Muhammad Ashraf Khan1, Hizbullah Khan1, Abid Farid2
Muhammad Mushtaq
E-mail | mushtaq@uaar.edu.pk
...n bait in delivering the lethal amount of strychnine alkaloid in controlling the Indian crested porcupine, Hystrix indica, during 2006-07. Indian crested porcupine is a severe forest and agricultural pest throughout southern Asia and the Middle East, including Pakistan. The results suggested that in case of pre-baiting trial, a 25% reduction in burrow activity was achieved in case of groundnut – maize (1:1) grain bait loaded with 2% strychnine alkaloid a...
Klara Fischer1*, Erika Chenais1,2, Emeli Torsson1, Jonas Johansson Wensman1
...> (PPR) is a potentially lethal, highly contagious viral disease of sheep and goats. Domestic sheep and goats are important species for the livelihoods of poor people in many developing countries. Within societies where PPR is now spreading, poverty is widespread and the disease is expected to have significant negative impacts on livelihoods. In resource-constrained marginalised societies, it is often difficult to collect disease data in conventional ways. Par...
Asim Gulzar1,*, Ather Maqsood1, Munir Ahmed1, Muhammad Tariq1, Muhammad Ali2 and Rahmatullah Qureshi3
...studies for toxicity, sublethal and antifeedant effect. The result showed that ethanol based extract was the most effective to control the H. armigera followed by the ethyl acetate. The sublethal concentration of ethanol based extract of C. colocynthis increased larval and pupal duration as compared with control. The percent pupation, adult emergence and pupal weight decreased in treated populations as c...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*
... was built to assess the lethality of the E.coli isolates through subcutaneous-infection. Out of 81 E.coli isolates, the most prevalent gene detected was ompA (90.12%), followed by ETT2 (69.14%), irp2 (54.32%), EAST1 (46.91%), CS31A (41.98%), estB (19.75%), eaeA (14.81%), estA (12.35%) and K88 (1.23%), while others were negative. The result showed that the main serotypes of E. coli...
Faiza Ambreen*1 and Muhammad Javed2
...system and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC50 were calculated and fish were exposed to these concentrations, separately in glass aquaria for 70 days at constant laboratory conditions. Peripheral blood erythrocytes were taken on 14, 28, 42, 56 and 70 day of exposure for the DNA damage assessment in-te...
Rimsha Nawaz1, Muhammad Javed1, Sidra Abbas1* and Safina Kousar2
...s exposed to various sub-lethal concentrations of iron chloride. The results revealed that peroxidase activity was increased significantly in both kidney and liver after exposure to iron chloride in all the treatments as compared to the control group. It was found that enzyme peroxidase had activity of 0.364±0.004 and 0.588±0.004 UmL-1 in the metal stressed fish kidney and liver, respectively while in control fish, the same for both th...
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...ibacterial activity. The lethal dose and toxicity determination of the color extracted from marigold showed that it is safe for consumption as no clinical symptoms were observed in rabbits after giving the maximum dose of 1000ml of color.
...
Mahmoud M. Al-Azzazy1,2 and Saleh S. Alhewairini1,*
...rations which had a high lethality to tomato russet mites. Hence, Huwa-San TR50 can be utilized in the Integrated Pest Management (IPM) program as an acaricide agent.
... 
Shagufta Andleeb*1, Shabana Shaukat1, Chaman Ara2
...l abnormalities by a sub-lethal dose of cadmium chloride and protective role of garlic (Allium sativum), to minimize the intensity of these toxicities. For this purpose, fertilized eggs of Gallus domesticus were randomly divided into four groups of forty eggs each. Control group was intact and untreated. Eggs of one group were injected with a sub-lethal dose of cadmium chloride (1.5 µg/egg) in albumin on ...
Hafiz Azhar Ali Khan1,*, Waseem Akram2, Sumi Lee3, Shakir Manzoor1, Syed Rajab Ayub1, Khalil Ur Rehman1, Shinawar Waseem Ali1, Muhammad Bilal Chattha1 and Sumaira Maqsood1
...oassays. Based on median lethal concentration (LC50) values, all the strains of S. oryzae and R. dominica were more susceptible to spinosad than T. castaneum strains. The resistance ratios (RRs) of field strains at LC50 values were in the range of 2.24 to 3.24 fold for T. castaneum, 3.33 to 9.00 fold for R. dominica, and 1.73 to 3.45 fold for S. oryzae. The results revealed a variation in suscepti...
Muhammad Khalid Mukhtar1,*, Hummaira Iqbal1, Hafiz Muhammad Tahir2, Haseena Ghulam Muhammad1 and Muhammad Irfan1
... of prey density and sub-lethal doses of two commonly used insecticides i.e., bifenthrin and chlorpyrifos, on the consumption rate of web weaving spider Cyrtophora citricola, was observed. Live spiders for the study were collected from citrus orchards of Sargodha and maintained individually in the laboratory. Both the control and insecticide treated spiders were offered larvae of Drosophila melanogaster in different densities. C. citric...
Huma Naz1,*, Sajid Abdullah2, Sidra Naz3, Sidra Abbas2, Wardah Hassan2, Shakeela Perveen2, Moazama Batool1, Laiba Shafique2
...96 h LC50 and lethal concentration of Fe+Ni+Pb+Zn mixture for the fish, Cirrhina mrigala was determined in this research work. The fish were exposed to each of the 19 different concentrations (5-90 mg/L) of Fe+Zn+Pb+Ni mixture to determine 96 h LC50 and lethal concentration. During acute toxicity test, behavioral changes and catalase activity in liver, kidney, gills, brain and muscle of the fish ...
Farah Rauf Shakoori1,*, Anum Feroz1, Ayesha Gondal1,Sahar Akram2 and Tanzeela Riaz3
... in the godowns. The sub lethal dose of λ-cyhalothrin (LC20) significantly increased the contents of free amino acids and soluble proteins while contents of total proteins, lipids, glycogen and trehalose were significantly reduced with reference to their control (untreated group). Among carbohydrate metabolizing enzymes, the activities of amylase and invertase were significantly reduced while activity of trehalase were significantly increased...
Sukirno Sukirno1,2, Muhammad Tufail1,3,*, Khawaja Ghulam Rasool1, Said El Salamouny1, 4, Koko Dwi Sutanto1 and Abdulrahman Saad Aldawood1
...rformed to determine the lethal concentrations of this virus against the first and second larval instar of S. exigua. The potency of ten plant extracts as UV absorbers were done by measuring the absorption spectra of the 0.5% extracts using UV spectrophotometer. Based on preliminary data, clove, green tea and henna extracts at 10% (v/v) concentration were added to the virus and tested under semi-field conditions on the cabbage plantations for 7 days. Th...
Farah Rauf Shakoori1,*, Tanzeela Riaz2, Uzma Ramzan1, Anum Feroz1 and Abdul Rauf Shakoori2,3,*
...n, respectively. The sub lethal dose of esfenvalerate (LC20) significantly decreased the contents of glycogen, glucose, trehalose and free amino acid while total protein and total lipid contents, were significantly increased with reference to their control (untreated group). Among carbohydrate metabolizing enzymes, the activities of trehalase, amylase and invertase were significantly reduced after treatment with sub lethal
Wajid Ali1,*, Asma Karim1, Muhammad Irfan2, Hafiz Abdullah Shakir3*, Ghulam Mustafa4, Zeeshan Shafique1, Ijaz Anwar1
... were exposed to sub lethal concentrations of deltamethrine. Fish were divided into 4 groups and each group contains six fish. Experimental groups viz. A, B and C were exposed to 25, 50 and 75% of LC50, respectively for four days. Blood samples for biochemical analysis (blood glucose, cholesterol and total protein) and gills for histology were taken after 96 hrs. After acute exposure to pesticide, significant changes were observed in seru...
Sana Rana1, Sajid Abdullah1, Huma Naz2* and Khalid Abbas1
...Fish were exposed to sub-lethal concentration (1/3rd of LC50) of DM+END mixture for 14-day. CAT was partially purified by using ammonium sulphate precipitation technique and kinetic characterization was also performed against different pH and temperature. CAT activity was measured spectrophotometrically at A240 nm. Results showed that highest CAT activity was noted from the crude extract of control fish gills (140.66±0.71UmL-1) as compared to DM+END exp...
Tanzeela Riaz*†, Farah Rauf Shakoori, Syed Shahid Ali
Toxicity of phosphine against tolerant and susceptible populations of Trogoderma granarium collected from Punjab, Pakistan
...exposed to different sub lethal concentrations of phosphine for 20 hours. Based on LC50 data, Gujranwala population was found to be most resistant to phosphine whereas Sargodha population was found the least resistant population. Gujranwala population required 113.5, 128.8 and 168.1% more phosphine concentration in 4th
Shagufta Andleeb*
Teratogenic potential of pyrethroids: a review
...atogenic potential of sublethal doses of different types of pyrethroids in various groups of animals in systematic way. On line available data interpretation through Pubmed Central NCBI, Google Scholar and Google was done to achieve the target and 122 references were found the most relevant in this perspective....
Zubair Ahmed#, Muhammad Farhanullah Khan, Habiballah Rana*
Toxicological effects of Haloxylon recurvum Bunge ex Boiss (Khar Boti) whole plant extract and novel insecticide chlorantraniliprole against maize weevil, Sitophilus zeamais Motschulsky
...niliprole
induced lethal effects in S. zeamais. Probit analysis revealed LD50 value for H.
recurvum plant extract were 16.47 μg/insect and chlorantraniliprole were 0.0358
μg/insect, after 24 hours. The result revealed that H. recurvum plant extract had
insecticidal potential against the stored grain pest, S. zeamais. Current study
suggests H. recurvum plant extract could be a good alternative against
conventional...
induced lethal effects in S. zeamais. Probit analysis revealed LD50 value for H.
recurvum plant extract were 16.47 μg/insect and chlorantraniliprole were 0.0358
μg/insect, after 24 hours. The result revealed that H. recurvum plant extract had
insecticidal potential against the stored grain pest, S. zeamais. Current study
suggests H. recurvum plant extract could be a good alternative against
conventional...
Madeeha Arshad, Asmatullah, Chaman Ara
Embryotoxic effects of sodium arsenate in Mus musculus
...nate) other than general lethality additionally can be a teratogenic
agent. Present research work is focused on the teratogenic impacts of sodium
arsenate given to gravid mice (Mus musculus). Different doses of sodium arsenate
18.25, 37.5 and 75mg/kg B.W. were administered orally at day 6 of gestation
(single exposure group) to 15 females/dose group along with one control group
having 15 females, which was given only distille...
agent. Present research work is focused on the teratogenic impacts of sodium
arsenate given to gravid mice (Mus musculus). Different doses of sodium arsenate
18.25, 37.5 and 75mg/kg B.W. were administered orally at day 6 of gestation
(single exposure group) to 15 females/dose group along with one control group
having 15 females, which was given only distille...
Shagufta Nighat1, Muhammad Sajid Nadeem1,*, Syed Israr Shah1, Amber Khalid1, Tariq Mahmood2, Ayesha Aihetasham3 and Muhammad Asif4
...se values were below the lethal levels. Within the regions and sexes no significant variations were found in metal concentrations; however within three bat species metal concentrations varied significantly. This study provides baseline data for future comparisons and management of bats and metal concentrations in environment.
...
Ghulam Ali1,3, Wopke van der Werf2 and Just M. Vlak1,
... thus not reveal any sub-lethal effects of exposure to the virus on food consumption and weight gain. There was no viral dose dependency observed in food intake or weight gain by infected larvae, suggesting there is no increased crop damage upon virus treatment but rather a decrease to be expected.
...
Muhammad Asad Saleem1,*, Mirza Abdul Qayyum1, Mudssar Ali1, Muhammad Amin2, Muhammad Tayyab1,3 and Sumaira Maqsood4
...and survival against sub-lethal doses of B. bassiana and biorational insecticide (Nitenpyram). Sugarcane sets as alternate to date palm stem were successfully used as diet for rearing RPW. Combination of entomopathogenic fungus (Bb) and Nitenpyram (Nit) were found more lethal to survival of RPW. A reduction in pupation and adult emergence was recorded in the combined treatments. Moreover, decrease in weight gain, fras...
Rahat Afza, Muhammad Afzal, Muhammad Zeeshan Majeed and Muhammad Asam Riaz*
...rey and the impact of sublethal concentrations of insecticides on intra-guild predation (IGP) and aphid consumption by coccinellids. Data revealed that in the absence of prey larvae of C. septempunctata exhibited more predation on C. transversalis while the predation of C. septempunctata on C. transversalis was relatively less in the presence of prey. The larvae of C. septempunctata were found stronger and competitive than la...
Fariha Latif* and Muhammad Javed
...96 h LC50 and lethal concentration. These acute toxicity tests were performed at constant temperature (30oC), pH (8) and total hardness (250 mgL-1). All the three fish species varied significantly in their sensitivity towards metals mixture with the C. catla beingsignificantly highly sensitive followed by L. rohita and C. mrigala. After the acute toxicity tests, fish were exposed to 2/3rd of thei...
Abdul Khaliq1, Muhammad Irfan Ullah1,*, Muhammad Afzal1, Akhlaq Ahmad2 and Yasir Iftikhar3
...il both at 15% were most lethal to LGB, weevil and red flour beetle after 72hours following by effect of Datura stramonium (L.) and Eucalyptus camaldulensis (Dehnh) with respect to control. Similar response was noted at 10% concentrations of all plants but E. Camaldulensis (5%) was least effective against all insects after control. Resurgence response in F1 generation in each experiment showed high multiplication rate at low con...
Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2 and Muhammad Anjum Zia3
...ohita exposed to sub-lethal concentration for 14 days was evaluated. After purification, specific activity of enzyme was 1750.13 and 1272.72 U mg-1 in kidney of metal exposed and control fish, respectively. The enzyme from metal exposed kidney was 2.68 fold purified with 58.73 % age recovery. Results of characterization showed that SOD had broad range of pH from 4 to 8.5 but show maximum activity at pH 7 and 7.5 for stressed and control fish, re...
Attaur Rahman1, Sadeeq ur Rahman1,*, Ashfaq Ahmad2, Abdul Sajid1, Muhammad Asif Hussain1, Mian Muhammad Salman1, Niamat Ullah Khan1, Kamran Khan3, Fazli Rabbani1, Muhammad Akbar Shahid4, Irshad Ahmad5 and Shakoor Ahmad6
...5/ml/bird (chicken lethal dose 50 (CLD50)) was injected to ostriches and broiler (control) and were clinically observed for 7 days. Our results showed that a peak cloacal temperature was recorded on day 3 and day 4 with a 2.3°F and 1.2°F maximum rise in broiler and ostrich, respectively. A decrease in feed and water intake was recorded in both broiler and ostrich from 116 g to 82 g/bird and from 235 ml to 172 ml/bird for broiler, and f...
Fatima Tufail1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...a exposed to the sub-lethal concentration (1/3rd of LC50) for 14 days. The CAT was partially purified by using ammonium sulphate precipitation technique. Results of partial purification demonstrated that the exposure of insecticides mixture decreased the hepatic CAT activity in fish (43.33±1.41 UmL-1) as compare to control (50.33±1.41 Unit mL-1). After desalting specific activity of hepatic CAT rema...
Tanzeela Riaz1, Farah Rauf Shakoori2,*, Hareem Mansoor1, Sana Khan1 and Mushtaq A. Saleem1
...signed to figure out the lethal concentration (LC50) of emamectin, abamectin and spinosad alone and in various conbinations such as abamectin:emamectin (1:1, 2:1, 3:1, 1:2 and 1:3) and abamectin:spinosad (1:1, 2:1, 3:1, 1:2 and 1:3) against two larval instars (4th and 6th) of MBDIN and Lahore populations of T. granarium. The LC50 of abamectin, emamectin and spinosad for 4th larval instars of Lahore ...
Maliha Ayub1, Muhammad Javed1, Fariha Latif 1*, Faiza Ambreen2, Komal Sabir1
...luate the effects of sub-lethal concentrations of manganese on antioxidant activity in the fish, Labeo rohita. Fish were acclimatized for two weeks in the cemented tanks prior to the start of experiment. Four groups (n=10) of one year old fingerlings of Labeo rohita were exposed to 96-hr LC50 and sub-lethal concentrations (2/3rd,1/4th and 1/5th of LC50) of ...
Aqsa Pervaiz1*, Rukhshanda Afridi1, Zahra Pervaiz1, Rabia Masood1 and Hira Pervaiz2
...and D were exposed to sublethal concentrations of methylmercury 0.044 mg/L, 0.055 mg/ L and 0.073 mg/L in tap water respectively. The fish exhibited tail and fin disruption, bulging of eyes, scales erosion, mucous secretion, hemorrhage, color change, slow down swimming, lake of balance, restlessness, swimming side-wise, retardation of opercular movement and quick sudden movements. The fish was anesthetized and liver were removed after 48 and 96 hours for histo...
Muhammad Aslam Farooqi*, Bakhtawar Irsa, Sajjad Ali, Asif Sajjad, Muhammad Waqar Hassan and Sohail Akhtar
...the toxic impact, median lethal concentration (LC50) of each tested insecticide was determined. The results obtained, revealed that all insecticides were highly toxic after 24 hours of exposure at maximum concentration. Acephate and lymda-cyhalothrin showed the maximum toxicity with their LC50 of 83.96 and 139.07 ppm, respectively while diafenthiuron and profenofos were moderate in toxicity with LC50 values of 150.53 and 169.42...
Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed
...h post-treatment. Median lethal concentration (LC50) and median lethal time (LT50) values were calculated for each treatment using log-dose probit analysis. Results revealed a mortality response of termites directly proportional to the extract concentration for all treatments. The floral extracts of basil (O. basilicum) and Tecoma (T. stans) exhibited maximum termite mortality (i.e. 55.5 and 50.0%, respectively) with minimum...
Chaman Ara1*, Asmatullah1, Zainab Riaz1, Memoona1, Asma Chaudhary2, Shagufta Andleeb2
...tidote group (AD). A sub lethal concentration containing 15ug/g B.W of BP was given with and without turmeric orally, dissolved in corn oil, used as vehicle too. Dissections were made on 31st day, twenty-four hours after the last dose administration. Results showed that continuous administration of butyl paraben (BP) had decreased the organs weight as compared to control. Regarding biochemical parameters results had shown an increased enzymatic leve...
Hafiz Abdullah Shakir1*, Javed Iqbal Qazi2, Abdul Shakoor Chaudhry2, Muhammad Irfan3, Muhammad Khan1, Shaukat Ali4 and Saima Shahzad Mirza5
Mubasher Ahmad Malik1, Samina Jam Nazeer Ahmad2, Muhammad Jalal Arif1 and Jam Nazeer Ahmad1,*
...n of PxGV and AZA at sub-lethal doses exhibited increased larval, pupal and adult longevity and decreased larval and adult weight. In conclusion, combination of PxGV and AZA at low dose rate is a viable option to control diamondback moth.
...
Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*
...ing brine shrimp’s lethality assay. All the extracts showed significant biological activity against Gram positive, Gram negative bacteria and selected fungal strains. Acetone, chloroform and methanolic extracts showed maximum activity i-e 39mm, 44mm and 35mm followed by ethyl acetate 27mm and n-hexane 26mm against microorganisms studied. Standard antibiotics were used as a positive control for bacteria and fungi respectively. The cytotoxic assay results ...
Jam Nazeer Ahmad1,2*, Rashid Mushtaq1, Samina Jam Nazeer Ahmad1,2*Mubasher Ahmad Malik1, Mujahid Manzoor1, Muhammad Tahir2, Zubair Aslam4, Sumaira Maqsood5, Ishita Ahuja3 and Atle M. Bones3
...stic effect of three sub-lethal dose rates (2 x 103, 4.5 x 103, and 6 x 103 PIB/Larva) of native isolated Nucleopolyhydrovirus (NPV) from Spodoptera litura and Spinosad (0.01 ppm) against 3rd and 4th instar larvae collected from three different geographical areas of Punjab (Pakistan). A difference in larval mortality, pupation, adult emergence and egg ecluson was observed. The higher but sub-
Hamza Rehman*, Nida Ahmad Khan, Asad Ali, Warda Batool and Kanwal Razzaq
...at thiamethoxam was more lethal as compared to imidacloprid for concern insects. ...
Qaisra Siddique1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3
...ohita kept under sub-lethal dose (4.13 µgL-1) of chlorpyrifos. Fish was kept under chlorpyrifos stress for two months and samples were collected on weekly basis. It was noted that GST level varied significantly with duration. The GST level was raised in first 28 days after that it was dropped off up to 56-day. The trend of GST in fish tissues was observed as muscle<heart<brain<kidney<gills< liver. However, TPCs in selected t...
Anum Feroz1, Abdul Rauf Shakoori2 and Farah Rauf Shakoori1*
...ll;">Toxic effect of sub-lethal concentration (LC20) of bifenthrin, chlorpyrifos and their combinations was studied on the specific activities of esterases in the 4th and 6th instar larvae of susceptible laboratory strain (Lab-S) and deltamethrin resistant population (GUW) of stored grain pest, Trogoderma granarium. After exposure to LC20 of bifenthrin, chlorpyrifos and their combinations of 3:1 and 1:3...
Muhammad Shahid1*, Unsar Naeem-Ullah1, Waheed S. Khan2, Shafqat Saeed1 and Kashif Razzaq3
Application of Nanotechnology for Insect Pests Management: A Review
... the crops and cause the lethal effects on human health. Many countries in the world switched from chemical based agriculture to green based agriculture that are ecologically reliable, socially acceptable and economically sound. In current scenario, the nanotechnology has revolutionized the agriculture with the greatest potential of nano based insecticides for the insect pest management. The physical and chemical approaches are most widely used for the synthes...
Rahat Afza, Muhammad Asam Riaz*, Muhammad Afzal and Muhammad Zeeshan Majeed
Adverse Effect of Sublethal Concentrations of Insecticides on the Biological Parameters and Functional Response of Predatory Beetle Coccinella septempunctata (Coleoptera: Coccinellidae) of Brassica Aphid
...insecticides at their sublethal concentrations was assessed on the biological parameters and functional response of Coccinella septempunctata. All insecticides significantly reduced the fecundity, TPOP (total pre-oviposition period), larval weight and increased the life span of C. septempunctata relative to control. Thiamethoxam and lambda-cyhalothrin non-significantly reduced the coccinellids larval weight. Type II response was shown by larvae of C. septempun...
Simeen Mansoor, Jabeen Farheen* and Meher Hassan
...p;proteins induced by sublethal-temperature are molecular chaperons that positively regulate plant growth and development that govern acclimation in plants but little has been known under lethal-temperature stress. Thus, the impact of induction of thermotolerance by sublethal-temperature (40 °C), 100 µM indoleacetic acid (IAA), and 100 µM gibberellic acid (GA
Muhammad Arslan Ibrahim1*, Aneeqa Aleem2, Farkhanda Manzoor2, Shahbaz Ahmad1, Hafiz Muhammad Zahid Anwar1, Talia Aroob2 and Mansoor Ahmad3
... of fall armyworm. A sub-lethal dose of 4µl of entomopathogenic NPV suspension containing 4×106 polyhedral inclusion bodies (PIB) was tested as a mortality factor against the late larval instars of S. frugiperda when natural mortality tends to decrease after each successive instar. The single-sex method was adopted in the construction of the life table. The ratio of mortality in the late instars (3rd to 5th) was greater than the early instars becau...
Li Shengqing1,2,3, Zhang Xiyun2,3, Liu Shengcai2,3, Hu Guoyuan2, Fan Yuxia2,Liu Huaixin2, Wang Tingting2 and Zhang Yanming1*
...informatic analyses. The lethal median dose (LD50) values of intragastrically and orally administred type D botulinum toxin in wild plateau pikas, plateau zokors, and Microtus fuscus are calculated through Horn’s method and the improved Karber method. The ability of the toxin to prevent and control rodent damage is assessed through plot experiments. The safety of type D botulinum toxin toward nontarget animals, such as yaks, Tibetan she...
Chaman Ara1*, Asmatullah1, Saima Hanif1, Shagufta Andleeb2, Beenish Zahid3 and Madeeha Arshad4
...and exposed to three sub-lethal concentrations (37.5µg/g; 18.7µg/g; 9.37µg/g B.W) of aluminum, with and without onion extract. Control and antidote groups were treated with distilled water and onion equous extract, respectively. Doses were administered orally by gavage for 30 consecutive days, once a day and mice were euthanized on the 31st day. Blood was collected in serum separating tubes through intracardiac puncture for biochem...
Saima Naz1*, Ahmad Manan Mustafa Chatha2, Saba Saeed1
...toxicity (96-hr LC50 and lethal concentrations) for two fish species viz. Labeo rohita and Cirrhinus mrigala was determined in this study. During metal stress trials both species were kept at constant temperature (32°C), pH (7) and hardness (250 mg L-1) of water. Physico-chemical parameters of test medium were monitored regularly. The observed mean LC50 and lethal concentrations for Cirrhinus mrigala were 55.85 ± ...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...tion (73.3%) against the lethal A/chicken/Egypt/SHAH-1403/2011 (H5N1) AIV challenge, with no evidence of virus shedding. Keeping in mind that each bird was potentially challenged with 105 EID50, which may not be available to all birds under field condition. The foremost vaccine regimen can provide a safe, effective and considerable tool for the control of HPAIV in commercial chickens. Maximizing the beneficial role of recombinant and reassortant vaccines in si...
Abeera Razzaq1, Sajid Abdullah1, Huma Naz2*, Khalid Abbas1, Laiba Shafique3 and Qingyou Liu3
... exposed to the four sub-lethal doses (26.61, 13.30, 19.98 and 7.98 mgL-1) of Cr+Cr mixture for 28 days and blood was sampled after 7 days interval. A group (n=10) of fish were also kept in clean water (negative control=NC) and cyclophasphamid (positive control=PC), separately. Blood from caudal vein of fish was collected to see the micronuclei (MN) and de-shape nuclei (DN). Results showed that test dose 26.61 mgL-1 induced significantly ...
Lethal and Sub Lethal Effects of Plant Extracts and Green Silver Nanoparticles against Culex pipiens
Abdul Jabbar1, Muhammad Tariq1*, Asim Gulzar1, Tariq Mukhtar2 and Tayyaba Zainab3
... and concentrations. The lethal and sub lethal effects of plant extracts on the mean development of larvae was the maximum by A. indica while the effects on pupal period were the maximum by A. indica and Z. officinale. Similarly, female longevity was the maximum by A. indica and that of male by S. aromaticum. As regards green synthesized AgNPs, the lethal and sub l...
Ramy S. Yehia1,3, Essam A. Shaalan2* and Hashem M. Al-Sheikh1
... well as influence of sublethal dose from both fungi on the larval development were also evaluated. B. bassiana was more potent than M. anisopliae in both larvicidal activity and inhibition of flies’ emergence. Blends from fungi and essential oils exhibited synergistic effect but fungi mixture produced antagonistic effect. The development of M. domestica larvae was affected by sublethal dose from fungi. In conclusion, ...
Qaisra Siddique1*, Sajid Abdullah1, Huma Naz2, Khalid Abbas1, Laiba Shafique1 and Qingyou Liu3
...tify;">In this work, sub-lethal (1/3rd, 1/5th and 1/6th of LC50) effects of chlorpyrifos (CPF) on biochemical markers such as glutathione S-transferase (GST) activity and total protein contents in various tissues (gills, hepatic, renal, brain, muscle and cardiac) of Labeo rohita was determined. Fish was exposed for 60-day and sampling was done after 7-day. Results showed that the GST activity was considerably increased in L. rohita as compared to control. The ...
Samia Azad1, Iram Liaqat1*, Riffat Iqbal1 and Uzma Rafi2
...o rohita) exposed to sub lethal concentrations of heavy metal cadmium (Cd). CdCl2 was used as cadmium (heavy metal) source. Five concentrations of CdCl2 (0.2, 0.4, 0.6, 0.8 and 1.0 ppm of Cd) were tested in glass aquaria having 35 Rohu fingerlings in each. After exposure for six week, sections of intestine, kidney and liver were excised and studied histologically. Results revealed variation in histological changes from mild to severe depending on concentration...
Saima Mehar1 and Salma Javed2*
... exposure at 1% with the lethal concentration (LC50) values 0.168 mg/land all four concentration are 100 % toxic at 72 h of exposure. W-56D proved to be 100 % lethal at 0.1 and 0.5 % concentration after 48 h of exposure with (LC50) 0.065 mg/l. The lowest mortality was found from WC-3 and the mortality rate was found to be 30-50 %with (LC50) values 0.135 mg/l, 0.187 mg/land 0.145 mg/lafter 24, 48 and 72 hrs of exposure. Compo...
Deepesh Kumar Bhuptani1, Atta Hussain Shah1*, Gul Bahar Khaskheli1, Zubair Ahmed Laghari2, Ghulam Shabir Bahram1, Muneer Ahmed Jamali1, Tarique Ahmed Khokhar1
...c carcinogen and is less lethal but it is still cytotoxic. The present study was conducted on prevalence and quantification of AFM1 in raw buffalo milk in Sindh province. A total of three hundred and fifteen raw milk samples (n=315) from dairy farms of three regions in Sindh province i.e., southern (Karachi, Thatta, Hyderabad), central (Mirpurkhas, Umerkot, Shaheed Benazirabad) and northern (Naushahro Feroze, Sukkur and Larkana) were collected and analyzed usi...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
...gher concentrations were lethal for the cancerous cells. IC50 was found to be 12.6 µM. Further contextualization of the therapeutic potential of G9a inhibition was provided by expression analysis of the G9a gene and genes associated with cell proliferation, tumor suppression, and apoptosis. The gene expression of fibroblast growth factor 1 (FGF1), enolase 2 (ENO2), cyclin D1 (CCND1), catalytic subunit of AMP-activated protein kinase (AMPKα2), RNA p...
Hamenya Mpemba1, Fan Yang1, Kirsty J. MacLeod2,3, Dusu Wen1, Yan Liu1,4 and Guangshun Jiang1,*
...iour of prey species via lethal (direct kill) or non-lethal effects (i.e., through predation risk). For example, prey species may move from areas perceived as risky to safer spaces where predation risk is lower, which can have important consequences for investment in foraging, movement, and mating, and for the behaviour and habitat use of other species, such as mesopredators. These changes in prey and mesopredator behaviours...
Nosheen Jehajo*, Nasreen Memon, Mansoor Ali Shah and Naheed Shah
... 0.50% of both oils were lethal concentrations (LC50) which killed almost (50%) population of beetles after 48 hrs of exposure. Furthermore, both oils were effective by reducing (100%) oviposition, population emergence and seeds infestation rate as compared to control in which 75% infested grains were found including the large number of eggs and adults. These two oils provided best protection at their higher concentrations to mung beans against Callosobruchus ...
Emtiaz Ibrahim Ghoniem, Gaber Mamdouh Abdelgalil, Amira Farouk Gad* and El-Sayed Hassan Eshra
... intoxicated with two sublethal doses of CuSO4 (0.25 and 0.5 of LD50) after 24, 48 and 72 h were examined. The results indicated that the LD50 values of CuSO4 were 166.5, 92.59 and 70.63 µg/g b.w for 24, 48 and 72 h, respectively. The biochemical effects of CuSO4 led to a significant increment of AChE activities in treated snails after all tested times. While the tested compound inhibited ALP activities in all treated animal groups. AST and ALT activitie...
Arif Mehmood1*, Muhammad Naeem2, Abu Bakar Muhammad Raza1, Muhammad Asam Riaz1, Muhammad Zeeshan Majeed1, Nadra Khan3 and Waqas Raza4
...s on the earth, transmit lethal diseases in humans as well as animals. Spatial distribution of mosquitoes provides clues for their precise and accurate control. In this study, which was conducted in 2014-16, different habitats including, graveyards, scrapyards, forest areas, streams, crops, residential areas, and animal sheds were specified in Jhelum district. Surveys for the collection of mosquitoes were made and a total of 365 specimens were collected. These...
Sayed H. Al Habty1, Dina N.Ali2*
H. M. Hassan† and S. A. Ibrahim
...gic smart at the quarter lethal concentration (LC25) is effective in causing mortality of S. littoralis....
A.M. El-Baha1, A. A. El-Sherbiny2†, M. Z. M. Salem1, N. Shaarawy3 and N. H. Mohamed3
...tively. In addition, the lethal concentrations (LC) causing 50% J2 mortality (LC50) of C. citriodora and E.
camaldulensis oils were 235.9 and 327.7 mg/l, for C. citriodora and E. camaldulensis leaves EOs, respectively.
Essetial oils were analyzed by gas chromatography-mass spectrometry (GC-MS). Isopulegol (53.68%), citronellol
(15.26%) and isopulegol acetate (15.25%) were found to be major constituents of C. citriodora, whil...
MARIUM ASLAM, MUHAMMAD JAVED, FAIZA AMBREEN* & FARIHA LATIF
...LC50 of zinc and its sub-lethal concentrations viz. 2/3rd, 1/4th and 1/5th of LC50 for 30 days at constant laboratory conditions. After 30-day, the fish were sacrificed and their liver and kidney were analyzed for peroxidase enzyme activity. Change in activity of peroxidase enzyme in the fish exposed to sub-lethal concentrations was compared with the control. Dose dependent increase in the activities of peroxidase enzyme was...
Dalia Mansour Hamed1, Mohsen Zaky El Dimerdash1, Ahmed Abdelatif Ali2, Samer Adel Abd El-Gaffar3, Mona Salim Abdallah1*
... was the predominant and lethal bacteria in young rabbits. In addition, the semi closed nursing system improved the health of newborn rabbits. finally, probiotic/antibiotic combination is the best choice against S. aureus infection. The guide lines in rearing systems , mainly semi-closed nursing system , would decrease the susceptibility to bacterial infections
Keywords | Antibiotic; Mortalities; Nursing; Probiotic; Rabbit; S. aureus.
...
Safaa Rhaimi1, Sara Brikat2, Mouloud Lamtai2*, Mohammed Ouhssine1
...rthermore, the LD50 (50% lethal dose) was determined at 3000 mg/kg. Based on these findings, the no observed adverse effect level of salvia officinalis essential oil was established to be 1000 mg/kg. Evaluation of the effect of this therapeutic dose revealed a clear anxiolytic and antidepressant actions and improved memory functioning in female Wistar rats. In conclusion, salvia officinalis essential oil produce neuroprotective activity in female rats.
...
Heavy metals (HMs) are harmful and lethal at negligible levels and non-biodegradable in the typical ecosystem and constitutes animal, human and environmental hazards. They are divided into toxic metals like Lead, Cadmium, Arsenic, etc. and essential elements like copper, zinc, manganese, iron, nickel and chromium. Additionally, could be categorized into two groups based on the natural and anthropogenic sources releasing origins. Population and industrial expansion led to food contamination with HMs. Poisonous metals can be transferred from irrigation water to agricultural soils, agricultural operations, air pollution, animal feed, and packaging materials. Toxic metals are non-biodegradable, non-thermos degradable, and exceedingly stable in the ecosystem; as a result, they quickly build in various foods. Metal pollution of many foods, including agricultural commodities, and animal protein sources such as fish, milk, meat, and eggs, poses a hazard to food safety and security. Toxic metal pollution of irrigation water, agricultural soils, plants, and animals result in their integration into the food chain, posing a health hazard to humans. Most metals are harmful to animals and humans and accumulate in several organs like the skeleton, hepatic tissue, spleen, and renal tissues. Metals have a deleterious impact on the production of plants and animals. As a result, several remediation strategies have become necessary to limit the hazardous HMs pathway into the food chain and the human body. Metal nanoparticles are employed in beneficial applications, although they are associated with specific hazards.
Keywords | Food contamination, Heavy metals, Nanoparticles, Pollution sources, Remedy, Soil contamination
...ls (HMs) are harmful and lethal at negligible levels and non-biodegradable in the typical ecosystem and constitutes animal, human and environmental hazards. They are divided into toxic metals like Lead, Cadmium, Arsenic, etc. and essential elements like copper, zinc, manganese, iron, nickel and chromium. Additionally, could be categorized into two groups based on the natural and anthropogenic sources releasing origins. Population and industrial expansion led t...
Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3
... a predetermined minimal lethal dose of gamma radiation (4krad kGy/min) and the bacterial inactivation was assured using bacteriological examination. Two experiments were done; in experiment No.1, the immunizing efficacy of the prepared vaccine was evaluated in two groups of apparently normal lactating cows (4 cows /group). The first group was unvaccinated and kept as a control group all over the study while the animals in the second group were immunized with ...
Muhammad Rizwan1*, Bilal Atta1, Muhammad Arshad2, Sohail Akhter3,
Muhammad Tahir3, Misbah Rizwan1, Muddassir Ali1 and Arshad Makhdoon Sabir1
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...c colitis (HC) and other lethal complications. Consumption of STEC contaminated food has been associated with food related illnesses outbreak. This study aims to evaluate the prevalence of O157:H7 strain of STEC by detection of Stx-1 and Stx-2 genes in 160 chicken meat samples, which are randomly collected from different regions of Northern Punjab, Pakistan. Isolation of pathogen from meat samples were performed with the use of International Organization for s...
Selvaraj Thanga Malathi¹, Venkatraman Anuradha1*, Mohamed Yacoob Syed Ali2, Muhameed Sajjad Sarwar3, Nagarajan Yogananth2
...niloticus) exposed to sublethal concentration of Lithium [30%] for a chronic period of 60 days. The levels of FT3, FT4 and TSH tested were significantly decreased in treated groups when compared to the normal. Thus, it is evident that determination of thyroid hormone secretion and histological studies (FESEM – field emission scanning electron microscopy) were useful in determining the effect of trace metal pollution and its effect in freshwater fish spec...
Bing Yan1, Meng Meng Zhang2, Xi Chen2, Yongming Li2*, Muhammad Khan3* and Tonghui Ma1*
...e of the most common and lethal primary brain tumors due to its high malignancy and heterogeneity. Despite recent advances in medical technology and concerted effort to improve therapeutic methods, the prognosis of GBM still remains very poor. The poor prognosis is associated with cancer stem cells (CSCs) which hold the potential of self-renewal, and differentiation into pathogenomic heterogeneous tumor formation leading to therapeutic resistance. Therefore, t...
Faiza Naeem1, Muhammad Farooq Sabar1, Muhammad Usman Ghani2,*, Qurat Ul Ain1 and Qurat-ul-Ain Zafar1
...eractions and centrality-lethality hypothesis representing that knockdown of influential node and edge leads toward the development of the disease. This ranking allows identification of the influential protein for the targeted drug discovery and therapies. An extensive study of published articles was conducted to enlist asthma-associated genes reported significant (P-value < 0.05) in the Pakistani population. Pathway Connector (http://bioinformatics.cing.ac...
Wei Wan1, Xiao-Li Wang1, Shu-Juan Wang2, Qin Si2 and Li-Qing Duan1*
...ica adult, with a median lethal concentration (LC50) value of 0.52 g/L. Among the 66 binary mixtures, 19 showed strong synergistic effects, while 21 showed antagonistic effects. The most profound synergistic effect was the mixture of l-carvone and dihydrocarvone, with an expected mortality of 35.2% and actual mortality of 98.4%. Estragole had the highest frequency of antagonistic effect (7 combinations), and the most significant antagonism was observed when co...
Rana Mahmood Ahmad*, Orooba Mohammed Maeed Ibrahim
...unds. Oil’s median lethal dose (LD50) was 2225 mg/kg, which suggests that clove oil has a relatively low level of toxicity. Eighteen adults male Wistar albino rats, six months, weighing 200–250g, were used and randomly grouped as follows: Group 1 was kept as a positive control group without treatment. At the same time, Group 2 received clove oil extract orally (10 mg/kg B.W.). Group 3 received Piroxicam (5mg/kg B.W) orally. The anti-inflammatory ac...
Eman, M. M. Soliman*; Taha, M. M.*;El-Sanousi A,;Shalaby M. S. Wasscl , Youscf Adel*
...rus 103 to 104 mouse I/P lethal dose50(MIPLD50), it was ranged from 0.006 - 0.0007. Using the Rt-PCR to confirm the efficacy of binary ethyleneamine as inactivator to RVF virus used in tested vaccine through applying RTPCR on master seed RVF virus as positive control samples, RVF virus after inactivated with binary ethyleneamine and on the eluted RVF antigen from the tested vaccine, the master seed RVF virus gave positive band at 800bp but the RVF virus ...
Xiaojun Li
...nd dihydrotanshinone had lethal effect on diamondback moth larvae, and the correlation coefficient was 0.972. Through further antifeedant test, it was found that diamondback moth larvae had obvious antifeedant behavior to acetone extracts of SIB represented by cryptotanshinone and dihydrotanshinone, and the correlation coefficient was -0.915. By further observing the influence of acetone extract of SIB on the feeding selection behavior of diamondback moth larv...
Asma Ilyas1, Tanzeela Riaz 2*, Sammi Rasheed1, Uzma Ramzan1, Afsheen Munawar Butt1 and Farah Rauf Shakoori1*
Hongsen Xu*, Xiaoni Wang, Tie Tian, Changyu Zhao, Denghang Yu and Jun Liu
...fected crayfish, and the lethal dose 50% was 1.52 × 106 CFU/g crayfish. Serious histopathological changes, such as cell degeneration and necrosis, hemorrhage and inflammatory cell infiltration, were found in diseased crayfish. In conclusion, this study confirmed the pathogen of whitish muscle disease in farmed P. clarkii, and preliminarily investigated the biological characteristic, pathogenicity and drug sensitivity of this bacterium. The results of thi...
Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee
...ere to assess the median lethal dose (LD50) of VD in female albino rats and to assess the effects of vitamin D (VD) on the severity of acute upper or chronic vitamin D intoxication (VDI) through the study of cytogenetic effects in addition to histopathological changes in the tissues of female rats. The methods included in the first experiment, the female rats were orally administered the following lethal doses: 0.5, 1, 1.5, ...
Moazama Batool1*, Saima Naz2*, Sheeza Bano1, Sadia Nazir3, Ghulam Abbas4, Ahmad Manan Mustafa Chatha5, Maria Lateef2 and Fatima Yasmin2
... determined by the LC50+ lethal concentration of a tertiary combination comprising lead (Pb), nickel (Ni), and cadmium (Cd) was assessed on two freshwater fish species, Catla catla and Labeo rohita, employing probit analysis. Over a period of 90 days, mortality served as the primary toxicity criterion. The experiments were conducted under consistent conditions of pH (7), temperature (28.00.00°C), and water hardness (198.00 mgL-1), with three replicates for...
SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3
...trains. Accordingly, sub-lethal doses of the drug result in development of hyper-resistances. The bacteria have evolved the molecular genetical basis (and other parameters) for acquiring the resistance. For the containment and eradication of globally evolving MDR bacteria, it is crucial to understand and implement certain strategies/agents such as, probiotics, CRISPRs, bacteriophages, nanotechnology and phytochemicals....
Muhammad Wasif Gulzar*, Riffat Maqsood, Muhammad Zain, Muhammad Suleman, Tayyab Ur Rehman, Sana Asif, Abdul Wadood and Jawad Hussain
...rough animals and can be lethal. It is a disease that affects all warm-blooded species and is widespread worldwide, except for islands like Australia and Antarctica. Rabies causes more than 60,000 deaths annually, although every year about 15 million people receive Rabies Post-Exposure Prophylaxis (PEP). Wildlife including raccoons, skunks, bats, and foxes are major rabies reservoirs. The disease is primarily spread by the bite of a rabid animal and the saliva...
