Muhammad Waheed Mushtaq1,*, Jamil Anwar2, Omara Naeem3,Farah Kanwal1 and Waheed-uz-Zaman1
...>.
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Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
...up>C and liquid nitrogen freezing at -196°C) are used by different laboratories. In this study, the storage stability of five bacteriophages, JHP, RLP, RSP, SaPL, and IttPL, at distinct temperatures for complete one year is described. Our results indicated that storage of bacteriophages at 4oC was most appropriate, followed by storage at -20oC and -80oC as no drop in viral titer was witnesses in bacteriophage samples stored...
Nizam Uddin Jamali1, Asmatullah Kaka1,*, Pershotam Khatri1, Moolchand Malhi2, Muhammad Naeem3, Akeel Ahmed Memon1, Rameez Raja Kaleri4, Habibullah Janyaro5 and Dildar Hussain Kalhoro6
Muhammad Waheed Mushtaq1,*, Muhammad Waqar Younas2, Jamil Anwar2, Syed Mustansar Abbas3 and Shahid Bashir3
...oncept of unidirectional freezing. To separate the enzymes from their aqueous system, directional freezing was applied horizontally through synthetic enzyme contaminated water samples, called radial freezing. After complete freezing, the samples were collected from various sections of the frozen mass and concentration of enzymes was determined. It was fo...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
Saeed Murtaza1, Abdul Sattar1*, Nasim Ahmad1, Muhammad Ijaz2, Maqsood Akhtar3 and Muhammad Shahzad4
...t fat, protein, lactose, freezing point, SNF and solids were significantly higher (P<0.05) in group-2 and group-3 as compared to group-1 except density and pH which remained non-significant (P>0.05) in all groups. On the basis of result, it may be concluded that oxytocin had no effect on uterine involution and progesterone; however, it had some role to affect the normal composition of milk in postpartum involution interval.
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Amit Sharma1* and Pankaj Sood2
...ate, equilibration time, freezing and thawing rate. All these factors should be given due credit for achieving better post-thaw post thaw quality of semen to be utilized in different advanced reproductive techniques. ...
Savas Atasever*, Ali Vaiz Garipoglu and Huseyin Erdem
...ctose (L)), density (D), freezing point (FP), somatic cell count (SCC) and daily milk yield (DMY) according to the different feeding applications (grazing (G), silage usage (S), compound feed usage (C), number of milking cow (NMC), calf suckling period (CSP)). Average F (3.144±1.931%), P (3.022±0.448%), L (4.475±0.669%) and logSCC (5.386±0.529) values were within acceptable ranges. The present investigation revealed that feeding pra...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...of spermatozoa following freezing and thawing. It is suggested that the addition of 1.5% green tea extract (4 g in 200 ml methanol) can be safely used for enhanced effectiveness of fertility after thawing.
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Amir Z. Allam, Hayam A. Mansour, Nermeen M.L. Malak, Heba H.S. Abdel-Naeem*
...on and during the entire freezing storage. Additionally, the highest reduction rate in APC, psychrotrophic, and Enterobacteriaceae count was observed in samples treated with mixtures of three oleoresins (T-CL-O) as compared with other groups. In conclusion, the food industry could use a mixture of these oleoresins with a low concentration as a natural source of antibacterial during the processing of chicken meat products to improve their bacterial quality and ...
Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
... cooling, equilibration, freezing and thawing stages. They damage functional and morphological characteristics of sperms. Beside these exogenous stresses there is an oxidative stress which damages the spermatozoa endogenously. These stresses can be controlled with the inclusion of antioxidants in semen extender at the time of cryopreservation. In the current study, semen from four (n=4) healthy Nili Ravi buffalo bulls was collected through artificial vagina an...
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
...am semen extender during freezing steps. Semen was collected by an artificial vagina from five adult Rahmani rams twice-weekly for five weeks. Semen was pooled, split into five replicates, and prepared to be frozen. In 1st replicate, semen was extended in Tris egg yolk (TEY) is contained synthetic sources of energy (fructose), antibiotics (penicillin and streptomycin), and antioxidants (vitamin E), which served as control extender (E0). However, 2nd, 3rd, 4tha...
Entesar Z. Eliraqy1*, Basant M. Shafik2, Yasser S. Hussein1, Abdelwahab A. Alsenosy3, Emad A. Abd allah1, Mohamed F. Saad1
...meters after cooling and freezing process. Data obtained showed that, progressive motility, live spermatozoa and acrosome integrity % were higher (P < 0.05) in G1, G2 and G3 with reduced mortality percentage than control group after cooling process. In addition, a significantly increased (P < 0.05) of progressive motility, acrosome integrity and alive spermatozoa percentage in groups treated with different concentrations of garlic extract than control gr...
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...e sperm motility, before freezing, post-thawing, and during recovery across age (p > 0.05). Semen volume, total sperm, total motile sperm, and frozen semen production increased in bulls from 6 to 7 years of age and then gradually reduced from 7 to 10 years of age. It could be concluded that the optimum semen productivity of Aceh cattle is when they are 7 years old.
Keywords | Artificial insemination, Frozen semen production, Indonesian local cattle,...
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
Yusmarini Yusuf*, Usman Pato*, Shanti Fitriani, Emma Riftyan, Evy Rossi, Diky Arma Fauzi, Ghina Ismadiah, Miftahul Hidayah, Windy Sabiliani
...l samples were stored at freezing temperatures for 30 days. Data were analyzed statistically using analysis of variance, and differences between treatments were analyzed using DNMRT at the 5% level. The results showed that the application of various preservatives might impact the quality of fishballs during the first 30 days of frozen storage. Even though the chemical and sensory parameters of fish meatballs without preservatives that were kept at
I Nyoman Sulabda1, Anak Agung Gde Oka Dharmayudha2, I Wayan Nico Fajar Gunawan3, Dwi Fortuna Hashilonda4, Luh Gde Setyawati5, I Ketut Puja6*
...it can be concluded that freezing the semen of Kintamani dogs could fertilize female dog eggs.
Keywords | Kintamani dogs, fertilization, Insemination, Intravaginal, Pregnancy
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Tarique Ahmed Khokhar1*, Atta Hussain Shah1, Muneer Ahmed Jamali1 and Asghar Ali Kamboh2
... the effect of chilling, freezing and repeated-thaw cycles on chemical quality of various meats. The influence of different time was observed on chemical characteristics of chilled, frozen and thawed meat against fresh meat. Proximate composition such moisture, protein, fat, ash and glycogen decreased with increasing storage period of chilled, frozen and thawed buffen, chevon and chicken meat samples. Nutritive values of chilled, frozen and thawed buffen sampl...
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
..., namely motility before freezing (70.86%) and post thawing (53.00%); viability before freezing (76.00%) and post thawing (60. 14%); plasma membrane integrity before freezing (71.29%) and post thawing (59.86%); and also showed the lowest abnormality level before freezing and post-thawing, (17.29%) and (24.14%), respectively.
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
...s during the cooling and freezing process, and especially significant changes are seen after freezing than after cooling. This research analyzed the effect of genistein supplementation in the basic diluent of tris-aminomethane on the quality of spermatozoa. Bulls aged 3–4 years used in this research. This research used an experimental method to examine the effect of genistein levels in the diluent on the characteristic...
Luigi Liotta1, Vincenzo Lopreiato1, Fariborz Asroosh2 and Alireza Seidavi2*
...ng colour, odour, taste, freezing point, dry matter free of fat, acidity (lactic acid), density (in 15 °C), pH, fat and protein were determined according to the standard methods. Macro and micro elements of milk were analyzed using inductively coupled plasma mass spectrometry. Data were subjected to the two-way ANOVA analysis to determine the significant differences between species/breeds. The acidity, density and fat in Talesh sheep’s milk was highe...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...f semen was achieved pre-freezing in equilibrated semen and post-thawing in semen thawed in a water bath at 37oC for 30 seconds. Results showed that PEE2 showed the highest (P < 0.05) positive impact on improving progressive motility, livability, abnormality, and acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) viable sperm percentage, and level of total anti...
Khanitta Pengmeesri1,2* Thassawan Somchan1 , Doungnapa Promket1,3
...splays disparities after freezing, favoring purebreds for quality. Crossbred natives reached market weight faster than purebreds, but after freezing, purebreds exhibited a quality advantage. ...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...e semen was assessed pre-freezing and post-thaw with parameters such as viability, motility, abnormality, and plasma membrane integrity. The findings revealed that the inclusion of 1% and 2% lycopene in the diluent before freezing exhibited significantly higher (P < 0.05) spermatozoa motility. Moreover, the viability and motility of post-thawed semen significantly improved (P < 0.05) at lycopene doses of 1% and 2%. The...
Kenyum Lollen1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
...t dilution ratio using 3 freezing protocols with two step cooling profile in programmable freezer. The spermatological properties of milt obtained from non-induced fishes were superior which was further used for short term preservation. When experimented with dilution ratio, the highest motility duration of 74.3±2.16 s was obtained at 1:10 dilution ratio. The best results on motility duration (62.28 ± 2.12 s), fertilizing ability (77.3±1.6...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
Aulia Puspita Anugra Yekti, Ani Atul Arif, Putri Utami, Habib Asshidiq Syah, Anggita Dian Pramudhita, Rr. Ani Rizqianti, Trinil Susilawati*
...yolk in the sexing semen freezing process.
Keywords | Percoll density gradient centrifugation, Proportion, Quality, Sexing sperm, Tris aminomethane+20% egg yolk
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Siew Ing Nguang, Nur Syafiqah Binti Zainal, Ahmad Mukhlis Bin Hafas, Hou Chew Ha, Connie Fay Komilus, and Asmad Kari*
...genetic potential during freezing and thawing. Each fish species requires a specific extender because of diverse sperm characteristics. Tilapia (Oreochromis sp.) popular in Malaysia for its hardiness and rapid growth is an excellent choice for aquaculture. Aloe vera (Aloe barbadensis) (Av) shows promise in spermatogenesis, but its use as a cryoprotectant in semen extenders is still underexplored. This study investigates the effects of antibiotic-...
Samah Abou Asa1, Omnia Tag2 and Walid Elmonir2*
...ming fresh without prior freezing (92.7%), salting fish for less than 10 days (50%), and cooking fish for less than 10 min (2.3%). Health education for residents and infection control in fish are mandate preventive measures in the study area....
Lalu Ahmad Zaenuri*, Rodiah Rodiah
...ture, refrigeration, and freezing, on semen preservation. This would not only be beneficial for AI programs, but also contribute to the existing literature on semen preservation extenders.
Keywords | Tris, Albumin, Fig fruit filtrate, Spermatozoa, Goat
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Haiyan Wen1*, Kai Liao1, Zhengrong Yuan2 and Ning Zhou2
...ffect of different quick-freezing methods and different storage temperatures on the content of cryoprecipitated blood coagulation factors in fresh plasma. The convenient sampling method was used to select 200 ml of whole blood from 60 unpaid blood donors who entered the blood station from January 2022 to October 2022. The acquisition time is controlled within 10 min. The plasma samples were divided into six groups (A, B, C, D, E, F) by using a random number ta...
