Cansu Akbulut1,*, Tuğba Kotil2, Burcu Öztürk1 and Nazan Deniz Yön1
...ical effect on zebrafish ovaries after exposure to TiO2 nanoparticles. Adult zebrafish individuals were exposed to 1, 2 and 4 mg/L TiO2 for 5 days, and then their ovaries were evaluated using light and transmission electron microscopy. Numerous degenerated follicles with cytoplasmic vacuolization, mitotic catastrophe in mitochondria , chromatin condensation, mitochondrial vesiculation and dispersion at ...
Abd El-Nasser Ahmed Mohammed*
...t study were to evaluate ovaries age effect on restoration function upon transplantation in mice and rats. Eighteen adult female rats were classified to three equal groups in addition to sex young females as donor of young ovaries. Similar classification was done using eighteen female mice and sex young females. The females of mice or rats were anesthetized for ovarian transplantation using (6.2 and 13.2 mg/kg BW) and (13.3 ...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool3, Fatima Ahmed4, Saiqa Tabassum3,6, Syeda Madiha3, Tahira Perveen3 and Saad Bilal Ahmed5
...elopment of cysts in the ovaries with resultant difficulties in reproduction. The present study was done on 75 rats divided randomly in 5 equal groups fed with rat chow, caged chicken meat, uncaged chicken meat, raw spinach and soybean for a period of six weeks. The levels of plasma cholesterol, progestril, estradiol, and androgens were estimated at the end of the experiment. The ovaries of the rats were collected, weighed a...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...collected from different ovaries and supplemented to the in vitro maturation (IVM) media with proportions 0%, 10%, 20% and 40%. Oocyte maturation was assessed in different treatments and then cumulus-oocyte complexes were used for in vitro fertilization. Relative quantitative expression of mRNA transcripts related to apoptosis (Bax and Bcl-2), embryo development (LAMA1, IL-6 and FGF4) and stress (HSPB1) were examined. The results showed that 10% ...
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
...d atretic oocytes in the ovaries of C. gariepinus.
...
Neelima Gupta
... cavity, musculature and ovariesof Channa punctatus (prevalence 24%, intensity 2 to 18 per fish). Light and Scanning Electron Microscopy (SEM) were conducted to demonstrate the surface anatomy of the worm. The worm is slender, elongated and cylindrical. The mouth is slit-like, surrounded by two lateral rows of somatic papillae, cephalic end was conical with 12 labial papillae arranged in two circles of 6 papillae each. Scanning electron microscopy on at...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
...s were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. After in vitro maturation (IVM) in Tissue Culture Medium-199 (TCM199) for 24 h and fertilization (IVF) in Tyrode’s Albumin Lactate Pyruvate (TALP) medium for about 20 h, the presumptive zygotes were randomly distributed into 6 culture groups; Group I: Synthetic Oviductal Fluid (SOF) medium alone, Group II: SOF + co-culture, Group I...
Riaz Hussain Pasha1*, Muhammad Zubair Anjum2, Imran Ullah2, Muhammad Akram Khan3, Adnan Ali4, Saif-Ur-Rehman5, Sana Batool2 and Arslan Emmanuel2
...n cortex of cytoplasm in ovaries. Gonads attain maximum size and weight in spawning phase during summer. Free oozing of spermatozoa in testes and ovaries packed with fully grown eggs are the distinct features of this phase. The highest values of Gonadosomatic Index (GSI) for male and female in summer coincided with the histological structure of the gonads where they are in their spawning phase. Present study revealed that C....
Muhammad Younis1, Muhammad Irfan-ur-Rehman Khan1*, Mustansar Abbas1, Ali Murtaza1, Imran Mohsin2, Muhammad Shahzad3 and Muhammad Zahid Tahir1
Funda Turan1*, Ihsan Akyurt2 and Sehriban Cek-Yalniz1
... damage to the testes or ovaries. Therefore, this study suggested that red clover extract are effective in feminization of African catfish.
...
Haipeng Liu1, Xubin Jiao1, Xiangqing Li1 and Xinru Xu2*
...ign: justify;">Bilateral ovaries of 20 female SD rats were surgically resected. Four weeks after surgery, osteoporotic rat models were copied and randomly divided into a calcitriol group and a model group, with 10 rats in each group. The ovaries of another 10 rats were detached but not removed as a sham-operated group. After 12 weeks of continuous administration in each group of rats, the results showed that calcitriol incre...
Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1
...s detected in ear notch, ovaries, fallopian tubes and uterus of one cow (1/30) (PI animal). Whereas, BVDV antigen was detected only in the genital organs of acutely infected animals (2/30) but ear notch was negative. The positive BVDV antigen was clearly observed in the PI cow in the ovaries (primordial, growing, follicles, follicular fluid, granulosa cells, ovarian stroma and blood vessels), the fallopian tube (covering epi...
Othman E. Othman1*, Lingjiang Min2, Amira M. Nowier3
...e DNA was extracted from ovaries of Zaraibi goats belonging to two different fertility groups: high (HFG) and low (LFG) fertility groups. The extracted DNA was subjected to WGS after treatment with bisulfite. The findings declared that a small difference in the DNA methylation levels is present among high and low fertility groups. The methylated C frequencies in contexts: CG, CHG and CHH were 89.89%, 2.39%, 7.72% and 90.19, 2.34%, 7.47% in high and low fertili...
Abhishek Pandit1, Suman Poudel2, Manish Gautam3* and Shambhu Shah4
...l structure prevalent in ovaries in stages of estrus. Various protocols are available based on the hormone used, route of administration, requirements for heat detection, number of hormone injections, number of cattle handled, and injection time. Increased labor and upfront cost of hormone treatment, standard degree of supervision, and decent handling facilities are some of the drawbacks of this technique. Successful estrus synchronization necessitates optimum...
Samaa M Galal1, Sally Ibrahim2, Karima Mahmoud2, Ola Adel1, Aya A. Shokry3, El-Belely MS1, Ismail Sayed Taha1*
Widiastuti Ardiansyah1,3, Osfar Sjofjan2*, Eko Widodo2, Suyadi Suyadi2, Danung Nur Adli2
...he average weight of the ovaries, large white and small white follicles had a significant effect (p <0.05). In conclusion, increasing the level of curcumin produced a linear increase in the number of bioactive compounds and the reproductive organs of Mojosari ducks.
Keywords | Bioactive compound, Follicle, Mojosari Duck, Reproductive Organ, Turmeric
...
Maha Mostafa1, Sohail Soliman1, Reham I. Mohamed2 and Wael M. El-Sayed1*
... histology of testes and ovaries, causing a reduction in the sperm count and motility with a parallel reduction in the acid phosphatase activity. It also directly reduced the concentration of free testosterone and estradiol in both sexes without any apparent effect on LH or FSH hormones. It increased the total cholesterol in males only and elevated the triacylglycerols and glucose levels in both sexes. The administration of testosterone to males, females, or b...
Reem R. Tahon, Nora A. Shaker*
...m consisted of bilateral ovaries, oviducts, seminal receptacles, in addition to single vagina and gonopore, while the male reproductive system consisted of a pair of testes, vas deferens, and gonopods. The stomach was a complex structure whose walls bore calcareous ossicles. It was divided into the cardiac stomach with four ossicles: unpaired mesocardiac, paired pterocardiac, paired lateral zygocardiac, and the unpaired urocardiac ossicle; and the pyloric stom...
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
...ere obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml cysteine where 0 served as the control. IVF-TALP medium was used to fertilize the mature oocytes which subsequently underwent culturing in a simple culture m...
Ibrahim Elmaghraby*, Abdel-Baset I. El-Mashad, Shawky A. Moustafa, Aziza A. Amin
...documented, and positive ovaries were collected for further histological and immunohistochemical examination.Results: Interestingly, our results diagnosed 15 cases of sex cord-stromal tumors (8.30 %) in the ovary of dromedary camels included; adult granulosa cell tumor (0.55 %), interstitial cell tumor (0.55 %), thecoma or fibrothecoma (1.10 %), steroid cell tumor-NOS (0.55 %), and granulosa-theca cell tumor (5.55 %). Specifically, all cases had distinctive hi...
Trinil Susilawati1*, Aulia Puspita Anugra Yekti1, Amir Firdaus2, Dinda Ayu Damayanti2, Rizki Prafitri1, Nanang Febrianto1, Kuswati1, Achadiah Rachmawati1, Sri Wahjuningsih1, Nurul Isnaini1
....65% and 87.5% of normal ovaries, Corpus luteum persistent at 4.35% and 8.34%, and ovarian hypo function at 0% and 4.16% in T1 and T2, respectively. In conclusion, Artificial Insemination using the double doses method did not significantly improve the pregnancy rate of dairy cows. ...
Sumbul Zulfiqar and A.G. Rizwana*
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
...re media. The study used ovaries that came from slaughterhouse and were oocytes mature before fertilization using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and embryo culture media were collected using a disposable syringe and centrifuged, then supernatant in the collection, stored ...
Erni Damayanti1, Herry Sonjaya2*, Sudirman Baco2 and Hasbi Hasbi2
...ali cattle is to utilize ovaries from the slaughterhouse by using assisted technology, namely in vitro embryo production. This study aims to determine the developmental ability and to measure the concentration of hydrogen peroxide (H2O2) in Bali cattle embryos produced in vitro. 542 oocytes were matured in TCM 199 medium, were fertilized and were cultured using CR1aa medium for 48 h. After 48 h of culture, the embryo developed into 2, 4, 8 and 16 cells. Furthe...
Khamiss l , Omaima; El Helaly , Alexandra and Abol Ela 3, Said
...iginated from the insect ovaries tissues was established, cloned and maintained as contentious cell line. Nine serial dilutions of Polyethylene glycol (PEG) were prepared from mother suspension (18.2g PEGS" in 100 ml double distilled de-ionized water) started fromlO 1-109. Each concentration was prepared to treat five replicates of C35 cell dish. Treated cells were observed daily and counted 5 times within 15 days, and cell viability was detected by using...
Cut Intan Novita1,2, Kartini Eriani3, Amalia Sutriana4, Tongku Nizwan Siregar5,6*, Ni Wayan Karja7
...ion media. A total of 18 ovaries were usedwhich was divided into two groups: the vitrification medium group without Bovine Pituitary Extract (BPE, GibscoTM) (1 ml PBS + 0.5 M sucrose + 30% ethylene glycol) and with BPE addition (1 ml PBS + 0.5 M sucrose + 30% ethylene glycol + BPE 30 μg/mL). Each group was separated into three vitrification times of 0, 7, and 14 days with three replications. The results showed that adding BPE to the vitrification medium inc...
Gokarna Gautam1*, Sajan Rokaya1, Bhuminand Devkota1, Saroj Sapkota2
...as indicated by inactive ovaries and below basal level (<1ng/ml) plasma progesterone concentration. In conclusion, there was a clear seasonality pattern of reproduction in buffaloes in subtropical regions of southern Nepal with the autumn being the good and the spring being the low breeding as well as calving seasons; and 53% of buffaloes during low breeding season were in true anestrous condition.
Keywords | Buffalo, Reproduc...
Muhammad Yusuf1*, Abd Latief Toleng1, Sahiruddin1, Masturi1, Hasrin1, Musdalifa Mansur2, Hendrikus Fatem3, Johan Koibur4
...udy showed that inactive ovaries of dairy heifers were relatively higher in comparison to both follicular and luteal phases (58% vs. 26% and 16%). After treating the heifers with either Heatsynch or Select-synch protocols for synchronizing the estrus, all animals become estrus as predicted in advance. The mean interval from PGF2α injection to onset of estrus was 42 and 43 h (P>0.05) in Heatsynch and Select-synch protocols, respectively. However, the m...
