Search for Germplasm

Heterosis and Inbreeding Depression for Grain Yield Variables in Indigenous Maize Germplasm

Tauseef Taj Kiani1*, Mozammil Hussain1 and Hidayat ur Rahman2

...sion in indigenous maize germplasm comprising inbred lines NCMLQ₁, NCMLQ₂, NCMLD₁, NCMLD₂, NCMLD₃, and NCMLD₄. ese genotypes were crossed to get ten F₁ hybrid combinations, which were used to develop three generations F₂, BC₁ and BC₂. Heterosis (in F₁) and inbreeding depres- sion (in F₂, BC₁ and BC₂) were qu...

Shifted Multiplicative Model Clustering of Environments for Synthetic-Derived Bread Wheat

Fida Mohammad, O.S. Abdalla, Sheraz Ahmed, Fakharuddin and S. Rajaram

...g blood from T. tauschii germplasm were equivalent in performance to superior cultivars used in the study, thus can be utilized in breeding for drought stress by wheat breeders.

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Multivariate Analysis in Determining Morphologically Diverse Sugarcane Genotypes (Saccharum officinarum L.) and their Flowering Response at Arja, Azad Kashmir

Muhammad Shahzad Ahmed and Dilnawaz Ahmed Gardezi

...uction at new site. More germplasm need to be evaluated for selection of suitable parents that can be used for future breeding program.

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Screening of USDA Cotton Accessions against Sucking Insect Pests Complex and Cotton Leaf Curl Virus (CLCuV) Disease with Major Emphasis on Abiotic Factors

Syed Ishfaq Ali Shah^1,*, Tassawar Hussain Malik², Imran Rafi Khan¹ and Zahid Hussain³

...onducted to evaluate the germplasms imported from United State Department of Agriculture (USDA), United State of America (USA) primarily for Cotton leaf curl virus (CLCuV) disease with promising local varieties tolerant to the disease. After screening, the most resistant accessions of USDA were USG-14-2464 and USG-14-2478. However, USG-14-2481 and USG-14-2484 were recorded as partially resistant to CLCuV disease. Among the local

Honnur Basha, Vinaya Hemannavar, B.Ramanujam, R. Rangeshwaran and S.Sriram

Screening of chilli microflora and other biocontrol agents for their antagonistic effects on Colletotrichum spp. infecting chillies

...tes of yeasts from NBAII germplasm collection of biocontrol agents were tested for their antagonistic effect on Colletotrichum gloeosporioides and C. capsici by dual culture test. Among the isolates of chilli microflora tested, Aspergillus flavus showed 70.2% inhibition of C. gloeosporioides and 54.9% inhibition of C. capsici.Among the isolates of Trichoderma sp. tested, T.virens (Tvs-KSD isolate) and T. pseudokoningii (Tpk-1) showed highest percent inhibition...

Amitava Konar and N. Johnson Singh

Occurrence of aphids on various potato germplasms in eastern gangetic plains of West Bengal

...aphids on various potato germplasms. The common potato germplasms viz Kufri Chandramukhi, Kufri Jyoti, Kufri Jawhar along with three processing varieties like Kufri Chipsona-1, Kufri Chipsona-2 and Atlantic were screened against aphids. Potato seed tuber was planted by end of November following all standard agronomic practices except insecticide application. The aphid species viz. Myzus persicae (Sulzur) and Aphis gossypi Gl...

S. Choudhury and S. Pal

Population dynamics of mustard aphid on different Brassica cultivars under terai agro-ecological conditions of West Bengal

...acteristics of different germplasms. Brassica campestris varieties as a group harboured relatively higher populations of the aphid than Brassica juncea varieties.

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Identifying Stable Bread Wheat Derived Lines across Environments Through GGE Biplot Analysis

Sajid Muhammad^* and Fida Mohammad

Genetic Diversity in Cotton Germplasm using Multivariate Analysis

Syed Asif Imran Shah^1*, Shah Jehan Khan², Kalim Ullah¹ and Obaid Ullah Sayal²

...variability among cotton germplasm. Highest and close symmetry between phenotypic and genotypic coefficient of variances while lower values of environmental coefficient variance were observed for all the studied attributes which reflected that these traits were genetically controlled and under less influence of environment. Higher broad sense heritability and expected genetic advance reflected that all attributes were governed by additive genes and early pheno...

Stripe Rust: A Review of the Disease, Yr Genes and its Molecular Markers

Aqsa Waqar¹, Sahir Hameed Khattak², Sania Begum^2*, Tayyaba Rehman¹, Rabia¹, Armghan Shehzad², Wajya Ajmal², Syeda Shahdana Zia², Iqra Siddiqi²and Ghulam Muhammad Ali^2*

...y of rust genes in wheat germplasm and can facilitate the integration of multiple genes into wheat by pyramiding and transformation. This review discusses information regarding rust disease and resistance in wheat to tackle the disease through resistance breeding.

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Naheed Akhtar, Azra Nasim*, Ata ul Mohsin**, Muhammad Ashfaque, Shaheena Yasmin, Ghulam Jilani, Afzala Tashfeen, Waseem Ahmed Gilani, Irshad Begum* and Muhammad Munir***

...seedling bulk tests, the germplasm under study was grouped into three categories i.e., resistant, moderately resistant and susceptible. Data from seedling bulk tests showed that five wheat lines namely V- 01078, V-00055, KT-7, V-01180 and DN-47 were resistant with damage rating of 2- 3. Twelve wheat lines/varieties, 99B4012, 99B2278, V-9021, V-002493, Wafaq 2001, PR-84, TW0135, CT-00062, 7-03, PR-86, V-02192 and Chakwal 97 were found moderately resistant. Thre...

Naheed Akhtar, Noreen Moin, Ghulam Jilani*, Ata ul Mohsin**, Shaheena Yasmin, Afzala Tashfeen*, Munir Goraya**** and Irshad Begum*

...e used. Among the tested germplasm (one variety and 11 lines of wheat), V-5 line was found resistant and NRL-2017 line was found susceptible to Rhopalosiphum padi. The results of Antixenosis Test indicated that two wheat lines, V-5 and PR-83 were least preferred and three lines viz., NRL-2017, V-00055 and V-00BT004 were highly preferred by the test insect. The results of Antibiosis Test showed that PR-83 line was least fecund whereas NR-241 and V-00055 lines w...

Zaheer Ahmad, M. Yaqub Mujahid, M. Anwar Khan, Shaheena Yasmin, M. Asif and Maqsood Qamar*

... selected from an exotic germplasm nursery (INQYT 2003-04) received from CIMMYT, Mexico. It was evaluated for five years in different trials. It was first tested in advance yield trial conducted at NARC, then in Micro Yield Trials conducted at nine locations by BARI, Chakwal and finally it was evaluated in National Uniform Yield Trials conducted in rainfed ecology of Pakistan for two consecutive years. The NR-268 performed better than all other lines including...

Naheed Akhtar, Muhammad Ashfaque, Waseem Ahmad Gillani*, Ata-ul-Mohsin**, Afzala Tashfeen and Irshad Begum*

...ign: justify;"> The germplasm of National Uniform Wheat Yield Trials (Normal) (2003-04) were screened against Rhopalosiphum padi L., bird cherry oat aphid at National Agricultural Research Centre, Islamabad. Twenty National Uniform Wheat Yield Trials (NUWYT), Normal and 12 (NUWYT) rainfed varieties/ lines were evaluated for seedling bulk test to know the resistant, moderately resistant and susceptible wheat varieties/ lines. These results revealed that va...

Shazia Erum*, Muhammad Naeemullah, Shahid Masood** and Muhammad Irfan Khan*

...etic diversity in Ocimum germplasm and its suitability for commercial cultivation even in the area under small holdings, the investigation suggest its genetic as well as biochemical investigation for the production of commercial varieties and exploitation of the plant for economic benefits of the local communities.

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Habib Iqbal Javed, Ashiq Saleem, Javed Fateh, Mozammil Hussain, Naheed Akhtar and Shamim-ul-Sibtain Shah*

DEVELOPMENT OF RESISTANT MAIZE GERMPLASM

... comparatively resistant germplasm showed no leaf damage while 9 others had 7.10 - 44.7% infestation. In checks, the infestation was from 27.9 to 100.0%. The selected materials were again maintained by self pollination to make pure lines. During autumn 2001, selected material was screened under double artificial infestation, first at 3-4 leaf stage of the crop and the second 10 days after first infestation with 15 newly hatched larvae per plant every time. Des...

Ishaq Ahmad*, Abdul Rehman**, Ehsan –Ul- Haq*** and Arif Mahmood****

RESISTANCE RESPONSE OF RICE ( L.) GERMPLASM AGAINST RICE LEAF FOLDER ( G.) UNDER GREENHOUSE CONDITION IN PAKISTAN

...istani genotypes of rice germplasm including 15 coarse and 10 fine genotypes were screened in pot experiment during 2011 including TNI, used as susceptible check. The damage results revealed that one genotype PK-8893-4-1-3-1 was moderately resistant, seven genotypes were moderately susceptible, eleven genotypes found susceptible and seven genotypes were highly susceptible. The present study will facilitate the breeders in the development of leaf folder resista...

Genetic Analysis of Yield and Yield Components for the Improvement of Mungbean Germplasm

Ghulam Abbas¹, Muhammad Jawad Asghar¹, Muhammad Rizwan^2*, Muhammad Akram¹, Jaffar Hussain¹ and Fiaz Ahmad¹

... Improvement of mungbean germplasm for high seed yield requires information on genetic variability and correlation of traits. For this purpose, fifty eight exotic and indigenous diverse mungbean genotypes were evaluated for seed yield and other related traits. The genetic analysis of mungbean germplasm revealed high genotypic (GCV) and phenotypic (PCV) coefficients of variability for biological yield (GCV% = 31.70, PCV% = 33...

Effect of Lead (Pb2+) on Different Morpho-Physiological and Yield Traits of Mungbean (Vigna Radiata L. Wilzeck) under Water Deficit Conditions

Fiaz Ahmad¹, Mumtaz Hussain², Ghulam Abbas¹, Muhammad Jawad Asghar¹ and Muhammad Rizwan^3*

...06 proved to be a useful germplasm source against lead and water stress.

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Evaluation of Bottle Gourd Genotypes for Yield and Quality Traits

Muhammad Iqbal¹, Khalid Usman^2*, Muhammad Arif¹, Shakeel Ahmad Jatoi³, Muhammad Munir⁴ and Imran Khan¹

...assessment of the exotic germplasms investigated in this study displayed better performance over the local check in both the study years. The maximum germination (84.1 % and 85.4 %), number of fruits (25.4 and 23.9), fruit length (52.8 and 51.5 cm), fruit width (16.7 and 17.1 cm), fruit weight (1283.8 and 1330.2 g), yield (48.1 and 50.1 tons ha-1), TSS (7.1 and 6.9 %) and Vitamin C (16.1 and 16.1 mg/100g) were recorded in cv. Anmol. On the basis of overall per...

Study of Genetic Diversity in Pakistani Germplasm of Sesame (Sesamum indicum L.)

Mubashir Ahmad Khan*, Nazakat Nawaz, Muhammad Arshad, Muhammad Ayub Khan, Tahira, Doulat Baig, Ihsan Ullah Khan and Shamim ul Sibtain Shah

...ty in this set of sesame germplasm which could be utilized for crop improvement. It is further recommended that for making improvement in sesame crop, identified superior sesame lines viz. SG-115, SG-103, SG-78, SG-79, SG-31, SG-117, SG-108, SG-41 and SG-1 could be used in breeding program and further by making selections in segregating generations. This will help to increase sesame crop yield in the country.

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Genetic Analysis of Lentil (Lens Culinaris) Exotic Germplasm to Identify Genotypes Suitable for Mechanical Harvesting

Muhamad Jawad*, Shahid Riaz Malik, Muhammad Aqeel Sarwar, Muhammad Asadullah, Israr Hussain and Rabia Khalid

Identification of Resistance Sources in Wheat to Brown and Yellow Rust

Ateeq ur Rehman¹, Syed Atif Hasan Naqvi^1*, Ummad-ud-Din Umar¹, Muhammad Irfan Zafar¹, Faqeer Hussain², Muhammad Asif Zulfiqar³ and Azhar Ali Khan³

...mong the available wheat germplasm in Pakistan against the deadly rust disease. 152 lines of the wheat were screened against brown and yellow rust of wheat which are a serious pathosystem of wheat throughout the world. Some varieties were found resistant some were found moderately resistant while some were in susceptible response against the both rust disease. No variety was found to be resistant at all against the rust pathosystems. Correspondingly, in the re...

Optimization of Inoculation Technique of Sporisorium scitamineum for the Induction of Smut Disease in Sugarcane Propagative Material

Muhammad Aslam Rajput^1,3, Imtiaz Ahmed Khan², Rehana Naz Syed³ and Abdul Mubeen Lodhi^3*

... materials; standardized germplasm screening protocols as well as optimized inoculation techniques are required. The pathogen inoculation method should be less cumbersome, simple, and rapid. Here, we compared the effectiveness of six different inoculation methods, i.e., dipping, paste, wound+paste, soil infestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was significantly influenced by pathogen ...

Field Based Variability in Oat Crown Rust (Puccinia coronata f. sp. Avenae) Resistance in Exotic Oat Germplasm

Manzoor Hussain¹, Muhammad Awais², Farhatullah³, Saiqa Bibi¹, Muhammad Rameez Khan³, Afifa Khalid¹ and Sajid Ali^3*

...nce response, the tested germplasm was clustered into four groups i.e., G1 contained eight, G2 contained three, G3 contained two and G4 contained three genotypes. Group 1 could be regarded as the most resistant genotypes group, and contained genotypes O-SA-1, O-SA-5, O-SA-15, O-SA-10, O-SA-14, O-SA-4, O-SA-6 and O-SA-11. Genotype O-SA-9, O-SA-7 and O-SA-12 had relatively high crown rust susceptibility. Further studies on across location and over-years crown ru...

Heterosis Expression Analysis and its Impact on Different Agro-Morphological Characters in Sunflower (H. Annuus L.) Hybrids

Ihsan Ullah Khan*, Muhammad Arshad, Muhammad Ayub Khan, Muhammad Ashraf, Ashiq Saleem, Sundas Awan, Samra Azam and Shamim Ul Sibtain Shah

...genetically more diverse germplasm is incorporated in breeding programs.

...

Genotype by Environment Interaction in Bread Wheat Across Dry land Environments

Muhammad Ilyas* and Fida Mohammad

...erefore, newly developed germplasm or genotypes needs to be evaluated in multi-environment trials (METs) to know yield potential and adaptability. This experiment was designed to interpret GE interaction using 55 F5:8 bread wheat recombinant inbred lines (RILs) with five checks. Experimental material was planted using alpha lattice design in two replicates at Peshawar (E1and E5), Kohat (E2 and E6), Sarai Naurang (E3 and E7) and Dera Ismail Khan (E4 and E8) (Kh...

Study of Northern Corn Leaf Blight (NCLB) on Maize (Zea mays L.) Genotypes and its Effect on Yield

Tahsin Razzaq*, Muhammad Fareed Khan and Shahid Iqbal Awan

...y susceptible (S) within germplasm and had highest values for pathological traits with severity rating of 90S, 90S and 80S respectively. In second field trail (June 2017) crosses were made between selected parental genotypes in order to get F₁ progeny and evaluation of F₁ progenies and their parents was done in June 2018 for morpho-pathological traits to assess the impact of NCLB on yield loss and yield related attributes. Results showed ...

Isolation and Characterization of 25 Polymorphic Microsatellite Markers of Sepiella japonica

Jiaxin Guo¹, Xiumei Zhang^1,2, Xiaoyan Wang², Liqin Liu² and Tianxiang Gao^1,*

... be useful in evaluation germplasm and genetic composition in the further research.

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Primary Evaluation of Seed Characteristics of Common Bean Landraces Collected from Himalaya Region of Pakistan

Iffat Nawaz^1*, Farhatullah¹, Fida Muhammad¹, Sajid Ali² and Ghulam Muhammad Ali³

...rization of this diverse germplasm is recommended for future breeding programmes.

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Evaluation of Spring Wheat Genotypes for Climatic Adaptability using Canopy Temperature as Physiological Indicator

Muhammad Sohail^1*, Imtiaz Hussain², Maqsood Qamar², Sikander Khan Tanveer², Syed Haider Abbas², Zeshan Ali¹and Muhammad Imtiaz³

... and heat tolerant wheat germplasm under field conditions.

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Genetic Divergence and Heritability Studies for Yield and Yield Attributes in Various Accessions of Desi Chickpea (Cicer arietinum L.)

Muhammad Mohibullah¹*, Mehran¹, Sundas Batool¹, Muhammad Amin¹, Zakiullah¹, Muhammad Ilyas², Irfanullah¹, Abdur Rehman¹and Sardar Ali³

...re noted in the chickpea germplasmsin days to germination, days to 50% flowering, seeds per pod,1^st branches plant^-1, 2^ndry branches plant^-1, plant height, hundred grain weight and grain yield plant^-1. Overall, chickpea accessions CH30/12, CH10/12, CH44/12, CH14/12, and CH11/12 performed well, and recorded with maximum values for grains pod^-1, hundred seed weight along with other contributing pa...

Rao Wali Muhammad¹, Hafiz Muhammad Wasif Ali²*, Amir Hamza¹, Muhammad Qadir Ahmad², Abdul Qayyum², Waqas Malik² and Etrat Noor²

Estimation of Different Genetic Parameters in Various Safflower (Carthamus tinctorius L.) Genotypes under Field Condition

...ers and to partition the germplasm into various cluster groups on basis of their mean performance. A value of 74% was indicated by the first three PCs, which showed the highest variability between studied parameters. Eigen values >1 contributed variability between the genotypes under field condition. Minimum and maximum heritability estimates ranged between 77.4% - 99.0% for days to flowering and pods per plant, respectively. Genetic advance estimates range...

Huma Abbas¹, Muhammad Azhar Iqbal², Muhammad Kamran³, Muhammad Umar Shahbaz³*, Haseeb Ullah Kamber¹, Nazir Javed¹, Muhammad Junaid¹, Hira Abbas⁴ and Muhammad Ehetisham ul Haq³

Evaluation of Advanced Mung Bean Germplasm against Cercospora Leaf Spot and its In-vitro Management by Different Fungicides

...iseases through tolerant germplasm and with environmentally safe fungicides is a cost-effective approach. The present study was aimed to find the resistant germplasm against the disease and to evaluate the relative efficacy of different fungicides against the C. canesens in lab conditions. Twenty varieties/lines were screened against Cercospora Leaf Spot disease in field conditions. No line/ variety was found immune and only...

Attiq ur Rehman^1,2*, Iftikhar Hussain Khalil³ and Ihtisham Ali⁴

Genetic Diversity and Traits Association in Tetraploid and Hexaploid Wheat Genotypes in Khyber Pakhtunkhwa Province of Pakistan

... harvest index in tested germplasm. Durum genotypes DWE3 and DWE7 performed best for yield contributing traits while spring wheat verities, Janbaz, Barsat and Shahkar outperformed others in terms of 1000-grain weight, grain yield, and harvest index, respectively. These genotypes are recommended to be further tested at multi-locations to check for wider adaptability and a possible use in future wheat breeding programs in the area.

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Khan Sher¹*, Muhammad Subhan¹, Muhammad Nisar², Ali Hazrat², Zahid Fazal¹, Gul Rahim², Imran Ahmad¹, Riaz ul Haq¹ and Shamia Bibi₁

Genetic Diversity in Common Beans (Phaseolus vulgarus L.) Collected from Different Ecological Zones of Malakand Division (A Part of the Sino Japanese Region of Pakistan)

...rrelation in the present germplasm. The dendrogram in one-way cluster analysis distributed 55 lines into 2 lineages (I and II). The former was comprised of 24% bean landraces, while the latter was comprised of 76% landraces. In correlation coefficients 45 combinations were identified having strong positive correlation. The strongest correlations (above 50%) were computed 67% of all the combinations. The principal component analysis (PCA) identified quantitativ...

Shahid Ahmed^1,2*, Huiqing Su¹, Xuqian Sun¹, Peng Feng¹, Yongpan Chen¹, Liang Yu¹, Qian Liu¹ and Heng Jian¹

Performance of Cereal Varieties against Cereal Cyst Nematode (Heterodera avenae)

...jor cereal varieties and germplasm being adopted for cereal crop production annually. We tested number of cereal accessions to see the host pathogen interaction; among 15 wheat lines from Zhonnguyuan Sun (CAAS) CIMMYT Beijing and 10 lines of oats from Jilin Province were screened against H. avenae. The results showed that 5 wheat and 7 oat lines ranked as resistant genotypes while 9 wheat and 3 oat lines were ranked as moderately resistant and 1 wheat line and...

Saba Aleem¹*, Mehvish Tahir², Iram Sharif³, Muqadas Aleem⁴, Muhammad Najeebullah², Ali Nawaz¹, Amina Batool¹, Muhammad Imran Khan¹ and Waheed Arshad¹

Principal Component and Cluster Analyses as Tools in the Assessment of Genetic Diversity for Late Season Cauliflower Genotypes

...ining the variability of germplasm is an indispensable assist for crop improvement strategies. Multivariate analyses such as cluster and principal component analysis measures the amount of genetic diversity in respect of several charaters and asseses the relative contribution of different traits to the total variation. In the present study, nineteen genotypes were evaluated for presence of genetic diversity through principal component and cluster analyses. The...

Genetic Variability, Traits Association and Path Coefficient Analysis in Advanced Lines of Groundnut (Arachis hypogaea L.)

Muhammad Jahanzaib¹*, Nazakat Nawaz¹, Muhammad Arshad¹, Haris Khurshid¹, Muzamil Hussain² and Shahid Ali Khan¹

Mineral Profiling of Chickpea Wilt against Fusarium oxysporum f.sp. ciceris

Hafiz Tassawar Abbas¹*, Tamoor Khan¹, Ghulam Khaliq², Muhammad Aqeel Sarwar³, Muhammad Rashid⁴, Intazar Ali⁵, Muhammad Abuzar Jaffar², Ghulam Ali Bugti⁶and Muhammad Waseem⁴

... respectively. Resistant germplasm contained greater concentrations of these minerals related to susceptible lines/varieties. Increased mineral contents in resistant plants build up the physiological and biochemical methods of the host plants which help to prevent the extent of pathogen.

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Wild Resource Diversity of Mytilus unguiculatus Reduced by Aquaculture in the Southeast China Sea

Yahong Guo^1,3, Zeqin Fu^1,3, Jiantong Feng^1,3, Chengrui Yan^1,3, Yingying Ye^1,3*, Kaida Xu² and Baoying Guo^1,3

... maintain a high-quality germplasm bank for M. unguiculatus.

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Impact of Selection on Distribution of Crop Duration Parameters in Chinese Wheat Hybrids

Zainab Iftikhar, Muhammad Arif, Iqbal Munir and Sajid Ali

... of Chinese wheat hybrid germplasm for cultivation across Pakistan. For this purpose, 416 hybrids were assessed for diversity in crop duration parameters in the year 2017-18 and a subset of better performing 108 hybrids were selected and assessed for subsequent assessment. During the first experiment of preliminary testing of 416 Chinese wheat hybrids along with 5 local checks tested during 2017-18, significant variability (P<0.01) was observed for days to ...

Screening of Elite Chickpea Germplasm against Ascochyta Blight under Controlled Conditions

Javed Anwar Shah¹, Azhar Iqbal¹, Muhammad Tariq Mahmood², Muhammad Aslam³, Muneer Abbas⁴ and Ilyas Ahmad⁵*

Evaluation of Common Beans (Phaseolus vulgaris L.) Land Races based on Qualitative Traits Derived from Himalayan, Andean and Mesoamerican regions

Iffat Nawaz¹*, Tahseen Zeb¹, Bibi Saima Zeb² and Javaria Sherani³

...l markers in maintaining germplasm purity.

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Genetic Studies for Detection of Most Diverse and High Yielding Genotypes among Chickpea (Cicer arientinum L.) Germplasm

Muhammad Tariq Mahmood¹*, Muhammad Akhtar², Kaiser Latif Cheema², Abdul Ghaffar³, Imtiaz Ali⁴, Muhammad Jahanzaib Khalid² and Zeshan Ali⁵

...n occurring naturally in germplasm is highly valuable resource of alleles that can be deployed for genetic improvement of a species. Screening of available genetic stock for detection of most diverse and high yielding genotypes is a pre requisite for a successful crop breeding program. For this purpose, a research experiment comprising of sixty-eight elite chickpea germplasm genotypes along with two commercial varieties were...

Advances in DNA based Molecular Markers for the Improvement of Fruit Cultivars in Pakistan-A Review

Ghassan Zahid^{1, 2}*, Sara Iftikhar³, Muhammad Umer Farooq⁴ and Shakeel Ahmed Soomro⁵

...improvement of the fruit germplasm in Pakistan. Additionally, they also have diverse applications in the area of genetic diversity, varietal identification studies, hybrid detection, disease diagnostics and sex differentiation by administering the powerful diagnostic polymorphism detection tools at specific loci and the genome level. This review focuses on exploring the application, recent advancements and future implications of DNA-based molecular markers wit...

Biodiversity Evaluation among Wild Jujube (Ziziphus nummularia (Burm. F.) Population in Thal Desert of Pakistan

Naseem Sharif^1,2*, Imran Muhammad Siqqique², Muhammad Kashif Raza³, Urwa Irshad¹, Muhammad Ikhlaq Khan⁴, Ammara Noreen⁴ , Muhammad Ahsan Qureshi¹, Mohsin Abbas⁵, Muhammad Maaz Aziz⁵, Sitwat Riaz⁵, Komal Aslam⁵ and Naseem Akhtar⁶

... treasure of wild jujube germplasm (Ziziphus nummularia (Burm. F.)), but currently due to deforestation, global climatic changing scenario and shifting attitude of farmers towards other cash crops, the specie is in high risk of extinction. In this study, sixteen naturally growing wild jujube accessions were analysed based on multivariate analysis. Significant diversity was counted for selected morphological and biochemical traits like leaf length (1.8-5.4cm), ...

Relative Resistance of Seed of Advance Genotypes of Cicer arietinum Against Callosobruchus Chinensis During Storage

Muhammad Waseem Akhtar¹*, Khalid Mahmood Khawar², Muhammad Naeem Akhtar³, Muhammad Rafique¹, Khalid Hussain⁴, Sharmin Ashraf¹ and Muhammad Fida Hassan¹

... to screen out available germplasm of chickpea against dhora beetle (Callosobruchuschinisensis). For this purpose, 8 genotypes of chickpea genotypes were taken from Ayub Agricultural Research Institute, Faisalabad. 1 kg of each genotype was taken in plastic jars. There were three replications of each genotype for each insect. Twenty adults of dhora beetle were discharged in each container which were enclosed with muslin cloth with rubber bands. The Jars were p...

Screening of Tomato (Lycopersicon esculentum Mill.) Germplasm for Chilling Stress Tolerance during Two Growing Seasons

Kokab Nazim¹*, Asghari Bano¹ and Ghulam Jellani²

Effects of Seed Physical Characteristics of Benin Soybean Germplasm on their Resistance to Callosobruchus maculatus Fabricius (Coleoptera: Bruchidae)

Yêyinou Laura Estelle Loko¹*, Joelle Toffa¹, Azize Orobiyi¹, Gbèblonoudo Anicet Dassou², Rolande Okpeicha¹, Dieudonné Gavoedo¹ and Alexandre Dansi²

Biodiversity Estimation in Chilli (Capsicum frutescens) Germplasm through Morphological Traits

Ishtiaq Ahmad^1,2* and Muhammad Akbar Anjum¹

Association among Yield and Grain’s Physical Attributes in Spring Wheat Genotypes

Hafiz Ghulam Muhu-Din Ahmed¹*, Aziz Ullah², Muhammad Asim Bhutta³, Ammara Yasmeen⁴, Hafeez ur Rehman⁵ and Umar Farooq⁵

...these indices in studied germplasm. It was therefore suggested that positively and significantly correlated traits can be used for future breeding programs. The genotypes G33 followed by G20 and G22 well performed while the genotypes lowest performed G19 followed by G15 and G13 in yield and grain’s physical attributes in the current experiment. It will help the breeders for the selection of high yielding genotypes by only identifying plant yield and phys...

Estimation of genetic relationship of wheat germplasm against cereal cyst nematodes using RAPD technique

Y. I. Erum and F. Shahina†

...ic diversity among wheat germplasm (20 cultivars/lines) in

relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA

(RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum

percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and

molecular ...

Assessment of Heritability, Variability and Genetic Advance for Yield Linked Factors Related to Diversify Psyllium (Plantago ovate L.) Germplasm in Arid Conditions

Malik Muhammad Yousaf¹, Wali Muhammad¹, Muhammad Mohsin Raza¹*, Mumtaz Hussain¹, Muhammad Jahangir Shah¹, Bashir Ahamad¹, Annum Sattar², Hera Gull³, Sonia Sumreen⁴, Malik Waqar Yousaf⁵, Nazakat Nawaz⁶ and Nazim Hussain⁷

...that sorting of superior germplasm may direct the quick heritable improvement of the material.

...

Assessment of Heritability, Variability and Genetic Advance for Yield Linked Factors Related to Diversify Psyllium (Plantago ovate L.) Germplasm in Arid Conditions

...that sorting of superior germplasm may direct the quick heritable improvement of the material.

...

Development of Chance Seedling Varieties of Date Palm from Exotic Cultivars at Agro-Climatic Conditions of Faisalabad, Pakistan

Muhammad Zahid Rashid¹, Amina¹*, Hafiz Wasif Javaad², Muhammad Asim Rashid³ and Amina Rashid³

...all the exotic date palm germplasm investigated, Amber and Mabroon were found best in the characteristic under study.

...

Genetic Diversity Evaluation of Two Loach Fishes and their Artificial Hybrid Population Based on 19 Polymorphic Microsatellite Loci

Yanping Li, Fei Xu, Yongming Wang*, Yunyun Lv, Jinrong Shi, Biwen Xie, Wenyao Cai and Dian Liu

...sae ♂ as the potential germplasm resources for further artificial reproduction. Population structure analysis and UPGMA tree indicated S. superciliaris and S. superciliaris ♀ × S. reevesae ♂ were clustered together that comprised of clade I, and S. reevesae and S. superciliaris ♂ × S. reevesae ♀ were clustered together that comprised of clade II, suggesting the close genetic relationship between the offspring and their female parents. Thi...

Comparative Analysis of Genetic Variability and Heritability in Wheat Germplasms

Abdur Rauf¹*, Muhammad Sadiq¹, Farooq Jan¹, Muhammad Qayash², Wisal Khan³, Ikramullah Khan¹, Khilwat Afridi⁴, Muhammad Shuaib⁵, Muhammad Khalid⁶ and Samrin Gul⁷

...t exotic and eight local germplasms were sown in a three-replication randomized complete block design. The different parameters were recorded including days to heading, flag leaf area, plant height, tillers per plant, spike length, spikelets per spike, grain yield per plant, biological yield per plant, and 1000-grain weight. The analysis of variance showed significant differences for all the mentioned parameters (P ≤ 0.05). In local

Status of Wheat Germplasm Resistance Against Virulent Races of Leaf and Stripe Rust in Faisalabad, Pakistan

Muhammad Hussain¹, Yasir Ali²*, Baber Iqbal¹, Muhammad Azhar Iqbal¹, Salman Ahmad³, Muhammad Zeeshan Majeed⁴, Hafiz Muhammad Aatif², Muhammad Saeed¹ and Muhammad Jawad Yousaf¹

...ses, evaluation of wheat germplasm is still needed to reduce disease management costs and to increase crop sustainability. For this purpose, screening of 42 different wheat varieties and advanced lines was conducted at the Research area of Plant Pathology Research Institute, Ayub Agriculture Research Institute Faisalabad, during 3rd week of November 2019-2020 and 2020-2021 crop seasons. To monitor the changes of rust virulence pattern in the region, a set of r...

Compatible and Non-Compatible Interaction of Rice Germplasms against Rice Leaf Folder Cnaphalocrocis medinalis (Guenee) (Lepidoptera: Pyralidae)

Khwaja Junaid and Shah Alam Khan

...hermore, these resistant germplasms can be used in breeding programs for the development of high yielding resistant varieties against RLF.

...

AFLP based Breed Marker Present a Decree for Pakistani Sahiwal Cattle Breed Identification

Muhammad Haseeb Malik², Muhammad Moaeen-ud-Din¹*, Ghazal Kaukab Raja² and Feroza Hamid Wattoo²

...t of foreign animals and germplasm in Pakistan. It is becoming difficult to find pure males of Sahiwal breed for breeding purpose in public as well as private semen production units. The present study was designed to develop standard molecular markers for Sahiwal to ascertain their purity for breeding purpose. In this study 50 and 48 unrelated males were sampled for each Sahiwal and Crossbred cattle respectively. Candidate molecular markers present in Sahiwal ...

Genetic Analysis and Gene Mapping of Whitebacked Planthopper Resistance Genes from Rice Varieties

Baoxuan Nong^1,2, Biqiu Wu³, Anlong Xu², Wenai He² and Yongfu Qiu²*

...cation of WBPH-resistant germplasm and genes can promote the development of resistance varieties and effectively limit pest damage. In this study, fourteen varieties of rice were surveyed for insect resistance by assessing growth rates via seedbox screening, feeding activity via measurements of honeydew excretion, and insect development by counting the number of hatched nymphs. Two resistance varieties N22 and OB677 were crossed with susceptible line 9311 to d...

Exploring Genetic Diversity in Cotton Genotypes Using EST-SSR and ISSR Markers: A Comparative Study

Jaweria Iqbal¹, Muhammad Tanveer Altaf², Muhammad Faheem Jan³, Waqas Raza⁴*, Waqas Liaqat⁵, Ikram ul Haq⁶, Amna Jamil⁷, Sameer Ahmed¹, Amjad Ali² and Arif Mehmood⁸

...Studying existing cotton germplasm is crucial for discovering new genetic resources for future breeding. In this study, the effectiveness of EST-SSRs and ISSRs markers were compared for assessing genetic variation among 45 Pakistani Bt and non-Bt cotton varieties. ISSR and EST-SSR primers yielded 108 and 28 loci, respectively. The polymorphism was found 32.40% for ISSR, while 89.28% was recorded for SSR primers. Cluster analysis revealed a high level of geneti...

Genetic Diversity Estimation of Rice Genotypes based on Morphological and Quality Parameters through Principal Component Analysis

Salma Sharif¹, Rana Arsalan Javaid²*, Abid Majeed², Muhammad Shahzad Ahmed², Qurat ul Ain Sani², Faiza Siddique², Muhammad Arshad³ and Niaz Ali¹

...o assess indigenous rice germplasm using qualitative and quantitative features, as well as to select attractive genotypes for use in breeding programs in the future. A set of 65 accessions were evaluated in the field of Crop Sciences Institute, NARC following factorial design with three replications. highly significant differences were observed for all the quantitative traits as days to heading, days to maturity, plant height, number of tillers, panicle length...

Genetic Structure of the Population of Suri alpaca from Peru

Roberto Gallegos-Acero1, Ali William Canaza-Cayo2,4*, Francisco Halley Rodríguez-Huanca1, Rubén Herberht Mamani-Cato3, Abigail de la Cruz1, Júlio Sílvio de Sousa Bueno Filho4

Comparison of Variability in Exotic Genotypes of Strawberry (Fragaria × Ananassa duch.) in Climatic Conditions of Pakistan

Amina¹, Muhammad Zahid Rashid¹*, Muhammad Asim Rashid² and Amina Rashid²

...lts revealed that Sogoya germplasm have maximum survival (85.71%), number of leaves (16.55), leaf area (62.41 cm2), No. of flowers (38), No. of fruits (46), fruit length (41.69 mm), fruit weight (12.12) and yield (634.2g/plant) was included. While in qualitative characteristics germplasm Sogoya revealed highest total soluble solids (10.85%), total sugars (8.61%) and vitamin C (66.24 mg/100g). The outcomes of this study depic...

Phenotype Variety of West Sumatera’s Germplasm Randah Batu Kokok Balenggek Chicken

Firda Arlina¹*, Mangku Mundana¹, Linda Suhartati¹, Agmelia Jasfaria²

GERMPLASM DIVERSITY OF WHEAT UNDER DIFFERENT WATER STRESS AND WEEDY CONDITIONS

Nasir Mehmood,Muhammad Noor,Iftikhar Hussain Khalil,WAHEED MURAD,Muhammad Amin,Ihteram Ullah,Irfan Ahmad Shah

Genetic diversity and relatedness in exotic Mulberry (Morus) germplasm accessions

Syed Said Badshah Bukhari, Ghulam Ali Bajwa and Miskeen Ali

...p in ten exotic mulberry germplasm accessions. The results showed highly significant (p<0.01) variation in all agronomic traits. Both direct and indirect interactions were found among the traits. The highest genetic coefficient of variation (GCV) was 48.18% in total shoot length (TSL), while the lowest GCV was 3.27% in number of branches per plant (NB). The highest phenotypic coefficient of variation (PCV) was 48.19% in TSL, and the lowest PCV was 4.01% in moi...

Reducing poverty vis-a-vis combating salinity through reforestation in south Asia: a case study

Shams-ur-Rehman, Altaf Hussain and Riaz A. Khattak

...ike provision of quality germplasm of suitable species along with short term training in nursery and planting techniques. A bare minimum annual net income of US$ 80 to 90 per acre could be obtained if a 5-6 year old plantation of Eucalyptus camaldulensis (Source No. 15441) is sold to a nearby pulp mill. Simultaneously, growing nitrogen fixing tree species on these problematic sites in the region would not only improve fertility level but could also redu...

Age and Growth of Triplophysa (Hedinichthys) yarkandensis (Day, 1877) in the Tarim River in Xinjiang, China

Xinyue Wang¹, Shengao Chen^1,2*, Fangze Zi¹, Jianmin Ge¹, Desheng Chang¹, Yong Song¹ and Congxin Xie²

...r to exploit and develop germplasm resources of T. yarkandensis. Subsequent efforts should be made to prevent overfishing of young fish by limiting the minimum fishing individuals, and to protect older individuals by banning fishing during the breeding period.

...

Morphological Assessment of Resistance Potential in Tomato Against Early Blight Disease and Its Management

Aysha Fatima¹, Safdar Ali¹, Saira Azmat², Luqman Amrao¹, Muhammad Usman Ghani³*, Yasir Iftikhar⁴, Muhammad Ahmad Zeshan⁴*, Adeel Ahmad⁵ and Humaira Kalsoom⁶

...ce and nutrients. Tomato germplasm (Nadir F1, Naqeeb F1, Johir, Gohar, Walter Gaint FM9 and Money Maker) were screened against Alternaria solani to find out the resistance potential against early blight under field conditions. Data of disease severity and disease incidence was recorded fortnightly that showed only a single variety “Johir” expressed resistant (R) response out of 6 entries. The response of 2 varieties “Nadir F1 and Gohar”...

Effects of Different Combination of Fertilizer on Growth and Yield in Plantago ovata (Forsk.) Isapghol

Malik Muhammad Yousaf¹*, Abou Bakar Siddique¹*, M. Jahangir Shah¹, Mumtaz Hussain¹, Bashir Ahmad¹, M. Bilal Zafar¹, Maryam Hayat¹, Shamsa Rasool¹, Zainulabdeen¹, Muhammad Mohsin Raza³, Malik Ibrar Yousaf² and Usman Majeed¹

...022-2023. Five different germplasm were used for the study 21213, 21474, 20617, 21260 and 21988. The study was carried out to calculate the effects of chemical plant fertilizer include N and P (chemical fertilizing system), animal manure (Farmyard Manure), combination with chemical fertilizer (also known integrated fertilizer) at clay loam soil of AZRI, Bahawalpur during 2022-2023. Each germplasm contained following treatmen...

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Roberto Gallegos-Acero1, Ali William Canaza-Cayo2,4*, Francisco Halley Rodríguez-Huanca1, Rubén Herberht Mamani-Cato3, Abigail de la Cruz1, Júlio Sílvio de Sousa Bueno Filho4

Pakistan Journal of Agricultural Research

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Roberto Gallegos-Acero1, Ali William Canaza-Cayo2,4*, Francisco Halley Rodríguez-Huanca1, Rubén Herberht Mamani-Cato3, Abigail de la Cruz1, Júlio Sílvio de Sousa Bueno Filho4

Pakistan Journal of Agricultural Research

September

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