Yashab Tur
Science, Religion and Culture, Vol. 2, Iss. 2
... about the emergence and propagation of life on earth which, to the lay believer, seemed outright un-Godly. Marwa Elshakry, now an Associate Professor of History at Columbia University, explores the encounter of the Arab world with these and many other Western technologies and ideas in her wide-ranging Reading Darwin in Arabic through a panoramic lens, which then zooms in to focus on specific individuals. Thus we meet Free Masons, missionaries, colonial agents...
John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure
...) habitats in Leptospira propagation. Influential habitats would serve as key targets for interventions. To achieve this aim, we have designed a pseudo-longitudinal survey to be undertaken for two years in Kibera slums in Nairobi city Kenya. Human and animal blood and urine samples will be collected at the household level whereas soil and water samples will be collected at the community level. Attempts will be made to isolate Leptospira bacteria from urine, so...
Riaz Alam* and Muhammad Sajid
...tivars regarding asexual propagation through air-layerage. The daughter saplings of cultivar Manzanilla took less number of days (47.94) to root appearance, produced more rooting percentage (38.89%), number of roots (4.31), root length (4.61 cm) and root weight (1.77g) with more number of re-sprouts (3.18) and shoot length (5.28 cm). The time intervals of air layering also significantly influenced the rooting and growth attributes of olive cultivars. Mid Augus...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5
...tro culture protocol for propagation of an endangered medicinal plant Neurada procumbens L. from Cholistan desert in Pakistan. Nodal segments from healthy grown plants were used as explants and cultured on standard Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root prol...
Muhammad Muddassir Ali1,2 and İbrahim Hakkı Ciğerci1,*
...eserved for their proper propagation. Thermposis turcica is endemic to Turkey. It’s general anti-oxidant and anti-cancerous activities are explored, but no study has been observed on liver cancerous cell line in term of its genotoxicty. So, genotoxic evaluation was carried out for the alcoholic and hexane extracts of T. turcica. Methanol extracts showed the highest DNA damage (20±1) at 200 µg/ml concentration and 11.67±2...
Mehboob Arslan* and Xin Yang
...ival, proliferation, and propagation inside the host cell, viruses modulate certain metabolic pathways to destabilize the host metabolism. These alterations in host metabolic signaling as a result of virus-host interaction generate a favorable environment for the viral pathogens. This phenomenon is a hotspot for investigations in order to improve the diagnosis and drug development of future antiviral therapeutics. In fact, metabolism is considered as a crucial...
Qaisar Jamal*, Akram Shah, Syed Basit Rasheed and Muhammad Adnan
...e noted. Acquisition and propagation of axenic amastigotes was assessed. During promastigote culture, log, mid-log, and late-log phases were observed respectively on day 4, 5 and 6. The stationary phase was observed on day 7. The day following inoculation, most of the promastigotes were nectomonads having long and slender bodies with roughly uniform width from anterior to near posterior end, having as long flagella as one and a half to twice the body. The nect...
Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui2
...xt-align: justify;">Micropropagation is a phenomenal technique to serve rapid multiplication of vegetatively propagated plants through in vitro culture. This study was conducted to establish the optimal concentrations of plant growth regulators and sucrose for micropropagation of sugarcane. Sugarcane is cultivated through cane sets and therefore, multiplication of any new genotype through traditional practices requires ...
Mohammad Enamul Hoque Kayesh1,2*, Mohammad Tufazzal Hussan3, Md. Abul Hashem2,4, Mohammad Eliyas5 and A.K.M. Mostafa Anower1
Lumpy Skin Disease Virus Infection: An Emerging Threat to Cattle Health in Bangladesh
...sh are favorable for the propagation of vectors, which should influence the rapid transmission of LSD in the cattle population of Bangladesh. Therefore, LSD may appear as a big challenge to cattle health in Bangladesh in the near future. Consequently, it is very important to take strategic plans and immediate control measures for LSD outbreak control and prevention in Bangladesh. In this review article, we will focus on epidemiology and modes of LSDV transmiss...
Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5
Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...me the hindrances in the propagation of Stevia plants using in vivo methods, an alternative technique of propagation using in vitro methods is needed. The micropropagation method with the RITA® (Recipient for Automated Temporary Immersion System) is used in the production of a large amount of stevia biomass in approximately a short period. The forming of flowers is one of the limiting ...
Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo
The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...are capable of continued propagation. Azotobacter spp. with nematicidal activity could control root-knot nematode, Meloidogyne incognita. The development of such strains by genetic manipulation by transferring gene (s) required for saponins production is effective in increasing crop yield together with fixing nitrogen. Escherchia coli is still used as a host for the recombinant DNA technology – based industrial production of proteins and peptides to mana...
Inam Ul Haq1*, Humara Umar2, Naeem Akhtar3, Muhammad Azhar Iqbal2 and Muhammad Ijaz1
Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review
...xt-align: justify;">Micropropagation is one of the most significant applications of biotechnology in the field of horticulture. This technique is used for proliferation of olive (Olea europaea L.) tree which is globally cultivated for centuries and its value added products are consumed worldwide. The traditional propagation techniques of olive are uneconomical with wastage of time while micropropagat...
Muhammad Ihtisham1, Noor Amjad2, Muhammad Nauman3, Asghar Ali3, Khawar Riaz3, Muhammad Sajid3, Muhammad Owais Shahid*3
...mmon and cheap source of propagation. However, several plant species have water-impermeable hard seeds which take a long time to germinate. The hard seed coat can be scarified successfully through acid treatment. Sophora is a beautiful ornamental plant and its seeds have the same issue. In this experiment, seeds of Sophora secondiflora were treated with H2SO4 and then sown at different dates. Both H2SO4 scarification and sowing time had a significant effect on...
Anwar Ali1,5*, Horia Shaukat2, Munir Ahmed3,5, Ahmed Bostani4 and Syed Ashiq Hussain3
... stimulation fastens the propagation and termination of rigor mortis hence decreasing the processing time and fatigue. After electrical stimulation, the muscles become paralyzed and the rigor mortis is dispatched to reduce the time spent in the muscle and his fatigue. It is also found to enhance meat tenderness and to boost meat sensory attributes. But it may also have adverse effects on some meat quality characteristics like holding capacity of water and othe...
Salma Javed and Samreen Khan*
...only. On the first week, propagation rate was noted approximately 2.5x10³ followed by 3.9x10³ on the second week and finely after three weeks incubation period the greatest multiplication rate estimated up to 8.9x10³; however, the same did not appeared on Aspergillus niger and result was found negative. The main purpose of captioned study was to report mass culturing of parasitic Aphelenchus avenae and their relationship and further explore thes...
Tahseen Ullah* and Noorul Amin
...m is recommended for the propagation of Berberis lycium from cutting....
Asmaa A.M. Abd El-Ghani *; Ahmed B.M.; Abdel-Raouf A. ; Nagwa S. Ata* and H.A. Hussein
...MEM) were used for virus propagation and titration. The
effects of two concentrations of Salvadora persica on viral growth in MDBK cells as
well as cytolytic activity of BHV-1 were evaluated at different times pretreatment
,simultaneouslly and after adsorption. The results demonstrated that the safest
concentration (non-cytotoxic) of the plant extract under study on MDBK cells was 0.1
mg/ml.The plan e...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
...intain the stability and propagation of the cloned gene then the cloned plasmid isolated to be transformed again in Ecoli BL21 cell which include the T7 promotor for expressing the glycoprotein D, western blotting carried out on the induced culture at different expression times using the alkaline phosphatase labeled N-terminal anti histidine monoclonal antibodies revealed single bands at the expected size, the same samples have been reacted with serum collecte...
Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1
... of
pathogen propagation and disease development.
...
Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2
...susceptible cell for the propagation of LSDV with best harvesting time
72hr P.I to obtain a maximum titer for subsequent vaccine production.
...
Muhammad Rafique1, Muhammad Azhar Iqbal2, Inam Ul Haq1*, Muhammad Ramzan Anser2, Humara Umar2 and Muhammad Ashraf Sumrah2
...rried out to standardize propagation technology concerning optimization of light intensity under greenhouse conditions. The current study was conducted at Izhar farms (Pvt.) Ltd. under the collaboration with Barani Agricultural Research Institute (BARI) Chakwal to find out the appropriate light intensity for successful olive propagation under greenhouse conditions. The data regarding days to root initiation, number of roots,...
Mazhar Ullah and Mohammad Sayyar Khan*
... for the effective micro-propagation of sugarcane. Various factors affecting tissue culture must be evaluated and optimized to produce maximum callus and regenerated plantlets. In this research, different auxins [Dichlorophenoxy Acetic acid (2,4-D), Nephthalene Acetic Acid (NAA)], cytokinin [Benzyl Amino Purine (BAP)] either alone or in combination with each other and carbon sources [sucrose, glucose and fructose] with varying concentrations (2, 4 and 6%) were...
Muhammad Taqi1, Shazia Erum2, Shamaila Rasheed2, Sadar Uddin Siddiqui2 and Shakeel Ahmad Jatoi2*
...for large-scale and fast propagation of C. tuberculata through in-vitro organogenesis was carried out at in-vitro lab, Bio-resources Conservation Institute, NARC, Islamabad, Pakistan. Nodal-tips as explants were grown on MS salts enriched with diverse plant growth regulators including BAP, NAA, Kin and 2,4-D, alone as well as in combinations. The highest growth response for callusing 78.3% was observed in BAP (0.5mg/L) in combination with Kinetin (0.25mg/L) +N...
Syarif Husen1*, Devi Dwi Siskawardani2, Setiawan Deny Rexmardi1, Erny Ishartati1, Muhidin Muhidin1, Jumpen Onthong3 and Ivar Zekker4
...wingmediacompositiononthepropagationofstemcuttingpotato(G0)plantedinthe screenhouse.ConductedinSumberBrantasVillage(1700ma.s.l.),BumiajiRegency,City ofBatu,EastJava,Indonesia,thisresearchutilizedRandomizedCompleteBlockDesign withsevengrowingmediacompositionsandfourreplicatesinamicro-cuttingplanting.The growingmixmediacompositioncontainedhuskcharcoal+cocopeat+bokashi(goat manure) + bokashi(cowmanure), andhuskcharcoal+ cocopeat+bokashi(cow manure) + bokashi(poul...
Hanaa H.A. Gomaa1*, Dalia Y.Z. Amin1, Mona A. Ismail1 and Khalid A. El-Dougdoug2
...iana glutinosa for virus propagation. Fresh wood cuttings (4-6 nodes) were collected from naturally infected fig plants. Healthy fig plants were grafting-inoculated with chip budding of infected fig plants and kept in a greenhouse. Symptoms were confirmed by DAS-ELISA. Strips of infected and healthy N. glutinosa leaves were viewed under light microscope. Extracted infectious sap of N. glutinosa leaves was examined using Transmission Electron Microscope. Total ...
Imran Muhammad Siddique1, Naseem Sharif1*, Abdul Ghaffar2, Mudassar Naseer1, Ammara Noreen3, Naseem Akhtar4 and Safeer ud Din5
...ugh vegetative method of propagation thereby avoiding inevitable heterozygosity and polyembyonic nature of citrus fruits....
A.S. Gamal El din1; Sohair I. El-Afifi2; A. S. Sadik 2; Nashwa M. A. Abd El-Mohsen1; and H. M. Abdelmaksoud1
...l Vector by ligation and propagation after transformation process in E. coli. (2) Isolation of plasmid DNA. then used restriction enzymes Bam HI and Bgl II to identify clones containing inserts. To confirm the fragment inserted of CP gene sequence. PCR was performed using specific primers for PVYN CP gene. (3) The gene expression was performed using 100 µM IPTG & 2 µM biotin and incubation for 4h / 37 oC to produce biotinylated protein for E. c...
S.A. El-Kewey1; R.A. Omar1; S.A. Sidaros2 and Samaa Abd El-Khalik3
...ssion through vegetative propagation reached 100% in both cultivars (Chinese and Balady) with different degrees of symptoms severity. Plant height, leaf area. fresh and dry weight of bulb decreased significantly with the increasing of severity symptoms on garlic plants of both cultivars. The electron microscope preparations of crude sap extracted from naturally infected garlic plants and negatively stained with 2% PTA showed flexuous rod-shaped particles 657-7...
Asim Faraz1, Amal AlKharusi2, Nasir Ali Tauqir3, Muhammad Shahid Nabeel4, Syeda Maryam Hussain5, Abdul Waheed1*, Muhammad Arslan Akbar6
...kh Mahni for rearing and propagation during period 2014-2016. The farm management worked to improve and propagate the breed of Thalli Sheep. Different management practices like identification, weighing, vaccination, deworming, dipping, spraying, hoof trimming, shearing, breeding, lab screening and weather management were practiced to increase the fertility and decrease the mortality percentage. Average birth weight was improved from 3.5 to 3.6, 4, 4.4 and 4.5 ...
Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5
...hnique to develop a mass propagation system for the effective multiplication and conservation of P. minima. Juvenile leaves were cultured on MS basal medium supplemented with 0.1, 0.5, 1.0, 2.0, and 5.0 µM thidiazuron (TDZ). As a result, 100% callus induction was achieved on the medium containing 1.0 µM TDZ. Notably, calli grown on 1.0 µM TDZ exhibited greater potential for shoot regeneration and proliferation in the growth regulator-free MS ...
Yanping Li, Fei Xu, Yongming Wang*, Yunyun Lv, Jinrong Shi, Biwen Xie, Wenyao Cai and Dian Liu
...eed to implement captive propagation programs to protect their populations. Here, we firstly carried out artificial breeding of S. superciliaris and S. reevesae, and hybrid combinations were designed as S. superciliaris ♀ × S. reevesae ♂, and S. superciliaris ♂ × S. reevesae ♀. Secondly, 19 microsatellite loci were used to evaluate genetic diversity between hybrid offspring and their parents. The mean polymorphic information content (PIC) a...
Nasratullah Baloch1, Naeem Tariq Narejo2, Hamida Narejo3, Muhammad Farooq Hassan4, Ghulam Abbas5, Shahnaz Rashid5, Muhammad Hanif Chandio6, Faheem Saddar7 and Majida Parveen Narejo8
...sh diversity, growth and propagation. Length-weight relationship of four dominant species was calculated as follows. Liza subviridis: Log a -0.23+2.20, Sardinella longicep: Log a -0.78+2.92. Silago sihama: Log a -0.70+2.20, Acanthopagrus berda: Log a -0.5+2.12. It was noted that from the equation above that Sardinella longicep were found in ideal condition in comparison to the other three dominant species, Liza subviridis, Silago sihama, Acanthopagrus berda. S...
Abdul Latif Rind1*, Shahid Hussain Abro1, Rameez Raja Kaleri2,3, Ghulam Mustafa Solangi4, Raza Ali Mangi5, Muhammad Anees Memon6, Depeesh Kumar Bhuptani7, Sana Noor8, Zainab Lanjar9, Zahid Ali Mangrio10 and Abdul Wahid Solangi1
...rol measures to curb its propagation....
Ahmed*, Amal A.; Khatab*, Eman A.H. ; Dawood*, Rehab A. and Ismeil*, Amira .M.
...r proliferation and micropropagation of the infected shoot clumps respectively. The plantlets were rooted in MS medium supplemented with (1.5 mg/l NAA ) . Plantlets regenerated from meristem measuring 0.1 mm and 0.2 mm yielded (90 and 80 % ) for CLV and (92 and 80 ) for CarVMV virus-free tested plantlets respectively,while larger meristems (0.3 and 0.4 mm ) were not effective . As well as the plantlets survival was (20 , 35 , 65 and 80% ) for meristem measurin...
Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy
...) for BEFV isolation and propagation on Vero, BHK21 cell lines and baby mice. Identification was done by both Indirect Fluorescence Antibody Technique (IFAT) on tissue culture and inoculated mouse brain, while immunoperoxidase technique was done on bovine lung. Haematological examination revealed leucocytosis accompanied with neutrophilia (immature form) and lymphopenia. Histopathological examination clarified the most common lesion of field cases in lung in t...
H. A. Hussein1, N.A. Mohamed2, F.M. Mohamed2 and M.A. Shalaby1
M. Imran Kasana, Rashid Iqbal Khan*, Noor Ullah Khan, M. Noman, Shahid Ali, Saima Mumtaz, Shamaila Rasheed and M. Qamar-Uz-Zaman
...riod. Therefore, asexual propagation via grafting is the most appropriate method for producing high-quality, true to type plants. The current study was planned to evaluate four different grafting techniques (Cleft, Tongue, Patch and T-budding) in ten different cultivars of avocado. The outcomes suggested that Cleft grafting was most suitable one among all other evaluated techniques. The highest survival rate (27.40%) was recorded in cleft grafting. Moreover, v...
