Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...aluated by intramuscular immunization of SPF chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chickens shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...aluated by intramuscular immunization of specific pathogen free (SPF) chickens with two different concentrations of Egy-H5 plasmid DNA 15µg and 60 µg. Results demonstrate that chickens developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmi...
Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1
... essential benchmarks of immunization and vaccine potency. ...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
...s observed regardless of immunization status. Our results suggest that vaccination of African wild dogs with a live attenuated domestic dog vaccine may provide adequate protection against CDV, and could therefore be an important tool for the conservation of this endangered species.
... 
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...ed at 2 weeks post final immunization. Results showed that mice both in T1 and T2 groups elicited significantly stronger anti-SS antibody level than that in control (p < 0.05), and mice in T1 group induced significantly higher serum GH and PRL concentration in comparison with T2 and control groups (p < 0.05). Expectantly, mice in T1 group also showed significantly higher average weight gain and offspring weaning weight than that in T2 and c...
Bo Wu1, Hui Zhang2, Kun Li2, Khalid Mehmood2,7, Yan Zhao1, Bin Jiang1, Chengjun Xue3, Muhammad Tariq Javed6, Fazul Nabi2, Zhaoqing Han5,* and Houqiang Luo4,*
... the sero-prevalence and immunization program optimization of serotype O of FMD through ELISA and IHA in pigs in Zhejiang. A total of 368 serum samples were collected from June to July in 2016. Out of these samples, 23 (6.79%, 95% CI 4.4-9.9) pigs were found positive for FMD antibodies with the further distribution of 9.09% (95% CI 4.6–15.7), 6.90% (95% CI 2.6–14.4), 5.49%, (95% CI 1.8-12.4) and 4.35% (95% CI 0.9-12.2) from Wenzhou, Lishui, Jinhua ...
Syeda Beenish Bukhari1, Sidra Khalid1*, Shahid Bashir1, Riffat Mehboob2, Humaira Waseem2
...etermine the barriers of immunization among mothers attending Pediatric and Gynecological Department of the Sir Ganga Ram Hospital Lahore. For this, 150 mothers were selected randomly; relevant data were collected through pre-tested questionnaire. Data were tabulated and analyzed with the help of SPSS version 21.0. Results of current study showed that barriers to complete vaccination course were lack of information, myths, un-availability, non-affordability, f...
Yan-Guo Han1,2, Jun-Hua Ye1, Qin-Tao Zhao1, Yong-Jie Huang2, Kai Li2, Yong-Fu Huang2 and Li Xu1,*
...collected before primary immunization and at weeks 3, 5 and 7 after primary immunization and body weight of offspring were weighed at weeks 0, 2 and 4 after birth. Both SS-14 and SS-28 DNA vaccines induced humoral immune response, however, antibody response in T1 group were significantly stronger than that in T2 and C groups. Serum GH levels in T1 group was significantly higher than those in T2 and C groups, an...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...es titer in rabbit after immunization in 100% pure serum sample was 0.736 which was 15 times more than that of pre immune control sera (0.049). It is concluded from the current study that purified HCV3a envelope glycoprotein E2 has significant antigenic activity and can potentially be used as recombinant vaccines against HCV infection.
...
Housh Muhammad Solangi1, Javaid Ali Gadahi1*, Mansoor Tariq2, Bachal Bhutto1, Zubair Ahmed Laghari1, Jamila Soomro3, Taufeeq Ahhmed Khosa1 and Abdullah G Arijo1
...d 5th week of immunization. Impact of the HcCP on haemoglobin level was also evaluated. After second week of infection significant difference (P<0.05) was found between the negative and positive control. However no significant difference (P>0.05) were found between vaccinated and control groups. Statistically significant difference (P<0.05) was observed among positive control group and vaccinated group at 3rd week of post in...
Aly M. Abdel-Salam
...he present study. Rabbit immunization was used for induction of antisera for CYSDV and CVYV. RT-PCR was used to amplify coat protein genes for the two viruses using specific primers. DAS-ELISA and immuno-blotting were used for evaluating the induced antisera. Results: Antisera for CYSDV and CVYV were produced efficiently and used for virus diagnosis through DAS-ELISA, DBIA, and TBIA. RT-PCR confirmed the nature of the two viruses. However mixed infection was n...
Sana Shakoor, Tahir Rehman Samiullah, Naila Shahid, Abdul Qayyum Rao*, Aneela Yasmeen, Sana Tahir, Ayesha Latif, Saira Azam, Ahmad Ali Shahid and Tayyab Husnain
...ity. The intraperitoneal immunization of mice with HA protein along with enhancer (ferund adjuvants) was done and produced antibodies in serum was detected by immunodot blotting assay. The appearance of the dot on spotted position confirmed the specificity of anti-HA antibodies. The titer of antibody produced in immunogenic mice was determined through ELISA. The highest geometric mean titer of HA-specific serum IgG, which was greater as compared to the control...
Manish Gautam1*, Pravakar Kattel2 and Krishna Kaphle3
...evention are possible by immunization, maintaining biosecurity in the herd, controlling the vector, and isolating the infected animals....
Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1
...; 0.13) at day 28th post immunization (p < 0.05). Challenged calves showed the lowest mean clinical respiratory sign score in booster-dose immunized calves (1.2 ± 0.35) than in the single-dose group (4.2 ± 0.51) but the highest score was noted in the control group (6.2 ± 1.01). Hence, the current finding revealed that booster-dose-based immunization was found to induce protective level of immune respo...
Soshe Ahmed1*, Mst. Ishrat Zerin Moni1, Maksuda Begum2, Md. Jafar Eqbal3, Md. Shahidul Islam4, Mst. Samira Tanjim1, Mst. Rokeya Sultana1
..., along with other basic immunization schemes. After 14 days of ND vaccination, post-vaccination sera samples were collected and subjected to an HI test to determine antibody titers. The Haemagglutination inhibition test was done using Newcastle Disease Virus LaSota strain NLT 106.0 EID50 antigen. Post-hatch feed and water deprivation durations significantly affect the production parameters. Feed intake, body weight gain and average daily gain were significant...
Salwa N. Zeinl and Hanaa S. Zawam2
...tion was used for rabbit immunization. The polyclonal antibodies raised against the local isolate of ACLSV had a specific titer of l: 8000. The concentration of IgG and IgG conjugate with alkaline phosphatase was l: 1000. The prepared antiserum was used for detection Of ACLSV using ELISA dot immunobinding assay (DIBA). The presence of the virus was checked in blossom parts. (Petioles, stigma, and flower cup)....
Rafik Soliman1*, Rafik H. Sayed2, Hanan Mahmoud3, Heidy Abo El-Yazeed1, Marsell Saad1, Shaimaa Abdelall Elsaady2, Khalid Sh3
...t 2 weeks intervals post immunization and/or antibiotic treatment. Vaccinated cows in the two experiments did not develop clinical mastitis during the whole observation period that extends up to 20 weeks post treatment. The total immunoglobulin concentration (g/dl) measured four weeks post immunization was significantly higher in the serum and milk whey of immunized cows as compared to the non-immunized cows (P<0.01). The...
Elamien***, Mohammed; Kotob**, Nahla S.; Elsayed*, Eltayeb A.; Ragab, Yasser M. and Mourad, Fathia E.
...lign: justify;">National immunization days (NIDs) started in 1994 in Sudan by using trivalent OPV (topV) and in the beginning of 2010 bivalent OPV (bOPV) was permitted to be use. Since OPV is manufactured from a highly thermolabile vaccine strain of poliovirus, it should be stored under the recommended temperature of -200C or below. A vaccine vial monitor (VVM) is a label containing a heat sensitive material which is placed on a vaccine vial to register cumula...
Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.
...V was obtained by rabbit immunization using three different methods of injections. The titer Ofthe prepared antiserum as determined by indirect ELISA with dilution of tissues 1/5 was 1/1024. Purification of the immunogamaglobulin G and conjugate of IgG with alkaline phosphatase were carried out to be used for ELISA detection. The concentrations of IgG and IgG conjugated with alkaline phosphatase were 1.0 mg/ml and 1:1000 respectively. Antiserum produced specif...
Laila,A. Sedeek; Fatma,S. Mohamed and Manal Abo El-Yazyed
...goats to the concomitant immunization with PPR and bivalent FMD (type A and O) vaccines. The study included four groups of local breed goats, where the first received modified live PPR virus vaccine; the second received inactivated bivalent FMD virus vaccine; and the third group received simultaneous vaccination with PPR and FMD vaccines. A separate group was left non-vaccinated and served as control. All animals remained clinically normal throughout the whole...
Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin2
...time, the 28th day after immunization for work, serum samples were taken for passive hemagglutination test (PHA); Immunoglobulin G(IgG) detection using a chemical immunosorbent assay test. the outcomes of PHA test revealed that Folcord inhibited humoral immune response, with a considerable rise in antibody titer in the third and second groups, but decreased serum antibody levels in the first group. Furthermore, the (IgG) titer in serum is measured using the En...
Sijie Jian1,2,3,4, Wei Sun1,3, Jia Chao1,2, Na Rong1,4, Xiang Liu1,2,3,4* and Chen Chen1,2,3,4*
... P. fluorescens. Passive immunization of Carassius auratus with Slp mouse serum and challenging with bacteria showed a passive protection rate of Slp serum against A. hydrophila infection of 42.5 % (p < 0.05) and a passive cross-protection rate against P. fluorescens of 18.6 %; the immune-related factors of LZM, AKP and ACP and the leukocyte phagocytosis of phagocytic percentage (PP) and phagocytic index (PI) increased (p < 0.05); the inflammation-relat...
Muhammad Wasif Gulzar*, Riffat Maqsood, Muhammad Zain, Muhammad Suleman, Tayyab Ur Rehman, Sana Asif, Abdul Wadood and Jawad Hussain
...rehensive information on immunization, treatment techniques, pathophysiology, epidemiology, transmission, and relevant preventive and control measures....
Liyun Chang1*, Yumei Cai1, Chenghui Li1 and Jianhua Qin2*
... Eimeria acervulina oral immunization group, the naked plasmid DNA vaccine pcDNA3-3-1E of Eimeria acervulina oral immunization group and control group, with 30 chickens in each group. The chickens were immunized with a dose of 50μg/chicken, respectively, and strengthened immunity one week later. At 7th, 14th, 21st, 28th, 35th day post-injection, respectively, after extracting the total DNA from fresh tissues of immunized ...
Sundus Oun Ali Al-Zaini1, Mohanad A. Al-Bayati3*, Khazaal Abbas Khazaal2, Salma Talib Salih1
...op two oral vaccines for immunization against Koi Herpesvirus (KHV) and Aeromonas hydrophila antigen in common carp, Cyprinus carpio, using formalin-inactivated versions of both virus and bacteria (mean body weight of 108.2±1.2g). Vaccines that have been prepared and loaded into Nano liposome blank that have been formulated according to the thin film method (Bangham). The experimental was designed into four fish groups for vaccinination. The Formulated ...
Yingying Wang1, Sufen Zhao2* and Qunxiu Liu3*
...se two diseases, poultry immunization procedures have been rigorously standardized. Wild birds in Shanghai Zoo are currently immunized against both diseases with commercial vaccines for birds, but their immune status and the immune effect are not very clear. In order to determine the protective effect of the current AI (H9 subtype) and ND vaccines on wild birds in Shanghai Zoo and to explore a more reasonable and effective immunization...
Basirat Opemipo Amoo-Adeboye1*, Kazeem Olanrewaju Amoo2, Saheed Adeyinka Odediji3, James Akinwunmi Ogunniran1, Adenike Titilayo Olayinka1 and Oluyinka Oladele Opaleye1
Dipa Rani Pal*, Injamamul Haque Rasel, Tarun Chandra Mondal, Mohammed Lalmoddin Mollah and Shampa Rani
...n, age of birds, lack of immunization plans, biosecurity measure and inadequate management practices....
Jaafar Haider Abd Alrudah1, Nada Fadhil Abbas2*, Farah Ali Hadi1, Haider Tuma Kaab3
...even days after the last immunization. The results showed that the antigen prepared from local isolate induced an immune response that protects or reduces the severity of infection with Candida albicans in birds. This study was based on the previous research to produce an antigen from whole-cell fraction (WCF) antigen in mice. The estimation of humoral immune responses and IgG showed that the levels of the antibodies were higher in the immunized grou...
Evaluation of Oil Vaccines of Avian Influenza H5 Virus in Layer by Enzyme-Linked Immunosorbent Assay
Hasan Muwafaq Ali*, Raed Hussain Salih Rabee
...mune system, large-scale immunization campaigns may utilize live-attenuated vaccines. Aim of study: This research aimed to assessing the effectiveness of oil vaccines against the H5 virus of avian influenza using an enzyme-linked immunosorbent test in layers. After inoculation with the killed oil vaccine against influenza were tested for H5 immunity in Iraqi Layers hens (LOHMAN Brown). Four treatment groups of 192 chicks were immunized at 1 to 210 days. AI tit...
Qayssar Obaid Muhammed, Raed Hussain Salih Rabee*
...s to a novel strategy of immunization employing an inactivated oil-emulsion of Newcastle disease virus vaccine administered through fine spray. The study also examined the long-lasting nature of passive antibodies in layer hens and evaluated their effectiveness in providing protection against different strains of viruses. There were 200 Lohmann Brown chicks, all 1 day old, which were randomly divided into four groups, each containing 50 chicks. Groups 1, 2, an...
