Jia-xiang Wang1,2,* and Peng Li1,2
...investigated using immunohistochemistry. Our results indicate that the pancreas is divided into twosections: the exocrine and endocrine portion.The exocrine portionis comprised of peripheral gland cells and centroacinar cells; the endocrineportion consists of islet cells. Ghrelin-immunopositive (ghrelin-ip) cells were found in both the pancreatic exocrine portion and islets. The greatest number of ghrelin-ip cells was found in the islets, and the cell density ...
LX Ye1,2, JX Wang1,2,*, P Li1,2 and XT Zhang1,2
...investigated using immunohistochemistry. The testes of African ostrich consisted of a capsule and parenchyma. The capsule was divided into three annular layers, and the parenchyma consisted of tubular compartments and interstitial tissue. The tubular compartments were comprised of the seminiferous tubules, straight seminiferous tubules and rete testis, without any distinct boundary tissue between them. The interstitial tissue between the tubules contained many...
Xiaoting Zhang1,2, Jiaxiang Wang1,2,*, Peng Li1,2,* and Lixun Ye1,2
...investigated using immunohistochemistry. The results indicate that the lung of African ostrich was composed of interstitial tissue and parenchyma. The interstitial tissue formed a layer of serosa on the surface of the lung. The parenchyma of the lung consisted of bronchial levels, atria and respiratory capillaries. The bronchi were divided into the primary, secondary and tertiary bronchi. The wall of the tertiary bronchus had openings for the atria and respira...
Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*
...ls was detected by immunohistochemistry. The results showed that the expression of AR mRNA was found in the infundibulum, magnum, isthmus, uterus and vagina of the oviduct. The expression of the AR mRNA in infundibulum was the strongest, and the vaginal expression was weak. The immunohistochemical results was consistent with the molecular expression of AR mRNA, the positive reaction in the infundibulum, isthmus and uterus is more intense, followed by the magnu...
Yanjie Guo1, Weini Wu1, Hongmei Wang2, Xiao Guo2 and Yongping Xu2,*
... pathways, we used immunohistochemistry, RT-PCR, Western blot and in situ hybridization to detect the expression and distribution of IL-18Rα in the inferior mesenteric ganglion of the female goat. Our results showed that IL-18Rα mRNA and proteins are expressed in the IMG, which provides molecular evidence for the study of immune and neuro-modulation in the female reproductive system.
...
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
... with immunofluorescence histochemistry, Gene CD44, CD29, CD73 and SH2 are detected positive with RT-PCR. We carry karyotype analysis out and find that chromosome is 2n=78 and shows normal. CAFSCs in mid period of subculture propagate fastest from cell cycle examination on Flow Cytometer and growth curve using CCK-8 kit on ELIASA. CAFSCs are induced to neuron and epithelium (epiblast), adipocyte (mesoblast), hepatocyte (entoblast).
...
Shaher Bano1, Nadia Naseem2 and Sarah Ghafoor1,*
... for comparison of immunohistochemistry for TGF-α expression at week five and week nine. Expression of TGF-α was noted inthe ductal epithelial cells of SLG that was found to be comparatively increased at the age of five weeks and reduced at age of nine weeks. The expression of TGF-α between both glands at age of nine weeks showed a decreased expression in SLG as comparedto that of SMG.A decrease in the expression of TGF-α in SLG occurs ...
Jinfeng Liu1,2, Yanhong Cao3, Tong Feng1, Laiba Shafique1, Chan Luo1, Peng Zhu1,4,* and Qingyou Liu1,*
...at blastula stage. Immunohistochemistry exhibited BMP15 protein was located in germ cells of testis, in primordial granulosa cells, primary, secondary, and antral follicles of ovary, and none in theca cells. The more conspicuous reaction for BMP15 was observed in germ cells than cumulus cells and granulosa cells, particularly in primordial germ cells of genital ridge or in foetus ovary of buffalo. The expression pattern of BMP15 suggested ...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
... the most serious. Immunohistochemistry results demonstrated the detection of B. canis antigens in the lesions of all examined tissues. Strong positive staining was primarily found in the spleen, liver, and testicle. In conclusion, this study was the first to report the isolation of two B. canis strains from pet dogs in Sichuan province, southwestern China, and to further evaluate B. canis antigen location in tissues. Our study will contri...
Gong Ga1,2 and SuoLang Sizhu2,*
...cted in tissues by immunohistochemistry analysis. Overall, we found that that eight serum samples (4.6%, 8/173) were positive to anti-HEV IgM antibody and Seventeen serum sample (9.8%, 17/173) were positive for anti-HEV IgG antibody. The HEV RNA positive rate was of 4.6% (8/173) in feces and serum. Moreover, the prevalence of HEV RNA was 9.5% (2/21) in swine liver, 4.8% (1/21) in swine spleen, 9.5% (2/21) in swine kidney, 4.8% (1/21) in swine intesti...
Fei Wang1, Xianlai Zhang1, Youbao Zhong2, Xiaoen Tang3, Xiaofen Hu1,
ShanShan Yang1, Shengwei Zhong1, Zuohong Zhou1, Jin Liu1, Xu Yuan1
and Yong Li1*
...P) was detected by immunohistochemistry staining and RT-qPCR. In present results, the intestinal mucosa epithelium is a single layer of columnar epithelium, other structures are relatively complete, and the intestinal villi and glands are distinct in each part of intestine. The localization of VIP is extensive in the intestinal wall, but most typical in the lamina propria and muscular layer; VIP in mucosal epithelium is mainly distributed within goblet cells. ...
Xuya Zhou1, Ying Liu1, Deqin Xu1, Jie Bao1, Yaru Cao2 and Yong Jin3*
...A1 was detected by immunohistochemistry. Enzyme-linked immunosorbent assay (Elisa) was used to detect the prostaglandin E2 (PGE-2), 20-Hydroxyeicosatetraenoic acid (20-HETE), endothelin 1 (ET-1) and B-type natriuretic peptide (BNP) in blood serum of diabetes complicating arthritis rats. Blood pressure was measured by a noninvasive caudal artery blood pressure meter. Western blot analysis showed that unification of two drugs caused protein expression levels of ...
Xuhai Wang1,2, Xin Li1, Fangyuan Yuan1, Chaocheng Li1, Bin Jia1* and Song Jiang1*
...ally in sheep, the immunohistochemistry, in-situ hybridization was used to study the relationship and the distribution between miR-216b and IL2RB in the intestine tissue of kazakh sheep with resistant and non-resistant MHC haplotypes after oral infection with E. granulosus eggs. The results demonstrated that miR-216b negatively regulated IL2RB in the cystic echinococcosis intestinal tissues, and they were differentially expressed in the intestine tissue...
Mohammed Bashar Danlami1*, Basiru Aliyu1, Abbas Bazata Yusuf1, Bashar Gulumbe Haruna1 and Asmau Nono Mahe1
...ion of the facilities at histochemistry unit of FMC Birnin Kebbi....
HuaBin Zhang1,2, YaSen Li1, Tao Pan1, Peng Yan1, En Li1, Hui Xue1 and XiaoBing Wu1,*
...inese alligator by immunohistochemistry analysis. This study aims to access the possible effect of leptin in Chinese alligator. The results showed that immunoreactivity of leptin was observed in the adipocyte of white adipose tissue, the gastric gland of the stomach, the lamina propria of the intestine and the interstitial cell and seminiferous tubule of testis, leptin receptor staining was detected in the adipocyte of white adipose tissue, the gastric gland a...
Ou-Mei Hao1*, Xue-Feng Wang1, Zhi-Jun Yue1, Chun-Hong Nan1,Yan Yu1,Tong Zhang2,Yu-Feng Liu3 and Xue Zhao1
...s were assessed by Immunohistochemistry staining and Quantitative real-time (qRT)-PCR assays were performed to explore the effects of QZZKF on the expression of surfactant protein A (SP-A), B7 homolog 3 protein (B7-H3), interleukin (IL)-12 and IL-13. The expression levels of B7-H3 and IL-13 were upregulated after MPP infection in the mouse model (P<0.05), while the expressions of SP-A and IL-12 were significantly decreased (P<0.05). After five days of th...
Zekeriya Özüdoğru1, Hülya Kara2*, Adem Kara3, Derviş Özdemir3 and Hülya Balkaya2
... examined by using immunohistochemistry. CCK-IR, motilin-IR, ghrelin-IR, GIP-IR and VIP-IR cells were found to be more densely populated than other immunostained endocrine cells in the proventriculus and ventriculus sections. The distribution of CCK-IR, ghrelin-IR, and VIP-IR endocrine cells in the gastrointestinal tract of the red legged partridge was of an uncommon pattern. Our findings show that CCK-IR, motilin-IR, ghrelin-IR, GIP-IR and VIP-IR cells might ...
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
..., the H and E, and immunohistochemistry were done. The immunohistochemical test exposed the immunophenotype positive for CD79a. Finally, B-cell (CD79a) polycentric lymphoma was confirmed. This case report introduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practice in China, which can provide a reference for veterinarians in clinical diagnosis....
Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*
..., the H and E, and immunohistochemistry were done. The immunohistochemical test exposed the immunophenotype positive for CD79a. Finally, B-cell (CD79a) polycentric lymphoma was confirmed. This case report introduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practice in China, which can provide a reference for veterinarians in clinical diagnosis....
Taghreed Mohamed Nabil1, Randa Mohamed Hassan1, HebatAllah Hamdy Mahmoud2, Mohamed Gomaa Tawfiek2, Usama Kamal Moawad1*
Xiuli Wang1, Cuilan Hu2 and Sen Ye3*
...y TTC staining and immunohistochemistry. The effects of thrombectomy on brain wave fluctuation and GCSF in rats at different time points were measured according to electroencephalogram and RT-PCR. We found that the neurological function scores of rats decreased with the prolongation of thrombectomy time, and the scores of neurological function were lower in the 4.5h thrombolysis group than in the 6h thrombolysis group at the same time point. At the same time, ...
Wei Cui1, Yanwei Du1, Lijuan Jiang1, Yan Wei1, Yuguo Li1, Wenfeng Zhang1* and Ling Zhang2*
...hological changes. Immunohistochemistry showed that compared with the model group, the expression of Bcl-2 (B-cell lymphoma 2) in BHD group increased, while the expression of Bax (BCL2-associated X) and caspase-3 decreased. The expression of p-PI3K (phosphorylated phosphatidyl inositol 3- kinase), p-AKT (phosphorylated protein kinase B) and Bcl-2 decreased in model and HG group, while the expression of Bax, Caspase-3, and Cleaved Caspase-3 increased. After BHD...
Zhongxi Zhang1, Ping Zou2*, Jingfang Zhang1, Yujuan Zeng1 and Yujie Tang1
...n were detected by immunohistochemistry and Western blot. We found that compared with the model group, intravenous thrombolysis could significantly improve the behavioral disorder of rats, and significantly reduce the infarct size of rat brain tissue, and the expression levels of ICAM-1 protein and MMP9 protein. We conclude that intravenous thrombolysis could significantly improve the therapeutic effect of acute basilar artery occlusion in rats within 2-4 h, a...
Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1
...al Diarrhea Virus, Immunohistochemistry, Ovaries, Oviducts, PI cow, Uteri ...
Bnar Shahab Hamad1, Bushra Hussain Shnawa1*, Rafal Abdulrazaq Alrawi2
...paraffin underwent immunohistochemistry. Several immunolabeled liver sections with anti-CD3, CD4, CD8, CD20, CD68, and caspase-3 were examined under a light microscope. The histological alterations caused by the hydatid cyst were cell degeneration, necrosis, hemorrhage, congestion, and fibrosis. The findings revealed that the CD3 T cells were the most predominant inflammatory cell in hepatic mice tissue. Also, CD4 Helper T cells, CD8 Killer T cells, CD20 memor...
Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5
...histopathology and immunohistochemistry of collagen were performed. Results: Ivermectin was detected in 36 samples, out of them 15 contained high ivermectin levels (100 ppb). Chlorpyrifos, piperonyl butoxide, and acetamiprid were below the limit of quantification in 3 samples only. Histopathology of tick-infested skin revealed severe multifocal eosinophilic dermatitis and inflammation of subcutaneous hypodermis (panniculitis) whereas, in the skin with high ive...
Sen Ye1, Jiangang Pang2 and Xiuli Wang3*
...were examined. PAR immunohistochemistry was performed on brain sections after 24 h reperfusion. We found that compared with the rivaroxaban group, the warfarin pretreatment group had an increased ICH volume; PAR-1, -2, -3, and -4 were widely expressed in the normal brain and there was higher expression in the ischemic brain, especially in ischemic peripheral lesions. Warfarin pretreatment enhanced the expression of PAR-1 and PAR-2 in preischemic and post...
Thekra Fadel Saleh, Omar Younis Altaey*
... the anatomy, histology, histochemistry, and distribution of lymphoid tissue in the caeca of Muscovy ducks. Samples from eight healthy adult Muscovy ducks (Cairina moschata) were fixed, processed, sectioned, and stained with Hematoxylin and Eosin as well as Periodic Acid Schiff: Alcian blue pH 2.5 and toluidine blue stains for histochemical analysis. Dissecting microscope measurements and histological measures were taken, and immunohistochemical analysis was p...
Ibrahim Elmaghraby*, Abdel-Baset I. El-Mashad, Shawky A. Moustafa, Aziza A. Amin
Dalia M. Amin1*, Noha Mohammed Halloull1, Walaa E. Omar2, Basma A. Ibrahim2, Nahla M. Ibrahim1
...ibrosis as well as immunohistochemistry evaluation of Bcl2 and p53 expression in the kidneys were performed. In renal tissue, the concentrations of transforming growth factor-1, pERK1/2, and pSTAT3 were assessed. Results: The group that received adenine treatment had considerably higher blood urea nitrogen and serum creatinine levels as well as significant pathological alterations as tubular injury and infltration of infammatory cells. Elevated TGF1, pERK1/2, ...
Aryaf Mahmood Sabea1*, Majida A. Al-Qaiym2
... group for 4weeks. Immunohistochemistry analysis showed that expression of CD4, CD8, and CD19 on Tand B lymphocytes and CD68 on macrophages were affected inversely by hypothyroidism. Selenium supplementation synergies levothyroxine in reestablishment the activity of lymphocytes and macrophages. In conclusion, the present study demonstrated that thyroid hormones had regulatory role for innate and adaptive, and in order to reach the best result of treating hy...
Shafi Muhammad1, Bibi Nazia Murtaza2, Aftab Ahmad1, Muhammad Shafiq3, Nurul Kabir4 and Hamid Ali1*
...histopathology and immunohistochemistry (IHC) techniques in liver tissues in HCV patients from the southern districts of Khyber Pakhtunkhwa, Pakistan. In addition, the network of IL-17A and its KEGG pathway related gene IDs were constructed using GeneMANIA and STRING online web tools. IL-17A serum levels were significantly higher in fibrotic patients compared to chronic HCV patients and showed a positive correlation with age. The constructed gene network revea...
Yanmei Wang1,2, Haoyun Li1, Lijuan Han1 and Wenkui Wang1*
...stern blotting and immunohistochemistry. The results showed that, the mRNA expression of MIF, Raf, ERK, Akt, GSK3β, cyclin D1, Rb, E2F in PH group were significant higher than that of control. Moreover, the expression levels of MIF, p-Raf, p-ERK, p-Akt, p-GSK3β, cyclin D1, p-Rb, E2F proteins in PH group were significantly higher than that of the control. It can be concluded that, MIF participated in the occurrence of PH, possibly through Raf/ERK and ...
Rehan Ahmed Siddiqui1,2*, Shabana Usman Simjee2,3, Nurul Kabir4, Muhammad Ateeq3,5, Kevin Joseph Jerome Borges6, Muhammad Raza Shah3 and Rahman M. Hafizur2
...eas were measured. Immunohistochemistry for actin and cyclooxygenase-2 (COX-2) were also carried out. Real time RT-PCR studies were performed to check mRNA expressions of nuclear factor-κB (NFκB) p50, inducible nitric oxide synthase (iNOS), kidney injury molecule-1 (Kim-1) and hemeoxygenase-1 (HO-1). CA and CA-AuNPs improved blood urea and creatinine levels when compared with the glycerol induced AKI group. The compounds kept the actin intact, decr...
Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei
...investigations and Immunohistochemistry study. The results of this study showed oxidative stress elevation in Malon Di-Aldehyde concentration in testes, epididymis tissue treated with tetrodotoxin. The pathological lesion in testes of animals exposed to toxic dose of tetrodotoxin was characterized by obvious disruption of seminiferous tubules with absence of leydig cells and congestion, in epididymis tissue the most histopathological section showed widen...
Taghreed Jabbar Humadai*, Bushra Ibrahim Al-Kaisei
... liver tissue, The Immunohistochemistry study revealed an increase in the scoring of IL1 expression in liver that showed score (3) compared to control group, TNF alpha expression showed score (1) in liver tissue when compared to untreated control group . According to the findings of the current study, tetrodotoxin has adverse toxic effects on liver tissue of male albino rats.
Keywords | Tetrodotoxin, Pathological changes, Oxidati...
Jinzhao Li1, Yawen Zhang2, Binghan Jia1, Yuqiong Zhao1, Huijuan Luo1, Xiaojie Ren1, Yuan Li3, Xiaoyan Bai1, Jing Ye3 and Junping Li1*
...opulsion mobility, immunohistochemistry (IHC), western blot (WB) and ELISA for detecting levels of Tau, P-Ser396-tau, P-Ser404-tau, protein kinase C (PKC), p38 MAPK (p38), p-p38 MAPK (p-p38) and µ-opioid receptors (MORs) in the colonic samples. The gene sequencing approach was used to detect alternatively spliced tau isoforms in the distal colonic muscle layer. Sequencing analysis revealed that 3 isoforms of tau, namely 2N4R, 1N4R and 0N4R were expressed...
Rabab Abd Alameer Naser1*, Sameer Ahmed Abid Al-Redah2, Enas S. Ahmed3, Fatimah S. Zghair2, Ali Ibrahim Ali Al-Ezzy4
Hong Chen1,2, Jiawei Liu1,2, Chuan Lin1,2, Hao Lv1,2, Jiyun Zhang1,2, Xiaodong Jia1,2, Qinghua Gao1,2 and Chunmei Han1,2*
... PCR (qRT-PCR) and immunohistochemistry further indicated a higher expression of BVES in the mesenchymal tissues of regenerated antlers than in the antler skin, cartilage, and bone tissues after top pruning treatment (P < 0.05); the brown immunohistochemical reaction products were concentrated in the mesenchymal cell membranes and intercellular matrix of healthy and regenerated antlers. Our results suggested that the repair process of antler damage after to...
Islam S Alani*, Huda Sadoon Al-Biaty
...en used to conduct immunohistochemistry procedures. In normal biopsies, the expression of periostin was absent in the epithelial compartment. High levels of POSTN expression was detected in 93.2% of cancerous biopsies as well as in 16.7% of benignly diagnosed biopsies. With a noticeable difference between these two groups (P≤0.01). PN was found to be highly expressed in 92.1% of the newly diagnosed cases and 100% of the recurring cases. PN exhibited a highe...
Sherine Abbas1, Heba M.A. Abdelrazek2*, Eman M. Abouelhassan3, Nayrouz A. Attia4, Haneen M. Abdelnabi4, Seif El-Eslam E. Salah4, Abdelrahman M. Zaki4, Mohamed A. Abo- Zaid4, Nadia A. El-Fahla5
...met assay, splenic immunohistochemistry for interleukin-6 (IL-6), nuclear factor kappa B (NFKB) and tumor necrosis factor-alpha (TNF-α) and histopathology for spleen and thymus were performed. Oral administration of neem extract to pregnant female rats caused significant (P<0.05) declines in birth and final body weights of male offspring and their thymus absolute weight (P<0.05). It also caused significant neutrophil (%) promotion (P<0.05) and l...
Ashraf B. Said*, Ibrahim A. Ibrahim, Hoda I. Bahr, Marwa A. El-Beltagy
...topathological and Immunohistochemistry. Untreated diabetic group showed significant (P≤0.05) hyperglycemia, hyperinsulinemia, and elevation in inflammatory cytokines with hepatic and kidney tissues disruption. At the same time, treatment alleviated hyperglycemia and IR with a significant (P≤0.05) decrease in inflammatory biomarkers levels and tissue expressions, especially GLP-1 and Stem cell groups combined with metformin. The study concluded that whet...
