Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...maximum primary calli on callus induction medium containing both 0.5mgL-1 2, 4-D and 1 mgL-1 picloram. Eighty percent of embryogenic calli were obtained on MS medium containing only single auxin while combination of auxin and cytokine in MS and Chu’s N6 media produced 53.33 and 35.56% embryogenic calli, respectively. Inclusion of 5 mgL-1 silver nitrate in embryogenic medium enhanced the production of type II embryogenic calli by 54.90% compared to contro...
Muhammad Akram and Faheem Aftab
THIDIAZURON INDUCES IN VITRO BUD BREAK AND SHOOT DEVELOPMENT FROM NODAL EXPLANTS OF ORTHOTROPIC SHOOTS OF MAIDENHAIR TREE (GINKGO BILOBA L.)
... the growth of shoot and callus formation was observed. Shoots were cut to prepare nodal explants (5-10 mm long), surface sterilized and inoculated on MS (Murashig and Skoog, 1962) medium supplemented with different concentration (0.001, 0.005, 0.01, 0.05, 0.1, 0.5, 1,2,3,4 or 5uM) of TDZ for 24 days. Highest bud break (100%) was obtained at 0.005 uM TDZ after 14 days of initial culture. The same cultures were further maintained and subsequently obtained with ...
Rizwan Ullah Shah* and Iqbal Munir
...ledon and hypocotyl) for callus induction followed by shoot and root regeneration of Brassica carinata. Seeds were sterilized and germinated, of which one week cotyledon and hypocotyl were used for callus formation and shoot regeneration at five different level of 1-naphthaleneacetic acid NAA (0.05, 0.1, 0.5, 1.0 and 1.5 mg/l) and 6-benzyl amino purine BAP (0.1, 0.5, 0.7, 1.0 and 1.5 mg/l), and root regeneration at four leve...
Betül Ayça Dönmez, Sarbesh Das Dangol and Allah Bakhsh*
...d with AGL1. The highest callus formation for both cv. Desiree and S. chacoense M6 was obtained on cv. Desiree leaf explants, transformed via the Agrobacterium strain GV2260. The lowest callus formation, for both S. chacoense M6 and cv. Desiree, were obtained from internode explants. About 33% calli induction was achieved from transformation mediated by AGL1 strain in cv. Desiree, and about 23 % from LBA4404 strain in S. cha...
Zulekha Zameer, Samreen Mohsin, Ammarah Hasnain, Asma Maqbool* and Kauser Abdulla Malik
Influence of Explant Sources on in vitro Callogenesis and Regeneration in Maize (Zea mays L.)
... for its ability to form callus and to regenerate subsequently. For callogenesis from different parts of germinated seedlings, maximum frequency was shown by bulged inter node (45.60 %) along with the regeneration efficiency of 46.23 %. In case of split seed technique, the efficiency for callogenesis was 82% with the regeneration efficiency of 65.46%. For mature embryos the efficiency for callogenesis was 61% while for regeneration it was 78.06%. The results r...
Fahrauk Faramayuda1,2*, Totik Sri Mariani3, Elfahmi1,4 and Sukrasno1
...ve plant tissue culture (callus induction). This research aim is to identify secondary metabolite in callus using thin layer chromatography (TLC). The profiling of ethanol extracts of callus of two varieties O. aristatus were carried out using solvent system toluene-ethyl acetate- formic acid-water (3:3:1:0.2). The results of monitoring TLC suggest that callu...
Muhammad Rafique1, Muhammad Azhar Iqbal2, Inam Ul Haq1*, Muhammad Ramzan Anser2, Humara Umar2 and Muhammad Ashraf Sumrah2
...d cutting percentage and callus percentage were collected under four different treatments. Cutting under the only green net (5944 lux), white net (11428 lux) only upper side white net (8484 lux) and both green and white net (5538). The results were found significant regarding the interaction between varieties and treatments; however, non-significant variation in varieties was found regarding the number of roots, days to root, and days to shift. The important o...
Thomby Paul, Sreekanta Biswas, Sabiha Zarin Tasnim Bristi, Debashish Sarker, Saroj Kumar Yadav and Bhajan Chandra Das*
...igration of implants but callus formation was observed between the fracture ends of olecranon in order that the implants were removed. On 45th day of clinical observation, the dog showed good weight-bearing in right forelimb and walked normally....
Mazhar Ullah and Mohammad Sayyar Khan*
...mized to produce maximum callus and regenerated plantlets. In this research, different auxins [Dichlorophenoxy Acetic acid (2,4-D), Nephthalene Acetic Acid (NAA)], cytokinin [Benzyl Amino Purine (BAP)] either alone or in combination with each other and carbon sources [sucrose, glucose and fructose] with varying concentrations (2, 4 and 6%) were evaluated for their effect on the tissue culture of sugarcane variety-CP 77/400. For callus<...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
...hest growth response for callusing 78.3% was observed in BAP (0.5mg/L) in combination with Kinetin (0.25mg/L) +NAA (2 mg/L) followed by 65% at 2 mg/L of 2,4-D. Maximum percentage of shooting (81.67%) with the maximum mean value for number of shoots (7.56) and shoot length (7.07) occurred when the fresh shoot tip of C. tuberculata were planted on to MS salts containing 2 mg/L of BAP, 0.5 mg/L of NAA and 0.25 mg/L of Kin. The new shoots were then inoculated on 1...
Muhammad Shafiq1, Tehseen Ashraf2*, Sehrish Mushtaq1, Naveeda Anjum3, Muhammad Asim4, Muhammad Aqeel Feroze3, Malik Abdul Rehman4 and Marja Aziz3
...pes tested. Maximum mean callus induction rate of hypocotyl was 76.6% in SP followed by TP (73.3%); Sanam (57.3%); Loungi (43.3%) respectively. Whereas in case of cotyledon maximum inductions rate was found in Loungi (62.0%) followed by SP (60); Sanam (57.3); TP (46) individually. According to the observations maximum plant regeneration was found in genotype SP (9%), followed by Loungi (1.8%); Sanam (1.5%) but no plant regeneration was found in TP. A. tumefaci...
Inam Ul Haq1*, Humara Umar1, Farah Umar2, Naeem Akhtar3 and Muhammad Jan4
...tment also showed higher callusing 80.5%, sprouting 83.3% and survival rate 51.1% as compared to all other treatments. Among other treatments T5 (IBA+PB) and T4 (IBA+UP) are ranked second and third respectively in term of higher rooting ability. The minimum rooting was observed in T7 (UP+PB). The present study demonstrated the effect of combined treatment of IBA+UP+PB on stimulation of rooting and survival of cuttings compared with a treatment of IBA (T1) bein...
Muhammad Adeel Qureshi1*, Raza Ahmad2, Bilal Ahmed1, Tanzim Ullah Khan1 and Muhammad Yasin3
...find a best protocol for callus induction and plant regeneration of potato (Solanum tuberosum L.). The experiment was carried out in complete randomized design (CRD). Leaves and internodes of potato cv. Desiree were used as explant for callus induction and plant regeneration. Two types of callus induction media (CIM) were used, i.e. CIM1 and CIM2 with concentrations of 2, 4-D 2mg/lit and 3...
Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5
...(TDZ). As a result, 100% callus induction was achieved on the medium containing 1.0 µM TDZ. Notably, calli grown on 1.0 µM TDZ exhibited greater potential for shoot regeneration and proliferation in the growth regulator-free MS liquid medium (95.25%) as compared to the solid medium (89.00%). Shoot cultures remained viable for up to 15 days without refreshing the medium and could be sustained for 12 months when sub-cultured every 15 days. Invigorati...
Najamuddin Solangi1*, Mushtaque Ahmed Jatoi1, Adel Ahmed Abul-Soad2, Abdul Aziz Mirani1, Muhammad Aslam Solangi3 and Ghulam Sarwar Markhand1
...). Significantly highest callus induced in floral buds in cvs. Begum Jungi (87.3%) and Ajwa (84.3%) on medium comprising of 2,4-D (2.0 mg L-1), 2iP (0.5 mg L-1). Medium comprising of 0.05 mg L-1 2,4-D, 2.0 mg L-1 2iP, 3 g L-1 activated charcoal induced significantly highest somatic embryos in cvs. Begum Jungi (83.3%) and Ajwa (82.6%). Somatic embryos induced in calli after nine months of initial culture were categorized into repeated and non-repeated. Medium c...
Tanvir Hussain
...sponse to all media. For callus initiation, same ex-plants and media (alone and in combination with callus initiators) were tried but callus formation was not observed. Aseptically grown young plants were then transferred to hydroponic conditions for maturity. For this purpose MS (Full-strength, 1/2, 1/4), B.N.M and Hooglands solution were used. It was researched out that 1/4 MS (pH 5.8) a...
Rehana Perveen, Mehmood Akram and Ihsan Ilahi
...d differentiation of the callus. At NAA 1 mg/1,
BAP 0.5 mg/1 NAA 0.5 mg/1 roots developed at 0.5mg/1 of
BAP & NAA 0.5+1.0 mg/1 of BAP shoot buds also developed.
Callus pieces cultured on MS medium supplemented with
different combinations of NAA &BAP showed progressive
anatomical changes accompanying differentiation of roots
and shoot buds....
Q. N. Javeed, Rehana Perveen, Imtiazul Haq and Ihsan Elahi
...
...
Krishna Adhikari1, Suman Poudel1 and Manoj K. Shah2
... and absence of bridging callus on the alignment, with the dog being able to walk normally and bear proper weight on the affected limb, respectively. The IM Steinmann pin was removed from the affected limb in the 20th week following pin migration....
