Effect of Low-Molecular-Weight Heparin Calcium on Senile Diabetes Nephropathy

The sample for this study consisted ninety consecutively admitted patients with DN at our hospital between March 2020 and October 2022. Patients in both groups were eligible to join the study if they were clinically diagnosed with DN based on symptoms, signs, and laboratory tests (Zhou et al., 2021), had good adherence to treatment, had complete clinical records, and were aged 65 years or older. The patients with severe diabetes complications, such as ketoacidosis, with other endocrine disorders, with severe heart or liver dysfunction, with malignant tumors, with known allergies to the medications used in this study, and with other urinary system disorders were excluded from the study.

Two groups, control group (CG) and observation group (OG), each of 45 patients were recruited. The CG received a routine treatment, including dietary adjustment, essential amino acid, high-quality low-protein diet, and adequate caloric intake; infection was controlled and anemia was corrected, blood pressure, diuretic, and glycemic control, correction of water, electrolyte, and acid-base balance disorders, and active treatment of the primary disease, among others. The OG group received subcutaneous injection of low-molecular-weight heparin calcium (National Drug Approval Number H19990077, Changshan Biochemical Pharmaceutical Co., Ltd., Jiangsu), 5000 U per injection, once daily, continuously for 8 weeks.

Three milliliters of fasting peripheral venous blood were collected from all study subjects, and serum was separated at 3500 r/min for 10 min and stored at -80°C. Serum levels of tumor necrosisfactor-α (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), serum creatinine (Scr), creatinine clearance (Ccr), blood urea nitrogen (BUN), indoxyl sulfate (IS), p-cresyl sulfate (PCS), chemokines, and adiponectin were determined using enzyme-linked immunosorbent assays (Varioskan LUX fully automatic enzyme immunoassay instrument, Thermo Fisher Scientific, USA). Fibrinogen (FIB), activated partial thromboplastin time (APTT), platelet count (PLT), and partial thrombo plastin time (PT) were measured using an automatic coagulation analyzer (ACL TOP 700, Werfen, USA).

Data were processed using SPSS 20.0. For categorical data, the chi-squared test was applied, while numerical data were presented as mean ± standard deviation (x̅±s). t-test was applied, with significance of P<0.05.

The gender distribution was 21 males and 24 females, rage from 66 to 82 years with an average age of 70.34±10.62 years in the CG and 22 males and 23 females, aged 65 to 81 years, with an average age of 71.72±11.20 years in the OG. While the duration of illness was 1 to 12 years with a duration of 4.64±1.62 years, it was 1 to 13 years with a duration of 4.50±1.03 years in the OG. There were no statistically significant differences in the general characteristics of the two groups (P>0.05), ensuring their comparability. Table I demonstrates that the clinical effectiveness rate in the OG was markedly superior (P<0.05).

Prior to therapy, there existed no notable variance in the kidney function metrics among the patient cohorts (P>0.05). Following the intervention, both groups displayed markedly reduced BUN and Scr levels compared to their pre-treatment measurements, with the OG exhibiting significantly superior outcomes (P<0.05). Additionally, Following the treatment, there was a substantial improvement in CCr levels in both cohorts, with the OG exhibiting a marked superiority over the CG to a significant degree (P<0.05) (Table II).

Following the therapeutic interventions, both sets of patients exhibited notably elevated PT and APTT levels in comparison to their pre-treatment values, with the OG surpassing the CG to a significant degree (P<0.05). Furthermore, the levels of FIB and PLT markers registered significant reductions from their pre-treatment levels, with the OG displaying a markedly superior outcome (P<0.05) (Table II).

Prior to the initiation of treatment, there existed no discernible statistical contrast in the presence of protein-bound toxins between the two patient cohorts (P>0.05). However, subsequent to the therapeutic regimen, both groups experienced a substantial reduction in the levels of IS and PCS, with the OG demonstrating a pronounced superiority (P<0.05) (Table II).

Prior to the commencement of therapy, no noticeable statistical divergence was evident in inflammatory markers (P>0.05). Nevertheless, following the treatment regimen, both groups experienced a marked reduction IL-6 and

Table II. Effect of low molecular weight heparin calcium on various biochemical indicators of nephropathy in patients with diabetes.

BUN, blood urea nitrogen; Scr, serum creatinine; CCr, creatinine clearance; PT, partial-thromboplastin time; APTT, activated partial-thromboplastin time; FIB, fibrinogen; PLT, platelet count; IS, indoxyl sulfate; PCS, p-cresyl sulfate; TNF-α, tumour necrosis factor; IL, interleukin. Notable Outcome: Marked reduction in Scr and BUN values by ≥ 20%, accompanied by amelioration in clinical indications and manifestations; Moderate Outcome: Scr and BUN levels reduced by 10% to 20%, along with alleviation of clinical symptoms and signs; Unsuccessful Outcome: Scr and BUN levels remain unchanged, and clinical symptoms and signs show no improvement.

TNF-α, as evidenced by a significant decrease (P<0.05), with the OG displaying a notably superior outcome (P<0.05). Additionally, the IL-10 levels saw a significant elevation compared to their pre-treatment values (P<0.05), with the OG surpassing the CG to a significant extent (P<0.05) (Table II).

Prior to the commencement of therapy, there was a lack of statistically notable variance in the serum concentrations of chemokines and adiponectin between the two sets of individuals (P>0.05). Subsequent to the treatment regimen, both groups experienced a marked reduction in serum chemokine levels compared to their pre-treatment values, with the OG displaying a significantly superior outcome relative to the CG (P<0.05). Furthermore, serum adiponectin levels exhibited a significant increase in both groups (P<0.05), with the OG surpassing to a marked degree (P<0.05) (Table II).

The use of low molecular weight heparin calcium in DN patients has a favorable clinical effect, which is beneficial for improving coagulation parameters and renal function. Scr is a metabolic product of muscle in the body, primarily excreted through glomerular filtration into urine (Mikhailov et al., 2022). When patients experience renal parenchymal damage, glomerular filtration rate decreases, leading to elevated BUN and Scr, as well as decreased CCr. Therefore, urinary Scr, BUN, and CCr are important indicators for diagnosing renal dysfunction and assessing treatment efficacy. PCS and IS are gut-derived protein-bound toxins known to have clear toxic effects on multiple organs. When renal function is normal, these toxins are excreted from the body via the kidneys. However, in cases of severe renal impairment in DN patients, renal clearance rate decreases, leading to the accumulation of IS and PCS in the body. This not only accelerates renal fibrosis but also increases the risk of cardiovascular complications (Noori et al., 2022). Low molecular weight heparin calcium, prepared by depolymerizing regular heparin, has been found to not only regulate renal hemodynamics and reduce renal hypercoagulability but also act as a negatively charged molecule similar in structure to heparan sulfate on the glomerular basement membrane. It can help restore the anionic charge barrier of the damaged capillary basement membrane, effectively reducing albuminuria (Cassinelli et al., 2020). Negatively charged low molecular weight heparin calcium can also bind to endothelial cells, improve coagulation factor activity, inhibit the formation of microthrombi within glomeruli, ensure unobstructed renal blood flow, and thus effectively improve renal function. It also promotes the clearance of protein-bound toxins within the body, reducing levels of blood creatinine and urea nitrogen and slowing down the progression of renal failure (von Zur-Mühlen et al., 2019).

It has been suggested that chemokines also function as adipokines, and studies have found that serum chemokine levels may be related to oxidative stress associated with renal function impairment in DN patients (Zhao et al., 2020). This study’s findings suggest that post-treatment, the OG experienced substantial reductions in the levels of IL-6 and TNF-α compared to their respective pre-treatment levels. Simultaneously, IL-10 exhibited a marked increase relative to. Furthermore, following the treatment regimen, the OG displayed notable declines in serum chemokine levels when contrasted with their pre-treatment levels and those of the CG. Additionally, the serum adiponectin levels in the OG significantly escalated post-treatment in comparison to their pre-treatment values. The use of low-molecular-weight heparin calcium in of DN patients is beneficial for improving metabolic parameters, reducing micro-inflammatory responses, and regulating adipokines. Studies have found that low molecular weight heparin calcium can improve lipid metabolism. Furthermore, it can reduce the expression of nuclear factors and pro-inflammatory factors caused by endotoxins, exerting anti-inflammatory effects (Zou et al., 2019).

The use of low molecular weight heparin calcium in the treatment of elderly diabetic nephropathy patients helps improve renal function, clear protein-bound toxins from the body, enhance coagulation parameters and metabolic indicators, and mitigate micro-inflammatory responses within the body.

Thanks to the members from Shaanxi Kangfu Hospital, the group collected samples, obtained data, and theoretical guidance.

The study received no external funding.

The study was carried out in compliance with guidelines issued by ethical review board committee of Shaanxi Kangfu Hospital, China. The official letter would be available on fair request to corresponding author.

This study was approved by the Advanced Studies Research Board of Shaanxi Kangfu Hospital, Shaanxi Province, China.

The authors have declared no conflict of interest.