Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
...rotein in the nucleus of oocytes which can promote in vitro nucleosome formation. In this study, to improve the efficiency of SCNT in Banna Mini-pig Inbred Line (BMI), we obtained the recombinant nucleoplasmin (Npm) by using a prokaryotic expression system and compared the difference on the developmental effects between exogenous Npm and its structural analog, polyglutamic acid (PGA). We chose the pMD18-T vector for Npm expression and transformed E. coli BL21 ...
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
...the embryos derived from oocytes matured in 20% and 40% SFF supplemented media. HSPB1 expression was significantly increased in group matured in 20% SFF. The relative expression of IL-6, LAMA1 and FGF4 genes did not show any difference between groups. In conclusion, IVM medium supplemented with 10% SFF showed the best rate of blastocyst development.
...
Şehriban Çek Yalnız 1,* and Erdal Yilmaz2
... theca of atretic mature oocytes was hypertrophied and persisted to form the interstitial cells, whereas the granulosa cells were regressed and disappeared. Erythrocytes and leukocytes were also detected at the advance stage of atresia. Apoptosis in the granulosa cells were clearly detected. The chromatin condensation against the nuclear envelope, cell shrinkage, surface blebbing and generation of apoptotic bodies by cell fragmentation were evidence of apoptos...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
... reproduction (IVF). The oocytes are inevitably ruptured by the microinjector in the process of microoperatin. To study the effects of mechanical damage from microopetations on embryo development, we analyzed 60 the spent culture media of porcine parthenogenetic embryos experienced different types of microopetation using Raman spectroscopy. The results showed that Group II with the puncture of egg zona and plasma membrane had the highest cleavage rate and blas...
Asima Azam1, Asma-Ul-Husna2, Saima Qadeer3, Qaisar Shahzad4, Rabea Ejaz1, Nemat Ullah5, Tasneem Akhtar4 and Shamim Akhter2,*
Abd El-Nasser Ahmed Mohammed1,2,*
... control. The quality of oocytes and reproductive performances were improved due to N. sativa oil supplementation. N. sativa oil rescued the depression in oocytes number and quality after ovarian transplantation. N. sativa oil could not improve hypothermia and hyperglycemia due to general anesthesia. In conclusion, supplementation of N. sativa oil could benefit animals’ health and reproducti...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...d breaks (DSBs) in mouse oocytes and resultant early embryos. Germinal vesicle (GV) oocytes were first cultured to monitor the progression of germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) during in vitro maturation (IVM), then the harvested metaphase II (MII) oocytes were parthenogenetically activated to evaluate pronuclear (PN) formation of parthenogenetic embryo...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...esis, follicular growth, oocytes maturation, ovulation and corpus luteum formation. The aim of this study was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detected in the Pasundan bLHR/Hha...
Arslan Mahmood Ahmad1, Muhammad Ameen Jamal1, Abdul Sattar1, Aamir Ghafoor2, Aqeel Javeed3 and Amjad Riaz1*
...ificial activation mouse oocytes; however, limited reports are available to evaluate its negative impact. The objective of this study was to optimize the concentration of SrCl2 for artificial activation of mouse oocytes and to evaluate its toxic effect on early embryonic development.Our results indicated that higher percentage of activation of mouse oocyte was achieved with 10mM and 15mM for period of 3 h and 6 h ...
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...as6 was expressed in the oocytes, especially the nucleus, from the primary to preovulatory stages. The further immunofluorescence results showed that Gas6 was expressed thoughout the in vitro matured porcine oocytes, and the staining signal decreased in the first 12 h but then increased during the process of oocyte maturation. QRT-PCR analysis revealed that Gas6 mRNA was continuously expressed during the...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3*, Abd El-Nasser Ahmed Mohammed3
...n. Molecules transfer to oocytes through gap junctions of cumulus cells. Therefore, cumulus cells and their secretion, follicular fluid, effect on oocyte maturation and developmental potential of the resulting embryos in vivo and in vitro. Because of the importance of cumulus cells on follicular development, this review was made to collect and consolidate the current knowledge of cumulus cells effects on maturation of intact and reconstructed
Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1
...gative cows. The intact oocytes were clearly pronounced only in the negative animals. The fallopian tubes are free from any pathological lesions in the negative animals. Immunohistochemical, BVDV antigen was detected in ear notch, ovaries, fallopian tubes and uterus of one cow (1/30) (PI animal). Whereas, BVDV antigen was detected only in the genital organs of acutely infected animals (2/30) but ear notch was negative. The positive BVDV antigen was clearly ob...
Rashid Al Zeidi1, Haitham Al Masruri2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Al-Hassan Mohammed4*
...x process. Maturation of oocytes include several changes at the cytoplasmic and nuclear levels that makes the oocytes able to undergo fertilization, pre-implantation and post-implantation embryo development. Oocyte meiotic maturation in vivo starts after LH hormone surge whereas it starts in vitro upon release of oocyte from the ovarian follicle. Rodent oocyte maturation lasts 15-17hr. whereas that of ruminant oocyte lasts 2...
Abd El-Nasser Ahmed Mohammed*, Aiman Al Mufarji and Sultan Alawaid
...ial follicles consist of oocytes surrounded by a single layer of pregranulosa follicular cells and they remain dormant in the meiotic prophase I stage. Primordial pregranulosa follicular cells initiate activation of primordial follicle and govern the development of dormant oocytes. The primordial follicles take about 6 months to grow and develop to ovulatory graafian follicles in cattle and humans. Growth of preantral follic...
S. Riffat†, S. Kumar and A. Soomro
...gnificant suppression of oocytes and testis development in infected individuals....
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
... to fertilize the mature oocytes which subsequently underwent culturing in a simple culture medium (KSOM). The results showed that percentage of matured oocytes, fertilized oocytes, 8-16 cells developments, blastocyst in 100 µM cysteine group (73.00%, 54.79%, 30.14%, and 17.81%, respectively) were higher than the control group (48.00%, 37.50%, 18.75%, and 10.42%, respectively) and 20...
M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1
...s produced in vitro from oocytes matured in stressful conditions of elevated concentrations of non-esterified fatty acids (NEFAs). The maturation conditions in the current study were resemble the situation in the oocyte microenvironment of metabolically stressed lactating dairy cows during the post-partum period where the negative energy balance occurred. Oocytes were in vitro matured under elevated levels of NEFAs for 24 h ...
Beenish Shahid1* and Muhammad Ijaz Khan2
...er recovery of competent oocytes in buffalo species limits the commercialization of in vitro embryo production technology in field conditions. The present study aimed to improve the cytoplasmic and nuclear maturation of in vitro matured oocytes of Nili Ravi buffalo in the presence of hormones. The denuded oocytes (DOs) obtained by repeated pipetting were collected from 2-8mm follicles. A s...
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
... slaughterhouse and were oocytes mature before fertilization using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and embryo culture media were collected using a disposable syringe and centrifuged, then supernatant in the collection, stored and frozen ...
Sri Rahayu*, Muhaimin Rifa’i and Widodo
...9), which is secreted by oocytes, is a paracrine factor that plays a vital role in follicular development. We performed a computational biology analysis to determine the role of GFD9 in mammalian ovarian function. We constructed a three-dimensional model of GDF9 using I-TASSER and human GDF9 protein sequences obtained from UniProt. We also analyzed GDF9 gene expression levels using GENEVESTIGATOR. Furthermore, we identified the proteins that interact with GDF9...
Erni Damayanti1, Herry Sonjaya2*, Sudirman Baco2 and Hasbi Hasbi2
...s produced in vitro. 542 oocytes were matured in TCM 199 medium, were fertilized and were cultured using CR1aa medium for 48 h. After 48 h of culture, the embryo developed into 2, 4, 8 and 16 cells. Furthermore, it was grouped based on the stage of development, each of which was re-cultured for 48 h. After that, its development was re-evaluated and H2O2 measurements were taken. The 2-cell embryo was not able to reach the morula, while stage 4-, 8- and 16-cell ...
Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*
...tion of Egyptian buffalo oocytes has not been determined yet. This study aims to estimate the effects of supplementing LC on the in-vitro fertilization (IVF) of Egyptian buffaloes. We evaluated blastocyst and apoptosis rates in order to substantiate the potential development of cumulus oocytes–complexes (COC) and determine the effect of LC in reducing the occurrence of apoptosis under in vitro maturation (IVM) conditio...
Ferry Lismanto Syaiful*, Jaswandi Jaswandi, Mangku Mundana, Zaituni Udin
...ration of Pesisir cattle oocytes indicate the development of GV (Germinal Vesicle), GVBD (Germinal Vesicle Breakdown), M-I (Maturation-I) and M-II (Maturation-II) cells in different culture media as follows: B-O medium (14.29%, 0%, 33.33%, 57.14%), mB-O (17.65%, 0%, 23.53%, 58.82%). The results of in vitro fertilization rates using different culture media at various incubation periods were as follows: B-O medium at 6, 12, and 18h yields 57.14%, 52.17%, and 48....
Muhammad Tahir1, Muhammad Saqib1, Shahbaz ul Haq2, Shahrood Ahmed Siddiqui3,4, Khurram Ashfaq1, Urfa Bin Tahir5, Mughees Aizaz Alvi1*, Shujaat Hussain6, Talha Javaid1, Raheela Taj7, Muneeb Islam8, Imad Khan9, Asad Ullah9* and Shakirullah Khan10
...nts through ingestion of oocytes-contaminated herbage. The purpose of the current study was to report prevalence and access the risk factors associated with toxoplasmosis. A total of 652 specimens of blood were taken from 9 regions situated within the jurisdiction of Punjab province, Pakistan. The collected samples were labeled in order to have randomization and to pull 384 samples out of 652 harvested samples. Survey Tool Box was used to have representative s...
Sarhad Journal of Agriculture
September
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Cytochrome-P450 (CYP11B1 Gene) Polymorphism and its Role in Bovine Milk Producing Traits
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Investigations on the Feeding and Spawning in Bagarius bagarius from Manchar Lake District Jamshoro, Pakistan
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Milk Quality Improvement Program for Small-Scale Dairy Farmers in Angochagua, Ecuador
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