Aneela Qureshi1, Ammara Ainee1*, Muhammad Nadeem1, Masooma Munir1,2*, Tahir Mahmood Qureshi1, Saqib Jabbar2
..., sensory attributes and microbiological properties of fruit cakes were examined during 30 days of storage. Results showed that with varying fiber content water activity, texture, color, moisture, fat, protein, ash, NFE and fiber content changed significantly (p<0.05). Gross energy level in cake which is desirable by consumers was reduced but high fiber cake was dark colored, low in volume and with increased hardness. Treatment (T3) containing 5....
Alim-un-Nisa1*, Sajila Hina1, Sania Mazhar1, Imran Kalim1, Ijaz Ahmad1, Naseem Zahra1, Shahid Masood1, Muhammad Khalid Saeed1, Qurat-ul-Ain Syed1 and Maida Asif2
...and 45oC. The microbiological study of the extracted color and candies prepared in laboratory and dyed with T. erecta extract color showed that the color extracted from T.erecta itself do not promote microbial growth rather it has antibacterial activity. The lethal dose and toxicity determination of the color extracted from marigold showed that it is safe for consumption as no clinical symptoms were observed in rabbits after giving the...
Ambreen Akhtar Saddozai, Saeeda Raza* and Shahzad Arshad Saleem**
MICROBIAL COUNT AND SHELF LIFE OF STRAWBERRY JUICE
...stigate the influence of microbiological activity on different quality factors of strawberry juice during storage. Strawberry was purchased from local market and its juice was kept for two weeks at room temperature to check its shelf life. Total plate count (TPC), pH, flavor, color, yeast and moulds were checked weekly. Quality changes of strawberry juice during storage can be caused both by microbiological and physiological...
Ambreen Akhtar Saddozai*, Amer Mumtaz*, Nouman Rashid*, Shahzada Arshad Saleem**, Saeeda Raza* and Muhammad Naeem Safdar*
COMPARISON OF INDIGENOUSLY DEVELOPED TANGERINE-ORANGE MARMALADE WITH BRANDED MARMALADES AVAILABLE AT LOCAL MARKETS
...alades were analysed for microbiological (TPC, yeast and mould), physico-chemical characteristics (pH, Brix, vitamin C and sugars) and organoleptic attributes. TPC count in both samples were nil. Yeast and -1 mould count was nil in branded while it was 600 and 400 cfug in orange and tangerine marmalades, respectively. Sugar contents, pH and Brix ranged from 14.97% to 59.70%, 3.00 to 3.46, 15.0 B to 60.2 B in branded and non-branded marmalades, respectively. Or...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...hogen,using conventional microbiological and molecular methods. The agar disc diffusion method was used to evaluate the antibiotic susceptibility. Virulence-associated genes were detected using PCR while association analysis was used to test the likelihood of strains carrying combinations of genes involved in toxin production and/or host immune evasion. Highest resistance was observed against beta-lactam group followed by cephalosporin, lincosamide, tetracycli...
Ali Karabayir1,*, Nazan Arifoğlu2 and Mustafa Öğütcü3
...ant clearly improved the microbiological quality of chicken drinking water, by reducing the total bacteria, coliform and E.coli counts. On the other hand, disinfectant addition decreased feed and water intake of all chicken strains. Furthermore, the results revealed that feed and water intake was strain dependent. Lower feed conversion ratio was observed for chickens provided with disinfectant supplemented drinking water. When considering the live weigh...
Ashraf M. Mounir, A.N. El shahat* and A.M. Abdul Azeem
... to achieve satisfactory microbiological quality and public health safety. Hence, examining the effect of gamma-irradiation on the contents of Boswellia oleo-gum resin and also investigating the role of gamma-irradiate frankincense aqueous extract (GFAE) against ISO-induced MI in rats were the two aims of this study. The total phenolic content and total flavonoids of frankincense has been significantly increased under the effect of gamma-rays in this wo...
Hafiz Muhammad Umer Qaisar1, Tanveer Ahmad2, Muhammad Rizwan3*, Muhammad Saqib1
Antimicrobial efficacy of combination of lincomycin and spiramycin (Lispiracin™) as systemic dry cow therapy for controlling bovine mastitis
...tion and purification of microbiological
cultures. The mean score of surf field mastitis test of G1 group before treatment
and at day 14thpost calving was significantly different (p<0.05) as compared to G2
group Somatic Cell Count was significantly reduced from 8.0 at dry off to 3.50
(P<0.05) after day14th post calving in lispiracin™ group. Quarter wise prevalence
(%) of clinical mastitis in G1 group was lower tha...
cultures. The mean score of surf field mastitis test of G1 group before treatment
and at day 14thpost calving was significantly different (p<0.05) as compared to G2
group Somatic Cell Count was significantly reduced from 8.0 at dry off to 3.50
(P<0.05) after day14th post calving in lispiracin™ group. Quarter wise prevalence
(%) of clinical mastitis in G1 group was lower tha...
Fatih Korkmaz1, Veysel Parlak2, Özgür Kaynar3, Arzu Ucar2, Gonca Alak1,* and Muhammed Atamanalp2
...test parameters, such as microbiological, physicochemical as well as the lowest mean values were identified in the coatings of the fillets with turmeric and cardamom (p<0.05). Control and quinoa groups resulted in increased number of bacteria and chemical values compared to other treatment groups (p<0.05). The turmeric coating also results in increased protein concentration compared to other coatings and control group (p<0.05).
...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...ed according to standard microbiological methods. Isolated GNRs were subjected to susceptibility testing against various antibiotics as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Carbapenem-resistant isolates were subjected to detection of Metallo-beta-lactamase production by Double Disc Diffusion test (DDDT) and Modified Hodge’s test. In 152 specimens, different species identified in order of their frequency were; Klebsiella p...
Muhammad Yahya1, Muhammad Afzal1, Muhammad Zeeshan Majeed1*, Imtiaz Sarwar1, Khurram Shehzad2, Muhammad Luqman3 and
Sher Muhammad Shahzad2
...oil physico-chemical and microbiological properties were also determined in order to find out the main edaphic drivers which can explain the population dynamics of these microarthropods. Results revealed that all major factors i.e. land-use types and categories, collection seasons and soil properties had a significant impact on the population abundance of springtails and mites. Agricultural soils harbored maximum abundance of both microarthropods follow...
Rida Haroon Durrani1*, Fareeha Akhtar1, Ali Ahmad Sheikh1, Munazzah Maqbool1, Aleena Kokab1, Kiran Imtiaz2 and Muhammad Ilyas Riaz3
Iqra Muzammil1, Muhammad Ijaz Saleem1, Amjad Islam Aqib2,*, Ambreen Ashar3, Syed Ashar Mahfooz1, Sajjad ur Rahman4, Muhammad Shoaib4, Muhammad Aamir Naseer1, Imran Khan Sohrani1, Javeed Ahmad1, Razaullah Saqi1, Fizzah Laeeq Lodhi1 and
Qaisar Tanveer5
...S. aureus through microbiological examination in the laboratory. Non-probability statistical tools conferred 42% (84/200, CI=35.37-48.93) prevalence of subclinical mastitis, 38.1% S. aureus (32/84, CI=28.45-48.79), 15.6% MRSA (5/32, CI=6.87-31.76), 46.9% haemolytic S. aureus (15/32, CI=30.87-63.56) and 34.4 % biofilm producing S. aureus (11/32, CI=20.41-51.69). Earthen floor type (OR=1.75, p=0.0996), poor drainage system (OR=...
Muhammad Suhail Ibrahim1*, Asif Ahmad1, Anwaar Ahmed1, Amer Mumtaz2, Muhammad Javaid Asad3, Saqib Jabbar2, Ahmad Mujtaba1 and Muhammad Nadeem4
... physical, chemical, and microbiological. Food safety ensures that what we eat, chew, or drink is safe for human health. The whole food chain should be monitored right from production to consumption. The public health burden of developing countries is increasing beyond their resources. In developing countries like Pakistan, food safety issues result in serious health issues. Diarrhea is perhaps the most important problem that is responsible for more than half ...
Alim-un-Nisa1*, Sajila Hina1, Imran Kalim1, Mohammad Khalid Saeed1, Ijaz Ahmad1, Naseem Zahra1, Sania Mazhar1, Shahid Masood1, Muhammad Ashraf1, Qurat-ul-Ain Syed1 and Rabia Shad2
Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski
...ed with the conventional microbiological method and with the use of multiplex PCR method. Both methods confirmed the overall prevalence of C. jejuni of 39.2%. In the second part of the study 108 confirmed isolates of C. jejuni were analyzed for the presence of resistance genes (CmeB, Blaoxa-61, tet(O), aph-3-1 and aadE). The analysis in the third part of the study was concentrated on the antimicrobial resistance of the C. jejuni isolates towa...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...as performed by standard microbiological techniques, and API 20E and for final confirmation multiplex PCR was performed using genus and species-specific primers. The susceptibility to various antimicrobial agents including the aminoglycosides was determined by the Kirby-Bauer method and minimum inhibitory concentration (MIC) to aminoglycosides was evaluated by the broth microdilution method. Moreover, the armA genes were studied by PCR followed by Sange...
Esraa Yosry Abdel Halim1, Hamdy El-Essawy1, Abeer Abdel Nasser Awad1, Mona S. El-Kutry2, Lamiaa Ibrahim Ahmed1*
...ted organoleptically and microbiologically. The sensory evaluation revealed that the majority of the examined labneh and cheddar cheese got excellent and good scores, while 90 % of milk powder samples got good scores, based on the overall acceptability scale. The mean titratable acidity percentages of milk powder, labneh and cheddar cheese samples were 0.169 ± 0.018, 0.935 ± 0.046 and 0.197 ± 0.013, respectively. M...
Muhammad Ammar Dilawar1,2, Hong Seok Mun1, Myeong Gil Jeong1, Eun Ju Yang3, Hyeoung Seog Park4 and Chul Ju Yang1,2,*
... lowest (P<0.05) meat microbiological count was seen in T4. These results indicate that certain ratios of plant extracts and liquid minerals can be used to enhance the performance and meat quality in poultry....
Weam Mohamed Baher*, Gamilat A. El said
...purpose of improving the microbiological quality of the final products.
Keywords | Chicken meat, Microbial load, Bacteriocin, Nisin, Gingerol
...
Ida Bagus Oka Winaya1*, Anak Agung Ayu Mirah Adi1, Putu Henrywaesa Sudipa2, I Gusti Ketut Suarjana2
...s in the leg joints, and microbiological tests suggest that post-weaning piglets suffer from streptococcosis.
Keywords | Histopathology, Meningoencephalitis, Post-weaning piglet, Streptococcosis, Streptococcus suis
...
Ochieng Onunga George*, Owuor Ndonga Millicent Florence, Mario Kollenberg
Fawziah M. Albarakaty*
...e most crucial phases in microbiological research because microbial resistance to chemical antimicrobial is the prevalent crisis in the therapeutic community. Objective: Therefore, this work aims to ascertain which phytochemical components can be recycled and added to the diet of animals while also examining the antibacterial, antifungal efficacy of plum (Prunus domestica L.) peel extracts on certain crucial аnimal microbes. Methodology: The antibacterial ac...
Jiahua Peng1,2, Shiyao Hua3* and Qian Wu4
...p between the intestinal microbiological axis of estrogen and metabolic defects in SD rat model of polycystic ovary syndrome (PCOS) induced by DHEA, and to explore the possible mechanism. Forty rats were randomly divided into four group, the model groups each of 10 the control group, the DHEA group (low dose, 15 μg/kg/d) and the DHEA group (high dose, 30 μg/kg/d). DHEA subcutaneous injection was used to construct the PCOS model of the polycystic ovary sy...
Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*
...an diet consequently the microbiological quality of milk is very important. Therefore, a study was carried out for the molecular characterization (identification) ETEC (Enterotoxigenic Escherichia coli) strains in both raw and pasteurized milk available in the market of District Kasur, Punjab (31.0896° N, 74.1240° E). A total of 65 samples of milk including 5 pasteurized milk samples from various sources were analyzed through scientific polymerase chai...
Rinat Islamov*, Dinara Turegeldieva, Altyn Rysbekova, Kuralay Sarmantayeva, Victor Semenuyk
...lts of the assessment of microbiological quality of laboratory animals from some universities and academic institutes in Almaty city, Kazakhstan. This is the first time that such work, involving several institutes, has been carried out. To do this, several pathogenic bacteria and viruses from the Federation of European Laboratory Animal Science Associations (FELASA) were selected. The main methods of testing biological samples from laboratory mice and rats wer...
SAEED AHMED*1, ZIA-UR-RAHMAN1, SALMA KHALID2, TARIQ KHAN1, ARSHAD IQBAL3
& ZAMARUD SHAH4
...justify;">To analyze the microbiological quality of drinking water from different sources (tap water, open wells, stream water, spring water and water supply networks such as municipal main storage tank) in rural communities of the Oghi; a total of 175 water samples were collected from the study area with 35 samples from each source. All the samples were analyzed for microbial contamination by pour plate method, membrane filtration technique and MacConkey agar...
Ahmad Jupri1*, Yuliana Vofi2, Faturrahman2, Immy Suci Rohyani1, Ernawati1, Bulkaini3, Djoko Kisworo3, Wardatul Jannah4
... analyze the quality and microbiological feasibility of drinking water sourced from Joben Springs, Montong Gading, East Lombok. The research was carried out in May-June 2021. The research method used was purposive sampling with sampling carried out at three points: the spring, the main reservoir, and the residents’ reservoir. The Coliform test was carried out using the Most Probable Number (MPN) method and Escherichia coli (E. coli) identification using ...
Balquees Kanwal1*, Syeda Saba Shah1, Syed Waqas Hassan2 and Farzana Shaheen3
...or standardization based microbiological techniques, while chain reaction technique (PCR) was used for detection and characterization of Stx-1 and Stx-2 genes. In total, 75 (46.8%) isolates were detected as E. coli. Among them, 14 (8.75%) isolates were tested positive for Stx genes (Stx-1 and Stx-2). The detection of pathotype of shiga toxin producing E. coli O157:H7 in chicken meat is a significant finding because this pathogen has been related to food borne ...
Natalia Shchur1,2*, Olha Chechet1, Tetiana Mazur2, Oleksandr Martyniuk2, Olga Gorbatiuk1, Halyna Buchkovska1, Iryna Musiets1, Diana Ordynska1, Olena Finkova3, Larisa Moskalenko3, Tetiana Ponomaryova-Gerasimyuk3, Maksym Lusta3, Vitalii Nedosekov2
...mples were analyzed by a microbiological method for the detection of Campylobacter according to the scheme of accumulation, isolation, isolation and identification according to DSTU ISO 10272-1:2007. As a result, 33 isolates of Campylobacter spp. were isolated, which is 1.6% of the total number of the researched samples. The most common phenotypes of antimicrobial resistance of the selected isolates were: Cip / Tet / Ery - 14 isolates from poultry, which ...
Suleman Irfan1*, Masood Rabbani1, Ali Ahmad Sheikh1, Sehrish Firyal2 and Arfat Yousaf Shaheen1
...he samples were assessed microbiologically for Total Viable Count, Total Coliform Count, and Salmonella detection. The mean log values of total viable counts of meat samples of traditional poultry shops, super markets and processed meat were 5.70, 4.65 and 3.60, respectively and significant (p < 0.05) results were obtained. The mean log values of total coliform counts in meat samples were 2.7, 2.31 and 2.11, respectively. E. coli was predominant 73% in coli...
Yaman Saad Fadhil
...4°C temperature. The microbiological properties (total plate count, Coliform count, Staphylococcus aureus), and the sensory properties (colour, odour, Brittleness, and general appearance) were investigated. In general, increasing the propolis extract concentration in samples leads to a reduction in the growth rate of the bacterial population, and it continued till the last day of storage at 4oC. The sensory study revealed that odour alterations in samples ...
Eman M. Abdelhady1, Nader Y. Mostafa1, Mohammed A. El-Magd2* and Ghada A. Kirrella1
...oparticles (CNPs) on the microbiological quality and shelf life of beef minced meat during its refrigerated storage for 9 days. The size of CNPs ranged from 150 to 350 nm as determined by transmission electron microscopy. Comparing the effect of the two chitosan products, CNPs exhibited higher antibacterial properties against S. aureus and E. coli, as revealed by lower minimal inhibition concentrations than the CP. Among these bacteria, E. coli was...
Nafissa Sahel1*, Fadela Chougrani2, Abderrahim Cheriguene3 and Zineb Hamani1
...the physico-chemical and microbiological characteristics of fresh camel milk collected from different regions of Southwestern Algeria. Samples were collected from 48 camels of El Bayadh, Bechar, Adrar and Tindouf. A wide variation was observed in the physico-chemical properties due to variation in feed, lactation period and environmental conditions. Microbiological results indicate that camel milk from Tindouf was the most c...
Muhammad Asif Iftikhar1, Talat Naseer Pasha2, Saima Inayat1, Khalid Javed3 and Rahman Ullah4*
...tural, compositional and microbiological properties of yoghurt. Sahiwal, Holstein Friesian milk procured from Dairy Animal Training and Research Center, B-block University of Veterinary and Animal Sciences, Pattoki and Nili Ravi buffalo milk procured from Nili Ravi buffalo Research Institute (BRI), Pattoki. All other ingredients purchased from Sigma Aldrich Germany through local suppliers. Milk was standardized to 15% total solids with 3.5% fat. Skim Milk Powd...
Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova
...ting the pathogen during microbiological cultivation. Therefore, the high correlation (r=0.76, P<0.05) between the results of express testing and the detection of the pathogen by bacteriological cultivation, as the main diagnostic method of emphysematous carbuncle, allows recommending its use if it is necessary to diagnose the disease in pasture conditions quickly.
Keywords | Express test, Polyclonal antibodies, Vaccine ...
Evy Rossi1*, Fajar Restuhadi1, Raswen Efendi1, Yusmarini1, Rahmayuni1, Emma Riftyan1, Bisma Panca Winata2, Yolanda Ashara2, Usman Pato1
Rafiullah1, Said Sajjad Ali Shah1*, Muhammad Ilyas Khan2, Anwar Ali3, Imtiaz Ali Shah1 and Sohaib ul Hassan4
Endy Triyannanto1, Danung Nur Adli2, Diah Pratiwi3, Lukman Hakim3, Selma Noor Permadi3, Taufik Kurniawan3, Angga Maulana Firmansyah3, Lina Ivanti3, Dinar Suksmayu Saputri3, Mohammad Miftakhus Sholikin4, Hari Hariadi5, Tri Ujilestari3, Teguh Wahyono3*
...nd protein peroxidation, microbiological aspects, and sensory characteristics aspects in pork. 52 experiments from 21 studies were compiled into an extensive database. The irradiation dose varied between 0 and 50 kGy during the meta-analysis. A mixed-model technique was applied to perform the meta-data analysis, with the gamma irradiation dose considered as fixed effects and the different research (articles) as random effects. Gamma irradiation reduced hardnes...
Afnan Ahmed Allhyani1, Mohamed Baeshen1, Nagwa Thabet Elsharawy2,3*
Marlon Reinaldo Castro-García1*,Vanessa Gabriela Espinoza-Posligua1, Francisco Horley Cañarte-García1, Doris Fernanda Rizzo-Alcivar2, Christian Simón Rivadeneira-Barcia1 and Alex Alberto Dueñas Rivadeneira3
...ity, film solubility and microbiological analysis. Results showed that the optimum concentration of gum for elaboration of films was 40% (v/v). Films have a good tensile strength showing values between 10.81 and 16.90 MPa. The elasticity showed values between 0.11 and 0.21 MPa. The water vapor permeability ranged between 0.87 - 4.32 x 10-10 g m-1 s-1 Pa-1, a solubility between 45.95 and 55.11% was obtained. The thick...
Eman Mamdouh Qenawy1, Mohamed Abou-Ellail1, Fatma Abdel-Motaal2, Mohammed O. Alshaharni3, Nady Khairy Elbarbary4*
...ty, Egypt, with distinct microbiological analysis that highlighted the detection of Pseudomonas aeruginosa and Escherichia coli. Ninety samples of meat in total (15 of each minced beef, sausage, burger, shawarma, and hawawshi) were obtained from several markets in Aswan City, Egypt, and exposed to bacterial analysis and PCR methods. The fraud samples exhibited a higher overall bacterial count than pure bovine meat. P. aeruginosa and E. coli were identified in ...
Abdussalam Muhyideen Zainab Funmilayo and Lee-Hoon Ho*
...e on the biochemical and microbiological quality of enzymatically pasteurized beetroot juice. A 3:1 ratio was used to blend the beetroot pulp with distilled water. After that, pectinase was added at a concentration of 1.5 U/mL for 4hrs and 40°C, and then clarified with pectinase and cellulase and their combination, at concentration of 1.0 U/ mL for 50°C and 15 min. After that, the mixtures were all kept at 90°C for five minutes in order to pasteuri...
Safaa El Bialy1*, Nabil Bker2, Hossam Ibrahim3, Saber, A. Saad4, Eman Ali5
...ng 3% PEO. Regarding the microbiological status, the results were dose-dependent, showing that higher concentrations of PEO, especially 3%, led to greater reduction percentages. The highest reduction, reaching 100%, was observed in the counts of Enterobacteriaceae and coliforms. The study concluded that using parsley essential oils at varying concentrations, particularly at 3%, could extend the shelf life of fish up to 8 days during refrigeration, specifically...
Hamayun Arshad1, Muhammad Waris2,3 and Qurratulann Afza Gardner1*
...vironment using standard microbiological techniques. Among those isolates, we found novel strain of L. sphaericus Q001, identified by ribotyping and MALDI-biotyping. The 16S ribosomal RNA gene of this strain is 96% similar to that of highly entomotoxic WHO reference strain 2362. The gene sequence was submitted to Genbank (OQ701385). We isolated the S-layer protein of this strain by treating it with lithium chloride (LiCl). After SDS-PAGE analysis, the protein ...
Aamara Ibrahim1, Ihsan Mabood Qazi1, Majid S. Hashmi1, Ayaz Ahmad1*, Hisham Javed1, Fawad Ahmad2 and Sadia Mukhtar1
... the physical, chemical, microbiological and taste aspects of cottage cheese. Different concentrations (%) of cow and goat milk were mixed such as (CG1 (100:0) served as control, CG2 (90:10), CG3 (80:20), CG4 (70:30), CG5 (60:40), CG6 (50:50) and CG7 (0:100)) to prepare milk blends. The analysis of physical and chemical properties showed notable differences in composition among the milk blends, with goat milk has higher fat content (4±0.003%) while cow ...
Ivania Farrah Nadhira1, Trioso Purnawarman2, Chaerul Basri2*
...re practices towards the microbiological quality of meat in traditional markets in Bogor City. This research uses survey method at all slaughter facilities in Bogor City, which all located at traditional markets. Animal welfare practice data was collected by observation using checklist in the Animal Welfare Development Guidelines Assessment Checklist for Animal Product Business Units 2023, while the microbiological quality o...
