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Three Bacteriophages SA, SA2 and SNAF can Control Growth of Milk Isolated Staphylococcal Species

Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*

...astitis. In ongoing era, bacteriophages are an alternate possible effective remedy. Therefore, in this study the infection ability of three lytic phages SA, SANF and SA2 was determined against ten isolates of Staphylococci and one Micrococcus, isolated from five raw milk samples. Their morphological, biochemical characterization and 16S rRNA sequencing indicated that two strains were S. aureus, while all others were different coagulase negative Staphylococci. ...

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
... amplification using the bacteriophage 29 DNApolymerase allows for a reliable diagnosis of geminiviruses and presumably all viruses with small single-stranded circular DNA genomes. The results show the efficiency of this technique in characterizing viral DNA components of several geminiviruses from experimental and natural host plant sources. Nucleotide sequence data offers identification of viral molecule to strain/species level for accurate diagnosis....
Storage of Bacteriophages at 4°C Leads to no Loss in Their Titer after One Year
Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
.../div>...
Stability of Salmonella typhimurium Bacteriophage to Some Physical and Chemical Factors
Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1
...8 kbp.
...
Abundance and Variation of Virulence of Rhizobiophages Isolated from the Rhizosphere of Some Legume Plants in Egypt

Ramy A. Qabel1; Tarek F. El-Arabi2; Adel A. Shoukry1; Hassan H. Elsebaay1; Abdel-Aziz F. El-Hamahmy1

...d affect the rhizobia is bacteriophage infection. In this context, this study aimed to investigate the abundance of rhizobiophages in Egypt. Rhizobiophages were isolated from the rhizosphere of different legume plants. While no temperate phages were detected, two groups of phages were determined based on their virulence. Highly virulent phages designated as group V showed clear plaques and were able to infect many of Rhizobium species and strains. The other gr...
Isolation of Pseudomonas aeruginosa Phages from Wastewaters

Stephen Chijioke Emencheta1, Chinelo Charity Eze1, Anthony Amaechi Attama2, Damian Ejike Agbo1 and Ebele Benedette Onuigbo1*

...st the lytic action of a bacteriophage specific to Pseudomonas sp. Both Pseudomonas spp. and bacteriophages were isolated from residential wastewaters. The isolated Pseudomonas spp. were confirmed through biochemical tests. Antibiogram was done with nine (9) different antibiotics, including;aztreonam (ATM), chloramphenicol (CPL), gentamicin (GTN), tetracycline (TTE), sulphamethoxazole/trimethoprim (SXT), amoxicillin/clavulan...
Isolation of Escherichia Coli Phages from Waste Waters

Stephen Chijioke Emencheta1, Anthony Amaechi Attama2, Ezinwanne Nneoma Ezeibe1, Adaora Angela Agubata1 and Ebele Benedette Onuigbo1*

...st the lytic action of a bacteriophage specific to Escherichia coli. Both Escherichia coli and bacteriophages were isolated from farmlands and residential wastewaters. The isolated Escherichia coli were confirmed through phenotypic and biochemical tests. Antimicrobial susceptibility test to nine antibiotics was determined. Multi-antibiotic resistance index (MARI) was assessed. Spot assay was done to determine lytic action of...
Application of Phages to Control Escherichia coli Infections in Native Noi Chickens
Luu Huynh Anh1, Huynh Tan Loc2, Nguyen Hong Xuan3, Le Minh Thanh1, Trinh Thi Hong Mo4, Ly Thi Thu Lan5, Nguyen Trong Ngu1*
Effect-of-Bacteriophages-on-Intestinal-Colonization-of-Escherichia-coli-Cecal-Microbiota-Composition-Intestinal-Morphology-and-Growth-Performance-in-Nursery-Pigs-from-Commercial-Pig-Farms

Pawinee Kingkan1, Thanathip Supcharoenkul1, Chaowit Rakangthong1, Chaiyapoom Bunchasak1, Komwit Surachat2,3, Wiriya Loongyai1* 

...termine the effects of a bacteriophage cocktail on growth performance, intestinal morphology, E coli detection, cecal bacterial composition, and the incidence of diarrhea in nursery pigs for 6 weeks. A total of 800 pigs (Large White × Landrace × Duroc) were randomly allocated to two treatments: a basal diet supplemented with a mixture of amoxicillin and colistin (Amox-Co) and a basal diet supplemented with amoxicillin and 1 g/kg of a
Effects of Bacteriophage Cocktail to Prevent Salmonella enteritidis in Native Broiler Chickens

Nguyen Thi Anh Thu1*, Ly Thi Thu Lan1, Nguyen Thuy Linh1, Chau Cong Dang1, Nguyen Hoang Phuc1, Tran Dinh Tuan1, Nguyen Trong Ngu2 

...valuate the effects of a bacteriophage cocktail (BC) against Salmonella enteritidis (SE) in native broiler chickens. Totally, 320 native chicks were divided into two experiments using a completely randomized design consisting of 4 treatments and 4 replicates of 10 chicks per experiment. In both experiments, BC was used at a dose of 109 PFU/mL, and SE was used at 108,2 CFU/mL. In the protective experiment, 1-day-old chicks were arranged in CD) control diet; BC+...
Cocktail Phage Therapy for Bacteria Contaminating Meat in Egypt
Amany M. Reyad1*, Aya Maher Rabie1, Reda Mohamed Taha1 and Khalid El-Dougdoug2
Physiological Profiling of Lytic Bacteriophages and their Efficiency against Salmonella Enteritidis on Chicken Breast Cuts

Aleena Kokab1, Ali Ahmad Sheikh1*, Masood Rabbani1, Wasim Shehzad2, Muhammad Ilyas Riaz1, Sohail Raza1 and Rida Haroon Durrani1

...-food sector. The use of bacteriophages, as a potential replacement, for antibiotics and as food antimicrobial agents to potentially target foodborne pathogens is gaining augmented attention. This study’s goal was to isolate and physiologically characterize lytic bacteriophages against Enteritidis, and to ascertain their efficacy in modulating bacterial population on chicken breast cuts in the form of a phage cocktail....
Virological Evaluation of Drinking Water in Egypt

Azzami, Mohamed 1.; Hakiml , Aya Salah El-Din; Othman2, Badawi A. and 2 El-Dougoug, Khaled A.

...ail with full heads, the bacteriophage resembles those belong to the Myoviridae family. Enteroviruses were detected using RT-PCR and real-time-RT-PCR in inlet water of El-Bagour and Shibin ElKom stations in summer season, while H5N1 was not detected in all sites throughout four seasons. The viruses obtained by ultrafiltration process were negatively stained with 2% phosphotungestic acid. Electron microscopy revealed that human virus particles were of 24-30 nm ...
Isolation and Characterization of a Pseudomonas aeruginosa Bacteriophage from Agriculture Drain Water

Amina A. II. and M.K. Amin

...xt-align: justify;">Five bacteriophages infecting Pseudomonas aeruginosa strain were isolated from agricultural drain water of Zagazig city. One of them designated as PAI has been characterized. The ability of phage PAI to form plaques with strain PAOI was examined. Two kinds of plaques (turbid and clear) have been observed. The average number of turbid plaques was 2.52 x 109 pfu/ml whereas of clear plaques was 9.96 x 107 pfu/n) l. The phage was able to lysis ...
Isolation and Characterization of a Pseudomonas Putida Bacteriophages

Mayada A. Abd Elgalell; B.A Othman2; Th. Radwan, 1 and Amal S.M. Abo-Sinna3

Evolution of super-drug resistant microbial strains: mechanisms and strategies for containment

SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3

...as, probiotics, CRISPRs, bacteriophages, nanotechnology and phytochemicals.

...
Using Bacteriophage in Poultry Diet as Therapeutics Against Escherichia coli in Broiler Chickens

Dheyaa Ali Aljuhaishi*, Firas Hussain Kadim Albawi 

...ml and were treated with bacteriophage (3.2×108PFU/g) through feeding. In the G2 group, chicken were orally inoculated with E. coli at a dose of 0.5 ml containing 7.5x106 CFU/ml and were treated with enrofloxacin 100mg/kg and neomycin 200mg/kg orally through drinking water. In the group G3, chickens were orally inoculated with E. coli at a dose of 0.5 ml containing 7.5x106 CFU/ml and were treated with both combination bacteriopha...
Bacteriophage endolysins and their role in eradication of bacterial biofilms

Anugrah Masih; Aditi Singh*

...by combining
bacteriophage with antibiotics for a synergistic impact and employing the phage-lysin
efficiently. The aim of this study was to explore the potential efficacy of phages and lysins
alone and/ or in conjunction with antibiotics to combating biofilm conformation and
eradication. This review article is broadly divided into two parts; the first section focused on
molecular mechanism of biofilm...
Application of bacteriophages isolated from sewage to control urinary O157:H7 Escherichia coli and several bacterial uropathogens

Salwa M. Masoud1; Hend A. Refat2; Nada S. Sayed2; Mohamed K. Abd-El Aal2; Ahmed A. Dosocky2; Zeinab S. Mohammed3; Mohamed A. H. Abdel Wahab3; Dina M. Mekawy3; Ali I. Ali4; Basma A. Atya4; Katren T. Welliam4; Rehab M. Abd El-Baky1, 5; Zeinab S. Hashem1*

Novel Research in Microbiology Journal (2020), 4(5): 1005-1014
...he effect of waste water bacteriophages on the growth of some multi-drug resistance Escherichia coli and other bacterial uropathogens. Urine samples were collected from 30 UTI patients at Minia University Hospital, Minia, Egypt. Twenty Escherichia coli strains were isolated from UTI patients. The Kirby-Bauer disc diffusion method was used to determine the isolates antibiotic susceptibility. The isolates showed resistance to ciprofloxacin and levofloxacin by 70...
Bacteriophages as affordable solution for treatment of multidrug resistant bacteria, and their recent potential applications

Ghadah A. Alsubhi1,2*

Novel Research in Microbiology Journal (2021), 5(6): 1405-1414
...p;

The impact of bacteriophages as antibacterial agents was known after their first discovery in 1915, and they were then employed to treat the bacterial infections. However, the discovery of penicillin in 1928 quickly overshadowed bacteriophage therapy, and paved the way for its large-scale production in the 1940s to enter the era of antibiotics. In recent years, resistant bacteria have contributed to increasing the...

Exploiting biological tools for post-antibiotic era: novel sustainable strategies against antimicrobial resistance

Omnia Karem Riad*

Novel Research in Microbiology Journal (2024), 8(6): 2734-2749
... predatory bacteria, and bacteriophages, which are powerful tools valuable to fighting resistant microorganisms with fewer chances of resistance development. Naturally synthesized products such as antimicrobial peptides and bacteriocins revealed successful treatment options. Additionally, the Clustered regularly interspaced short palindromic repeats (CRISPR-Cas) system is a gene-editing tool that can re-sensitize the resistant bacteria. Vaccination prevents in...

Novel Research in Microbiology Journal

February

Vol. 9, Iss. 1

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