Issue · 2019
Vol. 51, Iss. 4, Pages 1203-1598
48 articles · pp. 1203-1598 · All issues →
Short CommunicationA Homozygous c.1131G>A Missense Mutation in BBS9 Gene Manifesting Autosomal Recessive Bardet-Biedl Syndrome in Consanguineous Kashmiri Family
Syeda Ain-ul-Batool, Sadia, Kathrin Blasius, Angela Kaindl, Ghazanfar Ali
Bardet-Biedl syndrome (BBS) is a rare autosomal recessive ciliopathic genetic disorder in humans. It is a multisystem disorder and is principally described by visual abnormalities, con-rod dystrophy, eyes exotropia, obesity, polydactyly, hypogonadism, and rena…
3 Jun 2019DOI 10.17582/journal.pjz/2019.51.4.sc1
Short CommunicationEffect of Di-n-butyl Pthalate on Oxidative Stress Parameters in Liver and Gills of Labeo rohita
Iqra Jaffar, Zainab Sehzadi, Muhammad Adeel, Khalid P. Lone, Mehwish Faheem
Phthalates are plasticizers, released from different sources, are ubiquitous in the aquatic environment. In the present study, freshwater fish (Labeo rohita) juveniles were exposed to low concentrations (10μg/l and 100μg/l) of di-n-butyl phthalate (DBP)…
5 Jun 2019DOI 10.17582/journal.pjz/2019.51.4.sc6
Short CommunicationAlbendazole in the Treatment of Ancylostomiasis, Ascariasis, Taeniasis and Amoebiasis in School Children
Wali Khan, Mudassar Iqbal, Israr Khan
Present study was aimed to evaluate the efficacies of two brands of albendazole: bendazol and zentel against ancylostomiasis, ascariasis, taeniasis and amoebiasis in school children to make appropriate treatment protocol. All the infected children were randoml…
5 Jun 2019DOI 10.17582/journal.pjz/2019.51.4.sc4
Short CommunicationRe-Description and Illustration of Two Species of the Genus Parabaliothrips Priesner, 1935 (Thysanoptera: Thripidae), with Report of New Host Plant
Qingling Hu
Two species of the genus Parabaliothrips Priesner 1935 (P. coluckus and P. takahashii)are re-described and illustrated in detail based on specimens collected from South China. A checklist of the known species in this genus is provided. New host plant of the ge…
4 Jun 2019DOI 10.17582/journal.pjz/2019.51.4.sc3- Research Article
Effects of Mulberry Leaves with Bacillus subtilis on Growth and Production Performance of Silkworm, Bombyx mori L.
Wang Zaigui, Guo Panpan, Ye Miao, Sun Linghong, Zhang Hongfu, Liu Chaoliang
The study was conducted with 1500 healthy silkworm larvae. There were two treatment groups: control group, without added Bacillus subtilis, and B.subtilis group, with B. subtilis at 2.0×1010 CFU/mL added to the feed. Experimental diets were fed in three…
29 May 2019DOI 10.17582/journal.pjz/2019.51.4.1555.1560 
Research ArticleDistribution, Dietary Breadth and Niche Overlap between Two Sympatric Mongoose Species Inhabiting Pir Lasura National Park, Azad Jammu and Kashmir, Pakistan
Faraz Akrim, Tariq Mahmood, Muhammad Sajid Nadeem, Siddiqa Qasim, Shaista Andleeb, Hira Fatima
Knowledge of a predator’s diet and distribution is vital for its conservation and management. We investigated dietary breadth of two carnivore species; Indian grey mongoose (Herpestes edwardsii) and small Indian mongoose (Herpestes javanicus) occurring i…
18 May 2019DOI 10.17582/journal.pjz/2019.51.4.1497.1507
Research ArticleInvestigation on Oxygen and Carbon Dioxide Fluctuations in Lasiopodomys mandarinus Burrows
Sun Hong, Zhang Yifeng, Wang Baishi, Li Yangwei, Xu Wenbo, Mao Runkun et al.
The mandarin voles (Lasiopodomys mandarinus) is a subterranean rodent that spends its entire life underground in burrow systems, having to endure a hypoxic environment. In this study, we used the embedded artificial tunnel method in a natural burrow system to…
18 May 2019DOI 10.17582/journal.pjz/2019.51.4.1519.1526
Research ArticleResponse of Liver-Type Fatty Acid-Binding Protein (L-FABP) Gene in Golden Pompano Trachinotus ovatus (Linnaeus 1758) to Temperature and Nutrient Manipulations
Shengjie Zhou, Pengfei Wang, Chao Zhao, Mingjun Fu, Jian G. Qin, Lihua Qiu et al.
The liver-type fatty acid binding proteins (L-FABP) gene in golden pompano Trachinotus ovatus larvae was cloned in the present study. The full length of L-FABP cDNA from golden pompano was 604 bp, including a 5’-untranslated region (UTR) of 154 bp, a 3&r…
15 May 2019DOI 10.17582/journal.pjz/2019.51.4.1465.1473
Research ArticleComparative Study on the Nymphal Stages of Two Sphingonotus Species (Acrididae: Oedipodinae)
Fakhra Soomro, Riffat Sultana, Muhammad Saeed Wagan
Nymphal stages of two species of genus Sphingonotus Fieber viz. S. savignyi Saussure and S. rubescens rubescens (Walker) were studied exclusively form Sindh during the 2014-2016. Both species passed through six instars larvae. Emergence of larval instars in bo…
3 May 2019DOI ttp://dx.doi.org/10.17582/journal.pjz/2019.51.4.1379.1386
Research ArticleMiddle Miocene Suids from Chinji Formation of Chabbar Syedan, Punjab, Pakistan
Muhammad Khalil Nawaz, Sayyed Ghyour Abbas, Muhammad Akbar Khan, M. Adeeb Babar, Muhammad Asim, Rabia Shahid et al.
Listriodon pentapotamiae, Conohyus sindiensis and Propotamchoerus sp. described in this article, have been recovered from a long neglected Middle Miocene locality of Chabbar Syedan, Jhelum district, Punjab, Pakistan. The lower deciduous incisor (di1) of L. pen…
3 May 2019DOI 10.17582/journal.pjz/2019.51.4.1343.1353
Research ArticleOptimization of Conditions for Maximal Production of Recombinant Thermostable Cellulase from Thermotoga naphthophila using E. coli BL21-CodonPlus (DE3) as Expression Host
Aisha Khalid, Muhammad Tayyab, Abu Saeed Hashmi, Tahir Yaqub, Ali Raza Awan, Muhammad Wasim et al.
Current study was designed for the development of an economic and environment friendly mechanism for the production of thermostable cellulase. Production of cellulase was focused due to its diverse range of application in industry. In the present study, condit…
3 May 2019DOI 10.17582/journal.pjz/2019.51.4.1371.1377
Research ArticleEffect of Different Temperatures on Colony Characteristics of Bombus terrestris (Hymenoptera: Apidae)
Muhammad Nasir, Ata-ul-Mohsan, Munir Ahmad, Shafqat Saeed, Muhammad Asif Aziz, Muhammad Imran et al.
In the present study, effect of five different temperatures (24 ºC, 26 ºC, 28 ºC, 30 ºC and 32 ºC) was studies on different colony characteristic of post-hibernated queens of Bombus terrestris during flight activity period and solitary…
2 May 2019DOI 10.17582/journal.pjz/2019.51.4.1315.1322
Research ArticleAnalyzing Stopover and Wintering Habitats of Hooded Cranes (Grus monacha): Implications for Conservation and Species Dispersion in the East Asia
Tianlong Cai, Falk Huettmann, Kisup Lee, Yumin Guo
The hooded crane (Grus monacha) is a vulnerable species. However, its stopover habitat receives little attention and is not well known or protected even. Here, we present the spatial distribution of the stopover habitats for hooded cranes in the East Asia. A m…
2 May 2019DOI 10.17582/journal.pjz/2019.51.4.1323.1333
Research ArticleAnalysis of the Phylogeny and Evolutionary Selection Pressure of the Mx Gene in 10 Wild Birds
SiRui Wang, Fekede Regasa Joka, XiaoLong Wang, SuYing Bai
The Myxovirus-resistance (Mx) gene has broad-spectrum antiviral effects. This gene is particularly important for the prevention and treatment of avian influenza. Currently, this gene is more studied in poultry, and studies in wild birds are rare. To understand…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1299.1307
Research ArticleEffect of Different Diapause Conditions on Bombus terrestris Colony Characteristics
Muhammad Nasir, Ata-ul-Mohsan, Shafqat Saeed, Munir Ahmad, Muhammad Asif Aziz2 andMunir Ahmad
Diapause is the very important stage in the artificial rearing of bumblebees that entails costs that become visible after diapause completion. In the present study different diapause temperature (-2-0 ºC), (1-3 ºC), (4-6 ºC) and duration (two an…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1273.1280- Research Article
Effect of High Vitamin E Dosages on Lipid Peroxidation and Fatty Acid Profile of Labeo rohita Fingerlings
Mahroze Fatima, Muhammad Afzal, Syed Zakir Hussain Shah
A 60 days feeding trial was conducted to investigate the effect of dietary high doses of vitamin E on Labeo rohita fingerlings. Five experimental diets were formulated to contain one control (VE0), two adequate levels of vitamin E supplementation, i.e. 1…
29 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1211.1219 
Research ArticleMorphology of Immature Stages of Schistocerca gregaria with Special References to its Size Variations
Ahmed Ali Samejo, Riffat Sultana
Adults as well as immature of Schistocerca gregaria are voracious vegetation consumer and destructive pest to the useful plants. Morphology and morphometry of immatures of desert locust in relation to gaining of body length and weight at each consecutive stage…
29 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1221.1226
Research ArticleGenetic Diversity among some Horse Breeds in Pakistan
Faiz-ul Hassan, Muhammad Sajjad Khan, Muhammad Saif-ur-Rehman, Syed Muhammad Raihan Dilshad, Muhammad Amjad Ali
Genetic diversity among 12 horse breeds present in Pakistan was determined by using RAPD markers. The blood samples of purebred animals of eight exotic breeds (Thoroughbred, Arab, Anglo-Arab, Suffolk, Percheron, Clevland, Noriker and Hanovarian) present…
28 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1203.1209
Research ArticlePossible Antiproliferative Effect of Tolfenamic Acid on Human LNCaP Prostate Cancer Cell Lines
Rıfat Ertekin, Mete Ozkurt, Gokhan Kus, Selda Kabadere
Prostate cancer is a common type of cancer. Contemporary treatment modalities require that new, effective methods or chemicals should be developed. Tolfenamic acid (TA) is a nonsteroidal anti-inflammatory medicine stimulating apoptosis in cancer cells like pan…
7 May 2019DOI 10.17582/journal.pjz/2019.51.4.1413.1419
Research ArticleMolecular Characterization of Codling Moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) in Swat Valley Pakistan using Randomly Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction
Hayat Zada, Ahmad-Ur-Rahman Saljoqi, Ijaz Ali, Bashir Ahmad, Abdul Waheed Khan, Shabeer Ahmad
Codling moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) is the most important insect pest of apple production in Pakistan. Genomic DNA was extracted from 30 overwintering larvae of each population of Matta, Madyan and Kalam regions. Out of 30 tested…
29 May 2019DOI 10.17582/journal.pjz/2019.51.4.1547.1554
Short CommunicationUltrastructural Characterization of Meloidogyne graminis from Golf Course Turf Grasses in Peninsular Malaysia
Shamsudin Bojang, Idris Abd Ghani, Jugah Kadir, Adamu Saidu Paiko, Yasir Iftikhar, Muhammad Kamran
During year 2015, a survey was conducted in 18 golf course greens from nine states (Selangor, Negeri Sembilan, Melaka, Johor, Pahang, Perak, Kedah, Pulau Pinang and Wilayah Persekutuan) of Peninsular Malaysia for nematode, Meloidogyne graminis infections on th…
5 Jun 2019DOI 10.17582/journal.pjz/2019.51.4.sc5
Research ArticlePopulation Genetic Structure and Genetic Diversity of Coral Reef Species Lethrinus olivaceus in the South China Sea
Zhaochao Deng, Na Song, Yongzhen Li, Tianxiang Gao, Zhiqiang Han
Lethrinus olivaceus is an important coral reef fish in the South China Sea. To reveal the genetic structure and genetic diversity of L. olivaceus, a 495 bp segment of mitochondrial DNA control region was sequenced. We collected a total of 65 individuals from t…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1289.1297
Research ArticleCharacterization of Thermophilic Bacteria Anoxybacillus rupiensis and Cultivation in Agroindustrial Wastes Isolated from Hot Spring in Chakwal, Pakistan
Faiza Jabeen, Bushra Muneer, Javed Iqbal Qazi
Two thermophilic bacteria were isolated from hot spring located near the city Chakwal in Pakistan. The cells of both strains were rod-shaped, stained Gram positive and formed endospores. The isolates were able to utilize sugars like fructose, Maltose, mannose,…
30 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1243.1250
Research ArticleConformational Changes in Wild Type KRAS Induced by Two Novel Variants p.E31K and p.G138V
Bibi Nazia Murtaza, Azhar Qayum, Shamaila Inayat Nadeem, Naif Awdh Al-Maliki, Abdulaziz Alamri, Abdul Rauf Shakoori
Ras proto-oncogene encodes for small GTPases, downstream of epidermal growth factor receptor (EGFR) in the RAS/RAF/MAPK pathway. Wild type KRAS is associated with EGFR-signalling activation. In normal physiological conditions, it is activated by upstream signa…
29 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1227.1233
Research ArticleHouttuynia cordate Thunb Boosts the Non-Specific Immune Response and Enhances Resistance to Edwardsiellosis in the Olive Flounder (Paralichthys olivaceus)
Fan Yi, Xiaobin Yang, Shigen Ye, Hua Li, Ruijun Li
Houttuynia cordata Thunb (HCT) has been widely used as traditional Chinese medicine in human and livestock for its antiviral and antibacterial activities. In this study we are investigating the effect of HCT in aquaculture animals. In this study, 300 olive flo…
3 May 2019DOI 10.17582/journal.pjz/2019.51.4.1363.1369- Research Article
Behavioral Responses of Coccinella septempunctata and Diaeretiella rapae under the Influence of Semiochemicals and Plant Extract in Four Arm Olfactometer
Bushra Siddique, Muhammad Tariq, Muhammad Naeem, Muhammad Ali
Natural enemies are more effective at controlling herbivores in diverse botanical ecosystems. Different chemical cues help to correspond in diversity of associations between prey and host plant species. Recent studies exhibited that the use of natural enemy is…
7 May 2019DOI 10.17582/journal.pjz/2019.51.4.1403.1411 - Research Article
Antimicrobial Susceptibilities of Commensal Escherichia coli Isolates Recovered from Cloacal Samples of Japanase Quails and Chickens in Turkey
Emel Banu Buyukunal, Rojan Ibrahim Albazaz, Mehmet Ali Bal
Antibiotic resistance has been rapidly increasing all over the world. Presence of antibiotic resistant bacteria in food producing animals is considered an important transmission source for both direct and indirect transfer of those bacteria to human. For this…
13 May 2019DOI 10.17582/journal.pjz/2019.51.4.1439.1445 
Research ArticleEffect of Stocking Density on Growth, Serum Biochemical Parameters, Digestive Enzymes Activity and Antioxidant Status of Largemouth Bass, Micropterus salmoides
Yuyu Wang, Gangchun Xu, Zhijuan Nie, Quanjie Li, Nailin Shao, Pao Xu
A 150 day feeding trial was performed to investigate the effect of stocking densities on growth performance, serum biochemical parameters, digestive enzymes activity and antioxidant status of largemouth bass (Micropterus salmoides) reared in an in-pond raceway…
18 May 2019DOI 10.17582/journal.pjz/2019.51.4.1509.1517
Research ArticleComparing Predictive Performances of Tree-Based Data Mining Algorithms and MARS Algorithm in the Prediction of Live Body Weight from Body Traits in Pakistan Goats
Senol Celik
The main purpose of this investigation was to comparatively evaluate predictive performances of multivariate adaptive regression splines (MARS), chi-squared automatic interaction detector (CHAID), exhaustive CHAID and classification and regression trees (CART)…
15 May 2019DOI 10.17582/journal.pjz/2019.51.4.1447.1456
Research ArticleExtended-Spectrum-β-Lactamase Producing Multidrug Resistant Klebsiella pneumoniae Isolates from Pediatrics
Sumreen Hayat, Muhammad Hussnain Siddique, Bilal Aslam, Habibullah Nadeem, Asma Ashraf, Muhammad Saqalein et al.
Klebsiella pneumoniae is mainly responsible for nosocomial and urinary tract infections (UTIs) in pediatric and adult population. The ESBL producing K. pneumoniae present an alarming situation for clinicians by limiting the treatment options. We aimed to deter…
30 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1251.1257
Research ArticleHabitat Manipulation through Intercropping for the Management of Codling Moth Cydia pomonella (Linnaeus) (Lepidoptera: Tortricidae) in Swat Pakistan
Hayat Zada, Ahmad-ur-Rahman Saljoqi
Habitat manipulation through intercropping (Brassica campestris, Brassacicacae), Glycine max, leguminacae), Trifolium alexandrinum, Fabaceae) and Triticum aestivum, Poaceae) in the apple orchard were a substantial effect on the management of C. pomonella (L).…
29 May 2019DOI 10.17582/journal.pjz/2019.51.4.1537.1545
Research ArticleIsolation and Biological Characterization of Muscle-Derived Stem Cells from Sheep Skeletal Muscle
Ping Zhang, Yabin Pu, Yu Zhang, Jia Chen, Kunfu Wang, Qian Li et al.
The Objective of this study was to investigate the isolation, cultivation, identification and differentiation potential of Muscle-derived stem cells (MDSCs) from sheep skeletal muscle and provide an experimental evidence for clinical application. The skeletal…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1259.1272
Research ArticleNew and Known Nematodes Associated with Cotton Plantation in Sindh, Pakistan
Ashfaque Ahmed Nahiyoon, Shahina Fayyaz, Nasira Kazi
Cotton is a major crop of Pakistan after wheat and it occupies the largest area in Pakistan compared to other crops. Cotton is cultivated in Sindh on more than 1 million acres. Two popular varieties of cotton are grown in Sindh. The aim of the present study wa…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1309.1314
Research ArticleStudies on the Recombinant Production in E. coli and Characterization of Pharmaceutically Important Thermostable L-Asparaginase from Geobacillus thermodenitrificans
Muhammad Shahid Nadeem, Maryam A. Al-Ghamdi, Jalaluddin Azam Khan
L-asparaginase is ubiquitously found in microbes, animals and plants. The asparaginase of bacterial origin has been extensively applied in the treatment of childhood lymphomas and leukaemia. However, the glutaminase activity of enzyme can cause serious side-ef…
30 Apr 2019DOI 10.17582/journal.pjz/2019.51.4.1235.1241
Research ArticleDevelopment and Validation of a Transcriptome-Based Simple Sequence Repeats Markers in Coilia nasus
Fukuan Du, Yongkai Tang, Juhua Yu, Shengyan Su, Fan Yu, Jianlin Li et al.
Estuarine tapertail anchovy (Coilia nasus) is a commercially important species. However, eco-environment deterioration and overfishing have almost caused extinction of the species in the Yangtze River. Therefore, it is very urgent to perform genetic research o…
2 May 2019DOI 10.17582/journal.pjz/2019.51.4.1335.1341- Research Article
Toxic Effect of Insecticides Mixtures on Antioxidant Enzymes in Different Organs of Fish, Labeo rohita
Huma Naz, Sajid Abdullah, Khalid Abbas, Wardah Hassan, Moazama Batool, Shakeela Perveen et al.
In this study insecticides toxic effects of mixture of organochlorine (endosulfan) and organophosphate (chlorpyrifos) investigated on the activity of antioxidant enzymes viz. superoxide dismutase, peroxidase, catalase and glutathione S-transferase in different…
3 May 2019DOI 10.17582/journal.pjz/2019.51.4.1355.1361 
Research ArticlePurification and Characterization of an Acidic Polygalacturonase from Grapes and its Potential to Improve Juice Quality
Zahra Nazir, Saba Ijaz, Roquyya Gul, Mahjabeen Saleem
Pectinolytic enzymes, widespread in plants, fungi and microorganism, gain commercial importance for improving the yield and nutraceutical properties of juice processing industry, degumming of fibre plants and maximum oil recovery. These enzymes break down comp…
6 May 2019DOI 10.17582/journal.pjz/2019.51.4.1387.1402
Research ArticleAero and Food Allergens Sensitization Patterns in a Clinic-Based Sample in Pakistan: A One Year Retrospective Study
Abid Hussain, Farida Ahmed, Shyamali C. Dharmage, Shafiq ur Rehman, Muhammad Shahbaz Aslam, Zaigham Abbas
Sensitization to different allergens may lead to chronic clinical conditions like asthma and allergic rhinitis. Present study was conducted to investigate the prevalence of food and aero-allergen sensitization patterns. This retrospective study was designed to…
12 May 2019DOI 10.17582/journal.pjz/2019.51.4.1429.1437
Research ArticleNonlinear Growth Functions for Body Weight of Thalli Sheep using Bayesian Inference
Farhat Iqbal, Abdul Waheed, Zil-e-Huma, Asim Faraz
This article deals with the Bayesian approach for estimating the parameters of nonlinear growth functions used for modelling the body weight of farm animals. Gompertz, Brody, Von Bertalanffy and Logistic growth models were employed on the basis of nonlinear le…
12 May 2019DOI 10.17582/journal.pjz/2019.51.4.1421.1428
Research ArticleThe mRNA Expression of Host Defense Peptides in Chicken Erythrocytes are Highly Related to Tibial Dyschondroplasia and Induced by Recombinant Glutathione-S-Transferase A3 Protein
Sheng Niu, Ali-Raza Jahejo, Fa-jie Jia, Xin Li, Guan-bao Ning, Ding Zhang et al.
Chicken erythrocyte, which is the most abundant cell subset in blood vessel, also exists in cartilage zone. It has recently been shown that chicken erythrocytes constitutively express toll-like receptors (TLRs), cytokines, and avian β-defensins (AvBDs). H…
18 May 2019DOI 10.17582/journal.pjz/2019.51.4.1475.1482
Research ArticleEffects of Five Pesticides on Toxicity, Detoxifying and Protective Enzymes in Phauda flammans Walker (Lepidoptera: Zygaenidae)
Ailing Huang, Lingyu Meng, Wei Zhang, Junyan Liu, Guangyao Li, Huihua Tan et al.
Effects of beta-cypermethrin, abamectin, chlorpyrifos, thiamethoxam and bisultap on toxicity against 1st-6th instar larvae in Phauda flammans Walker (Lepidoptera: Zygaenidae) were tested using the leaf-dipping method. Meanwhile, effects of these pesticides on…
15 May 2019DOI 10.17582/journal.pjz/2019.51.4.1457.1463- Research Article
Comparison of Growth, Proximate and Minerals Composition of Commonly Polycultured Fish Species by Replacing Surface Feeder Fish
Muhammad Ahmad, Zohaib Noor, Karim Johar Khan, Waqar Younas, Ajmal Hussain
The experiment was conducted in the earthen ponds as two experimental groups were set each stocked with three different fish species at the rate of 800/Acre in the semi intensive polyculture system by supplementing inorganic and organic fertilizers in the pond…
18 May 2019DOI 10.17582/journal.pjz/2019.51.4.1483.1487 
Research ArticleHydrolyzed Chicken Feather Meal as Protein Source for Red Tilapia (Oreochromis sp.) Aquafeeds
Nursinatrio, Rudy Agung Nugroho
An 84-day feeding trial was conducted to determine the effects of Hydrolyzed Chicken Feather Meal (HCFM) supplementation on the growth, feed efficiency, survival rate and carcass composition of red tilapia (Oreochromis sp). A group of red tilapias (initial wei…
18 May 2019DOI 10.17582/journal.pjz/2019.51.4.1489.1496
Research ArticlePopulation Genetic Structure of Nuchequula mannusella (Perciformes: Leiognathidae) Population in the Southern Coast of China Inferred from Complete Sequence of mtDNA Cytb Gene
Bingbing Gao, Na Song, Zhonglu Li, Tianxiang Gao, Liqin Liu
Population genetics of marine fishes is vitally important in fishery management and conservation. The ponyfish species Nuchequula mannusella is a dominant species along southern coast of China with important economic and ecological value, and little of its pop…
28 May 2019DOI 10.17582/journal.pjz/2019.51.4.1527.1535
Short CommunicationIdentification of Mutations in Gene BRCA1/2 in Breast Cancer Cases from Balochistan, Pakistan
Asma Yousafzai, Muhammad Luqman, Nisar Ahmed, Muneeza Arbab, Muhammad Murad, Naheed Sajjad et al.
BRCA1/2 genes are highly susceptible genes for both breast and ovarian cancer and account for 15-20% of all the hereditary breast cancer cases. Present study was aimed at identifying mutation in BRCA1/2 in breast cancer patients in Balochistan. Blood samples o…
3 Jun 2019DOI 10.17582/journal.pjz/2019.51.4.sc2
Research ArticleReliable Scarless Fusion of Genes through Overlap Extension PCR to Develop Chimeric Genes
Iram Gull, Muhammad Shahbaz Aslam, Imran Tipu, Roohi Mushtaq, Muhammad Amin Athar
Overlap extension PCR is used to make versatile chimeric gene products. However, the results of this technique are not always reliable due to inefficient overlapping of the sequences especially those derived from authenticated native sequences. Certain forbidd…
3 Jun 2019DOI 10.17582/journal.pjz/2019.51.4.1561.1567
Research ArticleDistribution of Phenotypic and Genotypic Antibiotic Resistance in E. coli Isolates along the Production and Supply Chain of Pork around Hubei Province of China
Sher Bahadar Khan, Zou Geng, Cheng Yu-ting, Asad Sultan, Mumtaz Ali, Irshad Ahmad et al.
Using MacConkey agar, E. coli were isolated from 285 samples including 125 tonsil swabs (4-6 weeks old healthy pigs from 5 farms), 80 tissue samples from different slaughter houses (20 each intestine, liver, meat and kidney), and 80 samples from super- and wet…
3 Jun 2019DOI 10.17582/journal.pjz/2019.51.4.1569.1574
Research ArticleGenetic Homogeneity among Bull Sharks Carcharhinus leucas in the South China Sea
Zhaochao Deng, Jingchen Chen, Na Song, Yongzhen Li, Zhiqiang Han
Carcharhinus leucas has a cosmopolitan distribution and marked genetic structure. However, analysis of this structure has not included the South China Sea population. In the present study, we collected a total of 23 individuals from Xisha, and Nansha Islands i…
1 May 2019DOI 10.17582/journal.pjz/2019.51.4.1281.1288