR-222-3p, hsa-let-7b-5p, and hsa-let-7f-5p Regulate Their Putative Target Genes HMGA1 and CDKN1B in Pakistani Breast Cancer Patients
Hafiz Muhammad Farooq Yaqub, Ali Raza Awan, Rashid Saif, Sehrish Firyal, Muhammad Tayyab, Muti-ur-Rehman Khan et al.
MicroRNAs are the class of noncoding small RNAs that have the capability to control gene expression by degrading mRNA or repressing the post-transcriptional process. In the current era, role of microRNAs is one of the promising diagnostic, prognostic and thera…
4 Mar 2025DOI 10.17582/journal.pjz/20230502070537
ReviewClinical Expression Analysis of Serum miR-29b and miR-126 in Patients with Non-Proliferative Diabetic Retinopathy
Yan Liu, Xiao Zhang
Circulating miRNA remains stable and can affect glucose and lipid metabolism, inflammatory response, and angiogenesis pathways in diabetic patients by participating in energy metabolism, insulin synthesis, transportation, and signal transduction. It plays a cr…
23 Mar 2025DOI 10.17582/journal.pjz/20230525100537
Research ArticleGenetic Predisposition of PON1 L55M (T172A) and Oxidative Stress in Breast Carcinogenesis among Pakistani Population
Syeda Abiha Zehra Jaffari, Tuba Abid, Muhammad Zohaib, Ghulam Haider, Zehra Hashim
Breast cancer has become a global health issue across the world including Pakistan. In 2020, an estimated five-year prevalence of breast cancer is 30.1% depicting the major cause of socio-economic burden in our country. Owing to the fact that oxidative stress…
31 Jan 2025DOI 10.17582/journal.pjz/20221130081107
Research ArticleEffect of Remimazolam Combined with Propofol Anesthesia on Perioperative Cellular Immunity in Patients Undergoing Laparoscopic Ovarian Cyst Surgery
Xintao Dai, Zhenfeng Cai, Xingli Jiang, Pinglei Wu
The aim of this study is to investigate the effects of remimazolam and propofol anesthesia on perioperative immune cell levels in patients undergoing laparoscopic ovarian cyst surgery (LOCS). The sample of 90 LOCS patients was randomly divided to an experiment…
1 Feb 2025DOI Effect-of-Remimazolam-Combined-with-Propofol
Research ArticleAnalysis of Influencing Factors of Different COVID-19 Vaccinations on COVID-19 Infected Patients in Shanghai
Chonglin Yang, Cuiping Yang, Jingwen Jiang, Haifang Lai, Wenqiang Xia
Vaccination with COVID-19 vaccine can reduce the incidence of severe coronavirus infection and death. Different vaccinations have different impacts on patients who suffered COVID-19. However, the correlation between the dose and times of vaccination and the na…
31 Jan 2025DOI 10.17582/journal.pjz/20230304060316- Research Article
Predictive Study of Serum Matrix Metalloproteinase-8 in Combination with Human Chorionic Gonadotropin in Preterm Amniotic Cavity Infection
Yashu Lv, Yueyue Gao, Jinyi Wang, Yunchun Liu, Guiqing Jiao
The objective of this study was to investigate the predictive value of serum matrix metalloproteinase-8 (MMP-8) combined with human chorionic gonadotropin (hCG) for preterm amniotic cavity infection. The study included ninety patients with preterm labor admitt…
28 Feb 2025DOI 10.17582/journal.pjz/20230214050230 - Research Article
The Value of Procalcitonin Combined with C-Reactive Protein Assay in the Early Clinical Diagnosis of Bloodstream Infection
Cheng-Hong Chen, Ling-Xian Ding
This study aims to investigate the application value of procalcitonin (PCT) paired with C-reactive protein (CRP) in the early diagnosis of bloodstream infection. This study involved 298 patients with suspected bloodstream infections who were hospitalized in ou…
1 Mar 2025DOI 10.17582/journal.pjz/20221011021005 
Research ArticleAdvancement of KIF20B in the Process of Invasion and Metastasis of Colon Cancer Induced by EMT Through Gli1
Wenfeng Lin, Qiqi Zheng
The aim of this study was to determine the role of KIF20B in the metastasis of colon cancer cells. CCK-8 method and Transwell cell test were used to determine the proliferation, invasion, and migration of cells. Western blotting was used to determine the chang…
3 Mar 2025DOI 10.17582/journal.pjz/20230118070141
Research ArticleAllele Frequency Distribution of CYP2C19*3 and CYP1A1 Allelic Variants Associated with Clopidogrel Resistance in Cardiac Patients of Pakistan
Kashif-ur-Rehman, Ahmad Bakhsh, Muhammad Akram Tariq, Muhammad Khalil Ahmad Khan, Sumaira Mehboob
Drug resistance is a phenomenon that has received serious attention nowadays in medical practice. Such is the case with clopidogrel treatment response, which is variable inter-individually and inter-ethnically due to genetic polymorphisms in Cytochrome P450 (C…
8 Mar 2025DOI 10.17582/journal.pjz/20230613100642
Research ArticleIdentification of Novel Homozygous Varaint c.899T>G, p.(Leu300Arg) in DNAAF2 Gene Causing Infertility in Chinese Patient
Xiao Li, Wen Liu, CaiYi Chen, Shaoming Lu
The main aim of the current study was to investigate the Chinese patient having Primary ciliary dyskinesia (PCD). In the current genetic study, a Chinese patient was enrolled who was clinically diagnosed with PCD, and his family members were inquired for pedig…
8 Mar 2025DOI 10.17582/journal.pjz/20230413070451- Research Article
Analysis of High-Risk Factors of Stillbirth Among Pregnant Women in China
Xiaobo Wang, Haiyang Tang, Haizhen Dai, Qingyi Xiang, Jing Fang, Xiaoxia Bai
The objective of this study was to explore the high-risk factors of stillbirth after the two-child policy was fully opened. 327 cases of stillbirth were collected in the Obstetrics and Gynecology Hospital of Zhejiang University from January 2015 to December 20…
9 Mar 2025DOI 10.17582/journal.pjz/20230630110601 
Research ArticleRelationship between Serum sEng, PLGF, Gal-1 Levels and Pregnancy Outcome in Pregnant Hypertensive Mothers during Pregnancy
Yanfang Wu, Lei Chen, Xiaoning Jia
The objective of this study was to observe the relationship between serum levels of soluble fms-like tyrosine kinase-1 (sEng), placental growth factors (PLGF), and galectin-1 (Gal-1) in hypertensive pregnant women during pregnancy and the pregnancy outcome. Th…
9 Mar 2025DOI 10.17582/journal.pjz/20230809065557
Research ArticleProtective Effect of Nicorandil Injection on Myocardial Damage in Patients with Acute ST-Elevation Myocardial Infarction
Fuyi Liu, Qian He, Luling Luo, Qiong Mu
ST-elevation myocardial infarction (STEMI) is a serious cardiovascular disease. Nicorandil injection (NI) has the effect of dilating coronary artery and inhibiting calcium ion influx in myocardial cells, so it has a protective effect on myocardial damage (MD).…
10 Mar 2025DOI 10.17582/journal.pjz/20230821193140
Research ArticleCelecoxib and Cisplatin Synergistically Inhibit Oral Cancer Cell Proliferation Via Modulating the NOTCH 1 Signalling Pathway
Zuhair M. Mohammedsaleh, Mamdoh S. Moawadh, Fayez M. Saleh, Mohammed M. Jalal, Abdulaziz S Al-Otaibi, Nizar H. Saeedi et al.
Oral squamous cell carcinoma is the sixth most common cancer worldwide. Though there are significant improvements in diagnosis and treatment, the outcome is still very low. Thus, the development of novel therapeutic approaches is needed. In this study, we aime…
19 Mar 2025DOI 10.17582/journal.pjz/20230901031929
Research ArticleTetrandrine Induces Apoptosis in HepG2 by Modulating Hippo Signalling Pathway
Fuling Wang, Changlin Yue, Hong Li, Wenjing Yu, Wenlan Li, Guosong Xin
Tetrandrine (TET) has been known to possess anti-cancer properties in wide variety of cancers, however, the underlying mechanism for hepatocellular carcinoma (HCC) have not been fully elucidated. We focused our investigation the effect of TET on programmed cel…
19 Mar 2025DOI 10.17582/journal.pjz/20230410080440- Research Article
Nodakenin Inhibits Aurka-Cxcl5 Axis Induced Autophagy and Apoptosis in Non-Small Cell Lung Cancer and Promotes Radiosensitivity of Cancer Cells
Xiaoming Wang, Rui Tian, Kunpeng Duan, Xu Zhang
The objective of this study was to explore the role of nodakenin inhibiting AURKA-CXCL5 induced autophagy, cell death and promoting radiosensitivity in non-small cell lung cancer (NSCLC). The expression of AURKA-CXCL5 was determined in HBE, A549, H460 and H129…
20 Mar 2025DOI 10.17582/journal.pjz/20231221082217 
Research ArticleAquaporin-9 Aggravates Lipopolysaccharides Induced Acute Lung Injury Via Facilitating M1-Like Macrophage Polarization
Jiajia Wan, Chi Zhang, Xiaodi Li, Longkang Liu, Linxiu Peng, Yuejian Liu et al.
Alveolar macrophages (AMs) play a crucial role in orchestrating lung inflammation in acute lung injury (ALI), which largely depends upon M1-like macrophage polarization. However, the underlying regulatory mechanisms remain incompletely elucidated. The present…
21 Mar 2025DOI 10.17582/journal.pjz/20240502043333- Short Communication
Effect of Angong Niuhuang Pills on Positive Mental Symptoms Caused by Acute Lacunar Infarction in Patients with Mild Alzheimer
Haixiao Zhang, Xilong Yang, Yinfeng Li, Binyu Xu, Juan Du, Ronghua Wu et al.
The aim of this study was to explore the effect of angong niuhuang pills (ANP) on positive mental symptoms caused by acute lacunar infarction in patients with mild alzheimer (MA). A total of forty patients with MA from our hospitals between December 2021 and N…
23 Mar 2025DOI 10.17582/journal.pjz/20230802163930 
Research ArticleExpression, Characterization and Purification of Latcripin-5 from Lentinula edodes Strain C91-3 and its In Vitro Anticancer Activities
Akbar Hussain, Syed Riaz ud Din, Kavish Hasnain Khinsar, Muhammad Noman Ramzan, Min Huang, Anhong Ning et al.
Lentinula edodes C91-3 is an edible mushroom with demonstrated medicinal activity against various types of cancer in vitro and in vivo. The gene for Latcripin-5 (LP-5) was obtained from Lentinula edodes strain C91-3 and inserted into a pET32a (+) vector, which…
22 Mar 2025DOI 10.17582/journal.pjz/20230423120401