Aradhana Chopra1, Ravi Shukla2 and Tarun Kumar Sharma2,3*
...o in other areas such as diagnostics, biomarker discovery, drug delivery, bio-imaging and molecular therapy. In this review we have summarized recent advancements and applications of aptamers in biology....
Nicola J. Stonehouse
...s research as well as in diagnostics and therapeutics....
Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2
E-mail | Tomas.Jinnerot@sva.se
...rters. Fast and accurate diagnostics enables the selection of proper management. Our objective was to develop a sensitive and specific real-time PCR for detection of B. bronchiseptica in samples from dogs with respiratory signs. A genome comparison program was used to select a suitable target DNA sequence. A PCR targeting the bfrZ gene was developed which showed no cross-reactivity in silico or when tested with a panel of ...
Mohammed M. Islam1, 3 *, Damian F. J. Purcell2 and George Kannourakis1,3
... been proposed for viral diagnostics with variable outcomes and successes. In this review, we discuss a novel approach to identify the least transcripts of viral genomes using suppression subtractive hybridization that can be adapted to unanimously identify viruses of diverse genetic backgrounds. ...
Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*
...elopment of vaccines and diagnostics for differentiating infected and vaccinated animals. With the advent of recombinant DNA technology, recombinant protein based vaccines and/or diagnostics are being tested in various heterologous systems across the globe for development of vaccines and/or diagnostic antigens. The recombinant viral proteins, virus like particle based vaccines, bivalent/multivalent vaccines, recombinant vira...
Neha Singh and Anjana Pandey
...y useful in the field of diagnostics and targeted therapy and the aptamers generated can be utilized to differentiate cancerous cells from normal cells or even different types of cancerous cells, detect live pathogenic organisms, and to discover novel disease biomarkers to facilitate early detection of the disease and for therapeutic purposes....
Tarun Kumar Sharma, John G. Bruno and William C. Cho
...te space in research and diagnostics markets. However, translation of aptamer in several applied disciplines is still lagging behind as compared to antibodies. In this article, we discuss the translational aspects of aptamers, current potential of aptamers and strategies to create the “bench-to-bedside” translation of aptamer technology....
John G. Bruno
...improved rickettsial serodiagnostics and aptamer-based vaccines....
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...ainst phytonematodes and diagnostics of economically important plant parasitic nematodes. Specific isozymes have been identified for differentiating the important root knot nematodes species of India belonging to the genus Meloiodogyne. Further, molecular markers like RAPDs, AFLP, SSRs and PCR–RFLP of rDNA have been employed to study the molecular genetic diversity in major root knot and cyst nematode species of India to assist the breeding program for d...
G. Venkatesan* and Amit Kumar
...n using latest molecular diagnostics and effective vaccine along with anti-viral therapeutics. This short commentary deals with current knowledge and opportunities on ORFV and future challenges as control perspectives in India. ...
Zafar Iqbal1, Muhammad Farooq Nasir1, Imran Bodlah1*, Rahmatullah Qureshi2, Ayesha Aihetasham3
Notes on three morphs of Bulaea lichatschovii (Hummel) (Coleoptera: Coccinellidae) from Northern Pakistan
...umented. Notes on
diagnostics of the species are given with illustrations and new distributional
records....
diagnostics of the species are given with illustrations and new distributional
records....
Anand Kushwaha, Amit Kumar, Aparna Madhavan, Durga Goswami, Golmei Poulinlu and Gnanavel Venkatesan*
...rictions. Though several diagnostics including molecular tools are available, recombinant protein based diagnostic assays namely ELISA is safer and robust to handle large sample size and also to minimize labor/time. However, the genus Capripoxvirus encodes putative 147 proteins in their genome, among which some of them are reported as potential immunogenic candidate genes. Selection and use of such candidate immunogenic proteins from an array of genes located ...
Hasaan Aziz*, Fariha Altaaf, Attia Bashir, Abdul Basit, Syed Anam Masood Gardazi and Farah Haider
...an to devise appropriate diagnostics and control strategies is yet to be known. Despite the use of modified live vaccine to dogs, occurrence of disease is not uncommon. Here, we sequenced and phylogenetically analysed the complete hemagglutinin gene of CDV from a male dog (14 months old) exhibiting clinically suggestive symptoms. With a characteristic amino acid residue for dog at Y549, sequence analysis showed geographically distinct pattern clustering the st...
Syed Maaz Nadeem1,*, Muti ur Rehman Khan1, Asim Aslam1, Ali Ahmad Sheikh2, Arfan Ahmad2, Syed Nadeem Mansoor3 and Muhammad Anees4
...ed for efficient disease diagnostics in resource-limited setting.
...
Amjed Ali1, Muhammad Tayyab1,*, Sehrish Firyal1, Abu Saeed Hashmi2, Asif Nadeem3, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1
...;-lactamase for clinical diagnostics in Pakistan. The current work deals with the optimization of conditions for higher level production of recombinant β-lactamase from Bacillus subtilis R5. Supplementation of LB medium with various carbon sources including wheat bran, rice bran and molasses could increase the enzyme production from 25 to 43.7, 44.3 and 49 µmole/min whereas the nitrogen sources including tryptone, peptone and yeast extract co...
Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3
...ods for such pathologies diagnostics, along with haematological analysis, include the replicative and expressive activity of FeLV evaluation in the infected organism cells, innate immune factors for antivirals evaluation, interferon system (IFN) in particular, molecular guard factors for cell genome integrity evaluation (p53, etc.). Methods. In domestic cats with a previously established diagnosis of «FeLV-infection and chronic leukaemia» (n = 10) ...
Ghassan Zahid1, 2*, Sara Iftikhar3, Muhammad Umer Farooq4 and Shakeel Ahmed Soomro5
...ybrid detection, disease diagnostics and sex differentiation by administering the powerful diagnostic polymorphism detection tools at specific loci and the genome level. This review focuses on exploring the application, recent advancements and future implications of DNA-based molecular markers with the context of their role in the genetic improvement of fruit germplasm that is widely grown in Pakistan like citrus, apples, mangoes, guavas, bananas, date palms a...
Amjed Ali1, Muhammad Tayyab1*, Abu Saeed Hashmi2, Asif Nadeem3, Shumaila Hanif4, Sehrish Firyal1, Shagufta Saeed1, Ali Raza Awan1 and Muhammad Wasim1
...n as positive control in diagnostics and in antibiotic susceptibility testing experiments....
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
... detection, ELISA, Rapid diagnostics, dairy industry, Human health hazards, Granulomatous diseases
... 
JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5
...Citrus canker, Molecular diagnostics
...
Kouengoua Kouengoua Armelle Prudence1*, Yessinou Roland2, Nankam Chimi Roland1, Fotsac Dzousse Muller1, Kouam Alain1, Djuikwo Felicite1, Facho Balaam1, Awah Ndukum Julius3, Farougou Souaibou2
...ausal agents, methods of diagnostics, risk factors. After several screenings, six articles were considered for this systematic review. East and Southern Africa countries carry on most researches on bovine tuberculosis in wildlife. Available studies assessed the status of wild mammal, carnivore. Further researches to improve the knowledge on the status of bovine tuberculosis in wildlife in Africa are still needed in order to allow proper disease monitoring in w...
Gunel Arzuman Ramazanova1, Gulnara Fakhreddin Abbasova2*, Khalsa Ibrahim Nasibova3 and Sait Engindeniz4
Muhammad Sajid1*, Muhammad Hamid2, Usama Ayyub2 and Muhammad Azam2
... diagnostic testing, and diagnostics, and antiviral treatment of CCHF, we provide a critical reviewed of the literature. The chemical interactions and life cycle of viruses are intricate and are poorly understood. Despite our limited understanding of pathogenesis and immunobiology, multiple routes contribute to the viral entry, replication, and clinical damage. A few autopsy reports have highlighted mention hemorrhages and extravasation involving many body org...
