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Prevalence, Conventional and Molecular Characterization of Salmonella Isolated from Chicken Farms and Slaughterhouses

Prevalence, Conventional and Molecular Characterization of Salmonella Isolated from Chicken Farms and Slaughterhouses

Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1* 

1Abu Suwayr Veterinary Medical Unit, Directorate of Veterinary Medicine, Ismailia 41513, Egypt; 2Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Ain Shams University, Shubra Al Khaimah, Cairo 11241, Egypt; 3Department of Animal Hygiene, Zoonosis, and Animal Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.

*Correspondence | Essam S Soliman, Department of Animal Hygiene, Zoonosis, and Animal Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt; Email: [email protected] 

Figure 1

Biochemical positive tests for the isolated Salmonella. A. Triple sugar iron agar (N, negative tube; P, positive with the blackening, and P, positive with the fermentation action with gas production only). B, Lysine iron agar (N, negative tube; P, positive tube with the partial fermentation action, positive tube with the complete fermentation action, and P, positive tube with the blackening. C, Simmons citrate (N, negative tube and P, positive tube with the development of the blue color), D, Methyl red (N, negative tube and P, positive tube with the development of the red color). 

Figure 2

Gel electrophoresis UV images showing representative samples with positive bands (1 and 4: 10) and negative samples (2 and 3) compared to the ladder, positive, and negative controls. 

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Advances in Animal and Veterinary Sciences

November

Vol. 12, Iss. 11, pp. 2062-2300

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