Ahmed Zein Mahmoud, Muaz Abdellatif and Ahmed Abdalla
...ts and camels. Sera from non-vaccinated sheep (n=683), goats (n=624) and camels (n=155) of all ages and sexes were collected in a cross-sectional study in Hail, Bagaa, Shenan and Ghazalah. Saudi Arabia. The seroprevalece was determined by NP-epitopes based competitive ELISA. The overall prevalence was 59.9%, goats had a significantly higher sero-prevalence of 75.3% compared to 59.4% obtained from sheep, whereas camels were seronegative. The prevalence of PPR w...
Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2
...ccinated and challenged, non-vaccinated and non-challenged and non-vaccinated and challenged indicated that MDA for H5N1 gradually decreased from 5.1 to 0.4 log2 and disappeared with in a month. While MDA for NDV remained 2.1 log2 until 28th day of age, the Geometric mean titers (GMT) for H5 were increased from the 7th day of age using both antigens (RE-5 antigen) and (S75/Egy/2015 antigen). Generally, GMT post-challenge was...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
...9, AIV H7 and AIV H5) in non-vaccinated wild and domesticated bird species across Pakistan. During January-December 2013, in total 700 samples were collected from different species of non-vaccinated birds inhabiting diverse ecological zones of Pakistan. Altogether, 507 tissue and swab samples were screened for the presence of viral RNA by reverse transcriptase polymerase chain reaction (RT-PCR). Same samples were further pro...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2
.../i> 4.39% as compared to non-vaccinated animals having 66.53%. Another risk factor age suggested that younger animals were more affected (60.17%) as compared to older ones (29.41%). It was also observed that animals in closed housing system were more affected as compared to open housed animals. When comparative efficacy of diagnostic tests was done then the results of RT-PCR was found significant (P>5), than complement fixation test.
...
Omer Baris Ince*
... that were classified as non-vaccinated, single-vaccinated, multiple vaccinated, aged 0-1 and 1-3 and male and female starting from the third month following the vaccination. In the study aimed to show the disease-related status of the selected region, to investigate the active virus circulation and determine the antibodies formed against the nonstructural proteins (NSP) of Foot and Mouth Disease and evaluation the carrier rate in the region and the risk of in...
Biomarkers for Pathogenic Clostridium perfringens in Small Ruminants of Khyber Pakhtunkhwa, Pakistan
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Naimat Ullah Khan3, Muhammad Asfandyar Khan2, Kashif Prince1,*, Mahboob Ali1, Ghazunfar Rashid1 and Azmat Ulah Khan2
...and kids population from non-vaccinated dams of selected districts of Khyber Pakhtunkhwa province, Pakistan. Samples were obtained from non-vaccinated lambs and kids with the signs of enterotoxaemia. The isolates were initially identified by colony characteristics, Gram staining, biochemical tests and CFU/g. Clostridium perfringens was isolated from 43.45% of lambs and 50.59% of the kids. Obtained isolates were furthe...
Naglaa F.S. Awad1, Gamelat K.F. Kotb2
...ample was collected from non-vaccinated young rabbit (45-day old) and designed here as SHAH
2015, whereas the second sample was collected from non-vaccinated adult dam (nine-month old) and
designed as SHMK2016. Hemagglutination analysis using human red blood cells type O revealed
that both isolates were non-hemagglutinating. Sequence analysis revealed that both isolates shared
...
Abdou Nagy1, Fatma Abdallah1, Kareem Sweed2, Ahmed Salama3, Mohamed Omar4
...roprevalence of aMPV for non-vaccinated chicken and duck
flocks from six provinces of Egypt (Ash Sharqiyah, Ismailia, Qalyubia, Menufia, Sohaq and North-
Sinai) and determine its economic effect.
Methods: Serum samples were collected from non-aMPV vaccinated apparently healthy 40 chicken
(23 broilers and 17 layers) and 8 duck (6 Pekin and 2 Muscovy) flocks and then, tested with
commercial indirect aMP...
Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2
...ccinated
and non-vaccinated animals needs to be addressed.
...
Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2
...es and group-4 served as non-vaccinated control. The humoral immune response evaluated
serologically by using hemagglutination inhibition test (HI), serum neutralization test (SNT), and
indirect ELISA.
Results: There was detectable TRT antibody titers by SNT in the first week post vaccination as 4 log2
in group-1 and 2 log2 in group-3. Both groups showed peak SNT titer (256 log2) of TRT virus by the
t...
Sonali Menamvar1,2,3, Kirubakaran Vinod Kumar1, Veeregowda Muniveerappa Belamaranahally2, Yella Narasimha Reddy3, Rathnamma Doddamane2, Shrikrishna Isloor2, Ramesh Poojari Thimmaiah2, Gurrappanaidu Govindaraj1, Bibek Ranjan Shome1, Vinayagamurthy Balamurugan1*
...serum samples from eight non-vaccinated dairy herds. The collected serum samples (n=56) were examined for detection of leptospiral antibodies using the Microscopic Agglutination Test (MAT), the gold standard serological test. The Chi-square and odds ratio analyses were employed to identify the important risk factors for leptospirosis in dairy farms. The study revealed that the seroprevalence of bovine leptospirosis at an individual animal and farm level was 39...
Arsalan Maqbool1,2, Faez Firdaus Abdullah Jesse1,3*, Eric Lim Teik Chung3,5, Abd Wahid Haron3, Mohd Azmi Mohd Lila5, Bura Thlama Paul3,4, Khaliq ur Rehman Bhutto3,6
...3 groups (n=4), control, non-vaccinated and vaccinated in each season. After acclimatization and synchronization procedure, the vaccinated group was administered with 2 mL of alum-precipitated pasteurellosis vaccine while non-vaccinated and control groups were administered with 2 mL of phosphate-buffered saline (PBS) via the intramuscular route. At week 2, both non-vaccinated and the vacci...
Effects of Antibiotics on Growth Performance, Immune Response, and Intestinal Microflora of Broilers
Shahan Azeem1*, Muhammad Akram Muneer2, Liaquat Ahmad3, Sameera Akhtar1 and Talat Naseer Pasha4,5
...ciency of non-medicated, non-vaccinated chickens was equal to the flavomycin-medicated chickens but better than zinc bacitracin and lincomycin-medicated chickens. Additionally, the non-medicated, non-vaccinated chickens were the most economical to raise. Overall growth-promoting antibiotics did not interfere with the broiler’s immunity, altered total intestinal microflora counts, or improved feed efficiency. ...
Muhammad Mohsin Abbas1*, Sajjad Ur Rahman1, Muhammad Abubakar2, Muhammad Imran Arshad1 and Khurram Ashfaq3
...ine group as compared to non-vaccinated bovine with (12.93%), (9.45%) and (5.22%) respectively across the district. More specifically the cow population was carrying maximum seroprevalence of type O with (60.55%) compared to buffalo with (36.58%). It was concluded that serotype O was more prevalent in district Bahawalpur followed by serotype Asia 1 and serotype A and cow population was found more susceptible than buffalo....
Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2
...as positive and negative non-vaccinated controls. At 30 days of age groups A, B and C were challenged with VNDV genotype VII1.1 isolate, where the clinical manifestations, gross post-mortem lesions, Humoral immune response and also quantification of virus shed post-challenge (PC) via real-time QRT-PCR were all screened and precisely recorded. The results revealed that, group A chickens developed the highest humoral antibody titers throughout the vaccination sc...
Madboulyl , H. M.; Tamami , S.M.; Abd Elmoneml , A.S.; Husseinl , A.S. and Arafa2, A.M.
...k samples collected from non-vaccinated flocks (608 serum samples were obtained from broiler chickens from El-Fayoum, Beni suef and Giza, 40 turkey poults serum samples from Al Behira and El Monofia, 278 chicken egg yolk samples from El-Monofia, Dommiat, Alexandria and Al Behira and 675 turkey egg yolk samples from El-Fayoum and Beni suef Governorates) were tested by using ELISA. The percentages of positive reactors of chicken sera and egg yolk samples were 57...
Hala M. El-Makaky*•, Sherif, N.A.;Fekria El-Bordiny* and Taha, M.M.*
... third group was kept as non-vaccinated control. Evaluation of the prepared vaccine was done by measuring both cell-mediated and humoral immunity. The results of both T-lymphocyte transformation and ELISA tests revealed that the prepared vaccine induced good protection and has good potentiality for use in chicken in the Egyptian poultry industry....
Laila,A. Sedeek; Fatma,S. Mohamed and Manal Abo El-Yazyed
... separate group was left non-vaccinated and served as control. All animals remained clinically normal throughout the whole experiment period. A satisfactory humoral immune response to both virus vaccines in vaccinated animals was identified by SNT and ELISA. It was found that there is no difference in the immune response among different vaccinated groups and there is no drawbacks noticed from combination of the two vaccines in the field....
Wahid Hussein El-Dabae*, Eman Shafeek Ibrahim, Eslam Gaber Sadek, Mai Mohamed Kandil
...demics in vaccinated and non-vaccinated flocks are still recorded, indicating an urgent need to generate a new vaccine candidate. In this study, the live Orthoavulavirus javaense (AOAV-1) genotype VII.1.1 strain (CH-EGY-GIZA-VVTNRC-2021) (Genbank accession number MW603772) was attenuated by combining passaging with limiting 10-fold dilutions in the allantoic cavity of embryonated hens eggs for 20 passages. The attenuated virus was used to vaccinate 20 SPF chic...
Md Imran Hossen1, Shakhe Reju Ana Boishakhe1 and Mohammad Enamul Hoque Kayesh2*
...t frequently recorded in non-vaccinated young local male domestic cats. Anorectic condition, frequent vomiting, diarrhea, and different level of dehydration were recorded as the prominent clinical signs in FPV infection. Fluid therapy (5% dextrose saline and 0.9% saline), antiemetic (ondansetron), proton pump inhibitor (pantoprazole/esomeprazole), antibiotics (ceftriaxone or metronidazole and ceftriaxone) and multivitamins were suggested as the supportive trea...
Qurat-ul-Ain1*, A. Ahmad2, Farhat Nazir Awan3, M. Rabbani1 and M. Hassan Mushtaq4
...hile group C was kept as non-vaccinated control group. Blood samples were taken from each group after every 2 months up to one year post vaccination and tested through Ab capture ELISA. Overall, 23.7% sero-positivity of BVDV was observed in 5 agro-climatic zones with highest in the low-intensity zone (34%) followed by rice wheat (27%), cotton-wheat (21%), mixed-crop (19%) and lowest in the rain-fed zone (17.5%). Quarantine practices, housing management, aborti...
