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Syed Makhdoom Hussain1,*, Tasneem Hameed1, Muhammad Afzal2, Arshad Javid3, Nosheen Aslam4, Syed Zakir Hussain Shah6, Majid Hussain5, Muhammad Zubair ul Hassan Arsalan1 and Muhammad Mudassar Shahzad1
...stibilityof Cirrhinus mrigala fingerlings fed sunflower meal based diet. The chromic oxide was added as inert marker in the diets. Three replicate groups of 15 fish (Average weight 5.05±0.013 g fish-1) were fed at the rate of 5% of live wet weigh and feces were collected twice daily. The results of present study showed improved growth performance of Cirrhinus mrigala fingerlings in response to...

 Zeenat Bano1,*, Sajid Abdullah1, Waqas Ahmad2, Muhammad Anjum Zia3 and Wardah Hassan1

...ey) of fish, Cirrhina mrigala. The 3 different representative water samples and 60 fish samples (20 fish from each site) were collected from three sites i.e. fish-farm, hatchery and Indus River of Punjab, Pakistan. The results showed differences in physico-chemical parameters of water samples collected from all the sites. The concentrations of Zinc (Zn), copper (Cu) and nickel (Ni) were higher in river water as compared to farm and hatchery.
Huma Naz1,*, Sajid Abdullah2, Sidra Naz3, Sidra Abbas2, Wardah Hassan2, Shakeela Perveen2, Moazama Batool1, Laiba Shafique2
...or the fish, Cirrhina mrigala was determined in this research work. The fish were exposed to each of the 19 different concentrations (5-90 mg/L) of Fe+Zn+Pb+Ni mixture to determine 96 h LC50 and lethal concentration. During acute toxicity test, behavioral changes and catalase activity in liver, kidney, gills, brain and muscle of the fish were also studied. The mean 96 h LC50 and lethal value for C. mri

 Hamda Azmat1, Waqas Ali2, Arshad Javid2, Ali Hussain2, Syed Makhdoom Hussain3, Zara Saeed1,Syed Shafiq Hussain Bukhari4

Chromium contamination in water, sediment and its bioaccumulation in Indian major carps in River Chenab, Pakistan
...beo rohita and Cirrhinus mrigala was conducted from January through May, 2015. Water, sediment and fish samples were collected on monthly basis from upstream and downstream of head Trimmu, river Chenab. Throughout the study period, mmaximum Cr concentrations from upstream and downstream were recorded as 23.54± 3.43 mg/L and 21.12 ± 3.47 mg/L, respectively. Average metal concentration detected from sediment samples was 71.08±8.32 mg/L. Maxi...

Nasir Raza Zaidi1, Mian Waheed Ahmad1, Mahesh Gautam1, Riffat Mehboob2*

Risk factors for multiple sclerosis in Pakistani population- A crosssectional study

 Jhan Zeb, Muhammad Javed

Forecasting percentage contribution of plankton biomass towards increase in fish yield under composite culture conditions
...a, Catla catla, Cirrhina mrigala, Ctenopharyngodon idella and
Hypophthalmichthys molitrix in the ratio of 27:10:10:07:13, respectively. Each triplicate group of fish T1 to T6 were fed
supplementary diets at varying protein level viz., 22, 24, 26, 28, 30 and 32% digestible protein (DP), respectively @
2% of their wet body weight daily. However, control fish (T7) although had an access to plankton; but were devoid of
the availability ...

Farkhanda Asad1*, Samina Qamer1, Tayyaba Ali1, Ammara Behzad1 and Tahira Yasmin2 

...tinized corn in Cirrhinusmrigala fingerlings for 90 days. Using various levels of chromium chloride hexahydrate six test dietsdesignated as; T1(G/0.0, CrCl3.6H2O mg Kg-1), T2(NG/0.0, CrCl3.6H2O mg Kg-1), T3(G/0.2, CrCl3.6H2O mg Kg-1), T4(NG/0.2, CrCl3.6H2O mgKg-1), T5 (G/0.4, CrCl3.6H2O mg Kg-1) and T6(NG/0.4, CrCl3.6H2O mg Kg-1) were prepared. Results showed highest apparent digestibility coefficient (ADC) of nutrientsdry matter, crude lipid and gross energy ...
Fariha Latif* and Muhammad Javed
...hita and Cirrhina mrigala towards metals mixture (Cd+Cr+Cu+Pb) were determined during present research work. Sensitivity of the three fish species (120-day old) towards metals mixture was measured in term of 96 h LC50 and lethal concentration. These acute toxicity tests were performed at constant temperature (30oC), pH (8) and total hardness (250 mgL-1). All the three fish species varied significantly in their sensit...
Muhammad Ahmad, Zohaib Noor*, Karim Johar Khan, Waqar Younas and Ajmal Hussain
... feeder and Cirrhinus mrigala (C. mrigala) bottom feeder, respectively, while, in group 2 only (C. catla) was replaced with (Hypophthalmichthys molitrix) H. molitrix as a surface feeder keeping other species the same as in group 1. At the end of experiment H. molitrix showed considerably high weight gain (P< 0.05) in group 2 as compare to column and bottom feeder fish speci...
Shahid Sherzada1,2,4,*, Muhammad Naeem Khan4, Masroor Ellahi Babar2, Muhammad Idrees3, Abdul Wajid2, Muhammad Nauman Sharif3, Muhammad Shahzad Iqbal3, Iram Amin3 and Muhammad Shahid3
...>Labeo rohita, Cirrhinus mrigala, Catla catla)are very suitable fish species for culture in Pakistan. However, the identification and phylogeny of these fish species are of much interest today. DNA barcoding is used as a bio-identification tool for the organism globally. The current study was also conducted for the fast and accurate identification of Indian major carp species. The fishes used in this study were captured from four geographically isolated di...
Fariha Latif1*, Muhammad Javed2, Hammad Ahmad Khan2 and Khalil-Ur-Rehman3
...and brain of Cirrhina mrigala. The 120-days old fish were exposed to 1/4th (11.81mgL-1) and 2/3rd (31.49mgL-1) of 96-h LC50 of water-borne Pb for three fortnights (42 days) in glass aquaria at pH 7.5, temperature 30°C and total hardness 250mgL-1. After each fortnight, fish were sampled from each treatment and different organs such as liver, kidney, gills muscles and brain were taken o...

Hafiz Abdullah Shakir1*, Javed Iqbal Qazi2, Abdul Shakoor Chaudhry2, Muhammad Irfan3, Muhammad Khan1, Shaukat Ali4 and Saima Shahzad Mirza5 

...ita (rohu) and Cirrhinus mrigala (mori)) from river Ravi during two flow seasons at four sampling locations including upstream Lahore Siphon = A, Shahdera = B, Sunder=C and downstream Balloki headworks = D. All the metal contents in fish scales were highly significantly different (P<0.001) among sampling locations and flow seasons. Location-wise metal accumulation pattern was C > D > B > A. The highest concentrations (µg/g dry weight) of Cd (...
Daniel Cocan1, Vioara Mireşan1, Florentina Popescu1, Radu Constantinescu1, Aurelia Coroian1, Călin Laţiu1, Romulus Valeriu Flaviu Turcu3, Alexandru Ştefan Fărcăşanu3 and Cristian Martonos2,*

Zia-Ur-Rehman1, Faisal Sohail Fateh2*, Humayun Saleem1 and Muhammad Abu Bakar Siddique1 

...na Pul, Burma Chowk, Taramri, NIH Market, Chatha Bakhtawar, I-8, I-10/2, I-10 (Markaz), Sabzi Mandi, I-9. Maximum Mean Disease Index 77.78% was found in the market of Khanna Pul which supplies the fruits to high class, middle class and poor class families. But the study reveals the importance of post-harvest handling of the peach till it reaches to the consumers. 

...
Zarish Yaqoob1, Saleema Bashir Shams2, Gaitee Joshua2 and Bibi Nazia Murtaza2,3*
...0.1 ppm) in Cirrhinus mrigala and Zn (32.9 ppm) in Cyprinus carpio. Maximum accumulation of heavy metals was found in the gills of fish followed by liver, kidneys, muscle and fat cells. Overall, seventeen fish species were recorded in the study areas indicating a decrease in the number of species in Ravi. The results have shown that the water of Ravi is unsafe for aquatic life and irrigation. Metal concentration in the fish meat is higher than th...
Sana Khan1*, Faiza Aman1, Muhammad Ismaeel2, Zafar Ali2, Mehboob Alam1, Sadeed Iqbal1 and Taimur Khan3
 
...and cultivars (Meteor, Samrina Zard, Leena Pak and Pea-2009) to sub plots. Data was collected on growth and yield parameters of randomly selected plants in each treatment and replication. Results showed that phosphorus levels significantly affected all growth parameters. Maximum absolute growth rate (3.31cm day-1), leaflets plant-1 (102.61), pods plant-1 (16.43), pod length (9.53 cm), root weight (4.67 g), nodules plant-1 ...

Saima Naz1*, Ahmad Manan Mustafa Chatha2, Saba Saeed1

...beo rohita and Cirrhinus mrigala was determined in this study. During metal stress trials both species were kept at constant temperature (32°C), pH (7) and hardness (250 mg L-1) of water. Physico-chemical parameters of test medium were monitored regularly. The observed mean LC50 and lethal concentrations for Cirrhinus mrigala were 55.85 ± 2.84 and 128.44 ± 9.25 mg L-1, respectively. On the other hand, for L...
Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1
...tious to Salmonella typhimrium ATCC25566 and S.
typhimurium MM11 among the eight bacterial isolates tested belonging to family
enterobacteriacae. The thermal inactivation point of the isolated phage was found to
be 76 ºC. Salmonella bacteriophage (St-1) survived for 7 days at 4, 25, 37, 42 and -
20 ºC. The virus lost its ability to lyse salmonella cells at pH 4, 5, 6, 10, 11 and 12
while it ...
Syed Sikandar Habib1*, Saira Naz2, Sadia Nawaz1, Iqra Ameer1, Ameer Khatoon1, Hameed Ur Rehman3, Sahibzada Muhammad Jawad4 and Haider Ali5
...>Labeo rohita, Cirrhinus mrigala, Cyprinus carpio, Hypophthalmichthys molitrix, Ctenopharyngodon idella). Fish species selected for the current study is generally widely cultured in Pakistan and worldwide. Fish sampling was done by using different nets (river and farm) and blood sampling was immediately drawn from caudal peduncle by syringe and added in EDTA containing vial and shake well for proper mixing. Vials stored in ice containing boxes and carried ...

Kashifa Naghma Waheed*, Tariq Rashid, Sikender Hayat, Muhammad Azeem and Sajid Ali Naqvi

...beo rohita and Cirrhinus mrigala during twelve-week period. The data obtained was subjected to Multivariate analysis of variance (MANOVA) to find out the statistically significant difference (p<0.01) between two treatments for their response to fish growth. The post-hoc test revealed that growth of Major carps was significantly higher with greenhouse as compared to without greenhouse (at p < 0.05). Growth and survival rate of Catla catla were found more ...

Nisar Ahmad1, Syed Makhdoom Hussain2,*, Azhar Rasul2, M. Mudassar Shahzad3, Arshad Javid4, Hamda Azmat5, M. Zubair ul Hassan Arsalan2, Sadia Tabassum2 and Bilal Ahmad2

...arcass composition of C. mrigala fingerlings fed nano-Se particles supplemented sunflower meal based diets. The experiment was consisted on seven test diets on the basis of supplementation of nano Se graded levels (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg/kg). Chromic oxide was added as an inert marker. Fingerlings were fed at the rate of 5% of their live wet weight. Maximum improvement in mineral absorption (Ca, Na, K, Cu, P and Al) was observed at test diet with 1.5 ...

Shahid Sherzada1,2*, Muhammad Naeem Khan1 and Masroor Ellahi Babar2

...analysis of by Cirrhinus mrigala using mitochondrial DNA marker cytochrome oxidase I (COI). Genomic DNA isolated from whole blood was used to amplify and sequence a short region of COI gene in mitochondrial DNA. The identification of sequenced samples was done by NCBI (98-99%) and BOLD (99%) databases. The sequence data analysis represented 15 variable polymorphic sites and 4 haplotypes. Mean haplotypes and nucleotide diversity was 0.42 and 0.0018, respectivel...

Majid Hussain1, Syed Makhdoom Hussain2, Razia Iqbal3, M. Mudassar Shahzad4,*, Syed Zakir Hussain Shah3, Afia Muhammad Akram4, Nisar Ahmad5 and Muhammad Zubair ul Hassan Arsalan2

...cal indices in Cirrhinus mrigala fingerlings. Basal diet was supplemented with 0%, 1%, 2%, 3%, 4% and 5% CA resulting in the formulation of six experimental diets. Ten fingerlings were stocked in tanks in triplicate for each treatment. Feed was given at 5% live wet body weight of fingerlings for 90 days. Results showed that diet acidification with CA significantly (p< 0.05) improved the mineral absorption, carcass composition and hematological indices of C....
Saeed Akram Khan1, Shahid Sherzada1*, Muhammad Ashraf1, Durr-e-Shehwar1, Sonia Iqbal1, Usman Atique1,3, Farzana Abbas1, Sohail Ahmad2 and Muhammad Sultan Haider4
...ign: justify;">Cirrhinus mrigala naturally breeds in rivers and streams during monsoon season but in ahatchery, it  can be induced to spawn by the administration of ovaprim or its analogues. However, the conditions of the induced environment vary from place to place as well as the dose of ovaprim. The present investigation is focused on possible impact of different temperatures on the induced spawning behavior of C. mri...
Neeranoot Detcharoenyos1, Nakrob Pattanapon1, Soontaree Petchdee2*

Hira Jabbin1, Muhammad Arshad1*, Naunain Mehmood1, Wardah Hassan1, Syed Zakir Hussain Shah2 and Farzana Siddique3

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1 

...me Thaila) and Cirrhinus mrigala (CM; local name Morakhi). The samples were processed for isolation of bacterial organisms using conventional culture method and isolates were tested for antimicrobial sensitivity according to CLSI method. According to results, the prevalence of bacterial organisms was high (p < 0.05) in CC than CM and LR. E.coli were the most common organism in market and pond fish samples with occurrence of 86.6% and 66.6% respectively. Nex...
Durairaja Ramulu1*, Jawahar Paulraj2, Jayakumar Natarajan1, Sudhan Chandran1 Sudhir Kumar Das3, Suresh Eswaran4, Santhakumar Rajagopal5 and Padmavathy Pandurangan6
...ers of Mirgal, Cirrhinus mrigala (Hamiltoon, 1832) (L∞ 89.25 cm, K 0.8y-1), Blackspot barb, Dawkinsia filamentosa (Valenciennes, 1844) (L∞ 22.05 cm, K 0.68y-1) and Roho labeo, Labeo rohita (Hamilton, 1822) (L∞ 47.25 cm, K 0.71y-1) and the mortality parameters of C.mrigala (Z 2.14 y-1, M 1.22 y-1 and F 0.92 y-1), D. filamentosa (Z 2.42 y-1, M 1.54 y-1 and F 0.88 y-1) and L. rohita (Z 2.87 y-1, M 1.42 y-1 and...

Wenxiao Jia1*, Yilinuer Yilihamu1, Yunling Wang1, Shuang Ding1, Dilinuerkezi Aihemaiti1, Hanjiaerbieke Kukun1 and Yanhui Ning2

Muhammad Adnan Khalid1, Syed Makhdoom Hussain1*, Shafaqat Ali2,3*, Abdullah Ijaz Hussain4, Muhammad Asrar1, Nisar Ahamd5, Majid Hussain6 and Muhammad Zubair-ul-Hassan Arsalan7

...nt activity of Cirrhinus mrigala. At the conclusion of feeding trial, the protein content was highest (18.51±0.13%) in fingerlings fed with poultry waste biochar and lowest (15.35±0.11%) in fish fed with parthenium biochar. Mineral content was also significantly different in each fish group as compared to control diet. The diet contained Parthenium biochar showed poor results (P<0.05) in terms of minerals except Se (0.65±0.004 mg/kg). A...

Syed Saqlain Hussain1*, Muhammad Rasheed2, Zammurad Iqbal Ahmed2, Ghulam Jilani3, Muhammad Irfan4, Muhammad Kashif Aziz5, Fiaz Hussain1, Muhammad Akhlaq Mudassar1 and Zuhair Hasnain2

Hafiz Muhammad Ashraf, Hafiz Abdullah Shakir* and Javed Iqbal Qazi

...h flow season, Cirrhinus mrigala and Captodon zilli showed highest relative abundance 13.89% and 13.73%, respectively. Highest frequency occurrence (FO) 87.5% was observed for Labeo rohita followed by two species Channa punctate and Cirrhinus mrigala (75%). Maximum Shannon-Weiner index 3.119 and 3.122, Margalef index 2.825 and 3.573, Evenness index 0.9728 and 0.9849 were recorded during low and high flow seasons, respectivel...

A 90-day feeding trial was designated to evaluate how canola meal (CM)-based diet supplemented with phytase (PHY) and citric acid (CA) affects whole-body composition and hematological parameters in Cirrhinus mrigala and Cyprinus carpio fingerlings. Sixteen experimental diets (T1─T16) with varied CA (0 and 2.5%) and PHY (0 and 750 FTU/kg) levels were prepared. The fingerlings were fed at the rate of 5.0% of live fish body mass. Fifteen fish were randomly stocked into triplicate tanks for each of sixteen test diets. Significant (p<0.05) increase in crude protein (CP) and crude fat (CF) was noticed in fish fed T12 (2.5% CA and 750 FTU/kg PHY) diets. Comparison of treatments showed maximum values of RBCs (3.57×106 mm-3 and 3.18×106 mm-3), PLT (83.32 and 80.35), Hb (8.92g/100ml and 8.62g/100ml) and PCV (29.07% and 30.07%) in C. mrigala and C. carpio fingerlings, respectively, when fed with T12 diets. Conclusively, a 50% CM-based diet with 2.5% CA and 750 FTU/kg PHY supplementation, performed better in terms of whole-body composition and hematological parameters in C. mrigala and C. carpio fingerlings.

... parameters in Cirrhinus mrigala and Cyprinus carpio fingerlings. Sixteen experimental diets (T1─T16) with varied CA (0 and 2.5%) and PHY (0 and 750 FTU/kg) levels were prepared. The fingerlings were fed at the rate of 5.0% of live fish body mass. Fifteen fish were randomly stocked into triplicate tanks for each of sixteen test diets. Significant (p<0.05) increase in crude protein (CP) and crude fat (CF) was noticed in fish fed T12 (2.5% CA and 750 FTU/kg...

Nesreen Ahmed Nasser1, Anas H. Sadek2, Bayadir Abdulhussein Mahmeed3, Osama Akram Mohsein4* 

South Asian Journal of Life Sciences

December

S. Asian J. Life Sci., Vol. 12

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